Polymyxin B and polymyxin E induce anaphylactoid response through mediation of Mas-related G protein–coupled receptor X2
Publication date: Available online 25 May 2019Source: Chemico-Biological InteractionsAuthor(s): Yingzhuan Zhan, Nan Ma, Rui Liu, Nan Wang, Tao Zhang, Langchong HeAbstractPolymyxin B (PMB) and polymyxin E (PME) are cyclic, peptide antibiotics which derived from various species of Paenibacillus (Bacillus) polymyxa. They are decapeptide antibiotics with an antimicrobial spectrum that includes Gram-negative bacteria, and reused as therapeutic agents due to the emergence of multidrug-resistant (MDR) Gram-positive bacteria. PMB or PME-induced anaphylactoid reactions in the clinic have been documented. However, the mechanism unde...
Source: Chemico Biological Interactions - May 25, 2019 Category: Biochemistry Source Type: research

Association of status of acetylcholinesterase and ACHE gene 3’ UTR variants (rs17228602, rs17228616) with drug addiction vulnerability in pakistani population
Publication date: Available online 23 May 2019Source: Chemico-Biological InteractionsAuthor(s): Tahira Javed, Rabia Habib, Sania Ghafoor, Baseerat Ruman, Sliha Awan, Leonel J.M. Ntepe, Sajida Batool, Syed M. NurulainAbstractSubstance addiction is a chronic, relapsing mental disorder Characterized by compulsive drug seeking, and loss of control over drug intake and relapse after prolonged abstinence. Genetics has been shown to contribute towards an individual's vulnerability to addiction. Acetylecholine (ACh), a cholinergic neurotransmitter hydrolyzed by acetylcholinesterase (AChE), is an essential neurotransmitter and neur...
Source: Chemico Biological Interactions - May 25, 2019 Category: Biochemistry Source Type: research

Zolmitriptan attenuates hepatocellular carcinoma via activation of caspase mediated apoptosis
Publication date: Available online 23 May 2019Source: Chemico-Biological InteractionsAuthor(s): Vimal Maurya, Pranesh Kumar, Sreemoyee Chakraborti, Ashok K. Singh, Archana S. Bhadauria, Umesh Kumar, Dinesh Kumar, Arnab Pramanik, Biswajit Saha, Venkatesh Kumar R, Sunil Babu Gosipatala, Bolay Bhattachariya, Biswanath Maity, Sudipta SahaAbstractA preclinical study using DEN-induced HCC rat model was attempted to evaluate the antitumor potential of zolmitriptan (ZOL). The molecular insights were investigated using ELISA, qRT-PCR and Western blot techniques. The result confirmed that the HCC condition was developed in response ...
Source: Chemico Biological Interactions - May 25, 2019 Category: Biochemistry Source Type: research

Human plasma-derived butyrylcholinesterase is behaviorally safe and effective in cynomolgus macaques (Macaca fascicularis) challenged with soman
Publication date: Available online 24 May 2019Source: Chemico-Biological InteractionsAuthor(s): Todd M. MyersAbstractOrganophosphorus compounds (OP) pose a significant threat. Administration of human butyrylcholinesterase (HuBChE) may reduce or prevent OP toxicity. Thus, we evaluated the safety and efficacy of HuBChE in monkeys using sensitive neurobehavioral tests while concurrently characterizing absorption and elimination in the presence and absence of high-dose soman exposure to predict time course and degree of protection. Eight young adult male cynomolgus macaques were trained on two distinct automated tests of neuro...
Source: Chemico Biological Interactions - May 25, 2019 Category: Biochemistry Source Type: research

CYP3A suppression during diet-induced nonalcoholic fatty liver disease is independent of PXR regulation
Publication date: Available online 24 May 2019Source: Chemico-Biological InteractionsAuthor(s): Hang Zeng, Yiming Lin, Jiande Gong, Sisi Lin, Jianguo Gao, Chunxiao Li, Zemin Feng, Hong Zhang, Jie Zhang, Youming Li, Chaohui YuAbstractCytochrome P450 3A (CYP3A) activity is inhibited, and its expression is suppressed during many diseases, including nonalcoholic fatty liver disease (NAFLD). However, the mechanism is controversial. Here, we report that PXR may not take part in the downregulation of CYP3A during NAFLD. Hepatic CYP3A11 (major subtype of mouse CYP3A) mRNA and protein expression was significantly decreased in both ...
Source: Chemico Biological Interactions - May 25, 2019 Category: Biochemistry Source Type: research

