Inhibition of Porcine Deltacoronavirus Entry and Replication by Cepharanthine
In conclusion, our results suggest that the anti-PDCoV activity of CEP is not only relies on competing the virus binding with pAPN, but also affects the proliferation of the virus in vitro by downregulating the excessive immune response caused by the virus and inducing autophagy. CEP emerges as a promising candidate for potential anti-PDCoV therapeutic development.PMID:38145807 | DOI:10.1016/j.virusres.2023.199303 (Source: Virus Research)
Source: Virus Research - December 25, 2023 Category: Virology Authors: Yumei Sun Zhongzhu Liu Shiyi Shen Mengjia Zhang Lina Liu Ahmed H Ghonaim Yongtao Li Shujun Zhang Wentao Li Source Type: research
Inhibition of Porcine Deltacoronavirus Entry and Replication by Cepharanthine
In conclusion, our results suggest that the anti-PDCoV activity of CEP is not only relies on competing the virus binding with pAPN, but also affects the proliferation of the virus in vitro by downregulating the excessive immune response caused by the virus and inducing autophagy. CEP emerges as a promising candidate for potential anti-PDCoV therapeutic development.PMID:38145807 | DOI:10.1016/j.virusres.2023.199303 (Source: Virus Research)
Source: Virus Research - December 25, 2023 Category: Virology Authors: Yumei Sun Zhongzhu Liu Shiyi Shen Mengjia Zhang Lina Liu Ahmed H Ghonaim Yongtao Li Shujun Zhang Wentao Li Source Type: research
Inhibition of Porcine Deltacoronavirus Entry and Replication by Cepharanthine
In conclusion, our results suggest that the anti-PDCoV activity of CEP is not only relies on competing the virus binding with pAPN, but also affects the proliferation of the virus in vitro by downregulating the excessive immune response caused by the virus and inducing autophagy. CEP emerges as a promising candidate for potential anti-PDCoV therapeutic development.PMID:38145807 | DOI:10.1016/j.virusres.2023.199303 (Source: Virus Research)
Source: Virus Research - December 25, 2023 Category: Virology Authors: Yumei Sun Zhongzhu Liu Shiyi Shen Mengjia Zhang Lina Liu Ahmed H Ghonaim Yongtao Li Shujun Zhang Wentao Li Source Type: research
The avian influenza A virus receptor SA- α2,3-Gal is expressed in the porcine nasal mucosa sustaining the pig as a mixing vessel for new influenza viruses
Virus Res. 2023 Dec 22:199304. doi: 10.1016/j.virusres.2023.199304. Online ahead of print.ABSTRACTInfluenza A viruses (IAVs) originate from wild birds but have on several occasions jumped host barriers and are now also circulating in humans and mammals. The IAV host receptors (glycans with galactose linked to a sialic acid (SA) in an α2,3 or α2,6 linkage) are crucial host factors restricting inter-species transmission. In general, avian-origin IAVs show a preference for SA-α2,3 (avian receptor), whereas IAVs isolated from humans and pigs prefer SA-α2,6 (human receptor). N-acetylneuraminic acid (Neu5Ac) and N-glycolylne...
Source: Virus Research - December 24, 2023 Category: Virology Authors: Charlotte Kristensen Lars E Larsen Ramona Trebbien Henrik E Jensen Source Type: research
The avian influenza A virus receptor SA- α2,3-Gal is expressed in the porcine nasal mucosa sustaining the pig as a mixing vessel for new influenza viruses
Virus Res. 2023 Dec 22:199304. doi: 10.1016/j.virusres.2023.199304. Online ahead of print.ABSTRACTInfluenza A viruses (IAVs) originate from wild birds but have on several occasions jumped host barriers and are now also circulating in humans and mammals. The IAV host receptors (glycans with galactose linked to a sialic acid (SA) in an α2,3 or α2,6 linkage) are crucial host factors restricting inter-species transmission. In general, avian-origin IAVs show a preference for SA-α2,3 (avian receptor), whereas IAVs isolated from humans and pigs prefer SA-α2,6 (human receptor). N-acetylneuraminic acid (Neu5Ac) and N-glycolylne...
