Establishment and application of a point-of-care testing and diagnosis method for early immediate expression gene IE1 of cytomegalovirus in maternal urine based on isothermal amplification
CONCLUSIONS: To sum up, we developed a diagnostic method for HCMV based on UL123 gene combined with RPA and LFS, which is low dependent on equipment, fast, sensitive and specific, provide reference for point-of-care testing HCMV in grass-roots laboratories and remote areas.PMID:37769815 | DOI:10.1016/j.virusres.2023.199229 (Source: Virus Research)
Source: Virus Research - September 28, 2023 Category: Virology Authors: Chu Chu Shijiao Yu Fanli Min Lizhou Sun Meilin Liu Qian Meng Source Type: research
Biophysical characterization of the cetacean morbillivirus haemagglutinin glycoprotein
Virus Res. 2023 Sep 26:199231. doi: 10.1016/j.virusres.2023.199231. Online ahead of print.ABSTRACTCetacean morbillivirus (CeMV) is an enveloped, non-segmented, negative-stranded RNA virus that infects marine mammals, spreading across species and causing lethal disease outbreaks worldwide. Among the eight proteins encoded by the CeMV genome, the haemagglutinin (H) glycoprotein is responsible for the virus attachment to host cell receptors. CeMV H represents an attractive target for antiviral and diagnostic research, yet the elucidation of the molecular mechanisms underlying its role in infection and inter-species transmissi...
Source: Virus Research - September 28, 2023 Category: Virology Authors: Luca Zinzula Judith Scholz Istv án Nagy Giovanni Di Guardo Massimiliano Orsini Source Type: research
Establishment and application of a point-of-care testing and diagnosis method for early immediate expression gene IE1 of cytomegalovirus in maternal urine based on isothermal amplification
CONCLUSIONS: To sum up, we developed a diagnostic method for HCMV based on UL123 gene combined with RPA and LFS, which is low dependent on equipment, fast, sensitive and specific, provide reference for point-of-care testing HCMV in grass-roots laboratories and remote areas.PMID:37769815 | DOI:10.1016/j.virusres.2023.199229 (Source: Virus Research)
Source: Virus Research - September 28, 2023 Category: Virology Authors: Chu Chu Shijiao Yu Fanli Min Lizhou Sun Meilin Liu Qian Meng Source Type: research
Exploring the enzymatic activity of depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2
In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods to modify the reducing end of the oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage with TFA, followed by TLC and HPLC-MS analyses, revealed that RaK2 gp531 is a β-(1→4)-endoglucosidase. The enzyme specifically recognizes and cleaves the capsular polysaccharide (CPS) of the Klebsiella pneumoniae K54 serotype, releasing K-unit monomers (the main product), dimers, and trimers. Depolymerase gp531 remains active from 10 to 5...
Source: Virus Research - September 23, 2023 Category: Virology Authors: Algirdas Noreika Jonita Stankevi čiūtė Rasa Rutkien ė Rolandas Me škys Laura Kalinien ė Source Type: research
Exploring the enzymatic activity of depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2
In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods to modify the reducing end of the oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage with TFA, followed by TLC and HPLC-MS analyses, revealed that RaK2 gp531 is a β-(1→4)-endoglucosidase. The enzyme specifically recognizes and cleaves the capsular polysaccharide (CPS) of the Klebsiella pneumoniae K54 serotype, releasing K-unit monomers (the main product), dimers, and trimers. Depolymerase gp531 remains active from 10 to 5...
Source: Virus Research - September 23, 2023 Category: Virology Authors: Algirdas Noreika Jonita Stankevi čiūtė Rasa Rutkien ė Rolandas Me škys Laura Kalinien ė Source Type: research
Exploring the Enzymatic Activity of Depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2
In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods to modify the reducing end of the oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage with TFA, followed by TLC and HPLC-MS analyses, revealed that RaK2 gp531 is a β-(1→4)-endoglucosidase. The enzyme specifically recognizes and cleaves the capsular polysaccharide (CPS) of the Klebsiella pneumoniae K54 serotype, releasing K-unit monomers (the main product), dimers, and trimers. Depolymerase gp531 remains active from 10 to 5...