Molecular modelling studies on the interactions of 7-methoxytacrine-4-pyridinealdoxime with VX-inhibited human acetylcholinesterase. A near attack approach to assess different spacer-lengths
Publication date: Available online 21 May 2019Source: Chemico-Biological InteractionsAuthor(s): Jorge Alberto Valle da Silva, Eugenie Nepovimova, Teodorico Castro Ramalho, Kamil Kuca, Tanos Celmar Costa FrançaAbstractThe novel prophylactic agent 7-methoxytacrine-4-pyridinealdoxime is a hybrid compound that was formerly designed to keep acetylcholinesterase resistant to organophosphates inhibitors and to reactivate that enzyme in the case of intoxication by such inhibitors. In rational design, a 5-carbon length-spacer hybrid compound was synthesized to evaluate its inhibitory and reactivation capabilities. In this wo...
Source: Chemico Biological Interactions - May 21, 2019 Category: Biochemistry Source Type: research

A review on protective role of genistein against oxidative stress in diabetes and related complications
The objective of treatment for DM is to inhibit mortality and difficulties through normalizing blood glucose stage. Genistein, a naturally available soy isoflavone, is accounted for to have various medical advantages credited to numerous natural capacities. In the course of recent years, various examinations have shown that genistein has hostile to diabetic impacts, specifically, direct consequences for β-cell expansion, glucose-triggered insulin discharge, and safety towards apoptosis, unbiased of its functions as an estrogen receptor agonist, cancer prevention agent, or tyrosine kinase inhibitor. The present evaluat...
Source: Chemico Biological Interactions - May 21, 2019 Category: Biochemistry Source Type: research

Matrine induces apoptosis and autophagy of glioma cell line U251 by regulation of circRNA-104075/BCL-9
ConclusionTaken together, the present findings suggested that matrine induced apoptosis and autophagy through down-regulating circ-104075 and Bcl-9 expression via inhibition of PI3K/AKT and Wnt-β-catenin pathways in glioma cells. The present study provides a foundation for further preclinical and clinical evaluations of matrine as a glioma therapy. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - May 19, 2019 Category: Biochemistry Source Type: research

EPA adopts LNT: New historical perspectives
Publication date: Available online 17 May 2019Source: Chemico-Biological InteractionsAuthor(s): Edward J. CalabreseAbstractThis paper provides an historical assessment of how the linear non-threshold (LNT) model became adopted as policy by the United States Environmental Protection Agency (US EPA) in 1975 [1] and how prior United States National Academy of Sciences (US NAS) radiation advisory panels may have affected this EPA decision. The paper highlights a generally unrecognized set of recommendations of the 1960 Biological Effect of Atomic Radiation [2] Genetics and Medical/Pathology Panels that did not support LNT for ...
Source: Chemico Biological Interactions - May 18, 2019 Category: Biochemistry Source Type: research

Methylglyoxal activates osteoclasts through JNK pathway leading to osteoporosis
In this study, therefore, the underlying mechanism of methylglyoxal on osteoporosis was studied using both animal and cell models.In the animal model, rats were treated with either methylglyoxal or saline as control. In the cell model, the macrophage RAW264.7 was treated with methylglyoxal or vehicle control. Following the treatment, animal samples were harvested for micro-CT and real-time polymerase chain reaction analyses. Cell samples were harvested for MTT assay, RT-PCR, and Western Blotting analyses.In both animals and cell cultures, methylglyoxal was shown to induce osteoclastogenesis by increased gene expression of ...
Source: Chemico Biological Interactions - May 18, 2019 Category: Biochemistry Source Type: research

In vitro and in vivo studies of the metabolic activation of chelidonine
This study generated data that may be useful in understanding possible mechanisms of CHE-induced P450 inhibition. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - May 18, 2019 Category: Biochemistry Source Type: research

Wogonin pre-treatment attenuates cisplatin-induced nephrotoxicity in rats: Impact on PPAR-γ, inflammation, apoptosis and Wnt/β-catenin pathway
This study was designed to explore the possible nephroprotective impact of wogonin, a forceful anti-oxidant, anti-inflammatory, and anti-tumor agent, in a rat model of cisplatin-induced renal injury. The potential nephroprotective mechanisms were additionally investigated. Wogonin was given at a dose of 40 mg/kg. Acute nephrotoxicity was indicated by a significant rise in BUN, and serum creatinine levels in cisplatin-injected rats. Also, cisplatin enhanced the lipid peroxidation, diminished GSH, catalase, and PPAR-γ levels. Additionally, cisplatin-injected rats showed a significant rise in tissue levels of IL-1&bet...
Source: Chemico Biological Interactions - May 18, 2019 Category: Biochemistry Source Type: research

