Renoprotective property of the flavonoid-rich extract of Kigelia africana fruits on gentamicin-induced nephrotoxicity in rats
In this study, the flavonoid-rich extract ofKigelia africana fruits (FRE) was investigated for protective property against gentamicin-induced nephrotoxicity in Wistar rats. Animals were divided into eight groups (n = 6): group 1 (control) received distilled water only. Group 2 received gentamicin (60 mg/kg/i.p). Groups 3 received rutin (100 mg/kg/p.o) and gentamicin (60 mg/kg/i.p). Groups 4–5 received FRE (100 and 200 mg/kg/p.o) and gentamicin (60 mg/kg/i.p), respectively. Group 6 received rutin (1 00 mg/kg/p.o) alone. Groups 7–8 received FRE (100 and 200 mg/kg/p...
Source: Comparative Clinical Pathology - June 29, 2020 Category: Pathology Source Type: research

Antiplasmodial activity of total alkaloids and flavonoids of stem bark extracts of Enantia chlorantha in mice
AbstractPlant-derived compounds have played vital roles in the management of malaria infections. Thus,Enantia chlorantha (Anonaceae) is one of such plants used in folklore medicine in Nigeria for the treatment of malaria. There is, therefore, a need to validate the potential in this plant by investigating its in vivo antiplasmodial activity againstPlasmodium berghei-infected mice. The alkaloid and flavonoid extracts were extracted from the crude extract following standard procedure. The crude extract was orally administered toPlasmodium berghei-infected mice at 200, 400 and 800  mg/kg bodyweight (kgbw), while flavonoi...
Source: Comparative Clinical Pathology - June 27, 2020 Category: Pathology Source Type: research

Impact of IL-10 gene promoter -1082 (G/A) polymorphism in B cell chronic lymphocytic leukemia
This study aimed to study the relation between IL-10 (rs1800896)-1082 (G/A) gene polymorphism and the risk of development of B cell chronic lymphocytic leukemia and its impact on the patient outcome. IL-10 (rs1800896)-1082 (G/A) gene polymorphism was assessed in fifty patients with chronic lymphocytic leukemia as well as 50 age and sex-matched apparently healthy controls using polymerase chain reaction (PCR). The IL-10 (rs1800896) AG hetero, GG homo, AG + GG genotypes, and G allele showed significantly higher frequency (P >  0.001) in CLL cases when compared to control groups. The genotype AG + GG were sig...
Source: Comparative Clinical Pathology - June 27, 2020 Category: Pathology Source Type: research

Ameliorative effect of Gongronema latifolium leaf extract on alloxan-induced diabetic cardiomyopathy in Wistar rats model
This study was designed to investigate the effect ofGongronema latifolium leaf extract on DCM in alloxan-induced diabetic rats. Alloxan monohydrate was used to induce diabetes by an intraperitoneal injection of (150  mg/kg). Three diabetic groups were administered aqueous leaf extract ofGongronema latifolium at 6.36, 12.72, and 25.44  mg/kg bodyweight (BW) respectively; a group was administered with metformin (5 mg/kg BW), while the other two served as positive and negative control. Thereafter, fasting blood glucose, antioxidant enzymes, malondialdehyde level, serum lactate dehydrogenase, creatine kinase-MB,...
Source: Comparative Clinical Pathology - June 18, 2020 Category: Pathology Source Type: research

Serum symmetric dimethylarginine levels in a half-breed German shepherd dog with renal cystadenocarcinoma and nodular dermatofibrosis
This report describes the diagnosis, clinical signs, and laboratory and histopathological findings of a canine renal cystadenocarcinoma and nodular dermatofibrosis. A 10-year-old entire female half-breed German Shepherd Dog x Rottweiler was brought to a veterinary clinic with hematuria and was non-responsive to the treatment with ciprofloxacin and prednisone. Erythrogram showed a normocytic normochromic anemia. Serum biochemical analysis revealed mildly elevated aspartate aminotransferase activity and high level of symmetric dimethylarginine. Data acquired during physical examination, abdominal sonography and X-ray evaluat...
Source: Comparative Clinical Pathology - June 18, 2020 Category: Pathology Source Type: research

Correction to: Main compounds and in vitro effectiveness of Syzygium aromaticum essential oil on protoscoleces of hydatid cyst
In the original version of this article, the first name of the corresponding author Sidi was incorrectly spelled as “Sid”. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 9, 2020 Category: Pathology Source Type: research

