Investigation of NDM metallo-beta-lactamase and CMY-2 AmpC β-lactamase production in Escherichia coli and Enterobacter spp. isolated from human
AbstractNew Delhi metallo-beta-lactamase (NDM) and CMY-2-AmpC β-lactamase are among the most important resistance factors against carbapenem and beta-lactam antibiotics. Considering the few studies in Iran, here NDM and CMY-2 production inEscherichia coli (E.coli) andEnterobacter isolates were evaluated. Acquired resistance profiles of isolates were determined based on the new definitions as multi-drug resistant (MDR), extensively drug-resistant (XDR) and pan-drug resistant (PDR). In carbapenem-resistant strains, minimum inhibitory concentration (MIC) of different antibiotics by Etest method and the clonal relationshi...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Haematological changes in dogs with prolonged administration of high doses of sulphamethoxazole/trimethoprim
AbstractSulphamethoxazole/trimethoprim (SMX/T) is an antimicrobial agent that has been used for decades against both Gram-positive and Gram-negative bacteria as well as protozoa. It is particularly used as prophylaxis againstPneumocystis carinii pneumonia (PCP) in patients with acquired immune-deficiency syndrome (AIDS). Its use in dogs with associated adverse reactions has necessitated an experimental study that may enhance clinical management of the toxicities. The study aimed to determine the effects of prolonged treatment with high doses of sulphamethoxazole/trimethoprim on the haematological profile of dogs. Dogs were...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Human MTHFR -C677T gene transition increased risk of Alzheimer disease: a case-control study which performed by optimized PCR-CTPP method
AbstractMethylenetetrahydrofolate reductase (MTHFR) is a gene involved in folate metabolism which may contribute to the risk of Alzheimer disease (AD). In this paper, an association ofMTHFR-C677T single nucleotide polymorphism (SNP) with the AD was evaluated by optimized PCR-confronting two-pair primers (PCR-CTPP) genotyping method. For this aim at first, PCR-CTPP method was optimized for detection of this SNP by additive agents and touchdown PCR. Then, an association of the mentioned SNP with the AD was assessed in a case-control study comprising 113 Alzheimer ’s patients and 123 controls. Genotyping results showed ...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Investigation of NDM metallo-beta-lactamase and CMY-2 Amp. β-lactamase production in Escherichia coli and Enterobacter spp. isolated from human
AbstractNew Delhi metallo-beta-lactamase (NDM) and CMY-2-AmpC β-lactamase are among the most important resistance factors against carbapenem and beta-lactam antibiotics. Considering the few studies in Iran, here NDM and CMY-2 production inEscherichia coli (E.coli) andEnterobacter isolates were evaluated. Acquired resistance profiles of isolates were determined based on the new definitions as multi-drug resistant (MDR), extensively drug-resistant (XDR) and pan-drug resistant (PDR). In carbapenem-resistant strains, minimum inhibitory concentration (MIC) of different antibiotics by Etest method and the clonal relationshi...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Seasonal modulation of reproductive hormones and related biomarkers in coldwater cyprinid Barilius bendelisis (Hamilton, 1807)
AbstractBarilius bendelisis is a valuable ornamental and food fish from India in which the endocrine control of its reproduction activity has not been widely reported. The present study was aimed to elucidate changes in serum endocrine hormones: 17 β-estradiol (E2), testosterone (T), progesterone (P4), 17 α, 20β-dihydroxyprogesterone (17α, 20β-P), cortisol (C), triiodothyronine (T3), and thyroxine (T4); aromatase activity (ARO), vitellogenin (VTG), and total antioxidant capacity (TAC) during different seasons of the year 2014. All studied parameters showed marked seasonal variations. The highest ...
Source: Comparative Clinical Pathology - March 6, 2018 Category: Pathology Source Type: research

Endocrine disruption induced by bisphenol A in young and adult female Sprague Dawley rats
In conclusion, BPA exposure resulted in endocrine disruption indicated by different responses in hormonal and histopathological changes in both young and adult femal e rats. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 5, 2018 Category: Pathology Source Type: research