Protective effect of morin on doxorubicin-induced hepatorenal toxicity in rats
Publication date: Available online 14 May 2019Source: Chemico-Biological InteractionsAuthor(s): Müslüm Kuzu, Serkan Yildirim, Fatih Mehmet Kandemir, Sefa Küçükler, Cüneyt Çağlayan, Erdinç Türk, Muhammet Bahaeddin DörtbudakAbstractAlthough Doxorubicin (DOX) is a widespread drug used in the treatment of cancer, its clinical use is restricted due to its common side effects. In addition, administrating DOX with an antioxidant has recently become a new strategy in preventing the side effects of DOX. The protective effects of morin, a natural flavonoid, against DOX-induced...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Assessment of ionizable, zwitterionic oximes as reactivating antidotal agents for organophosphate exposure
Publication date: Available online 14 May 2019Source: Chemico-Biological InteractionsAuthor(s): Palmer Taylor, Shyong Yan-Jye, Jeremiah Momper, William Hou, Gisela Andrea Camacho-Hernandez, Zoran Radic, Yvonne Rosenberg, Zrinka Kovarik, Rakesh Sit, K. Barry SharplessAbstractSince the development in the 1950's of 2-PAM (Pralidoxime), an antidote that reactivates organophosphate conjugated acetylcholinesterase in target tissues upon pesticide or nerve agent exposure, improvements in antidotal therapy have largely involved congeneric pyridinium aldoximes. Despite seminal advances in detailing the structures of the cholinester...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure on acetylcholinesterase during myogenic differentiation of contractile rat primary skeletal muscle cells
Publication date: Available online 14 May 2019Source: Chemico-Biological InteractionsAuthor(s): Yali Luo, Heidi Qunhui Xie, Yangsheng Chen, Yingjie Xia, Rui Sha, Yiyun Liu, Yongchao Ma, Tong Xu, Li Xu, Karl Wah-Keung Tsim, Bin ZhaoAbstractEmerging data indicate that prenatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) could interfere with myogenic differentiation in vivo. Acetylcholinesterase (EC3.1.1.7; AChE), an enzyme critical for cholinergic neurotransmission, is abundantly expressed in neurons and mature myotubes, and we recently found that muscle AChE expression was suppressed in parallel with the inhibiti...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Surface screening, molecular modeling and in vitro studies on the interactions of aflatoxin M1 and human enzymes acetyl- and butyrylcholinesterase
Publication date: Available online 14 May 2019Source: Chemico-Biological InteractionsAuthor(s): Joyce S.F.D. de Almeida, Samir F.de A. Cavalcante, Rafael Dolezal, Kamil Kuca, Kamil Musilek, Daniel Jun, Tanos C.C. FrançaAbstractAflatoxin M1 (AFM1) is a mycotoxin produced by Aspergillus fungi and found in contaminated milk, breastfeed and dairy products, being highly toxic and carcinogenic to humans and other mammalian species. It is also produced in the human body as a metabolite of aflatoxin B1 (AFB1), one of the most toxic natural products known. Previous studies have shown that AFM1 is a potential inhibitor of the...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

An evolutionary perspective on the first disulfide bond in members of the cholinesterase-carboxylesterase (COesterase) family: Possible outcomes for cholinesterase expression in prokaryotes
Publication date: Available online 14 May 2019Source: Chemico-Biological InteractionsAuthor(s): Arnaud Chatonnet, Xavier Brazzolotto, Thierry Hotelier, Nicolas Lenfant, Pascale Marchot, Yves BourneAbstractWithin the alpha/beta hydrolase fold superfamily of proteins, the COesterase group (carboxylesterase type B, block C, cholinesterases…) diverged from the other groups through simultaneous integration of an N-terminal, first disulfide bond and a significant increase in the protein mean size. This first disulfide bond ties a large Cys loop, which in the cholinesterases is named the omega loop and forms the upper part...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Overview of novel multifunctional agents based on conjugates of γ-carbolines, carbazoles, tetrahydrocarbazoles, phenothiazines, and aminoadamantanes for treatment of Alzheimer's disease
Publication date: Available online 14 May 2019Source: Chemico-Biological InteractionsAuthor(s): Galina F. Makhaeva, Elena F. Shevtsova, Natalia P. Boltneva, Sofya V. Lushchekina, Nadezhda V. Kovaleva, Elena V. Rudakova, Sergey O. Bachurin, Rudy J. RichardsonAbstractAlzheimer's disease (AD) is a multifactorial neurodegenerative process whose effective treatment will require drugs that can act simultaneously on multiple pathogenic targets. Here, we present an overview of our previous multi-target studies of five groups of novel hybrid structures that combine, through spacers, five pharmacophores that have been found promisin...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

