Association of rs11549465 (C1772T) variant of hypoxia-inducible factor-1 α with Covid-19 susceptibility. A population-based epidemiological study
(Source: Human Cell)
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Upregulation of lncRNA NIFK-AS1 in hepatocellular carcinoma by m6A methylation promotes disease progression and sorafenib resistance
AbstractLong non-coding RNAs (LncRNAs) have recently emerged as vital regulators in the development and progression of hepatocellular carcinoma (HCC), providing new opportunities as novel therapeutic targets. Here we identified the lncRNA NIFK-AS1 as being highly expressed in HCC tissues and cells and showed this up-regulation resulted from METTL3-dependent m6A methylation. Functionally, knockdown of NIFK-AS1 inhibited the proliferation, colony formation, migration, and invasion of HCC cells. Moreover, these effects were elicited though AKT1 and we uncovered a ceRNA network involving an NIFK-AS1/miR-637/AKT1 axis with down...
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

STAT3 inhibitor BBI608 enhances the antitumor effect of gefitinib on EGFR-mutated non-small cell lung cancer cells
In conclusion, STAT3 inhibitor enhanced the antitumor effect of gefitinib on EGFR-mutated NSCLC cells through regulating ROR1/ABCB1/P53 pathway. (Source: Human Cell)
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Trelagliptin ameliorates oxygen –glucose deprivation/reperfusion (OGD/R)-induced mitochondrial dysfunction and metabolic disturbance of endothelial cells
AbstractAcute myocardial infarction (AMI) is a severe cardiovascular disease with high mortality. It is reported to be closely related to the mitochondrial dysfunction and metabolic disturbance on endothelial cells under a chronic hypoxic state. Significant declined mitochondrial respiration, ATP production, and metabolic changes are the main characteristics of endothelial injury in the disease. Trelagliptin is a DPP-4 inhibitor applied for the treatment of type II diabetes and has been recently reported to exert various pharmacological properties. In this investigation, we examined whether Trelagliptin possessed a protect...
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Effects of platinum-coexisting dopamine with X-ray irradiation upon human glioblastoma cell proliferation
AbstractIn brain tumors, neurotransmitters and platinum drugs may have some interaction, but their role in radiation therapy remains unclear. We investigated the effects of dopamine in combination with platinum on human glioblastoma U-251MG cells upon X-ray irradiation, comparing withl-DOPA, 2-phenylethylamine and temozolomide. Cell proliferation of U-251MG cells was prominently decreased by dopamine in combination with 10  μM platinum upon 4 Gy of X-ray irradiation, accompanied with intracellular reactive oxygen species generation and mitotic catastrophe. Platinum alone did not increase intracellular reactive...
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Establishment and characterization of NCC-MFS4-C1: a novel patient-derived cell line of myxofibrosarcoma
AbstractMyxofibrosarcoma (MFS) is an aggressive sarcoma with a highly complex karyotype. Complete resection is the only curative treatment for MFS because it is refractory to chemotherapy. To improve clinical outcomes, it is critical to develop novel treatments for MFS. Although patient-derived cell lines play a key role in cancer research, only 12 MFS cell lines have been reported to date, and considering the diversity of the disease, more cell lines need to be established. Hence, in the present study, we established a novel MFS cell line, NCC-MFS4-C1, using a surgically resected tumor tissue from a patient with MFS. NCC-...
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Neurological effects of elevated levels of angiotensin II in COVID-19 patients
(Source: Human Cell)
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

GATA3 as a regulator for naughty cancer-associated fibroblasts in the microenvironment of high-grade serous ovarian cancer
(Source: Human Cell)
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Association of ApoE isoforms with COVID-19 outcomes: a world-wide epidemiological study
(Source: Human Cell)
Source: Human Cell - October 4, 2021 Category: Cytology Source Type: research

