The oncolytic adenovirus Delta-24-RGD in combination with ONC201 induces a potent antitumor response in pediatric high-grade and diffuse midline glioma models
CONCLUSIONS: The Delta-24-RGD/ONC201 combination improved the efficacy compared to each agent alone in in vitro and in vivo models by potentiating nuclear DNA damage and in turn improving the antitumor (immune) response to each agent alone.PMID:38554031 | DOI:10.1093/neuonc/noae066 (Source: Ann Oncol)
Source: Ann Oncol - March 30, 2024 Category: Cancer & Oncology Authors: Daniel de la Nava Iker Ausejo-Mauleon Virginia Laspidea Marisol Gonzalez-Huarriz Andrea Lacalle Noelia Casares Marta Zalacain Luc ía Marrodan Marc Garc ía-Moure Maria C Ochoa Antonio Carlos Tallon-Cobos Reyes Hernandez-Osuna Javier Marco-Sanz Laasya Dha Source Type: research
GSE262192 PEGboIFN-lambda as a FMD preexposure prophylactic and vaccine adjuvant in cattle —ISG and adaptive immunity gene expression
Contributors : Sarah E Attreed ; Christina Silva ; Monica Rodriguez-Calzada ; Aishwarya Mogulothu ; Sophia Abbott ; Paul Azzinaro ; Peter Canning ; Lillian Skidmore ; Jay Nelson ; Nick Knudsen ; Gisselle N Medina ; Teresa de los Santos ; Fayna D íaz-San SegundoSeries Type : Expression profiling by RT-PCROrganism : Bos taurusFoot-and-mouth disease (FMD) is a vesicular disease of cloven-hoofed animals with devastating economic implications. The current FMD vaccine, routinely used in enzootic countries, requires at least 7 days to induce protection. However, FMD vaccination is typically not recommended for use in ...
Source: GEO: Gene Expression Omnibus - March 30, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by RT-PCR Bos taurus Source Type: research
Cancers, Vol. 16, Pages 1359: Induction of Immunological Antitumor Effects by the Combination of Adenovirus-Mediated Gene Transfer of B7-1 and Anti-Programmed Cell Death-1 Antibody in a Murine Squamous Cell Carcinoma Model
Conclusions: These results suggest that the B7-1 gene transfer may enhance the antitumor effect of anti-PD1 antibody against SCC. (Source: Cancers)
Source: Cancers - March 30, 2024 Category: Cancer & Oncology Authors: Makiko Hara Sumiyo Saburi Natsumi Uehara Takahiro Tsujikawa Mie Kubo Tatsuya Furukawa Masanori Teshima Hirotaka Shinomiya Shigeru Hirano Ken-ichi Nibu Tags: Article Source Type: research
Optimal Conditions for Adenoviral Transduction of Immature Dendritic Cells Without Affecting the Tolerogenic Activity of DC-Based Immunotherapy
J Virol Methods. 2024 Mar 27:114921. doi: 10.1016/j.jviromet.2024.114921. Online ahead of print.ABSTRACTDendritic cells (DCs) play a pivotal role in maintaining immune tolerance. Using recombinant adenovirus (rAd) to deliver vectors to immature dendritic cells (imDCs) is an important method for studying the tolerogenic function of DCs. We found that using RPMI medium and a higher MOI during transduction increased the expression of CD80, CD86, and MHC-II on the surface of imDCs. Our data reveal a significant increase in the secretion of the pro-inflammatory cytokine IL-6 in the group showing the most pronounced phenotypic c...
Source: Journal of Virological Methods - March 29, 2024 Category: Virology Authors: Qian Jian Zongli Fu Hanyu Wang Hanyuan Zhang Yi Ma Source Type: research
Optimal Conditions for Adenoviral Transduction of Immature Dendritic Cells Without Affecting the Tolerogenic Activity of DC-Based Immunotherapy
J Virol Methods. 2024 Mar 27:114921. doi: 10.1016/j.jviromet.2024.114921. Online ahead of print.ABSTRACTDendritic cells (DCs) play a pivotal role in maintaining immune tolerance. Using recombinant adenovirus (rAd) to deliver vectors to immature dendritic cells (imDCs) is an important method for studying the tolerogenic function of DCs. We found that using RPMI medium and a higher MOI during transduction increased the expression of CD80, CD86, and MHC-II on the surface of imDCs. Our data reveal a significant increase in the secretion of the pro-inflammatory cytokine IL-6 in the group showing the most pronounced phenotypic c...
