Atorvastatin combined with imipenem alleviates lung injury in sepsis by inhibiting neutrophil extracellular trap formation via the ERK/NOX2 signaling pathway
In this study, we used a cecum ligation and puncture-induced lung injury mouse model and employed techniques including western blot, immunofluorescence, and enzyme-linked immunosorbent assay to measure the levels of NETs and other sepsis-related lung injury indicators. Our findings indicate that atorvastatin effectively inhibited the formation of NETs. When combined with imipenem, it significantly alleviated lung injury, reduced systemic inflammation, and improved the 7-day survival rate of septic mice. Additionally, we explored the inhibitory mechanism of atorvastatin on NET formation in vitro, revealing its potential act...
Source: Free Radical Biology and Medicine - May 4, 2024 Category: Biology Authors: Yue Zhang Di Wu Qishun Sun Zhen Luo Yuhao Zhang Bowei Wang Wenting Chen Source Type: research
Maternal selenium deficiency during pregnancy in association with autism and ADHD traits in children: the Odense Child Cohort
CONCLUSIONS: The results provide coherent evidence for selenium deficiency as a risk factor for ADHD and ASD traits in an environment with borderline supply, the causality of which should be elucidated in a randomized controlled trial.PMID:38704054 | DOI:10.1016/j.freeradbiomed.2024.05.001 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - May 4, 2024 Category: Biology Authors: Kamil Demircan Thilo Samson Chillon Richard Christian Jensen Tina Kold Jensen Qian Sun Steen Joop Bonnema Dorte Glintborg Niels Bilenberg Marianne Skovsager Andersen Lutz Schomburg Source Type: research
Metallofullerenol alleviates alcoholic liver damage via ROS clearance under static magnetic and electric fields
Free Radic Biol Med. 2024 May 2:S0891-5849(24)00431-3. doi: 10.1016/j.freeradbiomed.2024.05.003. Online ahead of print.ABSTRACTAlcoholic liver disease (ALD) is a common chronic redox disease caused by increased alcohol consumption. Abstinence is a major challenge for people with alcohol dependence, and approved drugs have limited efficacy. Therefore, this study aimed to explore a new treatment strategy for ALD using ferroferric oxide endohedral fullerenol (Fe3O4@C60(OH)n) in combination with static magnetic and electric fields (sBE). The primary hepatocytes of 8-9-week-old female BALB/c mice were used to evaluate the effic...
Source: Free Radical Biology and Medicine - May 4, 2024 Category: Biology Authors: Haoyu Wang Junqi Zhao Shiliang Ji Tingjun Liu Zhisheng Chen Zhen Huang Yuhui Zang Jiangning Chen Junfeng Zhang Zhi Ding Source Type: research
Ketogenesis attenuated KLF5 disrupts iron homeostasis via LIF to confer oxaliplatin vulnerability in colorectal cancer
This study aimed to determine whether the consumption of a KD increases the sensitivity of CRC cells to oxaliplatin via the inhibition of a classical stem cell marker, Krupple-like factor 5 (KLF5). KLF5 functions as a transcription factor for the leukemia inhibitory factor (LIF) and directly binds to its promoter region. LIF upregulation induces dephosphorylation of metal regulatory transcription factor 1 (MTF1), which is recruited to the promoter area of Ferroportin (FPN1), the only cellular iron exporter. FPN1 upregulation reduces the labile iron pool (LIP) and ferroptosis in CRC cells. KLF5 knockdown inhibits the LIF/MT...
Source: Biochimica et Biophysica Acta - May 4, 2024 Category: Biochemistry Authors: Haoran Jiang Yuan Zeng Xue Jiang Xuni Xu Lihao Zhao Xiaoye Yuan Jun Xu Mengjing Zhao Fang Wu Gang Li Source Type: research
A complete biosynthetic pathway of the long-chain polyunsaturated fatty acids in an amphidromous fish, ayu sweetfish Plecoglossus altivelis (Stomiati; Osmeriformes)
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 May 3;1869(6):159498. doi: 10.1016/j.bbalip.2024.159498. Online ahead of print.ABSTRACTThe biosynthetic capability of the long-chain polyunsaturated fatty acids (LC-PUFA) in teleosts are highly diversified due to evolutionary events such as gene loss and subsequent neo- and/or sub-functionalisation of enzymes encoded by existing genes. In the present study, we have comprehensively characterised genes potentially involved in LC-PUFA biosynthesis, namely one front-end desaturase (fads2) and eight fatty acid elongases (elovl1a, elovl1b, elovl4a, elovl4b, elovl5, elovl7, elovl8a ...
