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Sequencing methods for HA and NA genes of avian influenza viruses from wild bird feces using Oxford Nanopore sequencing
Comp Immunol Microbiol Infect Dis. 2023 Oct 4;102:102076. doi: 10.1016/j.cimid.2023.102076. Online ahead of print.ABSTRACTWe developed a method to determine the sequences of hemagglutinin (HA) and neuraminidase (NA) from RNA extracted directly from wild bird fecal samples, using Nanopore Flongle. We determined the nucleotide sequences and subtypes of HA and NA in 16 and 15 samples respectively, using Flongle. The results of HA and NA subtyping determined by the conventional method were consistent with their subtypes determined by our method, thereby the applicability of this method in the identification of HA and NA subtyp...
Source: Comparative immunology, microbiology and infectious diseases. - October 7, 2023 Category: Allergy & Immunology Authors: Kei Nabeshima Shingo Asakura Ritsuko Iwata Hisako Honjo Atsushi Haga Koichi Goka Manabu Onuma Source Type: research

Condemnation of bovine carcasses due to tuberculosis-gross lesions in Rio Grande do Sul, Brazil: Associated risk factors
This study aimed to identify risk factors for bovine tuberculosis (bTB) condemnation in cattle at slaughterhouses in Rio Grande do Sul, Brazil. A logistic regression analysis was conducted using data on bTB-related condemnations. Variables examined included animal origin, number of slaughtered animals, season, inspection level (state or municipality), animal sex, and slaughterhouse location. A total of 297,817 Animal Transport Guides were evaluated, representing the transportation of 3497,521 animals. Among these, 6097 (2.05%) had at least one animal condemned due to bTB lesions. Risk factors for condemnation included larg...
Source: Comparative immunology, microbiology and infectious diseases. - September 27, 2023 Category: Allergy & Immunology Authors: Jer ônimo Miguel Vicenzi Cristine Cerva Rog ério Oliveira Rodrigues Ang élica Cavalheiro Bertagnolli Fabiana Quoos Mayer Source Type: research

Dirofilaria immitis in pet dogs from the metropolitan area of the Colombian Caribbean
Comp Immunol Microbiol Infect Dis. 2023 Sep 18;102:102064. doi: 10.1016/j.cimid.2023.102064. Online ahead of print.ABSTRACTA cross-sectional study was carried out to determine the frequency and factors associated with Dirofilaria immitis infection in pet dogs in the metropolitan area of the Colombian Caribbean (northern Colombia). A total of 173 dogs were analyzed by a commercial rapid immunochromatographic test (RIT) and a nested PCR of the cytochrome oxidase subunit I gene, in parallel. Ninety-two (53.2%) of the dogs showed positive results to the RIT, while 59 (34.1%) animals had D. immitis DNA by PCR. Positivity to one...
Source: Comparative immunology, microbiology and infectious diseases. - September 23, 2023 Category: Allergy & Immunology Authors: Maria A Badillo-Viloria Ignacio Garc ía-Bocanegra Rodrigo J de Lavalle-Galvis Remigio Mart ínez Steffania de la Rosa-Jaramillo Adriana Castillo-Casta ñeda Juan D Ram írez David Cano-Terriza Source Type: research

Epizootological monitoring of some bacterial infectious diseases of animals on the territory of the Republic of Kazakhstan
Comp Immunol Microbiol Infect Dis. 2023 Sep 9;102:102061. doi: 10.1016/j.cimid.2023.102061. Online ahead of print.ABSTRACTThe presence of significant areas used for pastures in Kazakhstan makes it relevant to study the epizootic situation in terms of the incidence of animals during their grazing. The most frequent and dangerous diseases during this period are clostridiosis and listeriosis, which cause significant damage to livestock. Monitoring studies of the dynamics of morbidity among animals for infectious diseases during the grazing period were carried out on the basis of veterinary reporting. Bacteriological studies w...
Source: Comparative immunology, microbiology and infectious diseases. - September 23, 2023 Category: Allergy & Immunology Authors: Zhumagul Kirimbayeva Aspen Abutalip Assiya Mussayeva Gulnur Kuzembekova Natalya Yegorova Source Type: research

Characterization of Actinobacillus pleuropneumoniae isolated from pigs in Japan using whole genome sequencing
Comp Immunol Microbiol Infect Dis. 2023 Sep 9;102:102062. doi: 10.1016/j.cimid.2023.102062. Online ahead of print.ABSTRACTWe conducted whole-genome sequencing to investigate the serotypes, the presence of virulence and antimicrobial resistance genes, and the genetic relationships among isolates of Actinobacillus. pleuropneumoniae derived from diseased pigs. Serotype 2 (71.2%) was the most common, but the prevalence of serotypes 6 (13.6%) and 15 (6.8%) increased. Existing vaccines are considered ineffective on the isolates belonging to serotypes 6 and 15. The phylogenetic tree based on core genome single nucleotide polymorp...
Source: Comparative immunology, microbiology and infectious diseases. - September 23, 2023 Category: Allergy & Immunology Authors: Manao Ozawa Motoshi Kawano Hitoshi Abo Yukari Issiki Mio Kumakawa Michiko Kawanishi Akemi Kojima Shoko Iwamoto Source Type: research