Source: Virus Research - December 24, 2023 Category: Virology Authors: Charlotte Kristensen Lars E Larsen Ramona Trebbien Henrik E Jensen Source Type: research
Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication
We report that the numbers of PML NBs per nucleus were significantly downregulated during infection of PRRSV. The overexpression of all six isoforms of PML suppressed the PRRSV replication, and conversely, the silencing of PML gene expression enhanced the PRRSV replication. The suppression of PML NBs by the nsp1 protein was common in other member viruses of the family, represented by equine arteritis virus, lactate dehydrogenase elevating virus of mice, and simian hemorrhagic fever virus. Our study unveils a conserved viral strategy in arteriviruses for innate immune evasion.PMID:38104946 | DOI:10.1016/j.virusres.2023.1993...
Source: Virus Research - December 17, 2023 Category: Virology Authors: Chia-Ming Su Yu Fan Hung Junyu Tang Mingyuan Han Roger Everett Dongwan Yoo Source Type: research
Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication
We report that the numbers of PML NBs per nucleus were significantly downregulated during infection of PRRSV. The overexpression of all six isoforms of PML suppressed the PRRSV replication, and conversely, the silencing of PML gene expression enhanced the PRRSV replication. The suppression of PML NBs by the nsp1 protein was common in other member viruses of the family, represented by equine arteritis virus, lactate dehydrogenase elevating virus of mice, and simian hemorrhagic fever virus. Our study unveils a conserved viral strategy in arteriviruses for innate immune evasion.PMID:38104946 | DOI:10.1016/j.virusres.2023.1993...
Source: Virus Research - December 17, 2023 Category: Virology Authors: Chia-Ming Su Yu Fan Hung Junyu Tang Mingyuan Han Roger Everett Dongwan Yoo Source Type: research
Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication
We report that the numbers of PML NBs per nucleus were significantly downregulated during infection of PRRSV. The overexpression of all six isoforms of PML suppressed the PRRSV replication, and conversely, the silencing of PML gene expression enhanced the PRRSV replication. The suppression of PML NBs by the nsp1 protein was common in other member viruses of the family, represented by equine arteritis virus, lactate dehydrogenase elevating virus of mice, and simian hemorrhagic fever virus. Our study unveils a conserved viral strategy in arteriviruses for innate immune evasion.PMID:38104946 | DOI:10.1016/j.virusres.2023.1993...
Source: Virus Research - December 17, 2023 Category: Virology Authors: Chia-Ming Su Yu Fan Hung Junyu Tang Mingyuan Han Roger Everett Dongwan Yoo Source Type: research
Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication
We report that the numbers of PML NBs per nucleus were significantly downregulated during infection of PRRSV. The overexpression of all six isoforms of PML suppressed the PRRSV replication, and conversely, the silencing of PML gene expression enhanced the PRRSV replication. The suppression of PML NBs by the nsp1 protein was common in other member viruses of the family, represented by equine arteritis virus, lactate dehydrogenase elevating virus of mice, and simian hemorrhagic fever virus. Our study unveils a conserved viral strategy in arteriviruses for innate immune evasion.PMID:38104946 | DOI:10.1016/j.virusres.2023.1993...
Source: Virus Research - December 17, 2023 Category: Virology Authors: Chia-Ming Su Yu Fan Hung Junyu Tang Mingyuan Han Roger Everett Dongwan Yoo Source Type: research
Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication
We report that the numbers of PML NBs per nucleus were significantly downregulated during infection of PRRSV. The overexpression of all six isoforms of PML suppressed the PRRSV replication, and conversely, the silencing of PML gene expression enhanced the PRRSV replication. The suppression of PML NBs by the nsp1 protein was common in other member viruses of the family, represented by equine arteritis virus, lactate dehydrogenase elevating virus of mice, and simian hemorrhagic fever virus. Our study unveils a conserved viral strategy in arteriviruses for innate immune evasion.PMID:38104946 | DOI:10.1016/j.virusres.2023.1993...
Source: Virus Research - December 17, 2023 Category: Virology Authors: Chia-Ming Su Yu Fan Hung Junyu Tang Mingyuan Han Roger Everett Dongwan Yoo Source Type: research
Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication
We report that the numbers of PML NBs per nucleus were significantly downregulated during infection of PRRSV. The overexpression of all six isoforms of PML suppressed the PRRSV replication, and conversely, the silencing of PML gene expression enhanced the PRRSV replication. The suppression of PML NBs by the nsp1 protein was common in other member viruses of the family, represented by equine arteritis virus, lactate dehydrogenase elevating virus of mice, and simian hemorrhagic fever virus. Our study unveils a conserved viral strategy in arteriviruses for innate immune evasion.PMID:38104946 | DOI:10.1016/j.virusres.2023.1993...