Metabolic enzyme profile, behavioural changes and morphophysiological parameters of African catfish Clarias gariepinus juveniles in response to burnt waste tyres
AbstractWater-soluble fractions (WSFs) of waste burnt tyres (WBTs) were tested on enzymatic activities, behavioural changes and histology of gills and liver of African catfishClarias gariepinus juveniles for 96  h at 5.00, 10.00, 15.00, 20.00 and 25.00 g/L in a non renewable static bioassay. Water quality parameters of the experimental media showed significant (p 
Source: Comparative Clinical Pathology - June 9, 2020 Category: Pathology Source Type: research

Effect of post-prandial lipemia on canine biochemical parameters
This study assessed postprandial lipemia of healthy dogs fed with commercial feed, as well as the effect of lipemia induced in vitro by the addition of commercial lipid solution. Twenty healthy dogs were selected, for induction of postprandial lipemia, and the peak lipemic time (3 h after feeding) was compared with two non-lipemic times (12 h of fasting), 1 day before and 1 day after the induction of lipemia, respectively. Lipid solution was added to non-lipemic sera simulating triglyceride concentrations similar to the minimum, medium, and maximum values obtained at the lipemic time in vivo. Biochemical analyses were perf...
Source: Comparative Clinical Pathology - June 5, 2020 Category: Pathology Source Type: research

Retrospective analysis of globule leukocytes in parasite-free rumens of cattle: a histopathological research with literature review
AbstractHaving previously been described by several researchers, an association of globule leukocytes with helminth infestation involving the gastrointestinal tracts has been well acknowledged in humans and animals; however, the appearance of globule leukocytes is less common in those disorders which are not associated with parasitism. It has been suggested that the appearance of globule leukocytes, which are probably derived from mast cells, may indicate a significant role in immune response. The present retrospective study of archived bovine cases with postmortem diagnosis of rumen disorder that was not associated with p...
Source: Comparative Clinical Pathology - June 1, 2020 Category: Pathology Source Type: research

Comparative evaluation of hematobiochemical effects of caudal epidural administration of detomidine, lidocaine, and lidocaine/detomidine in dromedary calf camels
AbstractThe present study aims to comparatively evaluate the effects of caudal epidural detomidine, lidocaine, and lidocaine/detomidine on hematobiochemical parameters in dromedary calf camels. A total of ten male dromedary calf camels (Camelus dromedarius) with the age of 20  ± 2 weeks and weight of 85 ± 5.5 kg were used for the study. The animals were enrolled to five groups (4 experimental and 1 control group) in a randomized, crossover Latin square design, with a minimum washout period of 7 days between treatments. Calves in groups DL and DH received epi dural lo...
Source: Comparative Clinical Pathology - June 1, 2020 Category: Pathology Source Type: research

Pathophysiology of STZ-induced pancreatic β cell injury and dysfunction: traditional role of Boswellia ovalifoliolata Bal. & amp; Henry on diabetes and dyslipidemia
This study was aimed to investigate the antidiabetic and antihyperlipidemic activities of aqueous extract of stem bark ofBoswellia ovalifoliolata (AESBBO). The phytochemical analysis of various extracts revealed the presence of flavonoids, glycosides, steroids, triterpenes, saponins, carbohydrates, alkaloids, and tannins. Either hexane, ethyl acetate, and methanol extracts did not produce any antidiabetic activity but aqueous extract showed that a significant antidiabtic activity could be due to presence of phytoconstituents like flavonoids, tannins, carbohydrates, and saponins in aqueous extract. The diabetic rats were tr...
Source: Comparative Clinical Pathology - June 1, 2020 Category: Pathology Source Type: research

Diagnostic value of CD64 in early detection of neonatal sepsis
This study included 74 neonates divided into three groups: group 1, documented sepsis (26 patients with positive bacterial culture); group 2, clinical sepsis (25 patients without culture-proven sepsis with the presence of two or more clinical symptoms or signs of sepsis); and group 3, control group (twenty-three healthy neonates). CD64 was measured at admission using enzyme-linked immunosorbent assay (ELISA) kits. A cutoff value of ≥ 535.6 pg/dl can predict neonatal sepsis with 96.1% sensitivity and 91.3% specificity. Serum CD64 was significantly higher in documented and clinical sepsis groups than in contro...
Source: Comparative Clinical Pathology - June 1, 2020 Category: Pathology Source Type: research

