Effects of asiatic acid, an active constituent in Centella asiatica (L.): restorative perspectives of streptozotocin-nicotinamide induced changes on lipid profile and lipid metabolic enzymes in diabetic rats
AbstractThe present study was aimed to evaluate the restorative efficacy of asiatic acid (AA), an active constituent inCentella asiatica (L.) in streptozotocin (STZ)-nicotinamide (NAD)-induced diabetic rats. Male Wistar albino rats were made diabetic with STZ-NAD administration and assessed the effect of AA (20, 30, and 40  mg/kg body weight per day, for 45 days) on body weight, glucose, insulin, insulin resistance, lipid metabolic parameters in plasma and tissue (liver and kidney), and histopathology of liver in control and experimental diabetic rats. A significant (p 
Source: Comparative Clinical Pathology - May 23, 2019 Category: Pathology Source Type: research

Publishing quandaries for scientists from developing countries
AbstractResearchers ought to publish their research articles in a scientific journal to get their work recognized. Publication of scientific research output is crucial for the dissemination of scientific information. But, they are confronted with variety of challenges in publishing their findings, even more when they are researchers from developing countries. I therefore in this paper wish to draw attention to the challenges faced by scientist from developing countries to document their work keeping my focus on one of the major criteria set by journals, i.e., inclusion of molecular tests and/or novelty which are in particu...
Source: Comparative Clinical Pathology - May 22, 2019 Category: Pathology Source Type: research

Effect of radio frequency electromagnetic field from mobile phones ’ base station antennas on maturation of rat erythrocytes
AbstractThe biological effects of radiation emitted by mobile telephone base station antennas are still controversial. The present study was designed to evaluate the effects of radio frequency electromagnetic field frequency range of 1200 –1400 MHz, from a mobile phone base station on erythropoietic cells in the rat bone marrow. Out of a total of 48 rats, 24 were exposed for 2 h each day for 7, 14, 30, and 60 days to electromagnetic radiation at specific absorption rate (SAR) levels 1.165 W/kg. Electromagnetic field–exposed r ats were compared with sham-exposed control rats. The animals of t...
Source: Comparative Clinical Pathology - May 20, 2019 Category: Pathology Source Type: research

Clinical and laboratory investigations of poor performing anemic Egyptian horses infested with Theileria equi
This study aimed to evaluate hematology, biochemical constituents, acid-base balance, and blood gases in poor-performing anemic working horses. Thirty Egyptian Balady horses divided into two groups (15 healthy horses as a control group and 15 poor-performing anemic workin g horses). Blood samples were taken from each animal and divided into three portions, the first portion collected on EDTA tubes for hematology examination, the second portion collected on Heparin and sent immediately for blood gases and acid-base estimation, and the last portion collected on plain t ubes for serum separation. Giemsa-stained blood films we...
Source: Comparative Clinical Pathology - May 19, 2019 Category: Pathology Source Type: research

Biochemical changes in selected tissues of normal albino rats following treatment with 1,3-bis [(furan-2-yl) methylene] urea
AbstractThis research investigated the effects of 1,3-bis(furan-2-yl)methylene) urea (BFMU), a synthesized imine compound with potential biological activities, on selected biochemical parameters of albino rats. The compound at varied concentrations (12.5, 25, and 50  mg/kg body weight) was administered intraperitoneally to albino rats for 28 days. At the end of the experiment, activities of enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) were assayed. Also, activities o f antioxidants including catalase (CAT), superoxide di...
Source: Comparative Clinical Pathology - May 19, 2019 Category: Pathology Source Type: research