Source: Virus Research - December 17, 2023 Category: Virology Authors: Chia-Ming Su Yu Fan Hung Junyu Tang Mingyuan Han Roger Everett Dongwan Yoo Source Type: research
Applying targeted gene hybridization capture to viruses with a focus to SARS-CoV-2
Virus Res. 2023 Dec 16;340:199293. doi: 10.1016/j.virusres.2023.199293. Online ahead of print.ABSTRACTAlthough next-generation sequencing technologies are advancing rapidly, many research topics often require selective sequencing of genomic regions of interest. In addition, sequencing low-titre viruses is challenging, especially for coronaviruses, which are the largest RNA viruses. Prior to sequencing, enrichment of viral particles can help to significantly increase target sequence information as well as avoid large sequencing efforts and, consequently, can increase sensitivity and reduce sequencing costs. Targeting nuclei...
Source: Virus Research - December 15, 2023 Category: Virology Authors: Andres Ceballos-Garzon Sophie Comtet-Marre Pierre Peyret Source Type: research
Applying Targeted Gene Hybridization Capture to Viruses with a Focus to SARS-CoV-2
Virus Res. 2023 Dec 13:199293. doi: 10.1016/j.virusres.2023.199293. Online ahead of print.ABSTRACTAlthough next-generation sequencing technologies are advancing rapidly, many research topics often require selective sequencing of genomic regions of interest. In addition, sequencing low-titre viruses is challenging, especially for coronaviruses, which are the largest RNA viruses. Prior to sequencing, enrichment of viral particles can help to significantly increase target sequence information as well as avoid large sequencing efforts and, consequently, can increase sensitivity and reduce sequencing costs. Targeting nucleic ac...
Source: Virus Research - December 15, 2023 Category: Virology Authors: Andres Ceballos-Garzon Sophie Comtet-Marre Pierre Peyret Source Type: research
Pathological and virological findings of type I interferon receptor knockout mice upon experimental infection with Heartland virus
Virus Res. 2023 Dec 12:199301. doi: 10.1016/j.virusres.2023.199301. Online ahead of print.ABSTRACTHeartland virus (HRTV) causes generalized symptoms, severe shock, and multiple organ failure. We previously reported that interferon-α/β receptor knockout (IFNAR-/-) mice infected intraperitoneally with 1 × 107 tissue culture-infective dose (TCID50) of HRTV died, while those subcutaneously infected with the same dose of HRTV did not. The pathophysiology of IFNAR-/- mice infected with HRTV and the mechanism underlying the difference in disease severity, which depends on HRTV infection route, were analyzed in this study. The ...
Source: Virus Research - December 14, 2023 Category: Virology Authors: Hikaru Fujii Shuetsu Fukushi Tomoki Yoshikawa Noriyo Nagata Satoshi Taniguchi Masayuki Shimojima Souichi Yamada Hideki Tani Akihiko Uda Takahiro Maeki Shizuko Harada Takeshi Kurosu Chang Kweng Lim Eri Nakayama Mutsuyo Takayama-Ito Shumpei Watanabe Hideki Source Type: research
Pathological and virological findings of type I interferon receptor knockout mice upon experimental infection with Heartland virus
Virus Res. 2023 Dec 12:199301. doi: 10.1016/j.virusres.2023.199301. Online ahead of print.ABSTRACTHeartland virus (HRTV) causes generalized symptoms, severe shock, and multiple organ failure. We previously reported that interferon-α/β receptor knockout (IFNAR-/-) mice infected intraperitoneally with 1 × 107 tissue culture-infective dose (TCID50) of HRTV died, while those subcutaneously infected with the same dose of HRTV did not. The pathophysiology of IFNAR-/- mice infected with HRTV and the mechanism underlying the difference in disease severity, which depends on HRTV infection route, were analyzed in this study. The ...
Source: Virus Research - December 14, 2023 Category: Virology Authors: Hikaru Fujii Shuetsu Fukushi Tomoki Yoshikawa Noriyo Nagata Satoshi Taniguchi Masayuki Shimojima Souichi Yamada Hideki Tani Akihiko Uda Takahiro Maeki Shizuko Harada Takeshi Kurosu Chang Kweng Lim Eri Nakayama Mutsuyo Takayama-Ito Shumpei Watanabe Hideki Source Type: research
