In vitro and in vivo antiviral effects of CLEVir-X against porcine reproductive and respiratory syndrome virus
Virus Res. 2024 Apr 22:199380. doi: 10.1016/j.virusres.2024.199380. Online ahead of print.ABSTRACTThe aim of this study was to investigate the in vitro and in vivo antiviral effects of CLEVir-X, against porcine reproductive and respiratory syndrome virus (PRRSV). CLEVir-X is a nucleoside analogue and a dialdehyde form of xanthosine. CLEVir-X demonstrated antiviral action during the in vitro portion of this experiment with its inosine monophosphate dehydrogenase (IMPDH) inhibition against PRRSV. The anti-PRRSV effect of CLEVir-X was recovered through supplementation with guanosine. This suggests that PRRSV replication may b...
Source: Virus Research - April 24, 2024 Category: Virology Authors: Jeongmin Suh Sehyeong Ham Youngnam Kim Sunghun Kim Ahreum Cho Hojin Moon Chanhee Chae Source Type: research
Japanese encephalitis virus E protein domain III immunization mediates cross-protection against Zika virus in mice via antibodies and CD8 < sup > + < /sup > T cells
Virus Res. 2024 Apr 19:199376. doi: 10.1016/j.virusres.2024.199376. Online ahead of print.ABSTRACTZika virus (ZIKV) and Japanese encephalitis virus (JEV) are antigenically related flaviviruses that co-circulate in many countries/territories. The interaction between the two viruses needs to be determined. Recent findings by ourselves and other labs showed that JEV-elicited antibodies (Abs) and CD8+ T cells exacerbate and protect against subsequent ZIKV infection, respectively. However, the impact of JEV envelope (E) protein domain III (EDIII)-induced immune responses on ZIKV infection is unclear. We show here that sera from...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Zhi-Liang Duan Wei-Wei Zou Dong Chen Jia-Yang Zhu Jin-Sheng Wen Source Type: research
A live attenuated influenza B virus vaccine expressing RBD elicits protective immunity against SARS-CoV-2 in mice
In this study, we constructed an IBV-based vector vaccine by inserting a receptor-binding domain (RBD) into a non-structural protein 1 (NS1)-truncated gene (rIBV-NS110-RBD). Subsequently, we assessed its safety, immunogenicity, and protective efficacy against SARS-CoV-2 in mice, and observed that it was safe in a mouse model. Intranasal administration of a recombinant rIBV-NS110-RBD vaccine induced high levels of SARS-CoV-2-specific IgA and IgG antibodies and T cell-mediated immunity in mice. Administering two doses of the intranasal rIBV-NS110-RBD vaccine significantly reduced the viral load and lung damage in mice. This ...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Zhenfei Wang Weiyang Sun Dongxu Li Yue Sun Menghan Zhu Wenqi Wang Yiming Zhang Entao Li Feihu Yan Tiecheng Wang Na Feng Songtao Yang Xianzhu Xia Yuwei Gao Source Type: research
Amino Acid Substitution of the Membrane-Proximal External Region Alter Neutralization Sensitivity in a Chronic HIV-1 Clade B Infected Patient
In this study, we employed single genome amplification to amplify 34 full-length env sequences from the 2005 plasma sample of CBJC504, a chronic HIV-1 clade B infected individual. We identified three amino acid changes (N671S, D674N, and K677R) in the MPER. A longitudinal analysis revealed that the proportion of env sequences with MPER mutations increased from 26.5% in 2005 to 56.0% in 2009, and the sequences with the same mutation clustered together. Nine functional pseudoviruses were generated from the 34 env sequences to examine the effect of these mutations on neutralizing activity. Pseudoviruses carrying N674 or R677 ...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Yuyu Fu Shuhui Wang Yanling Hao Dan Li Li Ren Zheng Wang Ran Chen Wenqi Tang Xiuli Shen Wanqi Ni Yutao Shi Meiling Zhu Yiming Shao Ying Liu Source Type: research
The varicella-zoster virus ORF16 protein promotes both the nuclear transport and the protein abundance of the viral DNA polymerase subunit ORF28
Virus Res. 2024 Apr 19:199379. doi: 10.1016/j.virusres.2024.199379. Online ahead of print.ABSTRACTAlthough all herpesviruses utilize a highly conserved replication machinery to amplify their viral genomes, different members may have unique strategies to modulate the assembly of their replication components. Herein, we characterize the subcellular localization of seven essential replication proteins of varicella-zoster virus (VZV) and show that several viral replication enzymes such as the DNA polymerase subunit ORF28, when expressed alone, are localized in the cytoplasm. The nuclear import of ORF28 can be mediated by the v...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Huang-Shen Lin Cheng-Han Li Lee-Wen Chen Shie- Shan Wang Li- Yu Chen Chien-Hui Hung Chun-Liang Lin Pey-Jium Chang Source Type: research
Japanese encephalitis virus E protein domain III immunization mediates cross-protection against Zika virus in mice via antibodies and CD8 < sup > + < /sup > T cells
Virus Res. 2024 Apr 19:199376. doi: 10.1016/j.virusres.2024.199376. Online ahead of print.ABSTRACTZika virus (ZIKV) and Japanese encephalitis virus (JEV) are antigenically related flaviviruses that co-circulate in many countries/territories. The interaction between the two viruses needs to be determined. Recent findings by ourselves and other labs showed that JEV-elicited antibodies (Abs) and CD8+ T cells exacerbate and protect against subsequent ZIKV infection, respectively. However, the impact of JEV envelope (E) protein domain III (EDIII)-induced immune responses on ZIKV infection is unclear. We show here that sera from...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Zhi-Liang Duan Wei-Wei Zou Dong Chen Jia-Yang Zhu Jin-Sheng Wen Source Type: research
