Sodium butyrate attenuates aortic lipotoxicity and impaired nitric oxide synthesis in female Wistar rats exposed to high-fructose drink
This study aimed at investigating the effect of sodium butyrate (NaB) on selected markers of endothelial function and aortic lipotoxicity in female Wistar rats. Twenty female Wistar rats with body weight 120 –125 g were divided after a week of acclimatization into 4 groups of 5 rats each: control (CTR; normal chow), HFr drink: 10% fructose (w/v), NaB (200 mg/kg;po), and NaB + HFr. The administration was carried out for 6 weeks. The animals were euthanized with ketamine hydrochloride and blood samples were collected by cardiac puncture. Aortic tissue ring was excised and homogenized for biochemical parameters usin...
Source: Comparative Clinical Pathology - October 14, 2023 Category: Pathology Source Type: research
Sex-related changes in renal function markers, electrolytes and hematological indices of rats of different age groups in relation to protein excretion
This study aimed to determine the sex-related changes in renal function markers, electrolytes and hematological indices of rats of different age groups in relation to protein excretion. Fifty (50) male and female Wistar rats aged 1, 3, 6, 9 and 12 months were used for the study. At the end of th e different ages being studied, urine samples of rats were collected over a 24-h period using metabolic cages. Thereafter, the rats were sacrificed under ketamine anesthesia and blood was collected into fluoride oxalate and potassium-EDTA bottles for the assay of renal function markers, electrolytes and hematological indices resp...
Source: Comparative Clinical Pathology - October 6, 2023 Category: Pathology Source Type: research
Prediction of postpartum subclinical hypocalcemia using prepartum serum macromineral concentrations in Saanen and Beetal dairy goats
AbstractThe Saanen and Beetal goats are currently the most popular dairy goat breeds in many countries, including Iran. Subclinical hypocalcemia is common in dairy goats during the first few days of lactation similar to that in cattle. The aim of the study was to predict postpartum subclinical hypocalcemia in dairy goats using prepartum serum magnesium, inorganic phosphorous, and potassium concentrations. Blood samples from 280 Saanen and 340 Beetal dairy goats were collected 10 days before and 1 day after parturition. Total serum calcium, magnesium, phosphorous, and potassium concentrations were quantified. The resul...
Source: Comparative Clinical Pathology - October 4, 2023 Category: Pathology Source Type: research
Molecular detection of Chlamydia abortus and investigation of oxidative stress parameters in sheep fetuses in the Black Sea Region of T ürkiye
ConclusionsCP, MDA, TOS and OSI levels were found to be significantly higher (P < 0.05) in theC. abortus infected group compared to the control group (P < 0.05). It was determined that the TAS level decreased in the infected group compared to the control group, but there was no statistically significant difference (P > 0.05). As a result, it was determined thatC. abortus was found at a substantial rate in small ruminant abortion cases in the Black Sea Region. In addition, it was determined for the first time in T ürkiye that the oxidative stress increased in abortions caused byC. abortus and inf...
Source: Comparative Clinical Pathology - September 27, 2023 Category: Pathology Source Type: research
Cardioprotective and renoprotective effects of melatonin and vitamin E on fluoride-induced hypertension and renal dysfunction in rats
AbstractFluoride is an important toxicological and environmental toxicant that is implicated in diverse cardiorenal system dysfunctions via the induction of oxidative stress. The present study aims at evaluating the cardioprotective and renoprotective effects of melatonin and vitamin E on fluoride toxicity on biomarkers of oxidative stress, clinical pathology, and their molecular mechanism of action. Apparently healthy male rats of the Wistar strain (n = 50; 160 ± 7.5 g), were randomly distributed into five groups of ten animals per group as follows: Control, sodium fluoride (NaF, 25 mg/kg), NaF and melatonin 20...
