Extraction of Extracellular Matrix in Static and Dynamic Candida Biofilms Using Cation Exchange Resin and Untargeted Analysis of Matrix Metabolites by Ultra-High-Performance Liquid Chromatography-Tandem Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS)

This study aims to investigate the efficiencies of seven methods in C. albicans biofilm EM extraction, namely vortexing plus ultrasonication (VU), formaldehyde plus vortexing and ultrasonication (Formaldehyde-VU), EDTA plus vortexing and ultrasonication (EDTA-VU), NaOH plus vortexing and ultrasonication (NaOH-VU), ethanol plus vortexing and ultrasonication (Ethanol-VU), ion exchange resin plus vortexing and ultrasonication (IER-VU)—including cation/anion-exchange resin plus vortexing and ultrasonication (CER/AER-VU)—under the static and dynamic states. Subsequently, the extracted metabolites are analyzed with the UPLC-Q-TOF-MS tool via untargeted filtration. Materials and Methods Strains and Cultivation Candida albicans SC5314 was kindly provided by Prof. Yuanying Jiang from the College of Pharmacy, the Second Military Medical University (Shanghai, China). All the stock cultures of these strains were conventionally preserved in Sabouraud agar and propagated in liquid Sabouraud medium (Hope Biotech, Co., Qingdao, China) at 37°C for 12–16 h when the cells reached the exponential phase. The revived Candida cells were collected at 5200 rpm (Leiboer Medical Devices, Beijing, China) and washed twice with sterile phosphate-buffered saline (PBS, Leagene, Beijing, China). The fungal cells were then resuspended in RPMI-1640 medium (Invitrogen, Carlsbad, CA, United States) and adjusted to a defined cell density using a hemocytometer prior to the following experi...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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This study aimed at investigating the effects of dietary protein levels on the growth and liver transcriptome in juvenile Ancherythroculter nigrocauda. Six semi-purified diets were formulated containing 25 (control), 30, 35, 40, 45, and 50% protein. Each diet was fed to three groups of 35 fish (mean initial weight: 5.86 ± 0.10 g) for 56 days. The rate of weight gain and specific growth rate increased with dietary protein levels from 25% to 40%, but remained unchanged when fed with 45 or 50% dietary protein. The feed conversion ratio was significantly influenced by the dietary protein levels, being the lowest...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 25 May 2019Source: The American Journal of SurgeryAuthor(s): Itamar Ashkenazi, Abdel-Rauf Zeina, Oded OlshaAbstractBackgroundWe determined whether increasing early imaging (in the emergency department) was associated with earlier surgery and a decrease in complicated appendicitis.MethodsRetrospective study; 3013 operations between 12/2006-12/2016.ResultsEarly imaging increased from 13.1% to 74.1%, mostly due to increasing use of ultrasound. Negative appendectomies decreased from 10.7% to 5.1% (p
Source: The American Journal of Surgery - Category: Surgery Source Type: research
This study constituted the second phase of a World Anti-Doping Agency (WADA) supported Social Science initiative.
Source: Performance Enhancement and Health - Category: Sports Medicine Source Type: research
Publication date: 1 October 2019Source: Personality and Individual Differences, Volume 148Author(s): Shuang Gao, Sander Thomaes, Wim Van Den Noortgate, Xiaochun Xie, Xiangkui Zhang, Suiping WangAbstractThe present study examined potential changes in narcissistic traits of Chinese youth from 2008 to 2017. A cross-temporal meta-analysis involving 29 independent samples (N = 14,795) found a downward temporal trend: Mean scores on the Narcissistic Personality Inventory and Narcissistic Personality Inventory for Chinese are significantly negatively associated with year of data collection, weighted by sample size (β =...
Source: Personality and Individual Differences - Category: Psychiatry & Psychology Source Type: research
Stats: state of residence: CA race: Asian (Chinese) undergrad: HYPSM major: Computer Science year: senior (taking 1 gap year) cGPA: 3.94 sGPA: 3.96 MCAT: 522 (130/131/129/132) ECs: research: my strongest EC, 2000+ hrs, 2pubs (1 first author, 1 fourth author), won some awards, researching full time during my gap year clinical volunteering: ~150hrs at free clinic, ~100hrs hrs at hospital nonclinical volunteering: started a nonprofit last year that I'll be growing in my gap year, ~200hrs... WAMC? + School List, MCAT: 522, GPA: 3.94, considering MD and MD-PhD
Source: Student Doctor Network - Category: Universities & Medical Training Authors: Tags: What Are My Chances? WAMC Medical Source Type: forums
Publication date: Available online 25 May 2019Source: European Journal of Obstetrics &Gynecology and Reproductive BiologyAuthor(s): Elisabeth Reiser, Stefanie Aust, Veronika Seebacher, Alexander Reinthaller, Hannah von Mersi, Richard Schwameis, Stephan Polterauer, Christoph Grimm, Samir Helmy-BaderAbstractObjectiveGamma-glutamyltransferase (GGT) is involved in tumor development, progression and chemotherapy resistance. The present study evaluated GGT serum levels as a preoperative predictive marker for ovarian cancer in patients with adnexal mass.Study DesignPreoperative GGT serum levels of 2235 patients with adnexal m...
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
ConclusionOur study highlights the fact that mobile genetic elements, such as plasmids, IS1216E, Tn554 and Tn558, may facilitate the horizontal transmission of optrA or poxtA.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionsOur work successfully identified the main active components in EXD, which could represent the efficacy of EXD on treating osteoporosis, and meanwhile, it also provided an effective strategy to investigate active ingredients from natural medicines, which might be helpful for drug development and application.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
ConclusionsThe reported patient and therapist perspectives highlight the need for further research into service improvement to enhance the patient experience of wearing compression garments after burns.
Source: Burns - Category: Dermatology Source Type: research
Publication date: Available online 25 May 2019Source: American Heart JournalAuthor(s): Alexander C. Fanaroff, Amit N. Vora, Anita Y. Chen, Robin Mathews, Jacob A. Udell, Matthew T. Roe, Laine Thomas, Tracy Y. WangAbstractBackgroundLittle is known about the proportion of hospitals in the United States that offer clinical trial enrollment opportunities and how patient outcomes differ between hospitals that do and do not participate in clinical trials.MethodsIn the nationwide Chest Pain - MI registry, we described the proportion of hospitals that enrolled patients with acute myocardial infarction (MI) in clinical trials from ...
Source: American Heart Journal - Category: Cardiology Source Type: research
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