Conessine inhibits enveloped viruses replication through up-regulating cholesterol level
Virus Res. 2023 Oct 4:199234. doi: 10.1016/j.virusres.2023.199234. Online ahead of print.ABSTRACTDengue virus (DENV) is one of the most prevalent arthropod-borne diseases. It may cause dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS), while no effective vaccines and drugs are available. Our study demonstrated that conessine exhibits broad antiviral activity against several enveloped viruses, including DENV, vesicular stomatitis virus, and herpes simplex virus. In addition, conessine has no direct destructive effect on the integrity or infectivity of virions. Both pre-treatment and post-treatment with conessin...
Source: Virus Research - October 6, 2023 Category: Virology Authors: Shili Zhou Jie Li Xiaomei Ling Shirui Dong Zhen Zhang Ming Li Source Type: research
Conessine inhibits enveloped viruses replication through up-regulating cholesterol level
Virus Res. 2023 Oct 4:199234. doi: 10.1016/j.virusres.2023.199234. Online ahead of print.ABSTRACTDengue virus (DENV) is one of the most prevalent arthropod-borne diseases. It may cause dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS), while no effective vaccines and drugs are available. Our study demonstrated that conessine exhibits broad antiviral activity against several enveloped viruses, including DENV, vesicular stomatitis virus, and herpes simplex virus. In addition, conessine has no direct destructive effect on the integrity or infectivity of virions. Both pre-treatment and post-treatment with conessin...
Source: Virus Research - October 6, 2023 Category: Virology Authors: Shili Zhou Jie Li Xiaomei Ling Shirui Dong Zhen Zhang Ming Li Source Type: research
EBV infection of primary colonic epithelial cells causes inflammation, DDR and autophagy dysregulation, effects that may predispose to IBD and carcinogenesis
In this study, we show for the first time that EBV infects primary colonic epithelial cells (HCoEpC), promotes pro-inflammatory cytokine secretion and activates molecular pathways bridging inflammation and cancer, such as ERK1/2. These effects, occurring in the course of the lytic phase of the viral life cycle, led to DDR and autophagy dysregulation. Such cellular responses, playing a key role in the maintenance of proteostasis and genome integrity, are essential to prevent carcinogenesis. Interestingly, we found that the use of the demethylating agent 5-AZA could counteract most of the effects induced by EBV infection in ...
Source: Virus Research - October 5, 2023 Category: Virology Authors: Roberta Santarelli Lorenzo Evangelista Chiara Pompili Salvatore Lo Presti Alberto Rossi Andrea Arena Aurelia Gaeta Roberta Gonnella Maria Saveria Gilardini Montani Mara Cirone Source Type: research
Corrigendum to 'A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe' [Virus Research 291 (2021) 198221]
Virus Res. 2023 Oct 3:199228. doi: 10.1016/j.virusres.2023.199228. Online ahead of print.NO ABSTRACTPMID:37798193 | DOI:10.1016/j.virusres.2023.199228 (Source: Virus Research)
Source: Virus Research - October 5, 2023 Category: Virology Authors: Yukiyo Sato Sabitree Shahi Paul Telengech Sakae Hisano Carolina Cornejo Daniel Rigling Hideki Kondo Nobuhiro Suzuki Source Type: research
EBV infection of primary colonic epithelial cells causes inflammation, DDR and autophagy dysregulation, effects that may predispose to IBD and carcinogenesis
In this study, we show for the first time that EBV infects primary colonic epithelial cells (HCoEpC), promotes pro-inflammatory cytokine secretion and activates molecular pathways bridging inflammation and cancer, such as ERK1/2. These effects, occurring in the course of the lytic phase of the viral life cycle, led to DDR and autophagy dysregulation. Such cellular responses, playing a key role in the maintenance of proteostasis and genome integrity, are essential to prevent carcinogenesis. Interestingly, we found that the use of the demethylating agent 5-AZA could counteract most of the effects induced by EBV infection in ...
