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Cancer: Bone Cancers

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Total 366 results found since Jan 2013.

GSE93677 Identification of a Novel SYK/c-MYC/MALAT1 Signaling Pathway and Its Potential Therapeutic Value in Ewing Sarcoma
Contributors : Haibo Sun ; De-Chen Lin ; Qi Cao ; Brenden Pang ; David-Hayon Gae ; Victor K Lee ; Huey J Lim ; Ngan Doan ; Jonathan W Said ; Sigal Gery ; Anand Mayakonda ; Charles Forscher ; Jeffery W Tyner ; Phillip H KoefflerSeries Type : Expression profiling by arrayOrganism : Homo sapiensEwing Sarcoma (EWS) is a devastating soft tissue sarcoma affecting predominantly young individuals. Tyrosine kinases (TKs) and associated pathways are continuously activated in many malignancies including EWS; these enzymes provide candidate therapeutic targets. Using two high-throughput screens with EWS cell lines (a siRNA library and...
Source: GEO: Gene Expression Omnibus - January 18, 2017 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Effects of long non-coding RNA SPRY4-IT1 on osteosarcoma cell biological behavior.
In conclusion, these data suggest that SPRY4-IT1 could be an oncogene for OS, and may be served as a candidate target for new therapies in human OS. PMID: 28078006 [PubMed]
Source: American Journal of Translational Research - January 13, 2017 Category: Research Tags: Am J Transl Res Source Type: research

Bufalin Inhibits Proliferation and Induces Apoptosis in Osteosarcoma Cells by Downregulating MicroRNA-221.
Authors: Zhang J, Sha J, Zhou Y, Han K, Wang Y, Su Y, Yin X, Hu H, Yao Y Abstract Bufalin, a major component of the Chinese medicine ChanSu, which is prepared from the skin and parotid venom glands of toads, has shown cytotoxicity in several malignant tumors. Here, we reported that bufalin inhibited proliferation and induced mitochondria-dependent apoptosis in U-2OS and Saos-2 osteosarcoma cells with intracellular reactive oxygen species (ROS) production. By microRNA (miR) array analysis and quantitative reverse transcription polymerase chain reaction, we found that miR-221 was downregulated after treatment with bu...
Source: Evidence-based Complementary and Alternative Medicine - January 13, 2017 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

Elevated expression of serine/threonine phosphatase type 5 correlates with malignant proliferation in human osteosarcoma.
Abstract Osteosarcoma is the most common primary malignant bone tumor in adolescents and young adults. However, the involvement of serine/threonine phosphatase type 5 (PP5) in osteosarcoma remains unclear. The aim of this study was to evaluate the functional role of PP5 in osteosarcoma cells. Firstly, we found that PP5 is widely expressed in several human osteosarcoma cell lines. Then we used lentivirus-delivered siRNA to silence PP5 expression in Saos-2 and U2OS cell lines. Knockdown of endogenous PP5 expression by shRNA-expressing lentivirus significantly decreased the viability and proliferation of the osteosar...
Source: Acta Biochim Pol - January 12, 2017 Category: Biochemistry Authors: Han K, Gan Z, Lin S, Hu H, Shen Z, Min D Tags: Acta Biochim Pol Source Type: research

Silencing LPAAT β inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro.
Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro. Int J Oncol. 2016 Dec 28;: Authors: Song L, Duan P, Gan Y, Li P, Zhao C, Xu J, Zhang Z, Zhou Q Abstract Cisplatin-resistance has become a major impediment in the medical treatment of cancers such as osteosarcoma, the most common primary malignancy of bone. Since lysophosphatidic acid acyltransferase β (LPAATβ) was reported to be critically involved in osteosarcoma, our study investigated the role of LPAATβ in human osteosarcoma with cisplatin-resistance. Expression of LPAATβ or other relevant pr...
Source: International Journal of Oncology - December 27, 2016 Category: Cancer & Oncology Authors: Song L, Duan P, Gan Y, Li P, Zhao C, Xu J, Zhang Z, Zhou Q Tags: Int J Oncol Source Type: research

Targeting specificity protein 1 transcription factor and survivin using tolfenamic acid for inhibiting Ewing sarcoma cell growth
SummaryTranscription factor Specificity protein  1 (Sp1) and its downstream target survivin (inhibitor of apoptosis protein), play major roles in the pathogenesis of various cancers. Ewing Sarcoma (ES) is a common soft tissue/bone tumor in adolescent and young adults. Overexpression of survivin is also linked to the aggressiveness and poor prog nosis of ES. Small molecule Tolfenamic acid (TA) inhibits Sp1 and survivin in cancer cells. In this investigation, we demonstrate a strategy to target Sp1 and survivin using TA and positive control Mithramycin A (Mit) to inhibit ES cell growth. Knock down of Sp1 using small interf...
Source: Investigational New Drugs - December 25, 2016 Category: Drugs & Pharmacology Source Type: research

Inhibition of casein kinase  2 prevents growth of human osteosarcoma.
We examined the expression of CK2α and CK2β by western blot analysis, and performed WST-1 assays using CK2α and CK2β siRNA or CX-4945. Flow cytometry and western blot analyses were performed to evaluate apoptotic cell death. Xenograft models were used to examine the effect of CX-4945 in vivo. Western blot analysis revealed upregulation of CK2α and CK2β in human osteosarcoma cell lines compared with human osteoblast cells or mesenchymal stem cells. WST assay showed that knockdown of CK2α or CK2β by siRNA inhibited the proliferation of human osteosarcoma cells. Treatment with 3 µM of CX-4945 inhibited osteosarcoma...
Source: Oncology Reports - December 15, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