Source: Biochimica et Biophysica Acta - May 4, 2024 Category: Biochemistry Authors: Bo Zhao Yingying Peng Yuki Itakura Myriam Lizanda Yutaka Haga Shuichi Satoh Juan C Navarro Óscar Monroig Naoki Kabeya Source Type: research
Deorphanizing an odorant receptor tuned to palm tree volatile esters in the Asian palm weevil sheds light on the mechanisms of palm tree selection
This study identified a highly expressed antennal R. ferrugineus OR, RferOR2, thanks to newly generated transcriptomic data. The phylogenetic analysis revealed that RferOR2 belongs to the major coleopteran OR group 2A and is closely related to a sister clade containing an R. ferrugineus OR (RferOR41) tuned to the non-host plant volatile and antagonist, α-pinene. Functional characterization of RferOR2 via heterologous expression in Drosophila olfactory neurons revealed that this receptor is tuned to several ecologically relevant palm-emitted odors, most notably ethyl and methyl ester compounds, but not to any of the pherom...
Source: Insect Biochemistry and Molecular Biology - May 4, 2024 Category: Biochemistry Authors: Binu Antony Nicolas Montagn é Arthur Comte Sara Mfarrej Jernej Jak še R émi Capoduro Rajan Shelke Khasim Cali Mohammed Ali AlSaleh Krishna Persaud Arnab Pain Emmanuelle Jacquin-Joly Source Type: research
Bottom-up Growth of Convex Sphere with Adjustable Cu(0)/Cu(I) Interfaces for Effective C2 Production from CO2 Electroreduction
In this study, we for the first time introduce a bottom-up growth of convex sphere with adjustable Cu(0)/Cu(I) interfaces (Cux@Cu2O convex spheres). Interestingly, the interfaces are dynamically modulated by varying hydrothermal time, thus regulating the conversion of C1 and C2 products. In particular, the 4 h hydrothermal treatment applied to Cu0.25@Cu2O convex sphere with the favorable Cu(0)/Cu(I) interface results in the highest selectivity for C2 products (90.5%). In situ Fourier-transform infrared spectroscopy measurements and density functional theory calculations reveal that the Cu(0)/Cu(I) interface lowers the ener...
Source: Angewandte Chemie - May 4, 2024 Category: Chemistry Authors: Huan Liu Chenghan Yang Tong Bian Huijun Yu Yuming Zhou Yiwei Zhang Source Type: research
The effect of ultrasonic power on the physicochemical properties and antioxidant activities of frosted figs pectin
This study optimized the ultrasonic-assisted extraction (UAE) process of frosted figs pectin (FFP) using response surface methodology (RSM), and further investigated the effect of ultrasonic power on the structural characteristics and antioxidant activities of FFPs. The UAE method of FFP through RSM was optimized, and the optimal extraction process conditions, particle size of 100 mesh, pH value of 1.95, liquid-solid ratio of 47:1 (mL/g), extraction temperature of 50 °C and extraction time of 65 min, were obtained. The extraction rate of FFP under this condition was 37.97 ± 2.56 %. Then, the four FFPs modified by ultraso...
Source: Ultrasonics Sonochemistry - May 4, 2024 Category: Chemistry Authors: Rui-Xue Deng Yi-Ying Zheng Dong-Jie Liu Jing-Yi Liu Meng-Nan Zhang Guang-Yuan Xi Lu-Lu Song Pu Liu Source Type: research
New insight into molecular mechanisms of different polyphenols affecting Sirtuin 3 deacetylation activity
This study constructed a variety of reaction systems to elucidate the mechanisms by which different polyphenols affect SIRT3 enzyme activity. The results showed that acP53317-320 was the most suitable substrate among the five acetylated peptide substrates (Kcat/Km = 74.85 ± 1.86 M-1•s-1). All the phenolic compounds involved in the experiment inhibited the enzymatic activity of SIRT3, and the lowest IC50 among them was quercetin (0.12 ± 0.01 mM) and the highest was piceatannol (1.29 ± 0.08 mM). Their inhibition types were mainly competitive and mixed. In addition, piceatannol was found to be a natural SIRT3 agonist by ...
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Yulong Wei Yiman Zhang Ziyuan Wang Zihui Yang Zongwei Wang Yiming Hao Genying Li Fei Gao Gaoqi Ye Jing Wang Jie Liu Source Type: research
Niobium-based single-atom catalyst promoted fractionation of lignocellulose in choline chloride-lactic acid deep eutectic solvent
In this study, a green pretreatment method based on choline chloride-lactic acid deep eutectic solvent (ChCl-LA) and niobium-based single-atom catalyst (Nb/CN) was developed for the fractionation of corn straw and further enzymatic hydrolysis of cellulose. With this strategy, significant lignin removal of 96.5 % could be achieved when corn straw was pretreated by ChCl-LA (1:2) DES over Nb/CN under 120 °C for 6 h. Enzymatic hydrolysis of the cellulose-enriched fraction (CEF) presented high glucose yield of 92.7 % and xylose yield of 67.5 %. In-depth investigations verified that the high yields of fractions and monosacchari...