Acute toxicity and histopathological changes in livers of frog tadpoles ( Hoplobatrachus rugulosus ) exposed to bioinsecticides derived from Azadirachta indica A. Juss., Stemona curtisii Hook.F., and Mammea siamensis
AbstractAzadirachta indica A. Juss.,Stemona curtisii Hook.F., andMammea siamensis are popularly used as bioinsecticides by Thai farmers. To evaluate their safety on frog (Hoplobatrachus rugulosus) tadpoles, the ecologically and economically important species of Thailand, an acute static toxicity test was performed. The tadpoles (n = 10 per treatment group) were exposed to the bioinsecticides fromA. indica (NSAI 5, 10, 15, 20, and 25  g/l) and the mixture ofS. curtisii andM. siamensis (SCMS 0.8, 1.2, 1.6, 2.0, and 2.4  mg/l). It was found that the median lethal concentration (LC50) values at 96  ...
Source: Comparative Clinical Pathology - March 3, 2018 Category: Pathology Source Type: research

CD58; leucocyte function adhesion-3 (LFA-3) could be used as a differentiating marker between immune and non-immune thyroid disorders
AbstractThe link between Graves ’ disease (GD) and Hashimoto’s thyroiditis (HT) has been debated for decades due to the shared pathological and immunological components. Immune intolerance and inappropriate immune reaction against self-thyroid cells are distinctive features of both diseases, but definitive data for the clinical presentation of autoimmune thyroid disease remains unclear. To analyse the expression of T-regulatory cells, CD58, the CD4/CD8 ratio and the neutrophil/lymphocyte ratio and to determine if these parameters could be used as differentiating markers between auto- and non-immune thyroid dise...
Source: Comparative Clinical Pathology - March 3, 2018 Category: Pathology Source Type: research

Assessment of antioxidant and neuroprotective activities of methanol extract of Moringa oleifera Lam. leaves in subchronic chlorpyrifos-intoxicated rats
In conclusion, MO pretreatment reduced brain oxidative damages in rats sub-chronically-exposed to CPF. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 2, 2018 Category: Pathology Source Type: research

The impacts of yoghurt butter oil on rat testicular morphology and sexual hormones in a 150-day study
AbstractThe oil derived from traditional produced yoghurt has received much attention over the last two decades. While a high-fat diet (HFD) is assumed to be related to reproductive impairments, fermented dairy products are believed to have positive effects on the regulation of HFD-induced male reproductive damage. The reproductive effects of sheep ’s yoghurt butter oil (SYBO) have never been studied yet. The current study was to seek for the scientific evidence to determine SYBO effects on male rat reproductive system. In the present study, male Wistar rats were treated by standard diet or standard diet supplemented...
Source: Comparative Clinical Pathology - March 1, 2018 Category: Pathology Source Type: research

Evaluation of Houttuynia cordata Thunb. leaf extract on growth performance, feed utilization, and hematological indices of hybrid catfish ( Clarias macrocephalus × Clarias gariepinus )
AbstractThis research evaluates the effects ofHouttuynia cordata thunb. leaf extract on growth performance, feed utilization and the hematological indices of Hybrid catfish (Clarias macrocephalus×Clarias gariepinus). Four groups of fish were divided and given a basal diet (control group) as well as the extract solution, which was sprayed into the commercial fish feed (30% crude protein) in the amounts of the extract, 50, 70 and 100  ml/kg feed, then fed to the experimental fish for 90 days. Dominant results for the growth indices were found on days 15 and 30 of the experimental period. A significantly incre...
Source: Comparative Clinical Pathology - February 27, 2018 Category: Pathology Source Type: research

Effects of electrofishing on stress response of wild carp ( Cyprinus carpio , L.)
AbstractElectrofishing is commonly used by fishery scientists for collecting fishes. During such work, fishes are often exposed to air for several minutes which lead to deleterious impacts. The purpose of the current paper was to compare hematological and biochemical parameters of wild carp when electrofishing device was applied. Hematology and serum biochemical analyses were performed. The result showed significant differences (p 
Source: Comparative Clinical Pathology - February 27, 2018 Category: Pathology Source Type: research

