Repeated 28-day oral dose toxicity of onion skin quercetin in mice
AbstractThe study was aimed at evaluating the 28-day repeated dose oral toxicity of onion skin quercetin (OSQ) in mice. Twenty-five mice were divided into five groups of 5 mice each. Group 1 served as the control, and groups 2 and 3 received OSQ at 95  mg/kg and 190 mg/kg, respectively, while groups 4 and 5 received OSQ at 380 mg/kg for 28 consecutive days. Mice in groups 1–4 were euthanized on day 29, while the mice in group 5 were left for 14 days without treatment before euthanasia. The kidneys and liver were processed for light microsco py, while the levels of AST, ALT, albumin, total protein ...
Source: Comparative Clinical Pathology - October 23, 2020 Category: Pathology Source Type: research

Effects of methanolic leaf extract of Pterocarpus santalinoides on the recovery from gentamicin-induced nephrotoxicity in albino rats
This study evaluated the effects of methanolic leaf extract ofPterocarpus santalinoides (MLEPS) on the recovery from gentamicin-induced nephrotoxicity in albino rats. Fresh leaves ofP. santalinoides were collected, dried, pulverized, and extracted by cold maceration technique using 80% methanol. Thirty male albino rats randomly assigned into six groups (A –F) of five rats each were used for the study. Nephrotoxicity was induced in groups A–E by intra-peritoneal administration of gentamicin (GM) once a day at 100 mg/kg for 10 days. Thereafter, the nephropathic (NP) rat groups (A–E) were used to a...
Source: Comparative Clinical Pathology - October 22, 2020 Category: Pathology Source Type: research

Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms
AbstractThe aim of this study was to investigate glycoconjugate immunostaining of the biotinylated lectins: UEA -Ulex europaeus (gorse), PNA -Arachis hypogaea (peanut), HPA -Helix pomatia (Roman snail), and DBA -Dolichos biflorus, in normal mammary glands (control;n = 7), mammary neoplasms (n = 111), and mammary neoplasm metastasis (n = 10). One hundred twenty-eight mammary gland tissue samples were used; 7 samples without history of neoplasms (control). The remaining samples (121) were mammary neoplasms or metastasis. In all cases (UEA, HPA, PNA, and DBA), the binding to normal epithelium was uniform. The immunohistochemi...
Source: Comparative Clinical Pathology - October 21, 2020 Category: Pathology Source Type: research

The effects of treatments with multistrain probiotics on serum aminotransferases, AST-ALT ratio, body weight and survivability scores of rats experimentally infected with Trypanosoma brucei
AbstractProbiotics can modulate gut-liver axis. Effects of administration of multistrain probiotics (MP) on liver damage and overall health of trypanosomic rats were investigated. Thirty male rats randomly assigned to five groups A –E (n = 6), and MP containingBifidobacterium BB-12,Lactobacillus delbrueckii LBY-27,Lactobacillus acidophilus LA-Z,Streptococcus thermophilus STY-31 andLactobacillus paracasei LC-01 were used for supplementation. From day 0 post-supplementation (PS), rats in groups A –C received 1 × 109, 5  × 109 and 10  × 109 ...
Source: Comparative Clinical Pathology - October 20, 2020 Category: Pathology Source Type: research

The regulatory role of exosomal CagA and microRNAs derived from H. pylori -related gastric cancer cells on signaling pathways related to cancer development: a bioinformatics aspect
AbstractExosomes are small vesicles outside the membrane, which are secreted by healthy, cancerous, and bacterial infected cells. These worthy vesicles carry shared information between adjacent cells and cause cell-cell communication. Bacteria can also secrete vesicles similar to exosomes called outer membrane vesicles (OMVs). The OMVs, also called quasi-exosomes due to its similarity to exosomes, are bacterial extracellular membrane vesicles that are secreted to enhance the pathogenicity of the bacteria. The continued secretion of exosome by cells and quasi-exosome by bacteria in gastric cancer played a vital role in canc...
Source: Comparative Clinical Pathology - October 20, 2020 Category: Pathology Source Type: research

Extracts of Hura crepitans L. stem bark attenuate liver injury and inflammation induced by CCl 4 in rats
This study evaluated the hepatoprotective effects of the aqueous (AE) and ethanolic extracts (EE) ofH. crepitans stem bark in CCl4-induced liver injury in Sprague-Dawley rats. Liver injury was induced by single oral administration of 1 mL/kg b.wt CCl4 (1:1 v/v olive oil). The effect of AE and EE was assessed at 250 mg/kg p.o. Serum and liver biochemical markers were measured, and liver was assessed for oxidative stress, morphological changes, and expression of some pro-inflammatory cytokines. EE, AE, and silymarin displayed significant hepatoprotective activities by ameliorating the activities of transaminase (ALT and AST)...
Source: Comparative Clinical Pathology - October 13, 2020 Category: Pathology Source Type: research

