The Protective Effects of 18 β-Glycyrrhetinic Acid on Imiquimod-Induced Psoriasis in Mice via Suppression of mTOR/STAT3 Signaling.
In conclusion, GA ameliorated the symptoms of psoriasis, at least in part, through inhibition of inflammatory cytokines and STAT3/mTOR signaling and activation of Tregs in both lymph nodes and spleens. These effects are expected to be beneficial in the treatment and prevention of psoriasis. PMID: 32908937 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - September 12, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Berberine Improves Inflammatory Responses of Diabetes Mellitus in Zucker Diabetic Fatty Rats and Insulin-Resistant HepG2 Cells through the PPM1B Pathway.
This study is aimed at investigating the effect of BBR and protein phosphatase, Mg2+/Mn2+-dependent 1B (PPM1B) on IR. Biochemical measurements and liver histopathology were detected using the biochemical analyzer and HE staining in ZDF rats, respectively. Microarray analysis of liver tissues was performed, and differentially expressed gene (DEG) levels were examined by quantitative real-time PCR (qPCR) and Western blot. Additionally, the effect of BBR was also explored in HepG2-IR cells. The glucose oxidase method and the fluorescent glucose analog were used to detect glucose consumption and uptake, respectively. The PKA i...
Source: Journal of Immunology Research - September 12, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Immune and Inflammation in Acute Coronary Syndrome: Molecular Mechanisms and Therapeutic Implications.
Authors: Wang H, Liu Z, Shao J, Lin L, Jiang M, Wang L, Lu X, Zhang H, Chen Y, Zhang R Abstract Acute coronary syndrome (ACS) is a major cause of acute death worldwide. Both innate and adaptive immunity regulate atherosclerosis progression, plaque stability, and thrombus formation. Immune and inflammation dysfunction have been indicated in the pathogenesis of ACS. The imbalance in the proatherogenic and antiatherogenic immune networks promotes the transition of plaques from a stable to unstable state and results in the occurrence of acute coronary events. The residual inflammatory risk (RIR) has received increasing...
Source: Journal of Immunology Research - September 12, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.
Authors: Liao H, Li Y, Zhang X, Zhao X, Zheng D, Shen D, Li R Abstract Objective. It has been shown that podocyte injury represents an important pathological basis that contributes to proteinuria and eventually leads to kidney failure. High glucose (HG) activates macrophage polarization, further exacerbating HG-induced podocyte injury. Our previous study on diabetic nephropathy rats indicated that thalidomide (Tha) has renoprotective properties. The present study explored the effects of Tha on mRNA and protein expressions of inducible nitric oxide synthase (iNOS), tumor necrosis factor- (TNF-) α, mannose rece...
Source: Journal of Immunology Research - September 12, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques.
This study provides a systematic, wide view of the immune components in the brain and peripheral organs for a deep understanding of the immune response after ischemic stroke. PMID: 32908941 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - September 12, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Identification and Functional Analysis of EPOR+ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis.
Conclusions: We have characterized TAMs expressing EPOR and CD163+EPOR+ macrophages as TAMs in osteosarcoma lung metastasis patients, which are highly associated with tumor aggressiveness. PMID: 32908942 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - September 12, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Comparative Analysis of the Occurrence and Role of CX3CL1 (Fractalkine) and Its Receptor CX3CR1 in Hemophilic Arthropathy and Osteoarthritis.
Conclusions: This study has demonstrated collective CX3CL1/CX3CR1 axis involvement in hemophilic arthropathy pathogenesis introducing new interesting diagnostics and a therapeutic target. PMID: 32884948 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - September 6, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Kangtaizhi Granule Alleviated Nonalcoholic Fatty Liver Disease in High-Fat Diet-Fed Rats and HepG2 Cells via AMPK/mTOR Signaling Pathway.
