Imaging of adeno-associated viral capsids for purposes of gene editing using CEST NMR/MRI
CONCLUSION: AAV2 viral capsids exhibit strong capacity as an endogenous CEST contrast agent and can potentially be used for monitoring and evaluation of AAV vector-mediated gene therapy protocols.PMID:38651648 | DOI:10.1002/mrm.30058 (Source: Magnetic Resonance in Medicine)
Source: Magnetic Resonance in Medicine - April 23, 2024 Category: Radiology Authors: Bonnie Lam Mark Velasquez Tomoko Ogiyama Kevin Godines Fan-Yun Szu A J Velasquez-Mao Wissam AlGhuraibawi Jingshen Wang Phillip B Messersmith Moriel H Vandsburger Source Type: research
Viruses, Vol. 16, Pages 658: Expanding the Scope of Adenoviral Vectors by Utilizing Novel Tools for Recombination and Vector Rescue
Ruzsics
Recombinant adenoviruses are widely used in clinical and laboratory applications. Despite the wide variety of available sero- and genotypes, only a fraction is utilized in vivo. As adenoviruses are a large group of viruses, displaying many different tropisms, immune epitopes, and replication characteristics, the merits of translating these natural benefits into vector applications are apparent. This translation, however, proves difficult, since while research has investigated the application of these viruses, there are no universally applicable rules in vector design for non-classical adenovirus types. In t...
Source: Viruses - April 23, 2024 Category: Virology Authors: Fischer Fedotova B ühler Darriba Schreiner Ruzsics Tags: Article Source Type: research
Immune reconstitution in children after haploidentical haematopoietic stem cell transplantation
CONCLUSION: Paediatric patients who undergo haploidentical HSCT with PTCy are likely to have delayed IR and an increased risk of viral reactivation/infection compared with HLA-matched HSCT. The addition of ATG to PTCy delayed T cell recovery and increased risk of BKV reactivation.PMID:38646695 | DOI:10.1111/ijlh.14290 (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - April 22, 2024 Category: Hematology Authors: Saranthorn Apasuthirat Nopporn Apiwattanakul Usanarat Anurathapan Nintita Sripaiboonkij Thokanit Karan Paisooksantivatana Ekawat Pasomsub Suradej Hongeng Samart Pakakasama Source Type: research
Impact of the coronavirus disease 2019 pandemic on the incidence of other infectious diseases in the hematology hospital in Korea
CONCLUSIONS: The implementation of COVID-19 infection control measures reduced the incidence of cRV infection. However, CDI increased significantly and incidence rates of CPE and VRE remained unchanged in hematological patients after the pandemic. Infection control measures suitable for each type of HCAI, such as stringent hand washing for CDI and enough isolation capacities, should be implemented and maintained in future pandemics, especially in immunocompromised patients.PMID:38649159 | DOI:10.3904/kjim.2023.508 (Source: The Korean Journal of Internal Medicine)
Source: The Korean Journal of Internal Medicine - April 22, 2024 Category: Internal Medicine Authors: Seohee Oh Yu-Sun Sung Mihee Jang Yong-Jin Kim Hyun-Wook Park Dukhee Nho Dong-Gun Lee Hyeon Woo Yim Sung-Yeon Cho Source Type: research
Correction: Long-term antigen-specific immune response by an oncolytic adenovirus encoding SP-SA-E7-4-1BBL in HPV-16 cancer model
Mol Biol Rep. 2024 Apr 22;51(1):565. doi: 10.1007/s11033-024-09489-3.NO ABSTRACTPMID:38649646 | DOI:10.1007/s11033-024-09489-3 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - April 22, 2024 Category: Molecular Biology Authors: Alejandra G Martinez-Perez Rodolfo Garza-Morales Maria J de Loera-Arias Sheila A Villa-Cedillo Aracely Garcia-Garcia Humberto Rodriguez-Rocha Orlando E Flores-Maldonado Jesus Valdes Jose J Perez-Trujillo Odila Saucedo-Cardenas Source Type: research
Unlocking the potential of adeno-associated virus in neuroscience: a brief review
Mol Biol Rep. 2024 Apr 22;51(1):563. doi: 10.1007/s11033-024-09521-6.ABSTRACTAdeno-associated virus (AAV) has emerged as a pivotal tool in neuroscience research, owing to its remarkable versatility and efficiency in delivering genetic material to diverse cell types within the nervous system. This mini review aims to underscore the advanced applications of AAV vectors in neuroscience and their profound potential to revolutionize our understanding of brain function and therapeutic interventions for neurological disorders. By providing a concise overview of the latest developments and strategies employing AAV vectors, this re...