Therapeutic evaluation of anti-trypanosoma activity of ethanol extracts of Jatropha curcas roots in comparison with diminazene aceturate in Trypanosoma brucei brucei –parasitized rats
This study showed that EEJCR is safe, has anti-trypanosoma activity and reverses haematological and biochemi cal markers of organ malfunctions associated with trypanosoma infection. This observation justifies the old routine use of such herbal therapy in medicinal practices to treat trypanosomiasis. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 30, 2020 Category: Pathology Source Type: research

Assessment of cholinesterase activity and hepatic biofunction in dogs naturally infected with Babesia gibsoni
This study investigated the activity of cholinergic system enzymes and hepatic biofunction in dogs naturally infected withBabesia gibsoni. An infected group comprised 10 male dogs (stray and shepherd), and 10 healthy dogs as control were included in the study. Hematological parameters and activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were measured. The biomarkers of hepatic function including the activities of various enzymes, total bilirubin levels, and albumin contents were also assessed. The infected animals revealed a significant decrease in RBC count, packed cell volume, hemoglobin concent...
Source: Comparative Clinical Pathology - September 14, 2020 Category: Pathology Source Type: research

In vitro antioxidants and haematological and nephroprotective effects of biscuits produced from three medicinal flour blends fed to high-fat diet/streptozotocin (STZ)-induced diabetic rats
This study investigated the in vitro antioxidants and haematological and nephroprotective effects of biscuits produced from three medicinal flour blends fed to high-fat diet/streptozotocin (STZ)-induced diabetic rats. The biscuits were produced at different proportions of flour blends. The total phenol and flavonoid, FRAP, DPPH, and α-amylase and glucosidase of the biscuits were determined. The White Albino Wistar rats were induced with streptozotocin (STZ) and fed with the formulated biscuits for 14 days. The animals were sacrificed, and blood was obtained and used to determine haematological parameter. Then, t...
Source: Comparative Clinical Pathology - September 9, 2020 Category: Pathology Source Type: research

Experimental infection by Aeromonas hydrophila in Piaractus mesopotamicus : DL50, neurological disturbances, and mortality
This study aimed to evaluate the physiopathology manifestations of septic aeromonosis inPiaractus mesopotamicus, considering its clinical manifestations, mortality rates, anatomopathology, and anti-iNOS immunolabeling in the brain. Fish were inoculated in the coelomic cavity with increasing concentrations ofA. hydrophila: 1.5  × 108; 1.8  × 108; 2.1  × 108; 2.4  × 108; 2.7  × 108 CF mL−1, histopathology, and anti-iNOS immunolabeling in brain. The animals present erratic swimming (swirling), depigmentation, petechiae, s...
Source: Comparative Clinical Pathology - September 7, 2020 Category: Pathology Source Type: research

D-ribose-L-cysteine improves hormonal imbalance, spermatogenic dysregulation, and redox status in streptozotocin-diabetic rats
This study is to investigate the potency of D-ribose-L-cysteine in reversing hyperglycemia and reproductive histopathological alterations in streptozotocin-diabetic rats. Diabetes was induced in adult male Wistar rats with a single intraperitoneal injection of 70  mg/kg b.w. streptozotocin. The hyperglycemic rats were thereafter treated orally with D-ribose-L-cysteine (100 mg/kg b.w.) and metformin (30 mg/kg b.w.) respectively once daily for a period of 17 days. Blood glucose concentration, reproductive hormonal assays, seminal fluid analysis, testicular anti-oxidant enzymes, tissue protein, and histopa...
Source: Comparative Clinical Pathology - September 3, 2020 Category: Pathology Source Type: research

Preventive effect of Stachys lavandulifolia against gentamicin-induced oxidative stress and nephrotoxicity in rats
In this study, forty-two male Wistar rats were randomly divided into six equal groups (N = 7 each): control (group I), GM-treated (group II), SL200 mg/kg + GM-treated (group III), SL400 mg/kg + GM-treated (group IV), SL200 mg/kg-treated (group V), and SL400 mg/kg-treated (group VI) groups. The treatment procedures continued for 10 days. At the end of the experiment, seru m and the kidneys of all groups were harvested for histological and biochemical examination. No statistical significant improvement in histological and biochemical indices was shown at 200 mg/kg...
Source: Comparative Clinical Pathology - September 1, 2020 Category: Pathology Source Type: research

