Catalytic bioscavengers as countermeasures against organophosphate nerve agents.
Abstract Recent years have seen an increasing number of incidence, in which organophosphate nerve agents (OPNAs) have been used against civilians with devastating outcomes. Current medical countermeasures against OPNA intoxications are aimed at mitigating their symptoms, but are unable to effectively prevent them. In addition, they may fail to prevent the onset of a cholinergic crisis in the brain and its secondary toxic manifestations. The need for improved medical countermeasures has led to the development of bioscavengers; proteins and enzymes that may prevent intoxication by binding and inactivating OPNAs befo...
Source: Chemico-Biological Interactions - July 7, 2018 Category: Molecular Biology Authors: Goldsmith M, Ashani Y Tags: Chem Biol Interact Source Type: research

New chalcone derivative exhibits antiproliferative potential by inducing G2/M cell cycle arrest, mitochondrial-mediated apoptosis and modulation of MAPK signalling pathway.
In conclusion, our data showed ability of 1C to suppress cancel cell growth and provide the rationale for further in vivo study. PMID: 29981726 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - July 5, 2018 Category: Molecular Biology Authors: Takac P, Kello M, Pilatova MB, Kudlickova Z, Vilkova M, Slepcikova P, Petik P, Mojzis J Tags: Chem Biol Interact Source Type: research

Thymoquinone induces apoptosis in bladder cancer cell via endoplasmic reticulum stress-dependent mitochondrial pathway.
Abstract BACKGROUND: Thymoquinone (TQ), the major active compound isolated from black seed oil (Nigella sativa), has been reported to exhibit anti-inflammatory and anticancer abilities. However, the exact molecular mechanism underlying the anticancer effect of TQ is still poorly understood, especially in regard to TQ's effect on endoplasmic reticulum stress-mediated apoptosis. METHODS: The cytotoxicity of TQ on T24 and 253J bladder cancer cells was analyzed by MTT assay, colony formation assay and Annexin V-FITC/PI staining, while SV-HUC-1, the SV-40 immortalized human uroepithelial cell line, was used as a n...
Source: Chemico-Biological Interactions - July 2, 2018 Category: Molecular Biology Authors: Zhang M, Du H, Huang Z, Zhang P, Yue Y, Wang W, Liu W, Zeng J, Ma J, Chen G, Wang X, Fan J Tags: Chem Biol Interact Source Type: research

Level of NO/nitrite and 3-nitrotyrosine in seminal plasma of infertile men: Correlation with sperm number, motility and morphology.
Abstract Nitric oxide (NO) is a signaling molecule responsible for initiation of molecular events that influence sperm functionality in a concentration-dependent manner. It is still not fully understood how seminal plasma NO contributes to sperm pathologies. The aim of this study was to elucidate whether and how NO is implicated in etiology of different sperm abnormalities. To this end we determine NO, nitrite and 3-nitrotyrosine (3-NT) content in seminal plasma of infertile men with specific pathologies (terato-, oligoterato- and oligoasthenoteratospermia) and relate it to infertile normospermic samples. To gain ...
Source: Chemico-Biological Interactions - July 2, 2018 Category: Molecular Biology Authors: Kalezic A, Macanovic B, Garalejic E, Korac A, Otasevic V, Korac B Tags: Chem Biol Interact Source Type: research

Honey protects against wings posture error and molecular changes related to mitochondrial pathways induced by hypoxia/reoxygenation in adult Drosophila melanogaster.
In conclusion, we report for the first time the protective effects of honey against complex phenotypes and mitochondrial changes induced by H/R in adult flies. PMID: 29964003 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 28, 2018 Category: Molecular Biology Authors: Cruz LC, Ecker A, Rodrigues NR, Martins IK, Posser T, Maciel FE, Vargas MA, Barbosa NV, Franco JL Tags: Chem Biol Interact Source Type: research

Effects of silver sulfide quantum dots coated with 2-mercaptopropionic acid on Genotoxic and apoptotic pathways in vitro.
In this study, we aim to determine the cytotoxicity of 2 MP A/Ag2S in Chinese hamster lung fibroblast (V79) cells. The genotoxic and apoptotic effects of 2 MP A/Ag2S QDs were assessed by the alkaline single cell electrophoresis assay and real time polymerase chain reaction techniques, respectively. The cell viability decreased above 200 μg/ml and 800 μg/ml for MTT tetrazolium and neural red uptake assays, respectively. DNA damage was not observed by 2 MP A/Ag2S QDs at the studied concentration levels (5-2000 μg/ml). The levels of mRNA expression of p53, caspase 3, caspase 9, bax, bcl-2, survivin ...
Source: Chemico-Biological Interactions - June 26, 2018 Category: Molecular Biology Authors: Vardar DO, Aydin S, Hocaoglu I, Yagci Acar FH, Basaran N Tags: Chem Biol Interact Source Type: research

Impact of intrauterine exposure to betamethasone on the testes and epididymides of prepubertal rats.
Abstract Therapy with betamethasone, a synthetic glucocorticoid, is used in cases of preterm birth risk, in order to promote fetal lung maturation, and decrease neonatal mortality and morbidity. However, late reproductive disorders related to the prenatal exposure to this compound have been reported by our Laboratory, in both male and female rats. Thus, the present study aimed to evaluate the impact of betamethasone on postnatal reproductive development, during pre-puberty, of male offspring exposed in utero to this synthetic glucocorticoid. For this purpose, pregnant Wistar rats were allocated into two groups: Co...
Source: Chemico-Biological Interactions - June 25, 2018 Category: Molecular Biology Authors: de Barros JWF, Borges CDS, Missassi G, Pacheco TL, De Grava Kempinas W Tags: Chem Biol Interact Source Type: research