Authors: Zhang J, Du H, Shen M, Zhao Z, Ye X Abstract Kangtaizhi granule (KTZG) is a Chinese medicine compound prescription and has been proven to be effective in nonalcoholic fatty liver disease (NAFLD) treatment clinically. However, the underlying mechanisms under this efficacy are rather elusive. In the present study, network pharmacology and HPLC analysis were performed to identify the chemicals of KTZG and related target pathways for NAFLD treatment. Network pharmacology screened 42 compounds and 79 related targets related to NAFLD; HPLC analysis also confirmed six compounds in KTZG. Further experiments were a...
Source: Journal of Immunology Research - September 6, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Polymorphonuclear Cell Chemotaxis and Suicidal NETosis: Simultaneous Observation Using fMLP, PMA, H7, and Live Cell Imaging.
This study used in vitro live cell imaging to simultaneously observe and analyze PMN functions. To demonstrate this, the effects of phorbol-12-myristat-13-acetat (PMA, 0.1-10 nM), N-formylmethionine-leucyl-phenylalanine (fMLP, 10 nM), and protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H7) on PMN chemotaxis and suicidal NETosis were studied. PMA (1 nM-10 nM) resulted in significant concentration-dependent behavior in chemotaxis and an earlier onset of maximum oxidative burst and NET formation of up to 44%. When adding H7, PMA-triggered PMN functions were reduced, demons...
Source: Journal of Immunology Research - September 5, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Corrigendum to "Tumor-Derived CXCL1 Promotes Lung Cancer Growth via Recruitment of Tumor-Associated Neutrophils".
Corrigendum to "Tumor-Derived CXCL1 Promotes Lung Cancer Growth via Recruitment of Tumor-Associated Neutrophils". J Immunol Res. 2020;2020:5106904 Authors: Journal of Immunology Research Abstract [This corrects the article DOI: 10.1155/2016/6530410.]. PMID: 32879895 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - September 5, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Antiganglioside Antibodies and Inflammatory Response in Cutaneous Melanoma.
Authors: Ene CD, Tampa M, Nicolae I, Mitran CI, Mitran MI, Matei C, Caruntu A, Caruntu C, Georgescu SR Abstract Introduction: Endogenously produced antiganglioside antibodies could affect the evolution of cutaneous melanoma. Epidemiological and experimental evidence suggest "chronic inflammation" to be one of the hallmarks in skin cancers. The aim of the study was to characterize the relation between antiganglioside antibodies and inflammation in cutaneous melanoma focusing on gangliosides GM1, GM2, GM3, GD1a, GD1b, GT1b, GQ1b. Material and Method. We performed an observational study that included 380 sub...
Source: Journal of Immunology Research - August 30, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Salivary IL-17A, IL-17F, and TNF- α Are Associated with Disease Advancement in Patients with Oral and Oropharyngeal Cancer.
Salivary IL-17A, IL-17F, and TNF-α Are Associated with Disease Advancement in Patients with Oral and Oropharyngeal Cancer. J Immunol Res. 2020;2020:3928504 Authors: Zielińska K, Karczmarek-Borowska B, Kwaśniak K, Czarnik-Kwaśniak J, Ludwin A, Lewandowski B, Tabarkiewicz J Abstract Delayed diagnosis of oral cavity and oropharyngeal cancer is associated with a poor prognosis. Despite progress in systemic therapy and radiotherapy, there has only been a slight improvement in the five-year survival rate. A non-invasive diagnostic method that consists of an assessment of specific proteins in saliva ...
Source: Journal of Immunology Research - August 30, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Elevated Levels of Activated and Pathogenic Eosinophils Characterize Moderate-Severe House Dust Mite Allergic Rhinitis.
In conclusion, patients with moderate-severe AR are characterized by elevated levels of activated and pathogenic eosinophils, which are associated with higher production of ECP, EPX, and IL-4 in the peripheral blood. PMID: 32855977 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 30, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Elemental Diet Enriched with Amino Acids Alleviates Mucosal Inflammatory Response and Prevents Colonic Epithelial Barrier Dysfunction in Mice with DSS-Induced Chronic Colitis.