Effect of sex on hematology, morphology and blood cell characteristics of Schizothorax niger
AbstractStudy of hematological parameters, blood cell morphology and its characteristics are considered as essential tools to evaluate the health status of fish species. Among snow trout species,Schizothorax niger contribute a significant proportion to the overall fish production in the region, thus considered as an important food fish species. However, the population of this fish species is declining day by day from the natural habitats due to one or another reason. For understanding the health status of the fish an attempt has been made to establish sex wise base line data on hematological parameters, blood cell morpholo...
Source: Comparative Clinical Pathology - August 28, 2020 Category: Pathology Source Type: research

Correction to: Existence of immune cells in uterine luminal flush of repeat breeding Egyptian buffalo-cow ( Bubalus bubalis ): salpingitis and endometritis as a major cause
This paper was published without table and figures. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - August 25, 2020 Category: Pathology Source Type: research

Validation of the FAMACHA © system for detecting anaemia and helminthosis in West African Dwarf sheep in Makurdi, Benue State, Nigeria
AbstractGastrointestinal helminthes are a major cause of unproductiveness in the sub-Saharan Africa livestock industry. The use of anthelmintic has been the most reliable means of preventing losses due to gastrointestinal parasitism. Due to the challenge of anthelmintic resistance, however, the concept of targeted selective treatment (TST) using the FAMACHA© system was developed. The system has been validated in several countries and in West African Dwarf goats in Nigeria. There is, however, limited information on the use of the FAMACHA© system in West African Dwarf (WAD) sheep in Nigeria. A total of twenty WAD s...
Source: Comparative Clinical Pathology - August 21, 2020 Category: Pathology Source Type: research

Protective effects of apigenin against Bisphenol A-induced testis toxicity in Wistar rats through modulating hepatic biochemical biomarkers and histological changes
This study has been designed to investigate the ameliorating effect of APG against oxidative stress induced by bisphenol A(BPA) in the liver and testis of male rats. Rats were divided into five groups of five animals each: G1 (control group), G2 (BPA 1  mg/kg B.wt), G3 (BPA 10 mg/kg B.wt), G4 (BPA + APG; 1 + 20 mg/kg/B.wt) and G5 (BPA + APG; 10 + 20 mg / kg/B.wt).The treatments have been given by gavage to rats daily for 30 days. After the last day of treatment, rats were sacrificed by decapitation after exposure to ether. The results showed t...
Source: Comparative Clinical Pathology - August 21, 2020 Category: Pathology Source Type: research

Prognostic measurement for equines with colic
AbstractColic syndrome is considered one of the main diseases in equine medicine due to the mortality involved. This alteration of the gastrointestinal tract, mainly ischemic, may cause changes in glucose and lactate values in blood and peritoneal fluid samples. Thus, the aim of this study was to evaluate the concentrations of glucose and lactate in peripheral blood and peritoneal fluid in horses with different etiologies and degrees of severity of colic. The time to presentation, type of colic, evolution, and treatment were also evaluated by clinical examination and surgical procedure. Hyperglycemia was observed in 66.6% ...
Source: Comparative Clinical Pathology - August 17, 2020 Category: Pathology Source Type: research

The effects of sex and aging on selected plasma biochemistry analytes in zoo-kept American flamingos ( Phoenicopterus ruber )
AbstractAmerican flamingos (Phoenicopterus ruber) are commonly kept in zoological collections and are important in research, conservation awareness, and education. Hematology and biochemistry parameters are reported to change with age in many long-lived species. A retrospective analysis of recorded data (147 samples of PCV/TS and plasma biochemistry) fromn = 16 (F = 7, M = 9) zoo-kept flamingos (1–16 years) was performed. Blood samples were collected as part of routine annual health evaluation over the course of 11 years. The effect of aging and sex on biochemical a...
Source: Comparative Clinical Pathology - August 16, 2020 Category: Pathology Source Type: research

Alkaloid and terpenoid fractions from the leaves of Launaea taraxacifolia (Willd.) repressed the proliferation of Plasmodium berghei in mice
AbstractMedicinal plants play a major role in the treatment and prevention of malaria in many communities across the world. The aim of the study was to validate the ethno pharmacological claims ofL. taraxacifolia, traditionally used to treat malaria in western part of Nigeria by evaluating its antiplasmodial efficacies. A maceration of dried leaves ofL. taraxacifolia was prepared in ethanol. Alkaloid and terpenoid fractions ofL. taraxacifolia were extracted using solvent-solvent extraction methods. Antiplasmodial activities of crude extract, alkaloid and terpenoid fractions ofL. taraxacifolia were assessed by suppressive, ...
Source: Comparative Clinical Pathology - August 15, 2020 Category: Pathology Source Type: research