Intake of snacks containing curcumin stimulates erythropoiesis and antioxidant response in dogs
The objective of this study was to determine whether snacks containing curcumin have beneficial effects on dog health. The snacks were produced from commercial canned meat for dogs, and curcumin was added and homogenized. Snacks containing 15  mg of curcumin were produced, frozen, and offered to dogs twice a day. Ten beagles (6 months of age) were used. The animals were placed in an experimental kennel and allocated randomly to 1 of 2 treatments (5 dogs/treatment): snacks containing curcumin (curcumin; 30 mg of curcumin/animal/day) or not (control). On day 15, numbers of erythrocytes and hematocrit were grea...
Source: Comparative Clinical Pathology - June 1, 2020 Category: Pathology Source Type: research

Pathology and molecular diagnosis of canine parvoviral enteritis in Nigeria: case report
This report describes the clinical presentation, pathology and molecular diagnosis of canine parvovirus infection in male Boerboel and female Alsatian puppies. The history of the dogs was considered, examined clinically for vital parameters, haemogram changes and faeces screened for parasites and canine parvovirus faecal antigen. Tissue samples were taken at necropsy for confirmatory diagnosis using histopathology, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. There was a severe regenerative anaemia, leucopenia and lymphopaenia. The positive antigen faecal test and pathological findings of ha...
Source: Comparative Clinical Pathology - May 28, 2020 Category: Pathology Source Type: research

Effect of oral melatonin administration on inflammatory cytokines and acute phase proteins after the castration of dogs
AbstractMelatonin has been used perioperative to control pain, inflammation, and oxidative stress. The present paper aims to evaluate the effect of the administration of melatonin on acute phase proteins and inflammatory cytokines and compare the trends after the melatonin administration in intact and castrated dogs. Twenty healthy mature male dogs (1 –3 years old) were divided randomly into 4 groups (n = 5): melatonin, castrated and melatonin, castrated, and control. Melatonin (3 mg capsule/10 kg) was administrated every 24 h for 1 month and castration was performed on day 0. B...
Source: Comparative Clinical Pathology - May 28, 2020 Category: Pathology Source Type: research

Protective and therapeutic effects of Phoenix dactylifera leaf extract on pancreatic β-cells and diabetic parameters in streptozotocin-induced diabetic rats
In this study, diabetes was induced in male Wistar rats via intraperitoneal administration of STZ (55 mg/kg). In this study, 49 rats were divided into seven groups randomly (n = 7 per group). Our three treatment groups for 42 days were given 250 mg/kg ofP.d extract, 500  mg/kg ofP.d extract, and 5  mg/kg glibenclamide orally, respectively. Then, the animals were anesthetized and blood samples were obtained from puncturing the retro-orbital sinus at the initial and end of the experiment and finally sacrificed. Fasting blood glucose (FBG) levels and serum biochemical parameters includin...
Source: Comparative Clinical Pathology - May 27, 2020 Category: Pathology Source Type: research

Effect of the aqueous root extract of Curcuma longa L. (turmeric) against thermally oxidized oil-induced hematological, biochemical and histopathological alterations
This study was performed to evaluate the role of usingCurcuma longa L. (turmeric) against thermally oxidized oil-induced hematological, biochemical, and histopathological alterations. Eighty male rabbits were randomly divided into 4 groups (20 of each) and treated for 90  days as follows: The first one was kept as standard control group. Groups 2 and 3 receivedCurcuma longa (CL) 13  g/kg, 5% thermally oxidized oil to ration separately, where the last group was supplemented by CL together with the thermally oxidized oil. Blood samples were collected via ear veins from all rabbits for hematological and bi...
Source: Comparative Clinical Pathology - May 27, 2020 Category: Pathology Source Type: research

Hepatocurative effect of aqueous extract of Scoparia dulcis (sweet broom) root on carbon tetrachloride-induced hepatotoxicity in albino rats
AbstractMost acute and chronic liver diseases today are due to drugs and other chemical-induced hepatotoxicity. A great number of herbal preparations have become increasingly popular for the treatment of liver disorders due to the absence of reliable hepatoprotective drugs in modern medicine. The aim of this work is to carry out acute toxicity study and investigate the hepatocurative effects of aqueous root extract ofScoparia dulcis against carbon tetrachloride (CCl4)-induced hepatoxicity in albino rats. Serum biomarkers of hepatic injury, alanine aminotransferase (ALT), aspartate amino transferase (AST), alkaline phosphat...
Source: Comparative Clinical Pathology - May 25, 2020 Category: Pathology Source Type: research

