TET1 mediated m5C modification of RelB aggravates cerebral ischemia/reperfusion-induced neuroinflammation through regulating microglia polarization
In conclusion, this study provides insight into the role of m5C RNA modification in the pathogenesis of cerebral I/R neuroinflammation and may deepen our understanding on clinical therapy targeting the TET1-RelB axis.PMID:38705503 | DOI:10.1016/j.cellsig.2024.111210 (Source: Cellular Signalling)
Source: Cellular Signalling - May 5, 2024 Category: Cytology Authors: Yan Lin Mei Liu Pinghuan Deng Jinzhi Zhang Source Type: research
USP10 alleviates N ε-carboxymethyl-lysine-induced vascular calcification and atherogenesis in diabetes mellitus by promoting AMPK activation
This study aims to elucidate the role of USP10 in VC development and the underlying regulatory mechanisms. Nε-carboxymethyl lysine (CML) was significantly increased in calcified ateries from diabetic atherosclerosis ApoE-/- mice fed with high-fat diets. CML downregulated USP10 expression in VSMCs and calcified mice coronary arteries, as assessd by Western blotting, RT-qPCR,immunofluorescence and immunohistochemistry. Loss-and gain-of-function experiments were conducted both in vitro and in vivo to verify the biological functions of USP10. Ectopic expression of USP10 mitigated the severity of VC. With regard to the mechani...
Source: Cellular Signalling - May 5, 2024 Category: Cytology Authors: Mayibai Mushajiang Li Yalan Sun Zhen Liu Jia Zhang Lili Wang Zhongqun Source Type: research
The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function
In conclusion, our findings provide initial insights into the regulatory role of the mammalian actin elongation factor ENAH in autophagy.PMID:38705725 | DOI:10.1080/15548627.2024.2347105 (Source: Autophagy)
Source: Autophagy - May 5, 2024 Category: Cytology Authors: Yueheng Li Yafei Zhang Menghui Wang Junhui Su Xinjue Dong Yuqi Yang Hongshan Wang QingQuan Li Source Type: research
USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy
In this study, we screened deubiquitinating enzymes (DUBs) involved in reticulophagy and identified USP20 (ubiquitin specific peptidase 20) as a key regulator of reticulophagy under starvation conditions. USP20 specifically cleaves K48- and K63-linked ubiquitin chains on the reticulophagy receptor RETREG1/FAM134B (reticulophagy regulator 1), thereby stabilizing the substrate and promoting reticulophagy. Remarkably, despite lacking a transmembrane domain, USP20 is recruited to the ER through its interaction with VAPs (VAMP associated proteins). VAPs facilitate the recruitment of early autophagy proteins, including WIPI2 (WD...
Source: Autophagy - May 5, 2024 Category: Cytology Authors: Man Zhang Zhangshun Wang Qing Zhao Qian Yang Jieyun Bai Cuiwei Yang Zai-Rong Zhang Yanfen Liu Source Type: research
Post-error adjustments occur in both reaching and grasping
Exp Brain Res. 2024 May 5. doi: 10.1007/s00221-024-06836-5. Online ahead of print.ABSTRACTPost-error slowing (PES), the tendency to slow down a behavioral response after a previous error, has typically been investigated during simple cognitive tasks using response time as a measure of PES magnitude. More recently, PES was investigated during a single reach-to-grasp task to determine where post-error adjustments are employed in a more ecological setting. Kinematic analyses in the previous study detected PES during pre-movement planning and within the grasping component of movement execution. In the current study (N = 22), w...
Source: Experimental Brain Research - May 5, 2024 Category: Neuroscience Authors: Joe Opdenaker Jarrod Blinch Miranda Scolari Source Type: research
Quantitative profiling of cochlear synaptosomal proteins in cisplatin-induced synaptic dysfunction
This study tests the hypothesis that cisplatin treatment alters the abundance of cochlear synaptosomal proteins, and selective targeting of nitrative stress prevents the associated synaptic dysfunction. Auditory brainstem responses of mice treated with cisplatin showed a reduction in amplitude and an increase in latency of wave I, indicating cisplatin-induced synaptic dysfunction. The mass spectrometry analysis of cochlear synaptosomal proteins identified 102 proteins that decreased in abundance and 249 that increased in abundance after cisplatin treatment. Pathway analysis suggested that the dysregulated proteins were inv...