Altered blood oxidative stress markers in association with antioxidant supplemented therapy for mange, tick, and flea allergic dermatitis of dogs
AbstractSkin diseases are among the most common health problems in dogs. The study aimed to evaluate the oxidative stress biomarker profile in mange-, tick-, and flea-infested dogs and to assess the value of antioxidant supplementation as complementary therapy to standard treatment. Thirty-seven dogs were enrolled in the study, and they were divided into three groups: apparently healthy group (11 dogs), mange group (8 dogs), and tick- and flea-infested group (18 dogs). Four dogs out of mange group and eleven dogs out of tick- and flea-infested group were treated with specific therapy supplemented with vitamin E and seleniu...
Source: Comparative Clinical Pathology - August 14, 2020 Category: Pathology Source Type: research

Comparative study of hematological profile of three forage fish species habiting in Dal Lake of Kashmir Himalaya, India
AbstractThe forage fish species are considered as a vital source of food for other predators and are also being used as dry human food in the Kashmir valley during the winter season. However, due to disturbance in their habitat, the population of the fishes under study,Triplophysa kashmirensis,Pethia conchonius andCrossocheilus diplochilus has also declined and needs attention for their well-being. Therefore, the present study was carried out with an aim to evaluate the hematological profile of these three forage fishes habiting in Dal lake of Kashmir Himalaya. Live adult fish specimens were collected from Dal Lake with th...
Source: Comparative Clinical Pathology - August 12, 2020 Category: Pathology Source Type: research

Hepatic, renal, and pancreatic damage associated with chronic exposure to oral and inhaled 2,4-dichlorophenoxy acetic acid (2,4-d): an environmental exposure model in rats
Abstract2,4-Diclophenoxyacetic acid (2,4-D) is one of the most widely used herbicides in the world. The aim of this study was to evaluate the possible toxic effect of chronic exposure to oral and inhaled 2,4-D herbicide on the liver, kidney, and pancreas by simulating environmental exposure. Eighty male albino adult Wistar rats divided into eight groups were exposed for 6 months to inhaled and oral (contaminated feed) three different doses of 2,4-D [3.71 × 10−3 g of active ingredient per hectare (g a.i./ha), 6.19 × 10−3 g a.i./ha and 9.28 × 10−3 g a.i./ha]. Blood, liver, kidney, spleen, ...
Source: Comparative Clinical Pathology - August 11, 2020 Category: Pathology Source Type: research

MDS/MPN with ringed sideroblasts and thrombocytosis, and secondary or concurrent mastocytosis: case report and literature review
We present this case to point out a rare combination of systemic mastocytosis and MDS/MPN with ring sideroblasts and thrombocytosis. It is not known if the best treatment should be focused on the systemic mastocytosis or the accompanying MDS/MPN. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 31, 2020 Category: Pathology Source Type: research

Comparative study on the effects of Alchornea cordifolia and Cassia spectabilis leaf extracts on diclofenac-induced liver and kidney injuries in rats
AbstractHerbal extracts may be useful in the management of hepatic and renal diseases in humans. Thus, this study investigated the effects ofAlchornea cordifolia (ACE) andCassia spectabilis (CSE) leaf extracts on diclofenac-induced hepatorenal injuries in rats and compared their activities. Adult rats were selected into four groups consisting of 5 rats each. The rats in groups 1 and 2 received normal saline and 11.0  mg/kg diclofenac sodium (DF) by intramuscular route for 5 days respectively. The groups 3 and 4 rats were given 342 mg/kg ACE and CSE respectively by oral gavages for 6 days, after DF expos...
Source: Comparative Clinical Pathology - July 30, 2020 Category: Pathology Source Type: research

The effect of cinnamic acid on fetal hippocampus in pregnant rats
In this study, the researchers examined the neuroprotective effect of CA on the parameters of oxidative stress and hippocampal cell density in the UPI rat model. In this experiment, the Wistar rats were mated, and UPI was induced at gestation day (GD) 18. For UPI induction, anterior uterine artery occlusion surgery is conducted in deep anesthesia. From GD15, CA was administrated orally in 25, 50, and 100  mg/kg BW doses until GD21. GD21 evaluated the weight of the uterine, placenta, and embryo. Also, the oxidative stress parameters in the hippocampus, e.g., catalase activity (CAT), total antioxidant activity (TAC), an...
Source: Comparative Clinical Pathology - July 24, 2020 Category: Pathology Source Type: research

