Accelerated T-cell exhaustion: its pathogenesis and potentially severe outcomes
(Source: Human Cell)
Source: Human Cell - January 1, 2023 Category: Cytology Source Type: research
SARS-CoV-2 and Toxoplasma gondii shared symptoms suggest muscle cells and neurons are their chronic infection reservoirs
(Source: Human Cell)
Source: Human Cell - January 1, 2023 Category: Cytology Source Type: research
Correction to: Establishment and characterization of NCC-GCTB5-C1: a novel cell line of giant cell tumor of bone
(Source: Human Cell)
Source: Human Cell - January 1, 2023 Category: Cytology Source Type: research
Correction to: Establishment and characterization of NCC-MRT1-C1: a novel cell line of malignant rhabdoid tumor
(Source: Human Cell)
Source: Human Cell - January 1, 2023 Category: Cytology Source Type: research
Expansion of human amniotic epithelial cells using condition cell reprogramming technology
In this study, we used an optimized CRC protocol to successfully culture hAECs in a CRC medium supplemented with xenogen-free human serum. We established that hAECs thrive under the CRC conditions for over 5 passages while still expressing pluripotent stem markers (OCT-4, SOX-2 and NANOG) and non-immunogenic markers (CD80, CD86 and HLA-G) suggesting that even late-passage hAECs retain their privileged phenotype. The hAECs-CRC cells were infected with a puromycin-selectable lentivirus expressingluciferase andGFP (green fluorescent protein) and stably selected with puromycin. The hAECs expressing GFP were injected subcutaneo...
Source: Human Cell - December 31, 2022 Category: Cytology Source Type: research
Piezo protein determines stem cell fate by transmitting mechanical signals
AbstractPiezo ion channel is a mechanosensitive protein on the cell membrane, which contains Piezo1 and Piezo2. Piezo channels are activated by mechanical forces, including stretch, matrix stiffness, static pressure, and shear stress. Piezo channels transmit mechanical signals that cause different downstream responses in the differentiation process, including integrin signaling pathway, ERK1/2 MAPK signaling pathway, Notch signaling, and WNT signaling pathway. In the fate of stem cell differentiation, scientists found differences in Piezo channel expression and found that Piezo channel expression is related to developmenta...
Source: Human Cell - December 29, 2022 Category: Cytology Source Type: research
Silencing RPL8 inhibits the progression of hepatocellular carcinoma by down-regulating the mTORC1 signalling pathway
In conclusion,RPL8 may affect LIHC progression by regulating the mTORC1 signalling pathway. (Source: Human Cell)
Source: Human Cell - December 28, 2022 Category: Cytology Source Type: research
Expression of estrogen receptors, PELP1, and SRC in human spermatozoa and their associations with semen quality
In this study, we obtained 119 samples from male participants of Caucasian descent who donated semen for standard analysis. We analyzed gene expression of estrogen receptors (ESR1 andESR2) and their coregulators —proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), and cellular kinase c-Src (SRC). RNA level was established using reverse-transcribed RNA as a template, followed by a polymerase chain reaction. Proteins ’ presence was confirmed by western blot and immunocytochemistry techniques. “Normal” values of semen parameters were defined as follows: > 32% sperm with progressive motility, > ...
Source: Human Cell - December 28, 2022 Category: Cytology Source Type: research
The roles of ETS transcription factors in liver fibrosis
This article reviews the research progress of ETS family members in the development liver fibrosis as well as their prospect of clinical application. (Source: Human Cell)
Source: Human Cell - December 22, 2022 Category: Cytology Source Type: research
Heterophyllin B inhibits the malignant phenotypes of gastric cancer cells via CXCR4
This study intended to further explore the effects and modulatory mechanism of HB in gastric cancer (GC) cells. The binding relationship between HB and CXCR4 was investigated by network pharmacological analysis, molecular docking, and cellular thermal shift assay (CETSA)-WB assay. Cellular assays revealed that HB could restrain GC cell viability, proliferation, invasion and migration by binding to CXCR4. Further studies presented that HB could suppress PI3K/AKT signaling pathway via binding to CXCR4, thus repressing PD-L1 expression. In vivo experiments in nude mice demonstrated that HB constrained PI3K/AKT signaling pathw...
