Two genes encoded by mulberry crinkle leaf virus (MCLV): the V4 gene enhances viral replication, and the V5 gene is needed for MCLV infection in Nicotiana benthamiana
Virus Res. 2023 Dec 1:199288. doi: 10.1016/j.virusres.2023.199288. Online ahead of print.ABSTRACTMulberry crinkle leaf virus (MCLV) is a member of the genus Mulcrilevirus, family Geminiviridae. The expression and functions of the V4 and V5 genes encoded by the MCLV genome remain unknown. Here, we confirmed the expression of V4 and V5 by analyzing the V4 and V5 mRNAs and the promoter activity of individual ORFs upstream sequences. The functions of V4 and V5 were investigated by constructing Agrobacterium-mediated infectious clones of wild-type MCLV variant П (MCLV vII), MCLVwt and MCLV vП mutants, such as MCLVmV4 (start c...
Source: Virus Research - December 3, 2023 Category: Virology Authors: Tao-Tao Han Jia-Xuan Tang Miao Fang Peng Zhang Pei- Yu Han Zhen-Ni Yin Yu Ma Jian Zhang Quan-You Lu Source Type: research
Seven new mycoviruses identified from isolated ascomycetous macrofungi
Virus Res. 2023 Dec 1:199290. doi: 10.1016/j.virusres.2023.199290. Online ahead of print.ABSTRACTMycoviruses have been described in all major fungal taxonomic groups. There has been much focus on commercially cultivated basidiomycetous macrofungi, while attention to viruses from ascomycetous macrofungi is lacking. Therefore, in this study, we conducted viral screening against fungal mycelia that were regenerated from ascomycetous macrofungi using agarose gel electrophoresis (AGE) and fragmented and primer-ligated dsRNA sequencing (FLDS). Among the 57 isolates, four isolates were detected with virus-like bands through scree...
Source: Virus Research - December 3, 2023 Category: Virology Authors: Yan-Jie Zhao Tsuyoshi Hosoya Syunichi Urayama Daisuke Hagiwara Source Type: research
Analysis of the molecular determinants for furin cleavage of the spike protein S1/S2 site in defined strains of the prototype coronavirus murine hepatitis virus (MHV)
Virus Res. 2023 Dec 1:199283. doi: 10.1016/j.virusres.2023.199283. Online ahead of print.ABSTRACTWe analyzed the spike protein S1/S2 cleavage of selected strains of a prototype coronavirus, mouse hepatitis virus (MHV) by the cellular protease furin, in order to understand the structural requirements underlying the sequence selectivity of the scissile segment. The probability of cleavage of selected MHV strains was first evaluated from furin cleavage scores predicted by the ProP computer software, and then cleavage was measured experimentally with a fluorogenic peptide cleavage assay consisting of S1/S2 peptide mimics and p...
Source: Virus Research - December 3, 2023 Category: Virology Authors: Annette Choi Ekaterina D Kots Deanndria T Singleton Harel Weinstein Gary R Whittaker Source Type: research
IgM-specific linear epitopes on the E2 protein for serodiagnosis of Chikungunya
Virus Res. 2023 Nov 30:199292. doi: 10.1016/j.virusres.2023.199292. Online ahead of print.ABSTRACTChikungunya virus (CHIKV) and Dengue virus (DENV) are vector-borne diseases transmitted by Aedes aegypti and Aedes albopictus that pose a significant threat to global public health. Cases of acute Chikungunya fever often present similar clinical symptoms to other vector-borne diseases such as Dengue fever. In regions where multiple vector-borne diseases coexist, CHIKV is often overlooked or misdiagnosed as Dengue virus, West Nile virus, Zika virus or other viral infections, which delays its prevention and control. However, IgM...