Histomorphometric and ultrasonographic evaluations of the abomasum in sheep
In this study, the samples were collected from fundus, body, and pyloric regions of the abomasum from 10 healthy sheep and fixed in 10% neutral buffered formalin. All samples were processed through routine paraffin embedding technique, cut at 6  μm, and stained with hematoxylin and eosin. Histomorphometry of samples was done by Olympus microscope (BX1) and camera (DP25). Ultrasonography of the abomasum wall was performed with 8-MHz probe. Statistical analysis showed that there is no significant correlation between ultrasonography and his tomorphometry. It seems possible that these results are due to unwanted change...
Source: Comparative Clinical Pathology - February 26, 2018 Category: Pathology Source Type: research

Prognostic relevance of CD69 expression in B cell chronic lymphocytic leukemia
This study aimed to determine the prognostic impact of CD69 expression in B cell chronic lymphocytic leukemia (CLL). The study included 153 B cell chronic lymphocytic leukemia (B-CLL) recruited to Mansoura Oncology Center. Cellular CD69 expression was determined by multicolor flow cytometry in parallel with PCR amplification and sequencing analysis to mutational status of immunoglobulin variable heavy chain (IgVH). The number of CLL patients positively expressed CD69 was 47 out of 153 (30.7%). There is significant association between CD69-positive expression and unmutated IgVH and between CD69 negative expressions with mut...
Source: Comparative Clinical Pathology - February 26, 2018 Category: Pathology Source Type: research

Concurrent infection of Maedi-Visna with lung worms and pulmonary adenomatosis in sheep kept in Debre Berhan Sheep Improvement Station, Central Ethiopia
AbstractThe study was conducted with the objectives of determining the possible causes of pneumonia in the sheep and to characterize the microscopic lesions of concurrent infection of Maedi-Visna with lung worms and other infectious pneumonia. Detailed gross and microscopic examinations were employed on 35 Maedi-Visna seropositive sheep. Out of 35 sheep slaughtered and examined for gross lesions on their lungs, lung worms, fibrinous adhesions, and mottling on the cranioventral lobes were observed with decreasing order of frequency. Histopathologically, the pneumonic lungs (n = 35) revealed Maedi (100%), Maedi-V...
Source: Comparative Clinical Pathology - February 26, 2018 Category: Pathology Source Type: research

Innate immunological responses of Asian sea bass, Lates calcarifer (Bloch, 1790) for experimentally challenged Aeromonas hydrophila infection
AbstractModulation of innate immunity was determined in theAeromonas hydrophila-challenged Asain sea bass,Lates calcarifer. The haemato-immunological parameters were studied in blood serum. Blood was sampled at 3, 6, 12, and 24th  h post-challenged infection and control. Histopathological examination of most important organs (ventral musculature tissue, kidney and liver) was studied in the infected fish. The present study results indicated that at early stages of infection, TEC, Hb, PCV, MCV, MCH, and MCHC were not altered significantly, whereas in TLC, the values were fluctuated. Lysozyme, NBT, and protein were signi...
Source: Comparative Clinical Pathology - February 24, 2018 Category: Pathology Source Type: research

The effect of ( Humulus lupulus L.) extract on blood factors in male rat
AbstractHumulus lupulus L. is a plant from cannabinaceac family which is grown in Iran as well as most regions round the world. The present study aimed at examining effects of hydroalcoholic extract ofHumulus lupulus L. hops on blood pictures in male rats. Rats were randomly assigned to seven groups of ten, including one control group (no treatment). All of the six groups under the study were exposed 7  weeks to 25% tween 80° solution (1 ml/kg/day), olive oil (1 ml/kg/day), hydroalcoholic extract ofHumulus lupulus L. (H) at 50, 150, or 450  mg/kg/day, as well as Di Ethyl Stilbestrol (DES) (100 ...
Source: Comparative Clinical Pathology - February 21, 2018 Category: Pathology Source Type: research

Hematological and plasm biochemical values for Rhinogobio ventralis in the Yangtze River, China
This study aimed to establish a “normal” hematological profile for theRhinogobio ventralis and to provide the basis for future comparative surveys. The hematological and plasma biochemical parameters were measured in the cyprinid species,R. ventralis, collected from the upper reaches of the Yangtze River. The concentrations of red blood cell count, white blood cell count, hematocrit, hemoglobin, mean corpuscle volume, mean corpuscle hemoglobin, Cl−, Ca2+, creatinine, urea, glucose, plasma total protein, plasma albumin, globulins, triglyceride, cholesterol, alanine aminotransferase, aspartate aminotransfer...
Source: Comparative Clinical Pathology - February 21, 2018 Category: Pathology Source Type: research

