Angiotensin Converting Enzyme 2 Does Not Facilitate Porcine Epidemic Diarrhea Virus Entry into Porcine Intestinal Epithelial Cells and Inhibits It-induced Inflammatory Injury by Promoting STAT1 Phosphorylation
Virus Res. 2023 Dec 11:199300. doi: 10.1016/j.virusres.2023.199300. Online ahead of print.ABSTRACTACE2 has been confirmed to be a functional receptor for SARS-CoV and SARS-CoV-2, but research on animal coronaviruses, especially PEDV, are still unknown. The present study investigated whether ACE2 plays a role in receptor recognition and subsequent infection during PEDV invasion of host cells. IPEC-J2 cells stably expressing porcine ACE2 did not increase the production of PEDV-N but inhibited its expression. Porcine ACE2 knockout cells was generated by CRISPR/Cas9 genome editing in IPEC-J2 cells. The expression of PEDV-N did...
Source: Virus Research - December 13, 2023 Category: Virology Authors: Zhiqiang Li Xueqing Chen Chang Ma Xinyu Du Yuanshu Zhang Source Type: research
Angiotensin Converting Enzyme 2 Does Not Facilitate Porcine Epidemic Diarrhea Virus Entry into Porcine Intestinal Epithelial Cells and Inhibits It-induced Inflammatory Injury by Promoting STAT1 Phosphorylation
Virus Res. 2023 Dec 11:199300. doi: 10.1016/j.virusres.2023.199300. Online ahead of print.ABSTRACTACE2 has been confirmed to be a functional receptor for SARS-CoV and SARS-CoV-2, but research on animal coronaviruses, especially PEDV, are still unknown. The present study investigated whether ACE2 plays a role in receptor recognition and subsequent infection during PEDV invasion of host cells. IPEC-J2 cells stably expressing porcine ACE2 did not increase the production of PEDV-N but inhibited its expression. Porcine ACE2 knockout cells was generated by CRISPR/Cas9 genome editing in IPEC-J2 cells. The expression of PEDV-N did...
Source: Virus Research - December 13, 2023 Category: Virology Authors: Zhiqiang Li Xueqing Chen Chang Ma Xinyu Du Yuanshu Zhang Source Type: research
Peroxide derivatives as SARS-CoV-2 entry inhibitors
Virus Res. 2023 Dec 12;340:199295. doi: 10.1016/j.virusres.2023.199295. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Host cell invasion is mediated by the interaction of the viral spike protein (S) with human angiotensin-converting enzyme 2 (ACE2) through the receptor-binding domain (RBD). In this work, bio-layer interferometry (BLI) was used to screen a series of fifty-two peroxides, including aminoperoxides and bridged 1,2,4 - trioxolanes (ozonides), with the aim of identifying small molecules that inte...
Source: Virus Research - December 11, 2023 Category: Virology Authors: Ding-Qi Zhang Qin-Hai Ma Meng-Chu Yang Yulia Yu Belyakova Zi-Feng Yang Peter S Radulov Rui-Hong Chen Li-Jun Yang Jing-Yuan Wei Yu-Tong Peng Wu-Yan Zheng Ivan A Yaremenko Alexander O Terent'ev Paolo Coghi Vincent Kam Wai Wong Source Type: research
Peroxides Derivatives as SARS-CoV-2 Entry Inhibitors
Virus Res. 2023 Dec 9:199295. doi: 10.1016/j.virusres.2023.199295. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Host cell invasion is mediated by the interaction of the viral spike protein (S) with human angiotensin-converting enzyme 2 (ACE2) through the receptor-binding domain (RBD). In this work, bio-layer interferometry (BLI) was used to screen a series of fifty-two peroxides, including aminoperoxides and bridged 1,2,4 - trioxolanes (ozonides) classes, with the aim of identifying small molecules that i...
