TRIM59 promotes steatosis and ferroptosis in non-alcoholic fatty liver disease via enhancing GPX4 ubiquitination
AbstractNon-alcoholic fatty liver disease (NAFLD) is the most common liver disease around the world. However, no specific medicine has been approved for NAFLD treatment. Our study was conducted to explore the role and mechanism of TRIM59 in NAFLD, aiming to provide a novel target for NAFLD treatment. Here, the expression of TRIM family members was detected in 10 mild and severe NAFLD tissues as well as 10 normal tissues. TRIM59 expression was verified in 10 normal tissues and 25 mild and severe NAFLD tissues. Palmitic acid and high-fatty diet were used for the construction of NAFLD models. Oil Red O staining was used to de...
Source: Human Cell - November 22, 2022 Category: Cytology Source Type: research
Identification and validation of an m6A-related gene signature to predict prognosis and evaluate immune features of breast cancer
In conclusion, the m6A-related gene signature may be a sensitive biomarker for overall survi val prediction and guide the individualized treatment for breast cancer patients. (Source: Human Cell)
Source: Human Cell - November 20, 2022 Category: Cytology Source Type: research
METTL14 upregulates TCF1 through m6A mRNA methylation to stimulate osteogenic activity in osteoporosis
AbstractAlteration of N6-methyladenosine (m6A) is closely linked to spanning biological processes including osteoporosis (OP) development. This research focuses on the function of methyltransferase like 14 (METTL14) in bone turnover and its interaction with T cell factor 1 (TCF1). A mouse model of OP was established by ovariectomy (OVX). The bone mass parameters were evaluated by micro-CT analysis. Mouse MC3T3-E1 cells and mouse bone marrow macrophages (BMMs) were induced for osteogenic or osteoclastic differentiation, respectively, for in vitro experiments. The osteogenesis or osteoclasis activity was analyzed by measurin...
Source: Human Cell - November 19, 2022 Category: Cytology Source Type: research
MicroRNA-217 aggravates breast cancer through activation of NF1-mediated HSF1/ATG7 axis and c-Jun/ATF3/MMP13 axis
AbstractApplication of microRNA-mediated mRNA expression in treatment of diverse cancers has been documented. The current study was explored to study the role of miR-217 in breast cancer (BC) progression and the related downstream factors. Clinical tissue samples, BC cell lines and the established xenograft models were prepared for ectopic expression and depletion experiments to discern the regulatory roles of miR-217-mediated NF1 in BC cell proliferation, metastasis and chemoresistance as well as tumorigenic ability of BC cells in nude mice. miR-217 was upregulated in BC, which was a predictor of poor prognosis of BC pati...
Source: Human Cell - November 10, 2022 Category: Cytology Source Type: research
Cl-amidine attenuates lipopolysaccharide-induced inflammation in human gingival fibroblasts via the JNK/MAPK, NF- κB, and Nrf2 signalling pathways
AbstractCl-amidine has been reported to have anti-inflammatory properties in a variety of diseases. However, the role of Cl-amidine in periodontal disease remains unclear. Here, the purpose of this study was to investigate the effect of Cl-amidine on lipopolysaccharide (LPS)-induced inflammation in human gingival fibroblasts (HGFs). The cytotoxic effect of Cl-amidine was measured with the Cell Counting Kit-8 (CCK-8) assay and Annexin V-FITC/PI staining. The protein levels of IL-6 and IL-8 in culture supernatants were measured with enzyme-linked immunosorbent assay (ELISA). The mRNA levels of inflammatory cytokines,TLR4 and...
Source: Human Cell - November 9, 2022 Category: Cytology Source Type: research
The exploitation of enzyme-based cancer immunotherapy
AbstractCancer immunotherapy utilizes the immune system and its wide-ranging components to deliver anti-tumor responses. In immune escape mechanisms, tumor microenvironment-associated soluble factors and cell surface-bound molecules are mainly accountable for the dysfunctional activity of tumor-specific CD8+ T cells, natural killer (NK) cells, tumor associated macrophages (TAMs) and stromal cells. The myeloid-derived suppressor cells (MDSCs) and Foxp3+ regulatoryT cells (Tregs), are also key tumor-promoting immune cells. These potent immunosuppressive networks avert tumor rejection at various stages, affecting immunothe...
