Autophagy and apoptosis: regulatory factors of chondrocyte phenotype transition in osteoarthritis
AbstractOsteoarthritis (OA) is the main pathogenic factor in diseases that cause joint deformities. As the main manifestation of the progress of OA, cartilage degradation has been closely associated with the degeneration of chondrocytes, which is induced by inflammatory factors and other trauma factors. Autophagy and apoptosis are the main mechanisms for cells to maintain homeostasis and play crucial roles in OA. Under the influence of external environmental factors (such as aging and injury), the metabolism of cells can be altered, which may affect the extent of autophagy and apoptosis. With the progression of OA, these c...
Source: Human Cell - June 5, 2023 Category: Cytology Source Type: research
Stem cell sources from human biological waste material: a role for the umbilical cord and dental pulp stem cells for regenerative medicine
AbstractStem cell research with biological waste material is an area that holds promise to revolutionize treatment modalities and clinical practice. The interest in surgical remnants is increasing with time as research on human embryonic stem cells remains controversial due to legal and ethical issues. Perhaps, these restrictions are the motivation for the use of alternative mesenchymal stem cell (MSC) sources in the regenerative field. Stem cells (SCs) of Umbilical Cord (UC) and Dental Pulp (DP) have almost similar biological characteristics to other MSCs and can differentiate into a number of cell lineages with enormous ...
Source: Human Cell - June 5, 2023 Category: Cytology Source Type: research
Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes
AbstractPeriodontitis is a chronic inflammatory disease that is the main cause of tooth loss in adults, and the key to periodontitis treatment is the repair and regenerate of periodontal bone tissue. Psoralen is the main component of thePsoralea corylifolia Linn, which shows antibacterial, anti-inflammatoryand osteogenic activities. It promotes the differentiation of periodontal ligament stem cells toward osteogenesis. Exosomes secreted by stem cells play important roles in information transmission during the osteogenic differentiation process. The aim of this paper was to investigate the role of psoralen in regulating ost...
Source: Human Cell - June 3, 2023 Category: Cytology Source Type: research
Novel insights into tumorigenesis and prognosis of endometrial cancer through systematic investigation and validation on mitophagy-related signature
In conclusion, the mitophagy-related risk score provides a novel perspective for survival and drug selection during the individual treatment of EC patients.TOMM40 serves as an oncogene in EC and promotes tumor progression via a mitophagy-related pathway. Thus,TOMM40 is a potential therapeutic target in EC. (Source: Human Cell)
Source: Human Cell - June 2, 2023 Category: Cytology Source Type: research
Downregulation of the long noncoding RNA DSCR9 (Down syndrome critical region 9) delays breast cancer progression by modulating microRNA-504-5p-dependent G protein-coupled receptor 65
AbstractPossible roles of long noncoding RNAs (lncRNAs) in cancer stem cells (CSCs) have often been reported. Here, we focused on the regulatory function of the lncRNA Down syndrome critical region 9 (DSCR9) in breast cancer stem cells (BCSCs). Through bioinformatics analysis, DSCR9, microRNA-504-5p (miR-504-5p), and G protein-coupled receptor 65 (GPR65) were identified as targets implicated in breast cancer development. Then, clinical tissue samples, breast cancer cells, and isolated BCSCs were used to determine the expression of DSCR9, miR-504-5p, and GPR65. The results confirmed the overexpression of DSCR9 and GPR65 but...
Source: Human Cell - May 29, 2023 Category: Cytology Source Type: research
From in vivo to in vitro: exploring the key molecular and cellular aspects of human female gametogenesis
AbstractHuman oogenesis is a highly complex and not yet fully understood process due to ethical and technological barriers that limit studies in the field. In this context, replicating female gametogenesis in vitro would not only provide a solution for some infertility problems, but also be an excellent study model to better understand the biological mechanisms that determine the formation of the female germline. In this review, we explore the main cellular and molecular aspects involved in human oogenesis and folliculogenesis in vivo, from the specification of primordial germ cells (PGCs) to the formation of the mature oo...
Source: Human Cell - May 26, 2023 Category: Cytology Source Type: research
Upregulation of helicase-like transcription factor predicts poor prognosis and facilitates hepatocellular carcinoma progression
In conclusion, our results suggest that upregulation of HLTF is associated with the development of HCC, and HLTF may be a potential therapeutic target for HCC treatment. (Source: Human Cell)
Source: Human Cell - May 25, 2023 Category: Cytology Source Type: research
HDAC inhibitor chidamide overcomes drug resistance in chronic myeloid leukemia with the T315i mutation through the Akt –autophagy pathway
In this study, we investigated the anti-leukemia effects of chidamide on the CML cell lines Ba/F3 P210 and Ba/F3 T315I and primary tumor cells from CML patients with the T315I mutation. The underlying mechanism was investigated, and we found that chidamide could inhibit Ba/F3 T315I cells at G0/G1 phase. Signaling pathway analysis showed that chidamide induced H3 acetylation, downregulated pAKT expression and upregulated pSTAT5 expression in Ba/F3 T315I cells. Additionally, we found that the antitumor effect of chidamide could be exerted by regulating the crosstalk between apoptosis and autophagy. When chidamide was used in...