Perturbations of erythrocyte membrane integrity by subchronic low-level lead exposure in New Zealand white rabbits
AbstractLead (Pb) poisoning has been recognised as a clinical condition since ancient times. The anaemia frequently observed in Pb poisoning is thought to result from the shortening of erythrocytes, life span and inhibition of key enzymes of haem synthesis [viz. delta aminolevulinic acid dehydratase (ALAD), pyrimidine 5 ′-nucleotidase type 1 (P5N-1) and ferrochelatase]. However, the exact mechanism by which Pb shortens erythrocyte life span is still debatable. In the present study, the effect of ingested Pb on erythrocyte membrane sialic acid content and osmotic fragility of New Zealand White (NZW) rabbits was st udi...
Source: Comparative Clinical Pathology - February 20, 2018 Category: Pathology Source Type: research

Effect of age, breed and sex on haematological and blood biochemical parameters in helmeted guinea fowl ( Numida meleagris )
This study evaluated the effect of age, breed and sex on haematological and blood biochemical parameters in helmeted guinea fowl. Haemoglobin concentrations increased (P 
Source: Comparative Clinical Pathology - February 19, 2018 Category: Pathology Source Type: research

Recombinant VP2 expressed in baculovirus and adjuvanted with TIR-TLR7: a vaccine candidate against infectious bursal disease virus
AbstractCurrently, a variety of immunostimulatory molecules are used as adjuvants in combination with poor immunogenic recombinant infectious bursal disease virus (IBDV) vaccine antigen. TIR domain of Toll-like receptors (TLRs) triggers the interleukin-1 (IL-1) signaling cascade which plays a critical role in immune responses against infectious agents. For this study, the bacmid DNA pBac-VP2 of a field isolate was generated and transfected to Sf9 insect cells to produce recombinant VP2 (rVP2). In addition, based on in silico analysis, the cDNA encoding the conserved TIR domain of TLR7 (TIR-TLR7) contain the IL-1 receptor w...
Source: Comparative Clinical Pathology - February 19, 2018 Category: Pathology Source Type: research

Ovarian morphology and development of 3 to 8  months old African catfish, Clarias gariepinus
AbstractThirty female African catfish of 3, 4, 5, 6, 7, and 8  months (n = 5 per age) cultured in south-east Nigeria were used to investigate ovarian morphology and development using gross anatomical and histological techniques. The results illustrated that the gonadosomatic indices of the African catfish peaked at 7 months of age (20.05 ± 1.45). Histologicall y, the ovaries of 3-month-old fish exhibited only pre-vitellogenic oocytes, while 4- to 8-month-old fish showed all stages of oocyte development, with predominance of pre-vitellogenic at 4 and 5 months old, and mature...
Source: Comparative Clinical Pathology - February 17, 2018 Category: Pathology Source Type: research

Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch
AbstractThe purpose of this study was to assess the hypoglycemic and protective activities of the ethanolic extract ofAllium saralicum (AS) in streptozotocin (STZ) induced diabetic mice. Diabetes was experimentally induced by intraperitoneal injection of STZ (60  mg/kg bw) in 35 mice. Fasting blood glucose (FBG) levels were measured everyday using glucometer strips. Mice with plasma glucose level> 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into five groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 0.5 ...
Source: Comparative Clinical Pathology - February 17, 2018 Category: Pathology Source Type: research

