Lysyl oxidase-like 2 is highly expressed in colorectal cancer cells and promotes the development of colorectal cancer.
Authors: Cui X, Wang G, Shen W, Huang Z, He H, Cui L Abstract Colorectal cancer (CRC) is the third most common type of cancer. In the present study, the expression and intracellular localization of lysyl oxidase-like 2 (LOXL2) protein in CRC were examined. Quantitative polymerase chain reaction and western blot analysis of LOXL2 mRNA and protein expression was performed for 40 pairs of CRC tumor and normal mucosa tissue samples. The immunohistochemical staining of tissue microarrays was performed to detect LOXL2 protein expression. LOXL2 was highly expressed in the extracellular matrix and CRC cells. The posit...
Source: Oncology Reports - June 1, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Microarray analysis of circular RNA expression profiles associated with gemcitabine resistance in pancreatic cancer cells.
Authors: Xu C, Yu Y, Ding F Abstract Pancreatic cancer (PC) is one of the most malignant tumors of the digestive system due to its rapid progression, metastasis and resistance to chemotherapy. Gemcitabine (GEM) chemotherapy is the first‑choice treatment for advanced PC. However, the effect of GEM‑based chemotherapy on PC is limited due to the development of chemoresistance, and the molecular mechanisms underlying this resistance have yet to be investigated. Circular RNAs (circRNAs), which can function as microRNA sponges, have been found to be involved in the development of several types of cancer. However, res...
Source: Oncology Reports - May 24, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Specific inhibitor of Notch ‑3 enhances the sensitivity of NSCLC cells to gemcitabine.
Specific inhibitor of Notch‑3 enhances the sensitivity of NSCLC cells to gemcitabine. Oncol Rep. 2018 May 16;: Authors: Hu BD, Guo J, Ye YZ, Du T, Cheng CS, Jiang Q, Liu RN, Zhang YB Abstract Notch‑3 is a receptor of the Notch signaling pathway and plays an important role in regulating self‑renewal, differentiation and apoptosis in cancer cells. Overexpression of Notch‑3 has been proved to be associated with resistance to gemcitabine (GEM) and poor patient prognosis for various malignant tumors. In the present study, two non‑small cell lung cancer (NSCLC) cell lines, H1299 and A549, were indu...
Source: Oncology Reports - May 24, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Knockdown of KPNA2 inhibits autophagy in oral squamous cell carcinoma cell lines by blocking p53 nuclear translocation.
Authors: Lin F, Gao L, Su Z, Cao X, Zhan Y, Li Y, Zhang B Abstract Oral squamous cell carcinoma (OSCC), one of the 10 most common types of neoplasms in the US, constitutes ~90% of all cases of oral malignancies. Chemoresistance and metastasis are difficult to avoid during the course of treatment, leading to a poor prognosis and a high mortality rate for patients with OSCC. Autophagy, a critical conserved cellular process, has been reported to be highly associated with the regulation of chemoresistance and metastasis of cancer cells. The present study investigated the role of karyopherin α2 (KPNA2), a mem...
Source: Oncology Reports - May 24, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Correlation between HGF/c-Met and Notch1 signaling pathways in human gastric cancer cells.
Authors: Huang KH, Sung IC, Fang WL, Chi CW, Yeh TS, Lee HC, Yin PH, Li AF, Wu CW, Shyr YM, Yang MH Abstract In recent decades, research concerning gastric carcinogenesis has rapidly progressed. It is evident that hepatocyte growth factor (HGF) is clinically related to gastric cancer progression and metastasis. In addition, previous studies have found that expression of Notch ligand Jagged1 is correlated with the poor prognosis of gastric cancer. However, the interaction between the HGF/c-Met and Notch1 signaling pathways remains unknown. In the present study, we found that gastric cancer patients with positive c-M...
Source: Oncology Reports - May 24, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA-485 plays tumour-suppressive roles in colorectal cancer by directly targeting GAB2.