Source: Human Cell - May 24, 2023 Category: Cytology Source Type: research
Roles of non-coding RNAs in the metabolism and pathogenesis of bladder cancer
AbstractBladder cancer (BC) is featured as the second most common malignancy of the urinary tract worldwide with few treatments leading to high incidence and mortality. It stayed a virtually intractable disease, and efforts to identify innovative and effective therapies are urgently needed. At present, more and more evidence shows the importance of non-coding RNA (ncRNA) for disease-related study, diagnosis, and treatment of diverse types of malignancies. Recent evidence suggests that dysregulated functions of ncRNAs are closely associated with the pathogenesis of numerous cancers including BC. The detailed mechanisms unde...
Source: Human Cell - May 20, 2023 Category: Cytology Source Type: research
EphA1 aggravates neuropathic pain by activating CXCR4/RhoA/ROCK2 pathway in mice
In conclusion, our study suggests that depletion of EphA1 ameliorates neuropathic pain by modulating the CXCR4/RhoA/ROCK2 signaling pathway, and targeting EphA1 may be a potential clinical treatment for neuropathic pain. (Source: Human Cell)
Source: Human Cell - May 10, 2023 Category: Cytology Source Type: research
ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models
AbstractE3 ubiquitin ligase Zinc and Ring Finger 2 (ZNRF2) has been demonstrated to be engaged in the development of multiple cancers. Nevertheless, the function of ZNRF2 in breast cancer (BC) still unclear. In this work, we firstly analyzed the differentially expressed genes in BC by bioinformatics and found that ZNRF2 was highly expressed in BC. Consistently, we further confirmed that ZNRF2 was upregulated in BC tissues compared with adjacent normal tissues, and this was positively correlated with the poor prognosis and the higher pathological grades of patients with BC. Functional assays performed on HCC1937 and MCF-7 c...
Source: Human Cell - May 10, 2023 Category: Cytology Source Type: research
Role of cuproptosis in understanding diseases
AbstractCell death is involved in a wide range of physiological and pathological processes. Recently, the term “cuproptosis” was coined to describe a novel type of cell death. This type of cell death, characterized by copper accumulation and proteotoxic stress, is a copper-dependent manner of death. Despite the progress achieved toward a better understanding of cuproptosis, mechanisms and related signali ng pathways in physiology and pathology across various diseases remain to be proved. This mini review summarizes current research on cuproptosis and diseases, providing insights into prospective clinical therapies via ...
Source: Human Cell - May 8, 2023 Category: Cytology Source Type: research
Multi-omics data integration reveals the molecular network of dysregulation IQGAP2-mTOR promotes cell proliferation
AbstractIQGAP2 as a tumor suppressor gene can influence cell proliferation in multiple tumor cell lines. However, the regulation network of cell proliferation resulting solely from the deficiency ofIQGAP2 in cells was still unclear. Here, we integrated transcriptome, proteome, and phosphoproteome analyses to investigate the regulatory network of cell proliferation inIQGAP2 knockdown HaCaT and HEK293 cells. Our findings revealed that the dysregulation of the IQGAP2-mTOR molecular network led to increased cell proliferation. We demonstrated thatIQGAP2 knockdown enhanced the phosphorylation levels of AKT and S6K, leading to i...
Source: Human Cell - May 8, 2023 Category: Cytology Source Type: research
TEAD4 predicts poor prognosis and transcriptionally targets PLAGL2 in serous ovarian cancer
AbstractThe oncogenic function of TEA domain transcription factor 4 (TEAD4) has been confirmed in multiple human malignancies, while its potential role and regulatory mechanism in serous ovarian cancer progression are left unknown. By the gene expression analyses from Gene Expression Profiling Interactive Analysis (GEPIA) database, TEAD4 expression is shown to be up-regulated in serous ovarian cancer samples. Here, we confirmed the high expression of TEAD4 in clinical serous ovarian cancer specimens. In the following functional experiments, we found that TEAD4 overexpression promoted serous ovarian cancer malignant phenoty...
Source: Human Cell - May 5, 2023 Category: Cytology Source Type: research
Correction: PTEN-induced kinase 1 enhances the reparative effects of bone marrow mesenchymal stromal cells on mice with renal ischaemia/reperfusion-induced acute kidney injury
(Source: Human Cell)
Source: Human Cell - May 5, 2023 Category: Cytology Source Type: research
