Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus

In this study, a transposon mutagenesis approach was applied to reveal the factors involved in resistance to oxidative stress, and the biofilm formation among mutants and WT strains were further detected using crystal violet staining (CVS), scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM) to reveal potential relationship between oxidative stress and biofilm formation. Materials and Methods The Development of Mutants Library The procedure of transposon mutagenesis approach was performed as described by Zhang et al. (2018). Screening of Mutants Tolerance to Oxidative Stress For screening positive mutants tolerant to oxidative stress, overnight culture (OD600 = 0.8, v/v, 1%) was inoculated into LB broth (Luqiao, Beijing) with 1.0 mM H2O2 at 37°C for 8 h. Growth of mutants (n = 215) and WT strain were measured spectrophotometrically in 96-well culture plates (Corning, New York, NY, United States) by determining the optical density at 600 nm (OD600). Each experiment was independently done in triplicate. Growth of strains were analyzed by the statistical analysis of t-tests using OriginPro 8.5.1 software. A significant difference was defined as a p-value (p
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Source: BMJ Comments - Category: General Medicine Source Type: forums
Publication date: January 2020Source: Biometric Technology Today, Volume 2020, Issue 1Author(s): US NIST (the National Institute of Standards and Technology) has released three new biometric databases – featuring fingerprints, facial photographs and iris scans – designed to help researchers reduce the error rates of biometric systems.
Source: Biometric Technology Today - Category: Information Technology Source Type: research
Publication date: January 2020Source: Biometric Technology Today, Volume 2020, Issue 1Author(s): Jason TooleyThe use of biometrics by police forces has rarely been out of the headlines over the past year. As with many emerging technologies, it has hit significant hurdles. Recently in an unprecedented move, the UK's Information Commissioner's Office (ICO) – the government body responsible for GDPR and data privacy enforcement – launched an independent investigation into the use of facial recognition at London's King's Cross train station, after it was revealed that the general public were having their faces scan...
Source: Biometric Technology Today - Category: Information Technology Source Type: research
Publication date: January 2020Source: Biometric Technology Today, Volume 2020, Issue 1Author(s): A new study has ranked 50 countries on how extensively and invasively they use biometric surveillance, and in contrast who best regulates the technology. China is named the poorest performer of all, but perhaps surprisingly it is joined by the US in the five worst countries – while the UK is ranked among the top five best-regulated.
Source: Biometric Technology Today - Category: Information Technology Source Type: research
We have vacancies for the Editor-in-Chief positions on the Editorial Boards of Endocrine Connections and Endocrine-Related Cancer.Endocrine Connections is jointly owned by the Society for Endocrinology and the European Society of Endocrinology. It is an open access journal offering authors the highest possible visibility for their work and stimulating cross-discipline collaboration. Read the full job description.Endocrine-Related Cancer is the official flagship journal of the Society for Endocrinology and is endorsed by the European Society of Endocrinology, Endocrine Society of Australia...
Source: Society for Endocrinology - Category: Endocrinology Source Type: news
Conclusion The current level of awareness for clinical trials of cancer patients in our hospital was relatively low, even in patients who had atte nded clinical trials before. It’s necessary to improve patients’ awareness of clinical trial by promoting harmony relationship between patients and doctors, as well as by enhancing related propagation. Strengthening the adequacy and efficacy of informed consent in clinical trials also needs to b e achieved in the future. DOI: 10.3779/j.issn.1009-3419.2020.01.02
Source: Chinese Journal of Lung Cancer - Category: Cancer & Oncology Source Type: research
Conclusion T4 stage patients had worse survival in N2 group. To get a more precisely stratification, skip metastasis and number of involved lymph node stations should be considered in future N stage classification. 
 DOI: 10.3779/j.issn.1009-3419.2020.01.03
Source: Chinese Journal of Lung Cancer - Category: Cancer & Oncology Source Type: research
Conclusion The expression of TRIM59 is closely related to the prognosis of NSCLC patients, and it is an independent risk factor for NSCLC patients. DOI: 10.3779/j.issn.1009-3419.2020.01.04
Source: Chinese Journal of Lung Cancer - Category: Cancer & Oncology Source Type: research
This study mainly explores the relationship between the ASPM expression of lung adenocarcinoma and the development and prognosis of lung cancer. The aim of this study was to investigate the relationship between the expression of abnormal spindle-like microcephaly-associated protein (ASPM) in lung adenocarcinoma and the development and prognosis in lung cancer. Methods A total of 90 cases of lung adenocarcinoma tissue specimens and 90 cases of benign pulmonary lesions were collected, the expression of ASPM was detected by immunohistochemical technique, and the expression of ASPM in 12 pairs of tissues was detected by real-t...
Source: Chinese Journal of Lung Cancer - Category: Cancer & Oncology Source Type: research
Conclusion It was safety to discontinued oral antiplatelet agents before operation, with no MACE and death in perioperative period. 
 DOI: 10.3779/j.issn.1009-3419.2020.01.06
Source: Chinese Journal of Lung Cancer - Category: Cancer & Oncology Source Type: research
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