Source: Human Cell - December 21, 2022 Category: Cytology Source Type: research
Recent topics and advanced therapies in chronic granulomatous disease
AbstractChronic granulomatous disease (CGD) is a primary immunodeficiency characterized by the inability of phagocytes to produce reactive oxygen species (ROS) owing to a defect in any of the five components (CYBB/gp91phox,CYBA/p22phox,NCF1/p47phox,NCF2/p67phox, andNCF4/p40phox) and a concomitant regulatory component ofRac1/2 andCYBC1/Eros of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. Patients with CGD are at an increased risk of life-threatening infections caused by catalase-positive bacteria and fungi and of inflammatory complications such as CGD colitis. Antimicrobial and azole antifungal p...
Source: Human Cell - December 19, 2022 Category: Cytology Source Type: research
Roles of RNA-binding proteins in neurological disorders, COVID-19, and cancer
AbstractRNA-binding proteins (RBPs) have emerged as important players in multiple biological processes including transcription regulation, splicing, R-loop homeostasis, DNA rearrangement, miRNA function, biogenesis, and ribosome biogenesis. A large number of RBPs had already been identified by different approaches in various organisms and exhibited regulatory functions on RNAs ’ fate. RBPs can either directly or indirectly interact with their target RNAs or mRNAs to assume a key biological function whose outcome may trigger disease or normal biological events. They also exert distinct functions related to their canonical...
Source: Human Cell - December 18, 2022 Category: Cytology Source Type: research
Microfibril-associated protein 2 is activated by POU class 2 homeobox 1 and promotes tumor growth and metastasis in tongue squamous cell carcinoma
AbstractTongue squamous cell carcinoma (TSCC) represents the most frequent malignancy of the oral cavity, characterized by a high metastasis rate and poor prognosis. Microfibril-associated protein 2 (MFAP2), as an extracellular matrix protein, has been found to drive tumor progression. The function and underlying mechanism of MFAP2 in TSCC remain unknown. The expression levels of MFAP2 were analyzed in tissue samples from 30 TSCC patients by real time-polymerase chain reaction and western blot assays. Our results revealed that the expression of MFAP2 mRNA and protein was upregulated in TSCC tissue samples compared with tha...
Source: Human Cell - December 17, 2022 Category: Cytology Source Type: research
Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2
AbstractAlthough programmed cell death 4 (PDCD4) was initially reported as a tumor suppressor and has been shown to inhibit cancer cell growth and metastasis, recent studies have demonstrated that loss of PDCD4 expression also induces growth inhibition by inducing apoptosis and/or cellular senescence. At present, the roles of PDCD4 in the activation and profibrogenic properties of myofibroblasts, which are critically involved in organ fibrosis, such as that in the liver, are unclear. We, therefore, investigated the roles of PDCD4 in myofibroblasts using human hepatic stellate cell line Lieming Xu-2 (LX-2). PDCD4 knockdown ...
Source: Human Cell - December 16, 2022 Category: Cytology Source Type: research
Construction of a m5C-related long non-coding RNA signature for the prognosis of hepatocellular carcinoma
AbstractRNA modification serves as a kind of posttranscriptional modification. Besides N6-methyladenosine (m6A), 5-methylcytosine(m5C) is also an important RNA modification. Long non-coding RNAs (lncRNAs) play an important role in tumor progression. Thus, we performed bioinformatic analysis to establish a m5C-related lncRNA signature(m5ClncSig) for hepatocellular carcinoma (HCC). The RNA sequencing data and clinical data were obtained from The Cancer Genome Atlas (TCGA) database. Pearson correlation coefficient analysis was applied to conduct m5C-related genes and m5C-related lncRNAs co-expressing network. Univariate Cox r...
Source: Human Cell - December 15, 2022 Category: Cytology Source Type: research