The effect of boletus polysaccharides on diabetic hepatopathy in rats
The objective of this work was to investigate the effect of boletus polysaccharide (BPS) on diabetic hepatopathy in streptozotocin (STZ)–induced type 2 diabetes mellitus (T2DM) rats for the first time. The rats were fed with high-fat diet (HFD) for 4 weeks and induced with STZ by a single intraperitoneal injection to develop T2DM model. The HFD was given continually for another 4 weeks after diabetes induction, following the drugs of BPS (400 mg/kg bw/day) infused to stomach of rats once a day. After the administration, blood was drawn from the posterior orbital venous plexus of the inner canthus and the rats wer...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Structural aspects of 4-aminoquinolines as reversible inhibitors of human acetylcholinesterase and butyrylcholinesterase
Publication date: Available online 15 May 2019Source: Chemico-Biological InteractionsAuthor(s): Anita Bosak, Dejan M. Opsenica, Goran Šinko, Matija Zlatar, Zrinka KovarikAbstractEight derivatives of 4-aminoquinolines differing in the substituents attached to the C(4)-amino group and C(7) were synthesised and tested as inhibitors of human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Both enzymes were inhibited by all of the compounds with inhibition constants (Ki) ranging from 0.50 to 50 μM exhibiting slight selectivity toward AChE over BChE. The most potent inhibitors of AChE were compounds wit...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells
Publication date: Available online 15 May 2019Source: Chemico-Biological InteractionsAuthor(s): Yu Wang, Cuiqin Zhang, Zhenyu Shen, Ruirui Kou, Keqin Xie, Fuyong SongAbstractTri-ortho-cresyl phosphate (TOCP) is a typical organophosphorus compound that can cause organophosphate-induced delayed neuropathy (OPIDN), which is pathologically characterized by axonal degeneration. Nowadays, mitochondrial dysfunction is regarded as a potential mechanism contributing to OPIDN progress. Mitophagy, a selective type of autophagy, is required to segregate damaged mitochondria from healthy mitochondrial networks and deliver them to lysos...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

The evolution of phosphotriesterase for decontamination and detoxification of organophosphorus chemical warfare agents
Publication date: Available online 15 May 2019Source: Chemico-Biological InteractionsAuthor(s): Andrew N. Bigley, Frank M. RaushelAbstractThe organophosphorus chemical warfare agents were initially synthesized in the 1930's and are some of the most toxic compounds ever discovered. The standard means of decontamination are either harsh chemical hydrolysis or high temperature incineration. Given the continued use of chemical warfare agents there are ongoing efforts to develop gentle environmentally friendly means of decontamination and medical counter measures to chemical warfare agent intoxication. Enzymatic decontamination...
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

Hesperidin, a citrus flavanone glycoside, accelerates the gastric healing process of acetic acid-induced ulcer in rats
In conclusion, the flavonoid hesperidin revealed a gastric healing activity in the ulcerated mucosa, an effect that showed to be associated with the reduction of oxidative damage at ulcer site, due to the reduction of the neutrophil migration and the strengthening of the mucus barrier next to the mucosa. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - May 15, 2019 Category: Biochemistry Source Type: research

A comprehensive review on hepatoprotective and nephroprotective activities of chrysin against various drugs and toxic agents
Publication date: Available online 11 May 2019Source: Chemico-Biological InteractionsAuthor(s): Ravindra Babu Pingili, A. Krishnamanjari Pawar, Siva R. Challa, Tanvija Kodali, Sirisha Koppula, Vyshnavi ToletiAbstractChrysin belongs to the flavonoids and has been used as traditional medicine from ancient and has been reported to exhibit a wide range of pharmacological properties. The biochemical and molecular mechanisms involved in the hepato- and nephroprotective activities of chrysin were discussed in this review. Chrysin exhibited hepatoprotective activity against 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin, carbon tetrach...
Source: Chemico Biological Interactions - May 11, 2019 Category: Biochemistry Source Type: research

Alpinoid C analog inhibits angiogenesis and induces apoptosis in COLO205 cell line
Publication date: Available online 6 May 2019Source: Chemico-Biological InteractionsAuthor(s): Loka Reddy Velatooru, V. Sridhar, Vasudevarao Penugurti, S. Purushotham ReddyAbstractDiarylheptanoids display an array of biological and pharmacological properties. We previously reported the synthesis of a diarylheptanoid Alpinoid c and a series of its derivatives, evaluated their cytotoxicity against various human cancer cells. We found some of these derivatives were significantly more potent than Alpinoid c in preventing the proliferation of various cancer cell lines. Among these, (S, E)-1-(3, 4 dimethoxy phenyl)-6-hydroxy-7-p...
Source: Chemico Biological Interactions - May 7, 2019 Category: Biochemistry Source Type: research

Haplotypes of butyrylcholinesterase K-variant and their influence on the enzyme activity
We present three haplotypes of BChE-K variant, two of them coexist in strong linkage disequilibrium with alterations in 5′UTR (rs1126680), intron 2 (rs55781031) or in exon 2 (rs1799807). We demonstrate a negative role of these alterations on enzyme activity. By oneself BCHE-K (with no other alterations in BCHE gene) haplotype exhibits wild type enzyme activity.Based on our previous and presented results we conclude that SNPs localized outside the coding sequence, in 5′UTR or/and in intron 2 of BCHE gene, but not solely in K-variant alteration (p.A539T) itself, are responsible for reduced enzyme activity. (Sourc...
Source: Chemico Biological Interactions - May 7, 2019 Category: Biochemistry Source Type: research