Source: Human Cell - November 5, 2022 Category: Cytology Source Type: research
PRMT5 promotes retinoblastoma development
In conclusion, this study clarifies that the depletion of PRMT5 reduces H3K4me3-mediated VEGFA transcription and retards the carcinogenesis of RB by suppressing the expression of MMPs. (Source: Human Cell)
Source: Human Cell - November 4, 2022 Category: Cytology Source Type: research
Panobinostat inhibits breast cancer progression via Vps34-mediated exosomal pathway
This study was set out from recent evidence that exosome was a mechanism of intercellular drug transfer with significant pharmacological consequence s. It enlightened us LBH589 might regulate tumor growth through exosomal secretion. Here we demonstrated LBH589 induced autophagy and facilitated secretory autophagy. Furthermore, LBH589 dose- and time-dependently stimulated exosomal release mediated by Vps34/Rab5C pathway, documented by the ablatio n of Vps34 and/or Rab5C in breast cancer cells. Additionally, the findings also presented LBH589 inhibited breast cancer progression via exosomes. Altogether, we revealed a novel m...
Source: Human Cell - November 3, 2022 Category: Cytology Source Type: research
Exosomal circ_0091741 promotes gastric cancer cell autophagy and chemoresistance via the miR-330-3p/TRIM14/Dvl2/Wnt/ β-catenin axis
In conclusion, our study emphas ized the promotive role of exosomal circ_0091741 in autophagy and chemoresistance of GC cells, thus laying the basis for the development of novel therapeutic targets for GC treatment. (Source: Human Cell)
Source: Human Cell - November 3, 2022 Category: Cytology Source Type: research
Establishment, characterization and functional testing of two novel ex vivo extraskeletal myxoid chondrosarcoma (EMC) cell models
In conclusion, two novel EMC ex vivo cell models (USZ20-EMC1 and USZ22-EMC2) were successfully established and characterized from native tumor tissues. Both cell models will be useful tools for further investigating disease mechanisms and for answering basic and translational research questions. (Source: Human Cell)
Source: Human Cell - November 1, 2022 Category: Cytology Source Type: research
Dental follicle cells show potential for treating Parkinson ’s disease through dopaminergic-neuronogenic differentiation
This study indicated that hDFCs might be a promising source of dopaminergic neurons for functional transplantation, and encouraged further detailed studies on the potential of hDFCs for treating PD. (Source: Human Cell)
Source: Human Cell - September 28, 2022 Category: Cytology Source Type: research
Odd-skipped related 1 plays a tumor suppressor role in ovarian cancer via promoting follistatin-like protein 1 transcription
In conclusion, these results indicated that OSR1 may function as a tumor suppressor through augmenting FSTL1 transcription in OC progression, suggesting that the OSR1/ FSTL1 axis may exhibit potential as a therapeutic target for OC therapy. (Source: Human Cell)
Source: Human Cell - September 28, 2022 Category: Cytology Source Type: research
Exploring prognostic value and regulation network of PPP1R1A in hepatocellular carcinoma
This study may help to clarify the function and mechanism of PPP1R1A in HCC and provide a potential biomarker for tumor prevention and treatment. (Source: Human Cell)
Source: Human Cell - September 28, 2022 Category: Cytology Source Type: research
Long non-coding RNA RPL34-AS1 ameliorates oxygen –glucose deprivation-induced neuronal injury via modulating miR-223–3p/IGF1R axis
In this study, RPL34-AS1 expression was reduced in patients suffering from ischemic s troke. The overexpression of RPL34-AS1 reduced ischemic brain damage. However, the cell viability and glucose uptake were increased, and the apoptosis rate was decreased in the OGD/R-induced neurons. Further, miR-223-3p resulted in the decreased cell viability and glucose uptake and the increased ce ll apoptosis to cause ischemic brain damage. Besides, the neuroprotective effects of RPL34-AS1 on OGD/R injury were partly reversed by miR-223–3p. Mechanistically, lncRNA RPL34-AS1 could function as the competing endogenous RNA (ceRNA) of mi...
Source: Human Cell - September 28, 2022 Category: Cytology Source Type: research
Pirfenidone promotes the levels of exosomal miR-200 to down-regulate ZEB1 and represses the epithelial-mesenchymal transition of non-small cell lung cancer cells
In conclusion, PFD-induced miR-200 overexpression in CAFs inhibits ZEB1 expression in NSCLC cells, and thus decelerates the migration, invasion and EMT process. Our study may provide clues for the treatment of NSCLC. (Source: Human Cell)
Source: Human Cell - September 28, 2022 Category: Cytology Source Type: research