Conclusions: We showed that ED amino acids can be an effective immunomodulatory agent to reduce colitis-related inflammation by inhibiting proinflammatory mediators and Th1/Th17 cell responses and by repairing the disrupted epithelial barrier. PMID: 32855978 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 30, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Corrigendum to "Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Na ïve/Memory T Cell Immunomonitoring".
Corrigendum to "Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring". J Immunol Res. 2020;2020:2698258 Authors: Macchia I, La Sorsa V, Ruspantini I, Sanchez M, Tirelli V, Carollo M, Fedele G, Leone P, Schiavoni G, Buccione C, Rizza P, Nisticò P, Palermo B, Morrone S, Stabile H, Rughetti A, Nuti M, Zizzari IG, Fionda C, Maggio R, Capuano C, Quintarelli C, Sinibaldi M, Agrati C, Casetti R, Rozo Gonzalez A, Iacobone F, Gismondi A, Belardelli F, Biffoni M, Urbani F Abstract [This corrects the article DOI: 10.1155/2020/1938704.]. ...
Source: Journal of Immunology Research - August 29, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis.
Authors: Peixoto F, Nascimento MT, Costa R, Silva J, Renard G, Guimarães LH, Penna G, Barral-Netto M, Carvalho LP, Machado PRL, Carvalho EM Abstract Cutaneous leishmaniasis (CL) due to L. braziliensis is associated with an exaggerated inflammatory response and tissue damage. Miltefosine is more effective than pentavalent antimony (Sbv) in the treatment of CL, and here, we evaluate the ability of Sbv, miltefosine, and GM-CSF administered intravenously, orally, or topically, respectively, to modify the immune response. Patients were treated with miltefosine plus GM-CSF, miltefosine plus placebo, or Sbv. Mononu...
Source: Journal of Immunology Research - August 29, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of β-Catenin.
MPC1 Deficiency Promotes CRC Liver Metastasis via Facilitating Nuclear Translocation of β-Catenin. J Immunol Res. 2020;2020:8340329 Authors: Tian GA, Xu CJ, Zhou KX, Zhang ZG, Gu JR, Zhang XL, Wang YH Abstract Accumulating evidence has pointed out that metastasis is the leading cause of death in several malignant tumor, including CRC. During CRC, metastatic capacity is closely correlated with reprogrammed energy metabolism. Mitochondrial Pyruvate Carrier 1 (MPC1), as the carrier of transporting pyruvate into mitochondria, linked the glycolysis and TCA cycle, which would affect the energy productio...
Source: Journal of Immunology Research - August 29, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Igg-Dependent Hydrolysis of Myelin Basic Protein of Patients with Different Courses of Schizophrenia.
Authors: Parshukova DA, Smirnova LP, Kornetova EG, Semke AV, Buneva VN, Ivanova SA Abstract The level hydrolysis of myelin basic protein (MBP) by IgG in patients with schizophrenia was studied depending on the clinical features and course of the disease. The patients were grouped according to type of schizophrenia and type of disease course. We found that IgGs isolated and purified from sera of schizophrenia patients' blood hydrolyses human MBP, and the level of this hydrolysis significantly exceeds that of healthy individuals. Detection of protease activity corresponding only to intact IgGs in polyacrylamide gel f...
Source: Journal of Immunology Research - August 29, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus.
In conclusion, overexpression of FOXO3 is correlated with the ongoing islet autoimmune destruction and might suggest a potential role for this gene in the pathogenesis of type 1 diabetes mellitus. PMID: 32851102 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 29, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Adenosine-Induced NLRP11 in B Lymphoblasts Suppresses Human CD4+ T Helper Cell Responses.