Role of Vaccinium arctostaphylos extract on CCl4-induced chronic liver fibrosis in rats
AbstractLiver fibrosis is an initial stage of cirrhosis being a result of the pathological deposition of the extracellular matrix. Plants, present a worthwhile source for new drug finding because of a remarkable chemical variety of compounds existing within.Vaccinium arctostaphylos (Va) fruit contains many bioactive anthocyanins that have powerful antioxidant, anti-inflammatory, and anti-tumor properties and traditionally are used to control hypertension and diabetes mellitus in Iran. The current study was established to evaluate the therapeutic effects of Va in chronic liver fibrosis. Liver fibrosis was induced by carbon ...
Source: Comparative Clinical Pathology - July 24, 2020 Category: Pathology Source Type: research

Zingerone ameliorates gentamicin-induced nephrotoxicity in rats
AbstractGentamicin (GEN) is an antibiotic to treat severe gram-negative infections. Nephrotoxicity is the major adverse effect limiting clinical use of GEN. Zingerone (ZIN), an important component of ginger root, has several pharmacological effects including antioxidant, anti-inflammatory, and free radical scavenging activity. The present study investigated the effects of ZIN against GEN-induced nephrotoxicity. Twenty-eight rats were randomly divided into four groups: group I received normal saline, group II was treated with GEN (100  mg/kg/day, i.p.), group III was treated with ZIN (10 mg/kg/day, orally) and GEN...
Source: Comparative Clinical Pathology - July 23, 2020 Category: Pathology Source Type: research

Sensitivities, specificities and correlates of certain liver damage marker parameters in Red Sokoto goats
This study comparatively evaluated the sensitivity and specificity of various serum biochemical markers of liver damage in the diagnosis of hepatic disorders in Red Sokoto goats billed for slaughter. The study design was a cross-sectional survey. Research visits to Ikpa Market Lairage and Abattoir located in Nsukka, Enugu state, Nigeria, were made two times a week for the period of 12  weeks. All goats presented for sale and slaughter on the days of the research visits were evaluated. The goats were examined and tagged. Blood samples were collected from the jugular veins of the goats prior to slaughter for harvesting ...
Source: Comparative Clinical Pathology - July 22, 2020 Category: Pathology Source Type: research

Modulatory effects of melatonin on erythrocyte indices in Arabian stallions following a 30-km sub-maximal exercise
AbstractExperiments were performed to determine the effects of melatonin on erythrocytes indices in Arabian stallions following a sub-maximal exercise of 30  km. Twelve, apparently, healthy stallions of average age 6.3 ± 4.4 years and mean weight 403.44 ± 12.46 kg were used. They were divided into two groups of six stallions each. The stallions in MT which was the experimental group were administered with melatonin before exercise while sta llions in the second group (NT) which served as the control were not administered with melatonin. Erythrocyte count in the MT gr...
Source: Comparative Clinical Pathology - July 19, 2020 Category: Pathology Source Type: research

Existence of immune cells in uterine luminal flush of repeat breeding Egyptian buffalo-cow ( Bubalus bubalis ) : salpingitis and endometritis as a major cause
AbstractThe aim of this study was to investigate the existence of immune cells in uterine flush of repeat breeding Egyptian buffalo-cow (Bubalus bubalis) during follicular and luteal stages of estrous cycle. Furthermore, histopathological examination of fallopian tubes and uteri was conducted. A total of 30 reproductive tracts of buffalo-cow were obtained from a local slaughterhouse directly aft er slaughter. Collected genital tracts were transported to the laboratory within 1.5  h. Samples of uterine fluids were collected, centrifuged, stained and used for quantification of immune cells during follicular and luteal s...
Source: Comparative Clinical Pathology - July 15, 2020 Category: Pathology Source Type: research

In silico analysis of a chimeric fusion protein as a new vaccine candidate against Clostridium perfringens type A and Clostridium septicum alpha toxins
AbstractIn silico analysis is the most important approach to understand protein structure and functions, and the most important problem for designing and producing a fusion construct is producing large amounts of functional protein.Clostridium perfringens type A andClostridium septicum produce alpha (plc) and alpha toxins respectively.C. perfringens can cause gas gangrene and gastrointestinal diseases.C. septicum can cause traumatic and non-traumatic gas gangrene. The aim of current research was in silico analysis of a chimeric fusion protein againstC. perfringens type A andC. septicum alpha toxins. Firstly, the chimeric f...
Source: Comparative Clinical Pathology - July 13, 2020 Category: Pathology Source Type: research