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Zhen Wu Tao Wang Zihe Zhao Yifan Ji Hongli Bai Yetao Jiang Xiaoyu Wang Haq Nawaz Aiyong He Jun Xia Jiaxing Xu Sheng Chen Lei Hu Source Type: research
Discovery of a novel chemotype as DYRK1A inhibitors against Alzheimer's disease: Computational modeling and biological evaluation
Int J Biol Macromol. 2024 May 2:132024. doi: 10.1016/j.ijbiomac.2024.132024. Online ahead of print.ABSTRACTDual-specificity tyrosine phosphorylation-regulated kinase 1 A (DYRK1A) plays an essential role in Tau and Aβ pathology closely related to Alzheimer's disease (AD). Accumulative evidence has demonstrated DYRK1A inhibition is able to reduce the pathological features of AD. Nevertheless, there is no approved DYRK1A inhibitor for clinical use as anti-AD therapy. This is somewhat due to the lack of effective and safe chemotypes of DYRK1A inhibitors. To address this issue, we carried out in silico screening, in vitro assa...
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Nianzhuang Qiu Chenliang Qian Tingting Guo Yaling Wang Hongwei Jin Mingli Yao Mei Li Tianyang Guo Yuli Lv Xinxin Si Song Wu Hao Wang Xuehui Zhang Jie Xia Source Type: research
A review on structure-function mechanism and signaling pathway of serine/threonine protein PIM kinases as a therapeutic target
In conclusion, we provide insights into the benefits of degrading PIM kinases as opposed to blocking their catalytic activity to address the oncogenic potential of PIM kinases.PMID:38704069 | DOI:10.1016/j.ijbiomac.2024.132030 (Source: International Journal of Biological Macromolecules)
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Ajaya Kumar Rout Budheswar Dehury Satya Narayan Parida Sushree Swati Rout Rajkumar Jena Neha Kaushik Nagendra Kumar Kaushik Sukanta Kumar Pradhan Chita Ranjan Sahoo Ashok Kumar Singh Meenakshi Arya Bijay Kumar Behera Source Type: research
Fractionation of cassava pectins and their detailed structural analyses using various pectinolytic enzymes
In this study, we analyzed the pectin structure within the pulp of cassava. Cassava pectin, derived from cassava pulp treatment at 120 °C for 90 min, was separated into four fractions (CP-P, CP-SD1, CP-SD2F, and CP-SD2R) based on variations in water solubility, electrical properties, and molecular weights. Sugar composition analysis demonstrated an abundance of homogalacturonan (HG) in CP-P and CP-SD2F, rhamnogalacturonan I (RG-I) in CP-SD2R, and neutral sugars in CP-SD1. Because RG-I possesses a complex structure, we analyzed CP-SD2R using various pectinolytic enzymes. Galactose was the major sugar in CP-SD2R accounting ...
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Yuka Kotani Nozomu Shibata Meng-I Lin Masami Nakazawa Mitsuhiro Ueda Tatsuji Sakamoto Source Type: research
CXCR2, as a key regulatory gene of HDP-PG-1, maintains intestinal mucosal homeostasis
Int J Biol Macromol. 2024 May 2:132025. doi: 10.1016/j.ijbiomac.2024.132025. Online ahead of print.ABSTRACTThe intestine defends against pathogenic microbial invasion via the secretion of host defense peptides (HDPs). Nutritional immunomodulation can stimulate the expression of endogenous HDPs and enhance the body's immune defense, representing a novel non-antibiotic strategy for disease prevention. The project aims to explore the regulatory mechanism of protegrin-1 (PG-1) expression using sodium phenylbutyrate (PBA) by omics sequencing technology and further investigate the role of key regulatory genes on intestinal healt...
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Di Yan Guoyang Wei Zichun Ai Shuang Song Licong Zhang Na Dong Xiujing Dou Anshan Shan Source Type: research
Inhibition of protein misfolding and aggregation by steroidal quinoxalin-2(1H)-one and their molecular docking studies
Int J Biol Macromol. 2024 May 2:132020. doi: 10.1016/j.ijbiomac.2024.132020. Online ahead of print.ABSTRACTA series of D-ring fused 16-substituted steroidal quinoxalin-2(1H)-one attached to an electron-releasing (ER) or electron-withdrawing (EW) groups via steroidal oxoacetate intermediate were synthesized to investigate their protein aggregation inhibition potential using human lysozyme (HLZ). The influence of the type of substituent at the C-6 positions of the quinoxalin-2(1H)-one ring on the protein aggregation inhibition potential was observed, showing that the EW moiety improved the protein aggregation inhibition pote...
Source: International Journal of Biological Macromolecules - May 4, 2024 Category: Biochemistry Authors: Arfeen Iqbal Md Tauqir Alam Asna Khan Tabassum Siddiqui Abad Ali Source Type: research