Mechanistic-based non-animal assessment of eye toxicity: Inflammatory profile of human keratinocytes cells after exposure to eye damage/irritant agents.
In this study, the Cytometric Bead Array (CBA) and ELISA assays were used to evaluate the inflammatory cytokine profile produced by HaCaT human keratinocytes after exposure to chemicals with different UN GHS eye toxicity classifications, aiming to stablish a correlation between inflammatory endpoints and eye toxicity (damage/irritation) potential. As a first step, cytotoxic profile of the chemicals, including 3 non-irritants and 10 eye toxicants (GHS Category 1, 2A and 2B), was evaluated after 24 h exposure using MTT assay and Inhibitory Concentration of 20% of cell viability (IC20) was calculated for each chemical. Then...
Source: Chemico-Biological Interactions - June 25, 2018 Category: Molecular Biology Authors: da Silva ACG, Chialchia AR, de Ávila RI, Valadares MC Tags: Chem Biol Interact Source Type: research

Hesperidin protects against stress induced gastric ulcer through regulation of peroxisome proliferator activator receptor gamma in diabetic rats.
Abstract Stress induced gastric ulcer is a serious health problem in diabetic patients. Some studies reported that hesperidin (HDN), a citrus bioflavonoid, can bind to and stimulate peroxisome proliferator-activator receptor-gamma (PPAR-γ) which may mediate its antidiabetic, anti-inflammatory and anti-oxidant effects. This work aims to study the possible protective effect of HDN against stress induced gastric ulcer in diabetic rats as well as the possible involvement of PPARγ in this effect. Type 2 diabetes was induced using streptozotocin and nicotinamide. Diabetic rats received either HDN (100 m...
Source: Chemico-Biological Interactions - June 23, 2018 Category: Molecular Biology Authors: Elshazly SM, Abd El Motteleb DM, Ibrahim IAAE Tags: Chem Biol Interact Source Type: research

Correlation of structural features of novel 1,2,3-triazoles with their neurotoxic and tumoricidal properties.
Silva AD Abstract Triazoles are interesting templates for novel chemotherapeutic drugs. We synthesized here 17 1,3,4-substituted-1,2,3-triazoles that differed in their 1'-substituent (variable alkyl chain lengths C3-C12), the 3'-substituent (no substituent, -methyl or -propyl) or the salt form obtained. Several of the compounds were cytotoxic (μM range) for tumor cells (HL-60, JURKAT, MCF-7, HCT-116), and when the effect was compared to non-transformed cells (Vero), selectivity ratios of up to 23-fold were obtained. To estimate the liability of these potential drug candidates for triggering neurotoxicity, we u...
Source: Chemico-Biological Interactions - June 23, 2018 Category: Molecular Biology Authors: de Souza-Fagundes EM, Delp J, Prazeres PDM, Marques LB, Carmo AML, Stroppa PHF, Glanzmann N, Kisitu J, Szamosvàri D, Böttcher T, Leist M, da Silva AD Tags: Chem Biol Interact Source Type: research

Methyl ferulic acid attenuates ethanol-induced hepatic steatosis by regulating AMPK and FoxO1 Pathways in Rats and L-02 cells.
We examined the effect of MFA on ethanol-induced lipid deposition in L-02 cells and SD rats. The results showed that MFA reduced the accumulation of lipid in L-02 cells, improved alcoholic liver injury in rats, alleviated hepatic pathological lesions, and reduced lipid deposition in rat serum and liver. Further studies suggest that MFA reduces lipid synthesis by activating AMPK-ACC/MAPK-FoxO1 pathway. In addition, MFA also promotes lipid oxidation by up-regulating the expression of SIRT1, PPAR-α, and CPT-1α. Taken together, MFA ameliorates ethanol-induced hepatic steatosis by activating AMPK-ACC/MAPK-FoxO1 ...
Source: Chemico-Biological Interactions - June 22, 2018 Category: Molecular Biology Authors: Cheng Q, Li YW, Yang CF, Zhong YJ, He H, Zhu FC, Li L Tags: Chem Biol Interact Source Type: research

Molecular mechanism of composite nanoparticles TiO2/WO3/GO-induced activity changes of catalase and superoxide dismutase.
Abstract More and more composite nano-photocatalysts were developed by doping, modifying and coupling, which expanded its application but resulted in pollution due to the unrecyclability. Composite photocatalyst TiO2/WO3/GO, as a model, was evaluated by exploring the molecular mechanism of TiO2/WO3/GO-induced activity changes of catalase (CAT) and superoxide dismutase (SOD). Results showed that TiO2/WO3/GO could lead to conformational and functional changes of CAT and SOD. The activity of both CAT and SOD increased depending on the exposure dose of TiO2/WO3/GO. The change skeleton structure and increase of α...
Source: Chemico-Biological Interactions - June 21, 2018 Category: Molecular Biology Authors: Hao X, Zhang L, Zheng X, Zong W, Liu C Tags: Chem Biol Interact Source Type: research