Macrophage/microglia-derived IL-1 β induces glioblastoma growth via the STAT3/NF-κB pathway
AbstractGlioblastoma is a glioma characterized by highly malignant features. Numerous studies conducted on the relationship between glioblastoma and the microenvironment have indicated the significance of tumor-associated macrophages/microglia (TAMs) in glioblastoma progression. Since interleukin (IL)-1 β secreted by TAMs has been suggested to promote glioblastoma growth, we attempted to elucidate the detailed mechanisms of IL-1β in glioblastoma growth in this study. A phospho-receptor tyrosine kinase array and RNA-sequencing studies indicated that IL-1β induced the activation of signal transduc er and activ...
Source: Human Cell - September 30, 2021 Category: Cytology Source Type: research

Addition of ROCK inhibitors to prostaglandin derivative (PG) synergistically affects adipogenesis of the 3D spheroids of human orbital fibroblasts (HOFs)
AbstractTo study the additive effects of Rho-associated coiled-coil containing protein kinase inhibitors, ripasudil (Rip) to bimatoprost acid (BIM-A) on orbital adipose tissue, three-dimensional (3D) cultures of human orbital fibroblasts (HOFs) were prepared and the physical properties including the 3D spheroid size and stiffness, lipid staining by BODIPY and the mRNA expression of adipogenesis-related genes,PPAR γ andAP2, and extracellular matrix (ECM) including collagen (COL)1, 4 and 6, and fibronectin (FN) were analyzed. Adipogenesis (DIF+) induced (1) enlargement and increasing stiffness of the 3D HOFs spheroid, ...
Source: Human Cell - September 30, 2021 Category: Cytology Source Type: research

Mesenchymal stromal cells coated with anti-ACE2 antibodies might improve efficacy against COVID-19
(Source: Human Cell)
Source: Human Cell - September 30, 2021 Category: Cytology Source Type: research

Establishment of Down ’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT
AbstractDown ’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s synd rome patient by use ofSV40T-Ag andhTERT gene transfection. Expressions ofSV40T-Ag andhTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down ’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a ...
Source: Human Cell - September 29, 2021 Category: Cytology Source Type: research

Exosomes derived from mesenchymal stem cells ameliorate renal fibrosis via delivery of miR-186-5p
In this study, we found that level of miR-186-5p was significantly downregulated in TGF-β1-stimulated NRK52E cells and the obstructed kidneys of UUO mice. In addition, miR-186-5p can be transf erred from MSCs to NRK52E cells via exosomes. MSCs-delivered miR-186-5p markedly reduced the accumulation of extracellular matrix (ECM) protein, and inhibited epithelial-to-mesenchymal transition (EMT) and apoptosis in TGF-β1-stimulated NRK52E cells. Moreover, exosomal miR-186-5p from MSCs attenuat ed kidney injury and fibrosis in a UUO mouse model via inhibition of the ECM protein accumulation and EMT process. Meanwhile, d...
Source: Human Cell - September 28, 2021 Category: Cytology Source Type: research

Risk factors for recurrent positive results of the nucleic acid amplification test for COVID-19 patients: a retrospective study
This study explored whether factors other than the nucleic acid amplification test (NAAT) contribute to positive retest results. Patients with COVID-19 admitted to the Guanggu district of the Hubei Maternal and Child Health Hospital between February 17 and March 28, 2020, were retrospectively included. The patients were grouped into the negative (n = 133) and positive (n = 51) retest groups. The results showed that the proportion of patients presenting with cough was higher (P 
Source: Human Cell - September 23, 2021 Category: Cytology Source Type: research

Preparation, characterization, and antibacterial properties of hybrid nanofibrous scaffolds for cutaneous tissue engineering
AbstractSince polymeric nanofibrous scaffolds have been widely used in tissue regeneration, the risk of bacterial infections should not be neglected. In the present work, poly-caprolactone-silk fibroin-soluble eggshell membrane-silver nanoparticles (PCL-SF-SESM-AgNPs) and caprolactone-silk fibroin-soluble eggshell membrane-chitosan (PCL-SF-SESM-CS) scaffolds were fabricated via the electrospinning method for cutaneous regeneration. The composition, morphology, hydrophilicity, and mechanical features of prepared scaffolds were evaluated using Fourier transform infrared (FT-IR), scanning electron microscope (SEM), tensile, a...
Source: Human Cell - September 17, 2021 Category: Cytology Source Type: research