Activity of Adansonia digitata on biochemical and hematological changes in established Plasmodium berghei -infected mice
This study evaluated the efficacy ofAdansonia digitata stem bark extract on hematological and renal changes in establishedPlasmodium berghei infection in mice. Albino wistar mice were used in the study. The mice were transfected intraperitonially with an inoculum size of l  × 107 of chloroquine susceptible strain ofPlasmodium berghei-infected erythrocytes. Percentage chemosupression and packed cell volume (PCV) were determined. The antiplasmodium effects of the extracts were investigated by measuring the hemoglobin (Hb) content, white blood cell (WBC) count, and cell differentials. Severity of malaria w...
Source: Comparative Clinical Pathology - May 17, 2019 Category: Pathology Source Type: research

Serum biochemical profiling in different varieties of Japanese quail, Coturnix coturnix japonica (Temminck and Schlegel, 1849)
AbstractA good quantum of reports is in record on serum biochemical analysis of Japanese quail. But the serum biochemical parameter of different varieties of healthy Japanese quail is poorly understood, and reference values are sparsely available. The investigation focuses to assess serum biochemical profiles of three different varieties of Japanese quail, i.e. brown-feathered, grey-feathered, and white-feathered at different age groups with sexual dimorphism. The five parameters taken for the analyses include glucose, cholesterol, total protein, albumin, and globulin. Two millilitre of blood was collected from the ulnar v...
Source: Comparative Clinical Pathology - May 17, 2019 Category: Pathology Source Type: research

Brilliant blue G protects against brain and liver tissue damage during systemic endotoxemia in rats treated with lipopolysaccharide
In this study, the effect of brilliant blue G on serum oxidative stress and brain and liver tissue damage was studied in rats with lipopolysaccharide (LPS)-induced systemic inflammation. Rats were treated with intraperitoneal (i.p.) injection ofEscherichia coli LPS (300  μg/kg) alone or together with brilliant blue G at 5 or 10 mg/kg and euthanized 4 h thereafter. Serum malondialdehyde, nitric oxide, paraoxonase 1 (PON-1) activity, cholinesterase activity, and glucose were determined. In addition, brain and liver histopathology, and caspase-3 and glial fibrilla ry acidic protein (GFAP) immunostaining were...
Source: Comparative Clinical Pathology - May 17, 2019 Category: Pathology Source Type: research

Effects of aqueous leaf extracts of Loranthus micranthus Linn. on hematological profile of albino rats infected with Trypanosoma brucei brucei
AbstractsAfrican trypanosomiasis has continued to threaten human health and economical development (Kuzoe Acta Trop 54:153 –162,1993; WHO J Tsetse Tryp Info Quart 3:4 –9,2000). It is caused by parasitic protozoa of the genusTrypanosoma (Adeiza et al. J Med Plant Res 4(17):1770 –1777,2010), and the distribution corresponds with that of tsetse flies. Effects of aqueous leaf extracts ofLoranthus micranthus on hematological parameters of albino rats infected withTrypanosoma brucei brucei were investigated for 28  days using standard methods. The HB, PCV, RBC, and its indices (MCH, MCV, and MCHC) of the i...
Source: Comparative Clinical Pathology - May 13, 2019 Category: Pathology Source Type: research

Editorial
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 13, 2019 Category: Pathology Source Type: research

Cytotoxicity, antioxidant, anti-inflammatory activity, and GC-MS analysis of Egyptian propolis
AbstractNatural products have a great contribution to folk medicine because it is rich in various and unique compounds. Propolis is considered one of the most important natural products due to their different medical applications. In this work, the antioxidant, anti-inflammatory, and cytotoxicity of propolis to cancer cell lines were investigated which was obtained from Bee Research Department, Plant Protection Research Institute and Agricultural Research Center, Egypt. The antioxidant activity was evaluated by DPPH assay. The propolis concentration of 1280  μg/ml achieved the highest value (91.42%) of the antioxid...
Source: Comparative Clinical Pathology - May 10, 2019 Category: Pathology Source Type: research

Clinico-pathological responses in reproductive system and its associated lymph nodes of bucks challenged with Corynebacterium pseudotuberculosis and its mycolic acid extract
In conclusion, this research revealed thatC. pseudotuberculosis has a negative effect on reproductive performances of bucks and affects the clinical responses and abscess formation. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

