Circular RNA circ_KIAA1429 accelerates hepatocellular carcinoma progression via the miR-133a-3p/high mobility group AT-hook 2 (HMGA2) axis in an m6A-dependent manner
In this study, we evidenced that the m6A methyltransferase-like 3 (METTL3)-mediated m6A modification contributed to HCC aggressiveness through modulating a novel circ_KIAA1429/miR-133a-3p/HMGA2 axis. Specifically, circ_KIAA1429 was aberrantly overexpressed in HCC tissues and cells, and the expression levels of circ_KIAA1429 was positively regulated by METTL3 in HCC cells in a m6A-dependent manner. Then, functional experiments confirmed that deletion of both circ_KIAA1429 and METTL3 suppressed HCC cell proliferation, migration and cell mitosis in vitro and in vivo, and conversely, circ_KIAA1429 overexpression had opposite e...
Source: Human Cell - July 31, 2023 Category: Cytology Source Type: research
The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence
AbstractMesenchymal stromal cells (MSC) are promising options to cellular therapy to several clinical disorders, mainly because of its ability to immunomodulate and differentiate into different cell types. Even though MSC can be isolated from different sources, a major challenge to understanding the biological effects is that the primary cells undergo replicative senescence after a limited number of cell divisions in culture, requiring time-consuming and technically challenging approaches to get a sufficient cell number for clinical applications. Therefore, a new isolation, characterization, and expansion is necessary ever...
Source: Human Cell - July 31, 2023 Category: Cytology Source Type: research
Annexin A9 promotes cell proliferation by regulating the Wnt signaling pathway in colorectal cancer
In conclusion, ANXA9 may promote CRC progression by regulating the Wnt signaling pathway and may be a potential diagnostic biomarker in the clinical management of CRC. (Source: Human Cell)
Source: Human Cell - July 31, 2023 Category: Cytology Source Type: research
The potential role of acid ceramidase in oral squamous cell carcinoma chemo-resistance by inducing autophagy
(Source: Human Cell)
Source: Human Cell - July 29, 2023 Category: Cytology Source Type: research
RNA methylation reading protein YTHDF2 relieves myocardial ischemia –reperfusion injury by downregulating BNIP3 via m6A modification
AbstractBNIP3 is reported to be involved in hypoxia-induced mitochondrial defect and cell death in cardiomyocytes. However, little is known about the specific function and molecular mechanism of BNIP3-mediated mitophagy in myocardial ischemia –reperfusion injury (MIRI). Herein, this study explored the mechanism regulating BNIP3-modulated mitophagy in MIRI. Rat cardiomyocytes (H9c2 cells) underwent transfection and hypoxia/reoxygenation (H/R) treatment, followed by cell viability and apoptosis detection. Gain-of-function assays were con ducted in rats before MIRI modeling, followed by the monitoring of cardiac changes and...
Source: Human Cell - July 28, 2023 Category: Cytology Source Type: research
Establishment and characterization of NCC-DFSP4-C1: a novel cell line from a patient with dermatofibrosarcoma protuberans having the fibrosarcomatous transformation
In conclusion, we report a novel cell line of DFSP with fibrosarcomatous transformation, and demonstrate its utility in the development of novel therapeutic agents for DFSP. (Source: Human Cell)
Source: Human Cell - July 25, 2023 Category: Cytology Source Type: research
The miR-224-5p/SIRT3/AMPK/mTOR axis is involved in the melatonin-mediated inhibition of glucocorticoid-induced osteoporosis by activating autophagy
This study investigates the function of the miR-224-5p/SIRT3/AMPK/mTOR axis in melatonin-mediated effects against osteoporosis. Human bone marrow mesenchymal stem cells (hBMSCs) were treated with glucocorticoid dexamethasone to induce an in vitro osteoporosis model. After melatonin treatment, miR-224-5p and SIRT3 levels were measured by RT ‒PCR. Transmission electron microscopy and immunofluorescence were conducted for evaluating autophagy. Western blotting was carried out to determine the expression of osteogenesis-related proteins (Runx2, OSX, OPN, and OCN), SIRT3-AMPK-mTOR axis, and autophagy-related markers (LC3 and ...
