Azadirachta indica (A. Juss.) and Curcuma longa (L.) modulate immunoglobulin and cytokine levels in Plasmodium berghei-infected mice
AbstractPlasmodium infection causes inflammation in the host. A combination of herbs likeAzadirachta indica andCurcuma longa may have beneficial effect in remediating inflammation in malaria disease. The aim of this study is to investigate the immunomodulatory and synergistic effects of the association ofAzadirachta indica andCurcuma longa in experimentalPlasmodium berghei infection. Fifty male Swiss mice were infected with chloroquine susceptible (NK 65) strain ofPlasmodium berghei intraperitoneally. Parasitemia was confirmed using microscopy and the animals were grouped (n = 5), treated once daily and orall...
Source: Comparative Clinical Pathology - September 20, 2021 Category: Pathology Source Type: research

Protective effect of betaine against gentamicin-induced renal toxicity in mice: a biochemical and histopathological study
AbstractConsidering the role of oxidative stress as a molecular mechanism underlying the deleterious side effects of gentamicin, applying antioxidants is likely to have ameliorative effects against GNT-induced tissue damages. This work was aimed to investigate the protective effect of betaine or trimethylglycine upon gentamicin-induced toxicity in mice. Thirty male mice were randomly divided into five groups: group 1 (control; i.p. injection of isotonic saline), group 2 received gentamicin (GNT; 80  mg/kg, i.p.) for 10 days, group 3 received GNT (80 mg/kg, i.p.) for 10 days and betaine (2% in the diet) ...
Source: Comparative Clinical Pathology - September 18, 2021 Category: Pathology Source Type: research

Oxidant-antioxidants and trace mineral status in Coccidiosis affecting buffalo calves
This study aimed to investigate the biochemical, and oxidative stress parameters in diarrhetic buffalo calves. A total number of 16 buffalo calves enrolled in this study. Calves aged between 3 and 6  months and divided into diarrhetic calves (N = 10) and healthy calves (n = 6). Clinical signs were recorded, and fecal samples were taken and examined for the presence ofEimeria oocytes. A total of 10  ml of blood was withdrawn from the jugular vein to estimate superoxide dismutase (SOD) and glutathione peroxidase (GPx), catalase (CAT), malondialdehyde (MDA), total antioxidant capacity (TA...
Source: Comparative Clinical Pathology - September 17, 2021 Category: Pathology Source Type: research

Revealed pathophysiological mechanisms of crosslinking interaction of affected vital organs in COVID-19
AbstractSARS-CoV-2 is one of the main serious challenges of human societies, which emerged in December 2019 from China and quickly extends to all parts of the world. The virus was previously believed to only affect the lungs and respiratory system, but subsequent research has revealed that it affects a variety of organs. For this reason, this disease is known as a multiorgan disease. Current article aimed to highlight latest information and updates about molecular studies regarding pathogenesis of SARS-CoV-2 in kidney, liver, and cardiovascular and respiratory systems, as well as the mechanisms of interaction of these orga...
Source: Comparative Clinical Pathology - September 10, 2021 Category: Pathology Source Type: research

Effects of graded levels of Adansonia digitata leaves on semen quality in Red Sokoto bucks
This study was designed to determine the effects of graded levels ofAdansonia digitata leaves on semen quality in Red Sokoto bucks (RSB). Twenty RSB were randomly assigned into four groups comprising 5 RSB each. Groups A, B, C, and D were given 0%, 10%, 20%, and 30% ofA. digitata leave powder, respectively, for 14  weeks. Semen was collected every 2 weeks and evaluated for semen volume, sperm cell motility, semen pH, percentage sperm abnormalities, sperm livability, semen concentration, colour, and consistency. There were no significant (p >  0.05) differences in semen volume, pH, and sperm livab...
Source: Comparative Clinical Pathology - September 4, 2021 Category: Pathology Source Type: research

Evaluation of therapeutic effect of Psidium guajava root extract against the toxicity induced by Ricinus communis (castor oil) in rats
AbstractThe present study evaluated the therapeutic potential of root extracts ofPsidium guajava—a medicinal plant used by the indigenous people of Nigeria to treat hepatitis and other health conditions. Thirty male rats were divided into six groups of five rats. Group 1 received 20 mL/kg body weight of distilled water (normal control). Group 2 was induced with 100 mg/kg body weight of cas tor oil (positive control); group 3 was induced with 100 mg/kg of castor oil and treated with silymarin (standard control). Groups 4, 5, and 6 were induced with castor oil each and treated with 200, 400, and 600 ...
Source: Comparative Clinical Pathology - September 3, 2021 Category: Pathology Source Type: research