Phloretin loaded chitosan nanoparticles enhance the antioxidants and apoptotic mechanisms in DMBA induced experimental carcinogenesis
Publication date: Available online 7 May 2019Source: Chemico-Biological InteractionsAuthor(s): Mariadoss Arokia Vijaya Anand, Vinayagam Ramachandran, Baojun Xu, Venkatachalam Karthikumar, Vijayalakshmi Sankaran, Shalini Vijayakumar, Bakthavatsalam Sandya Rani, A.Mohamed Sadiq, Ernest DavidAbstractThe main aim of this study was to investigate the effects of phloretin loaded chitosan nanoparticles (PhCsNPs) on 7,12-dimethylbenz[a]anthracene (DMBA) induced experimental cancer in hamsters. Oral squamous cell carcinoma (OSCC) was induced in male golden Syrian hamsters by painting with 0.5% DMBA three times a week for 14 weeks. ...
Source: Chemico Biological Interactions - May 7, 2019 Category: Biochemistry Source Type: research

Mitochondrial and lysosomal dysfunction induced by the novel metal-insecticide [Mg(hesp)2(phen)] in the zebrafish (Danio rerio) hepatocyte cell line (ZF-L)
Publication date: Available online 7 May 2019Source: Chemico-Biological InteractionsAuthor(s): Marina Marques Bonomo, João Batista Fernandes, Rose Maria Carlos, Marisa Narciso FernandesAbstractThe development of novel agrochemical compounds to reduce the use of pesticides with high ecological impact is urgently needed. A complex of Mg with two flavonoids hesperidin and phenanthroline [Mg(hesp)2(phen)], referred to as MgHP, results in high insecticidal activity against urban, agricultural and forest insect pests. In vitro cytotoxicity biomarkers were used to assess the mechanism of action MgHP on fish cells, as this ...
Source: Chemico Biological Interactions - May 7, 2019 Category: Biochemistry Source Type: research

Insights into the cardioprotective properties of n-3 PUFAs against ischemic heart disease via modulation of the innate immune system
Publication date: Available online 5 May 2019Source: Chemico-Biological InteractionsAuthor(s): Ahmed M. Darwesh, Deanna K. Sosnowski, Tim YT. Lee, Hedieh Keshavarz-Bahaghighat, John M. SeubertAbstractIschemic heart diseases (IHD) is a major cause of cardiovascular death and disability worldwide. IHD is characterized by an imbalance between cardiac oxygen supply and demand predominantly due to obstruction of coronary arteries. Activation of the innate immune system and the consequent inflammatory response is an important contributor in the pathogenesis of IHD. An excessive and uncontrolled inflammatory response contributes ...
Source: Chemico Biological Interactions - May 7, 2019 Category: Biochemistry Source Type: research

Human mitochondrial carnitine acylcarnitine carrier: Molecular target of dietary bioactive polyphenols from sweet cherry (Prunus avium)
Publication date: Available online 4 May 2019Source: Chemico-Biological InteractionsAuthor(s): Console Lara, Giangregorio Nicola, Cellamare Saverio, Bolognino Isabella, Palasciano Marino, Indiveri Cesare, Incampo Giovanna, Campana Sabrina, Tonazzi AnnamariaAbstractThe effect of polyphenols, recognized as the principal antioxidant and beneficial molecules introduced with the diet, extracted from sweet cherry (Prunus avium) on the recombinant human mitochondrial carnitine/acylcarnitine transporter (CACT) has been studied in proteoliposomes. CACT transport activity, which was strongly impaired after oxidation by atmospheric O...
Source: Chemico Biological Interactions - May 5, 2019 Category: Biochemistry Source Type: research

Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy
In conclusion, naringenin has a therapeutic effect on DN, which may be, at least partly, related to the activation of CYP4A-20-HETE and the up-regulation of PPARs.Graphical abstract (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - May 5, 2019 Category: Biochemistry Source Type: research

Overexpression of glutathione peroxidase-1 attenuates cocaine-induced reproductive dysfunction in male mice by inhibiting nuclear factor κB
Publication date: Available online 3 May 2019Source: Chemico-Biological InteractionsAuthor(s): Huynh Nhu Mai, Yoon Hee Chung, Eun-Joo Shin, Ji Hoon Jeong, Tae Woo Jung, Naveen Sharma, Xin Gen Lei, Seung-Yeol Nah, Choon-Gon Jang, Dae-Joong Kim, Boo-Keun Yang, Hyoung-Chun KimAbstractSince reproductive toxicity is associated with oxidative stress, nuclear factor κB (NFκB), a redox-sensitive transcription factor, may be involved in the reproductive dysfunction induced by the abusive drug, such as cocaine. In the present study, we investigated whether NFκB mediates cocaine-induced reproductive dysfunction in m...
Source: Chemico Biological Interactions - May 4, 2019 Category: Biochemistry Source Type: research