Source: Virus Research - October 5, 2023 Category: Virology Authors: Roberta Santarelli Lorenzo Evangelista Chiara Pompili Salvatore Lo Presti Alberto Rossi Andrea Arena Aurelia Gaeta Roberta Gonnella Maria Saveria Gilardini Montani Mara Cirone Source Type: research
Corrigendum to 'A new tetra-segmented splipalmivirus with divided RdRP domains from Cryphonectria naterciae, a fungus found on chestnut and cork oak trees in Europe' [Virus Research 291 (2021) 198221]
Virus Res. 2023 Oct 3:199228. doi: 10.1016/j.virusres.2023.199228. Online ahead of print.NO ABSTRACTPMID:37798193 | DOI:10.1016/j.virusres.2023.199228 (Source: Virus Research)
Source: Virus Research - October 5, 2023 Category: Virology Authors: Yukiyo Sato Sabitree Shahi Paul Telengech Sakae Hisano Carolina Cornejo Daniel Rigling Hideki Kondo Nobuhiro Suzuki Source Type: research
Natural recombination of the torque teno canis virus within the ORF1, -2, and -3 genes and the untranslated region
This study screened fecal samples from domestic dogs in Henan Province. There was a positivity rate of 16.5% (19/115) for TTCaV. Four nearly complete TTCaV genomes, namely Canine/HeNan/4, 5, 6, and 13/2019, were obtained from the 19 positive fecal samples, whose genome sequence was obtained using gap-filling PCR. Sequence analysis revealed two unique amino acid mutation sites in the TTCaV strains, K278Q (compared with the first isolate Cf-TTV10 in Japan) and V/L268I (compared with the TTCaV strain from southern China). Subsequently, 17 near full-length TTCaV genome sequences were subjected to phylogenetic and recombination...
Source: Virus Research - October 4, 2023 Category: Virology Authors: Xuyang Wang Jiaojiao Cheng Yujie Jiang Jiajun Ou Siqi Cai Liang Xu Lintao Zhong Yuqing Xiao Xuerui Hu Gang Lu Liguo Yuan Source Type: research
Natural recombination of the torque teno canis virus within the ORF1, -2, and -3 genes and the untranslated region
This study screened fecal samples from domestic dogs in Henan Province. There was a positivity rate of 16.5% (19/115) for TTCaV. Four nearly complete TTCaV genomes, namely Canine/HeNan/4, 5, 6, and 13/2019, were obtained from the 19 positive fecal samples, whose genome sequence was obtained using gap-filling PCR. Sequence analysis revealed two unique amino acid mutation sites in the TTCaV strains, K278Q (compared with the first isolate Cf-TTV10 in Japan) and V/L268I (compared with the TTCaV strain from southern China). Subsequently, 17 near full-length TTCaV genome sequences were subjected to phylogenetic and recombination...
Source: Virus Research - October 4, 2023 Category: Virology Authors: Xuyang Wang Jiaojiao Cheng Yujie Jiang Jiajun Ou Siqi Cai Liang Xu Lintao Zhong Yuqing Xiao Xuerui Hu Gang Lu Liguo Yuan Source Type: research
An enterovirus A71 virus-like particle with replaced loops confers partial cross-protection in mice
In conclusion, the successful construction of EV-A71-VLPCHI3 suggested that loop-dependent heterologous protection could be transferred by loops replacement on the surface of viral capsid. This strategy may also supplement the development of multivalent vaccines against other infectious viral diseases.PMID:37788720 | DOI:10.1016/j.virusres.2023.199235 (Source: Virus Research)
Source: Virus Research - October 3, 2023 Category: Virology Authors: Xin Liu Hanyu Zhu Mei Wang Ning Zhang Jing Wang Wenbian Tan Guochuan Wu Pei Yu Hongbo Liu Qiliang Liu Source Type: research
An enterovirus A71 virus-like particle with replaced loops confers partial cross-protection in mice
In conclusion, the successful construction of EV-A71-VLPCHI3 suggested that loop-dependent heterologous protection could be transferred by loops replacement on the surface of viral capsid. This strategy may also supplement the development of multivalent vaccines against other infectious viral diseases.PMID:37788720 | DOI:10.1016/j.virusres.2023.199235 (Source: Virus Research)
Source: Virus Research - October 3, 2023 Category: Virology Authors: Xin Liu Hanyu Zhu Mei Wang Ning Zhang Jing Wang Wenbian Tan Guochuan Wu Pei Yu Hongbo Liu Qiliang Liu Source Type: research
In-cell Western assay to quantify infection with pathogenic orthohantavirus Puumala virus in replication kinetics and antiviral drug testing
Virus Res. 2023 Sep 29;337:199230. doi: 10.1016/j.virusres.2023.199230. Online ahead of print.ABSTRACTHemorrhagic fever with renal syndrome (HFRS) represents a serious zoonotic disease caused by orthohantaviruses in Eurasia. A specific antiviral therapy is not available. HFRS is characterized by acute kidney injury (AKI) with often massive proteinuria. Infection of kidney cells may contribute to the clinical picture. However, orthohantaviral replication in kidney cells is not well characterized. Therefore, we aimed to perform a reliable high-throughput assay that allows the quantification of infection rates and testing of ...