Authors: Li J, Xu J, Yan X, Jin K, Li W, Zhang R Abstract Colorectal cancer (CRC) is reported to be the third most common cancer and the fourth leading cause of cancer-related deaths around the world. MicroRNA-485 (miR-485) has been reported to be aberrantly expressed and play important roles in several types of human malignancy. However, the expression level, biological functions and underlying molecular mechanisms of miR-485 in CRC remain unclear. Therefore, the aim of the present study was to determine miR-485 expression levels and their clinical significance in CRC and to explore the functions and underlying me...
Source: Oncology Reports - May 24, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Potential role of cyclin F mRNA expression in the survival of skin melanoma patients: Comprehensive analysis of the pathways altered due to cyclin F upregulation.
Authors: Gagat M, Krajewski A, Grzanka D, Grzanka A Abstract Cyclin F is a part of the Skp, Cullin, F-box containing ligase complex. The activity of cyclin F includes cell cycle control, centrosome duplication and response to DNA damage. The cyclin F expression pattern is very similar to cyclin A, but cyclin F is an orphan cyclin without its cyclin-dependent kinase partner. There is little evidence concerning the role of cyclin F in cancer. In the present study, for the first time, we present analysis from The Cancer Genome Atlas (TCGA) data in the context of expression of cyclin F mRNA in melan...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Fenretinide inhibits the proliferation and migration of human liver cancer HepG2 cells by downregulating the activation of myosin light chain kinase through the p38 ‑MAPK signaling pathway.
In conclusion, 4‑HPR inhibited the proliferation and migration of HepG2 cells, and p38‑MAPK plays an important role in regulating these 4‑HPR effects by reducing the activation of MLCK. The present study suggests that 4‑HPR may be a potent antimetastatic agent. PMID: 29767236 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

miR-142-5p regulates CD4+ T cells in human non-small cell lung cancer through PD-L1 expression via the PTEN pathway.
Authors: Wan J, Ling X, Peng B, Ding G Abstract The present study aimed to evaluate the function of microRNA (miR)-142-5p on cancer immunity to induce apoptosis in human non-small cell lung cancer (NSCLC) and its mechanism. miR-142-5p expression was upregulated, and CD4+ T cell levels were reduced in patients with NSCLC. Overexpression of miR-142-5p expression inhibited the cancer effects of CD4+ T cells on NSCLC cell lines, and downregulation of miR-142-5p increased the cancer effects of CD4+ T cells on NSCLC cell lines, compared with the control group. In addition, we found that overexpression of miR-142-5p suppr...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Comparison of the synergistic anticancer activity of AlPcS4 photodynamic therapy in combination with different low ‑dose chemotherapeutic agents on gastric cancer cells.
Comparison of the synergistic anticancer activity of AlPcS4 photodynamic therapy in combination with different low‑dose chemotherapeutic agents on gastric cancer cells. Oncol Rep. 2018 May 16;: Authors: Xin J, Wang S, Zhang L, Xin B, He Y, Wang J, Wang S, Shen L, Zhang Z, Yao C Abstract Limited cellular delivery and internalization efficiency of Al(III) phthalocyanine chloride tetrasulfonic acid (AlPcS4) induce poor penetration ability in cells and a slight photodynamic therapy (PDT) effect on gastric cancer. The combination treatment of AlPcS4/PDT with low‑dose chemotherapeutic agents may provide ...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Combined treatment with PI3K inhibitor BKM120 and PARP inhibitor olaparib is effective in inhibiting the gastric cancer cells with ARID1A deficiency.
Authors: Yang L, Yang G, Ding Y, Huang Y, Liu S, Zhou L, Wei W, Wang J, Hu G Abstract Dual blockade of phosphoinositide 3-kinase (PI3K) and poly(ADP-ribose) polymerase (PARP) has been revealed to be an effective treatment strategy for breast, ovarian and prostate cancer. However, the efficacy of this combination for the treatment of gastric cancer, and potential predictive therapeutic biomarkers remain unclear. Recent evidence suggests that the deficiency of AT-rich interactive domain containing protein 1A (ARID1A), which is a crucial chromatin remodeling gene, sensitizes tumor cells to PI3K and PARP...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Umbilical cord ‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells.
Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells. Oncol Rep. 2018 May 16;: Authors: Wang W, Li L, Chen F, Yang Y Abstract Tumor tropism is an important property of mesenchymal stem cells (MSCs) that has been used in tumor‑targeting therapies. However, the effects of MSCs on tumors remain controversial. The aim of the present study was to investigate the effects of MSCs on A375 melanoma cells. Umbilical cord‑derived mesenchymal stem cells (UCMSCs) were co‑cultured with A375 cells. MTT and Transwell assays were used to assess cell prolifera...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

PD-L1 mRNA expression in EGFR-mutant lung adenocarcinoma.
Authors: Isobe K, Kakimoto A, Mikami T, Kaburaki K, Kobayashi H, Yoshizawa T, Nakano Y, Makino T, Otsuka H, Sano G, Sugino K, Sakamoto S, Takai Y, Tochigi N, Iyoda A, Homma S Abstract Molecular mechanisms of programmed death-ligand 1 (PD-L1) mRNA expression and roles of apoptosis and biomarkers are poorly understood in epidermal growth factor receptor (EGFR)-mutant lung adenocarcinoma patients. Thirty-three patients with recurrent postoperative EGFR-mutant lung adenocarcinoma (exon 19 deletion in 16, L858R in 15, G719C in 2 patients) treated with gefitinib were studied. PD-L1 mRNA expression of formalin-fixed ...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma.
Authors: Iwata E, Hasegawa T, Ueha T, Takeda D, Saito I, Kawamoto T, Akisue T, Sakai Y, Sasaki R, Kuroda R, Komori T Abstract Radiotherapy (RT) is one of the main treatment modalities for oral squamous cell carcinoma (OSCC), however, radioresistance is a major impediment to its clinical success and poses as a concern that needs to be addressed. Tumor hypoxia is known to be significantly associated with radioresistance in various malignancies, hence, resolving the hypoxic state of a tumor may improve the antitumor effect of RT on OSCC. We have previously revealed that transcutaneous CO2 induced mitochondrial apoptos...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Elemental diet inhibits pro ‑inflammatory cytokine production in keratinocytes through the suppression of NF‑κB activation.
Elemental diet inhibits pro‑inflammatory cytokine production in keratinocytes through the suppression of NF‑κB activation. Oncol Rep. 2018 May 16;: Authors: Harada K, Ferdous T, Mizukami Y, Mishima K Abstract An elemental diet (ED) has been reported to reduce oral mucositis and dermatitis induced by chemotherapy. However, its molecular mechanism of action as an anti‑inflammatory agent is still unknown. The aim of the present study was to clarify whether ED confers its anti‑inflammatory action via reduction of pro‑inflammatory cytokine production in keratinocytes in vivo and in ...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibition of SOX4 induces melanoma cell apoptosis via downregulation of NF- κB p65 signaling.
In conclusion, our results demonstrated that inhibition of SOX4 markedly induced melanoma cell apoptosis via downregulation of the NF-κB signaling pathway, which thus may be a novel approach for the treatment of melanoma. PMID: 29767266 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

HDAC inhibitors, trichostatin A and valproic acid, increase E ‑cadherin and vimentin expression but inhibit migration and invasion of cholangiocarcinoma cells.
In conclusion, co‑treatment of cholangiocarcinoma cells with TSA or VPA and GEM suppressed EMT with tolerable cytotoxicity. However, the HDAC inhibitors augmented both E‑cadherin and vimentin expression and their effects varied in different cholangiocarcinoma cell lines. Therefore, the clinical use of HDAC inhibitors in biliary cancer should be considered cautiously. PMID: 29767267 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

CHAF1B knockdown blocks migration in a hepatocellular carcinoma model.
Authors: Peng X, Fu H, Yin J, Zhao Q Abstract The roles and model of action of the chromatin assembly complex factor-1B (CHAF1B) gene in liver cancer have not been fully elucidated. The CHAF1B gene in human hepatocellular carcinoma cell line HUH-7 was knocked down using a lentivirus and the transfected cells were assayed for migration and invasion abilities and cell cycle arrest using the scratch wound healingand Transwell assays as well as flow cytometry, respectively. Cells transfected with an empty vector were used as the control. The expression of genes was profiled. Models were constructed using CHAF1B-knockdo...
Source: Oncology Reports - May 18, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

βKlotho inhibits androgen/androgen receptor‑associated epithelial‑mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling.
