The interferon-stimulated genes are plausibly a primary genetic locus for genetically enabling hyperinflammatory and autoimmune diseases and lymphocyte exhaustion
(Source: Human Cell)
Source: Human Cell - October 19, 2023 Category: Cytology Source Type: research
tRNAThr-miR-720 mimicry in glioma cells
(Source: Human Cell)
Source: Human Cell - October 19, 2023 Category: Cytology Source Type: research
Therapeutic potential of miRNAs in placental extracellular vesicles in ovarian and endometrial cancer
In this study, by RNA sequencing of placental EVs, 20 highly expressed microRNAs (miRNAs) in placental EVs were selected and analysed for their functions on ovarian and endometrial cancer. There were up to seven enriched miRNAs, including miRNA-199a-3p, miRNA-143-3p, and miRNA-519a-5p in placental EVs showing effects on the inhibition of ovarian and endometrial cancer cell proliferation and migration, and promotion of cancer cell death, reported in the literature. Most of these miRNAs have been reported to be downregulated in ovarian and endometrial cancer. Transfection of ovarian and endometrial cancer cells with mimics o...
Source: Human Cell - October 6, 2023 Category: Cytology Source Type: research
Activation of cell adhesion and migration is an early event of platelet-rich plasma (PRP)-dependent stimulation of human adipose-derived stem/stromal cells
AbstractStem cell therapy is a promising treatment in regenerative medicine. Human adipose-derived stem/stromal cells (hASCs), a type of mesenchymal stem cell, are easy to harvest. In plastic and aesthetic surgery, hASC may be applied in the treatment of fat grafting, wound healing, and scar remodeling. Platelet-rich plasma (PRP) contains various growth factors, including platelet-derived growth factor (PDGF), which accelerates wound healing. We previously reported that PRP promotes the proliferation of hASC via multiple signaling pathways, and we evaluated the effect of PRP on the stimulation of hASC adhesion and migratio...
Source: Human Cell - October 3, 2023 Category: Cytology Source Type: research
MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer
AbstractOvarian cancer is the common cause of cancer-related death in women and is considered the most deadly gynecological cancer. It has been established that GATA-binding protein 6 (GATA6) is abnormally expressed in several types of malignant tumors and acts as an oncogenic protein or a tumor suppressor. However, the underlying mechanism of GATA6 in ovarian cancer progression has not been elucidated. Data in the present study revealed that GATA6 expression was negatively correlated to microRNA-10a-5p (miR-10a-5p) in ovarian cancer tissue and cells and that GATA6 is directly targeted by miR-10a-5p. Notably, upregulated m...
Source: Human Cell - September 28, 2023 Category: Cytology Source Type: research
Isolation of tumor stem-like cells from primary laryngeal squamous cell carcinoma cells (FD-LS-6)
In this study, we establish an LSCC cell line (FD-LS-6) from primary LSCC tumor tissue (not experienced single-cell cloning) and adapted a culturing condition for the expansion of potential stem cells (EPSCs) to isolate CSCs from FD-LS-6. We successfully derived novel CSCs and named them as LSCC sphere-forming cells (LSCSCs) which were subsequently characterized for their CSC properties. We showed that LSCSCs shared many properties of CSCs, including CSC marker, robust self-renewal capacity, tumorigenesis ability, potential to generate other cell types such as adipocytes and osteoblasts, and resistance to chemotherapy. Com...
Source: Human Cell - September 27, 2023 Category: Cytology Source Type: research
Copper in Cancer: from transition metal to potential target
AbstractIn recent years, with the continuous in-depth exploration of the molecular mechanisms of tumorigenesis, numerous potential new targets for cancer treatment have been identified, some of which have been further developed in clinical practice and have produced positive outcomes. Notably, researchers' initial motivation for studying copper metabolism in cancer stems from the fact that copper is a necessary trace element for organisms and is closely connected to body growth and metabolism. Moreover, over the past few decades, considerable progress has been made in understanding the molecular processes and correlations ...
Source: Human Cell - September 26, 2023 Category: Cytology Source Type: research
The effect of SARS-CoV-2 on the development of Parkinson's disease: the role of α-synuclein
AbstractThe current coronavirus disease 2019 (COVID-19) can lead to various neurological complications in infected people. These neurological effects include problems in both central nervous system (CNS) and peripheral nervous system (PNS). Hyposmia, a PNS symptom of COVID-19, frequently manifests in the early stages of Parkinson's disease (PD) and serves as an early warning sign of the condition. In addition, the olfactory system is recognized as an early site for the onset of α-synuclein pathology, the pathological hallmark of PD. PD is characterized by accumulation and aggregation of misfolded α-synuclein (α-Syn) int...
