β-Lapachone induces ferroptosis of colorectal cancer cells via NCOA4-mediated ferritinophagy by activating JNK pathway
In this study, bulk RNA-seq analysis suggested that β-Lapachone might induce ferroptosis in CRC cells. We further tested this hypothesis using a xenograft model of human colorectal cancer as an animal model and in SW620 and DLD-1 of CRC cell lines. Western blot was used to determine the key proteins of ferroptosis (SLC7A11, GPX4), autophagy (LC3B, P62, ATG7), ferritinophagy (NCOA4, FTH1, TFRC), and JNK pathway (p-JNK, JNK, p-c-Jun, c-Jun). The levels of MDA, GSH/GSSG, lipid ROS, and intracellular ferrous iron were determined after β-Lapachone treatment, and inhibitors of various pathways, including NAC, Ferrostatin-1, DF...
Source: Chemico-Biological Interactions - January 13, 2024 Category: Molecular Biology Authors: Lei Zhao Hui Miao Mingqi Quan Shuhao Wang Yu Zhang Houkun Zhou Xianglan Zhang Zhenhua Lin Junjie Piao Source Type: research

GW4064 inhibits migration and invasion through cathepsin B and MMP2 downregulation in human bladder cancer
In conclusion, GW4064 decreases the migration and invasion of human bladder cancer cells, which may provide a new therapeutic strategy for the treatment of human bladder cancer.PMID:38216027 | DOI:10.1016/j.cbi.2024.110869 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - January 12, 2024 Category: Molecular Biology Authors: Chien-Chang Kao Chien-Rui Lai Yi-Hsuan Lin Tzu-Min Chen Yu-Ling Tsai Wen-Chiuan Tsai Tze-Yun Ong Hisao-Hsien Wang Sheng-Tang Wu Ying Chen Source Type: research

The comprehensive prediction of carcinogenic potency and tumorigenic dose (TD < sub > 50 < /sub > ) for two problematic N-nitrosamines in food: NMAMPA and NMAMBA using toxicology in silico methods
This study aimed to predict the carcinogenic potency and tumorigenic dose (TD50) for two problematic N-nitrosamines (N-NAs) commonly found in food: N-2-methylpropyl-N-1-methylacetonylnitrosamine (NMAMPA, CAS: 93755-83-0) and N-3-Methylbutyl-N-1-methylacetonylnitrosamine (NMAMBA, CAS: 71016-15-4). To achieve this goal, in silico toxicology methods were employed due to their practical applications and potential in risk assessment. The justification for conducting these studies was incoherent results published by the European Food Safety Authority (EFSA). For this purpose, we applied various in silico tools, including qualita...
Source: Chemico-Biological Interactions - January 10, 2024 Category: Molecular Biology Authors: Adrian Frydrych Kamil Jurowski Source Type: research

HNF4A as a potential target of PFOA and PFOS leading to hepatic steatosis: Integrated molecular docking, molecular dynamic and transcriptomic analyses
This study explored the toxicological mechanism of liver steatosis caused by PFOA/PFOS. These compounds might inhibit and down-regulate HNF4A, which is the molecular initiation events (MIE) that induces liver steatosis.PMID:38199259 | DOI:10.1016/j.cbi.2024.110867 (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - January 10, 2024 Category: Molecular Biology Authors: Rui Li Zijing Zhang Yuxin Xuan Yulu Wang Yuyan Zhong Lingyin Zhang Jinrui Zhang Qian Chen Shuling Yu Jintao Yuan Source Type: research

Vitamin D inhibits tamoxifen-induced non-alcoholic fatty liver disease through a nonclassical estrogen receptor/liver X receptor pathway
This study investigated the effects and mechanisms of TAM-induced steatosis in the liver. The effects and mechanisms of TAM on hepatocyte lipid metabolism were assessed using C57BL/6 female mice and human hepatoma cells. TAM promoted fat accumulation in the liver by upregulation of Srebp-1c expression. Regarding the molecular mechanism, TAM promoted the recruitment of the auxiliary transcriptional activator, p300, and dissociated the auxiliary transcriptional repressor, nuclear receptor corepressor (NCOR), of the complexes, which led to enhancement of Srebp-1c transcription and an increase of triglyceride (TG) synthesis. V...
Source: Chemico-Biological Interactions - January 8, 2024 Category: Molecular Biology Authors: Maoxuan Wu Jie Wang Wanqing Zhou Mengting Wang Chunyan Hu Ming Zhou Kailin Jiao Zhong Li Source Type: research