In conclusion, βKlotho inhibits EMT and plays a tumor‑suppressive role in PCa, linking FGF/FGFR/βKlotho signaling to the regulation of PCa progression. PMID: 29749458 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

KIF20A expression as a prognostic indicator and its possible involvement in the proliferation of ovarian clear ‑cell carcinoma cells.
We examined the distribution and expression of KIF20A in clear‑cell carcinoma (CCC) of the ovary to elucidate its clinical significance and molecular mechanism. Paraffin sections from ovarian CCC tissues (N=43) were immunostained with KIF20A antibody, and the staining intensities were semi‑quantitatively evaluated. Furthermore, we investigated whether silencing of KIF20A contributes to the proliferation‑inhibitory potential using CCC cells. During the observational period, 18 patients (41.9%) developed recurrence. The median time to recurrence was 11.5 months. Patients in the high KIF20A expression group showed ...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Insufficient radiofrequency ablation promotes hepatocellular carcinoma cell progression via autophagy and the CD133 feedback loop.
Authors: Wang X, Deng Q, Feng K, Chen S, Jiang J, Xia F, Ma K, Bie P Abstract Insufficient radiofrequency ablation (iRFA) often leads to residual hepatocellular carcinoma (HCC) progression. However, the mechanism is still poorly understood. In the present study, we demonstrated that LC3B protein expression levels were significantly increased in the residual hepatocellular carcinoma cells after radiofrequency ablation (RFA) treatment in vivo. Moreover, iRFA promoted autophagy, autophagosome formation and autophagic flux in Huh-7 and SMMC7721 cell lines in vitro. In addition, iRFA induced HCC cell viability...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Effect of a novel orally bioavailable CXCR4 inhibitor, AMD070, on the metastasis of oral cancer cells.
Authors: Uchida D, Kuribayashi N, Kinouchi M, Sawatani Y, Shimura M, Mori T, Hasegawa T, Miyamoto Y, Kawamata H Abstract We have previously demonstrated that the stromal cell‑derived factor (SDF‑1)/CXCR4 system is involved in the metastasis of head and neck cancer. Additionally, it has been revealed that the blockade of CXCR4 by subcutaneous daily injection with AMD3100, a CXCR4 antagonist, may be effective in preventing metastasis in CXCR4‑related head and neck cancer. Recent investigations have suggested that AMD070, a novel orally bioavailable inhibitor of CXCR4, may be minimally invasive compared wit...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Protein arginine methyltransferase 5 is implicated in the aggressiveness of human hepatocellular carcinoma and controls the invasive activity of cancer cells.
Authors: Jeon JY, Lee JS, Park ER, Shen YN, Kim MY, Shin HJ, Joo HY, Cho EH, Moon SM, Shin US, Park SH, Han CJ, Choi DW, Gu MB, Kim SB, Lee KH Abstract Protein arginine methyltransferase 5 (PRMT5) is a protein that catalyzes transfer of methyl groups to the arginine residues of proteins and is involved in diverse cellular and biological responses. While the participation of PRMT5 in cancer progression has been increasingly documented, its association with the invasive phenotype currently remains poorly understood. In the present study, we revealed that PRMT5 is overexpressed in human hepatocellular carcinoma&n...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Morusin suppresses A549 cell migration and induces cell apoptosis by downregulating the expression of COX ‑2 and VEGF genes.
In conclusion, morusin significantly inhibited the proliferation of the lung cancer cell line A549, and may have affected the invasion and migration of the cells by downregulating the expression of tumor angiogenesis‑related genes. PMID: 29749480 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Integrated bioinformatics analysis reveals that the expression of cathepsin  S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer.
Integrated bioinformatics analysis reveals that the expression of cathepsin S is associated with lymph node metastasis and poor prognosis in papillary thyroid cancer. Oncol Rep. 2018 May 08;: Authors: Tan J, Qian X, Song B, An X, Cai T, Zuo Z, Ding D, Lu Y, Li H Abstract The prognosis of the majority of patients with papillary thyroid cancer (PTC) is excellent, although there are patients who experience disease recurrence and progression. The aim of the present study was to identify potential prognostic risk markers in PTC. Differentially expressed genes (DEGs), identified from four Genome Ex...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Use of CA15 ‑3 for screening breast cancer: An antibody‑lectin sandwich assay for detecting glycosylation of CA15‑3 in sera.
