Subterminal hydroxyeicosatetraenoic acids: Crucial lipid mediators in normal physiology and disease states
In conclusion, elucidation of different roles of subterminal HETEs in normal and disease states leads to identification of novel therapeutic targets and development of new therapeutic modalities in different disease states. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - December 11, 2018 Category: Biochemistry Source Type: research

Methyl ferulic acid attenuates liver fibrosis and hepatic stellate cell activation through the TGF-β1/Smad and NOX4/ROS pathways
In conclusion, our results demonstrate that MFA attenuated liver fibrosis and hepatic stellate cell activation by inhibiting the TGF-β1/Smad and NOX4/ROS signalling pathways. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - December 11, 2018 Category: Biochemistry Source Type: research

A new pyrrole based small molecule from Tinospora cordifolia induces apoptosis in MDA-MB-231 breast cancer cells via ROS mediated mitochondrial damage and restoration of p53 activity
In this study, we investigated the effect of the natural compound, Bis(2- ethyl hexyl) 1H-pyrrole-3,4-dicarboxylate (TCCP), purified from Tinospora cordifolia on MDA-MB-231, a TNBC cell line. The pro-apoptotic nature of TCCP on MDA-MB-231 was determined by assessing various apoptotic markers. ROS generation, intracellular calcium, mitochondrial membrane potential (ΔΨm), MPTP, cardiolipin peroxidation and caspase activity were determined fluorometrically. BAX, BCL-2, cytochrome c, caspases, and p53 protein expressions were determined by immunoblotting. Further, the effect of TCCP on DNA and cell death was determin...
Source: Chemico Biological Interactions - December 10, 2018 Category: Biochemistry Source Type: research

Protein S-glutathionylation: The linchpin for the transmission of regulatory information on redox buffering capacity in mitochondria
Publication date: Available online 8 December 2018Source: Chemico-Biological InteractionsAuthor(s): Adrian Young, Robert Gill, Ryan J. MaillouxAbstractProtein S-glutathionylation reactions are a ubiquitous oxidative modification required to control protein function in response to changes in redox buffering capacity. These reactions are rapid and reversible and are, for the most part, enzymatically mediated by glutaredoxins (GRX) and glutathione S-transferases (GST). Protein S-glutathionylation has been found to control a range of cell functions in response to different physiological cues. Although these reactions occur thr...
Source: Chemico Biological Interactions - December 8, 2018 Category: Biochemistry Source Type: research

Editorial: XX Brazilian Congress of Toxicology
Publication date: Available online 4 December 2018Source: Chemico-Biological InteractionsAuthor(s): Gisele Augusto Rodrigues de Oliveira, Elisa Raquel Anastácio Ferraz Avelino (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - December 5, 2018 Category: Biochemistry Source Type: research

Carbon disulfide induces embryo loss by perturbing the expression of the mTOR signalling pathway in uterine tissue in mice
In this study, we focus on the effect of alterations in the expression of the AKT/AMPK/mTOR signalling pathway in mouse uterine tissue after embryo loss induced by harmful environmental exposure to carbon disulfide (CS2). CS2 is a material used in certain production processes, and women are sometimes exposed to it in occupational settings. We created an animal model of gestating mice exposed to CS2 on gestation days 3 (GD3), 4 (GD4), 5 (GD5) and 6 (GD6) with various corresponding endpoints after the exposure. The uterine tissue was collected according to the endpoint time series to detect the expression levels of mTOR, p-m...
Source: Chemico Biological Interactions - December 4, 2018 Category: Biochemistry Source Type: research

TLR5 silencing reduced hyperammonaemia-induced liver injury by inhibiting oxidative stress and inflammation responses via inactivating NF-κB and MAPK signals
ConclusionTLR5 silencing reduced HA-induced liver injury by inhibiting hepatocyte apoptosis, oxidative stress and inflammation responses via inactivating NF-κB and MAPK signals, deepening our understanding on the molecular mechanism of HA-induced liver injury and providing a potential therapeutic target for alleviating liver injury. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - December 1, 2018 Category: Biochemistry Source Type: research

Pharmacological activities of antroquinonol- Mini review
Publication date: 5 January 2019Source: Chemico-Biological Interactions, Volume 297Author(s): Venkatachalam Angamuthu, M. Shanmugavadivu, G. Nagarajan, Bharath Kumar VelmuruganAbstractAntrodia camphorata is an expensive mushroom that grows on the inner cavity of an endangered native tree of Taiwan namely Cinnamomum kanehirai Hayata. It is used as a traditional medicine in Taiwan and has several health benefits including free radical scavenging, anti-inflammatory, antimicrobial, hepatoprotective, neuroprotective, antidiabetic, and free radical-induced DNA damage protecting activities. Antroquinonol is a tetrahydro ubiquinon...
Source: Chemico Biological Interactions - November 29, 2018 Category: Biochemistry Source Type: research