A test battery for assessing the ecotoxic effects of textile dyes.
Oliveira DP Abstract The textile dyeing industry is one of the main sectors contributing to environmental pollution, due to the generation of large amounts of wastewater loaded with dyes (ca. 2-50% of the initial amount of dyes used in the dye baths is lost), causing severe impacts on human health and the environment. In this context, an ecotoxicity testing battery was used to assess the acute toxicity and genotoxicity of the textile dyes Direct Black 38 (DB38; azo dye) and Reactive Blue 15 (RB15; copper phthalocyanine dye) on different trophic levels. Thus these dyes were tested using the following assays: Filter...
Source: Chemico-Biological Interactions - June 21, 2018 Category: Molecular Biology Authors: de Oliveira GAR, Leme DM, de Lapuente J, Brito LB, Porredón C, de Brito Rodrigues L, Brull N, Serret JT, Borràs M, Disner GR, Cestari MM, de Oliveira DP Tags: Chem Biol Interact Source Type: research

Monomeric and oligomeric flavanols maintain the endogenous glucocorticoid response in human macrophages in pro-oxidant conditions in vitro.
er AR Abstract Chronic inflammation and oxidative stress are (sub)cellular processes that enhance each other and contribute to the genesis of many systemic pathologies. The endogenous glucocorticoid cortisol plays an important role in the physiological termination of a pro-inflammatory immune response. However, in conditions of pronounced oxidative stress the anti-inflammatory action of cortisol is impaired. Since grape seed-derived monomeric and oligomeric flavan-3-ols (MOF) have been shown to attenuate both, inflammation and oxidative stress in vitro and in humans, we hypothesized that these compounds are able t...
Source: Chemico-Biological Interactions - June 20, 2018 Category: Molecular Biology Authors: Veríssimo G, Bast A, Weseler AR Tags: Chem Biol Interact Source Type: research

Penicillamine - not only a chelating agent but also a potent neuroprotector in the neonatal period.
PMID: 29935160 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 20, 2018 Category: Molecular Biology Authors: Lakatos L, Balla G, Pataki I Tags: Chem Biol Interact Source Type: research

Mitochondrial damage and apoptosis: Key features in BDE-153-induced hepatotoxicity.
ta DJ Abstract Brominated flame retardants are used in consumer goods to increase product resistance to fire and/or high temperatures. Polybrominated diphenyl ethers (PBDEs) are the most commonly employed class of brominated flame retardants because they are inexpensive and can effectively prevent flame from spreading. PBDEs are persistent, can bioaccumulate, are transported over long distances, and display toxicity. However, their toxic mechanisms of action have not been well established. Because mitochondria are recognized as the main energy-producing cell organelle and play a vital role in cellular function mai...
Source: Chemico-Biological Interactions - June 20, 2018 Category: Molecular Biology Authors: Pereira LC, Cabral Miranda LF, Franco-Bernardes MF, Tasso MJ, Duarte FV, Inácio Varela AT, Rolo AP, Marques Palmeira CM, Dorta DJ Tags: Chem Biol Interact Source Type: research

Two-sided action of Danshen on cytoprotective endogenous substances, epoxyeicosatrienoic acids.
PMID: 29935162 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 20, 2018 Category: Molecular Biology Authors: Yasar U Tags: Chem Biol Interact Source Type: research

COX-2 inhibition by celecoxib in epithelial ovarian cancer attenuates E-cadherin suppression through reduced Snail nuclear translocation.
Abstract Elevated cyclooxygenase-2 (COX-2) closely associates with tumor progression and distant metastasis in various human cancers. However, the role of COX-2 in epithelial ovarian cancer (EOC), and its mechanistic details, remain poorly understood. In the present study, we tested hypothesis that COX-2 induces loss of expression of E-cadherin, with resulting promotion of cancer cells' invasiveness in ovarian cancer. First, we observed an inverse relationship between COX-2 and E-cadherin expression as COX-2 was enhanced but E-cadherin was decreased in surgically-resected specimens of EOC. Depletion of COX-2, by c...
Source: Chemico-Biological Interactions - June 19, 2018 Category: Molecular Biology Authors: Wang YP, Wang QY, Li CH, Li XW Tags: Chem Biol Interact Source Type: research

Electrochemical remediation of amoxicillin: Detoxification and reduction of antimicrobial activity.
ra GA Abstract Amoxicillin (AMX) is one of the most commonly prescribed antibiotics around the world to treat and prevent several diseases in both human and veterinary medicine. Incomplete removal of AMX during wastewater treatment contributes to its presence in water bodies and drinking water. AMX is an emerging contaminant since its impact on the environment and human health remains uncertain. This contribution was aimed to evaluate the electrochemical oxidation (EO) of AMX using different anodes in tap water, NaCl or Na2SO4 solutions and to evaluate the potential toxicity of remaining AMX and its by-products on...
Source: Chemico-Biological Interactions - June 16, 2018 Category: Molecular Biology Authors: Brito LB, Garcia LF, Caetano MP, Lobón GS, Teles de Oliveira M, de Oliveira R, Sapateiro Torres IM, Yepez A, Vaz BG, Luque R, Grisolia CK, Valadares MC, de Souza Gil E, Rodrigues de Oliveira GA Tags: Chem Biol Interact Source Type: research

