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The uptake exploration of 68Ga-labeled NGR in well-differentiated hepatocellular carcinoma xenografts: Indication for the new clinical translational of a tracer based on NGR.
Authors: Gao Y, Wang Z, Ma X, Ma W, Zhao M, Fu T, Li G, Wang S, Wang Z, Yang W, Kang F, Wang J Abstract 18F-FDG has low uptake and poor diagnostic efficiency in hepatocellular carcinoma (HCC), particularly in well-differentiated HCC. The NGR peptide selectively targets CD13, which is overexpressed in many types of tumor cells as well as neovasculature cells. In the present study, we aimed to evaluate the feasibility of utilizing 68Ga-NGR to image CD13-positive well-differentiated HCC xenografts. The in vitro cellular uptake, in vivo micro-PET/CT imaging and biodistribution studies of 68Ga-NGR and 18F-FDG ...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

The antitumor effects of arsenic trioxide in mantle cell lymphoma via targeting Wnt/ β‑catenin pathway and DNA methyltransferase-1.
In this study, ATO was shown to promote apoptosis and to inhibit cell viability in MCL cell lines, whereas, the expression of DNA methyltransferase-1 (DNMT-1), β‑catenin and the downstream molecules of Wnt/β‑catenin pathway such as c‑myc, cyclin D1 and MMP7 were all decreased in a dose-dependent manner with ATO. ATO also attenuated upregulation of β‑catenin after LiCl stimulation and provided synergistic effect with 5-azacytidine (5-azaC) on the DNMT-1 inhibition. The results indicated that ATO may suppress MCL by targeting Wnt/β‑catenin pathway and DNMT-1. These findings may guide drug u...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Screening and identification of key biomarkers in hepatocellular carcinoma: Evidence from bioinformatic analysis.
In conclusion, DEGs and hub genes identified in the present study help us understand the molecular mechanisms underlying the carcinogenesis and progression of HCC, and provide candidate targets for diagnosis and treatment of HCC. PMID: 28901457 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miR106b regulates retinoblastoma Y79 cells through Runx3.
Authors: Yang G, Fu Y, Zhang L, Lu X, Li Q Abstract MicroRNAs are increasingly recognized as important regulators of cancer. The aim of the present study was to investigate the role of miR-106b in the regulation of Y79 retinoblastoma. Y79 cells were transfected with antisense oligonucleotides (ASO) against miR-106b (ASO-miR-106b) or ASO-control. After transfection, the levels of miR-106b were monitored with real-time PCR (RT-PCR). The effects of ASO-miR-106b transfection on cell viability was evaluated by Cell Counting Kit-8 (CCK-8) analysis at 24, 48 and 72 h after transfection. Subsequently, the cells were s...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

1H-NMR-based metabolic profiling of a colorectal cancer CT-26 lung metastasis model in mice.
Authors: Li Y, Wang C, Li D, Deng P, Shao X, Hu J, Liu C, Jie H, Lin Y, Li Z, Qian X, Zhang H, Zhao Y Abstract Lung metastasis is an important cause for the low 5-year survival rate of colorectal cancer patients. Understanding the metabolic profile of lung metastasis of colorectal cancer is important for developing molecular diagnostic and therapeutic approaches. We carried out the metabonomic profiling of lung tissue samples on a mouse lung metastasis model of colorectal cancer using 1H-nuclear magnetic resonance (1H-NMR). The lung tissues of mice were collected at different intervals after marine colon cancer cel...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miR-133b acts as a tumor suppressor and negatively regulates ATP citrate lyase via PPAR γ in gastric cancer.
miR-133b acts as a tumor suppressor and negatively regulates ATP citrate lyase via PPARγ in gastric cancer. Oncol Rep. 2017 Sep 07;: Authors: Cheng Y, Jia B, Wang Y, Wan S Abstract MicroRNAs (miRNAs/miRs) are a class of small noncoding RNAs that negatively regulate protein expression by binding to protein-coding mRNAs and suppressing translation. Accumulating evidence suggests that miRNAs are involved in the development and progression of cancer by regulating cancer metabolism. Meanwhile, the cytosolic enzyme ATP citrate lyase (ACLY) is a promising target in the prevention and treatment...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

TLR5/7-mediated PI3K activation triggers epithelial-mesenchymal transition of ovarian cancer cells through WAVE3-dependent mesothelin or OCT4/SOX2 expression.