Source: Virus Research - September 23, 2023 Category: Virology Authors: Algirdas Noreika Jonita Stankevi čiūtė Rasa Rutkien ė Rolandas Me škys Laura Kaliniene Source Type: research
Exploring the Enzymatic Activity of Depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2
In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods to modify the reducing end of the oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage with TFA, followed by TLC and HPLC-MS analyses, revealed that RaK2 gp531 is a β-(1→4)-endoglucosidase. The enzyme specifically recognizes and cleaves the capsular polysaccharide (CPS) of the Klebsiella pneumoniae K54 serotype, releasing K-unit monomers (the main product), dimers, and trimers. Depolymerase gp531 remains active from 10 to 5...
Source: Virus Research - September 23, 2023 Category: Virology Authors: Algirdas Noreika Jonita Stankevi čiūtė Rasa Rutkien ė Rolandas Me škys Laura Kaliniene Source Type: research
Exploring the Enzymatic Activity of Depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2
In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods to modify the reducing end of the oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage with TFA, followed by TLC and HPLC-MS analyses, revealed that RaK2 gp531 is a β-(1→4)-endoglucosidase. The enzyme specifically recognizes and cleaves the capsular polysaccharide (CPS) of the Klebsiella pneumoniae K54 serotype, releasing K-unit monomers (the main product), dimers, and trimers. Depolymerase gp531 remains active from 10 to 5...
Source: Virus Research - September 23, 2023 Category: Virology Authors: Algirdas Noreika Jonita Stankevi čiūtė Rasa Rutkien ė Rolandas Me škys Laura Kaliniene Source Type: research
Characterization and genomic analysis of Stutzerimonas stutzeri phage vB_PstS_ZQG1, representing a novel viral genus
Virus Res. 2023 Sep 20:199226. doi: 10.1016/j.virusres.2023.199226. Online ahead of print.ABSTRACTStutzerimonas stutzeri is an opportunistic pathogenic bacterium belonging to the Gammaproteobacteria, exhibiting wide distribution in the environment and playing significant ecological roles such as nitrogen fixation or pollutant degradation. Despite its ecological importance, only two S. stutzeri phages have been isolated to date. Here, a novel S. stutzeri phage, vB_PstS_ZQG1, was isolated from the surface seawater of Qingdao, China. Transmission electron microscopy analysis indicates that vB_PstS_ZQG1 has a morphology charac...
Source: Virus Research - September 22, 2023 Category: Virology Authors: Fuyue Ge Ruizhe Guo Yantao Liang Ying Chen Hongbing Shao Yeong Yik Sung Wen Jye Mok Li Lian Wong Andrew McMinn Min Wang Source Type: research
Identification of γ-Fagarine as a novel antiviral agent against respiratory virus (hMPV) infection
In this study, we aim to investigate the effect of γ-Fagarine on hMPV infection and explore its underlying molecular mechanisms. Vero-E6 and 16HBE cells were used as cell models. Virus replication and microcosm character were explored in Vero-E6 cells. Then, the antiviral activities were investigated by quantitative real-time PCR (RT-qPCR), western blotting (WB), and indirect immunofluorescence assays (IFAs) in Vero-E6 and 16HBE. Potential mechanisms of γ-Fagarine related to HSPG and lysosome pH were assessed in 16HBE cells. Lastly, a virus-infected mouse model was established and antiviral assay in vivo was conducted. ...