Use of CA15‑3 for screening breast cancer: An antibody‑lectin sandwich assay for detecting glycosylation of CA15‑3 in sera. Oncol Rep. 2018 May 10;: Authors: Choi JW, Moon BI, Lee JW, Kim HJ, Jin Y, Kim HJ Abstract Elevated serum CA15‑3 assessed by enzyme‑linked immunosorbent assay (ELISA) has been considered a diagnostic marker of breast cancer. However, accumulating data indicate that the current ELISA system for detecting CA15‑3, which targets the peptide backbone of CA15‑3, is not sufficiently sensitive to detect early or localized breast cancer. In the present study, we designed an a...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Downregulation of RPN2 induces apoptosis and inhibits migration and invasion in colon carcinoma.
Authors: Bi C, Jiang B Abstract The morbidity of colorectal cancer (CRC) increases annualy, which accounts to higher mortality worldwide. Therefore, it is important to study the pathogenesis of colon cancer. Ribophorin II (RPN2), part of the N-oligosaccharyltransferase complex, is highly expressed in CRC. In the present study, we investigated whether RPN2 can regulate apoptosis, migration and invasion by RNA interference in CRC and sought to clarify the molecular mechanism involved. Based on previous research, an abnormal high expression of RPN2 was observed in CRC tissues and cell lines by real-time (RT)...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Stable silencing of dll4 gene suppresses the growth and metastasis of esophagus cancer cells by attenuating Akt phosphorylation.
Authors: Guo X, Duan Y, Ye X, Hu L, Xu T, Tong L, Yu M Abstract δ‑Like ligand 4 (DLL4) has recently been reported to be involved in the process of cancer angiogenesis and considered to play a vital role in vascular endothelial growth factor (VEGF) signaling, while the role of DLL4 in cancer metastasis and growth has not been systematically studied. In the present study, the esophagus cancer cell line Eca109 was infected in vitro with a lentiviral vector loaded with dll4‑shRNA to obtain a stable cell line of DLL4 expression which was downregulated through puromycin screening. The migration and in...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

LSD1 negatively regulates autophagy through the mTOR signaling pathway in ovarian cancer cells.
Authors: Wei Y, Han T, Wang R, Wei J, Peng K, Lin Q, Shao G Abstract Lysine-specific demethylase 1 (LSD1) plays a key role in cell proliferation, differentiation and carcinogenesis. In the present study we revealed that LSD1 functioned as an autophagy suppressor in ovarian cancer HO8910 cells. Pharmacological inhibition or genetic knockdown of LSD1 resulted in the elevation of the LC3‑II protein, enhancement of autophagosomal formation and stimulation of the autophagic flux. In addition, knockdown of LSD1 further promoted the serum starvation- and rapamycin-induced autophagy. Furthermore, we demonstrated tha...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Inhibition of endoplasmic reticulum stress-induced autophagy sensitizes melanoma cells to temozolomide treatment.
Authors: Ryabaya O, Prokofieva A, Khochenkov D, Abramov I, Zasedatelev A, Stepanova E Abstract The incidence of malignant melanoma is increasing. The discovery of agents specifically targeting the mutated cascades has provided a good response for patients with oncogenic B-Raf proto-оncogene, serine/threonine kinase (BRAF). However, numerous studies continue to focus on novel methods of treatment to overcome acquired resistance to novel drugs. Recently, it has been revealed that inhibition of endoplasmic reticulum (ER) stress chaperon 78 kDa glucose-regulated protein 78 (GRP78) leads to down-regulation of auto...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Multifaceted regulation and functions of YAP/TAZ in tumors (Review).
Authors: Liu H, Du S, Lei T, Wang H, He X, Tong R, Wang Y Abstract The Hippo pathway, initially identified through screenings for mutant tumor suppressors in Drosophila, is an evolutionarily conserved signaling pathway that controls organ size by regulating cell proliferation and apoptosis. Abnormal regulation of the Hippo pathway may lead to cancer in mammals. As the major downstream effectors of the Hippo pathway, unphosphorylated Yes-associated protein (YAP) and its homolog transcriptional co-activator TAZ (also called WWTR1) (hereafter called YAP/TAZ) are translocated into the nucleus. In the nucleus, in order ...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Purple sweet potato anthocyanin exerts antitumor effect in bladder cancer.
