Lipopolysaccharide modification enhances the inhibitory effect of clodronate liposomes on hepatic fibrosis by depletion of macrophages and hepatic stellate cells
This study aimed to evaluate the therapeutic efficacy of lipopolysaccharide-coupled clodronate liposomes (LPS-CLD-lipos) and the effects of liposomes size on hepatic fibrosis. Three rat models of hepatic fibrosis were established in vivo; diethylnitrosamine (DEN), bile duct ligation (BDL), and carbon tetrachloride (CCl4). Hematoxylin and eosin staining and serological liver function indices were used to analyze pathological liver damage. Masson's trichrome and Sirius red staining were used to evaluate the effect of liposomes on liver collagen fibers. The hydroxyproline content in liver tissues was determined. In vitro cell...
Source: Chemico-Biological Interactions - April 25, 2024 Category: Molecular Biology Authors: Luyao Zhang Min Tao Hengyan Zhang Shichao Zhang Xiaojuan Hou Chen Zong Gangqi Sun Shiyao Feng Haixin Yan Ying Lu Xue Yang Lixin Wei Li Zhang Source Type: research
Nitazoxanide protects against experimental ulcerative colitis through improving intestinal barrier and inhibiting inflammation
In conclusion, nitazoxanide protects against DSS-induced ulcerative colitis in mice through improving intestinal barrier and inhibiting inflammation and the underlying mechanism involves AMPK activation and JAK2/STAT3 inhibition.PMID:38663798 | DOI:10.1016/j.cbi.2024.111013 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - April 25, 2024 Category: Molecular Biology Authors: Hu-Tai-Long Zhu Jing Luo Yi Peng Xiao-Fan Cheng Shang-Ze Wu Yin-Di Zhao Le Chang Zhi-Jie Sun De-Li Dong Source Type: research
Single and mixture effects of bisphenol A and benzophenone-3 on in vitro T helper cell differentiation
In conclusion, our study provides solid evidence for both, the immune disruptive potential and the existence of cumulative effects of real nature EDC mixtures on T cell in vitro differentiation.PMID:38653352 | DOI:10.1016/j.cbi.2024.111011 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - April 23, 2024 Category: Molecular Biology Authors: Florence Fischer Miriam Rebecca Ermer Julia Howanski Ziran Yin Mario Bauer Marita Wagner Beate Fink Ana C Zenclussen Anne Schumacher Source Type: research
Single and mixture effects of bisphenol A and benzophenone-3 on in vitro T helper cell differentiation
In conclusion, our study provides solid evidence for both, the immune disruptive potential and the existence of cumulative effects of real nature EDC mixtures on T cell in vitro differentiation.PMID:38653352 | DOI:10.1016/j.cbi.2024.111011 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - April 23, 2024 Category: Molecular Biology Authors: Florence Fischer Miriam Rebecca Ermer Julia Howanski Ziran Yin Mario Bauer Marita Wagner Beate Fink Ana C Zenclussen Anne Schumacher Source Type: research
Interaction of Exogenous Acetylcholinesterase and Butyrylcholinesterase with Amyloid- β Plaques in Human Brain Tissue
Chem Biol Interact. 2024 Apr 20:111012. doi: 10.1016/j.cbi.2024.111012. Online ahead of print.ABSTRACTAcetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are associated with amyloid-β (Aβ) plaques and exhibit altered biochemical properties in human Alzheimer's disease (AD), as well as in the transgenic 5XFAD mouse model of AD amyloidosis. In the brains of the 5XFAD mouse model devoid of BChE enzyme (5XFAD/BChE-KO), incubation of tissue sections with exogenous BChE purified from human plasma (pl-BChE) leads to its association with Aβ plaques and its biochemical properties are comparable to those reported for end...
Source: Chemico-Biological Interactions - April 22, 2024 Category: Molecular Biology Authors: G A Reid S Darvesh Source Type: research
Biochanin A ameliorates DSS-induced ulcerative colitis by improving colonic barrier function and protects against the development of spontaneous colitis in the Muc2 deficient mice
In this study, we used a dextran sodium sulfate (DSS)-induced ulcerative colitis model and the Muc2 deficient (Muc2-/-) mice spontaneous colitis model to explore the mechanisms of BCA in the treatment of UC. Here, we verified that DSS-induced UC was observably attenuated and spontaneous colitis in Muc2-/- mice was relieved by BCA. Treatment with BCA improved colitis-related symptoms and reduced intestinal permeability by upregulating the levels of goblet cells and tight junction (TJ) proteins. In addition, we confirmed that BCA promotes autophagy through the AMP-activated protein kinase (AMPK)/mammalian target of rapamycin...