Source: Human Cell - September 21, 2023 Category: Cytology Source Type: research
TRIM28 recruits E2F1 to regulate CBX8-mediated cell proliferation and tumor metastasis of ovarian cancer
In this study, we found that CBX8 was upregulated in OC tissues originating from GEPIA and TNM databases, OC patients ’ samples from hospital, and OC cell lines. Furthermore, CBX8 knockdown by short hairpin RNA (shRNA) technology markedly inhibited proliferation and invasion, induced migration, cell cycle arrest, and apoptosis in vitro. Mechanistically, CBX8 activated PI3K/AKT/mTOR signaling pathway to take effec t. In addition, TRIM28 and E2F1 were enriched in OC tissues from the TNM database and OC patients’ samples similar to the results of CBX8. Correlation analysis indicated positive correlations among TRIM28, E2F...
Source: Human Cell - September 14, 2023 Category: Cytology Source Type: research
Establishment and characterization of BMC-PDC-019: a novel patient-derived cell line of EGFR-mutant pulmonary adenocarcinoma transformed into small-cell lung cancer
We examined a range of proliferation-inhibiting effects of anti-cancer drugs currently used for de novo SCLC, using BMC-PDC-019 cells. We concluded that BMC-PDC-019 would be a useful tool for pre-clinical and translational research. (Source: Human Cell)
Source: Human Cell - September 14, 2023 Category: Cytology Source Type: research
A short-term three dimensional culture-based drug sensitivity test is feasible for malignant bone tumors
AbstractThe feasibility of a short-term, three-dimensional (3D) culture –based drug sensitivity test (DST) for surgically resected malignant bone tumors, including osteosarcoma (OS), was evaluated utilizing two OS cell line (KCS8 or KCS9)–derived xenograft (CDX) models. Twenty-three (KCS8) or 39 (KCS9) of 60 tested drugs were likely effective in OS cells derived fro m a cell line before xenografting. Fewer drugs (19: KCS8, 26: KCS9) were selected as effective drugs in cells derived from a CDX tumor, although the drug sensitivities of 60 drugs significantly correlated between both types of samples. The drug sensitivity...
Source: Human Cell - September 14, 2023 Category: Cytology Source Type: research
Specific downregulation of microRNA-186 induces neural stem cell self-renewal by upregulating Bmi-1/FoxG1 expression
In this study, we isolated primary NSCs from the forebrain of fetal rat for in vitro analysis. Downregulation of miRNA-186 in response to a specific miRNA inhibitor resulted in upregulation of Bmi-1 and FoxG1, while maintaining NCS self-renewal. Bmi-1 overexpression restored the maintenance of NSCs in vitro. FoxG1 was found to promote the methylation of Foxo3 promoter and inhibited Foxo3 expression. miR-186 upregulation increased the expression of Foxo3 and inhibited NSC self-renewal in the absence of Foxo3. Therefore, we propose that downregulation of miR-186 maintained NSC self-renewal in the postnatal brain by upregulat...
Source: Human Cell - September 12, 2023 Category: Cytology Source Type: research
Remodeling periodontal osteoimmune microenvironment through MAPK/NF κB phosphorylation pathway of macrophage via intelligent ROS scavenging
In conclusion, PssL-NAC regulates the osteoimmune microenvironment and protects stem cells from oxidative stress injury for bone regeneration, which provides a strategy for the treatment of periodontitis. (Source: Human Cell)
Source: Human Cell - September 11, 2023 Category: Cytology Source Type: research
Advances in combined application of dental stem cells and small-molecule drugs in regenerative medicine
AbstractTeeth are a kind of masticatory organs of special histological origin, unique to vertebrates, playing an important role in chewing, esthetics, and auxiliary pronunciation. In the past decades, with the development of tissue engineering and regenerative medicine, the studies of mesenchymal stem cells (MSCs) gradually attracted the interest of researchers. Accordingly, several types of MSCs have been successively isolated in teeth or teeth-related tissues, including dental pulp stem cells, periodontal ligament stem cells, stem cells from human exfoliated deciduous teeth, dental follicle stem cells, stem cells from ap...
Source: Human Cell - July 31, 2023 Category: Cytology Source Type: research
Functions, mechanisms, and clinical applications of lncRNA LINC00857 in cancer pathogenesis
AbstractEmerging data indicated that long noncoding RNAs (lncRNAs) are crucial players in the biological processes via regulating epigenetics, transcription, and protein translation. A novel lncRNA, LINC00857, was indicated to upregulate in several types of cancer. In addition, LINC00857 was functionally related to the modulation of the cancer-linked behaviors, including invasion, migration, proliferation, epithelial –mesenchymal transition (EMT), cell cycle, and apoptosis. The importance of LINC00857 in cancer onset and development proposed that LINC00857 has major importance in the cancer progression and may be conside...
Source: Human Cell - July 31, 2023 Category: Cytology Source Type: research