Protective effects of hydro-ethanolic leaf extract based formulation of Mucuna pruriens (Fabaceae) on phenylhydrazine induced hemolytic anemia and metabo-hematological alterations in rats
This study is aimed at evaluating the use ofMucuna pruriens herb in the treatment of hemolytic anemia secondary to malaria. Thirty-six adult male rats weighing between 150 and 200  g were divided into six groups (n = 6). Group I served as the negative control and received syrup formulation without the extract (placebo) but allowed access to food and water ad libitum. Groups II to VI received phenyl hydrazine 60 mg/kg for 3 days to induce anemia. Additionally, groups IV to VI received 50 mg/kg, 100 mg /kg, and 200 mg/kg ofMucuna pruriens syrup formulation respectively, while ...
Source: Comparative Clinical Pathology - September 2, 2021 Category: Pathology Source Type: research

Clinical report of Coenurosis cerebralis outbreak in Lori sheep
AbstractCoenurosis is a parasitic disease in livestock animals caused byTaenia multiceps, whose adult parasite lives in the intestine of the Canidae and ruminants and the most important symptoms are nervous system involvement. Five Lori sheep from a flock with neurological symptoms, seizures, and fatalities were referred to the Veterinary Teaching Hospital of Lorestan University with a history of no antiparasitic treatment for the past 6 months. Clinical examination showed no significant changes in vital signs and further hematobiochemical investigation revealed eosinophilia and hyperproteinemia in affected sheep. For furt...
Source: Comparative Clinical Pathology - August 31, 2021 Category: Pathology Source Type: research

Blood parameters of the Eastern Pacific green turtle (Chelonia mydas) foraging in the Golfo Dulce, Costa Rica
In this study, values for hematology and biochemistry of 85 green turtles captured from 2010 to 2014 were analyzed. These animals correspond to a resident foraging population at Golfo Dulce, Costa Rica. All of them underwent a physical examination and were deemed apparently healthy. Hematological analyses were done using manual methods, while seric metabolites were determined with automated and semi-automated spectrophotometry. According to the curved carapace length (CCL) measurements, the subadult individuals were the most frequent (n = 74). The predominant cells were heterophils, followed by lymphocytes. B...
Source: Comparative Clinical Pathology - August 31, 2021 Category: Pathology Source Type: research

Histological and clinical outcome of a novel case of perineal hamartoma, atresia ani, and rectovaginal fistula in female Holestein calf
AbstractThe association of anorectal congenital malformation and perineal mass are extremely rare at veterinary literature. The present report is a novel case of perineal hamartoma, atresia ani, and rectovaginal fistula in female calf, 2-day-old female Holstein Friesian calf. The animal had a fleshy, dark red, trailing perineal mass at the left side, imperforated anus and about 1  cm rectovaginal fistula. Imperforated anus and rectovaginal fistula were surgically corrected. The mass was firm, red in color and contain bean-shaped, white color tissues on cut section. It was misdiagnosed as teratoma, but the careful hist...
Source: Comparative Clinical Pathology - August 29, 2021 Category: Pathology Source Type: research

Retrospective study of the aetiopathological diagnosis of pleural or peritoneal effusion exams of dogs and cats
AbstractThe cavitary fluids exams assist in the diagnosis of diseases in veterinary medicine, but sometimes, this exam is not much requested. The aim of this study was to identify what diagnoses are most recurrent in relation to pleural and abdominal effusions laboratory findings, as well as the relationships of canine and cat gender and age. The laboratory and clinical data from 416 cases of canine cavitary effusions from 2008 to 2015 were retrospectively assessed from the University Veterinary Hospital, Santa Maria, RS, Brazil. Out of the 419 samples included in this study, 311 samples were from dogs and 105 from cats. T...
Source: Comparative Clinical Pathology - August 27, 2021 Category: Pathology Source Type: research

Detection of monoclonal or oligoclonal integration of bovine leukemia virus proviral DNA by inverse polymerase chain reaction for diagnosis of enzootic bovine leucosis
This study evaluated the use of inverse polymerase chain reaction (PCR) as a diagnostic method for EBL. Inverse PCR revealed monoclonal or biclonal integration of BLV proviral DNA in the genomes of 71 of 82 cattle with EBL, and polyclonal integration of BLV proviral DNA in the genomes o f 16 cattle with B-cell type sporadic bovine leukosis (SBL), 5 cattle with T-cell type SBL, 4 cattle with tumors other than lymphoma, 20 cattle with inflammatory diseases, 5 cattle with diseases other than tumors and inflammatory diseases, and 20 healthy cattle. The sensitivity, specificity, positiv e predictive value, and negative predicti...
Source: Comparative Clinical Pathology - August 18, 2021 Category: Pathology Source Type: research

