Targeting Mitochondrial Complex I Deficiency in MPP < sup > + < /sup > /MPTP-induced Parkinson's Disease Cell Culture and Mouse Models by Transducing Yeast NDI1 Gene
CONCLUSIONS: NDI1 compensates for the defective complex I in MPP+/MPTP-induced models, and vastly alleviates MPTP-induced toxic effect on dopaminergic neurons. Our study may provide a basis for gene therapy of sporadic PD with defective complex I caused by MPTP-like substance.PMID:38594619 | PMC:PMC11003148 | DOI:10.1186/s12575-024-00236-3 (Source: Biological Procedures Online)
Source: Biological Procedures Online - April 9, 2024 Category: Molecular Biology Authors: Hongzhi Li Jing Zhang Yuqi Shen Yifan Ye Qingyou Jiang Lan Chen Bohao Sun Zhuo Chen Luxi Shen Hezhi Fang Jifeng Yang Haihua Gu Source Type: research
Targeting Mitochondrial Complex I Deficiency in MPP < sup > + < /sup > /MPTP-induced Parkinson's Disease Cell Culture and Mouse Models by Transducing Yeast NDI1 Gene
CONCLUSIONS: NDI1 compensates for the defective complex I in MPP+/MPTP-induced models, and vastly alleviates MPTP-induced toxic effect on dopaminergic neurons. Our study may provide a basis for gene therapy of sporadic PD with defective complex I caused by MPTP-like substance.PMID:38594619 | PMC:PMC11003148 | DOI:10.1186/s12575-024-00236-3 (Source: Biological Procedures Online)
Source: Biological Procedures Online - April 9, 2024 Category: Molecular Biology Authors: Hongzhi Li Jing Zhang Yuqi Shen Yifan Ye Qingyou Jiang Lan Chen Bohao Sun Zhuo Chen Luxi Shen Hezhi Fang Jifeng Yang Haihua Gu Source Type: research
Targeting Mitochondrial Complex I Deficiency in MPP < sup > + < /sup > /MPTP-induced Parkinson's Disease Cell Culture and Mouse Models by Transducing Yeast NDI1 Gene
CONCLUSIONS: NDI1 compensates for the defective complex I in MPP+/MPTP-induced models, and vastly alleviates MPTP-induced toxic effect on dopaminergic neurons. Our study may provide a basis for gene therapy of sporadic PD with defective complex I caused by MPTP-like substance.PMID:38594619 | PMC:PMC11003148 | DOI:10.1186/s12575-024-00236-3 (Source: Biological Procedures Online)
Source: Biological Procedures Online - April 9, 2024 Category: Molecular Biology Authors: Hongzhi Li Jing Zhang Yuqi Shen Yifan Ye Qingyou Jiang Lan Chen Bohao Sun Zhuo Chen Luxi Shen Hezhi Fang Jifeng Yang Haihua Gu Source Type: research
Targeting Mitochondrial Complex I Deficiency in MPP < sup > + < /sup > /MPTP-induced Parkinson's Disease Cell Culture and Mouse Models by Transducing Yeast NDI1 Gene
CONCLUSIONS: NDI1 compensates for the defective complex I in MPP+/MPTP-induced models, and vastly alleviates MPTP-induced toxic effect on dopaminergic neurons. Our study may provide a basis for gene therapy of sporadic PD with defective complex I caused by MPTP-like substance.PMID:38594619 | PMC:PMC11003148 | DOI:10.1186/s12575-024-00236-3 (Source: Biological Procedures Online)
Source: Biological Procedures Online - April 9, 2024 Category: Molecular Biology Authors: Hongzhi Li Jing Zhang Yuqi Shen Yifan Ye Qingyou Jiang Lan Chen Bohao Sun Zhuo Chen Luxi Shen Hezhi Fang Jifeng Yang Haihua Gu Source Type: research
Targeting Mitochondrial Complex I Deficiency in MPP < sup > + < /sup > /MPTP-induced Parkinson's Disease Cell Culture and Mouse Models by Transducing Yeast NDI1 Gene
CONCLUSIONS: NDI1 compensates for the defective complex I in MPP+/MPTP-induced models, and vastly alleviates MPTP-induced toxic effect on dopaminergic neurons. Our study may provide a basis for gene therapy of sporadic PD with defective complex I caused by MPTP-like substance.PMID:38594619 | PMC:PMC11003148 | DOI:10.1186/s12575-024-00236-3 (Source: Biological Procedures Online)
Source: Biological Procedures Online - April 9, 2024 Category: Molecular Biology Authors: Hongzhi Li Jing Zhang Yuqi Shen Yifan Ye Qingyou Jiang Lan Chen Bohao Sun Zhuo Chen Luxi Shen Hezhi Fang Jifeng Yang Haihua Gu Source Type: research
Targeting Mitochondrial Complex I Deficiency in MPP < sup > + < /sup > /MPTP-induced Parkinson's Disease Cell Culture and Mouse Models by Transducing Yeast NDI1 Gene
