Establishment and comprehensive analysis of a new human cell line (NK-NJ) with NK-cell characteristics established from extranodal natural killer cell lymphoma/leukemia
In conclusion, we established a new ENKTL cell line in the era of new targeted drugs. We hope that this cell line can help to further understand underlying pathogenesis of ENKTL especially for advanced ENKTL and the functional role of EBV in ENKTL pathogenetic process. (Source: Human Cell)
Source: Human Cell - December 15, 2022 Category: Cytology Source Type: research
Identification and validation of a novel long non-coding RNA (LINC01465) in ovarian cancer
AbstractEpithelial Ovarian Cancer (EOC) is a heterogeneous disease usually diagnosed at advanced stages. Therefore, early detection is crucial for better survival. Despite the advances in ovarian research, mechanisms underlying EOC carcinogenesis are not elucidated. We performed chromatin immunoprecipitation sequencing to identify genes regulated by E2F5, a transcription factor involved in ovarian carcinogenesis. Results revealed several putative candidate genes (115 protein-coding genes, 20 lncRNAs, 6 pseudogenes, and 4 miRNAs). A literature review and bioinformatics analysis of these genes revealed a novel lncRNA candida...
Source: Human Cell - December 13, 2022 Category: Cytology Source Type: research
Knockdown of lncRNA-ASLNC12002 alleviates epithelial –mesenchymal transition of type II alveolar epithelial cells in sepsis-induced acute respiratory distress syndrome
AbstractPatients with sepsis-induced acute respiratory distress syndrome (ARDS) have higher mortality and poor prognosis than pneumonia-induced ARDS. Pulmonary fibrosis is an irreversible accumulation of connective tissue in the interstitium of the lung and closely associated with the epithelial –mesenchymal transition (EMT) of type II alveolar epithelial cells (AECIIs). Therefore, it is undoubtedly worth studying whether the EMT of AECIIs in sepsis-induced ARDS patients is different from that in patients with pneumonia-induced ARDS in the regulatory mechanism. Here, we will report for th e first time that an lncRNA-ASLN...
Source: Human Cell - December 7, 2022 Category: Cytology Source Type: research
Ex vivo chemosensitivity assay using primary ovarian cancer organoids for predicting clinical response and screening effective drugs
AbstractSelecting the best treatment for individual patients with cancer has attracted attention for improving clinical outcomes. Recent progress in organoid culture may lead to the development of personalized medicine. Unlike molecular-targeting drugs, there are no predictive methods for patient response to standard chemotherapies for ovarian cancer. We prepared organoids using the cancer tissue-originated spheroid (CTOS) method from 61 patients with ovarian cancer with 100% success rate. Chemosensitivity assays for paclitaxel and carboplatin were performed with 84% success rate using the primary organoids from 50 patient...
Source: Human Cell - December 7, 2022 Category: Cytology Source Type: research
Accelerated T-cell exhaustion: its potential role in autoimmune disease and hyperinflammatory disease pathogenesis
(Source: Human Cell)
Source: Human Cell - December 7, 2022 Category: Cytology Source Type: research
Retraction Note: LINC00665/miR-9-5p/ATF1 is a novel axis involved in the progression of colorectal cancer
(Source: Human Cell)
Source: Human Cell - December 6, 2022 Category: Cytology Source Type: research
The TRIM family as a potential target for nonalcoholic fatty liver disease
(Source: Human Cell)
Source: Human Cell - December 5, 2022 Category: Cytology Source Type: research
Heterogeneous expression and role of receptor tyrosine kinase-like orphan receptor 2 (ROR2) in small cell lung cancer
AbstractThe present study investigated the expression and role of ROR2 in small cell lung cancer (SCLC). To examine the expression of ROR2, 27 surgically resected SCLC tissue samples were immunostained for ROR2. Sixteen tissue samples were positive and some showed intratumor heterogeneity in staining intensity. The heterogeneity of ROR2 expression was also observed in tumor tissues from a PDX model of SCLC, in which there were cells with high ROR2 expression (ROR2high cells) and without its expression (ROR2low cells). These cells were subjected to a RNA sequence analysis. GSEA was performed and the results obtained reveale...