Diagnosis of mange in West African Dwarf (WAD) and Red Sokoto (RS) goats
AbstractThe diagnosis of mange is problematic in the absence of mites in skin scrapings because many skin diseases present similar gross features as mange. The study evaluated the stepwise procedure for herd diagnosis of mange in the West African Dwarf (WAD) and Red Sokoto (RS) goats with greater specificity and sensitivity. Twenty-seven WAD goats (n = 27) and forty RS goats (n = 40) goats with mange-like skin lesions (alopecia, scabs, crusts, and scratch injuries) out of a total herd population of 114 WAD goats and 317 RS goats respectively in Nsukka, Eastern Nigeria, were examined by directed ...
Source: Comparative Clinical Pathology - May 24, 2020 Category: Pathology Source Type: research

Main compounds  and in vitro effectiveness of Syzygium aromaticum essential oil on protoscoleces of hydatid cyst
AbstractSurgery remains the preferred treatment for cystic echinococcosis. On the other hand, one of the side effects of hydatid cyst surgery is recurrence. Many scolicidal agents have been used for inactivation of the hydatid cyst content. However, many of these scolicidal agents may cause undesirable complications that limit their usage. The aims of this study are to investigate the chemical composition and in vitro scolicidal effect ofS. aromaticum essential oil against protoscoleces from hydatid cysts ofEchinococcus granulosus. The essential oil was obtained by hydrodistillation. Gas chromatography with flame ionizatio...
Source: Comparative Clinical Pathology - May 23, 2020 Category: Pathology Source Type: research

Evaluation of the hepatoprotective effect of oral administration of aqueous fraction of methanolic extract of Costus afer leaves during induction of hepatocellular carcinoma with diethylnitrosamine in rats
This study evaluated the hepatoprotective effect of oral administration of aqueous fraction of methanolic extract ofCostus afer leaves (CALAF) during induction of hepatocellular carcinoma (HCC) with diethylnitrosamine (DEN) in rats. The methanolic leaf extract was fractionated into hexane, ethyl acetate, butanol, and aqueous fractions. The in vitro antioxidant potential of the fractions were estimated by the assays of 2,2-diphenyl-1-picrylhydrazine and nitric oxide radical scavenging activity, ferric-reducing antioxidant potential, and total antioxidant capacity. CALAF had the most antioxidant effect. Rats were orally pret...
Source: Comparative Clinical Pathology - May 22, 2020 Category: Pathology Source Type: research

Changes of acute-phase proteins during different physiological conditions in dairy Saanen goats
This study was aimed to investigate the changes of acute-phase proteins (APPs) during important physiological condition (late pregnancy and early lactation) in dairy Saanen goats. Twenty clinically healthy Saanen does with an age of 3  ± 0.5 years were used for the study. They were randomly selected from a larger herd according to singleton pregnancy. Samples were collected from jugular vein at three different physiological times which included T1, non-pregnancy non-lactation (before pregnancy); T2, late pregnancy (4-month pregnancy); and T3, early lactation (1 month after parturition). The a...
Source: Comparative Clinical Pathology - May 22, 2020 Category: Pathology Source Type: research

Clinical and pathological responses of broilers to ocular vaccination using plant gum delivery and challenge with infectious bursal disease virus
This study therefore evaluates the changes in clinical, haematological and histopathologcal responses of broilers vaccinated with infectious bursal disease virus (IBDV) vaccine, administered with theCedrela odorata andKhaya senegalensis as phytogenic mucoadhesive following experimental infection with virulent IBDV. Two hundred (200)-day-old birds were divided into four groups: as combined gums-IBDV vaccine (A), IBDV vaccine alone (B), gums alone (C) and no gum not vaccinated (D), respectively. The IBDV vaccination was administered ocularly at 14 and 28  days and later challenged with the virulent IBDV at 21 days....
Source: Comparative Clinical Pathology - May 20, 2020 Category: Pathology Source Type: research

Serum concentration of 25 (OH) vitamin D in healthy dogs: factors as age, sex, and diet
This study aimed at determining serum 25(OH)D values in healthy dogs and evaluating its association with factors such as sex, age, and diet. Blood samples were collected from healthy intact male and female dogs divided into two groups: (G1) 30 dogs (Beagles), 15 adults and 15 puppies, receiving standardized diet; (G2) 29 dogs of different breeds, adults, receiving varied diets. The 25(OH)D was analyzed by the chemiluminescence method. Serum levels of 25(OH)D are not associated with sex, but the concentration is lower in puppies (30.6 ng/mL) than in adults (34.9 ng/mL) (G1),P
Source: Comparative Clinical Pathology - May 18, 2020 Category: Pathology Source Type: research