Oenothein B inhibits human non-small cell lung cancer A549 cell proliferation by ROS-mediated PI3K/Akt/NF-κB signaling pathway
Publication date: 25 January 2019Source: Chemico-Biological Interactions, Volume 298Author(s): Xiaodong Pei, Junsong Xiao, Guijiang Wei, Yumeng Zhang, Feng Lin, Zhaocheng Xiong, Lan Lu, Xueli Wang, Guangfu Pang, Yan Jiang, Lihe JiangAbstractOenothein B has a wide range of biological activities. The present study probed into the underlying mechanism on how Oenothein B inhibits the proliferation of a lung cancer line A549. Our results showed that Oenothein B effectively inhibited the proliferation of A549 cells by inducing apoptosis and arresting cells at G1 stage. Furthermore, Oenothein B not only increased the level of i...
Source: Chemico Biological Interactions - November 29, 2018 Category: Biochemistry Source Type: research

Anti-proliferative and cytotoxic activities of the flavonoid isoliquiritigenin in the human neuroblastoma cell line SH-SY5Y
Publication date: Available online 28 November 2018Source: Chemico-Biological InteractionsAuthor(s): Stephane J.de M. Escobar, Genevieve M. Fong, Sheila M.B. Winnischofer, Martin Simone, Lenka Munoz, Joanne M. Dennis, Maria Eliane M. Rocha, Paul K. WittingAbstractNeuroblastoma is a common childhood cancer with high mortality. We evaluated the capacity of the flavonoid, isoliquiritigenin (4,2’,4’-trihydroxychalcone; ISL) to inhibit cellular proliferation and migration in the human neuroblastoma cell line SH-SY5Y. Incubation of cultured SH-SY5Y cells with 20–100 μM ISL decreased cell confluency (15&nda...
Source: Chemico Biological Interactions - November 28, 2018 Category: Biochemistry Source Type: research

Azure B affects amyloid precursor protein metabolism in PS70 cells
Publication date: Available online 28 November 2018Source: Chemico-Biological InteractionsAuthor(s): Kevser Biberoglu, Melike Yuksel, Ozden TacalAbstractAlzheimer's disease (AD), the most common form of dementia, is characterized by abundant deposition of amyloid-β (Aβ) peptide that is the result of sequential cleavage of amyloid precursor protein (APP) by β-secretase and γ-secretase. Several studies have documented that inhibition of Aβ peptide synthesis or facilitating its degradation is one of the attractive therapeutic strategies in AD. Methylene blue (MethB), which has recently been investiga...
Source: Chemico Biological Interactions - November 28, 2018 Category: Biochemistry Source Type: research

Protective role of chrysin on thioacetamide-induced hepatic encephalopathy in rats
This study aimed to investigate the possible neuroprotective effect of chrysin, a natural flavenoid on thioacetamide (TAA)-induced hepatic encephalopathy in rats. Also the effect of chrysin on motor impairment, cognitive deficits, oxidative stress, neuroinflammation, apoptosis and histopathological damage was assessed. HE was induced in Wistar rats by intraperitoneal (i.p.) injection of TAA (200 mg/kg) for three alternative days. Normal and control groups received the vehicle for 21 days. Chrysin was administered orally for 21 days (25, 50, 100 mg/kg) and starting from day 17, rats received i.p. dose of TAA (200 mg/k...
Source: Chemico Biological Interactions - November 28, 2018 Category: Biochemistry Source Type: research

Changes in physicochemical properties of kidney cells membrane as a consequence of hypertension and treatment of hypertensive rats with FAAH inhibitor
In conclusion, URB597 given to individuals with hypertension, particularly with secondary hypertension, enhancing some disturbances in electric and physicochemical properties of kidney cells observed in hypertension what may lead to additional kidney disorders. Therefore, further researches are necessary. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - November 28, 2018 Category: Biochemistry Source Type: research

A novel exposure system generating nebulized aerosol of sulfur mustard in comparison to the standard submerse exposure
Publication date: Available online 28 November 2018Source: Chemico-Biological InteractionsAuthor(s): Amelie Tsoutsoulopoulos, Markus Siegert, Harald John, Tabea Zubel, Aswin Mangerich, Annette Schmidt, Harald Mückter, Thomas Gudermann, Horst Thiermann, Dirk Steinritz, Tanja PoppAbstractInhalation of the chemical warfare agent sulfur mustard (SM) is associated with severe acute and long-term pulmonary dysfunctions and health effects. The still not completely elucidated molecular toxicology and a missing targeted therapy emphasize the need for further research. However, appropriate human data are extremely rare. In vivo...
Source: Chemico Biological Interactions - November 28, 2018 Category: Biochemistry Source Type: research