Hematological and iron-related parameters in a dual-purpose local cattle breed compared to the specialized Italian Friesian breed during transition and lactation periods
AbstractCattle genetic selection has resulted in cows more predisposed to physiological diseases and immunological disorders. The aim of this study was to compare hematological and iron-related parameters during transition and lactation between the dual-purpose local cattle breed Modenese (MO) and the Italian Friesian (IF), housed together under identical conditions of feeding and management. Blood samples from 25 pluriparous lactating cows, 11 MO and 14 IF, were collected at different physiological status: ante-partum (a.p.), early post-partum (p.p.), and lactation. Significant differences were observed during all three p...
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Co-administration of artemisinin and Ricinodendron heudelotii leaf extract —effects on selected antioxidants and liver parameters in male Wistar rats
This study assessed the effect of co-administration of artemisinin andRicinodendron heudelotii extract on certain liver and antioxidant indices in rats. Four groups containing ten rats each were administered distilled water (group A), artemisinin only (group B), artemisinin withR.heudelotii extract (group C), andR.heudelotii extract only (group D). Serum biochemical values and antioxidant parameters were determined using standard methods respectively. The results revealed that the total protein level increased significantly (p 
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Renaut bodies in the hind limb nerves of cattle with downer cow syndrome
AbstractRenaut bodies are loosely textured, whorled, cell-sparse structures that are located in the sub-perineurial area of peripheral nerves. Renaut bodies have been previously recognized in human beings and some animal species. Pathogenesis of the structures is not fully understood. In the present study, peripheral nerves of four adult Holstein-Friesian dairy cows affected with the downer cow syndrome were investigated histopathologically. The animals were culled 3  days (one case), 12 days (one case), or 11 days (two cases) after the onset of clinical signs indicative of motor dysfunction, such as hind li...
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Molecular detection of Leishmania species in northeast of Iran
AbstractTwo known types of cutaneous leishmaniasis (CL) including zoonotic CL due toLeishmania major and anthroponotic CL due toLeishmania tropica are prevalent in 14 of 22 countries located in the Eastern Mediterranean region including Iran. According to existing data, CL is endemic in Sabzevar City (northeast of Iran) and, because of the climatic conditions in this semi-desert region, is suitable for living vector/reservoir hosts of infection. The aim of our study was to identify the recent status of CL causative species in rural areas of Sabzevar County. Suspected patients of CL who were referred to health centers in su...
Source: Comparative Clinical Pathology - February 16, 2018 Category: Pathology Source Type: research

Malignant peripheral nerve sheath tumor in a cat: cytological, histopathological, and immunohistochemical aspects
AbstractThe malignant peripheral nerve sheath tumor is an uncommon tumor in cats. Cytological preparations can be used to diagnose such neoplasia, being a useful tool to characterize a spindle cell tumor presenting several criteria of malignancy. Histopathological examination is required for a definitive diagnosis, complemented by immunohistochemical techniques. This paper describes a case of malignant peripheral nerve sheath tumor in a cat. The diagnosis of mesenchymal neoplasm with high pleomorphism was suggested by cytology, performed through fine needle aspiration. Thereafter, the nodule underwent surgical excision, hi...
Source: Comparative Clinical Pathology - February 15, 2018 Category: Pathology Source Type: research

Haemocytomorphometric variation in different ages of Japanese quail, Coturnix coturnix japonica (Temminck and Schlegel, 1849)
AbstractThe investigation focuses to assess the haematological and cytomorphometric variation in Japanese quail (Coturnix coturnix japonica) at different age groups with sexual dimorphism. Two millilitres of blood from 60 individual birds, comprising 10 males and 10 females from three age groups, was collected from ulnar vein of Japanese quail. Haematological and morphometric values of the blood samples were determined by using standard protocols. For haematology and morphometry, three age groups were considered, i.e., chick (0 –3 weeks), grower (4–5 weeks), and adult (above 5 weeks), respectivel...
Source: Comparative Clinical Pathology - February 14, 2018 Category: Pathology Source Type: research

Potential pharmaceutic effect of Shilajit (mumie) on experimental osteoarthritis in rat
AbstractOsteoarthritis (OA) is a worldwide joint disease with clinically loss of motion and pain in human. The conventional treatments are associated with essential side effects. In folk medicine, Shilajit is applied for treatment of arthritis and bone fractures. The present study evaluated effect of Shilajit on the osteoarthritis in rat model. Thirty-six adult male rats were randomly divided into two groups including OA and treated with Shilajit groups. OA was induced by injection of monosodium iodoacetate in the articular space of femorotibial joint. Aqueous extract of Shilajit was given to the treatment group by gavage ...
Source: Comparative Clinical Pathology - February 14, 2018 Category: Pathology Source Type: research