Pathological and molecular diagnosis of concurrent canine babesiosis and pyometra in a Belgian Malandis bitch
AbstractVarious authors have performed microscopic and molecular diagnosis and pathological evaluations of babesiosis as well as the clinical, bacteriological, and uterine histopathological investigations  of pyometra separately in dogs. However, the present report investigated a case of concurrent canine babesiosis and pyometra in a dog with particular emphasis on histopathological evaluations associated with multi-organ failure and death arising from both conditions. The collection of appropriate samples for ante-mortem hematological and microbial evaluations, including post-mortem molecular diagnosis and histo...
Source: Comparative Clinical Pathology - August 13, 2021 Category: Pathology Source Type: research

Cardiac remodeling, apoptosis-related process (Bax, Bcl-2), and their ratio (Bax/Bcl-2) in cardiomyocytes of diabetic rats after combined exercise training and taurine supplementation
This study evaluated the effects of Taurine supplementation plus endurance and resistance training program on cardiomyocyte apoptosis and  myocardial fibrosis in the diabetic rat model. Male Wistar rats were assigned randomly into five groups of eight animals: control group, diabetes mellitus (DM) group, DM + Taurine supplementation group, DM + combined exercise training (endurance and resistance exercise training, ERE), and DM + a combinati on of Taurine and ERE. Rats were first treated with an intraperitoneal injection (i.p) of nicotinamide (100 mg/kg body weight) ...
Source: Comparative Clinical Pathology - August 11, 2021 Category: Pathology Source Type: research

Protective effect of selenium in prednisolone testicular toxicity
AbstractThe most common side effects of corticosteroids are adrenal suppression and the subsequent complications that can be attributed to testicular tissue degradation and dysfunction. Selenium or sodium selenite concentration indicates the protective role of this rare element and its related enzymes during spermatogenesis. The concentration of selenium in testis is regulated by a homeostatic mechanism that precedes the capacity of selenium in male gonads from other tissues. Therefore, the aim of this research was to investigate the protective effects of selenium on tissue structure and performance of testis in mice. In t...
Source: Comparative Clinical Pathology - August 10, 2021 Category: Pathology Source Type: research

Disease and mortalities in selected zoological gardens in Nigeria
This study enumerates the common diseases, species affected, in wild captive animals in three zoological gardens in Nigeria, making the ation available to clinicians, biologists, pathologists, public health workers, and policy makers. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - August 10, 2021 Category: Pathology Source Type: research

The effects of single intramuscular injection of vitamin D3 on minerals, hormone, and bone markers responses of multiparous Holstein cows fed a diet with negative dietary cation anion difference
This study showed that a single IM injection of Vit. D3 pre-partum in dairy cows resulted in lower PTH, lower CrL, and decreased ICa post-partum. There was no benefit of a single intramuscular injection of vitamin D3 in close-up cows fed negative DCAD diets on Ca metabolism. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - August 10, 2021 Category: Pathology Source Type: research

Surgical, histological, and immunohistochemical approach to spontaneous malignant mammary tumor in a fancy rat (Rattus norvegicus domestica): a case report
AbstractA domestic rat (Rattus norvegicus domestica) was presented to the Department of Veterinary Medicine of the University of Bari “Aldo Moro” (Bari, Italy) for the presence of a progressive growing unilateral mass. The mass was completely surgically removed, and then histologically and immohistochemically evaluated. The lesion was diagnosed as mammary ductal carcinoma based on histopathological examination. Immunohistochem istry staining for estrogen receptor alpha (ERα) and proliferation marker Ki-67 were found to be positive. The rat was rechecked 3 months thereafter, with total body radio...
Source: Comparative Clinical Pathology - August 7, 2021 Category: Pathology Source Type: research

Ameliorative potential of resveratrol on live body weight, glycaemic profile and serum liver enzymes in cholesterol diet-fed rabbits
AbstractAccumulation of fat in the body has been linked with an increased body mass and subsequently blood sugar level, which enhances hepatocellular damage. Increased in activities of serum liver enzymes in the above conditions has been extensively used as a mark of liver disease and predicted development of type 2 diabetes. The main objective of this research work was to investigate the effect of resveratrol on body weight, blood glucose level, and liver enzymes in rabbits. Rabbits were distributed into six groups of five animals each: Group 1 = negative control (NC), Group 2 = cholesterol diet (ChD/CD) alone, Group 3 = ...
Source: Comparative Clinical Pathology - August 7, 2021 Category: Pathology Source Type: research