Authors: Li WL, Yu HY, Zhang XJ, Ke M, Hong T Abstract Bladder cancer (BC) is the most common malignant disease. The developing of economically sustainable and available agents for the treatment of BC is required. Purple sweet potato anthocyanin (PSPA) has been shown to have antitumor abilities. The present study aimed to evaluate the potential role of PSPA in BC treatment. CCK-8 assay was used to assess the viability of BC cells. Flow cytometry assays were performed to evaluate the mitochondrial membrane potential (MMP), cell apoptosis and cell-cycle distribution. Real-time PCR (RT-PCR) and western blot analysis w...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Methylation of the claudin ‑3 promoter predicts the prognosis of advanced gastric adenocarcinoma.
Methylation of the claudin‑3 promoter predicts the prognosis of advanced gastric adenocarcinoma. Oncol Rep. 2018 May 02;: Authors: Zhang Z, Yu W, Chen S, Chen Y, Chen L, Zhang S Abstract Claudin‑3 expression is associated with gastric cancer progression, but the role of epigenetic modifications remains unclear. We investigated methylation of the claudin‑3 promoter and expression profiles in gastric adenocarcinoma and their associations with clinicopathological characteristics and prognosis of the patients. A total of 122 patients with advanced gastric cancer [stage IIB‑IV, with lymph node ...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Long non-coding RNA SNHG1 predicts a poor prognosis and promotes colon cancer tumorigenesis.
Authors: Yang H, Wang S, Kang YJ, Wang C, Xu Y, Zhang Y, Jiang Z Abstract Colon cancer is the main cause of cancer mortality worldwide. Its poor prognosis is mainly ascribed to high recurrence rates. Identifying novel prognostic biomarkers and therapeutic key points for management is crucial and important. Long non-coding RNAs (lncRNAs) are a class of RNAs, which have various roles in carcinogenicity and molecular mechanisms. The lncRNA small nucleolar RNA host gene 1 (SNHG1) contributes to the promotion of tumor development, however, the connections between SNHG1 and colon cancer are still unclear. The aim of...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF.
In conclusion, miR-125b had an antitumor role in ESCC cells mediated by targeting BMF, which can be potentially useful for tumorigenesis in ESCC. PMID: 29749531 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

MEG3/miR ‑21 axis affects cell mobility by suppressing epithelial‑mesenchymal transition in gastric cancer.
In conclusion, we demonstrated that the MEG3/miR‑21 axis participates in the tumor progression and metastasis of gastric cancer through the regulation of EMT. PMID: 29749532 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non ‑small cell lung cancer cells.
EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells. Oncol Rep. 2018 May 08;: Authors: Lin C, Qin Y, Zhang H, Gao MY, Wang YF Abstract Activation of the epidermal growth factor receptor (EGFR) during tumor development can trigger the MEK signaling pathway. In the present study, we investigated the MEK signaling pathway in non‑small cell lung cancer (NSCLC) cells with respect to the effect of epidermal growth factor (EGF) on expression of Ret finger protein like 3 (RFPL3) and human telomerase reverse transcriptase (hTERT), and the effect of RFPL3 ov...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Oncogenic role of miR ‑183‑5p in lung adenocarcinoma: A comprehensive study of qPCR, in vitro experiments and bioinformatic analysis.
Oncogenic role of miR‑183‑5p in lung adenocarcinoma: A comprehensive study of qPCR, in vitro experiments and bioinformatic analysis. Oncol Rep. 2018 May 09;: Authors: He RQ, Gao L, Ma J, Li ZY, Hu XH, Chen G Abstract Despite the fact that previous studies have reported the aberrant expression of miR‑183‑5p in lung adenocarcinoma (LUAD), the oncogenic role of miR‑183‑5p in LUAD and its underlying mechanisms have remained elusive. Hence, we attempted to elucidate the clinicopathological significance of miR‑183‑5p expression in LUAD and identify the biological function of miR‑1...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

BIIB021, an Hsp90 inhibitor: A promising therapeutic strategy for blood malignancies (Review).