LDHB may be a significant predictor of poor prognosis in osteosarcoma.
Authors: Li C, Chen Y, Bai P, Wang J, Liu Z, Wang T, Cai Q Abstract Osteosarcoma is the most common primary malignant bone tumor in children and young adults. Lactate dehydrogenase (LDH) is considered as the key glycolytic enzyme and involved in tumor initiation and metabolism. Here, we firstly found that LDHB was highly expressed in osteosarcoma cell lines. Expression profiling indicated that LDHB mRNA was elevated in osteosarcoma tissues with metastasis versus without metastasis, and LDHB high expression predicted a poor prognosis in patients. After LDHB knockdown by siRNA transfection, cell growth and proliferat...
Source: American Journal of Translational Research - December 3, 2016 Category: Research Tags: Am J Transl Res Source Type: research

Elevated expression of matrix metalloproteinase-3 in human osteosarcoma and its association with tumor metastasis.
CONCLUSION: Our results demonstrated that MMP-3 expression is deregulated in osteosarcomas and this potentially contributes to metastasis and might be a promising marker for the prognosis and therapy of metastatic osteosarcoma. PMID: 27837634 [PubMed - in process]
Source: Journal of B.U.ON. - November 14, 2016 Category: Cancer & Oncology Tags: J BUON Source Type: research

Long non-coding RNA PVT1 promotes osteosarcoma development by acting as a molecular sponge to regulate miR-195.
In this study, the qRT-PCR results indicated that the lncRNA PVT1 is overexpressed in osteosarcoma and decreased the survival rate of osteosarcoma patients. MTT and clonal colony formation assays were used to detect the effect of PVT1 on proliferation, and flow cytometry was performed to assess apoptosis and the cell cycle. A Transwell assay was used to analyze migration and invasion. The results revealed that silencing PVT1 by siRNA inhibited proliferation, migration and invasion and promoted apoptosis and cell cycle arrest in osteosarcoma cells. Furthermore, a gene microarray was used to screen differentially expressed m...
Source: Oncotarget - November 6, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Down-Regulation of MicroRNA-133b Suppresses Apoptosis of Lens Epithelial Cell by Up-Regulating BCL2L2 in Age-Related Cataracts.
CONCLUSIONS BCL2L2 was the virtual target of miR-133b, and we found a negative regulatory relationship between miR-133b and BCL2L2. MiR-133b and BCL2L2 interfered with the viability and apoptosis of cells. PMID: 27802259 [PubMed - in process]
Source: Medical Science Monitor - November 2, 2016 Category: Research Tags: Med Sci Monit Source Type: research

Targeting miR-29 induces apoptosis of osteosarcoma MG-63 cells via regulation of TGF- β1/PUMA signal.
CONCLUSIONS: Targeting miR-29 exhibits significant in vivo and in vitro anti-tumor activities in OS through a novel mechanism resulting in inhibition of TGF-β1 expression and inducing PUMA expression. PMID: 27649654 [PubMed - as supplied by publisher]
Source: European Review for Medical and Pharmacological Sciences - September 23, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Circadian clock components ROR α and Bmal1 mediate the anti-proliferative effect of MLN4924 in osteosarcoma cells.
In this study, we demonstrate that MLN4924 suppresses osteosarcoma cell proliferation by inducing G2/M cell cycle arrest and apoptosis. Our results indicate that MLN4924 stabilizes the retinoid orphan nuclear receptor alpha (RORα) by decreasing its ubiquitination. RNA interference of RORα attenuates the anti-proliferative effect of MLN4924 in U2OS osteosarcoma cells. MLN4924 up-regulates the expression of p21 and Bmal1, two transcriptional targets of RORα. However, p21 plays a minimal role in the anti-proliferative effect of MLN4924 in U2OS osteosarcoma cells. In contrast, Bmal1 suppression by siRNA attenuates the anti-...
Source: Oncotarget - September 10, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Downregulation of DNA-PKcs suppresses P-gp expression via inhibition of the Akt/NF- κB pathway in CD133-positive osteosarcoma MG-63 cells.
In this study, CD133+ and CD133‑negative (CD133‑) MG‑63 cells were isolated by magnetic activated cell sorting (MACS). We verified that CD133+ MG‑63 cells were more resistant to cisplatin (CDDP) than CD133‑ MG‑63 cells. DNA‑dependent protein kinase catalytic subunit (DNA‑PKcs) and P‑glycoprotein (P‑gp) were expressed at higher levels in the CD133+ MG‑63 cells compared with those levels in the CD133‑ MG‑63 cells, whereas downregulation of DNA‑PKcs by small interfering RNA (siRNA) decreased chemoresistance to CDDP and P‑gp expression at the mRNA and protein levels in these cells. This indi...
Source: Oncology Reports - August 9, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Hypoxic Proliferation of Osteosarcoma Cells Depends on Arginase II
Conclusions: These data support our hypothesis that hypoxia increases proliferation of osteosarcoma cells in an arginase II-dependent manner. We speculate that arginase II may represent a therapeutic target in osteosarcoma. Cell Physiol Biochem 2016;39:802-813
Source: Cellular Physiology and Biochemistry - July 29, 2016 Category: Cytology Source Type: research