Generation and characterization of monoclonal antibodies against the hemagglutinin of H3N2 influenza A viruses
Virus Res. 2022 May 17:198815. doi: 10.1016/j.virusres.2022.198815. Online ahead of print.ABSTRACTSeasonal influenza viruses are highly contagious, leading to 290,000-650,000 mortalities every year globally. Among the influenza viruses, influenza A virus (H3N2) has attracted much attention due to its high frequency of antigenic variations, resulting in poor protection by vaccination. We generated a panel of murine neutralizing monoclonal antibodies (mAbs) against A/Texas/50/2012 (H3N2) and identified the relevant epitopes that potentially influence the antigenicity by selecting mAb-resistant mutants. The epitopes were main...
Source: Virus Research - May 20, 2022 Category: Virology Authors: Fan Yang Linwei Zhu Fumin Liu Linfang Cheng Hangping Yao Nanping Wu Haibo Wu Lanjuan Li Source Type: research

An immunological glimpse of human virus peptides: distance from self, MHC class I binding, Proteasome Cleveage, TAP Transport and sequence composition entropy
Virus Res. 2022 May 16:198814. doi: 10.1016/j.virusres.2022.198814. Online ahead of print.ABSTRACTAdaptive immune response is triggered when specific pathogen peptides called epitopes are recognised as exogenous according to the paradigm of self/non-self. To be recognized by immune cells, epitopes have to be exposed (presented) on the surface of the cell. Predicting if a peptide is exposed is important to shed light on the rules that govern immune response, and thus to identify potential targets, and to design vaccine and drugs. We focused on peptides exposed on cell surface and made accessible to immune system through the...
Source: Virus Research - May 19, 2022 Category: Virology Authors: Daniele Santonia Giovanni Felici Source Type: research

An immunological glimpse of human virus peptides: distance from self, MHC class I binding, Proteasome Cleveage, TAP Transport and sequence composition entropy
Virus Res. 2022 May 16:198814. doi: 10.1016/j.virusres.2022.198814. Online ahead of print.ABSTRACTAdaptive immune response is triggered when specific pathogen peptides called epitopes are recognised as exogenous according to the paradigm of self/non-self. To be recognized by immune cells, epitopes have to be exposed (presented) on the surface of the cell. Predicting if a peptide is exposed is important to shed light on the rules that govern immune response, and thus to identify potential targets, and to design vaccine and drugs. We focused on peptides exposed on cell surface and made accessible to immune system through the...
Source: Virus Research - May 19, 2022 Category: Virology Authors: Daniele Santonia Giovanni Felici Source Type: research

Identification of novel integration sites for BLV proviral DNA in cancer driver genes in cattle with persistent lymphocytosis
Virus Res. 2022 May 15:198813. doi: 10.1016/j.virusres.2022.198813. Online ahead of print.ABSTRACTEnzootic bovine leukosis is one of the unsolved problems of cattle breeding in many countries. The etiological agent of the disease is the bovine leukemia virus (BLV) - an oncogenic retrovirus, that infects B-lymphocytes in cattle. The number and genetic content of BLV provirus integration sites in the bovine genome were reported to can be used as an early diagnostic sign of leukemogenesis in the infected cattle, but patterns of BLV provirus integration into the bovine genome and associations between genomic features of the in...
Source: Virus Research - May 18, 2022 Category: Virology Authors: Anna V Babii Anna L Arkhipova Svetlana N Kovalchuk Source Type: research

Identification of novel integration sites for BLV proviral DNA in cancer driver genes in cattle with persistent lymphocytosis
Virus Res. 2022 May 15:198813. doi: 10.1016/j.virusres.2022.198813. Online ahead of print.ABSTRACTEnzootic bovine leukosis is one of the unsolved problems of cattle breeding in many countries. The etiological agent of the disease is the bovine leukemia virus (BLV) - an oncogenic retrovirus, that infects B-lymphocytes in cattle. The number and genetic content of BLV provirus integration sites in the bovine genome were reported to can be used as an early diagnostic sign of leukemogenesis in the infected cattle, but patterns of BLV provirus integration into the bovine genome and associations between genomic features of the in...
Source: Virus Research - May 18, 2022 Category: Virology Authors: Anna V Babii Anna L Arkhipova Svetlana N Kovalchuk Source Type: research