The protective effects of Agomelatine against A β1-42 oligomers-induced cellular senescence mediated by SIRT6 and Agomelatine’s potential in AD treatment
In conclusion, our data indicated that Agomelatine ameliorated Aβ1-42 oligomers -induced cellular senescence mediated by SIRT6, and thus, Agomelatine could be effective in treating AD. (Source: Human Cell)
Source: Human Cell - September 17, 2021 Category: Cytology Source Type: research

Establishment and characterization of NCC-LGFMS1-C1: a novel patient-derived cell line of low-grade fibromyxoid sarcoma
In conclusion, the NCC-LGFMS1-C1 cell line is a useful tool for studying LGFMS. (Source: Human Cell)
Source: Human Cell - September 17, 2021 Category: Cytology Source Type: research

Correction to: HOXA11 ‑AS promotes the migration and invasion of hepatocellular carcinoma cells by inhibiting miR‑124 expression by binding to EZH2
(Source: Human Cell)
Source: Human Cell - September 17, 2021 Category: Cytology Source Type: research

Increase of serum uric acid levels associated with APOE ε2 haplotype: a clinico-genetic investigation and in vivo approach
In conclusion, we successfully demonstrated that the ε2 haplotype, but not the ε4 haplotype, incre ases SUA levels. These findings will improve our understanding of genetic factors affecting SUA levels. (Source: Human Cell)
Source: Human Cell - September 16, 2021 Category: Cytology Source Type: research

Efficacy of IL-6 antagonists in the treatment of COVID-19 patients: further randomized controlled trials are warranted
(Source: Human Cell)
Source: Human Cell - September 15, 2021 Category: Cytology Source Type: research

Long non-coding RNA VPS9D1-AS1 promotes growth of colon adenocarcinoma by sponging miR-1301-3p and CLDN1
In conclusion, VPS9D1-AS1 facilitated cell growth and suppressed apoptosis of colon adenocarcinoma cells by sponging miR-1301-3p and upregulating CLDN1, which may be effective therapeutic strategies for patients with colon adenocarcinoma. (Source: Human Cell)
Source: Human Cell - September 14, 2021 Category: Cytology Source Type: research

Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity
AbstractEmerging evidence suggests that long non-coding RNA (lncRNA) is closely associated with numerous human diseases, including cancer. However, the functional relevance of lncRNA in human laryngeal squamous cell carcinoma (LSCC) is largely unknown. In the current study, we described CCAT2, a previously unappreciated oncogenic lncRNA in LSCC. CCAT2 was significantly upregulated in human LSCC tissue and serum samples, associated with larger tumor volume, higher clinical stage, and poorer differentiation status. Lentivirus-mediated CCAT2 knockdown notably repressed the cell viability, colony formation, and DNA synthesis r...
Source: Human Cell - September 13, 2021 Category: Cytology Source Type: research

Role of long non-coding RNAs in Down syndrome patients: a transcriptome analysis study
In conclusion, these results suggest a role of l ncRNAs in the phenotype of DS patients. (Source: Human Cell)
Source: Human Cell - September 12, 2021 Category: Cytology Source Type: research

Research progress of DLX6-AS1 in human cancers
AbstractLong non-coding RNAs (lncRNAs) are a kind of translational-repressor RNAs composed of more than 200 nucleotides and formerly considered as “transcriptional noise”. Recently studies have shown that lncRNAs could bind to multiple biomolecules such as DNA, transcription factors, RNA, chromatin complexes and proteins, and regulate target gene expression at multi-levels, thus playing an essential role in human tumors. DLX6-AS1, a recent ly discovered oncogenic lncRNA, is highly expressed in various human tumors, including lung cancer, liver cancer and pancreatic cancer. This paper mainly reviewed the regulat...
Source: Human Cell - September 11, 2021 Category: Cytology Source Type: research