Molecular investigation and phylogenetic analysis of Jaagsiekte sheep retrovirus in naturally ovine pulmonary adenocarcinoma infected small ruminants in Andhra Pradesh, India
AbstractOvine pulmonary adenocarcinoma (OPA) is a neoplastic contagious chronic disease infecting sheep and goats.Jaagsiekte sheep retrovirus (JSRV) is an aetiological agent causing OPA in small ruminants. JSRV was first isolated and whole genome sequenced in South Africa. The OPA clinical manifestation shows overproduction of lung fluid, dyspnoea and pyrexia with cough resembling pneumonia. Pathologically infected lung tissue shows greyish white, moist, enlarged, heavy and oedematous characteristics. The total RNA from OPA infected sheep and goat samples were isolated by Trizol method and converted to cDNA using poly dTP ...
Source: Comparative Clinical Pathology - August 7, 2021 Category: Pathology Source Type: research

The efficiency of Cressa cretica (Linn.) extract in attenuating vancomycin-induced nephrotoxicity and oxidative stress in rabbits
AbstractThe present study was aimed to assess the nephroprotective effect ofCressa cretica hydroalcoholic extract (CCE) on VAN-triggered renal toxicity in rabbits. Total phenolic and flavonoid contents found in CCE were 30.92  ± 2.93 mg GAE/g extract and 11.87 ± 1.70 mg CE/g extract, respectively. The CCE demonstrated a significant 2,2-diphenyl-1-picrylhydrazyl radical (DPPH*) scavenging ability with an IC50 value of 24.99  µg/mL. To evaluate the nephroprotective potential of CCE, thirty-six rabbits were divided into six groups (n = 6) and treated...
Source: Comparative Clinical Pathology - August 6, 2021 Category: Pathology Source Type: research

Comparison of fluorescent immunoassay (FIA) and electrochemiluminescence immunoassay (ECLIA) for the measurement of serum TSH in congenital hypothyroid screening
This study aimed to compare the serum thyroid-stimulating hormone (TSH) measurement using fluorescent immunoassay (FIA) and electrochemiluminescence immunoassay (ECLIA) for CH screening. CH is not yet regularly screened since the gold standard methods for measuring TSH in newborns (i.e., enzyme-linked immunosorbent assay (ELISA) and fluorescence enzyme  immunoassay (FEIA)) are high-cost or difficult to perform in remote areas. A cross-sectional study was conducted. Serum samples were consecutively taken from newborns aged 2–7 days in January–April 2018. Serum TSH was measured simultaneously by FIA (FR...
Source: Comparative Clinical Pathology - August 5, 2021 Category: Pathology Source Type: research

Application of serum and urinary electrophoresis as an aid tool for evaluating dogs at different stages of chronic kidney disease
AbstractChronic kidney disease (CKD) is a common, progressive, and irreversible disease in dogs. Inflammation and proteinuria are related to the process of CKD progression. The search for early markers and indicators of the degree and location of renal inflammation to aid in diagnosis and prognosis is very important. The aim of this study was to evaluate the applicability of serum and urinary electrophoresis as an aid tool in the diagnosis of dogs with chronic kidney disease according to the stage of CKD (IRIS2019b). For this, 27 canine patients from routine clinical practice were evaluated and divided into five groups: C ...
Source: Comparative Clinical Pathology - July 31, 2021 Category: Pathology Source Type: research

Prevalence, intensity, and impacts of non-cutaneous lesions of lumpy skin disease among some infected cattle flocks in Nile Delta governorates, Egypt
This study was designed to focus light on non-cutaneous lesions (prevalence, intensity, and impacts) among necropsied LSD infected cattle. We selected some dairy and beef flocks (Frisian breed) located in 3 governorates (Sharkia, Dakahlia, and Kaloubia) in Nile delta, Egypt, in the period from January 2019 to January 2020 for our survey study. The case history of farms declared no previous vaccination of examined farms. The clinical signs, morbidity, and mortality rates were recorded. Average morbidity and mortality percentage were 22.28% and 6.59%, respectively. PCR for specimens from liver, kidneys, heart, lungs, testis,...
Source: Comparative Clinical Pathology - July 24, 2021 Category: Pathology Source Type: research

Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma: a meta-analysis
This study aimed to evaluate the prognostic value of NLR among LSCC patients and the association between NLR and clinical characteristics. A systematic search was done through the PubMed, Embase, Scopus, CNKI, Wan Fang, and Web of Science databases. Hazard ratios (HRs) were extracted to estimate the link between NLR and prognosis of LSCC patients, including overall survival (OS), disease-free survival (DFS), and progression-free survival (PFS). Odds ratios (ORs) were used to evaluate the association between NLR and clinical characteristics. Fourteen studies were enrolled in the meta-analysis. The results showed that a high...
Source: Comparative Clinical Pathology - July 22, 2021 Category: Pathology Source Type: research

Comparative effects of meloxicam and dexamethasone in chickens with experimental systemic inflammation: clinical outcome and cardiovascular parameters
In conclusion, a single administration of meloxicam or dexamethasone was effective in the acute management of a systemic inflammatory process in chickens. Considering both effects and plausible side effects, dexamethas one at 2 mg/kg appears to be preferable in terms of therapeutic effects. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 21, 2021 Category: Pathology Source Type: research

Hematological referential interval of clinically healthy canines in the semi-arid region of Northeast Brazil
AbstractCanines are dispersed around the world, adapting physiologically to regional climatic variations, which can trigger an amplitude in the variability of referential clinical and laboratory aspects. Thus, we aim to determine the hematological reference interval for clinically healthy dogs located in the semi-arid region of Northeast Brazil. The retrospective study was carried out between February and June 2018 with complete blood count data from 126 healthy dogs with no predilection for sex, breed, and age, selected on the basis of visits for elective castration and ovariohysterectomy. The data set for defining the re...
Source: Comparative Clinical Pathology - July 19, 2021 Category: Pathology Source Type: research

Effect of thyme (Thymus vulgaris) powder on acid-base balance, rumen, and blood constituents in healthy Egyptian sheep
This study was carried out to investigate the effects of thyme powder on acid-base balance, rumen, and blood constituents in apparently healthy Egyptian sheep. Ten Egyptian ewes were given thyme powder (500 mg/kg bodyweight) orally in the morning before breakfast feeding for 5 days. Blood samples and rumen juice were collected in the morning on zero (control), 3rd, and 5th day before feeding. There was a significant increase in blood pH, bicarbonate, base excess, rumen fluid pH, WBCS, lymphocytes, MCH, and MCHC; on the other hand, total protozoal count, total volatile fatty acids, and total serum protein were significantly...
Source: Comparative Clinical Pathology - June 26, 2021 Category: Pathology Source Type: research

Neuromodulatory evaluation of commonly abused plants ex vivo: a comparative study
In conclusion,D. stramonium altered critical neuronal enzymes significantly more than the illicit plant of abuse, whileNicotiana tabacum showed no significant difference in comparison toC. sativa. Therefore, the use of these plants as drugs should be discouraged. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 26, 2021 Category: Pathology Source Type: research

Higher hepatic sterol-binding protein-2 gene expression and serum triglyceride level in methimazole induced subclinical hypothyroid animal model
AbstractSubclinical hypothyroidism (SCH) is a thyroid disorder characterized with elevated TSH level. Recent investigations indicate association of SCH with cardiovascular complications and dyslipidemia. Here, we aimed to evaluate the dyslipidemia in rat model of SCH induced with methimazole (MM) administration. For SCH induction, MM was administrated using gavage or ad libitum in drinking water to rats. Serum TSH and T4 levels were measured using ELISA method. The cholesterol and triglyceride were measured in serum samples using colorimetric assays. Lipid accumulation in the liver tissue was assessed histologically using ...
Source: Comparative Clinical Pathology - June 24, 2021 Category: Pathology Source Type: research

Role of enzymatic and non-enzymatic antioxidants among Egyptian neonatal sepsis
AbstractNeonatal sepsis is among the major causes of neonatal death. Oxidative stress is involved in detrimental pathways activated during neonatal sepsis, eventually leading to organ dysfunction and death. Reaching a definitive early diagnosis of neonatal sepsis is often challenging, as causative microbes typically require several days of blood culturing. The aim of this study was to evaluate the role of enzymatic and non-enzymatic antioxidants as early predictive biomarkers of neonatal sepsis and as predictors of disease outcome. For the purposes of this study, 100 neonates were recruited from the Neonatal Intensive Care...
Source: Comparative Clinical Pathology - June 19, 2021 Category: Pathology Source Type: research

Cytoprotective effect of ginger extract on cisplatin-induced hepatorenal toxicity in rats via modulation of oxidative stress, inflammation and apoptosis: histopathological, biochemical and immunohistochemical study
In conclusion, ginger extract has protective effects against CP adverse effects, possibly via its antioxidant, anti-inflammatory, and anti-apoptotic properties. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 18, 2021 Category: Pathology Source Type: research