Sub-chronic administration of flavonoid fraction Daflon improve lead-induced alterations in delta-aminolevulinic acid dehydratase activity, erythrocytic parameters, and erythrocyte osmotic fragility in Wistar rats
This study investigated the ameliorative potential of micronized purified flavonoid fraction Daflon on lead-induced alterations in heme-biosynthetic enzyme delta-aminolevulinic acid dehydratase ( δ-ALAD) activity and erythrocytic parameters. Thirty-two male adult Wistar rats were divided by simple randomization into four groups of eight rats each. Lead acetate was administered orally at a dose of 190 mg/kg (1/20th of the LD50) body weight for 42  days, either alone or with Daflon at a dose of 100 mg/kg body weight. Blood lead level (BLL) was analyzed using the microwave plasma atomic emission spectrome...
Source: Comparative Clinical Pathology - July 12, 2020 Category: Pathology Source Type: research

Effect of molybdenum trioxide nanoparticles on histological changes of uterus and biochemical parameters of blood serum in rat
The objective of this study was the evaluation of its effects on uterus and biochemistry of blood serum in rat. A total number of 42 adult female Wistar rats were divided into six groups of seven animals each. The groups included one control group, four experimental groups, and one sham group. The control group received no nanoparticle. The experimental groups were treated with 50, 100, 200, and 300  mg/kg of MoO3, and for the sham group, physiological serum was injected intraperitoneally. Thirty-five days after the treatment, blood samples were taken; the uterus and uterine tubes were removed and fixed in 10% formali...
Source: Comparative Clinical Pathology - July 12, 2020 Category: Pathology Source Type: research

Prediction of serum biochemistry of pigs at pre- and post-weaning stages of growth using regression tree methods
This study was aimed at evaluating the effects of breed, sex and their interactions on aspects of haematology, serum biochemistry and morphometry of healthy pigs at pre- and post-weaning stages of growth (4 and 12  weeks respectively). It was also aimed at predicting the serum biochemical parameters (SBP) that are biomarkers for growth using regression tree method (RTM). Seventy-one crossbred (71 CB) pigs (Landrace × Local pigs) and 21 Local (L) pigs were reared from birth to adult stage, with blood samples and morphometric parameters (MP) taken at pre- and post-weaning stages. The CB pigs had higher values for ...
Source: Comparative Clinical Pathology - July 6, 2020 Category: Pathology Source Type: research

Renoprotective property of the flavonoid-rich extract of Kigelia africana fruits on gentamicin-induced nephrotoxicity in rats
In this study, the flavonoid-rich extract ofKigelia africana fruits (FRE) was investigated for protective property against gentamicin-induced nephrotoxicity in Wistar rats. Animals were divided into eight groups (n = 6): group 1 (control) received distilled water only. Group 2 received gentamicin (60 mg/kg/i.p). Groups 3 received rutin (100 mg/kg/p.o) and gentamicin (60 mg/kg/i.p). Groups 4–5 received FRE (100 and 200 mg/kg/p.o) and gentamicin (60 mg/kg/i.p), respectively. Group 6 received rutin (1 00 mg/kg/p.o) alone. Groups 7–8 received FRE (100 and 200 mg/kg/p...
Source: Comparative Clinical Pathology - June 28, 2020 Category: Pathology Source Type: research

Antiplasmodial activity of total alkaloids and flavonoids of stem bark extracts of Enantia chlorantha in mice
AbstractPlant-derived compounds have played vital roles in the management of malaria infections. Thus,Enantia chlorantha (Anonaceae) is one of such plants used in folklore medicine in Nigeria for the treatment of malaria. There is, therefore, a need to validate the potential in this plant by investigating its in vivo antiplasmodial activity againstPlasmodium berghei-infected mice. The alkaloid and flavonoid extracts were extracted from the crude extract following standard procedure. The crude extract was orally administered toPlasmodium berghei-infected mice at 200, 400 and 800  mg/kg bodyweight (kgbw), while flavonoi...
Source: Comparative Clinical Pathology - June 26, 2020 Category: Pathology Source Type: research

Impact of IL-10 gene promoter -1082 (G/A) polymorphism in B cell chronic lymphocytic leukemia
This study aimed to study the relation between IL-10 (rs1800896)-1082 (G/A) gene polymorphism and the risk of development of B cell chronic lymphocytic leukemia and its impact on the patient outcome. IL-10 (rs1800896)-1082 (G/A) gene polymorphism was assessed in fifty patients with chronic lymphocytic leukemia as well as 50 age and sex-matched apparently healthy controls using polymerase chain reaction (PCR). The IL-10 (rs1800896) AG hetero, GG homo, AG + GG genotypes, and G allele showed significantly higher frequency (P >  0.001) in CLL cases when compared to control groups. The genotype AG + GG were sig...
Source: Comparative Clinical Pathology - June 26, 2020 Category: Pathology Source Type: research

