Interleukin-1 β promotes human metapneumovirus replication via activating the cGAS-STING pathway
CONCLUSION: This study found that IL-1β could promote hMPV replication through the cGAS-STING pathway, which has the potential to serve as a candidate to fight against hMPV infection, targeting IL-1β may be an effective new strategy to restrain virus replication.PMID:38431054 | DOI:10.1016/j.virusres.2024.199344 (Source: Virus Research)
Source: Virus Research - March 2, 2024 Category: Virology Authors: Guojin Wu Yueyan Zhang Linlin Niu Yuan Hu Yuting Yang Yao Zhao Source Type: research
Do bed bugs transmit human viruses, or do humans spread bed bugs and their viruses? A worldwide survey of the bed bug RNA virosphere
Virus Res. 2024 Feb 29:199349. doi: 10.1016/j.virusres.2024.199349. Online ahead of print.ABSTRACTBED BUGS (HEMIPTERA: : Cimicidae) are a globally distributed hematophagous pest that routinely feed on humans. Unlike many blood-sucking arthropods, they have never been linked to pathogen transmission in a natural setting, and despite increasing interest in their role as disease vectors, little is known about the viruses that bed bugs naturally harbor. Here, we present a global-scale survey of the bed bug RNA virosphere. We sequenced the metatranscriptomes of 22 individual bed bugs (Cimex lectularius and Cimex hemipterus) fro...
Source: Virus Research - March 2, 2024 Category: Virology Authors: Hunter K Walt Jonas G King Johnathan M Sheele Florencia Meyer Jose E Pietri Federico G Hoffmann Source Type: research
Deletion of the B125R gene in the African swine fever virus SY18 strain leads to an A104R frameshift mutation slightly attenuating virulence in domestic pigs
In this study, we discovered that B125R deletion did not affect recombinant virus rescue, nor did it hinder viral replication during the intermediate growth phase. Although the virulence of the recombinant strain harboring this deletion was attenuated, intramuscular inoculation of the recombinant virus in pigs at doses of 102 or 104 TCID50 resulted in mortality. Moreover, sequencing analysis of six recombinant strains obtained from three independent experiments consistently revealed an adenine insertion at the 47367-47375 bp site in the A104R gene due to the B125R deletion, leading to premature termination of this gene. In...
Source: Virus Research - February 29, 2024 Category: Virology Authors: Rongnian Zhu Ying Wang Han Zhang Jinjin Yang Jiaqi Fan Yanyan Zhang Yu Wang Qixuan Li Xintao Zhou Huixian Yue Yu Qi Shuchao Wang Teng Chen Shoufeng Zhang Rongliang Hu Source Type: research
Deletion of the B125R gene in the African swine fever virus SY18 strain leads to an A104R frameshift mutation slightly attenuating virulence in domestic pigs
In this study, we discovered that B125R deletion did not affect recombinant virus rescue, nor did it hinder viral replication during the intermediate growth phase. Although the virulence of the recombinant strain harboring this deletion was attenuated, intramuscular inoculation of the recombinant virus in pigs at doses of 102 or 104 TCID50 resulted in mortality. Moreover, sequencing analysis of six recombinant strains obtained from three independent experiments consistently revealed an adenine insertion at the 47367-47375 bp site in the A104R gene due to the B125R deletion, leading to premature termination of this gene. In...
Source: Virus Research - February 29, 2024 Category: Virology Authors: Rongnian Zhu Ying Wang Han Zhang Jinjin Yang Jiaqi Fan Yanyan Zhang Yu Wang Qixuan Li Xintao Zhou Huixian Yue Yu Qi Shuchao Wang Teng Chen Shoufeng Zhang Rongliang Hu Source Type: research
Deletion of the B125R gene in the African swine fever virus SY18 strain leads to an A104R frameshift mutation slightly attenuating virulence in domestic pigs
In this study, we discovered that B125R deletion did not affect recombinant virus rescue, nor did it hinder viral replication during the intermediate growth phase. Although the virulence of the recombinant strain harboring this deletion was attenuated, intramuscular inoculation of the recombinant virus in pigs at doses of 102 or 104 TCID50 resulted in mortality. Moreover, sequencing analysis of six recombinant strains obtained from three independent experiments consistently revealed an adenine insertion at the 47367-47375 bp site in the A104R gene due to the B125R deletion, leading to premature termination of this gene. In...