Long non-coding RNA ILF3-AS1 facilitates hepatocellular carcinoma progression by stabilizing ILF3 mRNA in an m6A-dependent manner
AbstractHepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Increasing evidences have demonstrated that ILF3 antisense RNA 1 (ILF3-AS1) acts as an oncogenic long noncoding RNA (lncRNA) in several types of human cancers. However, the expression pattern, functional role and underlying mechanism of ILF3-AS1 in HCC remains largely unclear. Here, we found that ILF3-AS1 expression was significantly elevated in HCC tissues and also associated with prognosis of patients with HCC. Functional assays demonstrated that knockdown of ILF3-AS1 expression resulted in the suppression of proliferation, migration...
Source: Human Cell - September 7, 2021 Category: Cytology Source Type: research

Construction of a ceRNA network and a genomic-clinicopathologic nomogram to predict survival for HBV-related HCC
AbstractSome lncRNA-associated competing endogenous RNAs (ceRNAs) are considered as potential biomarkers for targeted therapies and prognosis in human cancer. In our present study, we aimed to construct a ceRNA network and establish a genomic-clinicopathologic nomogram to provide insights into the molecular mechanisms and predict survival for HBV-related HCC. The Cancer Genome Atlas (TCGA) database was applied to collect the data of LIHC RNA-seq dataset and miRNA-seq dataset as well as the clinicopathological information. Identification of differentially expressed RNAs (mRNAs, lncRNAs, and miRNAs) between HBV-related HCC s...
Source: Human Cell - September 6, 2021 Category: Cytology Source Type: research

Overexpression of SMC4 predicts a poor prognosis in cervical cancer and facilitates cancer cell malignancy phenotype by activating NF- κB pathway
In conclusion, these findings demonstrated that SMC4 o verexpression facilitated the development of cervical cancer cells by activating NF-κBpathway, which provides a new therapeutic target for patients with cervical cancer. (Source: Human Cell)
Source: Human Cell - September 3, 2021 Category: Cytology Source Type: research

Correction to: Mesenchymal stem cells ‑derived exosomes modulate vascular endothelial injury via miR‑144‑5p/PTEN in intracranial aneurysm
(Source: Human Cell)
Source: Human Cell - September 1, 2021 Category: Cytology Source Type: research

Establishment and characterization of NCC-ssRMS2-C1: a novel patient-derived cell line of spindle cell/sclerosing rhabdomyosarcoma
AbstractSpindle cell/sclerosing rhabdomyosarcoma (ssRMS) is a rare subtype of rhabdomyosarcoma (RMS) that has fascicular spindle cell and/or sclerosing morphology. SsRMS has a diverse molecular background and is categorized into three groups: congenital/infantile ssRMS with a gene fusion involving theNCOA2 andVGLL2, ssRMS with theMYOD1 mutation, and ssRMS with no recurrent identifiable genetic alterations. Because ssRMS is a newly defined disease concept of RMS, the optimal treatment methods have not been determined. This results in unfavorable prognosis and consequently signals the urgent need for continuous research. Pat...
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

MiR-100-5p regulates cardiac hypertrophy through activation of autophagy by targeting mTOR
AbstractAutophagy has been proved to play a vital role in cardiac hypertrophy. The present study was designed to investigate the relationship between miR-100-5p and autophagy in the development of cardiac hypertrophy. Here, miR-100-5p expression was detected in abdominal aortic coarctation (AAC)-induced cardiac hypertrophy rats and Angiotensin  II (Ang II)-stimulated cardiomyocytes. In vitro and in vivo experiments were performed to explore the function of miR-100-5p on autophagy and cardiac hypertrophy. We also investigated the mechanism of miR-100-5p on autophagy with dual-luciferase reporter assays, RNA immunopreci...
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