Source: Virus Research - December 11, 2023 Category: Virology Authors: Ding-Qi Zhang Qin-Hai Ma Meng-Chu Yang Yulia Yu Belyakova Zi-Feng Yang Peter S Radulov Rui-Hong Chen Li-Jun Yang Jing-Yuan Wei Yu-Tong Peng Wu-Yan Zheng Ivan A Yaremenko Alexander O Terent'ev Paolo Coghi Vincent Kam Wai Wong Source Type: research
The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein
This study describes the nuclear localization of citrus leprosis virus C (CiLV-C) capsid protein (p29) and its interaction with the fibrillarin (Fib2) within the nucleolar compartment and cell cytoplasm. Our results, obtained by computer predictions and laser scanning confocal microscopy analyses, including colocalization and bimolecular fluorescence complementation (BiFC) approaches, revealed that a fraction of the p29 is localized in the nucleus and colocalizes with the Fib2 in both the nucleolus and cytosol. The nuclear localization of p29 correlated with a smaller nucleus size. Furthermore, co-immunoprecipitation (Co-I...
Source: Virus Research - December 9, 2023 Category: Virology Authors: Mikhail Oliveira Leastro Vicente Pall ás Jes ús Ángel Sánchez-Navarro Source Type: research
The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein
This study describes the nuclear localization of citrus leprosis virus C (CiLV-C) capsid protein (p29) and its interaction with the fibrillarin (Fib2) within the nucleolar compartment and cell cytoplasm. Our results, obtained by computer predictions and laser scanning confocal microscopy analyses, including colocalization and bimolecular fluorescence complementation (BiFC) approaches, revealed that a fraction of the p29 is localized in the nucleus and colocalizes with the Fib2 in both the nucleolus and cytosol. The nuclear localization of p29 correlated with a smaller nucleus size. Furthermore, co-immunoprecipitation (Co-I...
Source: Virus Research - December 9, 2023 Category: Virology Authors: Mikhail Oliveira Leastro Vicente Pall ás Jes ús Ángel Sánchez-Navarro Source Type: research
LysZX4-NCA, a new endolysin with broad-spectrum antibacterial activity for topical treatment
Virus Res. 2023 Dec 8;340:199296. doi: 10.1016/j.virusres.2023.199296. Online ahead of print.ABSTRACTThe prevalence of multidrug-resistant highly virulent Klebsiella pneumoniae (MDR-hvKP) requires the development of new therapeutic agents. Herein, a novel lytic phage vB_KpnS_ZX4 against MDR-hvKP was discovered in hospital sewage. Phage vB_KpnS_ZX4 had a short latent period (5 min) and a large burst size (230 PFU/cell). It can rapidly reduce the number of bacteria in vitro and improve survival rates of bacteremic mice in vivo from 0 to 80 % with a single injection of 108 PFU. LysZX4, an endolysin derived from vB_KpnS_ZX4, e...
Source: Virus Research - December 8, 2023 Category: Virology Authors: Ping Li Mangmang Shen Wenjie Ma Xin Zhou Jiayin Shen Source Type: research
New anti-SARS-CoV-2 aminoadamantane compounds as antiviral candidates for the treatment of COVID-19
Virus Res. 2023 Dec 6:199291. doi: 10.1016/j.virusres.2023.199291. Online ahead of print.ABSTRACTHere, the antiviral activity of aminoadamantane derivatives were evaluated against SARS-CoV-2. The compounds exhibited low cytotoxicity to Vero, HEK293 and CALU-3 cells up to a concentration of 1,000 µM. The inhibitory concentration (IC50) of aminoadamantane was 39.71 µM in Vero CCL-81 cells and the derivatives showed significantly lower IC50 values, especially for compounds 3F4 (0.32 µM), 3F5 (0.44 µM) and 3E10 (1.28 µM). Additionally, derivatives 3F5 and 3E10 statistically reduced the fluorescence intensity of SARS-CoV-2...