The inhibition, reactivation and mechanism of VX-, sarin-, fluoro-VX and fluoro-sarin surrogates following their interaction with HuAChE and HuBuChE.
In this study, the mechanisms of HuAChE and HuBChE inhibition by Me-P(O) (OPNP) (OR) [PNP = p-nitrophenyl; R = CH2CH3, CH2CH2F, OCH(CH3)2, OCH(CH3) (CH2F)] representing surrogates and fluoro-surrogates of VX and sarin were studied by in vitro kinetics and mass spectrometry. The in vitro measures showed that the VX- and fluoro-VX surrogates were relatively strong inhibitors of HuAChE and HuBChE (ki ∼ 105-106 M-1min-1) and underwent spontaneous and 2-PAM-mediated reactivation within 30 min. The sarin surrogates were weaker inhibitors of HuAChE and HuBChE (ki ∼ 104-105 M-1min-1), and in general d...
Source: Chemico-Biological Interactions - June 16, 2018 Category: Molecular Biology Authors: Chao CK, Balasubramanian N, Gerdes JM, Thompson CM Tags: Chem Biol Interact Source Type: research

Impact of diet on irinotecan toxicity in mice.
Abstract Irinotecan is highly effective in the treatment of metastatic colorectal cancer as well as many other cancers. However, irinotecan is known to cause severe diarrhea, which pose significant problems in patients undergoing irinotecan based chemotherapy. Dietary and herbal components have shown promise in improving gastrointestinal health. Therefore, we compared the effect of grain-based chow diet containing phytoestrogens and corn/alfalfa as fat source to purified diets containing either animal-derived fat source (lard) or plant-derived fat source (soybean oil) on irinotecan-induced toxicities in mice. The ...
Source: Chemico-Biological Interactions - June 15, 2018 Category: Molecular Biology Authors: Mallick P, Shah P, Ittmann MM, Trivedi M, Hu M, Gao S, Ghose R Tags: Chem Biol Interact Source Type: research

Biodistribution and toxicity assessment of intratumorally injected arginine-glycine-aspartic acid peptide conjugated to CdSe/ZnS quantum dots in mice bearing pancreatic neoplasm.
This study aimed to evaluate the biodistribution and toxicity of QD-RGD in mice prior to in vivo application. Mice with pancreatic neoplasms were intratumorally injected with varying doses of QD-RGD, and the biodistribution 0-24 h post injection was compared to that in control mice (intravenously injected with unconjugated QD). Various tissue samples were collected for toxicity analyses, which included inductively coupled plasma mass spectrometry (ICP-MS) to assess Cd2+ concentrations and hematoxylin-eosin staining for histopathological examination. Fluorescent imaging revealed relatively sufficient radiant efficiency in...
Source: Chemico-Biological Interactions - June 14, 2018 Category: Molecular Biology Authors: Li MM, Cao J, Yang JC, Shen YJ, Cai XL, Chen YW, Qu CY, Zhang Y, Shen F, Zhou M, Xu LM Tags: Chem Biol Interact Source Type: research

Hydroquinone exposure worsens the symptomatology of rheumatoid arthritis.
Abstract The genesis of rheumatoid arthritis (RA) is complex and dependent on genetic background and exposure to environmental xenobiotic. Indeed, smoking is associated to developing and worsening pre-existing RA. Nevertheless, the mechanisms and cigarette compounds involved in the harmful processes have not been elucidated. Here, we investigated if the exposure to hydroquinone (HQ), an abundant pro-oxidative compound of cigarette and benzene metabolite, could worsen the ongoing RA. Hence, collagen-induced arthritis (CIA) was induced in male Wistar rats by s.c. injection of 400 μg (200 μL) of bovine coll...
Source: Chemico-Biological Interactions - June 14, 2018 Category: Molecular Biology Authors: Heluany CS, Kupa LVK, Viana MN, Fernandes CM, Farsky SHP Tags: Chem Biol Interact Source Type: research

Monotropein attenuates ovariectomy and LPS-induced bone loss in mice and decreases inflammatory impairment on osteoblast through blocking activation of NF- κB pathway.
Monotropein attenuates ovariectomy and LPS-induced bone loss in mice and decreases inflammatory impairment on osteoblast through blocking activation of NF-κB pathway. Chem Biol Interact. 2018 Jun 14;: Authors: He YQ, Yang H, Shen Y, Zhang JH, Zhang ZG, Liu LL, Song HT, Lin B, Hsu HY, Qin LP, Han T, Xin HL, Zhang QY Abstract Estrogen deficiency and inflammation are known to play important roles in bone metabolism and occurrence of osteoporosis. Monotropein as an iridoid glycoside is reported to decrease estrogen deficiency-induced bone loss and inhibit inflammatory response in LPS-induced RAW 264...
Source: Chemico-Biological Interactions - June 14, 2018 Category: Molecular Biology Authors: He YQ, Yang H, Shen Y, Zhang JH, Zhang ZG, Liu LL, Song HT, Lin B, Hsu HY, Qin LP, Han T, Xin HL, Zhang QY Tags: Chem Biol Interact Source Type: research

Antineoplastic activity of mitomycin C formulated in nanoemulsions-based essential oils on HeLa cervical cancer cells.
In conclusion, combining MMC with NE-based ESSOs has increased the cytotoxic effect of the MMC on the HeLa cells with different mechanism of actions. PMID: 29908166 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 13, 2018 Category: Molecular Biology Authors: Alkhatib MH, Al-Otaibi WA, Wali AN Tags: Chem Biol Interact Source Type: research