In this study, we examined the role of TLR5 and TLR7 in the metastasis of ovarian cancer through the induction of mesothelin/CA125 expression and investigated its underlying mechanism. TLR5 agonist (flagellin) and TLR7 agonist (imiquimod) upregulated mesenchymal phenotypes and produced epithelial-mesenchymal transition (EMT)-related cytokines in the SKOV3 cells; however, TLR7 expressing CaOV3 cells had no response to the specific ligand, imiquimod, for enhancing its EMT processes. Stimulation of the SKOV3 cells with flagellin or imiquimod activated Wiskott-Aldrich syndrome protein verprolin-homologous 3 (WAVE3) and me...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inositol hexaphosphate hydrolysate competitively binds to AKT to inhibit the proliferation of colon carcinoma.
Authors: Chen C, Yang F, Liu C, Cui L, Fu M, Song Y Abstract Phytate, myto-inositol 1,2,3,4,5,6 hexaphosphate (IP6), is recognized as an anti-nutrition phytochemical for decades. Recently, numerous studies have indicated that IP6 and its hydrolysates could suppress colon oncogenesis. However, very little is known concerning the mechanism of IP6 hydrolysates in regulating colon oncogenesis. The aim of the present study was to identify the underlying relationship between IP6 hydrolysates and colon cancer. Three types of human colorectal cancer cells were utilized in the present study. The proliferation inhibitio...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibition of miR-23a increases the sensitivity of lung cancer stem cells to erlotinib through PTEN/PI3K/Akt pathway.
Authors: Han Z, Zhou X, Li S, Qin Y, Chen Y, Liu H Abstract Epidermal growth factor receptor-targeted tyrosine kinase inhibitors (EGFR-TKIs) have become first-line drugs used for non-small cell lung cancer (NSCLC) treatment. However, drug resistance to EGFR-TKIs will be developed inevitably due to the repeated use of these drugs. In the present study, we isolated cancer stem cells (CSCs) from the PC9 NSCLC cell line. We then observed that the PC9 CSCs showed significant resistance to erlotinib compared with the PC9 non-CSCs. Erlotinib failed to suppress the phosphorylation of PI3K and AKT in PC9 CSCs, although the ...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Interaction with neutrophils promotes gastric cancer cell migration and invasion by inducing epithelial-mesenchymal transition.
Authors: Zhang W, Gu J, Chen J, Zhang P, Ji R, Qian H, Xu W, Zhang X Abstract Emerging evidence has revealed that neutrophils have phenotypic and functional plasticity. Neutrophils could be polarized towards a pro-tumor phenotype by tumor-derived factors. In the present study, we investigated the role of the interaction with neutrophils on the functions of gastric cancer cells in vitro. Human promyelocytic leukemia HL-60 cells were induced to differentiate into neutrophil-like cells (HL-60N) using dimethyl sulfoxide (DMSO). Human gastric cancer cells were co-cultured with HL-60N cells or treated with...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Radioiodine-labeled anti-epidermal growth factor receptor binding bovine serum albumin-polycaprolactone for targeting imaging of glioblastoma.
In conclusion, the data showed 131I-EGFR-BSA-PCL leading radioiodine therapy for U251 and U87 cells had a good effect in vitro and in vivo. Thus, 131I-EGFR-BSA-PCL may provide a new method for glioblastoma treatment. PMID: 28901480 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of apoptosis.
Authors: Liu Z, Piao L, Zhuang M, Qiu X, Xu X, Zhang D, Liu M, Ren D Abstract NSD3 is a histone lysine methyltransferase that methylates histone H3 at lysine 36. NSD3 is located at chromosome 8p11.23, the locus that exhibits strong cancer relevance. Thus, NSD3 is likely involved in multiple human cancers. Nevertheless, its roles in human carcinogenesis remain unknown. In the present study, we demonstrated that silencing of NSD3 in osteosarcoma, the most common primary bone cancer in children and adolescents, results in a marked decrease in the number of viable cancer cells, accompanied by increases i...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Molecular iodine impairs chemoresistance mechanisms, enhances doxorubicin retention and induces downregulation of the CD44+/CD24+ and E-cadherin+/vimentin+ subpopulations in MCF-7 cells resistant to low doses of doxorubicin.
