A P. falciparum NF54 Reporter Line Expressing mCherry-Luciferase in Gametocytes, Sporozoites, and Liver-Stages

This reporter line has been used to analyze liver infection in immune-deficient humanized mice engrafted with human liver tissue ((Sack et al., 2014; Flannery et al., 2018); (Foquet et al., 2018)). In multiple RMP transgenic lines the uis4 gene promoter has been used to drive expression of different transgenes in sporozoites and liver-stages, such as genes encoding mCherry, ovalbumin or human Plasmodium proteins (Combe et al., 2009; Panchal et al., 2012; Montagna et al., 2014; Hopp et al., 2015; Longley et al., 2015, 2017; Singer et al., 2015) The uis4 gene, a member of the small Plasmodium etramp gene family, is highly transcribed in sporozoites and liver-stages and encodes a parasitophorous vacuole membrane (PVM) protein that surrounds the parasite in the infected hepatocyte. Although uis4 transcripts are translationally repressed in sporozoites (Silvie et al., 2014; Silva et al., 2016), transgenes expressed under control of uis4 regulatory sequences are expressed as protein in sporozoites since uis4 translational repression in sporozoites is dependent on DNA sequences present within the uis4 open reading frame (Silvie et al., 2014; Hopp et al., 2015). In this study, we generated a transgenic Pf parasite, mCherry-luc@etramp10.3, which encodes a fusion of the proteins mCherry and luciferase (mCherry-Luc) expressed under the control of the promoter of etramp10.3 (PF3D7_1016900). We selected this promoter since etramp10.3 is related to uis4 and it also belongs to the etramp g...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research