LncRNA ITGB2-AS1 promotes the progression of clear cell renal cell carcinoma by modulating miR-328-5p/HMGA1 axis
In this study, we aim to explore the potential function of ITGB2-AS1 in ccRCC progression and its underlying molecular mechanism. We first explored the association between ITGB2-AS1 expression level and ccRCC prognosis. We found that the expression level of ITGB2-AS1 was significantly higher in ccRCC tumor and cell lines, and highly expressed ITGB2-AS1 was also associated with a poorer prognosis. Consistently, silencing ITGB2-AS1 inhibited proliferation, promoted apoptosis in ccRCC cell lines, and curbed the tumorigenesis in the Xenograft model, reduced tumorigenesis in a xenograft tumor growth model. We further identified...
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

Profiling heterogenous sizes of circulating tumor microemboli to track therapeutic resistance and prognosis in advanced gastric cancer
This study, therefore, systematically investigated the heterogeneous si zes of CTM and their involvement in therapeutic resistance in 114 patients with advanced gastric cancer (GC) using a pre-established surface molecule-independent subtraction enrichment (SE)-iFISH strategy. CTM, which was pre-therapeutically detected in 33.3% of GC patients, can further form in anoth er 34.78% of patients following chemo-/targeted therapies. The presence of CTM is relevant to liver metastasis as well as higher CTC levels (≥ 5/6 mL). Further size-based profiling of GC-CTM revealed that CTM with 2 CTCs (CTM2) was the domina...
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

MicroRNA-1178-3p suppresses the growth of hepatocellular carcinoma by regulating transducin (beta)-like 1 X-linked receptor 1
This study aimed to investigate the role of miR-1178-3p in HCC metastasis and try to reveal the potential mechanism. In the present study, we found that miR-1178-3p was down-regulated, while TBL1XR1 was up-regulated in HCC cancer tissues by bioinformatics analysis and RT-PCR. We further confirmed the connection of miR-1178-3p and TBL1XR1 using dual-luciferase reporter (DLR) assay. Moreover, gain- and loss-of-function experiments demonstrated that overexpress miR-1178-3p inhibited cell proliferation, migration, and invasion in HCC cells and reduced the xenograft tumor growth and angiogenesis by regulating the TBL1XR1/PI3K/A...
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

Plexin domain containing protein 2 is more expressed within the invasive area of human colorectal cancer tissues
(Source: Human Cell)
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

Smoking habits and risk of COVID-19
(Source: Human Cell)
Source: Human Cell - August 9, 2021 Category: Cytology Source Type: research

Emerging roles of circUBAP2 targeting miR-370-3p in proliferation, apoptosis, and invasion of papillary thyroid cancer cells
AbstractCircular RNAs (circRNAs) have been documented to be aberrantly expressed in many types of malignancies and involved in cancer progression. However, their role in thyroid cancer (TC) remains largely unknown. Our study aimed to explore the role and mechanism of circUBAP2 in TC. The differentially expressed circRNAs in TC tissues were identified using GSE18105 from gene expression omnibus (GEO) database. CircUBAP2 and miR-370-3p expression was analyzed using qRT-PCR. The stability of circUBAP2 was confirmed by actinomycin D and RNase R. The subcellular localization of circUBAP2 was detected using cell fractionation as...
Source: Human Cell - August 3, 2021 Category: Cytology Source Type: research

FOXC2-AS1 stabilizes FOXC2 mRNA via association with NSUN2 in gastric cancer cells
AbstractLong noncoding RNA (lncRNA) FOXC2-AS1 has been reported to act as an oncogene in multiple human cancers. However, the clinical significance, functional role and underlying mechanism of FOXC2-AS1 in gastric cancer (GC) remains largely unknown. Here, we found that FOXC2-AS1 expression was significantly elevated in GC tissues and cells, and overexpression of FOXC2-AS1 indicated advanced TNM stage and shorter overall survival in GC patients. Functionally, knockdown of FOXC2-AS1 attenuated the proliferation, migration and invasion of GC cells, whereas overexpression of FOXC2-AS1 showed the opposite effects. Further inve...
Source: Human Cell - July 29, 2021 Category: Cytology Source Type: research