Source: Chemico-Biological Interactions - April 22, 2024 Category: Molecular Biology Authors: Haina Zhang Xueqi Wang Linxian Zhao Kai Zhang Jiaming Cui Guangmeng Xu Source Type: research
Mitomycin C and Its Analog Trigger Cytotoxicity in MCF-7 and K562 cancer cells through the regulation of RAS and MAPK/ERK pathways
Chem Biol Interact. 2024 Apr 18:111007. doi: 10.1016/j.cbi.2024.111007. Online ahead of print.ABSTRACTMitomycin C (MC) is an anti-cancer drug which functions by forming interstrand crosslinks (ICLs) between opposing DNA strands. MC analog, 10-decarbamoyl mitomycin C (DMC), unlike MC, has stronger cytotoxic effects on cancer cells with TP53 mutation. We previously demonstrated that MC/DMC could activate p21WAF1/CIP1 in MCF-7 (TP53-proficient) and K562 (TP53 deficient) cells in a TP53-independent mode. We also found that MC/DMC regulate AKT activation in a TP53-dependent manner and that AKT deactivation is not associated wit...
Source: Chemico-Biological Interactions - April 20, 2024 Category: Molecular Biology Authors: Owen Zacarias Cristina C Clement Shu-Yuan Cheng Melissa Rosas Christina Gonzalez Marion Peter Peter Coopman Elise Champeil Source Type: research
Revolutionising Lung Health: Exploring the Latest Breakthroughs and Future Prospects of Synbiotic Nanostructures in Lung Diseases
Chem Biol Interact. 2024 Apr 17:111009. doi: 10.1016/j.cbi.2024.111009. Online ahead of print.ABSTRACTThe escalating prevalence of lung diseases underscores the need for innovative therapies. Dysbiosis in human body microbiome has emerged as a significant factor in these diseases, indicating a potential role for synbiotics in restoring microbial equilibrium. However, effective delivery of synbiotics to the target site remains challenging. Here, we aim to explore suitable nanoparticles for encapsulating synbiotics tailored for applications in lung diseases. Nanoencapsulation has emerged as a prominent strategy to address th...
Source: Chemico-Biological Interactions - April 19, 2024 Category: Molecular Biology Authors: Ayeh Bani Saeid Gabriele De Rubis Kylie A Williams Stewart Yeung Dinesh Kumar Chellappan Sachin Kumar Singh Gaurav Gupta Philip M Hansbro Mohammad-Ali Shahbazi Monica Gulati Indu Pal Kaur H élder A Santos Keshav Raj Paudel Kamal Dua Source Type: research
Effective skin decontamination with RSDL ® (Reactive Skin Decontamination Lotion Kit) following dermal exposure to a Novichok class nerve agent
This study determines RSDL decontamination as an effective treatment strategy for dermal exposure to the Novichok agent A234 and underscores the importance of early, forward use of skin decontamination, as rapidly as possible.PMID:38641146 | DOI:10.1016/j.cbi.2024.111001 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - April 19, 2024 Category: Molecular Biology Authors: Alex S Cornelissen Roland M van den Berg Jan P Langenberg Marco van Grol Rowdy Bross John Pittman Laura Cochrane Vladimir Savransky Source Type: research
Therapeutic targeting of thioredoxin reductase 1 causes ferroptosis while potentiating anti-PD-1 efficacy in head and neck cancer
This study is the first to highlight TXNRD1 as a crucial factor contributing to resistance to anti-PD-1 treatment in HNSCC. In this study, we identified targetable regulators of resistance to immunotherapy-induced ferroptosis in HNSCC. We observed a link of thioredoxin reductase 1 (TXNRD1) with tumoral PD-L1 expression and ferroptosis suppression in HNSCC. Moreover, HNSCC tumors with aberrant TXNRD1 expression exhibited a lack of PD-1 response, NRF2 overexpression, and PD-L1 upregulation. TXNRD1 inhibition promoted ferroptosis in HNSCC cells with NRF2 activation and in organoid tumors derived from patients lacking a PD-1 r...