Polyethers isolated from the marine actinobacterium Streptomyces cacaoi inhibit autophagy and induce apoptosis in cancer cells
In this study, three polyether-type metabolites (1–3), one of which was a new natural product (3), were isolated from the marine derived Streptomyces cacaoi via antimicrobial activity-guided fractionation studies. As several polyether compounds with structural similarity such as monensin have been linked with autophagy and cell death, we first assessed the cytotoxicity of these three compounds. Compounds 2 and 3, but not 1, were found to be cytotoxic in several cell lines with a higher potency towards cancer cells. Furthermore, 2 and 3 caused accumulation of both autophagy flux markers LC3-II and p62 along with cleav...
Source: Chemico Biological Interactions - May 4, 2019 Category: Biochemistry Source Type: research

Protection of the myocardium against ischemia/reperfusion injury by punicalagin through an SIRT1-NRF-2-HO-1-dependent mechanism
Publication date: Available online 4 May 2019Source: Chemico-Biological InteractionsAuthor(s): Li-Ming Yu, Xue Dong, Xiao-Dong Xue, Jian Zhang, Zhi Li, Hong-Jiang Wu, Zhong-Lu Yang, Yang Yang, Hui-Shan WangAbstractPunicalagin has been found to exert cardiac protective effects against myocardial ischemia/reperfusion (MI/R) injury, although the detailed mechanisms remain largely unknown. This experiment was performed to explore the potential involvement of silent information regulator 1 (SIRT1)-NFE2-related factor 2 (NRF-2)-heme oxygenase-1 (HO-1) pathway in the cardiac protective actions of punicalagin. Sprague-Dawley (SD) ...
Source: Chemico Biological Interactions - May 4, 2019 Category: Biochemistry Source Type: research

Glaucocalyxin A reverses EMT and TGF-β1-induced EMT by inhibiting TGF-β1/Smad2/3 signaling pathway in osteosarcoma
In this study, we investigated the potential mechanisms of Glaucocalyxin A on EMT and metastasis of osteosarcoma. We found that Glaucocalyxin A inhibited migration and invasion of MG-63 and 143B cells. Moreover, Glaucocalyxin A increased the protein and mRNA levels of E-cadherin and decreased the protein and transcription expression of N-cadherin, Vimentin. Glaucocalyxin A also inhibited the protein and mRNA levels of EMT-associated transcription factor including Snail and Slug. Furthermore, Glaucocalyxin A inhibited transforming growth factor-β1 (TGF-β1)-induced migration, invasion and EMT of low-metastatic oste...
Source: Chemico Biological Interactions - May 4, 2019 Category: Biochemistry Source Type: research

Neutral endopeptidase (NEP) inhibitors – Thiorphan, sialorphin, and its derivatives exert anti-proliferative activity towards colorectal cancer cells in vitro
In this study, the anti-cancer effects of NEP inhibitors, thiorphan (synthetic compound) and sialorphin (naturally occurring pentapeptide) on CRC cells were investigated. Moreover, we synthesized some derivatives of sialorphin (alanine scan analogues: AHNPR, QANPR, QHAPR, QHNAR; N-acetylated sialorphin; C-amidated sialorphin, and C-amidated alanine scan analogues) to examine the biological activity of these inhibitors on CRC cells. The cytotoxic activity of the NEP inhibitors against CRC cell lines (SW620 and LS180) and normal human fibroblasts (HSF) was evaluated. Additionally, the influence of NEP inhibitors on prolifera...
Source: Chemico Biological Interactions - May 2, 2019 Category: Biochemistry Source Type: research

Toxicity of carbon-based nanomaterials: Reviewing recent reports in medical and biological systems
Publication date: Available online 1 May 2019Source: Chemico-Biological InteractionsAuthor(s): Rasoul Madannejad, Nahid Shoaie, Fatemeh Jahanpeyma, Mohammad Hasan Darvishi, Mostafa Azimzadeh, Hamidreza JavadiAbstractApplication of nanomaterials in our daily life is increasing, day in day out and concerns have raised about their toxicity for human and other organisms. In this manner, carbon-based nanomaterials have been applied to different products due to their unique physicochemical, electrical, mechanical properties, and biological compatibility. But, there are several reports about the negative effects of these material...
Source: Chemico Biological Interactions - May 1, 2019 Category: Biochemistry Source Type: research

Azodyes as markers for tumor hypoxia imaging and therapy: An up-to-date review
Publication date: Available online 30 April 2019Source: Chemico-Biological InteractionsAuthor(s): Rashmi Kumari, Dhanya Sunil, Raghumani S. Ningthoujam, NV Anil KumarAbstractTumor hypoxia is the low tissue oxygen levels seen characteristically in rapidly proliferating and expanding neoplasms. It affects both malignant tumor cells and its microenvironment, resulting in dysfunctional neovascularization. This leads to epithelial-to-mesenchymal transition phenotype, facilitating tumor progression through cell mobility, invasion, and metastasis. The hypoxic condition in solid tumors is thus an indicator of the process of cancer...
Source: Chemico Biological Interactions - May 1, 2019 Category: Biochemistry Source Type: research