Oleanolic acid promotes orofacial antinociception in adult zebrafish (Danio rerio) through TRPV1 receptors
This study aimed to evaluate the antinociceptive effect of oleanolic acid (AO) using adult zebrafish models of orofacial pain. Acute nociception was induced by formalin, capsaicin, cinnamaldehyde, menthol, acidified saline or glutamate (cutaneous modes) and hypertonic saline (corneal model). In another set of experiments, animals were pre-treated with naloxone, L-NAME, methylene blue, ketamine, camphor, HC-030031, mefenamic acid, ruthenium red or amiloride to investigate the mechanism of antinociception. The involvement of central afferent C-fibers was also investigated. A molecular docking was performed using the TRPV1 ch...
Source: Chemico Biological Interactions - November 27, 2018 Category: Biochemistry Source Type: research

Decreased 8-oxoguanine DNA glycosylase 1 (hOGG1) expression and DNA oxidation damage induced by Cr (VI)
Publication date: Available online 27 November 2018Source: Chemico-Biological InteractionsAuthor(s): Hailing Xia, Shibo Ying, Lingfang Feng, Haiming Wang, Chunji Yao, Tao Li, Yixiao Zhang, Sanjun Fu, Donger Ding, Xinnian Guo, Yan Tong, Xiaofeng Wang, Zhijian Chen, Zhaoqiang Jiang, Xing Zhang, Bernardo Lemos, Jianlin LouAbstractOccupational exposure to Cr (VI) can cause DNA damage, genetic instability and elevate the risk of cancer. Here we investigated Cr (VI)-induced DNA damage and 8-oxoguanine DNA glycosylase 1 (hOGG1) gene expression in electroplating workers. The hOGG1 gene encodes a DNA repair enzyme that is crucial i...
Source: Chemico Biological Interactions - November 27, 2018 Category: Biochemistry Source Type: research

Interaction of the anti-tuberculous drug bedaquiline with artificial membranes and rat erythrocytes
Publication date: Available online 26 November 2018Source: Chemico-Biological InteractionsAuthor(s): Konstantin N. Belosludtsev, Nikita V. Penkov, Kirill S. Tenkov, Eugeny Yu Talanov, Natalia V. Belosludtseva, Alexey V. Agafonov, Anastasia E. Stepanova, Vlada S. Starinets, Olga V. Vashchenko, Sergey V. Gudkov, Mikhail V. DubininAbstractBedaquiline (BDQ) is a new drug from the family of diarylquinolines, which has a potent bactericidal activity against Mycobacterium tuberculosis. This paper has examined the interaction of BDQ with model membranes (liposomes and BLM) and rat erythrocytes. It was shown that BDQ (1–10 ...
Source: Chemico Biological Interactions - November 26, 2018 Category: Biochemistry Source Type: research

Acetaminophen absorption and metabolism in an intestine/liver microphysiological system
This study describes the characterization of pharmacokinetic (PK) properties of acetaminophen (APAP) in the Two-Organ-Chip platform (2-OC), a two-chamber device able to cultivate 3D tissues under flow. The APAP intestinal absorption and hepatic metabolism were emulated by human intestine and liver equivalents respectively. The intestinal barrier was produced using Caco-2 and HT-29 cells. The liver spheroids were produced with HepaRG and HHSTeC cells. Cell viability and toxicity were assessed by MTT assay, histology, confocal immunohistochemistry, and multiparametric high content analysis. Gene expression of intestine and...
Source: Chemico Biological Interactions - November 26, 2018 Category: Biochemistry Source Type: research

Disturbance of energetic homeostasis and oxidative damage provoked by trichlorfon as relevant toxicological mechanisms using silver catfish as experimental model
Publication date: Available online 24 November 2018Source: Chemico-Biological InteractionsAuthor(s): Matheus D. Baldissera, Carine F. Souza, Sharine N. Descovi, Renato Zanella, Osmar D. Prestes, Antonio F.I.M. de Matos, Aleksandro S. da Silva, Bernardo BaldisserottoAbstractRecent evidences have suggested the involvement of phosphoryl transfer, catalyzed by creatine kinase (CK), adenylate kinase (AK) and pyruvate kinase (PK), to metabolic alterations and impairment of bioenergetics homeostasis linked to adenosine triphosphate (ATP) production, and utilization during exposure to pesticides. It is recognized that sublethal co...
Source: Chemico Biological Interactions - November 24, 2018 Category: Biochemistry Source Type: research

Neuroprotective role of hyperforin on aluminum maltolate-induced oxidative damage and apoptosis in PC12 cells and SH-SY5Y cells
Publication date: Available online 24 November 2018Source: Chemico-Biological InteractionsAuthor(s): Haoran Wang, Bing Shao, Hongyan Yu, Feibo Xu, Peiyan Wang, Kaiyuan Yu, Yanfei Han, Miao Song, Yanfei Li, Zheng CaoAbstractMany reports demonstrated that aluminum maltolate (Almal) has potential toxicity to human and animal. Our study has demonstrated that Almal can induce oxidative damage and apoptosis in PC12 cells and SH-SY5Y Cells, two in vitro models of neuronal cells. Hyperforin (HF) is a well-known antioxidant, anti-inflammatory, anti-amyloid and anti-depressant compound extracted from Hypericum perforatum extract. ...
Source: Chemico Biological Interactions - November 24, 2018 Category: Biochemistry Source Type: research