In conclusion, I2 is able to reduce the drug resistance and invasive capacity of mammary cancer cells exposed to DOX and represents an anti-chemoresistance agent with clinical potential. PMID: 28901484 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miRNA ‑148a inhibits cell growth of papillary thyroid cancer through STAT3 and PI3K/AKT signaling pathways.
miRNA‑148a inhibits cell growth of papillary thyroid cancer through STAT3 and PI3K/AKT signaling pathways. Oncol Rep. 2017 Sep 07;: Authors: Xu Y, Han YF, Zhu SJ, Dong JD, Ye B Abstract The function of miRNA‑148a in lymphatic metastases of papillary thyroid cancer and its mechanism were tested. In this investigation, miRNA‑148a expression of lymphatic metastases of papillary thyroid cancer patients was inhibited, compared with normal group. We found that miRNA‑148a overexpression was effectively reduced cell cell proliferation and metastases, and induced apoptosis of papillary thyroid cancer in...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

High pemetrexed sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase.
Authors: Kuo WT, Tu DG, Chiu LY, Sheu GT, Wu MF Abstract The chemoresistance of non-small cell lung cancer (NSCLC) that occurs in docetaxel (DOC) chemotherapy substantially decreases the survival of patients. To overcome DOC-induced chemoresistance, we established DOC-selected A549 lung cancer sublines (A549/D16 and A549/D32) and revealed that both sublines were cross-resistant to vincristine (VCR) and doxorubicin (DXR). Notably, both sublines were more sensitive to pemetrexed (PEM) than parental cells according to MTT and clonogenic assays. The expression levels of thymidylate synthase (TS) and γ-glutamyl hy...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells.
Authors: Cho IA, You SJ, Kang KR, Kim SG, Oh JS, You JS, Lee GJ, Seo YS, Kim DK, Kim CS, Lee SY, Kim JS Abstract The aim of the present study was to investigate biochanin-A-induced anticancer effects and their cellular signaling pathway in FaDu pharyngeal squamous carcinoma cells. Biochanin-A induced cell death through increased cytotoxicity of FaDu cells in a dose- and time-dependent manner. The number of cells with nucleus condensation and the apoptotic population were increased in the FaDu cells stimulated with biochanin-A for 24 h. Furthermore, extrinsic apoptotic factors such as FasL and their downstream ...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Regulation of UHRF1 by microRNA-378 modulates medulloblastoma cell proliferation and apoptosis.
Authors: Zhang ZY, Zhu B, Zhao XW, Zhan YB, Bao JJ, Zhou JQ, Zhang FJ, Yu B, Liu J, Wang YM, Bai YH, Hong J, Liu XZ Abstract A previous study revealed that ubiquitin-like with PHD and RING finger domains 1 (UHRF1) promoted cell proliferation and was a potential biomarker in medulloblastoma (MB). In the present study, we reported that miR-378 inhibited the expression of UHRF1 to affect the proliferation of MB through competitive binding to the same region of its 3'-UTR. We found that the expression of miR-378 was significantly downregulated in MB tissues and inversely correlated with the expression of UHRF1. We...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway.
In conclusion, our data revealed that harmine inhibited the proliferation and migration of SKOV-3 cells, which might be mediated by ERK/CREB pathway. These findings elucidate that harmine may act as a potential therapeutic drug for ovarian cancer treatment. PMID: 28901502 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

α7 nicotinic acetylcholine receptor in tumor-associated macrophages inhibits colorectal cancer metastasis through the JAK2/STAT3 signaling pathway.
α7 nicotinic acetylcholine receptor in tumor-associated macrophages inhibits colorectal cancer metastasis through the JAK2/STAT3 signaling pathway. Oncol Rep. 2017 Sep 04;: Authors: Fei R, Zhang Y, Wang S, Xiang T, Chen W Abstract Considerable evidence has implied that α7 nicotinic receptor subtypes play an important role in chronic inflammatory and neuropathic pain signaling. The aim of the present study was to determine the role of endogenous α7nAChR signaling in tumor-associated macrophages (TAMs) in human colorectal cancer (CRC) metastasis and prognosis. α7nAChR expression i...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Multimodal actions of the phytochemical sulforaphane suppress both AR and AR-V7 in 22Rv1 cells: Advocating a potent pharmaceutical combination against castration-resistant prostate cancer.