We examined the regulation and mechanism of adenosine responsiveness via NLRP11 in human Daudi Burkitt's B lymphoma cells and their effects on human peripheral CD4+ T lymphocytes from healthy individuals. NLRP11 was significantly upregulated after induction with adenosine at both the mRNA and protein levels, which led to the interaction of endogenous NLRP11 with the ASC adaptor protein; however, this interaction did not result in the activation of the caspase-1 enzyme. Furthermore, cocultures of NLRP11-expressing Burkitt's lymphoma cells and naïve human peripheral CD4+ T lymphocytes had reduced IFN-γ and IL-17A ...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Detection of Dengue Virus-Specific IgM and IgG Antibodies through Peptide Sequences of Envelope and NS1 Proteins for Serological Identification.
In this study, linear epitopes of envelope (E) and NS1 proteins were identified to diagnose the DENV. Whole protein sequences of E and NS1 of DENV were obtained from UniProtKB database. On the basis of algorithm prediction from DNASTAR, BCEPRED, and IEDB data resources, twelve peptides of E (EP1 to EP12) and eight peptides of NS1 (NS1-1 to NS1-8) were selected, which were common in all serotypes. Sequence homologies of peptides with other Flavivirus were checked by Multiple Sequence Alignment Tool ClustalX2. Peptide sequences were synthesized chemically by solid-phase peptide synthesis technique. Dengue-specific IgM and Ig...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Diagnosis and Treatment of Rheumatic Adverse Events Related to Immune Checkpoint Inhibitors.
Authors: Xiao Y, Zeng L, Shen Q, Zhou Z, Mao Z, Wang Q, Zhang X, Li Y, Yao W Abstract Immune checkpoint inhibitors (ICIs) have completely changed the treatment of cancer, and they also can cause multiple organ immune-related adverse reactions (irAEs). Among them, rheumatic irAE is less common, mainly including inflammatory arthritis, rheumatic myalgia/giant cell arteritis, inflammatory myopathy, and Sjogren's syndrome. For oncologists, rheumatism is a relatively new field, and early diagnosis and treatment is very important, and we need to work closely with experienced rheumatologists. In this review, we focused on...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Akkermansia muciniphila Enhances the Antitumor Effect of Cisplatin in Lewis Lung Cancer Mice.
Authors: Chen Z, Qian X, Chen S, Fu X, Ma G, Zhang A Abstract Recently, intestinal flora plays a vital role in the occurrence and development of tumors and there is link between cancer immunotherapy and Akkermansia muciniphila (Akk). However, the therapeutic efficacy of Akk in lung cancer remained unclear. Hence, our study is aimed at investigating the antitumor effects of cisplatin (CDDP) combined with Akk on lung cancer. Using the murine lung cancer model by subcutaneously inoculating Lewis lung cancer model, 50 mice were divided into five groups: normal, model, CDDP, CDDP+Akk, and CDDP+antibiotics. After treatme...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells.
In this study, we examined the effect of CGRP on HDAC2-mediated gene transcription in microglial cells through the chromatin immunoprecipitation sequencing (ChIP-seq) method. Immunofluorescence analysis showed that mouse microglial cells (BV2) expressed CGRP receptor components. Treatment of microglia with CGRP increased HDAC2 protein expression. ChIP-seq data indicated that CGRP remarkably altered promoter enrichments of HDAC2 in microglial cells. We identified 1271 gene promoters, whose HDAC2 enrichments are significantly altered in microglia after CGRP treatment, including 1181 upregulating genes and 90 downregulating g...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Prealbumin-to-Globulin Ratio Can Predict the Chemotherapy Outcomes and Prognosis of Patients with Gastric Cancer Receiving First-Line Chemotherapy.
The objective of the present study was to examine whether pretreatment PGR is related to the prognosis and chemotherapy outcomes of in-patients with advanced GC undergoing first-line chemotherapy. We retrospectively reviewed the data of 281 patients with unresectable GC from January 2013 to January 2018. The receiver operating characteristic curve analysis determined the cut-off values for the PGR. The relationship between the PGR and chemotherapy effectiveness was evaluated using the chi-square test. Kaplan-Meier's method was used to plot progression-free survival (PFS) and overall survival (OS) curves, using multivariabl...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Profound Functional Suppression of Tumor-Infiltrating T-Cells in Ovarian Cancer Patients Can Be Reversed Using PD-1-Blocking Antibodies or DARPin ® Proteins.