Source: Virus Research - December 2, 2023 Category: Virology Authors: Qianlin Li Jun Dai Yongxia Shi Qiang Deng Conghui Liao Jicheng Huang Jiahai Lu Source Type: research
Enterovirus D68 vRNA induces type III IFN production via MDA5
In this study, we show that EV-D68 infection stimulates Calu-3 cells to secrete IFN-λ. The transfection of EV-D68 viral RNA (vRNA) stimulated IFN-λ via MDA5. Furthermore, our findings provide evidence that EV-D68 infection also induces MDA5- IRF3/IRF7-mediated IFN-λ. In addition, we discovered that EV-D68 infection downregulated MDA5 expression. Knockdown of MDA5 increased EV-D68 replication in Calu-3 cells. Finally, we demonstrated that the IFN-λ1 and IFN-λ2/3 proteins effectively inhibit EV-D68 infection in respiratory epithelial cells. In summary, our study shows that EV-D68 induces type III IFN production via the ...
Source: Virus Research - December 1, 2023 Category: Virology Authors: Chi-Chong Chio Hio-Wai Chan Shih-Hsiang Chen Hsing-I Huang Source Type: research
Enterovirus D68 vRNA induces type III IFN production via MDA5
In this study, we show that EV-D68 infection stimulates Calu-3 cells to secrete IFN-λ. The transfection of EV-D68 viral RNA (vRNA) stimulated IFN-λ via MDA5. Furthermore, our findings provide evidence that EV-D68 infection also induces MDA5- IRF3/IRF7-mediated IFN-λ. In addition, we discovered that EV-D68 infection downregulated MDA5 expression. Knockdown of MDA5 increased EV-D68 replication in Calu-3 cells. Finally, we demonstrated that the IFN-λ1 and IFN-λ2/3 proteins effectively inhibit EV-D68 infection in respiratory epithelial cells. In summary, our study shows that EV-D68 induces type III IFN production via the ...
Source: Virus Research - December 1, 2023 Category: Virology Authors: Chi-Chong Chio Hio-Wai Chan Shih-Hsiang Chen Hsing-I Huang Source Type: research
Tracking SARS-CoV-2 Variants During the 2023 Flu Season and Beyond in Lebanon
CONCLUSIONS: The timely detection and characterization of SARS-CoV-2 variants is important to reduce transmission through established disease control measures and to avoid introductions into animal populations that could lead to serious public health implications.PMID:38036064 | DOI:10.1016/j.virusres.2023.199289 (Source: Virus Research)
Source: Virus Research - November 30, 2023 Category: Virology Authors: Ibrahim Al Kodsi Douaa El Rayes Jad Koweyes Charbel Al Khoury Kelven Rahy Sergio Thoumi Marc Chamoun Hoda Haddad Jacques Mokhbat Sima Tokajian Source Type: research
Tracking SARS-CoV-2 Variants During the 2023 Flu Season and Beyond in Lebanon
CONCLUSIONS: The timely detection and characterization of SARS-CoV-2 variants is important to reduce transmission through established disease control measures and to avoid introductions into animal populations that could lead to serious public health implications.PMID:38036064 | DOI:10.1016/j.virusres.2023.199289 (Source: Virus Research)
Source: Virus Research - November 30, 2023 Category: Virology Authors: Ibrahim Al Kodsi Douaa El Rayes Jad Koweyes Charbel Al Khoury Kelven Rahy Sergio Thoumi Marc Chamoun Hoda Haddad Jacques Mokhbat Sima Tokajian Source Type: research
ASFV epitope mapping by high density peptides microarrays
Virus Res. 2023 Nov 27:199287. doi: 10.1016/j.virusres.2023.199287. Online ahead of print.ABSTRACTAfrican swine fever (ASF) is an acute, highly contagious and deadly infectious disease. It is a threat to animal health with major potential economic and societal impact. Despite decades of ASF vaccine research, still some gaps in knowledge are hindering the development of a functional vaccine. Worth mentioning are gaps in understanding the mechanism of ASF infection and immunity, as well as the fact that - in case of this disease - virus proteins, so-called protective antigens, responsible for inducing protective immune respo...