Biochemical and ultrasonographic evaluation of exertional rhabdomyolysis and myositis in draft horses
AbstractThe purpose of this study was to evaluate the biochemical alterations in horses with exertional rhabdomyolysis (ER) and those with myositis and to characterize the progressive changes of both diseases by using ultrasonography (US). For this reason, 40 horses with various symptoms of musculoskeletal disorders were studied. Based on the competent case history, clinical symptoms, and in combination with clinicopathological findings as well as ultrasonographic alterations, the investigated horses were allocated into two main groups: ER-group (n = 7) and myositis group (n = 33). Blood samples...
Source: Comparative Clinical Pathology - February 14, 2018 Category: Pathology Source Type: research

Quercetin, rutin, and their combinations modulate penile phosphodiesterase-5 ′, arginase, acetylcholinesterase, and angiotensin-I-converting enzyme activities: a comparative study
In conclusion, our fi ndings suggest that the combination of quercetin and rutin is more potent than their individual effect. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Effect of traumatic reticulitis and allied syndromes on blood constituents in buffaloes
This study was aimed to evaluate the effect of traumatic reticulitis and other affections on some blood variables in buffaloes. Female buffaloes (n = 62) were divided into eight groups: control, non-penetrating reticular foreign bodies (NPRF), traumatic reticulitis (TR), traumatic reticuloperitonitis (TRP), traumatic reticuloperitonitis with peri-reticular abscess (TRP + PA), traumatic reticuloperitonitis with vagus indigestion (TRP + VI), diaphragmatic hernia (DH), and traumatic pericarditis (TP). Results revealed a significant decrease in RBC count in cases of NPRF, TR, TRP, TRP + PA, and TP. Platelet count...
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Genetic characterization and phylogenetic analysis of hemotrophic mycoplasmas in camel ( Camelus dromedarius )
AbstractSo far, a number of hemotrophic mycoplasma (HM) species have been reported from different animals, mostly based on the 16S rDNA gene sequence analysis. Although previous studies have describedCandidatus Mycoplasma haemolamae from alpaca (Lama pacos), llama (Lama glama), and vicuna (Lama vicugna) in Camelidae, data on the causative agent(s) of hemotrophic mycoplasmosis or hemoplasmosis are insufficient in dromedary camel (Camelus dromedarius). The purpose of the present molecular study was to investigate hemotrophic mycoplasmosis in Iranian dromedary camel. Accordingly, a total number of 100 EDTA-anticoagulated bloo...
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Chronic immune thrombocytopenia. Egyptian experience
In this study, we reviewed the demographic features of thrombocytopenic Egyptian patients. We also analyzed the role of T cell in chronic immune cases. IL-12, IL-35, IL-17, and TGF- β were measured by ELISA. The median age at the time of diagnosis was 30 years and its range was 14–70. The median platelet count at the time of diagnosis was 15 × 109/L. Regarding treatment and follow-up, there was an indication for treatment in 96% of patients. Of the 150 ITP patients who were given first-line therapy (corticosteroid 1  mg/kg/day PO), there was a complete response (CR) in 40.3% while 5...
Source: Comparative Clinical Pathology - February 13, 2018 Category: Pathology Source Type: research

Assessment of two abdominal mass closure methods using fishing line in rabbit model: clinical and pathological study
This study has demonstrated that fishing line, as suture material, is suitable for mass abdom inal closure in rabbit model. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 9, 2018 Category: Pathology Source Type: research

Effects of atorvastatin on myocardial oxidative and nitrosative stress in diabetic rats
AbstractFree radicals play a pivotal role in many pathophysiological states, such as myopathies. Atorvastatin is a known cholesterol-lowering agent with many pleiotropic actions including antioxidant properties. However, the impact of atorvastatin on myocardial oxidative stress is not well known. The aim of this study was to evaluate the role of atorvastatin in improving diabetes-induced oxidative stress in the myocardium. Male Wistar rats weighing 20 –25 g were randomly divided into four groups as normal, normal-treated, diabetes, and diabetes-treated. Induction of diabetes was performed by a single dose of str...
Source: Comparative Clinical Pathology - February 8, 2018 Category: Pathology Source Type: research

The study on rate of morbidity of fungal infections exiting in educational hospitals: Iran
AbstractWhereas hospitals have been assumed as the sites for spreading infection at level of world health, therefore, the current descriptive study has been conducted on 248 inpatients and outpatients who were suspected to fungal infection or disease. Samples were collected from several wards along with radiographic and endoscopic analyses in three hospitals, including urine, blood, skin, nail, wound (burning, surgery, eye and injury), lung, phlegm, ascites, bronchoaveolar lavage, (cerebrospinal, abdominal, and knee) fluids, peritoneum, fistula, ear tests and biopsy and sent to reference mycological center. The fungal spec...
Source: Comparative Clinical Pathology - February 7, 2018 Category: Pathology Source Type: research