Teroxirone motivates apoptotic death in tumorspheres of human lung cancer cells.
Abstract Therapy by targeting cancer stem cells (CSCs) is an eligible method to eradicate malignant human tumors. A synthetic triepoxide derivative, teroxirone, was reported effective against growth of human lung cancer cells by injuring cellular mitochondria functions. And yet it remains unclear if the residual but malicious CSCs can be effectively dissipated as a result of treatment. The current study further affirmed that teroxirone inhibited propagation of CSCs as enriched from NSCLC cells by inducing p53 that lead to ultimate apoptosis. More evidence supported that the reduced stemness of the spheroids was as...
Source: Chemico-Biological Interactions - June 13, 2018 Category: Molecular Biology Authors: Ni YL, Hsieh CH, Wang JP, Fang K Tags: Chem Biol Interact Source Type: research

Biochemical and histological effects of sub-chronic exposure to atrazine in crayfish Cherax destructor.
Abstract Atrazine (ATR) is a triazine herbicide banned in the European Union. It remains one of the most widely used herbicides in other parts of the world. Considering the scarcity of data on its possible harm to the environment and to human health, we assessed sub-chronic effects of a 14-day exposure at the environmentally relevant concentration of 6.86 μg/L and at 10% of the 96hLC50 (1.21 mg/L) in crayfish Cherax destructor and their recovery in a 14-day period in ATR-free water. Indicators assessed were behavior; hemolymph biochemical profile; oxidative and antioxidant parameters in gill, hepatopancreas...
Source: Chemico-Biological Interactions - June 13, 2018 Category: Molecular Biology Authors: Stara A, Kouba A, Velisek J Tags: Chem Biol Interact Source Type: research

Randomized clinical trial of a mucoadhesive formulation containing curcuminoids (Zingiberaceae) and Bidens pilosa Linn (Asteraceae) extract (FITOPROT) for prevention and treatment of oral mucositis - phase I study.
es MC Abstract Preclinical repeated-dose toxicity and efficiency studies developed by our group suggest the potential of FITOPROT in treating mucositis. This serious limiting side effect is observed at a rate of 40-100% in patients under antineoplastic therapy and despite different palliative measures and therapeutic agents have been investigated, still no therapy was completely successful. Therefore, this study aimed to establish the safety and recommended phase II dose of FITOPROT for the prevention and treatment of chemoradiotherapy-induced oral mucositis (OM) in patients with head and neck cancer. Twenty healt...
Source: Chemico-Biological Interactions - June 12, 2018 Category: Molecular Biology Authors: Xavier Dos Santos Filho E, Costa Arantes DA, Oton Leite AF, Batista AC, Francisco de Mendonça E, Marreto RN, Naves LN, Lima EM, Valadares MC Tags: Chem Biol Interact Source Type: research

Synthesis of newly functionalized 1,4-naphthoquinone derivatives and their effects on wound healing in alloxan-induced diabetic mice.
eto E Abstract Naphthoquinone derivatives have various pharmacological properties. Here, we describe the synthesis of new 1,4-naphthoquinone derivatives inspired by lawsone and β-lapachone and their effects on both migration of fibroblasts in vitro and dermal wound healing in diabetic mice. NMR and FTIR spectroscopy aided characterization of chemical composition and demonstrated the molecular variations after the synthesis of four different derivatives, namely 2-bromo-1,4-naphthoquinone (termed derivative S3), 2-N-phenylamino-1,4-naphthoquinone (derivative S5), 2-N-isonicotinoyl-hydrazide-1,4-naphthoquinone (...
Source: Chemico-Biological Interactions - June 11, 2018 Category: Molecular Biology Authors: Cardoso SH, de Oliveira CR, Guimarães AS, Nascimento J, de Oliveira Dos Santos Carmo J, de Souza Ferro JN, de Carvalho Correia AC, Barreto E Tags: Chem Biol Interact Source Type: research

Cannabidiol did not induce teratogenicity or neurotoxicity in exposed zebrafish embryos.
Abstract Cannabidiol (CBD) is a non-psychotomimetic compound of the Cannabis sativa that has been used for the treatment of severe epilepsy as well as other diseases of nervous system. However, toxicity studies of CBD have great relevance to guarantee the patients safety. In this context, morphological analyses of zebrafish can contribute to evaluate the teratogenic potential, as well as evaluation of acetylcholinesterase activity and motor activity of zebrafish are valuable tools to verify the neurotoxicity potential. In the present work, we use this methodology to test the toxicity of CBD to zebrafish embryos. N...
Source: Chemico-Biological Interactions - June 11, 2018 Category: Molecular Biology Authors: Valim Brigante TA, Abe FR, Zuardi AW, Hallak JEC, Crippa JAS, de Oliveira DP Tags: Chem Biol Interact Source Type: research

microRNA-199a/b-5p enhance imatinib efficacy via repressing WNT2 signaling-mediated protective autophagy in imatinib-resistant chronic myeloid leukemia cells.
Abstract Imatinib (IM) is a first-line therapeutic drug for chronic myeloid leukemia (CML), a hematological disease. Mutations in the BCR-ABL domain increase formation of IM resistance in CML. However, not all patients are BCR-ABL domain-mutant dependent. Investigating non-mutant mechanisms in the development of acquired IM resistance is a critical issue. We explored the mechanisms which influence IM efficacy and resistance in CML. Higher protective autophagy was identified in IM-resistant K562 (K562R) cells. Inhibition of autophagy by the inhibitors, chloroquine and 3-methyladenine, enhanced IM's efficacy in K562...
Source: Chemico-Biological Interactions - June 8, 2018 Category: Molecular Biology Authors: Chen PH, Liu AJ, Ho KH, Chiu YT, Anne Lin ZH, Lee YT, Shih CM, Chen KC Tags: Chem Biol Interact Source Type: research