(−)-Epigallocatechin-3-gallate attenuates the toxicity of methylmercury in Caenorhabditis elegans by activating SKN-1
Publication date: Available online 30 April 2019Source: Chemico-Biological InteractionsAuthor(s): Mo Chen, Fan Wang, Jing-Jing Cao, Xue Han, Wei-Wei Lu, Xin Ji, Wei-Hong Chen, Wen-Qing Lu, Ai-Lin LiuAbstract(−)-Epigallocatechin-3-gallate (EGCG) found in tea is a natural activator of nuclear factor erythroid 2-related factor 2 (Nrf2), a primary regulator of the cellular defense system. The adverse health effects resulting from methylmercury (MeHg) exposure in humans are of worldwide concern. We hypothesized that EGCG could induce a Nrf2-mediated protective response to antagonize MeHg toxicity. Using the Caenorhabditis...
Source: Chemico Biological Interactions - May 1, 2019 Category: Biochemistry Source Type: research

Honokiol post-treatment ameliorates myocardial ischemia/reperfusion injury by enhancing autophagic flux and reducing intracellular ROS production
Publication date: Available online 29 April 2019Source: Chemico-Biological InteractionsAuthor(s): Zhipeng Tan, Haiqiong Liu, Xudong Song, Yuanna Ling, Shangfei He, Yifeng Yan, Jing Yan, Siyi Wang, Xianbao Wang, Aihua ChenAbstractHonokiol (HKL) is a natural low-molecular-weight biphenolic compound derived from the bark of magnolia trees. Previous studies indicate that HKL exerts potent cardioprotective effects on ischemia/reperfusion (I/R) injury; however, evidence of the further relationship between HKL posttreatment and myocardial I/R injury has not been clearly found. In our study, we explored the protective effect of HK...
Source: Chemico Biological Interactions - April 29, 2019 Category: Biochemistry Source Type: research

Activation of G protein-coupled receptor 30 by flavonoids leads to expression of acetylcholinesterase in cultured PC12 cells
Publication date: Available online 26 April 2019Source: Chemico-Biological InteractionsAuthor(s): Etta Y.L. Liu, Miranda L. Xu, Yingjie Xia, Xiangpeng Kong, Qiyun Wu, Tina T.X. Dong, Karl W.K. TsimAbstractFlavonoids, considered as phytoestrogen mainly deriving from fruit and vegetable, are known to have beneficial effects in brain functions. The role of flavonoids in induction of a cholinergic enzyme, acetylcholinesterase (AChE), was being explored here. In cultured PC12 cells, twenty-four commonly found flavonoids were tested for its induction on AChE activity. Fourteen flavonoids showed induction, and five of them had ...
Source: Chemico Biological Interactions - April 28, 2019 Category: Biochemistry Source Type: research

Eudesmin exerts antitumor effects by down-regulating EZH2 expression in nasopharyngeal carcinoma cells
In conclusion, eudesmin exhibited antitumor activity via downregulating EZH2 expression through the inhibition of Akt signaling pathway. Eudesmin could be developed as a new pharmacologic approach for NPC treatment. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - April 25, 2019 Category: Biochemistry Source Type: research

Engineering aldo-keto reductase 1B10 to mimic the distinct 1B15 topology and specificity towards inhibitors and substrates, including retinoids and steroids
Publication date: Available online 24 April 2019Source: Chemico-Biological InteractionsAuthor(s): Joan Giménez-Dejoz, Susanne Weber, Álvaro Fernández-Pardo, Gabriele Möller, Jerzy Adamski, Sergio Porté, Xavier Parés, Jaume FarrésAbstractThe aldo-keto reductase (AKR) superfamily comprises NAD(P)H-dependent enzymes that catalyze the reduction of a variety of carbonyl compounds. AKRs are classified in families and subfamilies. Humans exhibit three members of the AKR1B subfamily: AKR1B1 (aldose reductase, participates in diabetes complications), AKR1B10 (overexpressed in several c...
Source: Chemico Biological Interactions - April 25, 2019 Category: Biochemistry Source Type: research

Aldose reductase inhibition enhances lens regeneration in mice
Publication date: Available online 23 April 2019Source: Chemico-Biological InteractionsAuthor(s): Leonid M. Zukin, Michelle G. Pedler, Kevin Chyung, Sarah Seiwald, Patricia Lenhart, Biehuoy Shieh, J. Mark PetrashAbstractAfter cataract surgery, epithelial cells lining the anterior lens capsule can transition to one of two divergent pathways, including fibrosis which leads to posterior capsular opacification (PCO), or lens fiber cell differentiation which leads to regeneration of lens material. We previously showed that the PCO response can be suppressed with aldose reductase (AR) inhibitors. In this present study we show th...
Source: Chemico Biological Interactions - April 23, 2019 Category: Biochemistry Source Type: research