Source: Journal of Virological Methods - March 29, 2024 Category: Virology Authors: Qian Jian Zongli Fu Hanyu Wang Hanyuan Zhang Yi Ma Source Type: research
Viruses, Vol. 16, Pages 533: Prevalence of Respiratory Viral Infections in Deceased Persons during the COVID-19 Pandemic Season 2021 & ndash;2022: A Population-Based Observational Study
Viruses, Vol. 16, Pages 533: Prevalence of Respiratory Viral Infections in Deceased Persons during the COVID-19 Pandemic Season 2021–2022: A Population-Based Observational Study
Viruses doi: 10.3390/v16040533
Authors:
Camino Trobajo-Sanmartín
Ana Navascués
Miguel Fernández-Huerta
Iván Martínez-Baz
Itziar Casado
Carmen Ezpeleta
Jesús Castilla
Although the omicron variant of SARS-CoV-2 circulated intensely during the 2021–2022 season, many patients with severe acute respiratory disease tested negative for COVID-19. The aim of this study was to assess the presence of different...
Source: Viruses - March 29, 2024 Category: Virology Authors: Camino Trobajo-Sanmart ín Ana Navascu és Miguel Fern ández-Huerta Iv án Martínez-Baz Itziar Casado Carmen Ezpeleta Jes ús Castilla Tags: Article Source Type: research
Viruses, Vol. 16, Pages 532: HepG2BD: A Novel and Versatile Cell Line with Inducible HDV Replication and Constitutive HBV Expression
k Labonté
Individuals chronically infected with hepatitis B virus (HBV) and hepatitis Delta virus (HDV) present an increased risk of developing cirrhosis and hepatocellular carcinoma in comparison to HBV mono-infected individuals. Although HDV only replicates in individuals coinfected or superinfected with HBV, there is currently no in vitro model that can stably express both viruses simultaneously, mimicking the chronic infections seen in HBV/HDV patients. Here, we present the HepG2BD cell line as a novel in vitro culture system for long-term replication of HBV and HDV. HepG2BD cells derive from HepG2.2.15 cells in w...
Source: Viruses - March 29, 2024 Category: Virology Authors: Matthieu Blanchet L éna Angelo Yasmine T étreault Marwa Khabir Camille Sureau Andrew Vaillant Patrick Labont é Tags: Article Source Type: research
Modulation of the RAC1/MAPK/ERK signalling pathway by farnesyl diphosphate synthase regulates granulosa cells proliferation in polycystic ovary syndrome
AbstractPolycystic ovary syndrome (PCOS) is a complex gynaecological endocrine disease that occurs in women of childbearing age. The pathogenesis of PCOS is still unclear and further exploration is needed. Here, proteomic analysis indicated that the expression of farnesyl diphosphate synthase (FDPS) protein in ovarian tissue of PCOS mice was significantly decreased. The purpose of this study is to investigate the relationship between potential biomarkers of PCOS and granulosa cells (GCs) function. The mechanisms by which FDPS affected the proliferation of granulosa cells were also explored both in vitro and in vivo. We fou...
Source: Human Cell - March 29, 2024 Category: Cytology Source Type: research
Safety, efficacy, and biological data of T cell-enabling oncolytic adenovirus TILT-123 in advanced solid cancers from the TUNIMO monotherapy phase I trial
CONCLUSIONS: TILT-123 was safe and able to produce anti-tumor effects in local and distant lesions in heavily pre-treated patients. Good tolerability of TILT-123 facilitates combination studies, several of which are ongoing (NCT04217473, NCT05271318, NCT05222932, NCT06125197).PMID:38546220 | DOI:10.1158/1078-0432.CCR-23-3874 (Source: Cell Research)
Source: Cell Research - March 28, 2024 Category: Cytology Authors: Santeri A Pakola Katriina J Peltola James H A Clubb Elise Jirovec Lyna Haybout Tatiana V Kudling Tuomo Alanko Riitta Korpisaari Susanna Juteau Marjut Jaakkola Jorma Sormunen Jukka Kemppainen Annabrita Hemmes Teijo Pellinen Mirte van der Heijden Dafne C A Source Type: research
Safety, efficacy, and biological data of T cell-enabling oncolytic adenovirus TILT-123 in advanced solid cancers from the TUNIMO monotherapy phase I trial
CONCLUSIONS: TILT-123 was safe and able to produce anti-tumor effects in local and distant lesions in heavily pre-treated patients. Good tolerability of TILT-123 facilitates combination studies, several of which are ongoing (NCT04217473, NCT05271318, NCT05222932, NCT06125197).PMID:38546220 | DOI:10.1158/1078-0432.CCR-23-3874 (Source: Clinical Cancer Research)