Investigation of histopathological changes caused by adult stage of Linguatula serrata in dog
AbstractLinguatula serrata, known as tongue worm, is an anomalous ubiquitous parasite. This parasite inhabits the canine respiratory system as its final host. The discharged eggs cause to contamination of many plants that are consumed by feeder animals including human causing visceral and nasopharyngeal linguatulosis that is known as “Marrara syndrome.” This research was conducted for the first time to investigate the distribution and pathological findings ofL. serrata adult in nasal cavity of road-killed dogs in Uremia, Iran. For doing so, 52 dogs were inspected. During the necropsy;L. serrata adult was seen i...
Source: Comparative Clinical Pathology - February 4, 2018 Category: Pathology Source Type: research

Prevalence and antifungal susceptibility of mycotic agents isolated from skin lesions of horses in Enugu State, Nigeria
This study has provided baseline information which includes sex and seasonal distributions of the fungal agents associated with skin lesions in trade horses in Obollo-Afor area of Enugu State. Antifungal susceptibility results shows that voriconazole and nystatin are the drugs of choice in the treatment of dermatomycoses of horses in Nigeria. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 3, 2018 Category: Pathology Source Type: research

A novel herbal beverage from Moringa oleifera , Dennettia tripetala , and Citrus sinensis abates Bonny light crude oil-induced oxidative injury in rat brain and liver
AbstractA novel herbal beverage (HB) was formulated from blends ofMoringa oleifera,Dennettia tripetala, andCitrus sinensis and investigated for its antioxidative protective effect against Bonny light crude oil (BLCO)-induced oxidative injury in rat brain and hepatic tissues. Male albino rats were orally exposed to BLCO (800  mg/kg bodyweight) for 7 days, then treated with HB (200 and 400 mg/kg bodyweight) for another 7 days. Reduced glutathione (GSH) and malondialdehyde (MDA) levels were depleted and elevated respectively in both tissues on exposure to BLCO, which was (p 
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Potential of the ImageJ software in the differentiation of various types of canine lymphadenopathies with fine needle aspiration cytology (FNAC)
This study was designed to evaluate the potential of the ImageJ software in the differentiation of various types of lymphadenopathies with fine needle aspiration cytology (FNAC). For this purpose, the samples of enlarged mandibular lymph node were selected from 46 dogs suspected of lymphadenopathy and 10 healthy dogs as a control. The target tissues were obtained from the animals by fine needle aspiration and lymph node removed by surgery. The samples were then prepared for cytological and histological study. Finally, the ImageJ software was used to evaluate the quantitative nuclear morphometric parameters. The data obtain...
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Effect of powdered seed of Nigella sativa administration on sub-chronic and chronic lead acetate induced hemato-biochemical and histopathological changes in Sprague Dawley rats
This study hypothesized that theN. sativa (NS) administration will ameliorate the deleterious effects of chronic PbAC toxicity in rats. A total of 75 Sprague Dawley rats were divided into three groups of 25 rats, and each group was further sub-divided into five groups of five rats each. Group 1 rats (negative control) were given distilled water, group 2 (positive control; PC) were given 10  mg/kg of lead acetate (PbAC) daily, and groups 3 (T1), 4 (T2), and 5 (T3) were each given 10 mg/kg of PbAC followed by graded concentrations of powdered seeds of NS; 100, 150, and 200 mg/kg, respectively. Five rats in eac...
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Bracken fern toxicity and its associated clinicopathological effects in humans and animals: a review
In conclusion, special attention should be given to this plant by toxicologists, botanists, veterinarians, toxicopathologists, oncologists and public health specialists. The human populace, farmers inclusive, should be educated about the public health significance of this plant. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 2, 2018 Category: Pathology Source Type: research