Source: Virus Research - December 8, 2023 Category: Virology Authors: Daisymara Priscila de Almeida Marques Luis Adan Flores Andrade Erik Vinicius Sousa Reis Felipe Alves Clarindo Tha ís de Fátima Silva Moraes Karine Lima Louren ço Wellington Alves De Barros Nath ália Evelyn Morais Costa L ídia Maria de Andrade Ágata Source Type: research
LysZX4-NCA, a New Endolysin with Broad-spectrum Antibacterial Activity for Topical Treatment
Virus Res. 2023 Dec 6:199296. doi: 10.1016/j.virusres.2023.199296. Online ahead of print.ABSTRACTThe prevalence of multidrug-resistant highly virulent Klebsiella pneumoniae (MDR-hvKP) requires the development of new therapeutic agents. Herein, a novel lytic phage vB_KpnS_ZX4 against MDR-hvKP was discovered in hospital sewage. Phage vB_KpnS_ZX4 had a short latent period (5 min) and a large burst size (230 PFU/cell). It can rapidly reduce the number of bacteria in vitro and improve survival rates of bacteremic mice in vivo from 0 to 80% with a single injection of 108 PFU. LysZX4, an endolysin derived from vB_KpnS_ZX4, exhibi...
Source: Virus Research - December 8, 2023 Category: Virology Authors: Ping Li Mangmang Shen Wenjie Ma Xin Zhou Jiayin Shen Source Type: research
New anti-SARS-CoV-2 aminoadamantane compounds as antiviral candidates for the treatment of COVID-19
Virus Res. 2023 Dec 6:199291. doi: 10.1016/j.virusres.2023.199291. Online ahead of print.ABSTRACTHere, the antiviral activity of aminoadamantane derivatives were evaluated against SARS-CoV-2. The compounds exhibited low cytotoxicity to Vero, HEK293 and CALU-3 cells up to a concentration of 1,000 µM. The inhibitory concentration (IC50) of aminoadamantane was 39.71 µM in Vero CCL-81 cells and the derivatives showed significantly lower IC50 values, especially for compounds 3F4 (0.32 µM), 3F5 (0.44 µM) and 3E10 (1.28 µM). Additionally, derivatives 3F5 and 3E10 statistically reduced the fluorescence intensity of SARS-CoV-2...
Source: Virus Research - December 8, 2023 Category: Virology Authors: Daisymara Priscila de Almeida Marques Luis Adan Flores Andrade Erik Vinicius Sousa Reis Felipe Alves Clarindo Tha ís de Fátima Silva Moraes Karine Lima Louren ço Wellington Alves De Barros Nath ália Evelyn Morais Costa L ídia Maria de Andrade Ágata Source Type: research
Rapid increase in salivary IgA and broad recognition of spike protein following SARS-CoV-2 vaccination
In this study, saliva samples were collected from individuals that received SARS-CoV-2 vaccine, and immunoglobulin G (IgG), IgM, and IgA against whole spike protein and S1 protein were measured. IgA against whole spike protein increased significantly following vaccination, while IgA against S1 protein did not. Of note, the IgA response was evident two weeks after the first vaccine dose and continued to rise thereafter. On the contrary, IgG antibodies against S1 increased significantly at four weeks after vaccination. These results reveal the dynamics and recognition antigens of immunoglobulins in saliva, indicating the fun...
Source: Virus Research - December 6, 2023 Category: Virology Authors: Kenji Ota Hironori Sakai Daisuke Sasaki Fujiko Mitsumoto-Kaseida Kei Sakamoto Kosuke Kosai Hiroo Hasegawa Takahiro Takazono Koichi Izumikawa Hiroshi Mukae Mya Myat Ngwe Tun Kouichi Morita Katsunori Yanagihara Source Type: research
Rapid increase in salivary IgA and broad recognition of spike protein following SARS-CoV-2 vaccination
In this study, saliva samples were collected from individuals that received SARS-CoV-2 vaccine, and immunoglobulin G (IgG), IgM, and IgA against whole spike protein and S1 protein were measured. IgA against whole spike protein increased significantly following vaccination, while IgA against S1 protein did not. Of note, the IgA response was evident two weeks after the first vaccine dose and continued to rise thereafter. On the contrary, IgG antibodies against S1 increased significantly at four weeks after vaccination. These results reveal the dynamics and recognition antigens of immunoglobulins in saliva, indicating the fun...