Source: Molecular Biology Reports - April 22, 2024 Category: Molecular Biology Authors: Antea Minetti Source Type: research
Characterization and optimization of exopolysaccharide extracted from a newly isolated halotolerant cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1 with antiviral activity
This study aimed to identify and optimize the conditions for exopolysaccharide (EPS) production from a newly isolated cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1, besides exploring its antiviral activity. The cyanobacterial EPS was purified through DEAE-52 cellulose column with a final yield of 83.75%. Different analysis instruments were applied for EPS identification, including Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), and gas chromatographic-mass spectrometry (GC-MS). Plackett-Burman's design demonstrated that working volume (X1), EDTA (X2), inoculum size (X3), CaCl2 (X4...
Source: Herpes - April 21, 2024 Category: Infectious Diseases Authors: Mabroka H Saad Nagwa M Sidkey Esmail M El-Fakharany Source Type: research
Efficacy and Safety of Recombinant Human p53 Adenovirus Injection Combined with Radiotherapy or Concurrent Chemoradiotherapy in the Treatment of Cervical Cancer: A Systematic Review and Meta-analysis
CONCLUSIONS: The combination of rAd-p53 with radiotherapy or concurrent chemoradiotherapy exhibits enhanced therapeutic efficacy in the treatment of cervical cancer without a significant increase in severe adverse reactions. However, multicenter, large-scale, randomized clinical trials are warranted for further validation.PMID:38639607 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - April 19, 2024 Category: Complementary Medicine Authors: Guangshu Zhang Guizhen Su Source Type: research
Efficacy and Safety of Recombinant Human p53 Adenovirus Injection Combined with Radiotherapy or Concurrent Chemoradiotherapy in the Treatment of Cervical Cancer: A Systematic Review and Meta-analysis
CONCLUSIONS: The combination of rAd-p53 with radiotherapy or concurrent chemoradiotherapy exhibits enhanced therapeutic efficacy in the treatment of cervical cancer without a significant increase in severe adverse reactions. However, multicenter, large-scale, randomized clinical trials are warranted for further validation.PMID:38639607 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - April 19, 2024 Category: Complementary Medicine Authors: Guangshu Zhang Guizhen Su Source Type: research
Activation of Wnt signaling mitigates blood-brain barrier disruption by inhibiting vesicular transcytosis after traumatic brain injury in mice
This study aims to elucidate the impact of Wnt/β-catenin signaling on cerebrovascular vesicular transcytosis following TBI. In this experiment, adult male wild-type (WT) C57BL/6 mice underwent various interventions. TBI was induced utilizing the controlled cortical impact technique. Post-TBI, mice were administered either an inhibitor or an agonist of Wnt signaling via intraperitoneal injection. Recombinant adeno-associated virus (rAAV) was administered intracerebroventricularly to modulate the expression of the CAV-1 inhibitory protein, Major facilitator superfamily domain-containing 2a (Mfsd2a). This research utilized E...
Source: Experimental Neurology - April 19, 2024 Category: Neurology Authors: Yuan Zhang Jianfeng Xu Pengcheng Li Bo Luo Hui Tang Source Type: research
Viruses, Vol. 16, Pages 633: DMSO and Its Role in Differentiation Impact Efficacy of Human Adenovirus (HAdV) Infection in HepaRG Cells
Schreiner
Differentiated HepaRG cells are popular in vitro cell models for hepatotoxicity studies. Their differentiation is usually supported by the addition of dimethyl sulfoxide (DMSO), an amphipathic solvent widely used in biomedicine, for example, in potential novel therapeutic drugs and cryopreservation of oocytes. Recent studies have demonstrated drastic effects, especially on epigenetics and extracellular matrix composition, induced by DMSO, making its postulated inert character doubtful. In this work, the influence of DMSO and DMSO-mediated modulation of differentiation on human adenovirus (HAdV) infection of H...