Purple onion in combination with garlic exerts better ameliorative effects on selected biomarkers in high-sucrose diet-fed fruit fly ( Drosophila melanogaster )
AbstractGarlic and purple onion are two vegetables widely used globally for culinary purposes. These vegetables have been previously reported to exhibit several medicinal properties including antidiabetic, antihypertensive, and renoprotective properties owing to their constituent bioactive phytochemicals. However, both vegetables are often combined in popular culinary practices and there is a dearth of information on their combinatorial effects. Therefore, this study evaluated the effect of garlic, onion, and their combination on high-sucrose diet (HSD)-Drosophila melanogaster model. HSD-drosophila is gaining popularity as...
Source: Comparative Clinical Pathology - May 18, 2020 Category: Pathology Source Type: research

Osteosarcoma in a Dachshund: cytological and histopathological aspects
This report alerts clinicians to the occurrence of osteosarcoma in small animals and demonstrates the importance of fine-needle aspiration cytology as a tool in the diagnosis of bone neoplasms. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 17, 2020 Category: Pathology Source Type: research

Clinicopathological features, molecular diagnosis and treatment of epizootic lymphangitis in a donkey at a university zoological garden: a case report
AbstractReport on epizootic lymphangitis, a chronic contagious zoonosis ofEquidae caused byHistoplasma capsulatum var.farciminosum characterized clinically by pyogranulomatous multifocal dermatitis with lymphadenitis, is rare in donkeys. This case reports a 6-year-old, 200-kg local male donkey at the University of Ibadan zoological garden with wounds on its dorsum which developed into ulcerative lesions on the left cranial thoracic region. The lesions were also along the lymphatic drainage of the left infrascapular area down to the girth with extensive nodular lesions on the skin, characteristic focal ulcers and fenestrati...
Source: Comparative Clinical Pathology - May 16, 2020 Category: Pathology Source Type: research

Pathological findings in a fatal pet rabbit Pasteurellosis
AbstractA case of fatal Pasteurellosis infection in a 2-month-old rabbit is described. Pasteurellosis is considered one of the most important diseases of rabbits and has been reported as a constant serious and highly contagious disease of domestic rabbits. Before death, the main clinical signs observed were rhinitis with nasal discharge, abdominal breathing and coughing. At necropsy, pathological findings revealed severe pleuropneumonia with the accumulation of a remarkable amount of fibrinopurulent exudate in the thoracic cavity, serous rhinitis and tracheitis. A bacterial isolate from lungs was identified asPasteurella m...
Source: Comparative Clinical Pathology - May 16, 2020 Category: Pathology Source Type: research

Note on the Mesopotamian spiny-tailed lizard, Saara loricata (Blanford, 1874): morphometrics and evidence for gender partitioning of hematological data
The objectives of this study were to document morphometrical measurements and hematological data of clinically healthy free-ranging adult Mesopotamian spiny-tailed lizards (Saara loricata), the Iraqi Mastigure or Iraqi spiny-tailed lizard, from Khuzestan Province, Iran, and to investigate sex differences in evaluated parameters. Although we did not observe any gender differences in morphometrical measurements, the visually larger genital pores of males allowed for differentiation of study animals into females (n = 6) and males (n = 5). We obtained femoral vein blood samples for hematological ana...
Source: Comparative Clinical Pathology - May 7, 2020 Category: Pathology Source Type: research

Assessment of biochemical and immunohistochemical changes in buffalo-cows with reproductive disorders
In this study, 25 buffalo-cows (normal cyclic,n = 9, pluriparous; anestrus,n = 7, nulliparous and repeat breeders,n = 9, pluriparous) were used to assess some biochemical and immunohistochemical changes in those that had reproductive disorders in comparison with normal cyclic animals. Serum calcium (Ca) and vitamin D (25(OH)D) levels were analyzed. Also, serum progesterone (P4) and estradiol (E2) levels were measured to assess the animals’ reproductive statuses. Additionally, immunohistochemical expression of calcium-binding protein-D9k (CaPB-9k) and vitamin D receptor (VDR) ...
Source: Comparative Clinical Pathology - April 30, 2020 Category: Pathology Source Type: research

Complete blood count evaluation of dogs treated with four different antineoplastic chemotherapy protocols
AbstractThis retrospective study aimed to evaluate potential hematological alterations in dogs treated with four different antineoplastic protocols. We selected 69 dogs that received at least four chemotherapy sessions and divided them into four groups: vinblastine (VBL) (n = 30), doxorubicin (DOX) (n = 15), combined CHOP protocol (cyclophosphamide, doxorubicin, vincristine, and prednisone) (n = 11), and vincristine group (VCR) (n = 13). Hematological parameters were recorded from blood samples collected in five periods: T0 (before the start of treatment), T1, T2, T3, and T4 ...
Source: Comparative Clinical Pathology - April 24, 2020 Category: Pathology Source Type: research