From epidemiology to treatment: Aspirin's prevention of brain and breast-cancer and cardioprotection May associate with its metabolite gentisic acid.
CONCLUSIONS: GA highly merits to be studied in further animal models as an anticancer and chemoprotective drug. PMID: 29885857 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 7, 2018 Category: Molecular Biology Authors: Altinoz MA, Elmaci I, Cengiz S, Emekli-Alturfan E, Ozpinar A Tags: Chem Biol Interact Source Type: research

Removal of azo dye using Fenton and Fenton-like processes: Evaluation of process factors by Box-Behnken design and ecotoxicity tests.
Abstract The conventional treatment of textile effluents is usually inefficient in removing azo dyes and can even generate more toxic products than the original dyes. The aim of the present study was to optimize the process factors in the degradation of Disperse Red 343 by Fenton and Fenton-like processes, as well as to investigate the ecotoxicity of the samples treated under optimized conditions. A Box-Behnken design integrated with the desirability function was used to optimize dye degradation, the amount of residual H2O2 [H2O2residual], and the ecotoxicity of the treated samples (lettuce seed, Artemia salina, a...
Source: Chemico-Biological Interactions - June 6, 2018 Category: Molecular Biology Authors: Fernandes NC, Brito LB, Costa GG, Taveira SF, Cunha-Filho MSS, Oliveira GAR, Marreto RN Tags: Chem Biol Interact Source Type: research

NMDA and GABA receptor presence in rat heart mitochondria.
Abstract The purpose of this study is to demonstrate the presence of three more receptors in mitochondria. Two N-methyl-d-aspartate receptor (NMDAR) subunits (NR1 and NR2B) are found by protein immunoblot and immunogold labeling in mitochondria fraction isolated from rat heart. These data allow supposing NMDAR presence and functioning in the inner mitochondrial membrane. There are no signs of receptor presence obtained in heart tissue lysate, that indicates the receptor localization exactly in mitochondria. The possible receptor functions discussed are its participation in calcium transport and in excitation-metab...
Source: Chemico-Biological Interactions - June 5, 2018 Category: Molecular Biology Authors: Nesterov SV, Skorobogatova YA, Panteleeva AA, Pavlik LL, Mikheeva IB, Yaguzhinsky LS, Nartsissov YR Tags: Chem Biol Interact Source Type: research

Alisol A 24-acetate ameliorates nonalcoholic steatohepatitis by inhibiting oxidative stress and stimulating autophagy through the AMPK/mTOR pathway.
Abstract Alisol A 24-acetate (AA), a natural triterpenoid isolated from the traditional Chinese medicine Rhizoma Alismatis, has various therapeutic effects. We investigated the anti-nonalcoholic steatohepatitis (NASH) effect of AA and its underlying mechanisms in vitro and in vivo. C57BL/6 mice were fed a methionine and choline-deficient (MCD) diet for 4 weeks to induce NASH. The mice were simultaneously treated with a daily dose of AA (15, 30, and 60 mg kg-1, ig) for 4 weeks. On the last day, the animals were sacrificed and plasma and liver tissue were collected. Serum and liver tissue biochemical analyses an...
Source: Chemico-Biological Interactions - June 5, 2018 Category: Molecular Biology Authors: Wu C, Jing M, Yang L, Jin L, Ding Y, Lu J, Cao Q, Jiang Y Tags: Chem Biol Interact Source Type: research

Nanoceria acts as antioxidant in tumoral and transformed cells.
dez A Abstract Antioxidant/prooxidant properties of cerium oxide nanoparticles (nanoceria) have been reported. It has been proposed that this protective/adverse role would depend of the cell status. In tumoral cells, nanoceria would act as prooxidant, while in normal cells it would act as antioxidant. In this context our work aims to study the nanoceria antioxidant/prooxidant capacity in different tumoral cell lines, studying how cell origin (non-tumoral vs tumoral), or extracellular environment could affect its protective/adverse effect. We have determined the ability of nanoceria to reduce the levels of reactive...
Source: Chemico-Biological Interactions - June 4, 2018 Category: Molecular Biology Authors: Rubio L, Marcos R, Hernández A Tags: Chem Biol Interact Source Type: research

Antiproliferative effect of p-Coumaric acid targets UPR activation by downregulating Grp78 in colon cancer.
Abstract p-CA is a naturally occurring phenolic acid present in most plants and in all commonly consumed vegetables and fruits. Here we demonstrated the anti-cancer effect of the food borne phytochemical p-CA both in vitro and in vivo models of colon cancer using growth rate and tumor incidence as endpoints. Glucose regulated protein (GRP78) induction and UPR activation plays a key role in oncogenic progression, therefore increased dependence of cancer cells on these UPR signaling pathways for survival can be exploited for anti-cancer research. Hence we investigated the effect of p-CA on Grp78 a molecular chaperon...
Source: Chemico-Biological Interactions - June 4, 2018 Category: Molecular Biology Authors: Sharma SH, Rajamanickam V, Nagarajan S Tags: Chem Biol Interact Source Type: research