Source: Virus Research - December 6, 2023 Category: Virology Authors: Kenji Ota Hironori Sakai Daisuke Sasaki Fujiko Mitsumoto-Kaseida Kei Sakamoto Kosuke Kosai Hiroo Hasegawa Takahiro Takazono Koichi Izumikawa Hiroshi Mukae Mya Myat Ngwe Tun Kouichi Morita Katsunori Yanagihara Source Type: research
Rapid increase in salivary IgA and broad recognition of spike protein following SARS-CoV-2 vaccination
In this study, saliva samples were collected from individuals that received SARS-CoV-2 vaccine, and immunoglobulin G (IgG), IgM, and IgA against whole spike protein and S1 protein were measured. IgA against whole spike protein increased significantly following vaccination, while IgA against S1 protein did not. Of note, the IgA response was evident two weeks after the first vaccine dose and continued to rise thereafter. On the contrary, IgG antibodies against S1 increased significantly at four weeks after vaccination. These results reveal the dynamics and recognition antigens of immunoglobulins in saliva, indicating the fun...
Source: Virus Research - December 6, 2023 Category: Virology Authors: Kenji Ota Hironori Sakai Daisuke Sasaki Fujiko Mitsumoto-Kaseida Kei Sakamoto Kosuke Kosai Hiroo Hasegawa Takahiro Takazono Koichi Izumikawa Hiroshi Mukae Mya Myat Ngwe Tun Kouichi Morita Katsunori Yanagihara Source Type: research
Two genes encoded by mulberry crinkle leaf virus (MCLV): the V4 gene enhances viral replication, and the V5 gene is needed for MCLV infection in Nicotiana benthamiana
Virus Res. 2023 Dec 1:199288. doi: 10.1016/j.virusres.2023.199288. Online ahead of print.ABSTRACTMulberry crinkle leaf virus (MCLV) is a member of the genus Mulcrilevirus, family Geminiviridae. The expression and functions of the V4 and V5 genes encoded by the MCLV genome remain unknown. Here, we confirmed the expression of V4 and V5 by analyzing the V4 and V5 mRNAs and the promoter activity of individual ORFs upstream sequences. The functions of V4 and V5 were investigated by constructing Agrobacterium-mediated infectious clones of wild-type MCLV variant П (MCLV vII), MCLVwt and MCLV vП mutants, such as MCLVmV4 (start c...
Source: Virus Research - December 3, 2023 Category: Virology Authors: Tao-Tao Han Jia-Xuan Tang Miao Fang Peng Zhang Pei- Yu Han Zhen-Ni Yin Yu Ma Jian Zhang Quan-You Lu Source Type: research
Seven new mycoviruses identified from isolated ascomycetous macrofungi
Virus Res. 2023 Dec 1:199290. doi: 10.1016/j.virusres.2023.199290. Online ahead of print.ABSTRACTMycoviruses have been described in all major fungal taxonomic groups. There has been much focus on commercially cultivated basidiomycetous macrofungi, while attention to viruses from ascomycetous macrofungi is lacking. Therefore, in this study, we conducted viral screening against fungal mycelia that were regenerated from ascomycetous macrofungi using agarose gel electrophoresis (AGE) and fragmented and primer-ligated dsRNA sequencing (FLDS). Among the 57 isolates, four isolates were detected with virus-like bands through scree...
Source: Virus Research - December 3, 2023 Category: Virology Authors: Yan-Jie Zhao Tsuyoshi Hosoya Syunichi Urayama Daisuke Hagiwara Source Type: research