Haematological profiles and erythrocyte fragility of Arabian stallions subjected to a 2000-m maximal race following administration of ergothioneine
AbstractExercise is a beneficial practice to the body and can constitute a challenge when taken to the extreme especially in competitive sports. Haematological parameters play a very important role in exercise and are usually good indicators of the effects of exercise on the body. In this experiment, ergothioneine was administered to stallions in the experimental group (Ex  + ERG) before exercise to evaluate its ameliorative effect on haematological profile and erythrocytes osmotic fragility against the stress of exercise. The leucocyte count post-exercise in Ex + ERG (5.33 ±&thins...
Source: Comparative Clinical Pathology - June 15, 2021 Category: Pathology Source Type: research

Reticular diaphragmatic hernia in water buffalo: clinical characteristics, haematology, biochemical analytes, and prognostic indicators
AbstractThis prospective study was conducted on 42 buffaloes diagnosed with reticular diaphragmatic hernia (RDH). The common clinical findings were depressed demeanor, chronic inappetance or anorexia, dehydration, recurrent or persistent tympany, scanty faeces, mushy rumen with normal or increased motility, tachypnea, abdominal distension, and gradual reduction in milk yield. The presence of reticular foreign bodies seemed to be the major predisposing factor. Neutrophil and lymphocyte counts were significantly (p 
Source: Comparative Clinical Pathology - June 10, 2021 Category: Pathology Source Type: research

Abrogation of cisplatin-induced nephrotoxicity in rats and HEK-293 cell lines by formononetin: in vivo and in vitro study
AbstractThe present study was performed to investigate the underlying defensive mechanisms of formononetin in nephrotoxicity by using in vivo and in vitro models. MTT assay was carried out to test the toxic effect of formononetin in HEK-293 cells which were treated with formononetin (5, 10, 20  µM) and cisplatin (20 µM). The concentrations of TNF-α and nitric oxide (NO) were determined in the cell supernatant. Nephrotoxicity in rats was induced by a single intraperitoneal injection of cisplatin at a dose of 5 mg/kg, and formononetin at doses of 10 and 30 mg/kg was used to study pro tec...
Source: Comparative Clinical Pathology - June 7, 2021 Category: Pathology Source Type: research

Comparison of seven circulating microRNAs and ALT in carbon tetrachloride –induced liver injury model in Macaca fascicularis
This study was initiated to determine the potential of circulating miR-122, miR-192, miR-885, miR-151, miR-378, miR-365, and miR-31 as a biomarker for carbon tetrachloride (CCl4) –induced liver injury inMacaca fascicularis. The tenMacaca fascicularis were orally given 15% CCl4 (10 mL/kg) to establish the DILI. The serum at 48 h after administration when a single hepatocyte showed histopathological necrosis and vacuolation was isolated and quantified for serum miRNAs by next-generation sequencing (NGS) analysis. It was revealed that miR-122, miR-192, miR-885, miR-151, miR-378, miR-365, miR-31, and miR-802 were upregul...
Source: Comparative Clinical Pathology - June 3, 2021 Category: Pathology Source Type: research

Effect of fennel essential oil and flaxseed oil on blood parameters, insulin resistance, and histological structure of ovaries in rats suffered polycystic ovary syndrome
AbstractPolycystic ovary syndrome (PCOS) is known as a reproductive disorder in mammalian females. Medical plants are known from ancient times and herbal therapy is common in many countries for preventing and treating different diseases or disorders. The effect of fennel essential oil and/or flaxseed oil on body weight, blood parameters, and histological structure of ovaries and pancreas in rat suffered PCOS, was investigated. Thirty adult Wistar female rats (BW=260 ±20 g) that 24 of them suffered by PCOS by a single intra-muscular (IM) injection of 4 mg/kg estradiol-valerate (EV) were allocated to the treatments. F...
Source: Comparative Clinical Pathology - June 1, 2021 Category: Pathology Source Type: research

In vitro toxicological evaluation of arsenic on superoxide dismutase mediated oxidative stress in renal cells of rat and its amelioration with supplementation of Pleurotus florida lectin (PFL)
AbstractConcomitant oral supplementation of Pleurotus florida lectin (PFL) (@50 μg/ml) on arsenic (@ 5 μM) exposed renal cells was investigated to evaluate the protective value of PFL against arsenic toxicity in rats. Renal cells, exposed to arsenic(III), showed a significant inhibition of cell proliferation index (CPI) and superoxide dismutase (SOD) acid and catalase (CAT) a ctivity with an increased level of lipid peroxidation (LPO). A marginally increased level of nitric oxide (NO) was found firstly and thereafter decreased due to long-term arsenic exposure in the cells. Concurrent application of PFL in arsenic ex...
Source: Comparative Clinical Pathology - June 1, 2021 Category: Pathology Source Type: research