8-Cetylcoptisine, a new coptisine derivative, induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest in human A549 cells
Publication date: Available online 22 November 2018Source: Chemico-Biological InteractionsAuthor(s): Bing Han, Pu Jiang, Heshan Xu, Wuyang Liu, Jian Zhang, Siqi Wu, Liangyu Liu, Wenyu Ma, Xuegang Li, Xiaoli YeAbstractLung cancer is the worldwide leading cause of cancer-related death. Here, we described the synthesis and the anticancer activity of a novel coptisine derivative 8-cetylcoptisine (CCOP) on lung carcinoma in vitro and in vivo. CCOP inhibited the cell viability of A549, BGC-823, MDA-MB-231, HCT-116 and HepG2 cell lines. In A549 cells, CCOP induced apoptosis, G0/G1 cell cycle arrest and decreased mitochondrial m...
Source: Chemico Biological Interactions - November 23, 2018 Category: Biochemistry Source Type: research

Pyrrolizidine alkaloid-induced alterations of prostanoid synthesis in human endothelial cells
Publication date: Available online 19 November 2018Source: Chemico-Biological InteractionsAuthor(s): Johanna Ebmeyer, Jessica Behrend, Mario Lorenz, Georgia Günther, Raymond Reif, Jan G. Hengstler, Albert Braeuning, Alfonso Lampen, Stefanie Hessel-PrasAbstractPyrrolizidine alkaloids (PA) are a group of secondary plant metabolites belonging to the most widely distributed natural toxins. PA intoxication of humans leads to severe liver damage, such as hepatomegaly, hepatic necrosis, fibrosis and cirrhosis. An acute consequence observed after ingestion of high amounts of PA is veno-occlusive disease (VOD) where the hepati...
Source: Chemico Biological Interactions - November 21, 2018 Category: Biochemistry Source Type: research

Mahanine induces apoptosis, cell cycle arrest, inhibition of cell migration, invasion and PI3K/AKT/mTOR signalling pathway in glioma cells and inhibits tumor growth in vivo
Publication date: Available online 20 November 2018Source: Chemico-Biological InteractionsAuthor(s): Maohua Chen, Xiangqian Yin, Chuan Lu, Xiaodong Chen, Huajun Ba, Jianyong Cai, Jun SunAbstractGliomas are among the most frequent types of primary malignancies in the central nervous system. The main treatment for glioma includes surgical resection followed by a combination of radiotherapy and chemotherapy. Despite the availability of several treatments, the average survival for patients with glioma at advanced stages still remains 16 months only. Therefore, there is an urgent need to look for novel and more efficient drug c...
Source: Chemico Biological Interactions - November 21, 2018 Category: Biochemistry Source Type: research

Omeprazole protects against cisplatin-induced nephrotoxicity by alleviating oxidative stress, inflammation, and transporter-mediated cisplatin accumulation in rats and HK-2 cells
Publication date: Available online 16 November 2018Source: Chemico-Biological InteractionsAuthor(s): Huan Gao, Sixi Zhang, Tingting Hu, Xiaoyu Qu, Jinghui Zhai, Yueming Zhang, Lina Tao, Jianyuan Yin, Yanqing SongAbstractThe present study assessed the therapeutic potential of omeprazole (OME), the most commonly prescribed proton pump inhibitor (PPI) used to treat gastroesophageal hyperacidity, against cisplatin (CP)-induced toxicity in human renal tubular HK-2 cells and rat kidneys. Herein, we observed that exposure of HK-2 cells to OME reversed the injury caused by CP, including enhancing cell viability and alleviating...
Source: Chemico Biological Interactions - November 17, 2018 Category: Biochemistry Source Type: research

Oenothein B induced human non-small cell lung cancer A549 cell death by ROS-mediated PI3K/Akt/NF-κB signaling pathway
This study assessed the possible mechanism of Oenothein B on lung cancer A549 cell. The results showed that Oenothein B effectively inhibited the proliferation of A549 cells by inducing apoptosis, arrested cell at G1 stage. On the one hand, Oenothein B not only increased the level of intracellular reactive oxygen species (ROS), but induced some apoptotic-related protein expression (cleavage caspase-3, PARP, cytochrome c level in the cytoso, Bax). Moreover, ROS inhibitor and PI3K agonist exhibited significant protection against cell death by Oenothein B. ROS inhibitor significantly abrogated the activation of caspase3/7...
Source: Chemico Biological Interactions - November 16, 2018 Category: Biochemistry Source Type: research