Source: Chemico-Biological Interactions - April 18, 2024 Category: Molecular Biology Authors: Ming-Shou Hsieh Hang Huong Ling Syahru Agung Setiawan Mardiah Suci Hardianti Iat-Hang Fong Chi-Tai Yeh Jia-Hong Chen Source Type: research
Role of reactive carbonyls and superoxide radicals in protein damage by cigarette smoke extracts: Comparison of Heat-not-Burn e-cigarettes to conventional cigarettes
This study investigated the relative importance of these pathways during exposure of a model protein to fresh cigarette emission extracts. Briefly, protein carbonyl adducts were estimated in bovine serum albumin following incubation in buffered solutions with whole cigarette emissions extracts prepared from either a single 1R6F research cigarette or a single "Heat-not-Burn" e-cigarette. Although both extracts caused concentration-dependent protein carbonylation, conventional cigarette extracts produced higher adduct yields than e-cigarette extracts. Superoxide radical generation by conventional and e-cigarette emissions wa...
Source: Chemico-Biological Interactions - April 18, 2024 Category: Molecular Biology Authors: Nicholas Merritt Cameron Urquhart Philip Burcham Source Type: research
In vitro cleavage of tumor necrosis factor α (TNFα) by Signal-Peptide-Peptidase-like 2b (SPPL2b) resembles mechanistic principles observed in the cellular context
We describe the details of developing an in vitro cleavage assay for SPPL2b and its model substrate TNFα and analyzed the influence of phospholipids, detergent supplements, and cholesterol on the SPPL2b in vitro activity. SPPL2b in vitro activity resembles mechanistic principles that have been observed in a cellular context, such as cleavage sites and consecutive turnover of the TNFα transmembrane domain. The novel in vitro cleavage assay is functional with separately isolated protease and substrate and amenable to a high throughput plate-based readout overcoming previous limitations and providing the basis for studying ...
Source: Chemico-Biological Interactions - April 18, 2024 Category: Molecular Biology Authors: Kinda Sharrouf Christine Schlosser Sandra Mildenberger Regina Fluhrer Sabine Hoeppner Source Type: research
Therapeutic Targeting of Thioredoxin Reductase 1 Causes Ferroptosis while Potentiating Anti-PD-1 Efficacy in Head and Neck Cancer
This study is the first to highlight TXNRD1 as a crucial factor contributing to resistance to anti-PD-1 treatment in HNSCC. In this study, we identified targetable regulators of resistance to immunotherapy-induced ferroptosis in HNSCC. We observed a link of thioredoxin reductase 1 (TXNRD1) with tumoral PD-L1 expression and ferroptosis suppression in HNSCC. Moreover, HNSCC tumors with aberrant TXNRD1 expression exhibited a lack of PD-1 response, NRF2 overexpression, and PD-L1 upregulation. TXNRD1 inhibition promoted ferroptosis in HNSCC cells with NRF2 activation and in organoid tumors derived from patients lacking a PD-1 r...
Source: Chemico-Biological Interactions - April 18, 2024 Category: Molecular Biology Authors: Ming-Shou Hsieh Hang Huong Ling Syahru Agung Setiawan Mardiah Suci Hardianti Iat-Hang Fong Chi-Tai Yeh Jia-Hong Chen Source Type: research
Role of reactive carbonyls and superoxide radicals in protein damage by cigarette smoke extracts: Comparison of Heat-Not-Burn e-cigarettes to conventional cigarettes
This study investigated the relative importance of these pathways during exposure of a model protein to fresh cigarette emission extracts. Briefly, protein carbonyl adducts were estimated in bovine serum albumin following incubation in buffered solutions with whole cigarette emissions extracts prepared from either a single 1R6F research cigarette or a single "Heat-not-Burn" e-cigarette. Although both extracts caused concentration-dependent protein carbonylation, conventional cigarette extracts produced higher adduct yields than e-cigarette extracts. Superoxide radical generation by conventional and e-cigarette emissions wa...
Source: Chemico-Biological Interactions - April 18, 2024 Category: Molecular Biology Authors: Nicholas Merritt Cameron Urquhart Philip Burcham Source Type: research
In vitro cleavage of tumor necrosis factor α (TNFα) by Signal-Peptide-Peptidase-like 2b (SPPL2b) resembles mechanistic principles observed in the cellular context
We describe the details of developing an in vitro cleavage assay for SPPL2b and its model substrate TNFα and analyzed the influence of phospholipids, detergent supplements, and cholesterol on the SPPL2b in vitro activity. SPPL2b in vitro activity resembles mechanistic principles that have been observed in a cellular context, such as cleavage sites and consecutive turnover of the TNFα transmembrane domain. The novel in vitro cleavage assay is functional with separately isolated protease and substrate and amenable to a high throughput plate-based readout overcoming previous limitations and providing the basis for studying ...
Source: Chemico-Biological Interactions - April 18, 2024 Category: Molecular Biology Authors: Kinda Sharrouf Christine Schlosser Sandra Mildenberger Regina Fluhrer Sabine Hoeppner Source Type: research