Effects of pirfenidone, vitamin E, and pirfenidone –vitamin E combination in paraquat-induced pulmonary fibrosis
AbstractParaquat-induced pulmonary fibrosis is a progressive and fatal interstitial lung disease, a condition for that, there is no effective treatment and its prognosis is appalling. Since multiple coactivated pathways are involved, targeting these pathways using combination regimens is plausible for successful therapy. So, the aim of the present study was to evaluate the therapeutic efficacy of pirfenidone –vitamin E combination therapy in (paraquat) PQ-induced lung fibrosis model. After the development of PQ-induced lung fibrosis, pirfenidone, vitamin E, pirfenidone plus vitamin E, and water (as a vehicle) were or...
Source: Comparative Clinical Pathology - April 24, 2020 Category: Pathology Source Type: research

Comparative effect of quail and chicken egg consumption on lipid profile, total proteins and blood parameters of male albino rats ( Rattus norvegicus )
This study evaluated the comparative effect of feeding quail egg or chicken egg on the lipid profile, total proteins and blood parameters of male albino rats. Male albino rats (36) weighing between 100 and 120  g were used for this study. They were randomly assigned into 7 groups of 5 rats each. Groups A1, A2 and A3 were dosed with quail egg at 0.25 mg/kg, 0.5 mg/kg and 0.75 mg/kg, respectively. Groups B1, B2 and B3 received 0.25 mg/kg, 0.5 mg/kg and 0.75 mg/kg, respectively, of chicken egg. The co ntrol group received distilled water. Blood for haematology, lipid profile and total protei...
Source: Comparative Clinical Pathology - April 19, 2020 Category: Pathology Source Type: research

Parkinsonia aculeata L. aqueous extract alleviated the hepatotoxicity induced by acetaminophen in albino rats
AbstractHepatotoxicity is considered a serious condition which can be progressed to liver damage. The hepatoprotective effect ofParkinsonia aculeata (PA) aqueous extract against acetaminophen (APAP)-induced hepatotoxicity was investigated. Wister albino rats were orally pre-treated with PA aqueous extract (250  mg/kg BW) for 14 days. A single toxic dose of APAP (800 mg/kg BW) was orally administered at the 13th day. Collection of blood samples was done for the biochemical analysis, and the hepatic tissue was collected for histopathological examinations. Our results indicate that PA aqueous extract exhi bit a...
Source: Comparative Clinical Pathology - April 16, 2020 Category: Pathology Source Type: research

Pathological and molecular detection of a rare case of cutaneous cryptococcosis in a 9-year-old horse
This report describes a rare case of primary cutaneous cryptococcosis in equines. A young gelding horse was presented with facial masses showing ulcerations and yellowish discharge. Histopathological examination of biopsy specimens showed a dermal granulomatous reaction containing neutrophils, macrophages, and giant cells. In addition, numerous spherical yeast-like organisms were clearly observed within the granulomatous reaction. We suspected that the observed organisms belong to theCryptococcus spp., which was indeed by its shape on Sabouraud dextrose agar, Gram-stained smear suggestiveC. neoformans, and confirmed on PCR...
Source: Comparative Clinical Pathology - April 14, 2020 Category: Pathology Source Type: research