Tocolytic activity of the Lippia alba essential oil and its major constituents, citral and limonene, on the isolated uterus of rats
The objective of this study was to investigate the mechanism of action of the L. alba essential oil (EOLa) and its major constituents citral and limonene, on isolated rat uterus muscle. To evaluate the EOLa, citral and limonene effect, cumulative concentrations curves for EOLa and citral (1–600 μg/mL) and for limonene (1–1200 μg/mL) were constructed from contractions of rat uterine strips under a 1 g tension. EOLa, citral and limonene dose-dependently relaxed myometrial preparations pre-contracted with 60 mM KCl, 10-2 IU/mL oxytocin, serotonin (10 μM), or ACh (10 μM). The results demonst...
Source: Chemico Biological Interactions - November 15, 2018 Category: Biochemistry Source Type: research

Altered electrical properties with controlled copper doping in ZnO nanoparticles infers their cytotoxicity in macrophages by ROS induction and apoptosis
Publication date: Available online 10 November 2018Source: Chemico-Biological InteractionsAuthor(s): Bikram Keshari Das, Suresh K. Verma, Tanushree Das, Pritam Kumar Panda, Kajal Parashar, Mrutyunjay Suar, S.K.S. ParasharAbstractThe present study reports the regulation of cytotoxicity of Cu doped ZnO nanoparticles in macrophages (RAW 264.7) due to altered physiochemical properties changes like electrical properties by controlled doping of Cu in ZnO. Cu-doped ZnO nanoparticles were prepared by High Energy Ball Milling technique (HEBM) and formed single phase Zn1-xCuxO (x = 0.0, 0.01, 0.02, 0.03) were called as pure ZnO,...
Source: Chemico Biological Interactions - November 11, 2018 Category: Biochemistry Source Type: research

Lactic acid induced microRNA-744 enhances motility of SiHa cervical cancer cells through targeting ARHGAP5
Publication date: Available online 10 November 2018Source: Chemico-Biological InteractionsAuthor(s): Chao Li, Linyan Jia, Yongsheng Yu, Liping JinAbstractHigh-risk (hr) human papillomaviruses (HPV) infection and integration has caused the majority of cervical cancer, of which E6 and E7 oncogenes are invariably retained and expressed to immortalize cells probably via affecting cell migration and invasion, and tumor metastasis. However, the underlying mechanism that mediates the procedure such as motility of cervical cancer cells within the tumor microenvironment is not well understood. Herein, we examined one possible facto...
Source: Chemico Biological Interactions - November 11, 2018 Category: Biochemistry Source Type: research

A synthetic chalcone derivative, 2-hydroxy-3′,5,5′-trimethoxychalcone (DK-139), triggers reactive oxygen species-induced apoptosis independently of p53 in A549 lung cancer cells
In this study, we found that DK-139 activated DNA damage responses, as was revealed by the accumulation of the tumor suppressor p53 and the phosphorylation of histone H2AX at Ser139 (called γ-H2AX), which are hallmarks of DNA damage responses. The occurrence of DK-139-induced DNA damage was confirmed through single-cell gel electrophoresis (comet tail assay). Interestingly, using p53-null HCT116 cells revealed that p53 was not involved in DK-139-induced apoptosis. Instead, we found that DK-139 increased the production of ROS, which led to the processing of caspase-2, BH3 interacting-domain death agonist (BID), casp...
Source: Chemico Biological Interactions - November 6, 2018 Category: Biochemistry Source Type: research

The possible ameliorative effect of simvastatin versus sulfasalazine on acetic acid induced ulcerative colitis in adult rats
ConclusionSIM has anti-inflammatory, cytoprotective and antioxidants effects that are not directly related to its cholesterol lowering activity against AA induced colitis this makes it a new therapeutic target for UC. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - November 6, 2018 Category: Biochemistry Source Type: research

Amyloid-β1-42 dynamically regulates the migration of neural stem/progenitor cells via MAPK-ERK pathway
Publication date: Available online 3 November 2018Source: Chemico-Biological InteractionsAuthor(s): Zhu Wang, Yantian Chen, Xueyi Li, Pinky Sultana, Ming Yin, Zejian WangAbstractNeural stem/progenitor cell (NSPC) based therapy represents an attractive treatment for Alzheimer's disease (AD), the most common neurodegenerative disorder with no effective treatment to date. This can be achieved by stimulating endogenous NSPCs and/or administrating exogenously produced NSPCs. Successful repair requires the migration of NSPCs to the loci where neuronal loss occurs, differentiation and integration into neural networks. However, th...
Source: Chemico Biological Interactions - November 4, 2018 Category: Biochemistry Source Type: research

Necrosulfonamide – Unexpected effect in the course of a sulfur mustard intoxication
Publication date: Available online 2 November 2018Source: Chemico-Biological InteractionsAuthor(s): Georg Menacher, Frank Balszuweit, Simon Lang, Horst Thiermann, Kai Kehe, Thomas Gudermann, Annette Schmidt, Dirk Steinritz, Tanja PoppAbstractAlthough its first military use in Ypres was 100 years ago, no causal therapy for sulfur mustard (SM) intoxications exists so far. To improve the therapeutic options for the treatment of SM intoxications, we developed a co-culture of keratinocytes (HaCaT cells) and immunocompetent cells (THP-1 cells) to identify potential substances for further research. Here, we report on the influe...
Source: Chemico Biological Interactions - November 3, 2018 Category: Biochemistry Source Type: research