Authors: Khurana N, Kim H, Chandra PK, Talwar S, Sharma P, Abdel-Mageed AB, Sikka SC, Mondal D Abstract Prostate cancer (PCa) cells expressing full-length androgen receptor (AR-FL) are susceptible to androgen deprivation therapy (ADT). However, outgrowth of castration-resistant prostate cancer (CRPC) can occur due to the expression of constitutively active (ligand-independent) AR splice variants, particularly AR-V7. We previously demonstrated that sulforaphane (SFN), an isothiocyanate phytochemical, can decrease AR-FL levels in the PCa cell lines, LNCaP and C4-2B. Here, we examined the efficacy of SFN in targeting ...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Cx32 inhibits TNF α-induced extrinsic apoptosis with and without EGFR suppression.
Cx32 inhibits TNFα-induced extrinsic apoptosis with and without EGFR suppression. Oncol Rep. 2017 Sep 07;: Authors: Lai Y, Tao L, Zhao Y, Zhang X, Sun X, Wang Q, Xu C Abstract Tumor necrosis factor α (TNFα) and TNF-related apoptosis-inducing ligand (TRAIL) can trigger the extrinsic apoptosis pathway. Our previous study indicated that connexin32 (Cx32) inhibited streptonigrin-induced intrinsic apoptosis via the epidermal growth factor receptor (EGFR) pathway. However, whether Cx32 can exert effects on the extrinsic apoptosis pathway through EGFR signaling remains unclear. In the presen...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Salidroside, a scavenger of ROS, enhances the radioprotective effect of Ex-RAD ® via a p53-dependent apoptotic pathway.
Salidroside, a scavenger of ROS, enhances the radioprotective effect of Ex-RAD® via a p53-dependent apoptotic pathway. Oncol Rep. 2017 Sep 06;: Authors: Feng T, Wang L, Zhou N, Liu C, Cui J, Wu R, Jing J, Zhang S, Chen H, Wang S Abstract Salidroside (Sal), the predominant component of a Chinese medicinal herb, Rhodiola rosea L., has become an attractive bioagent due to its significant anti-radiation, antioxidant and immune adjustment effects. We explored the radioprotective effect of Sal to ascertain whether it could enhance the anti-radiation effect of ON 01210.Na (Ex-RAD®) in vivo and in...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Upregulation of MUC1 by its novel activator 14-3-3 ζ promotes tumor invasion and indicates poor prognosis in lung adenocarcinoma.
In conclusion, we provide novel evidence that 14-3-3ζ regulates MUC1 through MUC1/NF-κB feedback loop. 14-3-3ζ and MUC1 is a promising prognostic biomarker for lung cancer patients and therapeutic targeting of 14-3-3ζ and MUC1 may be a potential treatment option for patients with LAC. PMID: 28901525 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Hypoxia promotes migration/invasion and glycolysis in head and neck squamous cell carcinoma via an HIF-1 α-MTDH loop.
Hypoxia promotes migration/invasion and glycolysis in head and neck squamous cell carcinoma via an HIF-1α-MTDH loop. Oncol Rep. 2017 Sep 07;: Authors: Zhu G, Peng F, Gong W, She L, Wei M, Tan H, Chen C, Zhang D, Li G, Huang D, Zhang X, Liu Y Abstract Hypoxia is a hallmark of progressive cancer. Hypoxic cancer cells trigger glycolysis in response to a decreased O2 supply to meet metabolic and bioenergetic demands. Meanwhile, these responses to hypoxia and alterations of the microenvironment promote cancer cell metastasis by increasing transcription of hypoxia-inducible factor (HIF)-regulated genes...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Identification of hub genes, key miRNAs and potential molecular mechanisms of colorectal cancer.