Authors: He W, Hu H Abstract Heat shock proteins (HSPs) are molecular chaperones that are consistently increased to help cells survive under conditions of stress. As a member of the Hsps, Hsp90 is involved in protein post‑translational maturation and disposition. This protein is ubiquitously expressed in normal cells. However, in cancer cells and particularly in hematological malignancies, Hsp90 is unexpectedly abundant to maintain levels of proteins vital for cancer pathology. Hsp90 inhibitors can target the ATP domain of Hsp90 and prohibit its exchange of ADP for ATP, leading to the degradation of client protei...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Exosome ‑encapsulated microRNA‑23b as a minimally invasive liquid biomarker for the prediction of recurrence and prognosis of gastric cancer patients in each tumor stage.
Exosome‑encapsulated microRNA‑23b as a minimally invasive liquid biomarker for the prediction of recurrence and prognosis of gastric cancer patients in each tumor stage. Oncol Rep. 2018 May 08;: Authors: Kumata Y, Iinuma H, Suzuki Y, Tsukahara D, Midorikawa H, Igarashi Y, Soeda N, Kiyokawa T, Horikawa M, Fukushima R Abstract Recently, exosome‑encapsulated microRNAs (miRNAs) have been attracting attention as stable and minimally invasive biomarkers in cancer patients. The aim of the present study was to clarify the value of plasma exosomal microRNA‑23b (miR‑23b) as a diagnostic and prognostic ...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

TRIM58/cg26157385 methylation is associated with eight prognostic genes in lung squamous cell carcinoma.
Authors: Zhang W, Cui Q, Qu W, Ding X, Jiang D, Liu H Abstract The present study aimed to analyze the differentially expressed genes related to the tripartite motif containing 58 (TRIM58)/cg26157385 methylation sites, and consequently to provide theoretical basis for elucidating the influence of TRIM58/cg26157385 methylation on lung cancer prognosis. Methylation‑sequencing information, mRNA expression profiling data and clinical data were downloaded from cBioPortal database to screen out candidate genes related to the methylation of TRIM58/cg26157385 in squamous cell lung carcinoma. The differentially expressed g...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Somatic copy number alterations have prognostic impact in patients with ovarian clear cell carcinoma.
Authors: Morikawa A, Hayashi T, Kobayashi M, Kato Y, Shirahige K, Itoh T, Urashima M, Okamoto A, Akiyama T Abstract Ovarian clear cell carcinoma (OCCC) is a chemotherapy‑resistant epithelial ovarian cancer with poor prognosis. To identify genomic alterations involved in the development of OCCC, we analyzed somatic copy number alterations in OCCC using comparative genomic hybridization (CGH). Here we showed that the chromosomal regions 8p11.21, 8p11.22, 12p13.31 and 20q13.2 were amplified in OCCC. We also demonstrated that small segments in the chromosomal regions 3q26.1, 4q13.2 and 22q11.23 were deleted...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Suppression of RRM2 inhibits cell proliferation, causes cell cycle arrest and promotes the apoptosis of human neuroblastoma cells and in human neuroblastoma RRM2 is suppressed following chemotherapy.
Authors: Li J, Pang J, Liu Y, Zhang J, Zhang C, Shen G, Song L Abstract Ribonucleotide reductase regulatory subunit M2 (RRM2) is a rate‑limiting enzyme for DNA synthesis and repair. RRM2 has vital roles in controlling the progression of cancer. In the present study, we investigated the RRM2 level in neuroblastoma tissues, analyzed its relationship with clinicopathological characteristics of neuroblastoma patients, and explored the effect of RRM2 on the biological functions of neuroblastoma cells. RRM2 levels in 67 pairs of neuroblastoma and matched adjacent non‑cancerous tissues were detected by qRT‑PCR,...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Tumour cell dormancy as a contributor to the reduced survival of GBM patients who received standard therapy.