CONCLUSIONS: NDI1 compensates for the defective complex I in MPP+/MPTP-induced models, and vastly alleviates MPTP-induced toxic effect on dopaminergic neurons. Our study may provide a basis for gene therapy of sporadic PD with defective complex I caused by MPTP-like substance.PMID:38594619 | PMC:PMC11003148 | DOI:10.1186/s12575-024-00236-3 (Source: Biological Procedures Online)
Source: Biological Procedures Online - April 9, 2024 Category: Molecular Biology Authors: Hongzhi Li Jing Zhang Yuqi Shen Yifan Ye Qingyou Jiang Lan Chen Bohao Sun Zhuo Chen Luxi Shen Hezhi Fang Jifeng Yang Haihua Gu Source Type: research
A GITRL ‐mTORC1‐GM‐CSF positive loop promotes pathogenic Th17 response in exacerbating Primary Sjögren's Syndrome
ConclusionsOur results identified the pathogenic role of GITRL in exacerbating disease activity and promoting pathogenic Th17 response in pSS through a GITRL/mTORC1/GM-CSF positive loop. These findings suggest GITRL might be a promising therapeutic target in the treatment of pSS. (Source: Arthritis and Rheumatology)
Source: Arthritis and Rheumatology - April 9, 2024 Category: Rheumatology Authors: Yuzhou Gan,
Haotian Zhou,
Yixue Guo,
Bo Huang,
Hongjiang Liu,
Ziye Wang,
Zijun Li,
Xiaozhen Zhao,
Huaqun Zhu,
Qimao Han,
Hua Ye,
Jing He,
Qingwen Wang,
Zhanguo Li,
Xiaolin Sun Tags: Full Length Source Type: research
Hemojuvelin mediated hepcidin induction requires both bone morphogenetic protein type I receptors ALK2 and ALK3
Blood Adv. 2024 Apr 8:bloodadvances.2023012322. doi: 10.1182/bloodadvances.2023012322. Online ahead of print.ABSTRACTHemojuvelin (HJV) is a GPI-anchored protein of the repulsive guidance molecule (RGM) family acting as a bone morphogenetic protein (BMP) co-receptor to induce the hepatic iron regulatory protein hepcidin. Hepcidin causes ubiquitination and degradation of the sole known iron exporter ferroportin thereby limiting iron availability. The detailed signaling mechanism of HJV in vivo has yet to be investigated. In the current manuscript, we used an established model of adeno-associated virus (AAV) mediated liver-sp...
Source: Adv Data - April 8, 2024 Category: Epidemiology Authors: Deniz Yildirim Dogan Eugen Ioan Urzica Isabelle Hornung Philipp Kastl David Oguama Franca Melanie Fette Lien Huong Nguyen Frank Rosenbauer Kai Zacharowski Ursula Klingm üller Elise Gradhand Andreas von Knethen R üdiger Popp Ingrid Fleming Lisa Schrader Source Type: research
Asparagine Synthetase Marks a Distinct Dependency Threshold for Cardiomyocyte Dedifferentiation
CONCLUSIONS: We discovered a novel gene Asns as both a molecular marker and an essential mediator, marking a distinct threshold that appears in common for at least 4 models of CMDD, and revealing an Asns/mammalian target of rapamycin complex 1 axis dependency for dedifferentiating cardiomyocytes. Further study will be needed to extrapolate and assess its relevance to other cell state transitions as well as in heart regeneration.PMID:38586957 | DOI:10.1161/CIRCULATIONAHA.123.063965 (Source: Circulation)
Source: Circulation - April 8, 2024 Category: Cardiology Authors: Yike Zhu Matthew Ackers-Johnson Muthu K Shanmugam Leroy Sivappiragasam Pakkiri Chester Lee Drum Yanpu Chen Johnny Kim Wyatt G Paltzer Ahmed I Mahmoud Wilson Lek Wen Tan Mick Chang Jie Lee Jianming Jiang Danh Anh Tuan Luu Shi Ling Ng Peter Yi Qing Li Anhui Source Type: research
Hemojuvelin mediated hepcidin induction requires both bone morphogenetic protein type I receptors ALK2 and ALK3
Blood Adv. 2024 Apr 8:bloodadvances.2023012322. doi: 10.1182/bloodadvances.2023012322. Online ahead of print.ABSTRACTHemojuvelin (HJV) is a GPI-anchored protein of the repulsive guidance molecule (RGM) family acting as a bone morphogenetic protein (BMP) co-receptor to induce the hepatic iron regulatory protein hepcidin. Hepcidin causes ubiquitination and degradation of the sole known iron exporter ferroportin thereby limiting iron availability. The detailed signaling mechanism of HJV in vivo has yet to be investigated. In the current manuscript, we used an established model of adeno-associated virus (AAV) mediated liver-sp...