Authors: Wu S, Wu F, Jiang Z Abstract Colorectal cancer (CRC) is the most common cancer of the digestive system. The aim of the present study was to identify the potential biomarkers and uncover the underlying mechanisms. The gene and miRNA expression profiles were obtained from GEO database. The differentially expressed genes (DEGs) and miRNAs (DE miRNAs) were identified by GEO2R. The gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed by KOBAS 3.0. The protein-protein interaction (PPI) network and miRNA-gene network were constructed by Cytoscape softwar...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Smad4 re-expression increases the sensitivity to parthenolide in colorectal cancer.
Authors: Li X, Yang H, Ke J, Liu B, Lv X, Li X, Zhang Y Abstract Parthenolide (PT), a sesquiterpene lactone extracted from the plant feverfew, has been demonstrated to have anti-inflammatory and anticancer properties. Although PT has been revealed to markedly inhibit colorectal cancer cell proliferation, the inhibitory effects decrease with administration time. These findings revealed that colorectal cancer cells develop resistance to PT. However, the underlying mechanism is unclear. In the present study we observed significantly low expression of Smad4 in 3 PT-resistant cell lines (HCT‑116/PT, HT-29/PT...
Source: Oncology Reports - September 14, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Knockdown of c ‑Myc activates Fas-mediated apoptosis and sensitizes A549 cells to radiation.
In this study, we established radiation-resistant A549 cell model (A549/R), and investigated the roles of c‑Myc and Fas in radiation-induced cytotoxicity of A549 cells. Apoptosis detection showed that there were fewer apoptotic cells in A549/R cells treated with radiation than in A549 cells. Western blotting results demonstrated the inverse expression pattern of c‑Myc and Fas in A549 and A549/R cells. Suppression of c‑Myc expression by small interfering RNA (siRNA) displayed enhancement of Fas-mediated apoptosis in A549/R cells, accompanying a significant decrease of Bid, Bcl‑2, pro‑caspase‑8, -9 and -3 and inc...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

WRAP53 β, survivin and p16INK4a expression as potential predictors of radiotherapy/chemoradiotherapy response in T2N0-T3N0 glottic laryngeal cancer.
WRAP53β, survivin and p16INK4a expression as potential predictors of radiotherapy/chemoradiotherapy response in T2N0-T3N0 glottic laryngeal cancer. Oncol Rep. 2017 Aug 11;: Authors: Tiefenböck-Hansson K, Haapaniemi A, Farnebo L, Palmgren B, Tarkkanen J, Farnebo M, Munck-Wikland E, Mäkitie A, Garvin S, Roberg K Abstract The current treatment recommendation for T2-3N0M0 glottic squamous cell carcinoma (SCC) in the Nordic countries comprises of radiotherapy (RT) and chemoradiotherapy (CRT). Tumor radiosensitivity varies and another option is primary surgical treatment, which ...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Preclinical evaluation of cisplatin-incorporated bio-nanocapsules as chemo-radiotherapy for human hepatocellular carcinoma.
Authors: Shin SH, Park SS, Lee KJ, Ju EJ, Park J, Ko EJ, Jung J, Kuroda S, Hong SM, Hwang JJ, Lee JS, Song SY, Jeong SY, Choi EK Abstract The incidence of hepatocellular carcinoma (HCC) has continued to increase worldwide, and advanced HCC is difficult to treat using the currently available therapeutics. Chemoradiotherapy with cisplatin (cis-diamminedichloroplatinum, CDDP) is expected to confer a curative benefit on HCC patients; however, its application is limited due to side-effects such as acute nephrotoxicity as well as the conventionally limited application of chemoradiotherapy for HCC. For the practical appli...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miR-187 inhibits the growth of cervical cancer cells by targeting FGF9.