Source: Hearing Research - May 5, 2024 Category: Audiology Authors: Monazza Shahab Rita Rosati Paul M Stemmer Alan Dombkowski Samson Jamesdaniel Source Type: research
Novel Pyrazole-Chalcone Hybrids: Synthesis and Computational Insights Against Breast Cancer
Chem Biodivers. 2024 May 5:e202400015. doi: 10.1002/cbdv.202400015. Online ahead of print.ABSTRACTMore women die of breast cancer than of any other malignancy. The resistance and toxicity of traditional hormone therapy created an urgent need for potential molecules for treating breast cancer effectively. Novel biphenyl-substituted pyrazole chalcones linked to a pyrrolidine ring were designed by using a hybridization approach. The hybrids were assessed against MCF-7 and MDA-MB-231 cells by NRU assay. Among them, 8k, 8d, 8m, 8h, and 8f showed significantly potent IC50 values: 0.17, 5.48, 8.13, 20.51, and 23.61 µM) respectiv...
Source: Chemistry and Biodiversity - May 5, 2024 Category: Biochemistry Authors: Pratap S Dabhade Manjushri P Dabhade Lala S Rathod Sachin A Dhawale Shweta A More Somdatta Y Chaudhari Santosh N Mokale Source Type: research
Structural Characterization and Screening for Anti-inflammatory Activity of Polysaccharides with Different Molecular Weights from Astragali Radix
In this study, lipopolysaccharide (LPS)-induced RAW264.7 macrophages were used as a model to investigate the anti-inflammatory activity of APSs and its fractions. The results revealed that fraction APS-I had better anti-inflammatory effects than APS-II. After APS-Ⅰ was hydrolyzed by trifluoroacetic acid (TFA), the resulting degradation products oligosaccharides were fully methylated. These derivatized oligosaccharides were further analyzed by MALDI-TOF-MS and UPLC-Q-Exactive-MS/MS. The results showed that APS-Ⅰ was a hetero-polysaccharide with a molecular weight of about 2.0 × 106 Da, mainly consisting of glucose (46....
Source: Chemistry and Biodiversity - May 5, 2024 Category: Biochemistry Authors: Xinhui Fan Ke Li Xuemei Qin Zhenyu Li Yuguang Du Source Type: research
The social and organisational factors shaping acceptability of a self-management education and exercise intervention for people with hip or knee osteoarthritis in Greenland
This study aimed to explore the experiences and perspectives of people with osteoarthritis attending the "Osteoarthritis School" (OA School) in Nuuk, Greenland to generate insights and lessons that can inform the development of self-management education and exercise interventions for people with other lifestyle conditions in a Greenland context. We conducted a qualitative interpretive description (ID) study based on ten semi-structured interviews with people with hip or knee osteoarthritis. Interviews were audio-recorded, transcribed, and coded. Using ID, we identified three themes: 1) perceptions and experiences of how th...
Source: International Journal of Circumpolar Health - May 5, 2024 Category: International Medicine & Public Health Authors: Marie Tolver Nielsen Maja Hykkelbjerg Nielsen Sonja S ørensen Morten Skovdal Source Type: research
Understanding the Mechanochemistry of Mechano-Radicals in Self-Growth Materials by Single-Molecule Force Spectroscopy
In this study, we investigated the mechanical strength of three azo compounds using single-molecule force spectroscopy. Our results revealed that these compounds exhibit rupture forces ranging from ~500 to 1000 pN, at a loading rate of 3×104 pN s-1. Importantly, these mechanophores demonstrate distinct kinetic properties. Their unique mechanical attributes enable azo bond scission and free radical generation before causing major polymer backbone damage of entire material during polymer network deformation. This fundamental understanding of mechanophores holds significant promise for the development of self-growth material...