The effects of selenium nanoparticles (SeNPs) on oxidant and antioxidant activities and neonatal lamb weight gain pattern
AbstractSelenium (Se) is an antioxidant element that prevents the oxidative stress. Se incorporates into proteins such as selenocysteine and prevents oxidative damage to body tissues. In neonatal period, the different types of stress such as hypoglycemia and hypothermia may predispose them to many types of infectious diseases and also reduce growth rate at postnatal period. For these reasons, the present study was conducted on 12 newborn lambs to evaluate the effects of oral selenium nanoparticle (SeNP) supplementation on serum levels of selenium, copper, zinc, thiobarbituric acid reactive substances (TBARS), superoxide di...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Glucose levels are stable in stored blood and plasma samples of cultured female African catfish ( Clarias gariepinus )
AbstractGlucose is utilized by fish as one of the sources of energy. It is a physiological parameter useful in the determination of the general health condition of cultured fish. The aim of this study was to determine the stability of plasma and blood glucose concentration, stored at different temperatures (4 °C and 30 °C) for 72 h. Two (2) milliliters of blood was collected via the caudal abdominal venipuncture of 10 female catfishes purchased. The fish were 8 months of age and were kept in plastic ponds at the Faculty of Veterinary Medicine, University of Nigeria. Blood was collected in blood tubes c ontaining K2...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Short-term effects of feeding powdered Aloe vera ( Aloe barbadensis ) and nettle ( Urtica dioica ) on growth performance and stimulation of innate immune responses in rainbow trout ( Oncorhynchus mykiss )
AbstractTo evaluate the nutritional impacts of powdered Aloe vera and nettle plants on growth performance and some parameters of immune response in rainbow trout, a total of 462 fish with an average weight of 10.80  ± 0.59 g were obtained from authentic fish farms. The fish were fed in seven groups with diets containing 0.5, 1, and 1.5% of Aloe vera powder and 0.5, 1, and 1.5% of nettle powder, and also a control with a basal diet without herbal supplementation for 4 weeks. The results of fish biometry showed that dietary supplementation with the powdered plants led to significant differences...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Effect of high-intensity interval training on expression of microRNA-149 and genes regulating mitochondrial biogenesis in doxorubicin-cardiotoxicity in rats
AbstractWe have investigated the effect of high-intensity interval exercise (HIIT) on DOX-induced cardiotoxicity and the expression of mitochondrial biogenesis genes SIRT-1, PGC-1 α, and NRF-2 as well as microRNA-149-5p in a rat model. Twenty-four male Wistar rats (250–270 g) were randomly divided in four groups (n = 6/each): control, HIIT, DOX, and HIIT+DOX. HIIT was performed on a treadmill as seven alternative intervals of high and low intensity trainings for 1 h a day for 6 weeks. After the last session of HIIT, DOX was injected i.p. to trained and time-matched control rats. Aft...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Biochemical and total hemocyte count profiles of long-clawed crayfish ( Astacus leptodactylus ) as a reference interval in southern Iran
This study was aimed to determine the normal interval of the biochemical parameters in these species in Iran, which appears to be necessary for future plannin g and studies, especially for ecotoxicological studies. Biochemical parameters analyzed in this study were glucose (11.37 ± 1.53 mg/dl), urea (5.58 ± 0.62 mg/dl), creatinine (0.21 ± 0.02 mg/dl), AST (7.54 ± 0.82 U/L), ALT (7.04 ± 0.97 U/L), ALP (380.44 ± 33.46 U/L), total bilirubin (0.45 ± 0. 32 mg/dl), calcium (8.39 ± 1.15 mg/dl), phosphorus (2.35 ± 0.24 mg/dl), total protein (1.72 ± 0.16 g/dl), a...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Murine leukocytes stimulation by Toxoplasma gondii antigens induces high level of IFN- γ production
AbstractToxoplasma gondii (T.gondii) is an obligate intracellular parasitic protozoan, infecting a wide range of warm-blooded vertebrates. It is important forT. gondii to balance the response between cellular and humoral immune system for various aspects of its own survival. The aim of this study was to determine the level of IFN- γ and IL-4 production by murine peritoneal leukocytes stimulated withT. gondii lysate antigens (TLAs), excretory/secretory antigens (ESAs), and a combination of them (TLAs:ESAs). TLAs, ESAs, and TLA  + ESA, considered as the experimental groups, were prepared and used to stimu...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Neurobehavioural and repeated-dose toxicity studies on the extractive from the decoction of a mixture of Alstonia boonei and Picralima nitida in mice
This study evaluated the central nervous system effects and the toxicity of the remedy on acute and repeated doses with a view to providing information on its safety. The powder mixture (ratio 1:2) ofA. boonei stem-bark andP. nitida seed was extracted with water by the decoction method, concentratedin vacuo and freeze-dried. The acute toxicity of the extractive was determined by Lorke ’s method. For the novelty-induced behaviour tests, 24 mice (18–22 g) of four groups (six mice per group) were orally given the aqueous solution of the extractive at 12.5, 25 and 50 mg/kg while the control group was given distille...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Circulatory levels of pro-inflammatory cytokines (IL-6 and IL-1 β) and neutrophil-lymphocyte ratio (NLR) in diabetic patients in Nigerian population
This study was designed to evaluate pro-inflammatory cytokine levels (IL-6 and IL-1 β) and NLR in T2DM subjects in Nigerian population. This was a case-control study involving 75 physician diagnosed T2DM subjects attending the Endocrine Clinic of a Tertiary Health Institution in Nigeria and 75 age- and sex-matched healthy individuals (used as control) who were recruited from the B lood Donor Center of the same institution. Based on physician assessment, the diabetic subjects were grouped differently into those with complications (48) and those without complications (27). Serum levels of IL-6 and IL-1β were measur...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Anti-inflammatory activities of crude ethanol extract of Combretum zenkeri Engl. & amp; Diels leaves
ConclusionThis study shows that EECZ possess anti-inflammatory activities that could be through the inhibition of PLA2 activity and platelet aggregation, and membrane stabilization. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Effect of cannabinoid-serotonin interactions in the regulation of neuropeptide Y1 receptors expression in rats: the role of CB1 and 5-HT 2C receptor
AbstractNeuropeptide Y (NPY) is involved in a diversity of critical functions such as circadian rhythms, energy homeostasis, and appetite regulation in the hypothalamus. It has identified as a crucial participant in adjusting energy intake and energy storage as fat via central neuropeptide Y1 receptor (NPY1R), leading to obesity and metabolic disorders. The present study was expected to investigate the interaction between 2-AG (CB1R agonist), m-CPP (5HT2CR agonist), SB-242084 (5HT2CR antagonist), and SR-141716A (CB1R antagonist) by mediating through the NPY1R for treating or preventing obesity, metabolic disorders, and oth...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