Ameliorative effect of Gongronema latifolium leaf extract on alloxan-induced diabetic cardiomyopathy in Wistar rats model
This study was designed to investigate the effect ofGongronema latifolium leaf extract on DCM in alloxan-induced diabetic rats. Alloxan monohydrate was used to induce diabetes by an intraperitoneal injection of (150  mg/kg). Three diabetic groups were administered aqueous leaf extract ofGongronema latifolium at 6.36, 12.72, and 25.44  mg/kg bodyweight (BW) respectively; a group was administered with metformin (5 mg/kg BW), while the other two served as positive and negative control. Thereafter, fasting blood glucose, antioxidant enzymes, malondialdehyde level, serum lactate dehydrogenase, creatine kinase-MB,...
Source: Comparative Clinical Pathology - June 17, 2020 Category: Pathology Source Type: research

Serum symmetric dimethylarginine levels in a half-breed German shepherd dog with renal cystadenocarcinoma and nodular dermatofibrosis
This report describes the diagnosis, clinical signs, and laboratory and histopathological findings of a canine renal cystadenocarcinoma and nodular dermatofibrosis. A 10-year-old entire female half-breed German Shepherd Dog x Rottweiler was brought to a veterinary clinic with hematuria and was non-responsive to the treatment with ciprofloxacin and prednisone. Erythrogram showed a normocytic normochromic anemia. Serum biochemical analysis revealed mildly elevated aspartate aminotransferase activity and high level of symmetric dimethylarginine. Data acquired during physical examination, abdominal sonography and X-ray evaluat...
Source: Comparative Clinical Pathology - June 17, 2020 Category: Pathology Source Type: research

Correction to: Main compounds and in vitro effectiveness of Syzygium aromaticum essential oil on protoscoleces of hydatid cyst
In the original version of this article, the first name of the corresponding author Sidi was incorrectly spelled as “Sid”. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 8, 2020 Category: Pathology Source Type: research

Metabolic enzyme profile, behavioural changes and morphophysiological parameters of African catfish Clarias gariepinus juveniles in response to burnt waste tyres
AbstractWater-soluble fractions (WSFs) of waste burnt tyres (WBTs) were tested on enzymatic activities, behavioural changes and histology of gills and liver of African catfishClarias gariepinus juveniles for 96  h at 5.00, 10.00, 15.00, 20.00 and 25.00 g/L in a non renewable static bioassay. Water quality parameters of the experimental media showed significant (p 
Source: Comparative Clinical Pathology - June 8, 2020 Category: Pathology Source Type: research

Effect of post-prandial lipemia on canine biochemical parameters
This study assessed postprandial lipemia of healthy dogs fed with commercial feed, as well as the effect of lipemia induced in vitro by the addition of commercial lipid solution. Twenty healthy dogs were selected, for induction of postprandial lipemia, and the peak lipemic time (3 h after feeding) was compared with two non-lipemic times (12 h of fasting), 1 day before and 1 day after the induction of lipemia, respectively. Lipid solution was added to non-lipemic sera simulating triglyceride concentrations similar to the minimum, medium, and maximum values obtained at the lipemic time in vivo. Biochemical analyses were perf...
Source: Comparative Clinical Pathology - June 4, 2020 Category: Pathology Source Type: research

Retrospective analysis of globule leukocytes in parasite-free rumens of cattle: a histopathological research with literature review
AbstractHaving previously been described by several researchers, an association of globule leukocytes with helminth infestation involving the gastrointestinal tracts has been well acknowledged in humans and animals; however, the appearance of globule leukocytes is less common in those disorders which are not associated with parasitism. It has been suggested that the appearance of globule leukocytes, which are probably derived from mast cells, may indicate a significant role in immune response. The present retrospective study of archived bovine cases with postmortem diagnosis of rumen disorder that was not associated with p...
Source: Comparative Clinical Pathology - May 31, 2020 Category: Pathology Source Type: research

Comparative evaluation of hematobiochemical effects of caudal epidural administration of detomidine, lidocaine, and lidocaine/detomidine in dromedary calf camels
AbstractThe present study aims to comparatively evaluate the effects of caudal epidural detomidine, lidocaine, and lidocaine/detomidine on hematobiochemical parameters in dromedary calf camels. A total of ten male dromedary calf camels (Camelus dromedarius) with the age of 20  ± 2 weeks and weight of 85 ± 5.5 kg were used for the study. The animals were enrolled to five groups (4 experimental and 1 control group) in a randomized, crossover Latin square design, with a minimum washout period of 7 days between treatments. Calves in groups DL and DH received epi dural lo...
Source: Comparative Clinical Pathology - May 31, 2020 Category: Pathology Source Type: research