Fig leaves varieties reduce blood pressure in hypertensive rats through modulation of antioxidant status and activities of arginase and angiotensin-1 converting enzyme
AbstractThe medicinal application of sandpaper leaves in the management of hypertension has been reported with very little knowledge on the variety with better anti-hypertensive properties. Hence, this study seeks to compare phenolic content and the effect of some varieties of sand paper leaves (Ficus spp.), namely,Ficus exasperata (FE),Ficus asperifolia (FA),Ficus mucuso (FM) andFicus capensis (FC) on the systolic and diastolic bood pressures (SBP and DBP), angiotensin-1-converting enzyme (ACE), and arginase activities and antioxidant status in L-NAME induced hypertensive rats. The animals were divided into eleven groups ...
Source: Comparative Clinical Pathology - May 31, 2021 Category: Pathology Source Type: research

Hypospadia in a zebu calf in Mexico
AbstractDuring the general management of cattle on a ranch in southern Mexico, the incidental finding of a male calf with a malformation of the genitals was made. The andrological examination revealed a case of hypospadias based on the structures evaluated and was differentiated from a hermaphrodite. Due to the type of hypospadia (perianal hypospadia), no intervention was performed, other than applying vaseline to the skin where the urine made contact. The prognosis of the case was reserved, since this type of pathology is generally associated with malformations in vital organs (i.e., in the heart and kidneys). This is the...
Source: Comparative Clinical Pathology - May 29, 2021 Category: Pathology Source Type: research

Modulation of C5a-C5aR interactions against murine mammary cancer cell line
AbstractComplement C5a is a potent inflammatory chemoattractant and might be a beneficial therapeutic target for the induction of an effective anti-tumour response. C5a agonist and antagonist modulation have been demonstrated to either promote or inhibit tumour development in EMT6 murine mammary cancer cells in both in vitro and in vivo studies. For the in vitro studies Alamar Blue cell viability assay was used for cell viability determination, and an immunofluorescence assay was used to determine the location of C5aR expression on the EMT6 cell line. For the in-vivo experiment, female Balb/c mice were subcutaneously injec...
Source: Comparative Clinical Pathology - May 27, 2021 Category: Pathology Source Type: research

Metabolic syndrome in adult male rats induced by feeding beef tallow-enriched homemade diet with fructose-containing drinking water
This study was aimed at formulating a new high-fat diet that could induce MS. Twelve male Wistar rats were separated into two equal groups. The control group received standard rat chow with tap water while the treated group received a beef tallow-enriched high-fat diet with fructose in drinking water (HFD-FDW) for 8  weeks. The food/water intake, body weight (BW), body size parameters, blood pressure, and fasting blood glucose were estimated. Serum total cholesterol (TC) triacylglycerol (TAG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were determined. The con trol gr...
Source: Comparative Clinical Pathology - May 22, 2021 Category: Pathology Source Type: research

Establishment of blood chemistry reference intervals for Arbia goats, including values in phases of reproduction
AbstractThis research was aimed to establish reference intervals of some blood parameters in Arbia goats, reared under a traditional system in the semi-arid region of Algeria during different reproduction periods. Blood samples were collected from 131 Arbia goats before pregnancy (n = 30), during pregnancy (n = 56), and during lactation (n = 45). Compared with reference intervals in available literatures, the plasma concentrations of Glucose, Creatinine, Cholesterol, Total protein, Albumin, Globulin, Alanine aminotransaminase, Phosphorus, Magnesium, Potassium, Sodium, and Chloride were within at least one of them, but Iron...
Source: Comparative Clinical Pathology - May 18, 2021 Category: Pathology Source Type: research

Effect of 1  mg/kg oral prednisolone on biochemical analytes in ten dogs: a cross-over study
AbstractPrednisolone is used for treatment of inflammatory, allergic, neoplastic, and immune-mediated diseases in dogs. As a glucocorticoid, prednisolone has biochemical effects, which may interfere with the interpretation of biochemistry test results. The aim of this study is to investigate the effects of prednisolone treatment in an anti-inflammatory dose on common biochemical analytes in dogs and to evaluate the clinical relevance of the changes. Ten beagle dogs, enrolled in a cross-over study, were treated with oral prednisolone (1  mg/kg 24 h) for 10 days. Blood samples were collected at day 0, 1, 3, 6, 9, 1...
Source: Comparative Clinical Pathology - May 11, 2021 Category: Pathology Source Type: research