First report of pedunculate barnacle ( Octolasmis neptuni ), as potential asymptomatic carrier of white spot syndrome virus (WSSV)
AbstractWhite spot syndrome virus (WSSV) is a notorious virus infecting cultured shrimps affecting the aquaculture industry for more than two decades over the world. WSSV has been isolated from asymptomatic carriers including crabs, artemia, rotifers, polychaetes and insects. Asymptomatic carriers are hosts that do not exhibit infection symptoms but carries infection to other hosts. The potential virulence of asymptomatic carrier of WSSV in multiple hosts were screened in the current study. The symbioticOctolasmis neptuni isolated from the gill lamellae of moribund mud crab (Scylla olivacea) is suspected to cause WSSV infe...
Source: Comparative Clinical Pathology - March 6, 2020 Category: Pathology Source Type: research

Neuroprotective effects of gallic acid against neurotoxicity induced by sodium arsenite in rats
AbstractArsenic causes a wide range of neurological complications including cognitive impairment, short-term memory and concentration problems. The present study investigated effects of gallic acid (GA) against sodium arsenite (SA)-induced neurotoxicity in rats. Thirty-five adult male rats were randomly divided into five groups. Group 1 received normal saline (2  ml/kg, P.O.) for 21 days. Group 2 received SA (10 mg/kg, P.O., for 14 days). Groups 3 and 4 received GA (10 and 30 mg/kg, P.O., respectively) for 7 consecutive days prior to SA treatment and continued up to 21 days, parallel to SA adm...
Source: Comparative Clinical Pathology - March 2, 2020 Category: Pathology Source Type: research

Methanol extract of Napoleona vogelii demonstrates anti-nociceptive and anti-inflammatory activities through dopaminergic mechanism and inhibition of inflammatory mediators in rodents
This study was conducted to investigate the anti-nociceptive and anti-inflammatory activity of the methanol stem bark extract ofN. vogelii in rodents. The extract (100 –400 mg/kg) was administered 1 h before intraperitoneal and intraplantar injection of 0.2 mL of 0.6% v/v acetic acid and 0.05 mL of 2% v/v formalin, respectively. Anti-inflammatory activity was investigated using the carrageenan-induced paw edema and formalin-induced arthritis models. Mechanis tic studies were conducted by exploring opioidergic, dopaminergic pathways, and inflammatory mediators. There was 62.26% inhibition of inflam...
Source: Comparative Clinical Pathology - February 28, 2020 Category: Pathology Source Type: research

Correction to: Anticipation of estrus by the use of bromocriptine (Parlodel 2.5 mg) in Atlas Shepherd bitch: cytological and echographic follow-up
The author of this article would like to add another affiliation, which is reflected here. This correction does not, in any way modify or change the scientific validity of the results presented in this manuscript. The author sincerely apologizes for this error. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 27, 2020 Category: Pathology Source Type: research

Vitamin D dietary supplementation ameliorates the complications associated with type 2 diabetes induced by streptozotocin
AbstractType 2 diabetes is a major public health problem, accounting for significant premature mortality and morbidity. It has been reported to be associated with several chronic complications which involve coronary artery disease and renal and liver diseases that are considered the primary cause of disability and mortality in diabetic patients. Different studies stated that vitamin D have functional role in glucose tolerance through its effects on insulin secretion and sensitivity and ameliorate type 2 diabetes complication. For that this study was carried out to investigate the effect of dietary vitamin D on type 2 diabe...
Source: Comparative Clinical Pathology - February 13, 2020 Category: Pathology Source Type: research