Profound Functional Suppression of Tumor-Infiltrating T-Cells in Ovarian Cancer Patients Can Be Reversed Using PD-1-Blocking Antibodies or DARPin® Proteins. J Immunol Res. 2020;2020:7375947 Authors: Foord E, Klynning C, Schoutrop E, Förster JM, Krieg J, Mörtberg A, Müller MR, Herzog C, Schiegg D, Villemagne D, Fiedler U, Snell D, Kebble B, Mattsson J, Levitsky V, Uhlin M Abstract PD-1/PD-L1 blockade has revolutionized the field of immunooncology. Despite the relative success, the response rate to anti-PD-1 therapy requires further improvements. Our aim was to explore the enhancement ...
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

A Comparative Transcriptomics Approach to Analyzing the Differences in Cold Resistance in Pomacea canaliculata between Guangdong and Hunan.
This study presents the molecular mechanisms for the cold hardiness of Pomacea canaliculata, which could provide a scientific basis for the forecast and prevention of harm from Pomacea canaliculata. PMID: 32832573 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 26, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

CD40 Signaling Promotes CXCR5 Expression in B Cells via Noncanonical NF- κB Pathway Activation.
CD40 Signaling Promotes CXCR5 Expression in B Cells via Noncanonical NF-κB Pathway Activation. J Immunol Res. 2020;2020:1859260 Authors: Wei C, Chen Y, Xu L, Yu B, Lu D, Yu Y, Lei Z, Tang R, Zhou S, Zhu J, Chen X, Su C Abstract Chemokine receptor CXCR5-mediated control of B cell trafficking in the lymphoid tissues plays a central role in orchestrating the B cell function, which not only guides the colocalization of B cells with follicular helper T cells in the follicular mantle zone but also determines the position of germinal center dark and light zones. However, the mechanisms that regulate the...
Source: Journal of Immunology Research - August 19, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

The Gut Microbiota and Respiratory Diseases: New Evidence.
Authors: Chunxi L, Haiyue L, Yanxia L, Jianbing P, Jin S Abstract Human body surfaces, such as the skin, intestines, and respiratory and urogenital tracts, are colonized by a large number of microorganisms, including bacteria, fungi, and viruses, with the gut being the most densely and extensively colonized organ. The microbiome plays an essential role in immune system development and tissue homeostasis. Gut microbiota dysbiosis not only modulates the immune responses of the gastrointestinal (GI) tract but also impacts the immunity of distal organs, such as the lung, further affecting lung health and respiratory di...
Source: Journal of Immunology Research - August 19, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

High CXCR3 on Leukemic Cells Distinguishes IgHV mut from IgHV unmut in Chronic Lymphocytic Leukemia: Evidence from CD5high and CD5low Clones.
Authors: Manukyan G, Papajik T, Mikulkova Z, Urbanova R, Kraiczova VS, Savara J, Kudelka M, Turcsanyi P, Kriegova E Abstract Despite the shared pattern of surface antigens, neoplastic cells in chronic lymphocytic leukemia (CLL) are highly heterogeneous in CD5 expression, a marker linked to a proliferative pool of neoplastic cells. To further characterize CD5high and CD5low neoplastic cells, we assessed the chemokine receptors (CCR5, CCR7, CCR10, CXCR3, CXCR4, CXCR5) and adhesion molecules (CD54, CD62L, CD49d) on the CD5high and CD5low subpopulations, defined by CD5/CD19 coexpression, in peripheral blood of CLL pati...
Source: Journal of Immunology Research - August 19, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Anti-Inflammatory Activity of Diterpenoids from Celastrus orbiculatus in Lipopolysaccharide-Stimulated RAW264.7 Cells.