In this study, we found that miR-187 level was decreased in cervical cancer tissues and cell lines. Patients with low level of miR-187 had significantly decreased rate of overall survival (OS) and progression-free survival (DFS). miR-187 overexpression inhibited proliferation and promoted apoptosis of cervical cancer cells, whereas miR-187 knockdown promoted proliferation and inhibited apoptosis of cervical cancer cells. Forced expression of miR-187 inhibited the subcutaneous growth of cervical cancer cells in nude mice. Furthermore, FGF9 was found to be the downstream target of miR-187 in cervical cancer cells. Importantl...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

c ‑Myc promotes cholangiocarcinoma cells to overcome contact inhibition via the mTOR pathway.
c‑Myc promotes cholangiocarcinoma cells to overcome contact inhibition via the mTOR pathway. Oncol Rep. 2017 Aug 22;: Authors: Luo G, Li B, Duan C, Cheng Y, Xiao B, Yao F, Wei M, Tao Q, Feng C, Xia X, Zhou H, Zhao X, Dai R Abstract The loss of contact inhibition is a hallmark of a wide range of human cancer cells. Yet, the precise mechanism behind this process is not fully understood. c‑Myc plays a pivotal role in carcinogenesis, but its involvement in regulating contact inhibition has not been explored to date. Here, we report that c‑Myc plays an important role in abrogating contact inhibition i...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/ β-catenin signaling pathway.
This study was performed to address this gap in knowledge. GNL3 expression was upregulated in colon cancer tissue specimens and correlated with tumor differentiation, invasion and metastasis. GNL3 overexpression promoted cell proliferation, invasion, migration and the epithelial-mesenchymal transition (EMT) in colon cancer cells. Moreover, inhibition of the EMT and the Wnt/β‑catenin signaling pathway induced by GNL3 knockdown was partially reversed by lithium chloride (LiCl). Based on these data, GNL3 promotes the EMT in colon cancer by activating the Wnt/β‑catenin signaling pathway. In summary, GNL3 is upreg...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Screening and identification of potential biomarkers in triple-negative breast cancer by integrated analysis.
Authors: Guo J, Gong G, Zhang B Abstract Triple-negative breast cancer (TNBC) has attracted great attention due to its unique biology, poor prognosis, and aggressiveness. TNBC patients are more likely to suffer from metastasis. We screened and identified the TNBC-specific genes as potential biomarkers. A total of 167 breast cancer samples (45 TNBC and 122 non-TNBC) were used in the integrated analysis. Gene expression microarrays were used to screen the differentially expressed genes. We identified 65 core DEGs. According to the GO and KEGG analysis, the gene function enrichment in TNBC was revealed,...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Downregulation of microRNA-15a suppresses the proliferation and invasion of renal cell carcinoma via direct targeting of eIF4E.
Authors: Li G, Chong T, Xiang X, Yang J, Li H Abstract The downregulation of microRNA-15a has been reported in several human tumors. However, its expression and functional importance in renal cell carcinoma (RCC) remain unknown. The aim of the present study was to investigate its expression, biological functions and underlying mechanisms in RCC tumorigenesis. The expression levels of miR-15a were examined by qRT-PCR in 40 RCC specimens and adjacent‑paired normal tissues. Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry and Transwell assays were used to explore the potential influence of miR-15a ...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Functional expression, characterization, and application of human S100B.
In this study, we prepared human S100B protein and its monoclonal antibodies. Human S100B protein was expressed in Escherichia coli, successfully purified by diethylaminoethyl cellulose anion-exchange chromatography, and then identified by western blot analysis. Monoclonal antibodies (mAbs) were produced by the standard hybridoma method and validated by enzyme-linked immunosorbent assay and western blot analysis. The prepared human S100B protein and its mAbs demonstrated potential biological activities. The KD of one mAb is approximately 4.72x10-8 mol/l, and its cross reactivity is low with human S100A4, mouse S100A4,...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Long non-coding RNAs on the stage of cervical cancer (Review).
In conclusion, lncRNAs may be useful as exact treatment targets and diagnostic biomarkers for improving therapies in cervical cancer patients and lncRNAs may contribute to effective diagnosis and treatment methods for cervical cancer. PMID: 28849103 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Killing effect of methionine enkephalin on melanoma in vivo and in vitro.
In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. Therefore, we posit that MENK should be investigated, not only as a primary therapy for melanoma, but also as an adjuvant therapy in combination with chemotherapies. PMID: 28849104 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells.
