Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs

This study aimed to characterize shifts in microbial community structure across the intestinal mucosa-enteric lymph node axis in order to understand the effects of two commonly used antibiotics (Linco-Spectin and Colistin sulfate; oral application). We hypothesized that these antibiotics influence GI microbiota diversity and community structure and that, as a result, the ICLN-ileum axis is altered through permeability changes and changed translocation patterns. Medicated-feed antibiotics are still heavily used, not only in the E.U. but worldwide in case of infections. In addition to the taxonomic community profiling survey with 16S rRNA gene amplicons, we sequenced the metatranscriptome of a single ICLN sample to reveal first insights into the functional aspects of the bacterial microbiome in ICLNs. Our analyses therefore fill a relevant knowledge gap with regard to the relationship between the GI tract- and the ICLN-associated bacterial microbiome. Materials and Methods Ethics Statement Animal handling and treatment were discussed and approved by the institutional ethics and animal welfare committee (ETK-03/04/2015; University of Veterinary Medicine, Vienna, Austria) in accordance with GSP guidelines and national legislation (paragraph 8ff of Law for Animal Experiments, Tierversuchsgesetz). Animals and Experimental Design Sixteen female pigs [(Large White × Landrace) × Piétrain] aged 6 months were used in this experiment. Prior to the experiment, pi...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research