Source: Comparative Clinical Pathology - September 13, 2023 Category: Pathology Source Type: research
The therapeutic potential of “Crataegus azarolus” on zinc, lipid profile, and antioxidant status in streptozotocin-induced diabetic rats with zinc-deficient diet
AbstractZinc plays an important role in the biosynthesis and storage of insulin. Consequently, its deficiency may have a deleterious impact on the progression of diabetes and its associated consequences. Thus, this study was conducted to investigate the effect of hawthornCrataegus azarolus on blood biochemical parameters, tissue zinc status, and oxidative stress biomarkers in streptozotocin-induced diabetic rats fed a zinc-insufficient diet. Thirty-two male albino Wistar rats were divided into four groups: two groups were fed a zinc-sufficient diet (one non-diabetic and the other diabetic), while the other two groups of di...
Source: Comparative Clinical Pathology - September 7, 2023 Category: Pathology Source Type: research
Pregnancy toxemia in Rembi ewe: a case report
AbstractPregnancy toxemia is one of the most common metabolic disorders in ewes. Widely appearing during the late stage of gestation when there is an increase in energy demand due to rapid fetal udder development and colostrum production, this disease can have a high impact on economic losses such as fetal loss and maternal mortality. A 6-year-old ewe of theRembi breed was brought for treatment at the Tiaret Institute of Veterinary Sciences. She was at the last 7 days of pregnancy, fed with barley and bran, and presented with anorexia, weakness, staggering gait, dyspnea, and a loss of reflex. Biochemical analyses showed an...
Source: Comparative Clinical Pathology - September 6, 2023 Category: Pathology Source Type: research
Evaluation of combined antioxidant potential of p-coumaric acid and/or lisinopril — in vitro and in vivo
This study was convened to determine the combined antioxidative effect ofp-CA and/ lisinopril — in vitro and in vivo. Stock solutions ofp-CA (50 mM) and lisinopril (50 mM) were separately prepared. Subsequently, preparation of different combinations of samples (50%p-CA: 50% lisinopril; 75%p-CA: 25% lisinopril; 25%p-CA: 75% lisinopril, 100%p-coumaric acid and 100% lisinopril) was carried out for the determination of in vitro antioxidant properties as typified by DPPH, ABTS, and hydroxyl radical scavenging abilities, Fe2+ chelating ability, reducing property, and inhibition of Fe2+-induced lipid peroxidation. Furthermor...
Source: Comparative Clinical Pathology - September 1, 2023 Category: Pathology Source Type: research
Alteration in apoptosis and ataxia telangiectasia mutated (ATM) gene expression in mesenchymal stem cells in patients with idiopathic acquired aplastic anemia
AbstractAcquired aplastic anemia (AA) is a chronic debilitating disease, often fatal with no effective treatment. Intense research into its pathogenesis is ongoing with promising results. The present study explores apoptosis and ATM gene expression in mesenchymal stem cells (MSCs) in idiopathic aplastic anemia (IAA). The study included 15 acquired IAA patients and 17 patients with non-aplastic non-neoplastic disorders and matched age and sex distribution as a control group. Long-term bone marrow (BM) cultures for patients and controls followed for isolation of MSCs isolation and their flow cytometry identification using CD...
Source: Comparative Clinical Pathology - August 31, 2023 Category: Pathology Source Type: research
Cement dust inhalation induces hepato-renal dysfunction via tissue heavy metal bioaccumulation, histopathological and biochemical mechanisms
AbstractSystemic degradations of various forms have been adduced to cement dust exposure and Inhalation is the most considerable route. We deployed inhalation method to investigate the possible reno-hepatic effect of cement dust exposure. Thirty male Wistar rats weighing between 150 and 180g acquired were acclimatized and randomized into three groups of 10 animals each. Group 1 was the control, group 2: 14-day exposed, and group 3: 28-day exposed. Relative organs weights, heavy metal analysis, hepatic and renal functions tests, fibrotic index, lipid peroxidation (MDA), superoxide dismutase (SOD), catalase (CAT), nitric oxi...
