Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs

This study aimed to characterize shifts in microbial community structure across the intestinal mucosa-enteric lymph node axis in order to understand the effects of two commonly used antibiotics (Linco-Spectin and Colistin sulfate; oral application). We hypothesized that these antibiotics influence GI microbiota diversity and community structure and that, as a result, the ICLN-ileum axis is altered through permeability changes and changed translocation patterns. Medicated-feed antibiotics are still heavily used, not only in the E.U. but worldwide in case of infections. In addition to the taxonomic community profiling survey with 16S rRNA gene amplicons, we sequenced the metatranscriptome of a single ICLN sample to reveal first insights into the functional aspects of the bacterial microbiome in ICLNs. Our analyses therefore fill a relevant knowledge gap with regard to the relationship between the GI tract- and the ICLN-associated bacterial microbiome. Materials and Methods Ethics Statement Animal handling and treatment were discussed and approved by the institutional ethics and animal welfare committee (ETK-03/04/2015; University of Veterinary Medicine, Vienna, Austria) in accordance with GSP guidelines and national legislation (paragraph 8ff of Law for Animal Experiments, Tierversuchsgesetz). Animals and Experimental Design Sixteen female pigs [(Large White × Landrace) × Piétrain] aged 6 months were used in this experiment. Prior to the experiment, pigs...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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ConclusionThe findings from this study have established the need to improve current education and rehabilitation programmes for patients with CHF by addressing those factors significantly influencing their adherence to diet and medication.
Source: Heart and Lung: The Journal of Acute and Critical Care - Category: Respiratory Medicine Source Type: research
Publication date: Available online 16 November 2019Source: Social Science &MedicineAuthor(s): Henry J. Whittle, Anna M. Leddy, Jacqueline Shieh, Phyllis C. Tien, Ighovwerha Ofotokun, Adaora A. Adimora, Janet M. Turan, Edward A. Frongillo, Bulent Turan, Sheri D. WeiserAbstractMaterial-need insecurities (including insecurities in basic resources such as income, food, housing, and healthcare) are widespread in the United States (US) and may be important predictors of poor health outcomes. How material-need insecurities besides food insecurity are experienced, however, remains under-researched, including how multiple mater...
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
Publication date: Available online 16 November 2019Source: Trends in Endocrinology &MetabolismAuthor(s): Carina Bodden, Anthony J. Hannan, Amy C. ReicheltGlobally, obesity has reached epidemic proportions. The rapidly increasing numbers of overweight people can be traced back to overconsumption of energy-dense, poor-quality foods as well as physical inactivity. This development has far-reaching and costly implications. Not only is obesity associated with serious physiological and psychological complications, but mounting evidence also indicates a ripple effect through generations via epigenetic changes. Parental obesit...
Source: Trends in Endocrinology and Metabolism - Category: Endocrinology Source Type: research
DiscussionHyperchloremic acidosis is associated with a non-anion gap, decrease in plasma bicarbonate and increase in plasma chloride. Rarely, as with this case, it may present with a high-anion gap. The use of bicarbonate therapy has shown improvement in cases of non-anion gap acidosis however there is very little data to support its role in high-anion gap hyperchloremic metabolic acidosis.
Source: African Journal of Emergency Medicine - Category: Emergency Medicine Source Type: research
Publication date: Available online 17 November 2019Source: Pharmacological ResearchAuthor(s): Ali Dehshahri, Milad Ashrafizadeh, Elham Ghasemipour Afshar, Abbas Pardakhty, Ali Mandegary, Reza Mohammadinejad, Gautam SethiAbstractTopoisomerase enzymes have shown unique roles in replication and transcription. These enzymes which were initially found in Escherichia coli have attracted considerable attention as target molecules for cancer therapy. Nowadays, there are several topoisomerase inhibitors in the market to treat or at least control the progression of cancer. However, significant toxicity, low solubility and poor pharm...
Source: Pharmacological Research - Category: Drugs & Pharmacology Source Type: research
ConclusionsIn contrast to Europe and North America, where R. rosea is the focal species in commerce, the trade in Rhodiola products in China is much more diverse. In the face of growing demand, both effective conservation of wild populations and cultivation are needed.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
ConclusionsThe authors concluded that oral health interventions focusing on indigenous populations have shown mixed results regarding improvements in oral health and other related outcomes.
Source: Journal of Evidence Based Dental Practice - Category: Dentistry Source Type: research
Publication date: March 2020Source: Materials Science and Engineering: C, Volume 108Author(s): Nehal Salahuddin, Mohamed Abdelwahab, Mohamed Gaber, Sahar ElneanaeyAbstractBiodegradable, biocompatible and non-toxic polymer-based nanoparticles are the novel nanotherapeutic tool which is used for adsorption and encapsulation drugs. Extended release formulation of Norfloxacin antibiotic, chemotherapeutic agent model, drug in the form of encapsulated and loaded poly (lactic acid) nanocomposites-based Titanium dioxide (PLA/TiO2) was developed. Nanocomposites were prepared using different contents (1, 3, 5 wt %) and morphologie...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 16 November 2019Source: Materials Science and Engineering: CAuthor(s): Ju-Hui Wu, Ker-Kong Chen, Chih-Yeh Chao, Yen-Hao Chang, Je-Kang DuAbstractTo instill pure Ti with an antibacterial effect, Cu was added by metallurgical alloying to produce Ti–5 wt% Cu alloy (Ti–5Cu alloy). The precipitation of the likes of Ti2Cu in a Ti–Cu alloy is one of the factors that influences its antibacterial property. However, in the present study, Ti–5Cu alloy precipitates with different microstructures were obtained by applying heat treatment at different temperatures and for diffe...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 17 November 2019Source: Journal of Membrane ScienceAuthor(s): Mustafa Arslan, Bruno A. Pulido, Suzana P. Nunes, Yusuf YagciAbstractThe modification of poly(oxindole biphenylylene) (POXI) membranes by photoinduced thiol-yne click approach is presented. Initially, a propargyl-functionalized porous poly(oxindole biphenylylene) (POXI-PG) membrane was prepared by condensation reaction of isatin and aromatic hydrocarbons with a superacid-catalyst. Subsequently, the solution and surface functionalization of POXI-PG by one-step photoinitiated thiol-yne click reaction with various thiol functional...
Source: Journal of Membrane Science - Category: Materials Science Source Type: research
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