In this study, compounds 1-17, including undescribed podocarpane-type trinorditerpenoid (3), were purified from C. orbiculatus and their chemical structure were determined by high-resolution electrospray ionization mass (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopic data. To investigate the anti-inflammatory activity of compounds 1-17, nitric oxide (NO) secretion was evaluated in LPS-treated murine macrophages, RAW264.7 cells. Among compounds 1-17, deoxynimbidiol (1) and new trinorditerpenoid (3) showed the most potent inhibitory effects (IC50: 4.9 and 12.6 μM, respectively) on lipopolysaccharide- (...
Source: Journal of Immunology Research - August 19, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

The Application of Single-Cell RNA Sequencing in Vaccinology.
Authors: Noé A, Cargill TN, Nielsen CM, Russell AJC, Barnes E Abstract Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell...
Source: Journal of Immunology Research - August 19, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Mechanism of Follicular Helper T Cell Differentiation Regulated by Transcription Factors.
Authors: Ji LS, Sun XH, Zhang X, Zhou ZH, Yu Z, Zhu XJ, Huang LY, Fang M, Gao YT, Li M, Gao YQ Abstract Helping B cells and antibody responses is a major function of CD4+T helper cells. Follicular helper T (Tfh) cells are identified as a subset of CD4+T helper cells, which is specialized in helping B cells in the germinal center reaction. Tfh cells express high levels of CXCR5, PD-1, IL-21, and other characteristic markers. Accumulating evidence has demonstrated that the dysregulation of Tfh cells is involved in infectious, inflammatory, and autoimmune diseases, including lymphocytic choriomeningitis virus (LCMV) i...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Anti-HMGCR Specificity of HALIP: A Confirmatory Study.
This study confirms that the HALIP is due to human anti-HMGCR antibodies. PMID: 32766318 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Neurocognitive Function, Psychosocial Outcome, and Health-Related Quality of Life of the First-Generation Metastatic Melanoma Survivors Treated with Ipilimumab.
Conclusion: A majority of melanoma survivors treated with IPI continue to suffer from emotional distress and impairment in NCF. Timely detection in order to offer tailored care is imperative, with special attention for survivors with a history of neuroendocrine or neurological irAE. The trial is registered with B.U.N. 143201421920. PMID: 32775464 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Comprehensive Screening of Mouse T-Cell Epitopes in Human Herpesvirus 6B Glycoprotein H/L/Q1/Q2 Tetramer Complex.
Authors: Okutani M, Kawabata A, Nishimura M, Nagamata S, Kuwabara S, Haseda Y, Munakata L, Suzuki R, Mori Y, Aoshi T Abstract Human herpesvirus 6 (HHV-6) infects over 90% of people. The HHV-6 subtype, HHV-6B in particular, is often associated with exanthem subitum in early childhood. Exanthem subitum is usually self-limiting and good prognosis disease; however, some infants primarily infected with HHV-6B develop encephalitis/encephalopathy, and half of the patients developed encephalopathy reported to have neurological sequelae. Furthermore, after primary infection, HHV-6B remains in a latent state and sometimes re...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

The Role of MicroRNAs in Regulating Cytokines and Growth Factors in Coronary Artery Disease: The Ins and Outs.
In this study, besides providing a comprehensive overview of the involvement of cytokines, growth factors, and miRNAs in CAD, the interplay between miRNA with cytokine or growth factors during the development of CAD is discussed. PMID: 32775466 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Abnormal Expression of BTLA and CTLA-4 Immune Checkpoint Molecules in Chronic Lymphocytic Leukemia Patients.
Authors: Karabon L, Partyka A, Ciszak L, Pawlak-Adamska E, Tomkiewicz A, Bojarska-Junak A, Roliński J, Wołowiec D, Wrobel T, Frydecka I, Kosmaczewska A Abstract Chronic lymphocytic leukemia (CLL) is characterized by the peripheral accumulation of neoplastic B cells and is frequently complicated by the systemic immunosuppression associated with an impairment in B and T lymphocyte activation. We hypothesized that the expression of immune checkpoint suppressors B and T lymphocyte attenuator (BTLA) and cytotoxic T lymphocyte antigen (CTLA-4) is disturbed in both lymphocyte subpopulations in CLL. The expression of CTL...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

The Role of Gastric Mucosal Immunity in Gastric Diseases.