Source: Adv Data - April 8, 2024 Category: Epidemiology Authors: Deniz Yildirim Dogan Eugen Ioan Urzica Isabelle Hornung Philipp Kastl David Oguama Franca Melanie Fette Lien Huong Nguyen Frank Rosenbauer Kai Zacharowski Ursula Klingm üller Elise Gradhand Andreas von Knethen R üdiger Popp Ingrid Fleming Lisa Schrader Source Type: research
Asparagine Synthetase Marks a Distinct Dependency Threshold for Cardiomyocyte Dedifferentiation
CONCLUSIONS: We discovered a novel gene Asns as both a molecular marker and an essential mediator, marking a distinct threshold that appears in common for at least 4 models of CMDD, and revealing an Asns/mammalian target of rapamycin complex 1 axis dependency for dedifferentiating cardiomyocytes. Further study will be needed to extrapolate and assess its relevance to other cell state transitions as well as in heart regeneration.PMID:38586957 | DOI:10.1161/CIRCULATIONAHA.123.063965 (Source: Circulation)
Source: Circulation - April 8, 2024 Category: Cardiology Authors: Yike Zhu Matthew Ackers-Johnson Muthu K Shanmugam Leroy Sivappiragasam Pakkiri Chester Lee Drum Yanpu Chen Johnny Kim Wyatt G Paltzer Ahmed I Mahmoud Wilson Lek Wen Tan Mick Chang Jie Lee Jianming Jiang Danh Anh Tuan Luu Shi Ling Ng Peter Yi Qing Li Anhui Source Type: research
Therapeutic prime/pull vaccination of HSV-2-infected guinea pigs with the ribonucleotide reductase 2 (RR2) protein and CXCL11 chemokine boosts antiviral local tissue-resident and effector memory CD4(+) and CD8(+) T cells and protects against recurrent genital herpes
In this study, we used the strategy that involves immunization of herpes simplex virus type 2-infected guinea pigs using a recombinantly expressed herpes tegument protein-ribonucleotide reductase 2 (RR2; prime), followed by intravaginal treatment with the neurotropic adeno-associated virus type 8 expressing CXCL9, CXCL10, or CXCL11 T-cell-attracting chemokines to recruit T cells into the infected dorsal root ganglia (DRG) and vaginal mucosa (VM) (pull). We show that the RR2/CXCL11 prime-pull therapeutic vaccine strategy elicited a significant reduction in virus shedding in the vaginal mucosa and decreased the severity and ...
Source: Herpes - April 8, 2024 Category: Infectious Diseases Authors: Afshana Quadiri Swayam Prakash Nisha Rajeswari Dhanushkodi Mahmoud Singer Latifa Zayou Amin Mohammed Shaik Miyo Sun Berfin Suzer Lauren Su Lin Lau Amruth Chilukurri Hawa Vahed Hubert Schaefer Lbachir BenMohamed Source Type: research
Genes, Vol. 15, Pages 467: Redirect Tropism of Fowl Adenovirus 4 Vector by Modifying Fiber2 with Variable Domain of Heavy-Chain Antibody
Zhuozhuang Lu
The variable domain of a heavy-chain antibody (VHH) has the potential to be used to redirect the cell tropism of adenoviral vectors. Here, we attempted to establish platforms to simplify the screening of VHHs for their specific targeting function when being incorporated into the fiber of adenovirus. Both fowl adenovirus 4 (FAdV-4) and simian adenovirus 1 (SAdV-1) have two types of fiber, one of which is dispensable for virus propagation and is a proper site for VHH display. An intermediate plasmid, pMD-FAV4Fs, was constructed as the start plasmid for FAdV-4 fiber2 modification. Foldon from phage T4 fibrit...
Source: Genes - April 8, 2024 Category: Genetics & Stem Cells Authors: Yongjin Wang Xiaohui Zou Xiaojuan Guo Zhichao Zhang Min Wang Tao Hung Zhuozhuang Lu Tags: Article Source Type: research
Corrigendum to "Enhanced cancer cell killing by truncated E2F-1 used in combination with oncolytic adenovirus" [Virology 433 (2) (2012) 538-547]
Virology. 2024 Apr 6;595:110079. doi: 10.1016/j.virol.2024.110079. Online ahead of print.NO ABSTRACTPMID:38583304 | DOI:10.1016/j.virol.2024.110079 (Source: Virology)
Source: Virology - April 7, 2024 Category: Virology Authors: Jorge G Gomez-Gutierrez Xia-Mei Rao Heshan Sam Zhou Kelly M McMasters Source Type: research