Source: Virus Research - September 30, 2023 Category: Virology Authors: Christian Nusshag Pamela Schreiber Josephine Uhrig Martin Zeier Ellen Krautkr ämer Source Type: research
In-cell Western assay to quantify infection with pathogenic orthohantavirus Puumala virus in replication kinetics and antiviral drug testing
Virus Res. 2023 Sep 29;337:199230. doi: 10.1016/j.virusres.2023.199230. Online ahead of print.ABSTRACTHemorrhagic fever with renal syndrome (HFRS) represents a serious zoonotic disease caused by orthohantaviruses in Eurasia. A specific antiviral therapy is not available. HFRS is characterized by acute kidney injury (AKI) with often massive proteinuria. Infection of kidney cells may contribute to the clinical picture. However, orthohantaviral replication in kidney cells is not well characterized. Therefore, we aimed to perform a reliable high-throughput assay that allows the quantification of infection rates and testing of ...
Source: Virus Research - September 30, 2023 Category: Virology Authors: Christian Nusshag Pamela Schreiber Josephine Uhrig Martin Zeier Ellen Krautkr ämer Source Type: research
Establishment and application of a point-of-care testing and diagnosis method for early immediate expression gene IE1 of cytomegalovirus in maternal urine based on isothermal amplification
CONCLUSIONS: To sum up, we developed a diagnostic method for HCMV based on UL123 gene combined with RPA and LFS, which is low dependent on equipment, fast, sensitive and specific, provide reference for point-of-care testing HCMV in grass-roots laboratories and remote areas.PMID:37769815 | DOI:10.1016/j.virusres.2023.199229 (Source: Virus Research)
Source: Virus Research - September 28, 2023 Category: Virology Authors: Chu Chu Shijiao Yu Fanli Min Lizhou Sun Meilin Liu Qian Meng Source Type: research
Biophysical characterization of the cetacean morbillivirus haemagglutinin glycoprotein
Virus Res. 2023 Sep 27;336:199231. doi: 10.1016/j.virusres.2023.199231. Online ahead of print.ABSTRACTCetacean morbillivirus (CeMV) is an enveloped, non-segmented, negative-stranded RNA virus that infects marine mammals, spreading across species and causing lethal disease outbreaks worldwide. Among the eight proteins encoded by the CeMV genome, the haemagglutinin (H) glycoprotein is responsible for the virus attachment to host cell receptors. CeMV H represents an attractive target for antiviral and diagnostic research, yet the elucidation of the molecular mechanisms underlying its role in infection and inter-species transm...
Source: Virus Research - September 28, 2023 Category: Virology Authors: Luca Zinzula Judith Scholz Istv án Nagy Giovanni Di Guardo Massimiliano Orsini Source Type: research
Biophysical characterization of the cetacean morbillivirus haemagglutinin glycoprotein
Virus Res. 2023 Sep 27;336:199231. doi: 10.1016/j.virusres.2023.199231. Online ahead of print.ABSTRACTCetacean morbillivirus (CeMV) is an enveloped, non-segmented, negative-stranded RNA virus that infects marine mammals, spreading across species and causing lethal disease outbreaks worldwide. Among the eight proteins encoded by the CeMV genome, the haemagglutinin (H) glycoprotein is responsible for the virus attachment to host cell receptors. CeMV H represents an attractive target for antiviral and diagnostic research, yet the elucidation of the molecular mechanisms underlying its role in infection and inter-species transm...
Source: Virus Research - September 28, 2023 Category: Virology Authors: Luca Zinzula Judith Scholz Istv án Nagy Giovanni Di Guardo Massimiliano Orsini Source Type: research
