Use of E-64 cysteine protease inhibitor for the recombinant protein production in Tetrahymena thermophila
This study suggests that the use of E-64 may increase the efficiency of extracellular recombinant protein production by continuously reducing extracellular cysteine protease activity during cultivation.PMID:38703600 | DOI:10.1016/j.ejop.2024.126085 (Source: European Journal of Protistology)
Source: European Journal of Protistology - May 4, 2024 Category: Microbiology Authors: Yeliz D ündar Orhan Ay ça Fulya Üstüntanır Dede Şeyma Duran Muhittin Arslanyolu Source Type: research
Effects of fasting and environmental factors on appetite regulators in pond loach Misgurnus anguillicaudatus
In this study, we examined the effects of fasting, as well as increased temperature and decreased oxygen levels on food intake and transcript levels of appetite regulators. Fasted fish had lower blood glucose levels, and lower expression levels of intestine CCK and PYY, and brain CART1, but had higher levels of brain orexin and ghrelin than fed fish. Fish held at 30 °C had higher food intake, glucose levels, and mRNA levels of intestine CCK and PYY, and brain CART2, but lower brain orexin levels than fish at 20 °C. Fish held at low oxygen levels had a lower food intake, higher intestine CCKa and ghrelin, and brain orexin...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - May 4, 2024 Category: Physiology Authors: Jannik Kuhn Annika Lindstrom Helene Volkoff Source Type: research
The effects of the predictability of acclimatory temperature on the growth and thermal tolerance of juvenile Spinibarbus sinensis
Comp Biochem Physiol A Mol Integr Physiol. 2024 May 2:111652. doi: 10.1016/j.cbpa.2024.111652. Online ahead of print.ABSTRACTHeated effluent injection, cold hypolimnetic water inputs from dams, and extreme weather events can lead to unpredictable temperature fluctuations in natural waters, impacting fish performance and fitness. We hypothesized that fish exposed to such unpredictable fluctuations would exhibit weaker growth and enhanced thermal tolerance compared to predictable conditions. Qingbo (Spinibarbus sinensis) was selected as the experimental subject in this study. The qingbo were divided into a constant temperatu...
Source: Comparative Biochemistry and Physiology. Part A, Molecular and integrative physiology. - May 4, 2024 Category: Physiology Authors: Cheng Fu Ke-Ying Zhou Yue Hu Yong-Fei Zhang Shi-Jian Fu Source Type: research
CG9920 is necessary for mitochondrial morphogenesis and individualization during spermatogenesis in Drosophila melanogaster
In this study, we found that CG9920 was highly expressed in fly testes. CG9920 knockdown in fly testes caused male infertility with no mature sperms in seminal vesicles. Immunofluorescence staining showed that depletion of CG9920 resulted in scattered spermatid nuclear bundles, fewer elongation cones that did not migrate to the anterior region of the testis, and almost no individualization complexes. Transmission electron microscopy revealed that CG9920 knockdown severely disrupted mitochondrial morphogenesis during spermatogenesis. Notably, we found that CG9920 might not directly interact with Ocn, but rather was inhibite...
Source: Developmental Biology - May 4, 2024 Category: Biology Authors: Chao Li Yue Ren Meng-Yan Chen Qian Wang Zhen He Yu-Feng Wang Source Type: research
Paeoniflorin exerts anti-PTSD effects in adult rats by modulating hippocampus and amygdala histone acetylation modifications in response to early life stress
This study found that ELS lead to a series of PTSD-like behaviors in adulthood and caused imbalance of HATs/HDACs ratio in the hippocampus and amygdala, which confirms that ELS is an important risk factor for the development of PTSD in adulthood. In addition, paeoniflorin may improve ELS-induced PTSD-like behaviors and reduce the susceptibility of ELS rats to develop PTSD in adulthood by modulating the HATs/HDACs ratio in the hippocampus and amygdala.PMID:38703807 | DOI:10.1016/j.cbi.2024.111035 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - May 4, 2024 Category: Molecular Biology Authors: Hanfang Xu Tiange Zhang Ling Li Yue Qu Lanxin Li Yuqi Yan Lili Wu Can Yan Source Type: research
Interaction of dinuclear Co(III) cylinders with higher-order DNA structures
This study was motivated by earlier results demonstrating that both Co(III) cylinders exhibit potent and selective activity against cancer cells, accumulate in the nucleus of cancer cells, and prove to be efficient DNA binders. The results show that bigger cylinder rac-2 stabilizes all DNA structures, while smaller cylinder meso-1 stabilizes just the Y-shaped three-way junctions. Collectively, the results of this study suggest that the stabilization of alternative DNA structures by Co(III) cylinders investigated in this work might contribute to the mechanism of their biological activity.PMID:38703805 | DOI:10.1016/j.cbi.20...