CHM-1, a novel microtubule-destabilizing agent exhibits antitumor activity via inducing the expression of SIRT2 in human breast cancer cells.
In this report, we investigated whether CHM-1, a novel synthetic antimitotic agent could be developed into a potent antitumor agent for treating human breast cancer. CHM-1 induced growth inhibition in MDA-MB-231, MDA-MB-453 and MCF-7 cells in a concentration-dependent manner. Importantly, CHM-1 is less toxic to normal breast (HBL-100) cells. CHM-1 interacted with tubulin, markedly inhibited tubulin polymerization, and disrupted microtubule organization. Proteins from control and CHM-1-treated animal tumor specimens were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Our results indicated tha...
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Liu CW, Lin YC, Hung CM, Liu BL, Kuo SC, Ho CT, Way TD, Hung CH Tags: Chem Biol Interact Source Type: research

Inhibitory Effects of Danshen components on CYP2C8 and CYP2J2.
Abstract The use of Chinese herbal medicines and natural products has become increasingly popular in both China and Western societies as an alternative medicine for the treatment of diseases or as a health supplement. Danshen, the dried root of Salvia miltiorrhiza (Fam.Labiatae), which is rich in phenolic acids and tanshinones, is a widely used herbal medicine for the treatment of cardio-cerebrovascular diseases. The goal of this study was to examine the inhibitory effects of fifteen components derived from Danshen on CYP2C8 and CYP2J2, which are expressed both in human liver and cardiovascular systems. Recombinan...
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Xu MJ, Jiang LF, Wu T, Chu JH, Wei YD, Aa JY, Wang GJ, Hao HP, Ju WZ, Li P Tags: Chem Biol Interact Source Type: research

Flavan enantiomers from Daphne giraldii selectively induce apoptotic cell death in p53-null hepatocarcinoma cells in vitro.
Abstract Hepatocellular carcinoma (HCC) is a primary malignant tumor with very poor prognosis. To search for more effective compounds for HCC treatment, two new pairs of flavan enantiomers, daphnegiranol C1/C2 (1a/1b) and daphnegiranol D1/D2 (2a/2b), were isolated from the stem bark and roots of Daphne giraldii by using chiral chromatography. MTT assay was applied to evaluate their cytotoxicity against three hepatocarcinoma cell lines (Hep3B, MHCC97H, HepG2) as well as a normal liver cell line L02. The results showed that these compounds preferred to inhibit the growth of Hep3B cells (p53 null). Among them, 2a/2b ...
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Yao GD, Sun Q, Song XY, Huang XX, Song SJ Tags: Chem Biol Interact Source Type: research

Cytochrome P450 mediated metabolic activation of chrysophanol.
Abstract Chrysophanol, a major anthraquinone component occurring in many traditional Chinese herbs, is accepted as important active component with various pharmacological actions such as antibacterial and anticancer activity. Previous studies demonstrated that exposure to chrysophanol induced cytotoxicity, but the mechanisms of the toxic effects remain unknown. In the present metabolism study, three oxidative metabolites (M1-M3, aloe-emodine, 7-hydroxychrysophanol, and 2-hydroxychrysophanol) and five GSH conjugates (M4-M8) were detected in rat and human liver microsomal incubations of chrysophanol supplemented wit...
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Sun Y, Xin X, Zhang K, Cui T, Peng Y, Zheng J Tags: Chem Biol Interact Source Type: research

Combined effects of cadmium and tetrabromobisphenol a (TBBPA) on development, antioxidant enzymes activity and thyroid hormones in female rats.
This study conducted a subchronic effects of cadmium and TBBPA on the development and antioxidative defense system as well as thyroid functions in female rats through single and combined exposure at environmentally relevant doses for a 20-day consecutive administration. Body indexes, histopathology, redox status, and thyroid hormones levels were assessed. Slower body weight gains and reduced ovary weight (20.8% and 32.4% for combined and single-Cd exposures, respectively) were observed with significant variation from controls in high dose treatments. Co-exposure resulted in a slight enhancement in TSH levels compared to co...
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Yu Y, Ma R, Yu L, Cai Z, Li H, Zuo Y, Wang Z, Li H Tags: Chem Biol Interact Source Type: research

Oxyresveratrol ameliorates nonalcoholic fatty liver disease by regulating hepatic lipogenesis and fatty acid oxidation through liver kinase B1 and AMP-activated protein kinase.
This study examined the effects of OXY on in vitro model of NAFLD in hepatocyte by the liver X receptor α (LXRα)-mediated induction of lipogenic genes and in vivo model in mice along with its molecular mechanism. OXY inhibited the LXRα agonists-mediated sterol regulatory element binding protein-1c (SREBP-1c) induction and expression of the lipogenic genes and upregulated the mRNA of fatty acid β-oxidation-related genes in hepatocytes, which is more potent than genistein and daidzein. OXY also induced AMP-activated protein kinase (AMPK) activation in a time-dependent manner. Moreover, AMPK activation ...
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Lee JH, Baek SY, Jang EJ, Ku SK, Kim KM, Ki SH, Kim CE, Park KI, Kim SC, Kim YW Tags: Chem Biol Interact Source Type: research