Vitamin D inhibits tamoxifen-induced non-alcoholic fatty liver disease through a nonclassical estrogen receptor/liver X receptor pathway
This study investigated the effects and mechanisms of TAM-induced steatosis in the liver. The effects and mechanisms of TAM on hepatocyte lipid metabolism were assessed using C57BL/6 female mice and human hepatoma cells. TAM promoted fat accumulation in the liver by upregulation of Srebp-1c expression. Regarding the molecular mechanism, TAM promoted the recruitment of the auxiliary transcriptional activator, p300, and dissociated the auxiliary transcriptional repressor, nuclear receptor corepressor (NCOR), of the complexes, which led to enhancement of Srebp-1c transcription and an increase of triglyceride (TG) synthesis. V...
Source: Chemico-Biological Interactions - January 8, 2024 Category: Molecular Biology Authors: Maoxuan Wu Jie Wang Wanqing Zhou Mengting Wang Chunyan Hu Ming Zhou Kailin Jiao Zhong Li Source Type: research

Aldose reductase is a potential therapeutic target for neurodegeneration
Chem Biol Interact. 2024 Jan 5:110856. doi: 10.1016/j.cbi.2024.110856. Online ahead of print.ABSTRACTNeurodegeneration is a complex process involving various inflammatory mediators and cellular responses. Aldose reductase (AR) is a key enzyme in the polyol pathway, which converts glucose to sorbitol. Beyond its metabolic role, AR has also been found to play a significant role in modulating neuroinflammation. This review aims to provide an overview of the current knowledge regarding the involvement of AR inhibition in attenuating neuroinflammation and complications from diabetic neuropathies. Here, we review the literature ...
Source: Chemico-Biological Interactions - January 7, 2024 Category: Molecular Biology Authors: Mishal Rao Kun-Che Chang Source Type: research

Aldose reductase is a potential therapeutic target for neurodegeneration
Chem Biol Interact. 2024 Jan 5:110856. doi: 10.1016/j.cbi.2024.110856. Online ahead of print.ABSTRACTNeurodegeneration is a complex process involving various inflammatory mediators and cellular responses. Aldose reductase (AR) is a key enzyme in the polyol pathway, which converts glucose to sorbitol. Beyond its metabolic role, AR has also been found to play a significant role in modulating neuroinflammation. This review aims to provide an overview of the current knowledge regarding the involvement of AR inhibition in attenuating neuroinflammation and complications from diabetic neuropathies. Here, we review the literature ...
Source: Chemico-Biological Interactions - January 7, 2024 Category: Molecular Biology Authors: Mishal Rao Kun-Che Chang Source Type: research

Cannabinoids improve mitochondrial function in skeletal muscle of exhaustive exercise training rats by inhibiting mitophagy through the Pink1/Parkin and Bnip3 pathways
In this study, by establishing an animal model of exhaustive exercise training in rats, the effects of CBD on the protective effect of CBD on skeletal muscle mitochondrial structure and function was elaborated, and the possible molecular mechanism was discussed based on transcriptomics. Our results indicate that skeletal muscle mitochondrial structure and function were improved after CBD intervention. GO and KEGG pathway enrichment analysis showed that exhaustive exercise training induced mitochondrial dysfunction in skeletal muscle is associated with excessive autophagy/mitophagy, the signaling pathways involved in FOXO3 ...
Source: Chemico-Biological Interactions - January 5, 2024 Category: Molecular Biology Authors: Juncheng Si Lili Sun Ying Qin Lina Peng Yongshan Gong Chun Gao Wenhui Shen Mengqi Li Source Type: research

Cannabinoids improve mitochondrial function in skeletal muscle of exhaustive exercise training rats by inhibiting mitophagy through the Pink1/Parkin and Bnip3 pathways
In this study, by establishing an animal model of exhaustive exercise training in rats, the effects of CBD on the protective effect of CBD on skeletal muscle mitochondrial structure and function was elaborated, and the possible molecular mechanism was discussed based on transcriptomics. Our results indicate that skeletal muscle mitochondrial structure and function were improved after CBD intervention. GO and KEGG pathway enrichment analysis showed that exhaustive exercise training induced mitochondrial dysfunction in skeletal muscle is associated with excessive autophagy/mitophagy, the signaling pathways involved in FOXO3 ...
Source: Chemico-Biological Interactions - January 5, 2024 Category: Molecular Biology Authors: Juncheng Si Lili Sun Ying Qin Lina Peng Yongshan Gong Chun Gao Wenhui Shen Mengqi Li Source Type: research