Authors: Tong L, Yi L, Liu P, Abeysekera IR, Hai L, Li T, Tao Z, Ma H, Xie Y, Huang Y, Yu S, Li J, Yuan F, Yang X Abstract Glioblastoma multiforme (GBM) is a fatal cancer with varying life expectancy, even for patients undergoing the same standard therapy. Identification of differentially expressed genes in GBM patients with different survival rates may benefit the development of effective therapeutic strategies. In the present study, key pathways and genes correlated with survival in GBM patients were screened with bioinformatic analysis. Included in the study were 136 eligible patients who had undertaken sur...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Microarray ‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC.
Microarray‑based bioinformatics analysis of the prospective target gene network of key miRNAs influenced by long non‑coding RNA PVT1 in HCC. Oncol Rep. 2018 May 02;: Authors: Zhang Y, Mo WJ, Wang X, Zhang TT, Qin Y, Wang HL, Chen G, Wei DM, Dang YW Abstract The long non‑coding RNA (lncRNA) PVT1 plays vital roles in the tumorigenesis and development of various types of cancer. However, the potential expression profiling, functions and pathways of PVT1 in HCC remain unknown. PVT1 was knocked down in SMMC‑7721 cells, and a miRNA microarray analysis was performed to detect the differentially expres...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

EBV ‑LMP1 is involved in vasculogenic mimicry formation via VEGFA/VEGFR1 signaling in nasopharyngeal carcinoma.
EBV‑LMP1 is involved in vasculogenic mimicry formation via VEGFA/VEGFR1 signaling in nasopharyngeal carcinoma. Oncol Rep. 2018 May 03;: Authors: Xu S, Bai J, Zhuan Z, Li B, Zhang Z, Wu X, Luo X, Yang L Abstract The Epstein-Barr virus latent membrane protein 1 (EBV‑LMP1) is an oncoviral protein that plays an important role in oncogenic transformation in EBV‑associated nasopharyngeal carcinoma (NPC). Our previous studies demonstrated that LMP1 increased VEGFA expression and upregulated angiogenesis in NPC. Vasculogenic mimicry (VM) is a mechanism by which tumor cells can obtain nutrients ...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Enhanced production of reactive oxygen species in HeLa cells under concurrent low ‑dose carboplatin and Photofrin® photodynamic therapy.
In conclusion, the therapeutic enhancement of ccPDT in vitro was attributable to the carboplatin‑mediated synergetic production of OH▪ and apoptotic cellular damage, compared with Photofrin‑PDT alone. PMID: 29749554 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

ClC-3 chloride channel protein induces G1  arrest in hepatocellular carcinoma Hep3B cells.
In conclusion, we demonstrated that ClC-3 can arrest the cell cycle at the G1 phase to inhibit cell proliferation, suggesting that ClC-3 has the potential to be a novel target for HCC therapy and potentially improve the prognosis of HCC patients. PMID: 29749557 [PubMed - as supplied by publisher] (Source: Oncology Reports)
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Endogenous Leu332Gln mutation in p53 disrupts the tetramerization ability in a canine mammary gland tumor cell line.
Authors: Ochiai K, Azakami D, Morimatsu M, Hirama H, Kawakami S, Nakagawa T, Michishita M, Egusa AS, Sasaki T, Watanabe M, Omi T Abstract Mutations in the p53 gene are associated with more than half of all human cancers. These mutations often cause a disruption of the tumor-suppressor function of p53 and induce genomic instabilities. Wild‑type p53 requires tetramerization to function as an initiator of cell cycle arrest and apoptosis. Although alterations in p53 tetramerization caused by mutation have been well studied, there are few cell lines containing an endogenous mutation in the tetramerization domain of p5...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Downregulation of occludin affects the proliferation, apoptosis and metastatic properties of human lung carcinoma.
Authors: Wang M, Liu Y, Qian X, Wei N, Tang Y, Yang J Abstract Lung cancer is the most frequent and deadliest cancer in the world, especially in China. However, the molecular mechanisms involved in lung cancer remain unclear. Occludin (OCLN), one of the first identified tight junction proteins, has been revealed to be a necessary integral protein for tight junction structure and function. In the present study, we investigated the role of occludin in lung tumorigenesis. We found that occludin protein expression was highly increased in human lung cancer patient samples. Western blotting results also revealed that occ...
Source: Oncology Reports - May 12, 2018 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research