The Metaflammatory and Immunometabolic Role of Macrophages and Microglia in Diabetic Retinopathy
AbstractEmergent studies reveal the roles of inflammatory cells and cytokines in the development of diabetic retinopathy (DR), which is gradually portrayed as a chronic inflammatory disease accompanied by metabolic disorder. Through the pathogenesis of DR, macrophages or microglia play a critical role in the inflammation, neovascularization, and neurodegeneration of the retina. Conventionally, macrophages are generally divided into M1 and M2 phenotypes which mainly rely on glycolysis and oxidative phosphorylation, respectively. Recently, studies have found that nutrients (including glucose and lipids) and metabolites (such...
Source: Human Cell - July 29, 2021 Category: Cytology Source Type: research

Mechanical stiffness softening and cell adhesion are coordinately regulated by ERM dephosphorylation in KG-1 cells
AbstractMechanical stiffness is closely related to cell adhesion and rounding in some cells. In leukocytes, dephosphorylation of ezrin/radixin/moesin (ERM) proteins is linked to cell adhesion events. To elucidate the relationship between surface stiffness, cell adhesion, and ERM dephosphorylation in leukocytes, we examined the relationship in the myelogenous leukemia line, KG-1, by treatment with modulation drugs. KG-1 cells have ring-shaped cortical actin with microvilli as the only F-actin cytoskeleton, and the actin structure constructs the mechanical stiffness of the cells. Phorbol 12-myristate 13-acetate and staurospo...
Source: Human Cell - July 26, 2021 Category: Cytology Source Type: research

Establishment and characterization of novel patient-derived cell lines from giant cell tumor of bone
AbstractGiant cell tumor of bone (GCTB) is a locally aggressive and rarely metastasizing tumor. GCTB is characterized by the presence of unique giant cells and a recurrent mutation in the histone tail of the histone variant H3.3, which is encoded byH3F3A on chromosome 1. GCTB accounts for  ~ 5% of primary bone tumors. Although GCTB exhibits an indolent course, it has the potential to develop aggressive behaviors associated with local recurrence and distant metastasis. Currently, complete surgical resection is the only curative treatment, and novel therapeutic strategies are requi red. Patient-derived cancer c...
Source: Human Cell - July 24, 2021 Category: Cytology Source Type: research

Differential immunohistochemical expression of paraoxonase-2 in actinic keratosis and squamous cell carcinoma
(Source: Human Cell)
Source: Human Cell - July 24, 2021 Category: Cytology Source Type: research

MiR-222-3p induced by hepatitis B virus promotes the proliferation and inhibits apoptosis in hepatocellular carcinoma by upregulating THBS1
This study aimed to explore the role of miR-222-3p in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). MiR-222-3p expression in tumor tissues of HBV (+) or HBV ( −) HCC patients and corresponding cell lines was detected by quantitative reverse transcription PCR (qRT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis was evaluated by flow cytometry. The potential targets of miR-222-3p were predic ted by Targetscan, and the binding relationship between miR-222-3p and thrombospondin-1 (THBS1) was determined by luciferase reporter assay and RNA ...
Source: Human Cell - July 17, 2021 Category: Cytology Source Type: research

Mesenchymal stem cells: ideal seeds for treating diseases
AbstractMesenchymal stem cells (MSCs), a kind of multipotent stem cells with self-renewal ability and multi-differentiation ability, have become the “practical stem cells” for the treatment of diseases. MSCs have immunomodulatory properties and can be used to treat autoimmune diseases, such as systemic lupus erythematosus (SLE) and Crohn’s disease. MSCs also can be used in cancer and aging. At present, many clinical experiments are using M SCs. MSCs can reduce the occurrence of inflammation and apoptosis of tissue cells, and promote the proliferation of endogenous tissue and organ cells, so as to achieve ...
Source: Human Cell - July 16, 2021 Category: Cytology Source Type: research