New insights from the virome of Halyomorpha halys (St ål, 1855)
Virus Res. 2022 May 14:198802. doi: 10.1016/j.virusres.2022.198802. Online ahead of print.ABSTRACTHalyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae), the brown marmorated stink bug, is an invasive pentatomid native to East-Asia, and introduced worldwide in recent times. It is a polyphagous pest with approximately 300 host plants, which, due to its plasticity, reproductive and feeding behaviour, long-distance flight, and walking as well as human-mediated dispersal ability, is able to cause significant economic and ecological damage. In several cases pest control mediated by insecticide treatments leads to unsatisfact...
Source: Virus Research - May 17, 2022 Category: Virology Authors: L Giovannini G Mazza W Chitarra G Sabbatini-Peverieri C Sonnati P F Roversi L Nerva Source Type: research

Genomic and biological characteristics of a newly isolated lytic bacteriophage PZJ0206 infecting the Enterobacter cloacae
Virus Res. 2022 May 13:198800. doi: 10.1016/j.virusres.2022.198800. Online ahead of print.ABSTRACTEnterobacter cloacae is an opportunistic pathogen that is widely distributed in the environment, often causing hospital and community-acquired infections. The limited phage resources and poor genomic data hinder the understanding of the lysis mechanism of Enterobacter cloacae phages. In the present study, we characterized a newly discovered lytic phage PZJ0206 that infects an E. cloacae strain isolated from sewage water into the Pearl River in Guangdong, China. The morphological characteristics of the icosahedral head and shor...
Source: Virus Research - May 16, 2022 Category: Virology Authors: Zhuobo Li Wei Wang Bo Ma Jiayue Yin Chaoqun Hu Peng Luo Yanhong Wang Source Type: research

Genomic and biological characteristics of a newly isolated lytic bacteriophage PZJ0206 infecting the Enterobacter cloacae
Virus Res. 2022 May 13:198800. doi: 10.1016/j.virusres.2022.198800. Online ahead of print.ABSTRACTEnterobacter cloacae is an opportunistic pathogen that is widely distributed in the environment, often causing hospital and community-acquired infections. The limited phage resources and poor genomic data hinder the understanding of the lysis mechanism of Enterobacter cloacae phages. In the present study, we characterized a newly discovered lytic phage PZJ0206 that infects an E. cloacae strain isolated from sewage water into the Pearl River in Guangdong, China. The morphological characteristics of the icosahedral head and shor...
Source: Virus Research - May 16, 2022 Category: Virology Authors: Zhuobo Li Wei Wang Bo Ma Jiayue Yin Chaoqun Hu Peng Luo Yanhong Wang Source Type: research

The invasion biology of tomato begomoviruses in Costa Rica reveals neutral synergism that may lead to increased disease pressure and economic loss
Virus Res. 2022 May 12:198793. doi: 10.1016/j.virusres.2022.198793. Online ahead of print.ABSTRACTSince the late 1980s, tomato production in Costa Rica has been affected by diseases caused by whitefly-transmitted begomoviruses. The first was tomato yellow mottle virus (ToYMoV), a locally evolved New World (NW) bipartite begomovirus associated with the tomato yellow mottle disease (ToYMoD). In the late 1990s, the invasive NW bipartite tomato leaf curl Sinaloa virus (ToLCSiV) was detected in Costa Rica and has become established and associated with ToYMoD. Finally, the invasive Old World (OW) monopartite tomato yellow leaf c...
Source: Virus Research - May 15, 2022 Category: Virology Authors: Minor R Maliano Maria R Rojas Monica A Macedo Natalia Barboza Robert L Gilbertson Source Type: research

The invasion biology of tomato begomoviruses in Costa Rica reveals neutral synergism that may lead to increased disease pressure and economic loss
Virus Res. 2022 May 12:198793. doi: 10.1016/j.virusres.2022.198793. Online ahead of print.ABSTRACTSince the late 1980s, tomato production in Costa Rica has been affected by diseases caused by whitefly-transmitted begomoviruses. The first was tomato yellow mottle virus (ToYMoV), a locally evolved New World (NW) bipartite begomovirus associated with the tomato yellow mottle disease (ToYMoD). In the late 1990s, the invasive NW bipartite tomato leaf curl Sinaloa virus (ToLCSiV) was detected in Costa Rica and has become established and associated with ToYMoD. Finally, the invasive Old World (OW) monopartite tomato yellow leaf c...
Source: Virus Research - May 15, 2022 Category: Virology Authors: Minor R Maliano Maria R Rojas Monica A Macedo Natalia Barboza Robert L Gilbertson Source Type: research