Source: Human Cell - December 4, 2022 Category: Cytology Source Type: research
Novel CSF1R-positive tenosynovial giant cell tumor cell lines and their pexidartinib (PLX3397) and sotuletinib (BLZ945)-induced apoptosis
AbstractTenosynovial giant cell tumor (TGCT) is a mesenchymal tumor derived from the synovium of the tendon sheath and joints, most frequently in the large joints. The standard of care for TGCTs is surgical resection. A new targeting approach for treating TGCTs has emerged from studies on the role of the CSF1/CSF1 receptor (CSF1R) in controlling cell survival and proliferation during the pathogenesis of TGCTs. We established four novel cell lines isolated from the primary tumor tissues of patients with TGCTs. The cell lines were designated Si-TGCT-1, Si-TGCT-2, Si-TGCT-3, and Si-TGCT-4, and the TGCT cells were characterize...
Source: Human Cell - December 2, 2022 Category: Cytology Source Type: research
DNA repair/recombination protein 54L promotes the progression of lung adenocarcinoma by activating mTORC1 pathway
In conclusion, the E2F7-RAD54L axis promotes the progression of LUAD through the mTORC1 signalling pathway. (Source: Human Cell)
Source: Human Cell - December 1, 2022 Category: Cytology Source Type: research
Mitochondria: how eminent in ageing and neurodegenerative disorders?
AbstractNumerous factors are implicated in the onset and progression of ageing and neurodegenerative disorders, with defects in cell energy supply and free radicals regulation designated as being the main functions of mitochondria and highly accentuated in plentiful studies. Hence, analysing the role of mitochondria as one of the main factors implicated in these disorders could undoubtedly come in handy with respect to disease prevention and treatment. In this review, first, we will explore how mitochondria account for neurodegenerative disorders and ageing and later will draw the various pathways contributing to mitochond...
Source: Human Cell - November 29, 2022 Category: Cytology Source Type: research
Single-cell and microarray chip analysis revealed the underlying pathogenesis of ulcerative colitis and validated model genes in diagnosis and drug response
In this study, we sought to develop robust diagnostic biomarkers for UC, to uncover potential targets for anti-TNF- ɑ drugs, and to investigate their associated pathway mechanisms. We collected single-cell expression profile data from 9 UC or healthy samples and performed cell annotation and cell communication analysis. Revealing the possible pathogenesis of ulcerative colitis by Gene Ontology (GO), Kyoto Encycl opedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) analysis. Based on the disease-related modules obtained from weighted correlation network analysis (WGCNA) analysis, we used Lasso regres...
Source: Human Cell - November 29, 2022 Category: Cytology Source Type: research
Repeated controlled ovarian stimulation-induced ovarian and uterine damage in mice through the PI3K/AKT signaling pathway
In conclusion, repeated COS causes ovarian and uterus damage possibly through the PI3K/AKT signaling pathway, and this finding may provide some experimental basis for guiding clinical treatment. (Source: Human Cell)
Source: Human Cell - November 28, 2022 Category: Cytology Source Type: research
Establishment and characterization of NCC-PLPS2-C1: a novel cell line of pleomorphic liposarcoma
In conclusion, we report the first PLPS cell line from a tumor specimen that was morphologically accompanied by pleomorphic and born-forming foci. We believe that NCC-PLPS2-C1 will be useful for the development of novel chemotherapies for PLPS. (Source: Human Cell)
Source: Human Cell - November 27, 2022 Category: Cytology Source Type: research
Extracellular vesicles derived from cancer-associated fibroblasts carry tumor-promotive microRNA-1228-3p to enhance the resistance of hepatocellular carcinoma cells to sorafenib
AbstractCancer-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) can promote tumor progression by delivering microRNA (miRNA). Whether EVs could transfer miR-1228-3p into hepatocellular carcinoma (HCC) cells to affect chemoresistance was discussed in this study. Normal fibroblasts (NFs) and CAFs were isolated from tissue samples of HCC patients. We assessed the functions of HCC cells after co-culturing with NFs and CAFs. miR-1228-3p gain-of-function experiments were conducted. Next, functional assays were carried out to determine the binding of miR-1228-3p to placenta associated 8 (PLAC8). In vivo models w...
Source: Human Cell - November 23, 2022 Category: Cytology Source Type: research