Source: Comparative Clinical Pathology - August 31, 2023 Category: Pathology Source Type: research
Clinical, haematological, and biochemical changes in buffaloes with natural Anaplasma infection
AbstractReports and documentation were very limited on buffaloes with naturalAnaplasma infection in India. The present research was undertaken to record the prevalence, clinical signs, haematological, and serum biochemical alterations in buffaloes with anaplasmosis at College of Veterinary Sceince, Proddatur, Andhra Pradesh from January 2022 to December 2022. Buffaloes with signs of fever, emaciated body condition, lymphadenopathy, and congested mucus membranes were selected for the study. Peripheral blood smears, whole blood, and serum were collected for l aboratory analysis. Confirmation of the anaplasmosis was done b...
Source: Comparative Clinical Pathology - August 31, 2023 Category: Pathology Source Type: research
Gentamicin-induced acute nephrotoxicity counteraction using Boerhaavia diffusa in Swiss albino mice
AbstractDisruption of homeostasis, detoxification and excretion in kidney functions by endogenous or exogenous toxicants results in the occurrence of nephrotoxicity. The study aimed to exploreBoerhaavia diffusa L., a Nyctaginaceae herbal medicine that has been used commonly for nephrotic syndrome for its nephroprotective property against gentamicin-induced nephrotoxicity. Swiss albino mice were administered with gentamicin and pre-treated withB. diffusa. Biochemical aspects and antioxidants of the kidney were estimated. Histological impacts along with expressions of kidney injury markers and mediators were examined. The ph...
Source: Comparative Clinical Pathology - August 30, 2023 Category: Pathology Source Type: research
The impact of aerobic exercise training and crocin consumption on MuRf-1 level in skeletal muscle in high-fat fed ovariectomized rat models
AbstractEstrogen deficiency intensifies sarcopenia by activation of inflammatory and oxidative pathways. Aerobic exercise training and antioxidant/inflammatory supplements might reverse such outcomes or decline the degenerative pathway. Thus, we investigated the impact of aerobic training and crocin (CRO) consumption on skeletal muscle MuRf-1 and plasma antioxidant and inflammatory statues in ovariectomized (OVX) rat models fed on a high-fat diet (HFD). 72 female Wistar rats (200 ± 20 gr) were randomly divided into 9 groups: (1) non-OVX + standard chow (nOVX + SC), (2) nOVX + HFD, (3) high-intensity conti...
Source: Comparative Clinical Pathology - August 25, 2023 Category: Pathology Source Type: research
Nano-chitosan/bovine lactoperoxidase and lactoferrin formulation modulates the hepatic deterioration induced by 7,12-dimethylbenz[a]anthracene
This study aimed to evaluate the therapeutic potential of a prepared nano-combination composed of LPO and LF loaded on chitosan nanoparticles (LPO + LF/CNPs) against oxidative damage in the liver of rats exposed to 7,12-dimethylbenz[a]anthracene (DMBA). Twenty-four male Wistar albino rats (4 months old) were divided into three equal groups and treated orally as follow: (1) rats were given saline and served as control, (2) rats received a single dose (20 mg/kg) of DMBA dissolved in soy oil, and (3) rats intoxicated with a single dose (20 mg/kg) of DMBA, 56 days prior to their oral treatment with the nano-combinatio...
Source: Comparative Clinical Pathology - August 18, 2023 Category: Pathology Source Type: research
Investigation of some acute phase protein levels in cattle infected with Brucella abortus
AbstractBrucellosis is a chronic zoonotic disease caused by brucella strains that adversely affects human and animal health. Increases or decreases in acute phase protein (APP) levels are observed in various infective diseases in cattle. In our study, it was aimed to investigate how the change in APP levels in cattle infected withBrucella abortus and which ones can be used as a marker. In the study, blood serum and plasma of 28 brucella and 10 healthy cattle collected from various cattle farm in Samsun were used. Rose bengal plate test and complement fixation test were applied to the serums. While serum amyloid A (SAA), ha...
Source: Comparative Clinical Pathology - August 12, 2023 Category: Pathology Source Type: research
