Pre-Existing Immunity to a Nucleic Acid Contaminant-Derived Antigen Mediates Transaminitis and Resultant Diminished Transgene Expression in a Mouse Model of Hepatic Recombinant Adeno-Associated Virus-Mediated Gene Transfer
Human Gene Therapy, Ahead of Print. (Source: Human Gene Therapy)
Source: Human Gene Therapy - March 28, 2024 Category: Genetics & Stem Cells Authors: Mark A. Brimble Christopher L. Morton Stephen M. Winston Isaiah L. Reeves Yunyu Spence Pei-Hsin Cheng Junfang Zhou Amit C. Nathwani Paul G. Thomas Aisha Souquette Andrew M. Davidoff Source Type: research
Long Non-Coding RNA MALAT1 Protects Against Spinal Cord Injury via Suppressing microRNA-125b-5p Mediated Microglial M1 Polarization, Neuroinflammation, and Neural Apoptosis
This study aimed to further explore the relationship between lncRNA MALAT1 and miR-125b-5p, as well as their effect on microglial activation, neuroinflammation, and neural apoptosis in spinal cord injury (SCI). Primary microglia from Sprague Dawley rats were stimulated with lipopolysaccharide (LPS). Then, microglia were transfected with lncRNA MALAT1 overexpression or knock-down adenovirus-associated virus with or without miR-125b-5p mimic. The culture medium of microglia was incubated with primary neuro ns. SCI rats were established for in vivo validation. LncRNA MALAT1 expression was reduced by LPS treatment in a dose...
Source: Molecular Neurobiology - March 27, 2024 Category: Neurology Source Type: research
Evaluation of Mastadenovirus and Rotavirus Presence in Phyllostomid, Vespertilionid, and Molossid Bats Captured in Rio Grande do Sul, Southern Brazil
Food Environ Virol. 2024 Mar 26. doi: 10.1007/s12560-023-09575-y. Online ahead of print.ABSTRACTBat-borne viruses may affect public health and the global economy. These mammals have a wide geographical distribution and unique biological, physiological, and immunogenic characteristics, allowing the dissemination of many known and unknown viruses. Enteric viruses, such as adeno (AdV) and rotaviruses, are recognized as the main causative agents of disease and outbreaks. In the present study, the presence of viruses from Adenoviridae and Reoviridae families was evaluated in molossid, phyllostomid, and vespertilionid bats captu...
Source: Adv Data - March 27, 2024 Category: Epidemiology Authors: Alexandre Sita Gabriela Esp índola Birlem Deivid de Souza da Silva Gabriela Mattos Possamai Karla Petry Paula Rodrigues de Almeida Larissa Mallmann Jana ína Franciele Stein Meriane Demoliner Juliana Schons Gularte Alana Witt Hansen Andr é Alberto Witt Source Type: research
A Potential Role of CD82/KAI1 during Uterine Decidualization in Mice
Curr Issues Mol Biol. 2024 Feb 27;46(3):1799-1809. doi: 10.3390/cimb46030118.ABSTRACTThe tumor metastasis suppressor gene CD82/KAI1 has been demonstrated to impact human trophoblast invasion and migration. Communication between trophoblasts and decidual stromal cells plays a crucial role in controlling the normal invasiveness of trophoblasts. However, whether CD82/KAI1 is involved in decidualization and what role it plays remain unclear. CD82/KAI1 demonstrates specific spatiotemporal expression patterns in stromal cells undergoing decidualization during pregnancy. This is observed in both naturally pregnant females post-im...
Source: Mol Biol Cell - March 27, 2024 Category: Molecular Biology Authors: Qijun Li Mengyao Song Ke Cao Qian Zhang Source Type: research
Computationally guided AAV engineering for enhanced gene delivery
Trends Biochem Sci. 2024 Mar 25:S0968-0004(24)00054-9. doi: 10.1016/j.tibs.2024.03.002. Online ahead of print.ABSTRACTGene delivery vehicles based on adeno-associated viruses (AAVs) are enabling increasing success in human clinical trials, and they offer the promise of treating a broad spectrum of both genetic and non-genetic disorders. However, delivery efficiency and targeting must be improved to enable safe and effective therapies. In recent years, considerable effort has been invested in creating AAV variants with improved delivery, and computational approaches have been increasingly harnessed for AAV engineering. In t...