Authors: Nie S, Yuan Y Abstract Gastric mucosa plays its immune function through innate and adaptive immunity by recruiting immune cells and releasing corresponding cytokines, which have an inseparable relationship with gastric diseases. Whether infective gastric diseases caused by Helicobacter pylori, Epstein-Barr virus or other microbe, noninfective gastric diseases, or gastric cancer, gastric mucosal immunity plays an important role in the occurrence and development of the disease. Understanding the unique immune-related tissue structure of the gastric mucosa and its role in immune responses can help prevent gas...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Single-Cell Transcriptome Analysis Profile of Meniscal Tissue Macrophages in Human Osteoarthritis.
Authors: Zhou J, Zhao Z, He C, Gao F, Guo Y, Qu F, Liu Y Abstract Osteoarthritis (OA) has long been considered as a degenerative disease, but growing evidence suggests that inflammation plays a vital role in its pathogenesis. Unlike rheumatoid arthritis and other autoimmune diseases, inflammation in OA is chronic and, in relatively low grade, mainly mediated by the innate immune system, especially macrophages. However, due to its low abundance, there is a lack of systematic studies on macrophages in the OA condition. Here, we have used single-cell RNA sequencing analysis to gain insight into the heterogeneity and f...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Regulating the Polarization of Macrophages: A Promising Approach to Vascular Dermatosis.
Authors: Peng H, Xian D, Liu J, Pan S, Tang R, Zhong J Abstract Macrophages, a kind of innate immune cells, derive from monocytes in circulation and play a crucial role in the innate and adaptive immunity. Under the stimulation of the signals from local microenvironment, macrophages generally tend to differentiate into two main functional phenotypes depending on their high plasticity and heterogeneity, namely, classically activated macrophage (M1) and alternatively activated macrophage (M2). This phenomenon is often called macrophage polarization. In pathological conditions, chronic persistent inflammation could in...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Altered Regulatory B Cell Subsets in Children with Type 1 Diabetes Mellitus.
Authors: El-Mokhtar MA, Elsherbiny NM, Sayed D, Raafat DM, Askar E, Hussein A, Abdel-Malek MAY, Shalaby AM Abstract B regulatory cells (Breg) refer to characteristic subsets of B cells that generally exert anti-inflammatory functions and maintain peripheral tolerance mainly through their ability to secrete interleukin-10 (IL10). Dysregulation in the function of Breg cells was reported in several autoimmune diseases. However, the relation between Breg and children with type 1 diabetes (T1D) is poorly understood. Thus, this study is aimed at determining whether Breg cells play a role in T1D in children or not, so we ...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Role of Myokines in Myositis Pathogenesis and Their Potential to be New Therapeutic Targets in Idiopathic Inflammatory Myopathies.
Authors: Mageriu V, Manole E, Bastian AE, Staniceanu F Abstract Idiopathic inflammatory myopathies (IIM) represent a heterogeneous group of autoimmune diseases whose treatment is often a challenge. Many patients, even after immunosuppressive therapy, do not respond to treatment, so new alternatives have been sought for this. Therefore, other signaling pathways that could contribute to the pathogenesis of myositis have been investigated, such as the expression of myokines in skeletal muscle in response to the inflammatory process. In this review, we will refer to these muscle cytokines that are overexpressed or down...
Source: Journal of Immunology Research - August 13, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Gastrointestinal Motility and Improvement Efficacy of Shenhuang Plaster Application on Shenque: Identification, Evaluation, and Mechanism.