Cytotoxicity of benzophenone-3, an organic ultraviolet filter, caused by increased intracellular Zn2+ levels in rat thymocytes
Publication date: Available online 2 November 2018Source: Chemico-Biological InteractionsAuthor(s): Hidekazu Utsunomiya, Riko Hiraishi, Kouji Kishimoto, Sarii Hamad, Shin Abe, Yusuke Bekki, Norio KamemuraAbstractBenzophenone-3 (BP-3) is often used as an ultraviolet (UV) light filter in sunscreen products. Although BP-3 protects the human skin and hair from damage caused by excessive UV radiation, it is reported to exhibit toxic effects in human. However, the cytotoxicity of BP-3 on various cells is still not well understood. In the present study, the cytotoxicity of BP-3 against rat thymocytes was evaluated using a flow cy...
Source: Chemico Biological Interactions - November 3, 2018 Category: Biochemistry Source Type: research

Pro- and antioxidant activity of chromium(III), iron(III), molybdenum(III) or nickel(II) and their mixtures
Publication date: Available online 30 October 2018Source: Chemico-Biological InteractionsAuthor(s): Sylwia Terpilowska, Andrzej Krzysztof SiwickiAbstractThe aim of this study was to examine the effect of chromium(III), iron(III), molybdenum(III) and nickel(II) and their combinations on pro- and antioxidant activity in mouse embryo fibroblasts and liver cancer cells.The present study shows that chromium(III), iron(III), nickel(II) and molybdenum(III) induce oxidative stress. In the case of chromium(III), nickel(II) and molybdenum(III) the intracellular ROS were dominant. However, in the case of iron(III) MDA was dominant - ...
Source: Chemico Biological Interactions - October 30, 2018 Category: Biochemistry Source Type: research

Differential proteome analysis of rat plasma after diisopropyl fluorophosphate (DFP) intoxication, a surrogate of nerve agent sarin
Publication date: Available online 30 October 2018Source: Chemico-Biological InteractionsAuthor(s): Kalyani Chaubey, Syed Imteyaz Alam, Chandra Kant Waghmare, Lokendra Singh, Nalini Srivastava, Bijoy K. BhattacharyaAbstractDiisopropyl fluorophosphate (DFP), a surrogate of nerve agent sarin, is an organophosphorus (OP) compound which inhibits neuronal enzyme acetylcholinesterase (AChE). Exposure of this compound leads to a wide range of toxic symptoms and survivors may exhibit long term neurotoxicity related to cognitive and memory defects. Due to ease of availability and similar mechanism of action to other highly toxic ne...
Source: Chemico Biological Interactions - October 30, 2018 Category: Biochemistry Source Type: research

Nothofagin suppresses mast cell-mediated allergic inflammation
In this study, we assessed the inhibitory effect of nothofagin on allergic inflammation using cultured/isolated mast cells and an anaphylaxis mouse model. Nothofagin treatment prevented histamine and β-hexosaminidase release by reducing the influx of calcium into the cytosol in a concentration-dependent manner. Nothofagin also inhibited the gene expression and secretion of pro-inflammatory cytokines such as tumor necrosis factor-α and interleukin-4 by downregulating the phosphorylation of Lyn, Syk, Akt and nuclear translocation of nuclear factor-κB. To confirm these effects of nothofagin in vivo, we used a...
Source: Chemico Biological Interactions - October 29, 2018 Category: Biochemistry Source Type: research

Acrylamide induces adipocyte differentiation and obesity in mice
In this study, ACR treatment dramatically increased the accumulation of lipid droplets. We also examined expression levels of peroxisome proliferator-activated receptors γ (PPARγ), CCAAT enhancer binding protein α (c/EBPα), and CCAAT enhancer binding protein β (c/EBPβ) as adipogenic transcription factors for adipocyte differentiation. They were dose-dependently increased by treatment of ACR. Moreover, effects of ACR on mitogen-activated protein kinases (MAPKs) and 5′ AMP-activated protein kinase (AMPK)-Acetyl-CoA carboxylase (ACC) activation were investigated. Results also showed tha...
Source: Chemico Biological Interactions - October 27, 2018 Category: Biochemistry Source Type: research

Cr (VI) induces crosstalk between apoptosis and autophagy through endoplasmic reticulum stress in A549 cells
In this study, the role of endoplasmic reticulum (ER) stress in Cr (VI)-induced crosstalk of apoptosis and autophagy was investigated. Cr (VI) resulted in ER stress by upregulating the expression of GRP78 and p-PERK. 4-Phenylbutyric acid (4PBA), an inhibitor of ER stress, reduced both Cr (VI)-induced apoptosis and autophagy, suggesting that ER stress played an important role in Cr (VI)-induced apoptosis and autophagy in A549 cells. Furthermore, Cr (VI)-induced apoptosis preceded autophagy. Z-VAD-FMK, the suppressor of apoptosis, repressed Cr (VI)-induced autophagy. Pretreatment with 3-MA, the inhibitor of autophagy, incr...
Source: Chemico Biological Interactions - October 26, 2018 Category: Biochemistry Source Type: research