Source: Human Cell - July 24, 2023 Category: Cytology Source Type: research
Hungatella hathewayi impairs the sensitivity of colorectal cancer cells to 5-FU through decreasing CDX2 expression
In conclusion,H. hathewayi abundance was increased in CRC tissues, and the high level ofH. hathewayi was linked to lower overall survival rate. In mechanisam,H. hathewayi treatment enhanced the 5-FU resistance of CRC cells through modulating CDX2/ β-catenin signaling. (Source: Human Cell)
Source: Human Cell - July 21, 2023 Category: Cytology Source Type: research
Chimeric antigen receptor-natural killer cells: a promising sword against insidious tumor cells
AbstractNatural killer (NK) cells are a critical component of innate immunity, particularly in initial cancer recognition and inhibition of additional tumor growth or metastasis propagation. NK cells recognize transformed cells without prior sensitization via stimulatory receptors and rapidly eradicate them. However, the protective tumor microenvironment facilitates tumor escaping via induction of an exhaustion state in immune cells, including NK cells. Hence, genetic manipulation of NK cells for specific identification of tumor-associated antigens or a more robust response against tumor cells is a promising strategy for N...
Source: Human Cell - July 21, 2023 Category: Cytology Source Type: research
Vasculature cells control neuroglial co-localization and synaptic connection in a central nervous system tissue mimic system
AbstractDespite the development of neural tissue differentiation methods using a wide variety of stem cells and compartments, there is no standardized strategy for establishing synapses. As the neuronal network is developed in parallel with blood vessel angiogenesis in the central nervous system (CNS) from the embryonic period, we examined neuron –astrocyte–vasculature interactions to understand the effect of the vasculature on the development and stabilization of neurological morphogenesis. We generated a cellular co-culture module targeting the CNS that was embedded in a collagen-based extracellular matrix (ECM) gel....
Source: Human Cell - July 20, 2023 Category: Cytology Source Type: research
Interplay between LPL and GPIHBP1 in COVID-19 patients: a possible mechanism for post-recovery cardiomyopathy
(Source: Human Cell)
Source: Human Cell - July 18, 2023 Category: Cytology Source Type: research
Musashi-1 regulates cell cycle and confers resistance to cisplatin treatment in Group 3/4 medulloblastomas cells
In this study, we investigated the clinical significance and biological functions of Musashi-1 (MSI1) in Grp3 and Grp4-MBs. First, we assessed the expression profile ofMSI1 in 59 primary MB samples (15-WNT, 18-SHH, 9-Grp3, and 17-Grp4 subgroups) by qRT-PCR.MSI1 mRNA expression levels were also validated in an additional public dataset of MBs (GSE85217). The ROC curve was used to validate the diagnostic standards ofMSI1 expression. Next, the potential correlated cell-cycle genes were measured by RNA-Seq. Cell cycle, cell viability, and apoptosis were evaluated in a Grp3/Grp4 MB cell line after knockdown ofMSI1 and cisplatin...
Source: Human Cell - July 17, 2023 Category: Cytology Source Type: research
Establishment and characterization of a novel dedifferentiated chondrosarcoma cell line, SMU-DDCS, harboring an IDH1 mutation
AbstractDedifferentiated chondrosarcoma (DDCS) is a high-grade subtype with a bi-morphic histological appearance of a conventional chondrosarcoma component and it can abruptly transition to a high-grade non-cartilaginous sarcoma. To better understand the biological features of DDCSs and to help develop new therapies, a novel DDCS cell line, SMU-DDCS, was established. Tissue from an open biopsy of a tumor resected from a 75-year-old patient was subjected to primary culture. The cell line was established and authenticated by assessing DNA microsatellite short tandem repeats. The cells maintained in monolayer cultures exhibit...
Source: Human Cell - July 15, 2023 Category: Cytology Source Type: research
KS-EMPD-1: a novel cell line of primary extramammary Paget ’s disease
AbstractExtramammary Paget ’s disease (EMPD) is a rare skin cancer that mainly occurs in apocrine sweat gland-rich areas in elderly people. The prognosis of metastatic EMPD is unfavorable because of the lack of fully effective systemic therapies. However, the difficulty in establishing a model of EMPD has hampered basic res earch for exploring its pathogenesis and optimal treatments. Here, we established for the first time an EMPD cell line (named KS-EMPD-1) from a primary tumor on the left inguinal region of an 86-year-old Japanese male. The cells were successfully maintained for more than 1 year, with a doubling tim e...
Source: Human Cell - July 11, 2023 Category: Cytology Source Type: research
Efficacy of redox nanoparticles for improving survival of transplanted cells in a mouse model of ischemic stroke
In this study, we tested the protective efficacy of these redox nanoparticles in cell culture and a mouse model of ischemic stroke. Induced human dental pulp stem cells were subjected to oxygen –glucose deprivation and reoxygenation to recapitulate ischemia and reperfusion in the penumbra surrounding a cerebral infarct. Cell viability using WST-8 assay, apoptosis using TUNEL, free radicals using MitoSOX, and inflammatory cytokines using ELISA kit were measured in the presence and absence of redox nanoparticles after oxygen–glucose deprivation and reoxygenation. The scavenging activity of redox nanoparticles against rea...
Source: Human Cell - July 7, 2023 Category: Cytology Source Type: research