Source: Viruses - April 19, 2024 Category: Virology Authors: Katharina Hofmann Samuel Hofmann Franziska Weigl Julia Mai Sabrina Schreiner Tags: Article Source Type: research
Isthmin-1 alleviates cardiac ischemia/reperfusion injury through cGMP-PKG signaling pathway
CONCLUSION: ISM1 can protect against cardiac I/R injury through cGMP-PKG signaling pathway, and it is a promising therapeutic and predictive target of cardiac I/R injury.PMID:38637328 | DOI:10.1093/cvr/cvae077 (Source: Cell Research)
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Min Hu Xin Zhang Can Hu Zhen-Guo Ma Sha-Sha Wang Teng Teng Xiao-Feng Zeng Qi-Zhu Tang Source Type: research
Effects of Interleukin-19 overexpression in the medial prefrontal cortex on anxiety-related behaviors, BDNF expression and p38/JNK/ERK pathways
In this study, we initiated an acute restraint stress (ARS)-induced anxious mouse model and identified heightened expression of IL-19 and IL-20Rα in the medial prefrontal cortex (mPFC) of ARS mice. Notably, IL-19 and IL-20Rα were predominantly present in the excitatory pyramidal neurons of the mPFC under both basal and ARS conditions. Utilizing the adeno-associated virus (AAV) strategy, we demonstrated that IL-19 overexpression in the mPFC induced anxiety-related behaviors and elevated stress susceptibility. Additionally, we observed decreased protein levels of brain-derived neurotrophic factor (BDNF) and postsynaptic de...
Source: Brain Research Bulletin - April 18, 2024 Category: Neurology Authors: Yuting Jiang Lihong Xu Yifan Cao Fantao Meng Shujun Jiang Mengyu Yang Ziteng Zheng Yi Zhang Lu Yang Meiqin Wang Guizhi Sun Jing Liu Chen Li Minghu Cui Source Type: research
Effects of Interleukin-19 overexpression in the medial prefrontal cortex on anxiety-related behaviors, BDNF expression and p38/JNK/ERK pathways
In this study, we initiated an acute restraint stress (ARS)-induced anxious mouse model and identified heightened expression of IL-19 and IL-20Rα in the medial prefrontal cortex (mPFC) of ARS mice. Notably, IL-19 and IL-20Rα were predominantly present in the excitatory pyramidal neurons of the mPFC under both basal and ARS conditions. Utilizing the adeno-associated virus (AAV) strategy, we demonstrated that IL-19 overexpression in the mPFC induced anxiety-related behaviors and elevated stress susceptibility. Additionally, we observed decreased protein levels of brain-derived neurotrophic factor (BDNF) and postsynaptic de...
Source: Brain Research - April 18, 2024 Category: Neurology Authors: Yuting Jiang Lihong Xu Yifan Cao Fantao Meng Shujun Jiang Mengyu Yang Ziteng Zheng Yi Zhang Lu Yang Meiqin Wang Guizhi Sun Jing Liu Chen Li Minghu Cui Source Type: research
TRPC4 aggravates hypoxic pulmonary hypertension by promoting pulmonary endothelial cell apoptosis
In this study, we aimed to investigate whether TRPC4 in PAECs has an aggravating effect on PH and elucidate the molecular mechanisms. We observed that hypoxia treatment promoted PAEC apoptosis through a caspase-12/endoplasmic reticulum stress (ERS)-dependent pathway. Knockdown of TRPC4 attenuated hypoxia-induced apoptosis and caspase-3/caspase-12 activity in PAECs. Accordingly, adeno-associated virus (AAV) serotype 6-mediated pulmonary endothelial TRPC4 silencing (AAV6-Tie-shRNA-TRPC4) or TRPC4 antagonist suppressed PH progression as evidenced by reduced right ventricular systolic pressure (RVSP), pulmonary vascular remode...
Source: Free Radical Biology and Medicine - April 18, 2024 Category: Biology Authors: Liu Yang Zeyu Peng Fanpeng Gong WenXin Yan Yi Shi Hanyi Li Chang Zhou Hong Yao Menglu Yuan Fan Yu Lei Feng Naifu Wan Guizhu Liu Source Type: research