Identification of nafamostat mesilate as an inhibitor of the fat mass and obesity-associated protein (FTO) demethylase activity
Publication date: Available online 25 October 2018Source: Chemico-Biological InteractionsAuthor(s): Xinxin Han, Ning Wang, Junya Li, Ying Wang, Ruiyong Wang, Junbiao ChangAbstractNafamostat mesilate is a serine protease inhibitor and used for the treatment of pancreatitis and cancers. The fat mass and obesity-associated protein (FTO) was identified to be a demethylase and played an important role in physiological processes. The aim of this study was to examine the inhibition of Nafamostat mesilate on FTO demethylase activity. A combination of thermodynamic and enzymatic activity studies provides an insight into the recogni...
Source: Chemico Biological Interactions - October 26, 2018 Category: Biochemistry Source Type: research

Hydrophilic scaffolds of oxime as the potent catalytic inactivator of reactive organophosphate
Publication date: Available online 25 October 2018Source: Chemico-Biological InteractionsAuthor(s): Shengzhuang Tang, Pamela T. Wong, Jayme Cannon, Kelly Yang, Sierra Bowden, Somnath Bhattacharjee, Jessica J. O'Konek, Seok Ki ChoiAbstractDespite its efficacy as a skin decontaminant of reactive organophosphates (OP), Dekon 139—a potassium salt of 2,3-butanedione monooxime (DAM)—is associated with adverse events related to percutaneous absorption largely due to its small size and lipophilicity. In order to address this physicochemical issue, we synthesized and evaluated the activity of a focused library of 14 hyd...
Source: Chemico Biological Interactions - October 26, 2018 Category: Biochemistry Source Type: research

Beta-caryophyllene protects against diet-induced dyslipidemia and vascular inflammation in rats: Involvement of CB2 and PPAR-γ receptors
Publication date: 5 January 2019Source: Chemico-Biological Interactions, Volume 297Author(s): Dareen A. Youssef, Hassan M. El-Fayoumi, Mona F. MahmoudAbstractBeta-caryophyllene (BCP) is a phytocannabinoid possessing selective agonistic activity to cannabinoid type-2 receptors (CB2R) and peroxisome proliferator-activated receptors-α (PPAR-α). However, few studies reported the contribution of PPAR-γ receptors in BCP effects. The aim of this study was to investigate the BCP effects on diet-induced dyslipidemia and vascular inflammation as well as the involvement of CB2R and PPAR-γ receptors. Wistar rat...
Source: Chemico Biological Interactions - October 26, 2018 Category: Biochemistry Source Type: research

Pharmalogical activities of antroquinonol- Mini review
Publication date: 5 January 2019Source: Chemico-Biological Interactions, Volume 297Author(s): Venkatachalam Angamuthu, M. Shanmugavadivu, G. Nagarajan, Bharath Kumar VelmuruganAbstractAntrodia camphorata is an expensive mushroom that grows on the inner cavity of an endangered native tree of Taiwan namely Cinnamomum kanehirai Hayata. It is used as a traditional medicine in Taiwan and has several health benefits including free radical scavenging, anti-inflammatory, antimicrobial, hepatoprotective, neuroprotective, antidiabetic, and free radical-induced DNA damage protecting activities. Antroquinonol is a tetrahydro ubiquinon...
Source: Chemico Biological Interactions - October 26, 2018 Category: Biochemistry Source Type: research

MicroRNA-454 contributes to sustaining the proliferation and invasion of trophoblast cells through inhibiting Nodal/ALK7 signaling in pre-eclampsia
In this study, we aimed to investigate the potential role and underlying mechanism of miR-454 in regulating trophoblast cell proliferation and invasion in vitro. We found that miR-454 expression was significantly decreased in placental tissues from PE patients compared to controls. Transfection of miR-454 mimics promoted the proliferation, reduced the apoptosis, and increased invasion of trophoblast cells, while transfection of miR-454 inhibitor showed opposite effects. Bioinformatics analysis showed that activin receptor-like kinase 7 (ALK7) was a potential target gene of miR-454. Dual-luciferase reporter assay showed miR...
Source: Chemico Biological Interactions - October 25, 2018 Category: Biochemistry Source Type: research