Source: Virus Research - September 21, 2023 Category: Virology Authors: Jinhua Li Yao Zhao Ying Dai Junning Zhao Source Type: research
Identification of γ-Fagarine as a novel antiviral agent against respiratory virus (hMPV) infection
In this study, we aim to investigate the effect of γ-Fagarine on hMPV infection and explore its underlying molecular mechanisms. Vero-E6 and 16HBE cells were used as cell models. Virus replication and microcosm character were explored in Vero-E6 cells. Then, the antiviral activities were investigated by quantitative real-time PCR (RT-qPCR), western blotting (WB), and indirect immunofluorescence assays (IFAs) in Vero-E6 and 16HBE. Potential mechanisms of γ-Fagarine related to HSPG and lysosome pH were assessed in 16HBE cells. Lastly, a virus-infected mouse model was established and antiviral assay in vivo was conducted. ...
Source: Virus Research - September 21, 2023 Category: Virology Authors: Jinhua Li Yao Zhao Ying Dai Junning Zhao Source Type: research
Targeting HNRNPA2B1 inhibits enterovirus 71 replication in SK-N-SH cells
CONCLUSION: HNRNPA2B1 protein redistributed from nucleus to cytoplasm and is highly expressed in the cytoplasm during EV-71 infection. Inhibition of HNRNPA2B1 levels effectively inhibits EV-71 replication in SK-N-SH cells.PMID:37716669 | DOI:10.1016/j.virusres.2023.199224 (Source: Virus Research)
Source: Virus Research - September 16, 2023 Category: Virology Authors: Jing Wu Jian Lu Lingxiang Mao Meiqin Xu Lu Dai Yun Wang Source Type: research
Making sense of flavivirus non-strctural protein 1 in innate immune evasion and inducing tissue-specific damage
Virus Res. 2023 Sep 16;336:199222. doi: 10.1016/j.virusres.2023.199222. Online ahead of print.ABSTRACTFlaviviruses include medically important mosquito-borne pathogens, such as Zika virus (ZIKV), Japanese encephalitis virus (JEV), dengue virus (DENV) and West Nile virus (WNV), that cause hundreds of millions of infections each year. Currently, there are no approved effect therapies against mosquito-borne flaviviruses. The flaviviruses encoded nonstructural protein 1 (NS1) is a secreted glycoprotein widely involved in viral replication, immune evasion, and directly causing tissue-specific damage during flaviviruses infectio...
Source: Virus Research - September 16, 2023 Category: Virology Authors: Quan Zeng Jiaqi Liu Chenlin Hao Bo Zhang Honglei Zhang Source Type: research
Targeting HNRNPA2B1 inhibits enterovirus 71 replication in SK-N-SH cells
CONCLUSION: HNRNPA2B1 protein redistributed from nucleus to cytoplasm and is highly expressed in the cytoplasm during EV-71 infection. Inhibition of HNRNPA2B1 levels effectively inhibits EV-71 replication in SK-N-SH cells.PMID:37716669 | DOI:10.1016/j.virusres.2023.199224 (Source: Virus Research)
Source: Virus Research - September 16, 2023 Category: Virology Authors: Jing Wu Jian Lu Lingxiang Mao Meiqin Xu Lu Dai Yun Wang Source Type: research
Making sense of flavivirus non-strctural protein 1 in innate immune evasion and inducing tissue-specific damage
Virus Res. 2023 Sep 16;336:199222. doi: 10.1016/j.virusres.2023.199222. Online ahead of print.ABSTRACTFlaviviruses include medically important mosquito-borne pathogens, such as Zika virus (ZIKV), Japanese encephalitis virus (JEV), dengue virus (DENV) and West Nile virus (WNV), that cause hundreds of millions of infections each year. Currently, there are no approved effect therapies against mosquito-borne flaviviruses. The flaviviruses encoded nonstructural protein 1 (NS1) is a secreted glycoprotein widely involved in viral replication, immune evasion, and directly causing tissue-specific damage during flaviviruses infectio...
Source: Virus Research - September 16, 2023 Category: Virology Authors: Quan Zeng Jiaqi Liu Chenlin Hao Bo Zhang Honglei Zhang Source Type: research