Source: Trends in Biochemical Sciences - March 26, 2024 Category: Biochemistry Authors: Jingxuan Guo Li F Lin Sydney V Oraskovich Julio A Rivera de Jes ús Jennifer Listgarten David V Schaffer Source Type: research
Harnessing adenovirus in cancer immunotherapy: evoking cellular immunity and targeting delivery in cell-specific manner
Biomark Res. 2024 Mar 25;12(1):36. doi: 10.1186/s40364-024-00581-1.ABSTRACTRecombinant adenovirus (rAd) regimens, including replication-competent oncolytic adenovirus (OAV) and replication-deficient adenovirus, have been identified as potential cancer therapeutics. OAV presents advantages such as selective replication, oncolytic efficacy, and tumor microenvironment (TME) remodeling. In this perspective, the principles and advancements in developing OAV toolkits are reviewed. The burgeoning rAd may dictate efficacy of conventional cancer therapies as well as cancer immunotherapies, including cancer vaccines, synergy with ad...
Source: Cell Research - March 26, 2024 Category: Cytology Authors: Miao Zeng Wei Zhang Yisheng Li Li Yu Source Type: research
Computationally guided AAV engineering for enhanced gene delivery
Trends Biochem Sci. 2024 Mar 25:S0968-0004(24)00054-9. doi: 10.1016/j.tibs.2024.03.002. Online ahead of print.ABSTRACTGene delivery vehicles based on adeno-associated viruses (AAVs) are enabling increasing success in human clinical trials, and they offer the promise of treating a broad spectrum of both genetic and non-genetic disorders. However, delivery efficiency and targeting must be improved to enable safe and effective therapies. In recent years, considerable effort has been invested in creating AAV variants with improved delivery, and computational approaches have been increasingly harnessed for AAV engineering. In t...
Source: Trends in Biochemical Sciences - March 26, 2024 Category: Biochemistry Authors: Jingxuan Guo Li F Lin Sydney V Oraskovich Julio A Rivera de Jes ús Jennifer Listgarten David V Schaffer Source Type: research
Viruses, Vol. 16, Pages 507: Disease Burden and Inpatient Management of Children with Acute Respiratory Viral Infections during the Pre-COVID Era in Germany: A Cost-of-Illness Study
rbara A. Rath
Respiratory viral infections (RVIs) are common reasons for healthcare consultations. The inpatient management of RVIs consumes significant resources. From 2009 to 2014, we assessed the costs of RVI management in 4776 hospitalized children aged 0–18 years participating in a quality improvement program, where all ILI patients underwent virologic testing at the National Reference Centre followed by detailed recording of their clinical course. The direct (medical or non-medical) and indirect costs of inpatient management outside the ICU (‘non-ICU’) versus management r...
Source: Viruses - March 26, 2024 Category: Virology Authors: Maren Alchikh Tim O. F. Conrad Patrick E. Obermeier Xiaolin Ma Brunhilde Schweiger Onya Opota Barbara A. Rath Tags: Article Source Type: research
Evaluation of Mastadenovirus and Rotavirus Presence in Phyllostomid, Vespertilionid, and Molossid Bats Captured in Rio Grande do Sul, Southern Brazil
AbstractBat-borne viruses may affect public health and the global economy. These mammals have a wide geographical distribution and unique biological, physiological, and immunogenic characteristics, allowing the dissemination of many known and unknown viruses. Enteric viruses, such as adeno (AdV) and rotaviruses, are recognized as the main causative agents of disease and outbreaks. In the present study, the presence of viruses fromAdenoviridae andReoviridae families was evaluated in molossid, phyllostomid, and vespertilionid bats captured in Rio Grande do Sul, Southern Brazil, between September 2021 and July 2022. Sixty bat...