Source: Chemico-Biological Interactions - May 4, 2024 Category: Molecular Biology Authors: Jaroslav Malina James D Crowley Viktor Brabec Source Type: research
< em > In Vitro < /em > Biocompatibility and Endothelial Permeability of Branched Polyglycidols Generated by Ring-Opening Polymerization of Glycidol with B(C < sub > 6 < /sub > F < sub > 5 < /sub > ) < sub > 3 < /sub > under Dry and Wet Conditions
This study explores the biomedical promise of bcPG, particularly its ability to cross the blood-brain barrier (BBB). We evaluate in vitro biocompatibility, endothelial permeability, and formation of branched linear PG (blPG) as topological impurities in the presence of water. Small angle X-ray scattering in solution revealed a fractal dimension of approximately two for bcPG and the mixture bc+blPG, suggesting random branching. Comparisons of cytotoxicity and endothelial permeability between bcPG, bc+blPG, and HPG in a BBB model using hCMEC/D3 cells showed different biocompatibility profiles and higher endothelial permeabil...
Source: Biomacromolecules - May 4, 2024 Category: Biochemistry Authors: Carlo Andrea Pagnacco Marcelo H Kravicz Francesco Saverio Sica Veronica Fontanini Estibaliz Gonz ález de San Román Reidar Lund Francesca Re Fabienne Barroso-Bujans Source Type: research
Detecting normal and cancer skin cells via glycosylation and adhesion signatures: A path to enhanced microfluidic phenotyping
Biosens Bioelectron. 2024 Apr 25;258:116337. doi: 10.1016/j.bios.2024.116337. Online ahead of print.ABSTRACTRecruiting circulating cells based on interactions between surface receptors and corresponding ligands holds promise for capturing cells with specific adhesive properties. Our study investigates the adhesion of skin cells to specific lectins, particularly focusing on advancements in lectin-based biosensors with diagnostic potential. We explore whether we can successfully capture normal skin (melanocytes and keratinocytes) and melanoma (WM35, WM115, WM266-4) cells in a low-shear flow environment by coating surfaces wi...
Source: Biosensors and Bioelectronics - May 4, 2024 Category: Biotechnology Authors: Renata Szydlak Marcin Luty Victorien E Prot Ingrid H Øvreeide Joanna Zem ła Bj ørn T Stokke Ma łgorzata Lekka Source Type: research
Evaluation of sustainable hydrogen production technologies on an industrial scale using comparative analysis of decision-making methods
Environ Sci Pollut Res Int. 2024 May 4. doi: 10.1007/s11356-024-33535-z. Online ahead of print.ABSTRACTDue to its various advantages in different industrial fields, hydrogen can provide energy based on sustainability goals and recreates a critical function in the economy of countries. In this regard, evaluating hydrogen production technologies on an industrial scale is necessary for industrial development and economic growth. Therefore, this study proposes a comprehensive, integrated framework of hybrid fuzzy decision-making for assessing hydrogen production technologies in Iran. In addition to considering sustainability f...
Source: Environmental Science and Pollution Research International - May 4, 2024 Category: Environmental Health Authors: Seyyed Jalaladdin Hosseini Dehshiri Ali Mostafaeipour Ttu Le Ali Rezaeian Sabagh Source Type: research
Bioaccumulation of arsenic in fish (Labeo rohita) in presence of periphyton: ameliorative effect on oxidative stress, physiological condition, immune response and risk assessment
Environ Sci Pollut Res Int. 2024 May 4. doi: 10.1007/s11356-024-33302-0. Online ahead of print.ABSTRACTThe present study explores the use of periphyton to ameliorate toxic properties of arsenic (As) to Labeo rohita and also assesses the human food safety aspects. Fish were introduced to arsenite [As(III)] contaminated water (0.3 and 3 mg/L) along with periphyton. Biochemical, physiological and immunological parameters, including gene expression, were assessed after 30 days of exposure. Periphyton incorporation significantly improved (p < 0.05) the adverse effects of As on respiration, NH3 excretion and brain AChE activi...