Source: Virus Research - February 29, 2024 Category: Virology Authors: Rongnian Zhu Ying Wang Han Zhang Jinjin Yang Jiaqi Fan Yanyan Zhang Yu Wang Qixuan Li Xintao Zhou Huixian Yue Yu Qi Shuchao Wang Teng Chen Shoufeng Zhang Rongliang Hu Source Type: research
African swine fever virus early protein pI73R suppresses the type-I IFN promoter activities
Virus Res. 2024 Feb 24:199342. doi: 10.1016/j.virusres.2024.199342. Online ahead of print.ABSTRACTAfrican swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-β promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dA:dT), a strong inducer of the cGAS-STING si...
Source: Virus Research - February 26, 2024 Category: Virology Authors: Danh Cong Lai Jayeshbhai Chaudhari Hiep L X Vu Source Type: research
African swine fever virus early protein pI73R suppresses the type-I IFN promoter activities
Virus Res. 2024 Feb 24:199342. doi: 10.1016/j.virusres.2024.199342. Online ahead of print.ABSTRACTAfrican swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-β promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dA:dT), a strong inducer of the cGAS-STING si...
Source: Virus Research - February 26, 2024 Category: Virology Authors: Danh Cong Lai Jayeshbhai Chaudhari Hiep L X Vu Source Type: research
African swine fever virus early protein pI73R suppresses the type-I IFN promoter activities
Virus Res. 2024 Feb 24:199342. doi: 10.1016/j.virusres.2024.199342. Online ahead of print.ABSTRACTAfrican swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-β promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dA:dT), a strong inducer of the cGAS-STING si...
Source: Virus Research - February 26, 2024 Category: Virology Authors: Danh Cong Lai Jayeshbhai Chaudhari Hiep L X Vu Source Type: research
Exploring COVID-19 Causal Genes through Disease-specific Cis-eQTLs
Virus Res. 2024 Feb 23:199341. doi: 10.1016/j.virusres.2024.199341. Online ahead of print.ABSTRACTGenome-wide association study (GWAS) analysis has exposed that genetic factors play important roles in COVID-19. Whereas a deeper understanding of the underlying mechanism of COVID-19 was hindered by the lack of expression of quantitative trait loci (eQTL) data specific for disease. To this end, we identified COVID-19-specific cis-eQTLs by integrating nucleotide sequence variations and RNA-Seq data from COVID-19 samples. These identified eQTLs have different regulatory effect on genes between patients and controls, indicating ...
Source: Virus Research - February 25, 2024 Category: Virology Authors: Sainan Zhang Ping Wang Lei Shi Chao Wang Zijun Zhu Changlu Qi Yubin Xie Shuofeng Yuan Liang Cheng Xin Yin Xue Zhang Source Type: research
Exploring COVID-19 Causal Genes through Disease-specific Cis-eQTLs
Virus Res. 2024 Feb 23:199341. doi: 10.1016/j.virusres.2024.199341. Online ahead of print.ABSTRACTGenome-wide association study (GWAS) analysis has exposed that genetic factors play important roles in COVID-19. Whereas a deeper understanding of the underlying mechanism of COVID-19 was hindered by the lack of expression of quantitative trait loci (eQTL) data specific for disease. To this end, we identified COVID-19-specific cis-eQTLs by integrating nucleotide sequence variations and RNA-Seq data from COVID-19 samples. These identified eQTLs have different regulatory effect on genes between patients and controls, indicating ...