Source: Food and Environmental Virology - March 26, 2024 Category: Virology Source Type: research
Deaths Associated with Pediatric Hepatitis of Unknown Etiology, United States, October 2021-June 2023
We describe demographic and clinical characteristics, including potential involvement of adenovirus in development of acute hepatitis, of 8 fatally ill children who met reporting criteria. The children had diverse courses of illness. Two children were immunocompromised when initially brought for care. Four children tested positive for adenovirus in multiple specimen types, including 2 for whom typing was completed. One adenovirus-positive child had no known underlying conditions, supporting a potential relationship between adenovirus and acute hepatitis in previously healthy children. Our findings emphasize the importance ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Olivia Almendares Julia M Baker David E Sugerman Umesh D Parashar Sarah Reagan-Steiner Hannah L Kirking Paul A Gasta ñaduy Jacqueline E Tate Hepatitis of Unknown Etiology Group2 Source Type: research
Deaths Associated with Pediatric Hepatitis of Unknown Etiology, United States, October 2021-June 2023
We describe demographic and clinical characteristics, including potential involvement of adenovirus in development of acute hepatitis, of 8 fatally ill children who met reporting criteria. The children had diverse courses of illness. Two children were immunocompromised when initially brought for care. Four children tested positive for adenovirus in multiple specimen types, including 2 for whom typing was completed. One adenovirus-positive child had no known underlying conditions, supporting a potential relationship between adenovirus and acute hepatitis in previously healthy children. Our findings emphasize the importance ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Olivia Almendares Julia M Baker David E Sugerman Umesh D Parashar Sarah Reagan-Steiner Hannah L Kirking Paul A Gasta ñaduy Jacqueline E Tate Hepatitis of Unknown Etiology Group2 Source Type: research
Deaths Associated with Pediatric Hepatitis of Unknown Etiology, United States, October 2021-June 2023
We describe demographic and clinical characteristics, including potential involvement of adenovirus in development of acute hepatitis, of 8 fatally ill children who met reporting criteria. The children had diverse courses of illness. Two children were immunocompromised when initially brought for care. Four children tested positive for adenovirus in multiple specimen types, including 2 for whom typing was completed. One adenovirus-positive child had no known underlying conditions, supporting a potential relationship between adenovirus and acute hepatitis in previously healthy children. Our findings emphasize the importance ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Olivia Almendares Julia M Baker David E Sugerman Umesh D Parashar Sarah Reagan-Steiner Hannah L Kirking Paul A Gasta ñaduy Jacqueline E Tate Hepatitis of Unknown Etiology Group2 Source Type: research
Deaths Associated with Pediatric Hepatitis of Unknown Etiology, United States, October 2021-June 2023
We describe demographic and clinical characteristics, including potential involvement of adenovirus in development of acute hepatitis, of 8 fatally ill children who met reporting criteria. The children had diverse courses of illness. Two children were immunocompromised when initially brought for care. Four children tested positive for adenovirus in multiple specimen types, including 2 for whom typing was completed. One adenovirus-positive child had no known underlying conditions, supporting a potential relationship between adenovirus and acute hepatitis in previously healthy children. Our findings emphasize the importance ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Olivia Almendares Julia M Baker David E Sugerman Umesh D Parashar Sarah Reagan-Steiner Hannah L Kirking Paul A Gasta ñaduy Jacqueline E Tate Hepatitis of Unknown Etiology Group2 Source Type: research
Deaths Associated with Pediatric Hepatitis of Unknown Etiology, United States, October 2021-June 2023
We describe demographic and clinical characteristics, including potential involvement of adenovirus in development of acute hepatitis, of 8 fatally ill children who met reporting criteria. The children had diverse courses of illness. Two children were immunocompromised when initially brought for care. Four children tested positive for adenovirus in multiple specimen types, including 2 for whom typing was completed. One adenovirus-positive child had no known underlying conditions, supporting a potential relationship between adenovirus and acute hepatitis in previously healthy children. Our findings emphasize the importance ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Olivia Almendares Julia M Baker David E Sugerman Umesh D Parashar Sarah Reagan-Steiner Hannah L Kirking Paul A Gasta ñaduy Jacqueline E Tate Hepatitis of Unknown Etiology Group2 Source Type: research
Deaths Associated with Pediatric Hepatitis of Unknown Etiology, United States, October 2021-June 2023
We describe demographic and clinical characteristics, including potential involvement of adenovirus in development of acute hepatitis, of 8 fatally ill children who met reporting criteria. The children had diverse courses of illness. Two children were immunocompromised when initially brought for care. Four children tested positive for adenovirus in multiple specimen types, including 2 for whom typing was completed. One adenovirus-positive child had no known underlying conditions, supporting a potential relationship between adenovirus and acute hepatitis in previously healthy children. Our findings emphasize the importance ...
Source: Emerging Infectious Diseases - March 25, 2024 Category: Infectious Diseases Authors: Olivia Almendares Julia M Baker David E Sugerman Umesh D Parashar Sarah Reagan-Steiner Hannah L Kirking Paul A Gasta ñaduy Jacqueline E Tate Hepatitis of Unknown Etiology Group2 Source Type: research
