Comparison of the effects of two methods of euthanasia on post mortem changes in rats: histopathological and molecular findings
AbstractPostmortem changes (PMCs) not only affect the results of experimental studies, but also determine postmortem intervals in forensic sciences. The present study aimed to assess the effects of two methods of laboratory animals ’ euthanasia on PMCs in rats. In this experimental study, 10 female rats were randomly assigned to two equal groups and were euthanized using the inhalation of CO2 (gas-treated group) or over-dose intramuscular injection of ketamine/xylazine (drug-treated group). Kidney and liver tissue samples were collected at baseline and 2, 4, 6, 8, 10, 12, 24, and 48 h after euthanasia and were subjecte...
Source: Comparative Clinical Pathology - August 10, 2022 Category: Pathology Source Type: research
Acute toxicity study of Guiera senegalensis J.F. Gmel methanolic leaf extract in Wistar albino rats through oral administration
AbstractThe abundance of medicinal plants does not guaranteed that they are safe, as there is dearth of data on their toxicity and safety. The present study evaluated the oral acute toxicity of methanolic leaf extract ofGuiera senegalensis J.F. Gmel (GS), a plant used traditionally for the treatment of epilepsy. In phase one, 12 rats were divided in to 4 groups (n = 3) and orally administered with water, 10 mg/kg, 100 mg/kg, and 1000 mg/kg of GS respectively. In phase two, four rats were divided into four groups (n = 1) and orally administered with water, 1600 mg/kg, 2900 mg/kg, and 5000 mg/kg of GS extract...
Source: Comparative Clinical Pathology - August 10, 2022 Category: Pathology Source Type: research
Significance of LINC00641 and miR-378 as a potential biomarker for colorectal cancer
AbstractIn colorectal cancer (CRC), dysregulation of noncoding RNA expression is a distinguishing factor. Owing to the conflicting results and the insufficient studies on serum microRNA-378 (miR-378) and long intragenic noncoding RNA00641 (LINC00641) expression patterns, we aimed to explore their expression profiles and diagnostic ability in colorectal cancer. Blood samples were collected from 30 healthy controls and 70 CRC patients. miR-378 and LINC00641 expression levels were determined using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), carcinoembryonic antigen (CEA) levels assessed...
Source: Comparative Clinical Pathology - August 9, 2022 Category: Pathology Source Type: research
TEG assay to assess the altered lipid profile in patients with type 2 diabetes mellitus
This study aimed to investigate the effect of lipid profile in patients with type 2 DM toward hemostasis profile using TEG assay. A cross-sectional study was conducted on patients with type 2 DM in the Endocrinology Outpatient Clinic of Saiful Anwar Hospital from November 2020 to April 2021. We measured the serum level of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TG) using an automatic analyzer. TEG is a diagnostic method to identify hemostatic profiles, including primary, secondary, and tertiary hemostasis. This study used at-test and correlation-regression t...
Source: Comparative Clinical Pathology - August 8, 2022 Category: Pathology Source Type: research
Hematological changes during pregnancy and lactation in Pelibuey ewes infected with gastrointestinal nematodes
The objective was to determine the hematological parameters associated with physiological stages, type of lambing, age, and susceptibility in ewes infected with gastrointestinal nematodes (GIN). Forty-six Pelibuey ewes of 1.5 –4 years of age and naturally infected with GIN were sampled during pregnancy and lactation. Fecal nematode egg counts (FECs) were determined and blood samples obtained to determine the hematocrit (HCT), peripheral eosinophil count (PEC), total plasma protein (TPP), and immunoglobulin A level (Ig A) in plasma. Hematological variables were measured in a MEDONIC analyzer. The variables were analyzed ...
Source: Comparative Clinical Pathology - August 6, 2022 Category: Pathology Source Type: research
Clinical, haemato-biochemical and therapeutic aspects of viperine snakebite in a mare
Abstract
Venomous snakebites due to various poisonous snakes in equines are seldom reported. The present report describes the case of viperine snakebite in a mare. A 2.5-year-old mare was admitted to clinics with history of swelling over right forelimb, bleeding from limb, anorexia and lameness. Clinical examination of bitten mare showed ascending swelling in right forelimb, lameness, bleeding point and fang mark at the site of bite. Vital parameters showed normal body temperature with tachycardia and tachypnoea. Haematological examination revealed granulocytic leukocytosis, thrombocytopenia and prolonged capillary bloo...
Source: Comparative Clinical Pathology - August 2, 2022 Category: Pathology Source Type: research
Comparison of day 21 vaginal exfoliative cytology lavage and vaginal histology in the evaluation of oestrous cycle stages in group-housed CD-1 mice
AbstractVaginal lavage cytology is commonly used in rodents to determine oestrous cyclicity and is a requirement for regulatory reproductive toxicity studies. While rats are the preferred species for these studies, where scientifically justified, the mouse is an alternative species. The current study sought to evaluate the utility of day 21 vaginal lavage cytology in the CD-1 mouse for assessment of changes in the murine oestrous cycle. It compared the vaginal lavage technique, using either fresh or fixed and stained cytology smears, with the histological evaluation of the vagina on day 21 of a murine study and examined th...
Source: Comparative Clinical Pathology - August 2, 2022 Category: Pathology Source Type: research
Enteric pathogenic protozoa from misdiagnosis to overmedication in Egypt: a need for molecular diagnosis
AbstractCryptosporidium spp.,Entamoeba histolytica (E. histolytica), andGiardia intestinalis (G. intestinalis) are prevalent in developed and developing countries, including Egypt. These enteric protozoa usually provoke indistinguishable manifestation including diarrhea. There is scarce studies of their true prevalence in Egypt. We aimed to identify the accurate detection of these enteric pathogenic protozoa from human diarrheic samples using a multiplex-PCR (MT-PCR) assay and to evaluate the diagnostic performance of coproscopy and monoplex nested PCR (nPCR). Fecal specimens were collected from 100 diarrheic patients and ...