Authors: Wang J, Li Z, Zuo C, Xie Q, Li H, Jia J, Zhen Z, Qi R, Li Z, Liu D, Sun B Abstract In recent years it was found that the synthesis and biological activity of ribosomes are closely associated with tumor cell growth, tumorigenesis, and malignant transformation. However, the role of regulator of ribosome synthesis 1 (RRS1) in hepatocellular carcinoma (HCC) has not yet been reported. In the present study, we aimed to examine the potential role of RRS1 in tumor cell growth by using a lentivirus-mediated RNA interference (RNAi) system in the HCC cell line SMMC-7721 in vitro. Compared with that of the n...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Scutellaria barbata D. Don inhibits 5-fluorouracil resistance in colorectal cancer by regulating PI3K/AKT pathway.
Authors: Lin J, Feng J, Yang H, Yan Z, Li Q, Wei L, Lai Z, Jin Y, Peng J Abstract 5-Fluorouracil (5-FU) resistance or multidrug resistance (MDR) has become a major obstacle in clinical treatment of cancers including colorectal cancer (CRC). Aberrant activation of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway may lead to unlimited growth and chemoresistance in CRC cells, which thus could be a promising therapeutic target. As a long-term used traditional Chinese folk-medicine, Scutellaria barbata D. Don (SB) processes specific anticancer activity, but its activity against cancer chemoresist...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K ‑Ras‑p110α interaction.
Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K‑Ras‑p110α interaction. Oncol Rep. 2017 Aug 22;: Authors: Gao M, Guo KM, Wei YM, Ma MM, Cai JR, Xia TT, Ye QJ Abstract Aspirin has been confirmed as an effective antitumor drug in various cancers. However, the relationship between aspirin and uterine leiomyoma is still underexplored. Here, we explored the effects of aspirin on human uterine leiomyoma cells and provide insights into the underlying mechanisms. Cell Counting Kit-8 (CCK-8) and flow cytometry analysis showed that aspirin treatment inhibited c...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miR-217 targeting DKK1 promotes cancer stem cell properties via activation of the Wnt signaling pathway in hepatocellular carcinoma.
Authors: Jiang C, Yu M, Xie X, Huang G, Peng Y, Ren D, Lin M, Liu B, Liu M, Wang W, Kuang M Abstract Hepatocellular carcinoma (HCC) is one of the most common malignancies and exhibits heterogeneity in terms of clinical outcomes and biological activities. Emerging evidence has demonstrated that cancer stem cells (CSCs) play important roles in the tumorigenesis and progression of HCC. However, the molecular mechanisms underlying the stemness maintenance of CSCs remain largely unknown. In the present study, through real-time PCR, western blotting, luciferase assays, RNA immunoprecipitation, and in vitro and in&nb...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

STAT3 inhibition by WP1066 suppresses the growth and invasiveness of bladder cancer cells.
We examined whether it could be a novel therapeutic target for bladder cancer using the STAT3 inhibitor WP1066. In T24 and UMUC-3 bladder cancer cells, 5 µM WP1066 prevented the phosphorylation of STAT3 and 2.5 µM WP1066 decreased cell survival and proliferation significantly (P
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA-144-3p suppresses tumor growth and angiogenesis by targeting SGK3 in hepatocellular carcinoma.
Authors: Wu M, Huang C, Huang X, Liang R, Feng Y, Luo X Abstract In our previous studies, the Illumine Soledad massively parallel signature sequencing of miRNomes in non‑tumor and hepatocellular carcinoma (HCC) tissues revealed that microRNA (miR)-144-3p was significantly downregulated in HCC, but its role in HCC development, especially angiogenesis, remains unclear. In this investigation, we found recovering miR‑144‑3p expression can significantly suppress the growth, migration and induced angiogenic capacity of HCC cells through both in vivo and in vitro experiments. Moreover, clinical correlation anal...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

EGFR gene status predicts response and survival benefit in a preclinical gastric cancer trial treating patient ‑derived xenografts with cetuximab.
EGFR gene status predicts response and survival benefit in a preclinical gastric cancer trial treating patient‑derived xenografts with cetuximab. Oncol Rep. 2017 Aug 14;: Authors: Wang X, Fu R, Hu Y, Du H, Li S, Li Z, Liu Y, Li Q, Zhang L, Ji J Abstract Cetuximab has been evaluated as a first-line treatment with conflicting results. The aim of the present study was to investigate the relationship between epidermal growth factor receptor (EGFR) status, and response and survival benefit following cetuximab treatment in gastric cancer (GC). Using 20 patient-derived GC xenograft (PDX) models, the mi...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

ABT-737 potentiates cisplatin-induced apoptosis in human osteosarcoma cells via the mitochondrial apoptotic pathway.