Source: Virus Research - February 25, 2024 Category: Virology Authors: Sainan Zhang Ping Wang Lei Shi Chao Wang Zijun Zhu Changlu Qi Yubin Xie Shuofeng Yuan Liang Cheng Xin Yin Xue Zhang Source Type: research
Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species
Virus Res. 2024 Jan 2;339:199255. doi: 10.1016/j.virusres.2023.199255. Epub 2023 Nov 6.ABSTRACTSARS-CoV-2's genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicron's phenotyp...
Source: Virus Research - February 23, 2024 Category: Virology Authors: Manel Essaidi-Laziosi Francisco J P érez-Rodríguez Catia Alvarez Pascale Sattonnet-Roche Giulia Torriani Meriem Bekliz Kenneth Adea Matthias Lenk Tasnim Suliman Wolfgang Preiser Marcel A M üller Christian Drosten Laurent Kaiser Isabella Eckerle Source Type: research
Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species
Virus Res. 2024 Jan 2;339:199255. doi: 10.1016/j.virusres.2023.199255. Epub 2023 Nov 6.ABSTRACTSARS-CoV-2's genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicron's phenotyp...
Source: Virus Research - February 23, 2024 Category: Virology Authors: Manel Essaidi-Laziosi Francisco J P érez-Rodríguez Catia Alvarez Pascale Sattonnet-Roche Giulia Torriani Meriem Bekliz Kenneth Adea Matthias Lenk Tasnim Suliman Wolfgang Preiser Marcel A M üller Christian Drosten Laurent Kaiser Isabella Eckerle Source Type: research
Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species
Virus Res. 2024 Jan 2;339:199255. doi: 10.1016/j.virusres.2023.199255. Epub 2023 Nov 6.ABSTRACTSARS-CoV-2's genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicron's phenotyp...
Source: Virus Research - February 23, 2024 Category: Virology Authors: Manel Essaidi-Laziosi Francisco J P érez-Rodríguez Catia Alvarez Pascale Sattonnet-Roche Giulia Torriani Meriem Bekliz Kenneth Adea Matthias Lenk Tasnim Suliman Wolfgang Preiser Marcel A M üller Christian Drosten Laurent Kaiser Isabella Eckerle Source Type: research
Recruitment of the 40S ribosomal subunit by the West Nile virus 3' UTR promotes the cross-talk between the viral genomic ends for translation regulation
Virus Res. 2024 Feb 20:199340. doi: 10.1016/j.virusres.2024.199340. Online ahead of print.ABSTRACTFlaviviral RNA genomes are composed of discrete RNA structural units arranged in an ordered fashion and grouped into complex folded domains that regulate essential viral functions, e.g. replication and translation. This is achieved by adjusting the overall structure of the RNA genome via the establishment of inter- and intramolecular interactions. Translation regulation is likely the main process controlling flaviviral gene expression. Although the genomic 3' UTR is a key player in this regulation, little is known about the mo...
Source: Virus Research - February 22, 2024 Category: Virology Authors: Sara Esther Ramos-Lorente Beatriz Berzal-Herranz Cristina Romero-L ópez Alfredo Berzal-Herranz Source Type: research
Aichivirus A1 replicates in human intestinal epithelium and bronchial tissue: lung-gut axis?
Virus Res. 2024 Feb 17:199338. doi: 10.1016/j.virusres.2024.199338. Online ahead of print.ABSTRACTThe role of aichivirus A1 (AiV-A1) in acute gastroenteritis remains controversial and in vitro data illustrating its pathogenesis in suitable human models are scarce. Here, we demonstrate that AiV-A1 isolate A846/88 replicates in ApoA1- (absorptive) and Ki-67-positive (proliferative) enterocytes in stem cell-derived human small intestinal epithelium (HIE) as well as in patient biopsy samples, but not in any of the tested human cell lines. The infection did not result in tissue damage and did not trigger type I and type III int...
Source: Virus Research - February 19, 2024 Category: Virology Authors: Martin Jungbauer-Groznica Konstantin Wiese Irmgard Fischer Jan Markus Tsung-Hsien Chang Irene G ösler Heinrich Kowalski Dieter Blaas Antonio Real-Hohn Source Type: research