Source: Clinical Cancer Research - March 28, 2024 Category: Cancer & Oncology Authors: Santeri A Pakola Katriina J Peltola James H A Clubb Elise Jirovec Lyna Haybout Tatiana V Kudling Tuomo Alanko Riitta Korpisaari Susanna Juteau Marjut Jaakkola Jorma Sormunen Jukka Kemppainen Annabrita Hemmes Teijo Pellinen Mirte van der Heijden Dafne C A Source Type: research
Safety, efficacy, and biological data of T cell-enabling oncolytic adenovirus TILT-123 in advanced solid cancers from the TUNIMO monotherapy phase I trial
CONCLUSIONS: TILT-123 was safe and able to produce anti-tumor effects in local and distant lesions in heavily pre-treated patients. Good tolerability of TILT-123 facilitates combination studies, several of which are ongoing (NCT04217473, NCT05271318, NCT05222932, NCT06125197).PMID:38546220 | DOI:10.1158/1078-0432.CCR-23-3874 (Source: Cancer Control)
Source: Cancer Control - March 28, 2024 Category: Cancer & Oncology Authors: Santeri A Pakola Katriina J Peltola James H A Clubb Elise Jirovec Lyna Haybout Tatiana V Kudling Tuomo Alanko Riitta Korpisaari Susanna Juteau Marjut Jaakkola Jorma Sormunen Jukka Kemppainen Annabrita Hemmes Teijo Pellinen Mirte van der Heijden Dafne C A Source Type: research
Safety, efficacy, and biological data of T cell-enabling oncolytic adenovirus TILT-123 in advanced solid cancers from the TUNIMO monotherapy phase I trial
CONCLUSIONS: TILT-123 was safe and able to produce anti-tumor effects in local and distant lesions in heavily pre-treated patients. Good tolerability of TILT-123 facilitates combination studies, several of which are ongoing (NCT04217473, NCT05271318, NCT05222932, NCT06125197).PMID:38546220 | DOI:10.1158/1078-0432.CCR-23-3874 (Source: Clinical Cancer Research)
Source: Clinical Cancer Research - March 28, 2024 Category: Cancer & Oncology Authors: Santeri A Pakola Katriina J Peltola James H A Clubb Elise Jirovec Lyna Haybout Tatiana V Kudling Tuomo Alanko Riitta Korpisaari Susanna Juteau Marjut Jaakkola Jorma Sormunen Jukka Kemppainen Annabrita Hemmes Teijo Pellinen Mirte van der Heijden Dafne C A Source Type: research
Adeno ‐associated virus‐based approach for genetic modification of cardiac fibroblasts in adult rat hearts
AbstractCardiac fibroblasts (CFs) are an attractive target for reducing pathological cardiac remodeling, and understanding the underlying mechanisms of these processes is the key to develop successful therapies for treating the pressure-overloaded heart. CF-specific knockout (KO) mouse lines with a Cre recombinase under the control of human TCF21 (hTCF21) promoter and/or an adeno-associated virus serotype 9 (AAV9)-hTCF21 system provide a powerful tool for understanding CF biology in vivo. Although a variety of rat disease models are vital for the research of cardiac fibrosis similar to mouse models, there are few rat mod...
Source: Physiological Reports - March 28, 2024 Category: Physiology Authors: Bridget Nieto,
Michael W. Cypress,
Bong Sook Jhun,
Jin O ‐Uchi Tags: SHORT REPORT Source Type: research
Pre-Existing Immunity to a Nucleic Acid Contaminant-Derived Antigen Mediates Transaminitis and Resultant Diminished Transgene Expression in a Mouse Model of Hepatic Recombinant Adeno-Associated Virus-Mediated Gene Transfer
Human Gene Therapy, Ahead of Print. (Source: Human Gene Therapy)
Source: Human Gene Therapy - March 28, 2024 Category: Genetics & Stem Cells Authors: Mark A. Brimble Christopher L. Morton Stephen M. Winston Isaiah L. Reeves Yunyu Spence Pei-Hsin Cheng Junfang Zhou Amit C. Nathwani Paul G. Thomas Aisha Souquette Andrew M. Davidoff Source Type: research
Long Non-Coding RNA MALAT1 Protects Against Spinal Cord Injury via Suppressing microRNA-125b-5p Mediated Microglial M1 Polarization, Neuroinflammation, and Neural Apoptosis
This study aimed to further explore the relationship between lncRNA MALAT1 and miR-125b-5p, as well as their effect on microglial activation, neuroinflammation, and neural apoptosis in spinal cord injury (SCI). Primary microglia from Sprague Dawley rats were stimulated with lipopolysaccharide (LPS). Then, microglia were transfected with lncRNA MALAT1 overexpression or knock-down adenovirus-associated virus with or without miR-125b-5p mimic. The culture medium of microglia was incubated with primary neuro ns. SCI rats were established for in vivo validation. LncRNA MALAT1 expression was reduced by LPS treatment in a dose...
Source: Molecular Neurobiology - March 27, 2024 Category: Neurology Source Type: research