In this study, we elucidated the ingredients in SHP and evaluated its effects on PTX-induced constipation using a tumour-bearing mouse model. SHP was prepared using the traditional Chinese plaster preparation method. The ingredients were analysed using UPLC-MS/MS and identified via screening in a standard drug database. The gastrointestinal transit was evaluated by the movement of a fluorescein-labelled dextran in the gastrointestinal tract. A histological study of the mucosa was carried out after haematoxylin and eosin staining. mRNA expression was assessed using real-time RT-PCR, and the foetal microbiota composition was...
Source: Journal of Immunology Research - August 2, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Integrated Bioinformatics Analysis Identifies ELAVL1 and APP as Candidate Crucial Genes for Crohn's Disease.
In this study, three independent microarray studies of CD were integrated and 9912 differentially expressed genes (DEGs) were analysed by NetworkAnalyst to screen candidate crucial genes. NetworkAnalyst identified ELAV-like RNA binding protein 1 (ELAVL1) as the most crucial upregulated gene and amyloid-β precursor protein (APP) as the most crucial downregulated gene in peripheral blood of CD patients. By computing significance with hypergeometric test based on the KEGG pathway database, upregulated DEGs highlight the pathways of T cell receptor signaling and the differentiation of T helpers. Downregulated DEGs were fo...
Source: Journal of Immunology Research - July 31, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Linking Complement C3 and B Cells in Nasal Polyposis.
Authors: Werner U, Künstner A, Drenckhan M, Pries R, Bruchhage KL, Busch HS, Bachert C, Wollenberg B Abstract Nasal polyposis often is characterized by a persistent inflammation of the sinonasal mucosa, disease recurrence after medical or surgical intervention, and asthma comorbidity. Dysregulated complement activation may contribute to immunologic alterations and disease. To date, there is only scattered knowledge on the source and regulation of the central complement factors in the pathogenesis of nasal polyps. Here, we aim to study complement signatures, especially the C3-C3aR axis, and focus on cellular so...
Source: Journal of Immunology Research - July 31, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Assessing the Short-Term Efficacy and Safety of Guselkumab for Moderate-to-Severe Plaque Psoriasis: Meta-Analysis of Randomized Controlled Trials.
Conclusion: The guselkumab had excellent efficacy and great safety in moderate-to-severe plaque psoriasis, but long-term safety remained to be determined. PMID: 32724829 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - July 31, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Clinical Characteristics of Peripheral Neuropathy in Eosinophilic Granulomatosis with Polyangiitis: A Retrospective Single-Center Study in China.
Conclusion: PN in EGPA frequently displays with symmetrical multiple peripheral neuropathy in China. Positive ANCA, arthritis or joint pain, and higher BVAS are the independent associated factors of PN in EGPA. Glucocorticoids with immunosuppressants are vital therapeutic strategy. PMID: 32714994 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - July 28, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Urinary System Manifestation of IgG4-Related Disease: Clinical, Laboratory, Radiological, and Pathological Spectra of a Chinese Single-Centre Study.
Conclusion: IgG4-RUD had diverse lesion types and distinctive manifestations. Radiological examinations were helpful for diagnosis and treatment evaluation. Patients showed good initial response to immunosuppressive treatment but relapses could occur at the maintenance period. PMID: 32714995 [PubMed - in process] (Source: Journal of Immunology Research)
Source: Journal of Immunology Research - July 28, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research

Protective Immunity against SARS Subunit Vaccine Candidates Based on Spike Protein: Lessons for Coronavirus Vaccine Development.
Authors: Khalaj-Hedayati A Abstract The recent outbreak of the novel coronavirus disease, COVID-19, has highlighted the threat that highly pathogenic coronaviruses have on global health security and the imminent need to design an effective vaccine for prevention purposes. Although several attempts have been made to develop vaccines against human coronavirus infections since the emergence of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) in 2003, there is no available licensed vaccine yet. A better understanding of previous coronavirus vaccine studies may help to design a vaccine for the newly emerged viru...
Source: Journal of Immunology Research - July 24, 2020 Category: Allergy & Immunology Tags: J Immunol Res Source Type: research