A P. falciparum NF54 Reporter Line Expressing mCherry-Luciferase in Gametocytes, Sporozoites, and Liver-Stages

This reporter line has been used to analyze liver infection in immune-deficient humanized mice engrafted with human liver tissue ((Sack et al., 2014; Flannery et al., 2018); (Foquet et al., 2018)). In multiple RMP transgenic lines the uis4 gene promoter has been used to drive expression of different transgenes in sporozoites and liver-stages, such as genes encoding mCherry, ovalbumin or human Plasmodium proteins (Combe et al., 2009; Panchal et al., 2012; Montagna et al., 2014; Hopp et al., 2015; Longley et al., 2015, 2017; Singer et al., 2015) The uis4 gene, a member of the small Plasmodium etramp gene family, is highly transcribed in sporozoites and liver-stages and encodes a parasitophorous vacuole membrane (PVM) protein that surrounds the parasite in the infected hepatocyte. Although uis4 transcripts are translationally repressed in sporozoites (Silvie et al., 2014; Silva et al., 2016), transgenes expressed under control of uis4 regulatory sequences are expressed as protein in sporozoites since uis4 translational repression in sporozoites is dependent on DNA sequences present within the uis4 open reading frame (Silvie et al., 2014; Hopp et al., 2015). In this study, we generated a transgenic Pf parasite, mCherry-luc@etramp10.3, which encodes a fusion of the proteins mCherry and luciferase (mCherry-Luc) expressed under the control of the promoter of etramp10.3 (PF3D7_1016900). We selected this promoter since etramp10.3 is related to uis4 and it also belongs to the etramp g...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

Related Links:

Authors: Bartra-More CA, Ramos-Castillo J, Mendoza-Bautista RC, Galindo-Cabello NR, Gebol-Cahuaza M, Chirinos-Palomino FJ Abstract Timely diagnosis of malaria is a strategy proposed by the World Health Organization to reduce malaria morbidity and mortality. A study was conducted to assess performance in microscopic diagnosis of malaria in the network of laboratories under the National Malaria Reference Laboratory of the National Institute of Health between 2012 and 2017. In the years of study, the laboratories obtained a rating of "acceptable" in the diagnosis of Plasmodium by 38.4%, 43.7%, 60.0%, 83.3%, ...
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research
Conclusion: The mosquito collected and mapped can be considered for transmission of malaria and filariasis in the region. Bearing in mind the results of niche modeling for vector species, more studies on vectorial capacity and resistance status to different insecticides of these species are recommended. PMID: 31803777 [PubMed]
Source: Iranian Journal of Arthropod-Borne Diseases - Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
Conclusion: Some investigations have reported An. martinius as a member of sibling species of An. sacharovi among Iranian Anopheles genus; while based on our study, there was no evidence of the presence of this species in north and northwest of Iran. PMID: 31803774 [PubMed]
Source: Iranian Journal of Arthropod-Borne Diseases - Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
Malaria is caused by unicellular apicomplexan parasites of the genus Plasmodium, which includes the major human parasite Plasmodium falciparum. The complex cycle of the malaria parasite in both mosquito and human hosts has been studied extensively. There is tight control of gene expression in each developmental stage, and at every level of gene synthesis: from RNA transcription, to its subsequent translation, and finally post-translational modifications of the resulting protein. Whole-genome sequencing of P. falciparum has laid the foundation for significant biological advances by revealing surprising genomic information. ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
While the utility of parasite genotyping for malaria elimination has been extensively documented in low to moderate transmission settings, it has been less well-characterized in holoendemic regions. High malar...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Research Source Type: research
The efficacy and safety of artemether –lumefantrine (AL) and dihydroartemisinin–piperaquine (DP) against asexual parasites population has been documented. However, the effect of these anti-malarials on sexual p...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Research Source Type: research
Abstract Twenty one chalcone derivatives were synthesized using Claisen-Schmidt condensation, their antimalarial activity against Plasmodium falciparum was determined and quantitative structure-activity relationship (QSAR) was developed. Condensation of substituted acetophenones with various aromatic aldehydes at room temperature gave chalcones in 75-96% yield. Chalcones are secondary metabolites of terrestrial plants, precursors for the biosynthesis of flavonoids and exhibit various biological activities. Antiplasmodial IC50 (half-maximal inhibitory concentration) activity of a compound against malaria parasites ...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
In this study, we dissected the response of three TCR γδ cell subsets and mucosal-associated invariant T cells in healthy volunteers immunized with PfSPZ Vaccine and challenged by CHMI using Sanaria PfSPZ Challenge. Using a flow cytometry-based unbiased analysis followed by T cell cloning, several findings were made. Whereas major ex vivo alterations were not detectable after immunization with PfSPZ Vaccine, TCR Vδ2, and mucosal-associated invariant T cells expanded after asexual blood-stage parasitemia induced by CHMI. CHMI, but not vaccination, also induced the activation of TCR Vδ1 and Vδ1-...
Source: Journal of Immunology - Category: Allergy & Immunology Authors: Tags: J Immunol Source Type: research
Resistance has developed in Plasmodium malaria parasites to every antimalarial drug in clinical use, prompting the need to characterize the pathways mediating resistance. Here, we report a framework for assessing development of resistance of Plasmodium falciparum to new antimalarial therapeutics. We investigated development of resistance by P. falciparum to the dihydroorotate dehydrogenase (DHODH) inhibitors DSM265 and DSM267 in tissue culture and in a mouse model of P. falciparum infection. We found that resistance to these drugs arose rapidly both in vitro and in vivo. We identified 13 point mutations mediating resistanc...
Source: Science Translational Medicine - Category: Biomedical Science Authors: Tags: Research Articles Source Type: research
In this study, we used focused-ion-beam-milling combined with scanning-electron-microscopy to report the 3D architecture during nuclear segregations in oocyst stage. This advanced technology allowed us to analyse the 3D details of organelle segregation inside the oocyst during sporogony formation. It was revealed that multiple nuclei were involved with several centrosomes in one germ nucleus during sporozoite budding (endopolygeny). Our high-resolution 3D analysis uncovered the endopolygeny-like nuclear architecture of Plasmodium in the definitive host. This nuclear segregation was different from that in the blood stage, a...
Source: Parasitology International - Category: Parasitology Source Type: research
More News: Biology | Biotechnology | Colombia Health | Databases & Libraries | Education | Fiji Health | Funding | Gastroschisis Repair | Genetics | Graduation | Grants | Hydrocortisone | Infectious Diseases | Insulin | Japan Health | Laboratory Medicine | Liver | Malaria | Malaria Vaccine | Malaysia Health | Microbiology | Molecular Biology | Netherlands Health | Oxford University | Papanicolaou (Pap) Smear | Parasitic Diseases | Parasitology | Pathology | Penicillin | Science | Skin | Statistics | Students | Study | UK Health | Universities & Medical Training | Urology & Nephrology | Vaccines