Decreased prostasin expression is associated with aggressiveness of oral squamous cell carcinoma
In conclusion, we showed decreased prostasin expression was associated with aggressive features and a poorer prognosis of OSCC. (Source: Human Cell)
Source: Human Cell - July 11, 2021 Category: Cytology Source Type: research

Exosomal long non-coding RNA SOX2 overlapping transcript enhances the resistance to EGFR-TKIs in non-small cell lung cancer cell line H1975
In conclusion, our data revealed that NSCLC cells promote macrophages M2 polarization through transferring exosomal SOX2-OT, thus to  enhance its own EGFR-TKIs resistance. Mechanismly, NSCLC cells-derived exosomal SOX2-OT promotes macrophages M2 polarization via promoting Smads by sponging miR-627-3p. Our data provide a novel therapeutic target for EGFR-TKIs resistance in NSCLC. (Source: Human Cell)
Source: Human Cell - July 9, 2021 Category: Cytology Source Type: research

Mesenchymal stem cells-derived exosomes modulate vascular endothelial injury via miR-144-5p/PTEN in intracranial aneurysm
This study investigated the molecular mechanism of exosomal miR-144-5p (ex-miR-144-5p) and PTEN in IA. Ex-miR-144-5p expression was assessed in serum from individuals with ruptured intracranial aneurysm (RA) or unruptured intracranial aneurysm (UA), and healthy controls (HC). Vascular endothelial cells (VECs) were co-cultured with exosomes isolated from mesenchymal stem cells (MSCs) with transfection of miR-144-5p mimic or miR-144-5p inhibitor. IA rats were induced by combing systemic hypertension and intrathecal elastase injection. VECs were transfected with miR-144-5p mimic or inhibitor to verify the impacts of miR-144-5...
Source: Human Cell - July 8, 2021 Category: Cytology Source Type: research

Regulation of KRAS protein expression by miR-544a and KRAS-LCS6 polymorphism in wild-type KRAS sporadic colon adenocarcinoma
In this study, we investigated the KRAS status in correlation with clinicopathological features in sporadic CRC and more importantly the role oflet-7a-5p andmiR-544a-3p in the regulation of wild-type KRAS protein expression in the tumor center (T1) and invasive tumor front (T2). Analysis showed that 39.1% of tumor samples hadKRAS mutations. In wild-typeKRAS tumors, 62.0% were positive for KRAS protein expression and there was a higher percentage of KRAS-positive tumor cells and a higher intensity of immunohistochemical reaction in T2 than in T1 samples. This could not be attributed to differences inKRAS mRNA levels, sugges...
Source: Human Cell - July 7, 2021 Category: Cytology Source Type: research

Hsa_circ_0069244 acts as the sponge of miR-346 to inhibit non-small cell lung cancer progression by regulating XPC expression
We reported that circ_0069244 was remarkably down modulated in NSCLC and was linked to shorter survival and poor tumor histological grade in NSCLC patients. Functionally, circ_0069244 repressed NSC LC cell proliferation and migration. Furthermore, miR-346-5p was unveiled to be a downstream target of circ_0069244, and miR-346-5p specifically modulated XPC expression. Rescue experiments indicated that the inhibitory effect of circ_0069244 was abolished by co-expression of miR-346-5p mimics. Take n together, circ_0069244 restrained NSCLC progression by modulating the miR-346-5p/XPC axis. (Source: Human Cell)
Source: Human Cell - July 6, 2021 Category: Cytology Source Type: research

Correction to: Circular RNA circRNA_0000094 sponges microRNA ‑223‑3p and up‑regulate F‑box and WD repeat domain containing 7 to restrain T cell acute lymphoblastic leukemia progression
(Source: Human Cell)
Source: Human Cell - July 6, 2021 Category: Cytology Source Type: research