Pathophysiology of STZ-induced pancreatic β cell injury and dysfunction: traditional role of Boswellia ovalifoliolata Bal. & amp; Henry on diabetes and dyslipidemia
This study was aimed to investigate the antidiabetic and antihyperlipidemic activities of aqueous extract of stem bark ofBoswellia ovalifoliolata (AESBBO). The phytochemical analysis of various extracts revealed the presence of flavonoids, glycosides, steroids, triterpenes, saponins, carbohydrates, alkaloids, and tannins. Either hexane, ethyl acetate, and methanol extracts did not produce any antidiabetic activity but aqueous extract showed that a significant antidiabtic activity could be due to presence of phytoconstituents like flavonoids, tannins, carbohydrates, and saponins in aqueous extract. The diabetic rats were tr...
Source: Comparative Clinical Pathology - May 31, 2020 Category: Pathology Source Type: research

Diagnostic value of CD64 in early detection of neonatal sepsis
This study included 74 neonates divided into three groups: group 1, documented sepsis (26 patients with positive bacterial culture); group 2, clinical sepsis (25 patients without culture-proven sepsis with the presence of two or more clinical symptoms or signs of sepsis); and group 3, control group (twenty-three healthy neonates). CD64 was measured at admission using enzyme-linked immunosorbent assay (ELISA) kits. A cutoff value of ≥ 535.6 pg/dl can predict neonatal sepsis with 96.1% sensitivity and 91.3% specificity. Serum CD64 was significantly higher in documented and clinical sepsis groups than in contro...
Source: Comparative Clinical Pathology - May 31, 2020 Category: Pathology Source Type: research

Intake of snacks containing curcumin stimulates erythropoiesis and antioxidant response in dogs
The objective of this study was to determine whether snacks containing curcumin have beneficial effects on dog health. The snacks were produced from commercial canned meat for dogs, and curcumin was added and homogenized. Snacks containing 15  mg of curcumin were produced, frozen, and offered to dogs twice a day. Ten beagles (6 months of age) were used. The animals were placed in an experimental kennel and allocated randomly to 1 of 2 treatments (5 dogs/treatment): snacks containing curcumin (curcumin; 30 mg of curcumin/animal/day) or not (control). On day 15, numbers of erythrocytes and hematocrit were grea...
Source: Comparative Clinical Pathology - May 31, 2020 Category: Pathology Source Type: research

Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report
This report describes the clinical presentation, pathology and molecular diagnosis of canine parvovirus infection in male Boerboel and female Alsatian puppies. The history of the dogs was considered, examined clinically for vital parameters, haemogram changes and faeces screened for parasites and canine parvovirus faecal antigen. Tissue samples were taken at necropsy for confirmatory diagnosis using histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. There was a severe regenerative anaemia, leucopenia and lymphopaenia. The positive antigen faecal test and pathological findings of ha...
Source: Comparative Clinical Pathology - May 27, 2020 Category: Pathology Source Type: research

Effect of oral melatonin administration on inflammatory cytokines and acute phase proteins after the castration of dogs
AbstractMelatonin has been used perioperative to control pain, inflammation, and oxidative stress. The present paper aims to evaluate the effect of the administration of melatonin on acute phase proteins and inflammatory cytokines and compare the trends after the melatonin administration in intact and castrated dogs. Twenty healthy mature male dogs (1 –3 years old) were divided randomly into 4 groups (n = 5): melatonin, castrated and melatonin, castrated, and control. Melatonin (3 mg capsule/10 kg) was administrated every 24 h for 1 month and castration was performed on day 0. B...
Source: Comparative Clinical Pathology - May 27, 2020 Category: Pathology Source Type: research

Protective and therapeutic effects of Phoenix dactylifera leaf extract on pancreatic β-cells and diabetic parameters in streptozotocin-induced diabetic rats
In this study, diabetes was induced in male Wistar rats via intraperitoneal administration of STZ (55 mg/kg). In this study, 49 rats were divided into seven groups randomly (n = 7 per group). Our three treatment groups for 42 days were given 250 mg/kg ofP.d extract, 500  mg/kg ofP.d extract, and 5  mg/kg glibenclamide orally, respectively. Then, the animals were anesthetized and blood samples were obtained from puncturing the retro-orbital sinus at the initial and end of the experiment and finally sacrificed. Fasting blood glucose (FBG) levels and serum biochemical parameters includin...
Source: Comparative Clinical Pathology - May 26, 2020 Category: Pathology Source Type: research