Detecting endomysial and tissue transglutaminase antibodies in patients with giardiasis
AbstractCeliac disease is a genetic disease diagnosed to be associated with chronic intestinal inflammation. Endomysial and tissue transglutaminase antibodies are the best serum markers for celiac disease (CD) diagnosis. This research aimed to determine the levels of endomysial and tissue transglutaminase antibodies in patients with giardiasis. Forty cases of giardiasis were selected among the referees to Milad Hospital in Tehran, as well as a children ’s hospital and some health centers in Karaj. Euro-immune IgA immunofluorescence and ELISA were utilized to measure their titers of endomysial and tissue transglutamin...
Source: Comparative Clinical Pathology - January 30, 2018 Category: Pathology Source Type: research

Growth performances, survival rate, and biochemical parameters of Nile tilapia ( Oreochromis niloticus ) reared in water treated with probiotic
AbstractThe growth performances, survival rate, and biochemical parameters of Nile tilapia (Oreochromis niloticus) reared in water treated with probiotic were investigated. Samples of 225 Nile tilapia (5.8  ± 0.10 g) were reared for 90 days in three different treatments with three replications as the control group (no probiotic), and two test groups with probiotics consisting of yeast (Saccharomyces cerevisiae) (5.6  × 108 cfu g−1) andBacillus spp. (B. subtilis,B. megaterium, andB. licheniformis) (1  × 106 cfu g−1) added ...
Source: Comparative Clinical Pathology - January 29, 2018 Category: Pathology Source Type: research

Is vitamin D supplement accompanied with narrow band UVB effective for treatment of vitiligo?
AbstractVitiligo is an acquired autoimmune depigmenting disorder that is the result of white macules formation in the skin and mucous membranes, caused by the loss of melanocytes. It has affected 0.4 to 2.0% of world ’s population. It has been hypothesized that Vitamin D is important for skin pigmentation, but the correlation between vitamin D and vitiligo treatment response needs to be evaluated. To evaluate vitamin D supplementation effect on the vitiligo area severity index in patients treated with narrow b and UVB (NB UVB). This clinical pilot randomized study was carried out on 48 vitiligo patients who admitted ...
Source: Comparative Clinical Pathology - January 29, 2018 Category: Pathology Source Type: research

Dramatic shortening of the diagnosis of multidrug-resistant tuberculosis by the detection of rifampicin resistance using a genotypic method: GeneXpert MTB/RIF assay
AbstractIn spite of all the evolutions in medicine, tuberculosis (TB) continues to be a terrifying contagious disease. In addition, the rise of multidrug-resistant TB (MDR-TB) is considered as a thorny problem that has greatly hindered the efforts of TB control. The classical drug susceptibility testing (DST) is the reference technique, but it takes many weeks for the identification of TB and MDR-TB. On the other hand, GeneXpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) has dramatically shortened the identification of both MTB and RIF resistance from weeks to only 2  h leading to a great breakthrough in the mo...
Source: Comparative Clinical Pathology - January 27, 2018 Category: Pathology Source Type: research

Protective potential of Tetrapleura tetraptera against trona ( kaun )-induced hepatic injury in rat models
AbstractThe antioxidant protective effect of the fruits and peels ofTetrapleura tetraptera were investigated in trona (kaun)-induced oxidative injuries in male albino rats. The peels and fruits ofT. tetraptera were air-dried and extracted with ethanol. The concentrated extracts were subjected to FTIR spectroscopy analysis to identify their chemical functional groups. Five groups of six rats each were pretreated with trona (500  mg/kg bw) for 7 days, while a sixth group was administered water only which served as normal control. Four of the pretreated groups were subjected to posttreatment withT. tetraptera extrac...
Source: Comparative Clinical Pathology - January 25, 2018 Category: Pathology Source Type: research

Mesenteric cystic lymphangioma: a case report
AbstractIn adult, cystic lymphangioma of mesentery is an extremely rare manifestation of an intraperitoneal tumor. The clinical presentations of it are variable and occasionally area incidental finding. Herein, we describe a mesenteric cystic lymphangioma in a 39-year-old Iranian woman. The patient was presented with abdominal pain in the periumbilical area from 6  h earlier in the morning. Histopathological review of the excised mass revealed large numbers of cystic lymphatic channels lined by flattened endothelial cells and had smooth muscle bundles in their walls. The patient had an uneventful postoperative period....
Source: Comparative Clinical Pathology - January 25, 2018 Category: Pathology Source Type: research