Studying longitudinal neutralising antibody levels against Equid herpesvirus 1 in experimentally infected horses using a novel pseudotype based assay
Virus Res. 2023 Nov 4:199262. doi: 10.1016/j.virusres.2023.199262. Online ahead of print.ABSTRACTInfection with equid herpesvirus 1 (EHV-1), a DNA virus of the Herpesviridae family represents a significant welfare issue in horses and a great impact on the equine industry. During EHV-1 infection, entry of the virus into different cell types is complex due to the presence of twelve glycoproteins (GPs) on the viral envelope. To investigate virus entry mechanisms, specific combinations of GPs were pseudotyped onto lentiviral vectors. Pseudotyped virus (PV) particles bearing gB, gD, gH and gL were able to transduce several targ...
Source: Virus Research - November 6, 2023 Category: Virology Authors: Cecilia Di Genova Gabrielle Sutton Romain Paillot Nigel Temperton St éphane Pronost Simon D Scott Source Type: research
Detection of a cluster of Omicron's BA.4 sublineage in Northern Senegal and identification of the first XAS recombinant variant in Senegal
Virus Res. 2023 Nov 3:199259. doi: 10.1016/j.virusres.2023.199259. Online ahead of print.ABSTRACTIn Senegal, since its first detection in early March 2020, genomic surveillance of SARS-CoV-2 isolates has led to the identification of the emergence of the Omicron BA.4 and BA.5 sublineages from early June 2022. To investigate the origin of a cluster of cases in Northern Senegal on July 2022, isolates were analysed using Next-generation sequencing and phylogeny. Our data provided evidence of the origin of the cluster of BA.4 cases from a XAS recombinant, that is to date, the first reported sequence of this variant from Senegal...
Source: Virus Research - November 5, 2023 Category: Virology Authors: Martin Faye Modeste Name Faye Babacar Ndiaye Moussa Mo ïse Diagne Safietou Sankhe Ndeye Mari ème Top Amadou Diallo Cheikh Loucoubar Ndongo Dia Amadou Alpha Sall Ousmane Faye Source Type: research
Isolation, characterization, and phylogenetic analysis of two new porcine parvovirus 1 isolates from Northern China
In this study, we isolated two new PPV1 (HLJ202108-Y and SDLC202109) from northern China and sequenced their whole genomes. The new isolates were found to have three amino acid substitutions (K195R, K562R, and S578P) in nonstructural protein 1. The VP2 amino acid site contained nine nonsynonymous substitutions, including six substitutions of the Kresse strain corresponding to the NADL-2 strain and three substitutions of A414S, S436T, and N555K. Genetic evolution analysis was conducted on 107 reference sequences available in the GenBank database, and 4-5 PPV1 taxa were defined. The new isolates were in the same phylogenetic...
Source: Virus Research - November 3, 2023 Category: Virology Authors: Huiwei Deng Guangyi Cong Hongfeng Wang Zedong Hu Da Shi Hongyan Shi Changyou Xia Fang Fu Li Feng Source Type: research
Veratramine Inhibits Porcine Epidemic Diarrhea Virus Entry through Macropinocytosis by Suppressing PI3K/Akt Pathway
This study aimed to identify the antiviral mechanism of Veratramine (VAM), which actively inhibits PEDV replication with a 50% inhibitory concentration (IC50) of ∼ 5 µM. Upon VAM treatment, both PEDV-nucleocapsid (N) protein level and virus titer decreased significantly. The time-of-addition assay results showed that VAM could inhibit PEDV replication by blocking viral entry. Importantly, VAM could inhibit PEDV-induced phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) activity and further suppress micropinocytosis, which is required for PEDV entry. In addition, PI3K inhibitor LY294002 showed anti-PEDV activity b...
Source: Virus Research - November 3, 2023 Category: Virology Authors: Huan Chen Pu Zhao Caisheng Zhang Xin Ming Chaofeng Zhang Yong-Sam Jung Yingjuan Qian Source Type: research
Rotavirus-induced lncRNA SLC7A11-AS1 promotes ferroptosis by targeting cystine/glutamate antiporter xCT (SLC7A11) to facilitate virus infection
Virus Res. 2023 Nov 1:199261. doi: 10.1016/j.virusres.2023.199261. Online ahead of print.ABSTRACTRotavirus (RV) is the primary etiological agent of virus-associated gastroenteritis in infants, causing 200,000 childhood death annually. Despite the availability of vaccines, rotaviral diarrhea continues to be a severe issue in underdeveloped nations in Asia and Africa. The situation demands continual studies on host-rotavirus interactions to understand disease pathogenesis and develop effective antiviral therapeutics. Long non-coding RNAs (lncRNAs), which are a subset of non-coding RNAs of more than 200 nucleotides in length,...