Mesalazine, an osteopontin inhibitor: The potential prophylactic and remedial roles in induced liver fibrosis in rats.
In conclusion, mesalazine could have a potential new indication as anti-fibrotic agent through limiting the oxidative damage and altering TNF-ɑ pathway as an anti-inflammatory drug with down-regulating TGF-β1, OPN, α-SMA and caspase-3 signaling pathways. PMID: 29738702 [PubMed - indexed for MEDLINE] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Ramadan A, Afifi N, Yassin NZ, Abdel-Rahman RF, Abd El-Rahman SS, Fayed HM Tags: Chem Biol Interact Source Type: research

High fat diet induced obesity is mitigated in Cyp3a-null female mice.
In conclusion, lack of Cyp3a has a positive effect on acclimation to a HFD in females as it improves weight gain, glucose response and ketosis. PMID: 29738703 [PubMed - indexed for MEDLINE] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - June 1, 2018 Category: Molecular Biology Authors: Kumar R, Litoff EJ, Boswell WT, Baldwin WS Tags: Chem Biol Interact Source Type: research

MicroRNA-1179 inhibits the proliferation, migration and invasion of human pancreatic cancer cells by targeting E2F5.
In this study we explored the potential of MicroRNA-1179 as the therapeutic target for the treatment of pancreatic cancer. The results of this study indicated that the expression of miR-1179 was significantly downregulated in the pancreatic cancer cell lines as compared to the normal pancreatic cells. To unveil the potential role of miR-1179, it was overexpressed in the pancreatic cancer cells. It was observed that ectopic expression of miR-1179 caused reduction in the proliferation of pancreatic cancer cells by triggering G0/G1 cell cycle arrest. Further, overexpression of miR-1179 caused inhibition of the cell migration ...
Source: Chemico-Biological Interactions - May 31, 2018 Category: Molecular Biology Authors: Lin C, Hu Z, Yuan G, Su H, Zeng Y, Guo Z, Zhong F, Jiang K, He S Tags: Chem Biol Interact Source Type: research

Metformin alleviated endotoxemia-induced acute lung injury via restoring AMPK-dependent suppression of mTOR.
Abstract Inflammation requires intensive metabolic support and modulation of the metabolic pathways might become a novel strategy to limit inflammatory injury. Recent studies have revealed the anti-inflammatory effects of the anti-diabetic reagent metformin, but the underlying mechanisms remain unclear. In the present study, the potential effects of metformin on endotoxemia-induced acute lung injury (ALI) and their relationship with the representative metabolic regulator, including AMPK, sirtuin 1 and mTOR, were investigated. The results indicated that treatment with metformin suppressed LPS-induced upregulation o...
Source: Chemico-Biological Interactions - May 31, 2018 Category: Molecular Biology Authors: Wu K, Tian R, Huang J, Yang Y, Dai J, Jiang R, Zhang L Tags: Chem Biol Interact Source Type: research

Ganoderic Acid A improves high fat diet-induced obesity, lipid accumulation and insulin sensitivity through regulating SREBP pathway.
Abstract Obesity and its major co-morbidity, type 2 diabetes, have been an alarming epidemic prevalence without an effective treatment available. Sterol regulatory element-binding proteins (SREBPs) are major transcription factors regulating the expression of genes involved in biosynthesis of cholesterol, fatty acid and triglyceride. Therefore, inhibition of SREBP pathway may be a useful strategy to treat obesity with type 2 diabetes. Here, we identify a small molecule, Ganoderic Acid A (GAA), inhibits the SREBP expression and decreases the cellular levels of cholesterol and fatty acid in vitro. GAA also ameliorate...
Source: Chemico-Biological Interactions - May 28, 2018 Category: Molecular Biology Authors: Zhu J, Jin J, Ding J, Li S, Cen P, Wang K, Wang H, Xia J Tags: Chem Biol Interact Source Type: research

Venlafaxine mitigates cisplatin-induced nephrotoxicity via down-regulating apoptotic pathway in rats.
In this study, the effect of venlafaxine against cisplatin-induced nephrotoxicity and bladder rings hypersensitivity towards acetylcholine were explored. Single injection of cisplatin (7 mg/kg, ip) in Sprague-Dawley rats instigated nephrotoxicity evidenced by hindering renal function (changes in kidney/body weight ratio, serum creatinine, BUN, albumin and urinary total protein levels which were supported by histopathology). In addition, cisplatin caused a profound oxidative stress, inflammation and apoptosis. Treatment with venlafaxine (50 mg/kg, po) managed to alleviate the nephrotoxicity indices and rehabilitate the ...
Source: Chemico-Biological Interactions - May 28, 2018 Category: Molecular Biology Authors: El-Kashef DH, Sharawy MH Tags: Chem Biol Interact Source Type: research

Impact of prepubertal exposure to dietary protocatechuic acid on the hypothalamus-pituitary-testicular axis in rats.
In conclusion, prepubertal exposure to PCA is safe and positively impacted reproductive function at sexual maturity in male rats. The observed beneficial effects of PCA is related to its anti-inflammatory and redox regulatory mechanisms. PMID: 29842870 [PubMed - as supplied by publisher] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - May 26, 2018 Category: Molecular Biology Authors: Adedara IA, Omole O, Okpara ES, Fasina OB, Ayeni MF, Ajayi OM, Busari EO, Farombi EO Tags: Chem Biol Interact Source Type: research