Efficient Recovery of Attenuated Canine Distemper Virus from cDNA
Virus Res. 2022 May 11:198796. doi: 10.1016/j.virusres.2022.198796. Online ahead of print.ABSTRACTTo provide insights into the biology of the attenuated canine distemper virus (CDV) Onderstepoort (OP) strain (large plaque forming variant), design next-generation multivalent vaccines, or further investigate its promising potential as an oncolytic vector, we employed contemporary modifications to establish an efficient OP-CDV-based reverse genetics platform. Successful viral rescue was obtained however only upon recovery of a completely conserved charged residue (V13E) residing at the N-terminal region of the large protein (...
Source: Virus Research - May 14, 2022 Category: Virology Authors: Marianne Wyss Vaiva Gradauskaite Nadine Ebert Volker Thiel Andreas Zurbriggen Philippe Plattet Source Type: research

Effect of the host genotype at a Porcine Reproductive and Respiratory Syndrome (PRRS) resistance marker on evolution of the modified-live PRRS vaccine virus in pigs
Virus Res. 2022 May 11:198809. doi: 10.1016/j.virusres.2022.198809. Online ahead of print.ABSTRACTPorcine Reproductive and Respiratory Syndrome, Porcine Reproductive and Respiratory Syndrome Virus, PRRS, Whole genome sequencing, quasispecies, WUR allele.PMID:35568091 | DOI:10.1016/j.virusres.2022.198809 (Source: Virus Research)
Source: Virus Research - May 14, 2022 Category: Virology Authors: Raymond R R Rowland Tyler Doerksen Andrea Lu Maureen Sheahan Joan Lunney Jack Dekkers Rachel M Palinski Source Type: research

Efficient Recovery of Attenuated Canine Distemper Virus from cDNA
Virus Res. 2022 May 11:198796. doi: 10.1016/j.virusres.2022.198796. Online ahead of print.ABSTRACTTo provide insights into the biology of the attenuated canine distemper virus (CDV) Onderstepoort (OP) strain (large plaque forming variant), design next-generation multivalent vaccines, or further investigate its promising potential as an oncolytic vector, we employed contemporary modifications to establish an efficient OP-CDV-based reverse genetics platform. Successful viral rescue was obtained however only upon recovery of a completely conserved charged residue (V13E) residing at the N-terminal region of the large protein (...
Source: Virus Research - May 14, 2022 Category: Virology Authors: Marianne Wyss Vaiva Gradauskaite Nadine Ebert Volker Thiel Andreas Zurbriggen Philippe Plattet Source Type: research

Effect of the host genotype at a Porcine Reproductive and Respiratory Syndrome (PRRS) resistance marker on evolution of the modified-live PRRS vaccine virus in pigs
Virus Res. 2022 May 11:198809. doi: 10.1016/j.virusres.2022.198809. Online ahead of print.ABSTRACTPorcine Reproductive and Respiratory Syndrome, Porcine Reproductive and Respiratory Syndrome Virus, PRRS, Whole genome sequencing, quasispecies, WUR allele.PMID:35568091 | DOI:10.1016/j.virusres.2022.198809 (Source: Virus Research)
Source: Virus Research - May 14, 2022 Category: Virology Authors: Raymond R R Rowland Tyler Doerksen Andrea Lu Maureen Sheahan Joan Lunney Jack Dekkers Rachel M Palinski Source Type: research

A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate virulence and a promising application perspective
Virus Res. 2022 May 10;316:198801. doi: 10.1016/j.virusres.2022.198801. Online ahead of print.ABSTRACTNADC30-like porcine reproductive and respiratory syndrome virus (PRRSV), which is highly homologous to the NADC30 strain isolated in the United States. The NADC30-like PRRSV was first reported in 2014 in China, where it spread and gradually caused an epidemic. Currently, growing research has shown that NADC30-like strains have greater propensity to recombine with other PRRSV strains, particularly the PPRSV vaccine virus used clinically, making the prevention and control of PRRSV highly complex. To carry out an in-depth mol...
Source: Virus Research - May 13, 2022 Category: Virology Authors: Zhengda Cao Jinxia Chen Liwei Li Jiachen Liu Wu Tong Yanjun Zhou Guangzhi Tong Guihua Wang Fei Gao Source Type: research