Source: Comparative Clinical Pathology - July 28, 2022 Category: Pathology Source Type: research
Anti-inflammatory activity of combined pods and seed extract of Parkia Speciosa on lipopolysaccharide-induced paw edema in rats
This study provided a report on the selected medicinal use ofParkia speciosa Hassk or stink beans in terms of its anti-inflammatory activity. Previous studies related to its anti-inflammatory activity only focused onin vitro studies. Due to the lack of previous in vivo studies, the data in this study supported the findings by previous researchers and facilitated future drug discovery research.Lipopolysaccharide (LPS)-induced paw edema was studied in this article. Subplantar injection of LPS, 0.1 ml with the concentration of 100 µg/100 µL into the paw of rats elicited an acute inflammatory response characterized by an in...
Source: Comparative Clinical Pathology - July 22, 2022 Category: Pathology Source Type: research
Cytology of bone marrow in acute, sub-acute and chronic CCL4-toxicity in female Wistar rats
This study is a look at bone marrow responses to acute, sub-acute and chronic CCl4 toxicities in female Wistar rats. Sixteen (16) female Wistar rats, with a mean body weight of 180 g, were used for this study. The rats were randomly allocated into four groups of four rats each. Group I served as the control and was untreated. Group II was the acute study group and received 20% CCl4 at 3 mL/kg body weight, intraperitoneally (i.p.), stat. Group III was the sub-acute study group and received 15% CCl4 at 3 mL/kg body weight, i.p. every 5 days for 28 days, while group IV rats were the chronic study group and received 10...
Source: Comparative Clinical Pathology - July 21, 2022 Category: Pathology Source Type: research
Effect of composition of medicinal plants on growth performance, gut bacteria, hematological parameters, anticoccidial index, and optimum anticoccidial activity in domestic chicken
In this study, we evaluated the impact of two edible multi-plant extract compounds on growth performance, gut bacteria, and hematological parameters, anticoccidial index (ACI), and optimum anticoccidial activity (OAA) in 17-day-old domestic chickens experimentally infected with protozoanEimeria. The highest weight gain was recorded in the uninfected unmedicated group followed by a mixture of medicinal plants 0.01 (T3) group. The basophil and total red blood cells were significantly higher in control groups in comparison with those in all other groups, and the total white blood cells were significantly lower in control grou...
Source: Comparative Clinical Pathology - July 16, 2022 Category: Pathology Source Type: research
Impact of three anticoagulants and their storage time on hematological parameters of snow trout, Schizothorax labiatus, habiting in river Sindh of Indian Himalayan region
AbstractBlood analysis is a critical and potent diagnostic tool used in the field of fisheries to determine the well-being status of the fish. However, internal and external influences, such as anticoagulants and storage time, can have a significant impact on fish hematological values resulting in species-specific reactions. Because of the short clotting periods, fish blood generally needs the inclusion of an anticoagulant agent; therefore, choosing the right anticoagulant is imperative for achieving accurate blood test results. The aim of current study is to analyze the impact of three different anticoagulants (lithium he...
Source: Comparative Clinical Pathology - July 13, 2022 Category: Pathology Source Type: research
Trial for decreasing ifosfamide-induced hematological toxicity, oxidative stress, inflammation, and hepatotoxicity by beetroot extract in male albino rats
AbstractHepatotoxicity restricts the therapeutic benefit of many anti-tumor agents in clinic. Ifosfamide is a widely used antineoplastic drug which could cause toxicity of normal cells due to its toxic metabolites mainly via induction of oxidative stress. Regarding established antioxidant and anti-inflammatory potential of beetroot extract, we aimed to investigate its possible protective effect against ifosfamide-induced hepatotoxicity in the rat model through hematological, histopathological, biochemical, and immunohistochemical evaluation of oxidative and inflammatory biomarkers. A total of twenty-four male albino rats w...
Source: Comparative Clinical Pathology - June 29, 2022 Category: Pathology Source Type: research
The prevalence, mechanism of action, and toxicity of Nigerian psychoactive plants
AbstractCannabis sativa, Datura stramonium, Nicotiana tabacum, andCarica papaya are plants that naturally grow in Nigeria. They are reportedly rich in neuroactive compounds that are capable of reacting with the nervous system to elicit psychoactive and/or toxic effects that deter predators. However, despite the toxicological potential of these plants, their recreational use is on the rise due to the psychoactivity they proffer and prevalence in Nigeria. The aim of the present study is to evaluate the plants ’ recreational use, mechanism of actions and toxicities. Relevant published documents on psychoactive plants in Nig...
Source: Comparative Clinical Pathology - June 28, 2022 Category: Pathology Source Type: research
Cold shock and its effect on biochemical indices, cortisol and electrolyte changes in Chao Phraya catfish, Pangasius sanitwongsei Smith, 1931
AbstractThe aim of this research was to study the influences of cold shock on some biochemical and physiological changes inPangasius sanitwongsei. To simulate a stressor, four different water temperature levels (25 °C, 25–22 °C, 25–17 °C, and 25–15 °C) were used. Results showed that cold shock affected biochemical and physiological indices. The highest levels of ALT and glucose were found in the group subjected to 25–17 °C; meanwhile, the lowest AST was observed in a group exposed to 25–22 °C. The highest cortisol level was observed in the group exposed to 25–15 °C. The highest and the lowest le...
Source: Comparative Clinical Pathology - June 28, 2022 Category: Pathology Source Type: research