Source: Virus Research - November 3, 2023 Category: Virology Authors: Shreya Banerjee Rakesh Sarkar Arpita Mukherjee Suvrotoa Mitra Animesh Gope Mamta Chawla-Sarkar Source Type: research
Tetrandrine (TET) inhibits African swine fever virus entry into cells by blocking the PI3K/Akt pathway
Virus Res. 2023 Nov 4;339:199258. doi: 10.1016/j.virusres.2023.199258. Online ahead of print.ABSTRACTAfrican Swine Fever Virus (ASFV) infection causes an acute and highly contagious disease in swine, resulting in significant economic losses and societal harm worldwide. Currently, there are no effective vaccines or antiviral drugs available for ASFV. Tetrandrine (TET) is extracted from the traditional Chinese herb Stephania tetrandrae, possesses diverse biological functions such as anti-inflammatory, anti-tumor, and antiviral activities. The study comprehensively evaluated the anti-ASFV effect of TET and validated it throug...
Source: Virus Research - November 3, 2023 Category: Virology Authors: Bingxu Qian Yongxin Hu Cong Liu Dongxia Zheng Xiuju Han Mingxia Gong Yanli Zou Dexin Zeng Kai Liao Yurun Miao Xiaodong Wu Jianjun Dai Zhiliang Wang Feng Xue Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40 % amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78 % at the full-genome level and 87 % ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40 % amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78 % at the full-genome level and 87 % ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40 % amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78 % at the full-genome level and 87 % ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40 % amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78 % at the full-genome level and 87 % ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40 % amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78 % at the full-genome level and 87 % ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40% amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78% at the full-genome level and 87% at ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
In this study, we report the discovery of four endornaviruses tentatively named Setosphaeria turcica endornavirus 1 (StEV1), Setosphaeria turcica endornavirus 2 (StEV2), Bipolaris maydis endornavirus 1 (BmEV1), and Bipolaris maydis endornavirus 2 (BmEV2). StEV1 and StEV2 infect Exserohilum turcicum, while BmEV1 and BmEV2 infect Bipolaris maydis. The four viruses encode a polyprotein with less than 40% amino acid sequence identity to other known endornaviruses, indicating that they are novel, previously undescribed endornaviruses. However, StEV1 and BmEV1 share a sequence identity of 78% at the full-genome level and 87% at ...
Source: Virus Research - October 28, 2023 Category: Virology Authors: Peng Wang Shouhui Pan Yun Zheng Xin Pan Zhongnan Gao Xuan Zhou Fei Dai Zhanbiao Li Qingchao Deng Shouguo Fang Haoran Wang Songbai Zhang Source Type: research
Integrin αvβ1 facilitates ACE2-mediated entry of SARS-CoV-2
This study aimed to investigate how integrins facilitate the ACE2-mediated cellular entry of SARS-CoV-2. We first tested the susceptibility of a panel of human cell lines to SARS-CoV-2 infection using the spike protein pseudotyped virus assay and examined the expression levels of integrins in these cell lines by qPCR, western blot and flow cytometry. We found that integrin αvβ1 was highly enriched in the SARS-CoV-2 susceptible cell lines. Additional studies demonstrated that RGD (403-405)→AAA mutant was defective in binding to integrin αvβ1 compared to its wild type counterpart, and anti-αvβ1 integrin antibodies si...
Source: Virus Research - October 26, 2023 Category: Virology Authors: Zeqiong Cai Han Bai Doudou Ren Biyun Xue Yijia Liu Tian Gong Xuan Zhang Peng Zhang Junsheng Zhu Binyin Shi Chengsheng Zhang Source Type: research
Integrin αvβ1 facilitates ACE2-mediated entry of SARS-CoV-2
This study aimed to investigate how integrins facilitate the ACE2-mediated cellular entry of SARS-CoV-2. We first tested the susceptibility of a panel of human cell lines to SARS-CoV-2 infection using the spike protein pseudotyped virus assay and examined the expression levels of integrins in these cell lines by qPCR, western blot and flow cytometry. We found that integrin αvβ1 was highly enriched in the SARS-CoV-2 susceptible cell lines. Additional studies demonstrated that RGD (403-405)→AAA mutant was defective in binding to integrin αvβ1 compared to its wild type counterpart, and anti-αvβ1 integrin antibodies si...
Source: Virus Research - October 26, 2023 Category: Virology Authors: Zeqiong Cai Han Bai Doudou Ren Biyun Xue Yijia Liu Tian Gong Xuan Zhang Peng Zhang Junsheng Zhu Binyin Shi Chengsheng Zhang Source Type: research