A live attenuated influenza B virus vaccine expressing RBD elicits protective immunity against SARS-CoV-2 in mice
In this study, we constructed an IBV-based vector vaccine by inserting a receptor-binding domain (RBD) into a non-structural protein 1 (NS1)-truncated gene (rIBV-NS110-RBD). Subsequently, we assessed its safety, immunogenicity, and protective efficacy against SARS-CoV-2 in mice, and observed that it was safe in a mouse model. Intranasal administration of a recombinant rIBV-NS110-RBD vaccine induced high levels of SARS-CoV-2-specific IgA and IgG antibodies and T cell-mediated immunity in mice. Administering two doses of the intranasal rIBV-NS110-RBD vaccine significantly reduced the viral load and lung damage in mice. This ...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Zhenfei Wang Weiyang Sun Dongxu Li Yue Sun Menghan Zhu Wenqi Wang Yiming Zhang Entao Li Feihu Yan Tiecheng Wang Na Feng Songtao Yang Xianzhu Xia Yuwei Gao Source Type: research
Amino Acid Substitution of the Membrane-Proximal External Region Alter Neutralization Sensitivity in a Chronic HIV-1 Clade B Infected Patient
In this study, we employed single genome amplification to amplify 34 full-length env sequences from the 2005 plasma sample of CBJC504, a chronic HIV-1 clade B infected individual. We identified three amino acid changes (N671S, D674N, and K677R) in the MPER. A longitudinal analysis revealed that the proportion of env sequences with MPER mutations increased from 26.5% in 2005 to 56.0% in 2009, and the sequences with the same mutation clustered together. Nine functional pseudoviruses were generated from the 34 env sequences to examine the effect of these mutations on neutralizing activity. Pseudoviruses carrying N674 or R677 ...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Yuyu Fu Shuhui Wang Yanling Hao Dan Li Li Ren Zheng Wang Ran Chen Wenqi Tang Xiuli Shen Wanqi Ni Yutao Shi Meiling Zhu Yiming Shao Ying Liu Source Type: research
The varicella-zoster virus ORF16 protein promotes both the nuclear transport and the protein abundance of the viral DNA polymerase subunit ORF28
Virus Res. 2024 Apr 19:199379. doi: 10.1016/j.virusres.2024.199379. Online ahead of print.ABSTRACTAlthough all herpesviruses utilize a highly conserved replication machinery to amplify their viral genomes, different members may have unique strategies to modulate the assembly of their replication components. Herein, we characterize the subcellular localization of seven essential replication proteins of varicella-zoster virus (VZV) and show that several viral replication enzymes such as the DNA polymerase subunit ORF28, when expressed alone, are localized in the cytoplasm. The nuclear import of ORF28 can be mediated by the v...
Source: Virus Research - April 21, 2024 Category: Virology Authors: Huang-Shen Lin Cheng-Han Li Lee-Wen Chen Shie- Shan Wang Li- Yu Chen Chien-Hui Hung Chun-Liang Lin Pey-Jium Chang Source Type: research
SARS-CoV-2 superinfection in CD14 < sup > + < /sup > monocytes with latent human cytomegalovirus (HCMV) promotes inflammatory cascade
This study is a first step toward understanding the mechanism that may facilitate increased COVID-19 disease severity in patients infected with SARS-CoV-2 and HCMV.PMID:38642618 | DOI:10.1016/j.virusres.2024.199375 (Source: Virus Research)
Source: Virus Research - April 20, 2024 Category: Virology Authors: Shannon Harger Payen Kabita Adhikari Juli Petereit Timsy Uppal Cyprian C Rossetto Subhash C Verma Source Type: research
Interaction and antiviral treatment of coinfection between SARS-CoV-2 and influenza in vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Interaction and Antiviral Treatment of Coinfection between SARS-CoV-2 and Influenza in Vitro
CONCLUSIONS: In coinfection, the replication of SARS-CoV-2 would be interfered by influenza H1N1. Compared with molnupiravir or baloxavir monotherapy, treatment with a combination of molnupiravir and baloxavir should be considered for early treatment in patients with SARS-CoV-2 and influenza coinfection.PMID:38621598 | DOI:10.1016/j.virusres.2024.199371 (Source: Virus Research)
Source: Virus Research - April 15, 2024 Category: Virology Authors: Danlei Liu Ka-Yi Leung Hoi-Yan Lam Ruiqi Zhang Yujing Fan Xiaochun Xie Kwok-Hung Chan Ivan Fan-Ngai Hung Source Type: research
Immunogenicity of Novel vB_EcoS_NBD2 Bacteriophage-Originated Nanotubes as a Carrier for Peptide-Based Vaccines
This study investigated, for the first time, the immunogenicity of long and flexible polytubes formed by yeast-expressed tail tube protein gp39 of bacteriophage vB_EcoS_NBD2 and evaluated their ability to elicit an immune response against the inserted protein fragments. Protein gp39-based polytubes induced humoral immune response in mice, even without the use of adjuvant. Bioinformatics analysis guided the selection of protein fragments from Acinetobacter baumannii for insertion into the C-terminus of gp39. Chimeric polytubes, displaying 28-amino acid long OmpA protein fragment, induced IgG response against OmpA protein fr...
Source: Virus Research - April 13, 2024 Category: Virology Authors: Aliona Avi žinienė Indr ė Dalgėdienė Julija Armalyt ė Rasa Petraityt ė-Burneikienė Source Type: research