Haematological, serum biochemical and histopathological changes in hepatitis E virus seropositive pigs in Ibadan, Nigeria
This study evaluated the haematological, serum biochemical and histopathological changes in HEV seropositive pigs. A total of 120 pigs were sourced from the Bodija Municipal abattoir, Ibadan, Nigeria, for this study. The haematology and serum biochemistry of the sampled pigs seropositive to HEV antibodies using the ID Screen ® Hepatitis E Indirect Multi-species ELISA biwell kit were analysed. Liver and spleen samples were also taken for histopathological examination and lesion scoring. HEV seropositivity was reported in 57.5% (69/120) of the pigs and associated with a significant (p 
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

A study of clinical and pathological findings in an ovine experimental theileriosis
AbstractThe malignant ovine theileriosis due toTheileria lestoquardi is highly pathogenic in sheep. During an experimental study, clinical and pathological findings were investigated in sheep infected withT. lestoquardi. The common clinical signs were high fever, emaciation, enlarged lymph nodes, anemia, icterus, nasal discharge, cough, severe pneumonia, circling, opisthotonus, recumbency, and death. Schizonts ofTheileria were detected in impression smears of the lymph nodes, spleen, and liver. Gross lesions include pale mucous membranes, enlarged lymph nodes, splenomegaly, and hepatomegaly with gall bladder distention. Pe...
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

Cutaneous horn ( cornu cutaneum ) in a Pakistani goat
In this report, this lesion was observed on the apex of the ear in a female Pakistani goat. The size of the cutaneous horn was 12  cm length. It was removed under local anesthesia and fixed in 10% neutral buffered formalin. Histopathologic examination showed well-differentiated keratinocytes without atypical keratinocytes. Thick dermal collagenous bundles were observed in the horn base. Mononuclear cell inflammation was obser ved around the vessels of the dermis in the base of lesion. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

Correction to: Evaluation of leukocyte esterase test strips for rapid diagnosis of subclinical mastitis in dairy cows
The affiliation of Abolfazl Hajibemani in the original published version of this article was incorrect. Instead of Tabriz University, it should have been University of Tabriz. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

Effects of medicinal plant powder as feed additives on growth performance, carcass characteristics, and immune response of Coturnix japonica against avian influenza and Newcastle disease vaccine virus
AbstractThe purpose of this study was to evaluate the potential effect of different levels ofGracilaria corticata (Gracilaria),Sargassum cristaefolium (Sargassum),Rhus coriaria (sumac), andPunica granatum (pomegranate) peel powdered on growth performance, carcass characteristics, and immune responses of Japanese quails against avian influenza and Newcastle disease killed vaccine virus. A total of 360 one-day-old Japanese quails were randomly distributed into nine dietary treatments with 4 replicates (10 quail/rep). Control group (C) received the diet free of medicinal plants supplementation and the other eight treatments r...
Source: Comparative Clinical Pathology - May 8, 2019 Category: Pathology Source Type: research

Changes in the cardiac phosphotransfer network associated with oxidative stress in experimentally infected Brucella ovis mice
AbstractThe aim of this study was to evaluate whetherBrucella ovis infection altered the activities of cardiac enzymes belonging to the phosphotransfer network and cause an oxidative imbalance in mice, used as an experimental model. We used hearts from 48 mice in two groups (n = 24 each): uninfected (control group) and infected byB. ovis, collected on days 7, 15, 30, and 60 post-infection (PI). Cardiac cytosolic creatine kinase (CK) activity was higher in infected animals than in the control group on days 30 and 60 PI, while cardiac mitochondrial CK activity was lower at the same moments of infection. Cardiac...
Source: Comparative Clinical Pathology - May 8, 2019 Category: Pathology Source Type: research