Downregulation of microRNA-200c-3p reduces damage of hippocampal neurons in epileptic rats by upregulating expression of RECK and inactivating the AKT signaling pathway
ConclusionOur study provides evidence that downregulation of miR-200c-3p reduces damage of hippocampal neurons in epileptic rats by upregulating RECK and inactivating the AKT signaling pathway.Graphical abstract (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - April 22, 2019 Category: Biochemistry Source Type: research

Inhibitory effect of mabuterol on proliferation of rat ASMCs induced by PDGF-BB via regulating [Ca2+]i and mitochondrial fission/fusion
This study is aimed to investigate whether Mabuterol (Mab) inhibits proliferation of airway smooth muscle cells (ASMCs) induced by platelet-derived growth factor BB (PDGF-BB) and how far it is related to mitochondrial fission/fusion and intracellular calcium if it comes into play. To explore the mechanism of Mab's antagonizing the proliferation, Mdivi-1, DRP1 inhibitor, which has an inhibitory effect on mitochondrial fission, is used to compare with Mab. Cell viability was measured by either MTT or CCK-8. The inhibitory effect of Mab on S phase of ASM cell cycle induced by PDGF-BB was analyzed by flow cytometry (FCM). Fluo...
Source: Chemico Biological Interactions - April 20, 2019 Category: Biochemistry Source Type: research

Computer-designed active human butyrylcholinesterase double mutant with a new catalytic triad
Publication date: Available online 19 April 2019Source: Chemico-Biological InteractionsAuthor(s): Bella L. Grigorenko, Dana A. Novichkova, Sofya V. Lushchekina, Irina V. Zueva, Lawrence M. Schopfer, Alexander V. Nemukhin, Sergey D. Varfolomeev, Oksana Lockridge, Patrick MassonAbstractA computer-designed mutant of human butyrylcholinesterase (BChE), N322E/E325G, with a novel catalytic triad was made. The catalytic triad of the wild-type enzyme (S198·H438·E325) was replaced by S198·H438·N322E in silico. Molecular dynamics for 1.5 μs and Markov state model analysis showed that the new catalytic ...
Source: Chemico Biological Interactions - April 20, 2019 Category: Biochemistry Source Type: research

Plantamajoside attenuates isoproterenol-induced cardiac hypertrophy associated with the HDAC2 and AKT/ GSK-3β signaling pathway
Publication date: Available online 19 April 2019Source: Chemico-Biological InteractionsAuthor(s): Linlin Shang, Lv Pin, Shanshan Zhu, Xiaohang Zhong, Yubiao Zhang, Mao Shun, Yunen Liu, Mingxiao HouAbstractAs a compensatory response to cardiac overload, cardiac hypertrophy is closely associated with the occurrence and development of a variety of cardiovascular diseases, in which histone deacetylase 2 (HDAC2) has been reported to play an important role. Plantamajoside (PMS) is an active component extracted from Herba Plantaginis, which is a traditional Chinese medicine, and many biological activities of PMS have been reporte...
Source: Chemico Biological Interactions - April 20, 2019 Category: Biochemistry Source Type: research

Ameliorative effect of zinc supplementation on compromised small intestinal health in streptozotocin-induced diabetic rats
Publication date: Available online 19 April 2019Source: Chemico-Biological InteractionsAuthor(s): Susmita Barman, Krishnapura SrinivasanAbstractZinc depletion during diabetes postulates a role for zinc nutrition in the management of associated complications. The present study evaluated zinc supplementation for countering the compromised intestinal integrity through moderation of oxidative stress and suppression of stress-stimulated inflammatory proliferation in streptozotocin-induced diabetic rats. Diabetic rats were provided with supplemental zinc for six weeks (5 and 10-times of normal level). Supplemental zinc nurtured ...
Source: Chemico Biological Interactions - April 20, 2019 Category: Biochemistry Source Type: research

Long non-coding RNA-p21 regulates MPP+-induced neuronal injury by targeting miR-625 and derepressing TRPM2 in SH-SY5Y cells
Publication date: Available online 18 April 2019Source: Chemico-Biological InteractionsAuthor(s): Xiu-Ming Ding, Lian-Jiang Zhao, Han-Yong Qiao, Shao-Lan Wu, Xi-Hui WangAbstractParkinson's disease (PD), the second most prevalent age-related neurodegenerative disease, occurs as a result of the loss of dopaminergic neurons in the substantia nigra. Long non-coding RNA-p21 (lnc-p21) has been demonstrated to be upregulated in PD. However, its role in PD is unknown. Here, the results showed that lnc-p21 was highly expressed in human neuroblastoma SH-SY5Y cells treated with MPP+. Knockdown of lnc-p21 attenuated the cytotoxicity a...
Source: Chemico Biological Interactions - April 19, 2019 Category: Biochemistry Source Type: research