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Is Brucella excreted in cattle faeces? - Evidence from Punjab, India
This study aimed to isolate and identify the Brucella species from faeces of sero positive cattle in Punjab, India. Faecal samples were collected from 350 Brucella sero positive cattle in Ludhiana district of Punjab, India. Isolation was performed using a pre-enriched Brucella selective broth medium as well as Brucella selective medium agar plates containing horse serum and Brucella selective supplements. Isolates were identified using Gram staining technique and rapid slide agglutination test, and then confirmed by using bcsp31 and 16s rRNA genus specific PCR. Isolates were further identified up to species level by using ...
Source: Comparative immunology, microbiology and infectious diseases. - November 26, 2023 Category: Allergy & Immunology Authors: V Sharma P Kaur R S Aulakh R Sharma R Verma B B Singh Source Type: research

Serological and molecular survey of brucellosis and chlamydiosis in dromedary camels from Tunisia
Comp Immunol Microbiol Infect Dis. 2023 Nov 15;104:102098. doi: 10.1016/j.cimid.2023.102098. Online ahead of print.ABSTRACTThe present sero-epidemiological survey was designed and conducted to scrutinize the current status of camel-related brucellosis and chlamydiosis in Tunisia. Whole blood and serum samples were collected from 470 dromedaries (Camelus dromedarius) from eight different Tunisian governorates. Serum samples were subjected to indirect enzyme-linked immunosorbent assay (iELISA). The detection of Brucella and Chlamydia DNA was performed using conventional PCR targeting the bcsp-31 and 16 S rRNA gene, respectiv...
Source: Comparative immunology, microbiology and infectious diseases. - November 26, 2023 Category: Allergy & Immunology Authors: Rachid Selmi Aymen Mamlouk Hanene Belkahia Houcine Ben Yahia Hedi Abdelaali Mohamed-Habib Jemli Mourad Ben Said Lilia Messadi Source Type: research

APDDD: Animal parasitic diseases and drugs database
Comp Immunol Microbiol Infect Dis. 2023 Nov 15;104:102096. doi: 10.1016/j.cimid.2023.102096. Online ahead of print.ABSTRACTAnimal parasitic diseases not only have an economic impact, but also have serious social and public health impacts. Although antiparasitic drugs can treat these diseases, it seems difficult for users to comprehensively utilize the information, due to incomplete and difficult data collection. Thus, there is an urgent need to establish a comprehensive database, that includes parasitic diseases and related drugs. In this paper, we develop a knowledge database dedicated to collecting and analyzing animal p...
Source: Comparative immunology, microbiology and infectious diseases. - November 24, 2023 Category: Allergy & Immunology Authors: Yilei Zhang Guojun Chen Siyi Zhou Lingru He Olalekan Opeyemi Ayanniyi Qianming Xu Congshan Yang Zhenyu Yue Source Type: research

Enhancing leptospirosis control with nanosensing technology: A critical analysis
This article reviews nanomaterial development for an advanced leptospirosis detection method, e.g., latex beads-based agglutination test, magnetic nanoparticles enrichment, and gold-nanoparticles-based immunochromatographic assay. Thus, nanomaterials can be functionalized with biomolecules or sensing molecules utilized in various mechanisms such as biosensors. Over the last decade, many biosensors have been developed for Leptospira spp. pathogen and others. The evolution of biosensors for leptospirosis detection was designed for high efficiency and might be an alternative tool. In addition, the high-sensing fabrications ar...
Source: Comparative immunology, microbiology and infectious diseases. - November 22, 2023 Category: Allergy & Immunology Authors: Patcharapan Suwannin Kulachart Jangpatarapongsa Duangporn Polpanich Amani Alhibshi Abdelhamid Errachid Abdelhamid Elaissari Source Type: research

Organ distribution and early pathogenesis of Streptococcus equi subsp. zooepidemicus in swine
Comp Immunol Microbiol Infect Dis. 2023 Nov 18;104:102095. doi: 10.1016/j.cimid.2023.102095. Online ahead of print.ABSTRACTStreptococcus equi subsp. zooepidemicus is an emerging pathogen of pigs, resulting in high-mortality outbreaks of septicaemia and abortions. Here, we investigated the early pathogenesis of S. zooepidemicus in pigs following oronasal inoculation. Fourteen pigs were inoculated with live cultures of S. zooepidemicus ST-194, and monitored at 2,4, 8, and 24 h post-inoculation. Necropsies were performed to assess gross lesions and collect samples for bacterial culture and PCR analysis at each time point. Our...
Source: Comparative immunology, microbiology and infectious diseases. - November 21, 2023 Category: Allergy & Immunology Authors: Arthur Nery Finatto Sulove Koirala Fernanda Luiza Facioli J éssica Aparecida Barbosa Roman Nosach Matheus de Oliveira Costa Source Type: research

Occurrence of multidrug resistance associated with extended-spectrum β‑lactamase and the biofilm forming ability of Escherichia coli in environmental swine husbandry
Comp Immunol Microbiol Infect Dis. 2023 Nov 11;103:102093. doi: 10.1016/j.cimid.2023.102093. Online ahead of print.ABSTRACTExtended-spectrum beta-lactamase (ESBL) production and biofilm formation are mechanisms employed by Escherichia coli to resist beta-lactam antibiotics. Thus, we aimed to examine antibiotic resistance associated with ESBL production and biofilm formation in E. coli isolates from swine farms in Southern Thailand. In total, 159 E. coli isolates were obtained, with 44 isolates identified as ESBL producers, originating from feces (18.87 %) and wastewater (8.80 %) samples. All ESBL-producing strains exhibite...
Source: Comparative immunology, microbiology and infectious diseases. - November 17, 2023 Category: Allergy & Immunology Authors: Watcharapong Mitsuwan Sutsiree Intongead Phirabhat Saengsawang Chonticha Romyasamit Ruethai Narinthorn Veeranoot Nissapatorn Maria de Lourdes Pereira Alok K Paul Tuempong Wongtawan Ratchadaporn Boripun Source Type: research