Source: Virus Research - November 29, 2023 Category: Virology Authors: Clo é Desmet Bruna Coelho-Cruz Dora Mehn Pascal Colpo Ana Ruiz-Moreno Source Type: research
Gambogic acid inhibits HBx-mediated hepatitis B virus replication by targeting the DTX1-Notch signaling pathway
CONCLUSIONS: Herein, we discovered GA, which is a natural bioactive compound that targets HBx to inhibit hepatitis B virus replication by activating the DTX1-Notch signaling pathway.PMID:38029800 | DOI:10.1016/j.virusres.2023.199273 (Source: Virus Research)
Source: Virus Research - November 29, 2023 Category: Virology Authors: Xu Wen Dian Li Peng Chen Ming Tan Hui Zhang Yuting Liu Jihua Ren Shengtao Cheng Source Type: research
Oxidative stress enhances rotavirus oncolysis in breast cancer and leukemia, except in melanoma with abundant matrix
CONCLUSIONS: Oxidative stress promotes oncolytic rotavirus infection in breast cancer and acute lymphoblastic leukemia cells, suggesting potential utility in combination with radiotherapy or chemotherapy due to their shared induction of intracellular oxidative stress.PMID:38013142 | DOI:10.1016/j.virusres.2023.199285 (Source: Virus Research)
Source: Virus Research - November 28, 2023 Category: Virology Authors: Marvi Guerrero Juan Hern ández Luis Gomez Carlos Guerrero Source Type: research
A nano-luciferase expressing human coronavirus OC43 for countermeasure development
Virus Res. 2023 Nov 26:199286. doi: 10.1016/j.virusres.2023.199286. Online ahead of print.ABSTRACTThe genetic diversity of the coronavirus (CoV) family poses a significant challenge for drug discovery and development. Traditional antiviral drugs often target specific viral proteins from specific viruses which limits their use, especially against novel emerging viruses. Antivirals with broad-spectrum activity overcome this limitation by targeting highly conserved regions or catalytic domains within viral proteins that are essential for replication. For rapid identification of small molecules with broad antiviral activity, a...
Source: Virus Research - November 28, 2023 Category: Virology Authors: Meghan V Diefenbacher Thomas J Baric David R Martinez Ralph S Baric Nicholas J Catanzaro Timothy P Sheahan Source Type: research
Oxidative stress enhances rotavirus oncolysis in breast cancer and leukemia, except in melanoma with abundant matrix
CONCLUSIONS: Oxidative stress promotes oncolytic rotavirus infection in breast cancer and acute lymphoblastic leukemia cells, suggesting potential utility in combination with radiotherapy or chemotherapy due to their shared induction of intracellular oxidative stress.PMID:38013142 | DOI:10.1016/j.virusres.2023.199285 (Source: Virus Research)
Source: Virus Research - November 28, 2023 Category: Virology Authors: Marvi Guerrero Juan Hern ández Luis Gomez Carlos Guerrero Source Type: research
A nano-luciferase expressing human coronavirus OC43 for countermeasure development
Virus Res. 2023 Nov 26:199286. doi: 10.1016/j.virusres.2023.199286. Online ahead of print.ABSTRACTThe genetic diversity of the coronavirus (CoV) family poses a significant challenge for drug discovery and development. Traditional antiviral drugs often target specific viral proteins from specific viruses which limits their use, especially against novel emerging viruses. Antivirals with broad-spectrum activity overcome this limitation by targeting highly conserved regions or catalytic domains within viral proteins that are essential for replication. For rapid identification of small molecules with broad antiviral activity, a...
Source: Virus Research - November 28, 2023 Category: Virology Authors: Meghan V Diefenbacher Thomas J Baric David R Martinez Ralph S Baric Nicholas J Catanzaro Timothy P Sheahan Source Type: research
Oxidative Stress Enhances Rotavirus Oncolysis in Breast Cancer and Leukemia, except in Melanoma with Abundant Matrix
CONCLUSIONS: Oxidative stress promotes oncolytic rotavirus infection in breast cancer and acute lymphoblastic leukemia cells, suggesting potential utility in combination with radiotherapy or chemotherapy due to their shared induction of intracellular oxidative stress.PMID:38013142 | DOI:10.1016/j.virusres.2023.199285 (Source: Virus Research)
Source: Virus Research - November 28, 2023 Category: Virology Authors: Marvi Guerrero Juan Hern ández Luis Gomez Carlos Guerrero Source Type: research