Source: Environmental Science and Pollution Research International - May 4, 2024 Category: Environmental Health Authors: Vanniaraj Santhana Kumar Dhruba Jyoti Sarkar Basanta Kumar Das Srikanta Samanta Gayatri Tripathi Soma Das Sarkar Anjon Talukder Source Type: research
Investigation of the effects and mechanisms of manganese-based NMs on rice growth
This study investigated the physiological effects of manganese dioxide (MnO2) and manganese tetroxide (Mn3O4) NMs on inter-root exposure (0-500 mg/L) of hydroponically grown rice. The results showed that on inter-root exposure, 50 mg/L Mn-based NMs promoted the uptake of mineral elements and enhanced the enzymatic activities of antioxidant systems (CAT and SOD) in rice, whereas 500 mg/L Mn3O4 NMs disrupted the mineral element homeostasis and led to phytotoxicity. The promotion effect of MnO2 NMs was better, firstly because MnO2 NMs treatment had lower Mn content in the plant than Mn3O4 NMs. In addition, MnO2 NMs are more t...
Source: Environmental Science and Pollution Research International - May 4, 2024 Category: Environmental Health Authors: Yaqi Jiang Yi Sun Peng Zhang Pingfan Zhou Qibin Wang Guikai Zhu Weichen Zhao Yuanbo Li Quanlong Wang Yukui Rui Source Type: research
Environmental perchlorate, thiocyanate, and nitrate exposures and bone mineral density: a national cross-sectional study in the US adults
This study aimed to estimate the associations of individual and concurrent exposure of the three chemicals with adult BMD. Based on National Health and Nutrition Examination Survey (NHANES, 2011-2018), 1618 non-pregnant adults (age ≥ 20 years and 47.0% female) were included in this study. Survey-weighted linear regression models were used to estimate individual urinary perchlorate, thiocyanate, and nitrate concentrations with lumbar spine BMD and total BMD in adults. Then, weighted quantile sum (WQS) regression and Bayesian kernel machine regression (BKMR) models were conducted to evaluate associations of co-occurrence o...
Source: Environmental Science and Pollution Research International - May 4, 2024 Category: Environmental Health Authors: Juxiao Li Bohai Du Yuhan Wang Jiahuang Qiu Ming Shi Muhong Wei Li Li Source Type: research
Two-layer elastic models for single-yeast compressibility with flat microlevers
Eur Biophys J. 2024 May 4. doi: 10.1007/s00249-024-01710-2. Online ahead of print.ABSTRACTUnicellular organisms such as yeast can survive in very different environments, thanks to a polysaccharide wall that reinforces their extracellular membrane. This wall is not a static structure, as it is expected to be dynamically remodeled according to growth stage, division cycle, environmental osmotic pressure and ageing. It is therefore of great interest to study the mechanics of these organisms, but they are more difficult to study than other mammalian cells, in particular because of their small size (radius of a few microns) and...
Source: European Biophysics Journal : EBJ - May 4, 2024 Category: Physics Authors: L Delmarre E Hart é A Devin P Argoul F Argoul Source Type: research
PLD2 deficiency alleviates endothelial glycocalyx degradation in LPS-induced ARDS/ALI
Biochem Biophys Res Commun. 2024 Apr 26;716:150019. doi: 10.1016/j.bbrc.2024.150019. Online ahead of print.ABSTRACT- Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a life-threatening condition marked by severe lung inflammation and increased lung endothelial barrier permeability. Endothelial glycocalyx deterioration is the primary factor of vascular permeability changes in ARDS/ALI. Although previous studies have shown that phospholipase D2 (PLD2) is closely related to the onset and progression of ARDS/ALI, its role and mechanism in the damage of endothelial cell glycocalyx remains unclear. We used L...
Source: Biochemical and Biophysical Research communications - May 4, 2024 Category: Biochemistry Authors: Guiqing Kong Dongxiao Li Xiangyong Liu Jiali Feng Fangyu Ning Xiao Huang Boyang Qi Jianyu Qu Xiaozhi Wang Source Type: research
Characterizing the voxel-based approaches in radioembolization dosimetry with reDoseMC
CONCLUSIONS: reDoseMC was developed and validated for radiopharmaceutical dosimetry. The accuracy of voxel-based dosimetry was found to vary widely with changes in image resolutions, voxel sizes, chosen target volumes, and tissue material; hence, the standardization of dosimetry protocols was found to be of great importance for comparable dosimetry analysis.PMID:38703394 | DOI:10.1002/mp.17054 (Source: Health Physics)
Source: Health Physics - May 4, 2024 Category: Physics Authors: Taehyung Peter Kim Shirin A Enger Source Type: research