Topical application of Azadirachta indica improves epidermal wound healing in hyperglycemic rats
AbstractDiabetes mellitus, which is characterized by hyperglycemia, leads to poor wound healing.Azadirachta indica has antidiabetic properties and further promotes the healing of epidermal wounds. Here, we investigated the effects of topical application ofA. indica extract on excisional lesions in hyperglycemic rats. The animals were divided into four groups (n=15): non-hyperglycemic and treated with carbopol gel (NH), non-hyperglycemic and treated withA. indica + carbopol gel (NHT), hyperglycemic and treated with carbopol gel (H), and hyperglycemic and treated withA. indica + carbopol gel (HT). Lesions were made on the an...
Source: Comparative Clinical Pathology - May 10, 2021 Category: Pathology Source Type: research

Melatonin mitigates thyroid dysfunction induced by gestational and lactational exposure to mixture of chlorpyriphos and cypermethrin in male Wistar rats
AbstractOxidative injury is one of the mechanisms involved in thyroid dysfunctions following pesticide exposure. The present study evaluated the mitigating effect of melatonin (ML) on thyroid dysfunctions induced by gestational and lactational exposure to chlorpyriphos (CP) and cypermethrin (CY) in male rats. Six groups of 5 male rats in each group were exposed in utero and via lactation up till postnatal day (PND) 21 to the following regimens: DW, SO, and ML groups were administered distilled water (2  ml/kg), soya oil (2 ml/kg), and ML (0.5 mg/kg) while CM group was co-administered with CP (1.9 mg/kg)...
Source: Comparative Clinical Pathology - May 7, 2021 Category: Pathology Source Type: research

The effectiveness of different culture media on the growth and proliferation of Acanthamoeba T4 genotype
AbstractAcanthamoeba spp. is one of the free-living amoebas in the environment that many studies focused on. This parasite is of veterinary and public health importance. The present study aimed to evaluate several available media forAcanthamoeba spp. culture to find a suitable axenic liquid medium. In the present study four culture media, including TYM, RPMI-1640, and hydatid cyst fluid (HCF) as well as non-nutrient agar (NNA) as the control group, were examined to evaluate the proliferation ofAcanthamoeba T4 genotype at 24, 48, and 72 h. Based on our results, at 24 and 48 h, the highest number of cysts was observed in TYM...
Source: Comparative Clinical Pathology - May 4, 2021 Category: Pathology Source Type: research

Hypolipidemic activity of an ethanolic extract of quinoa seeds in Triton X-100-induced hyperlipidemic rats
The objective of this work was to investigate the hypolipidemic effects of an ethanolic extract of quinoa seeds (QSEE) in countering hyperlipidemia induced by Triton X-100 (T.  X-100) rats. The study assessed alterations in growth performance and several biochemical parameters. Forty clinically healthy male Albino rats were equally divided into four groups: control animals; hyperlipidemic animals treated with a single intraperitoneal injection of T. X-100 on day 21; rat s administered QSEE for 40 days; and rats receiving a combination of both treatments. Gas chromatography-mass spectrometry (GC-MS) analysis was u...
Source: Comparative Clinical Pathology - April 30, 2021 Category: Pathology Source Type: research

Orally administered gold nanoparticles caused mild oxidative stress in the lungs and liver of Wistar rats
This study therefore investigated the biochemical effects of gold nanoparticles in Wistar rats. Sixteen (16) Wistar rats were grouped into 4 (n = 4). Animals in the negative control group were orally administered 0.3 ml of distilled water (vehicle) while other treatment groups were respectively given oral administration of 0.3 ml each of 1, 10, and 20 mg/kg b.w. AuNPs for 7 days. Biochemical measurements of oxidative stress indices in rat plasma and tissue homogenates were recorded on a UV/Vis spectrophotometer. There was no significant (p> 0.05) difference in the plasma and brain for all the biochemical parameters meas...
Source: Comparative Clinical Pathology - April 21, 2021 Category: Pathology Source Type: research

Long-term successful management of an idiopathic interstitial pyogranulomatous/granulomatous dermatitis and folliculitis by omega 3 fatty acid in a dog
In conclusion, it appears that omega 3 fatty acid was effective for the long-term successful management of an idiopathic interstitial pyogranulomatous/granulomatous dermatitis and folliculitis without other parenteral administration in a dog. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 15, 2021 Category: Pathology Source Type: research