Source: Chemphyschem - May 5, 2024 Category: Chemistry Authors: Jing Liu Jiahui Yang Bin Xue Yi Cao Wei Cheng Yiran Li Source Type: research
NLRP3-dependent pyroptosis exacerbates Coxsackievirus A16 and Coxsackievirus A10-induced inflammatory response and viral replication in SH-SY5Y cells
Virus Res. 2024 May 3:199386. doi: 10.1016/j.virusres.2024.199386. Online ahead of print.ABSTRACTCoxsackievirus A16 (CV-A16) and Coxsackievirus A10 (CV-A10), more commonly etiological agents of hand, foot and mouth disease (HFMD), are capable of causing severe neurological syndromes with high fatalities, but their neuropathogenesis has rarely been studied. Mounting evidence indicated that pyroptosis is an inflammatory form of cell death that might be widely involved in the pathogenic mechanisms of neurotropic viruses. Our study was designed to examine the effects of NLRP3-mediated pyroptosis in CV-A16- and CV-A10-induced i...
Source: Virus Research - May 5, 2024 Category: Virology Authors: Yajie Hu Wei Zhao Yaming Lv Hui Li Jiang Li Mingmei Zhong Dandan Pu Fuping Jian Jie Song Yunhui Zhang Source Type: research
Adverse childhood experiences, gender, and suicidality among Florida high school students: Examining intervening mechanisms
CONCLUSIONS: For male and female youth, ACEs are associated with heightened depression symptoms and reduced self-control, and both of these factors partially explain the previously established relationship between ACEs and suicidality.PMID:38704885 | DOI:10.1016/j.chiabu.2024.106834 (Source: Child Abuse and Neglect)
Source: Child Abuse and Neglect - May 5, 2024 Category: Child Development Authors: Melissa S Jones Salpi S Kevorkian Peter S Lehmann Cecilia Chouhy Ryan C Meldrum Source Type: research
Co-resistance to methicillin and clindamycin among coagulase-negative staphylococci isolates recovered from pet food in Brazil
Int J Food Microbiol. 2024 Apr 25;418:110726. doi: 10.1016/j.ijfoodmicro.2024.110726. Online ahead of print.ABSTRACTPet food have been considered as possible vehicles of bacterial pathogens. The sudden boom of the pet food industry due to the worldwide increase in companion animal ownership calls for pet food investigations. Herein, this study aimed to determine the frequency, antimicrobial susceptibility profile, and molecular characteristics of coagulase-negative staphylococci (CoNS) in different pet food brands in Brazil. Eighty-six pet food packages were screened for CoNS. All isolates were identified at species level ...
Source: International Journal of Food Microbiology - May 5, 2024 Category: Food Science Authors: Ikechukwu Benjamin Moses Ingrid Nayara Marcelino Santos Ághata Cardoso da Silva Ribeiro Fernanda Fernandes Santos Rodrigo Cay ô Ana Cristina Gales Source Type: research
Insecure attachment and support-seeking during COVID-19: a sequential mixed methods investigation
Attach Hum Dev. 2024 May 4:1-22. doi: 10.1080/14616734.2024.2349577. Online ahead of print.ABSTRACTThe COVID-19 pandemic led many couples to stay at home together with minimal contact with others. As social distancing measures reduced contact with outside households, many partners could seek support only from one another. In two studies using a sequential mixed methods design, we investigated support-seeking behaviors in romantic relationships during COVID-19. In the qualitative study, semi-structured interviews (n = 48) showed differences in how and why partners seek support with an initial consideration of the role of at...
Source: Attachment and Human Development - May 5, 2024 Category: Child Development Authors: Rachel R R Francois-Walcott Rhia E Perks Laura M Vowels Katherine B Carnelley Source Type: research
GEHGAN: CircRNA-disease association prediction via graph embedding and heterogeneous graph attention network
Comput Biol Chem. 2024 Apr 25;110:108079. doi: 10.1016/j.compbiolchem.2024.108079. Online ahead of print.ABSTRACTThere is growing proof suggested that circRNAs play a crucial function in diverse important biological reactions related to human diseases. Within the area of biochemistry, a massive range of wet experiments have been carried out to find out the connections of circRNA-disease in recent years. Since wet experiments are expensive and laborious, nowadays, calculation-based solutions have increasingly attracted the attention of researchers. However, the performance of these methods is restricted due to the inability...
Source: Computational Biology and Chemistry - May 5, 2024 Category: Bioinformatics Authors: Yuehao Wang Pengli Lu Source Type: research