microRNA-143 targets SIRT2 to mediate the histone acetylation of PLAUR and modulates functions of astrocytes in spinal cord injury
This study aimed to explore effects of microRNA (miR)-143 on the proliferation, apoptosis, and cytokine secretion in astrocytes after spinal cord injury (SCI). After gain- and loss-of-function assays and transforming growth factor (TGF)-β stimulation in astrocytes, the cell viability, proliferation, and apoptosis were examined. The expression of miR-143, SIRT2, and PLAUR and levels of astrocyte-related glial fibrillary acidic protein (GFAP), Vimentin, chondroitin sulfate proteoglycan (CSPG), and connective tissue growth factor (CTGF) were also measured. The binding relationship between miR-143 and SIRT2 was assessed, as w...
Source: Chemico-Biological Interactions - December 31, 2023 Category: Molecular Biology Authors: Changsheng Wang Yi Zhu Rongsheng Chen Xitian Zhu Xiaobo Zhang Source Type: research

microRNA-143 targets SIRT2 to mediate the histone acetylation of PLAUR and modulates functions of astrocytes in spinal cord injury
This study aimed to explore effects of microRNA (miR)-143 on the proliferation, apoptosis, and cytokine secretion in astrocytes after spinal cord injury (SCI). After gain- and loss-of-function assays and transforming growth factor (TGF)-β stimulation in astrocytes, the cell viability, proliferation, and apoptosis were examined. The expression of miR-143, SIRT2, and PLAUR and levels of astrocyte-related glial fibrillary acidic protein (GFAP), Vimentin, chondroitin sulfate proteoglycan (CSPG), and connective tissue growth factor (CTGF) were also measured. The binding relationship between miR-143 and SIRT2 was assessed, as w...
Source: Chemico-Biological Interactions - December 31, 2023 Category: Molecular Biology Authors: Changsheng Wang Yi Zhu Rongsheng Chen Xitian Zhu Xiaobo Zhang Source Type: research

microRNA-143 targets SIRT2 to mediate the histone acetylation of PLAUR and modulates functions of astrocytes in spinal cord injury
This study aimed to explore effects of microRNA (miR)-143 on the proliferation, apoptosis, and cytokine secretion in astrocytes after spinal cord injury (SCI). After gain- and loss-of-function assays and transforming growth factor (TGF)-β stimulation in astrocytes, the cell viability, proliferation, and apoptosis were examined. The expression of miR-143, SIRT2, and PLAUR and levels of astrocyte-related glial fibrillary acidic protein (GFAP), Vimentin, chondroitin sulfate proteoglycan (CSPG), and connective tissue growth factor (CTGF) were also measured. The binding relationship between miR-143 and SIRT2 was assessed, as w...
Source: Chemico-Biological Interactions - December 31, 2023 Category: Molecular Biology Authors: Changsheng Wang Yi Zhu Rongsheng Chen Xitian Zhu Xiaobo Zhang Source Type: research

Solvent isotope and mutagenesis studies on the proton relay system in yeast alcohol dehydrogenase 1
Chem Biol Interact. 2023 Dec 25:110853. doi: 10.1016/j.cbi.2023.110853. Online ahead of print.ABSTRACTAlcohol dehydrogenase catalyzes the reversible transfer of a hydride directly from an alcohol to the nicotinamide ring of NAD+ to form an aldehyde and NADH, and the proton from the alcohol probably is transferred through a hydrogen-bonded system to the imidazole of His-48. Studies of the pH dependencies, and solvent and substrate isotope effects on the wild-type and the enzyme with His-48 substituted with Gln-48 were used to demonstrate a role for the proton relay system. The H48Q substitution increases affinities for NAD+...
Source: Chemico-Biological Interactions - December 27, 2023 Category: Molecular Biology Authors: Bryce V Plapp Source Type: research

Solvent isotope and mutagenesis studies on the proton relay system in yeast alcohol dehydrogenase 1
Chem Biol Interact. 2023 Dec 25:110853. doi: 10.1016/j.cbi.2023.110853. Online ahead of print.ABSTRACTAlcohol dehydrogenase catalyzes the reversible transfer of a hydride directly from an alcohol to the nicotinamide ring of NAD+ to form an aldehyde and NADH, and the proton from the alcohol probably is transferred through a hydrogen-bonded system to the imidazole of His-48. Studies of the pH dependencies, and solvent and substrate isotope effects on the wild-type and the enzyme with His-48 substituted with Gln-48 were used to demonstrate a role for the proton relay system. The H48Q substitution increases affinities for NAD+...
Source: Chemico-Biological Interactions - December 27, 2023 Category: Molecular Biology Authors: Bryce V Plapp Source Type: research