Protein kinase Cδ knockout mice are protected from cocaine-induced hepatotoxicity
Publication date: Available online 25 October 2018Source: Chemico-Biological InteractionsAuthor(s): Huynh Nhu Mai, Sung Hoon Lee, Garima Sharma, Dae-Joong Kim, Naveen Sharma, Eun-Joo Shin, Duc Toan Pham, Quynh Dieu Trinh, Choon-Gon Jang, Seung-Yeol Nah, Ji Hoon Jeong, Hyoung-Chun KimAbstractWe investigated whether protein kinase Cδ (PKCδ) mediates cocaine-induced hepatotoxicity in mice. Cocaine treatment (60 mg/kg, i.p.) significantly increased cleaved PKCδ expression in the liver of wild-type (WT) mice, and led to significant increases in oxidative parameters (i.e., reactive oxygen species, 4-hydroxyln...
Source: Chemico Biological Interactions - October 25, 2018 Category: Biochemistry Source Type: research

Long-term diosbulbin B treatment induced liver fibrosis in mice
In conclusion, long-term administration of DB induced liver fibrosis in mice. HSCs activation, epithelial-mesenchymal transition (EMT) and liver inflammation contributed to DB-induced liver fibrosis in mice. (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - October 25, 2018 Category: Biochemistry Source Type: research

Capsaicin is beneficial to hyperlipidemia, oxidative stress, endothelial dysfunction, and atherosclerosis in Guinea pigs fed on a high-fat diet
Publication date: 5 January 2019Source: Chemico-Biological Interactions, Volume 297Author(s): Siyuan Yang, Lin Liu, Like Meng, Xuanyi HuAbstractCapsaicin has anti-inflammatory and antioxidant effects, as well as some benefits on the cardiovascular system. The exact effects of capsaicin on atherosclerosis are poorly understood. To investigate the effects of capsaicin on hyperlipidemia and atherosclerosis in guinea pigs fed on a high-fat diet, as well as its potential mechanisms. Guinea pigs (n = 48) were randomly divided into six groups (n = 8/group): normal diet (control); high fat diet (model); model&n...
Source: Chemico Biological Interactions - October 25, 2018 Category: Biochemistry Source Type: research

Preventive effect of bergenin against the development of TNBS-induced acute colitis in rats is associated with inflammatory mediators inhibition and NLRP3/ASC inflammasome signaling pathways
Conclusion: Our study has provided evidence that administration of bergenin reduced the damage caused by TNBS in an experimental model of acute colitis in rats, reduced levels of pro-inflammatory proteins and cytokines probably by modulation of pSTAT3 and NF-κB signaling and blocking canonical and non-canonical NLRP3/ASC inflammasome pathways.Graphical abstract (Source: Chemico Biological Interactions)
Source: Chemico Biological Interactions - October 24, 2018 Category: Biochemistry Source Type: research

Delphinidin protects β2m−/Thy1+ bone marrow-derived hepatocyte stem cells against TGF-β1-induced oxidative stress and apoptosis through the PI3K/Akt pathway in vitro
Publication date: Available online 23 October 2018Source: Chemico-Biological InteractionsAuthor(s): Jiang Chen, Hong-Yu Li, Di Wang, Xiao-Zhong GuoAbstractβ2m−/Thy1+ bone marrow-derived hepatocyte stem cells (BDHSCs) have a potential to be applied for cellular treatment in liver cirrhosis. However, the resultant tissue regeneration is restricted by transplanted cells' death. The accumulation of transforming growth factor beta 1 (TGF-β1) in liver fibrosis local microenvironment may play an essential role in the rapid cell death of implanted β2m−/Thy1+ BDHSCs. The main mechanism of poor survival of...
Source: Chemico Biological Interactions - October 24, 2018 Category: Biochemistry Source Type: research

Isoquercitrin, a flavonoid glucoside, exerts a positive effect on osteogenesis in vitro and in vivo
Publication date: Available online 23 October 2018Source: Chemico-Biological InteractionsAuthor(s): Jing Li, Xuxia Wang, Yingzi Wang, Chengyan Lu, Dehua Zheng, Jun ZhangAbstractIsoquercitrin (quercetin-3-O-β-d-glucopyranoside) possess various pharmacological effect as a biologically active compound. The aim of the present study was to investigate its potential effects on the proliferation and osteoblastic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) in vitro and bone formation in the mid-palatal suture during rapid maxillary expansion (RME) in vivo. Cell proliferation of rat BMSCs was detected...
Source: Chemico Biological Interactions - October 24, 2018 Category: Biochemistry Source Type: research

Vitexin alleviates streptozotocin-induced sexual dysfunction and fertility impairments in male mice via modulating the hypothalamus–pituitary–gonadal axis
This study investigated the effect of vitexin on male sexual dysfunction and fertility impairments in streptozotocin (STZ)-induced diabetic mice. Diabetes was induced by intraperitoneal injection of 45 mg/kg STZ for 5 consecutive days in mice. Vitexin (10, 20 or 40 mg/kg) and Sildenafil citrate (SC, 5 mg/kg) were administered daily for 62 days after the induction of diabetes. The parameters of sexual behavior and fertility were analyzed. The reproductive organ weight, sperm motility, and viability of the treated mice were examined. Testicular histopathological alterations were detected by hematoxylin and eosin (H&...
Source: Chemico Biological Interactions - October 24, 2018 Category: Biochemistry Source Type: research