Authors: Zhang F, Yu X, Liu X, Zhou T, Nie T, Cheng M, Liu H, Dai M, Zhang B Abstract ABT-737 is a BH-3 mimetic that inhibits Bcl-2 and induces apoptosis of cancer cells, which has potential for anticancer therapies. Studies have shown that Bcl-2 expression in human osteosarcoma (OS) cells plays a significant role in tumor progression; however, its effects on OS cell apoptosis are still unknown. Therefore, we examined whether ABT-737 was effective in eliminating human U-2OS cells, either alone or in combination with the chemotherapy drug cisplatin [cis-diamminedichloroplatinum (II); DDP]. Furthermore, we studi...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Zhi Zhen Fang formula reverses Hedgehog pathway mediated multidrug resistance in colorectal cancer.
This study provided the first direct evidence demonstrating ZZR can attenuate MDR by repressing Hedgehog signaling in human CRC. PMID: 28849164 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Combined analysis of ChIP-seq and gene microarray datasets identify the E2-mediated genes in ER α-dependent manner in osteosarcoma.
Combined analysis of ChIP-seq and gene microarray datasets identify the E2-mediated genes in ERα-dependent manner in osteosarcoma. Oncol Rep. 2017 Aug 22;: Authors: Tian K, Qi W, Yan Q, Zhang F, Song D, Zhang H, Lv M Abstract Osteosarcoma is a common bone tumor which is affected by E2, the most representative estrogen. Gene regulation function of E2 is highly dependent on estrogen receptor. The purpose of this study was to explore the gene regulation patterns of E2 through estrogen receptor α (ESR1) in osteosarcoma based on the combined analysis of ChIP-seq and gene microarray. All of the d...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Silencing Aurora A leads to re-sensitization of breast cancer cells to Taxol through downregulation of SRC-mediated ERK and mTOR pathways.
Authors: Li Y, Zhou W, Tang K, Chen X, Feng Z, Chen J Abstract While Taxol has been reported to improve the clinical survival of breast cancer patients, subsequently developed drug-resistance of the cancer cells limits its final efficacy and applications. Previous studies suggested that Aurora A is involved in the development of the Taxol-resistance of breast cancer. We established Taxol-resistant breast cancer MCF-7/T cells and xenograft models to explore the role of Aurora A in Taxol resistant ER-positive breast cancer. Compared with their parental MCF-7/C cells, the Taxol-resistant MCF-7/T cells exhibi...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

FTO expression is associated with the occurrence of gastric cancer and prognosis.
Authors: Xu D, Shao W, Jiang Y, Wang X, Liu Y, Liu X Abstract Fat mass and obesity associated (FTO) is a protein-coding gene. FTO gene is an obesity related gene, also known as the obesity gene. It has been reported previously that FTO is associated with a variety of malignant cancers, such as breast, thyroid and endometrial cancer. The aim of the present study was investigate the FTO expression of human gastric cancer and to investigate its clinical value. FTO expression was determined by immunohistochemical analysis with tissue microarrays in GC tissues and corresponding adjacent non-tumor tissues. Moreover, the ...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA-218 promotes cisplatin resistance in oral cancer via the PPP2R5A/Wnt signaling pathway.
Authors: Zhuang Z, Hu F, Hu J, Wang C, Hou J, Yu Z, Wang TT, Liu X, Huang H Abstract Accumulating data suggest that microRNAs (miRNAs) play a pivotal role in the regulation of tumor cell sensitivity to chemotherapeutic agents. Although the roles of a few miRNAs have been identified in cisplatin resistance, little is known in regards to the concerted contribution of miRNA‑mediated biological networks. In the present study, we demonstrated that microRNA-218 (miR-218) was significantly upregulated in cisplatin-resistant oral cancer cells. The results of cell viability and apoptosis assay showed that ectopic expressi...
Source: Oncology Reports - August 31, 2017 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research