Prevalence of Dirofilaria immitis and Dirofilaria repens in outdoor dogs in Tehran Province, Iran
AbstractDirofilaria immitis andDirofilaria repens are the most common species of filarial nematodes described in dogs, which are endemic in Iran. The diagnosis of canine dirofilariosis is usually based upon the microscopical detection and identification of circulating microfilariae together with ELISA detection of serum circulating heartworm antigens or antibodies. The identification of the parasite species using the traditional approaches sometimes can lead to misdiagnosis. As a zoonotic disease, it is important to know the prevalence ofDirofilaria in Tehran that is the most crowded province with many outdoor and stray do...
Source: Comparative Clinical Pathology - May 7, 2019 Category: Pathology Source Type: research

Comparative study on hemato-biochemical alterations and selected acute phase protein response in native sheep experimentally infected with bluetongue virus serotypes 10 and 24
AbstractBluetongue (BT) is an infectious, non-contagious, insect-transmitted viral disease of sheep, other domestic, and wild ruminants caused by bluetongue virus (BTV), a prototype species of the genusOrbivirus of the familyReoviridae. The study was designed to determine the hematological and biochemical alterations and acute phase protein response in BTV sero-negative sheep experimentally infected with bluetongue virus serotype-10 and 24. The BTV-infected group comprising of 18 animals, 6 each inoculated with 6  ml of clarified virus containing 1 × 106/ml TCID50 of BTV-10 or 24 by intradermal rou...
Source: Comparative Clinical Pathology - May 7, 2019 Category: Pathology Source Type: research

The effect of Descurainia sophia oil on methamphetamine-induced cell cytotoxicity and cell death in PC12
In this study, protective effects ofDescurainia sophia oil were followed up in methamphetamine-induced cell cytotoxicity in a neuron-like PC12 cell line. The viability, proliferation, and cytotoxicity of the cells were assessed by Trypan blue, MTT test, and lactate dehydrogenase (LDH) assay, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was performed to detect DNA fragmentation and apoptosis. Nitric oxide (NO) level was detected by Griese method. Interleukin-1 β (IL-1β), IL-6, interferon gamma (INF-γ), and tumor necrosis factor alpha (TNF-α) pro-inflammatory c...
Source: Comparative Clinical Pathology - May 7, 2019 Category: Pathology Source Type: research

Aging of bovine spinal cord: an alteration of myelinated nerve fibers in the white matter
AbstractTo gain more knowledge of age-related changes in the spinal cord of the bovine species, the structure of 15 clinically normal aged cattle (Holstein-Friesian cows 8 –13 years of age) was histopathologically examined. In 12 (80%) of the 15 animals, the common histopathological features identified were alteration of a small number of myelinated nerve fibers in the white matter, with less than 4 nerve fibers affected per cross-sectional area of the spinal cord. Alteration of nerve fibers was characterized by dilation of myelin sheaths with loss of axons or macrophage infiltration, resembling features of Wall...
Source: Comparative Clinical Pathology - May 5, 2019 Category: Pathology Source Type: research

Anti-plasmodial, Anti-inflammatory, anti-nociceptive and safety profile of Maytenus senegalensis root bark extract on hepato-renal integrity in experimental animals
AbstractMaytenus senegalensis is a plant with several medicinal claims in folk medicine. Methanol root extract of the plant was evaluated for antiplasmodial, anti-inflammatory, anti-nociceptive, and safety profile. Antiplasmodial, anti-inflammatory (egg albumin induced paw edema), and analgesic (hot plate test) experiments were set up, each consisting of four (4) groups (A-D) of five mice each. Groups A –C of each experiment were treated with 2.0 ml/kg bw normal saline, 400 and 800 mg/kg bw of the extract according to the procedures. Chloroquine, acetyl salicylic, and aspirin respectively were used as the r...
Source: Comparative Clinical Pathology - May 1, 2019 Category: Pathology Source Type: research

Disseminated histoplasmosis in a Brazilian domestic cat: early diagnosis and successful treatment
AbstractHistoplasmosis is a worldwide distributed opportunistic mycosis caused by the dimorphic fungusHistoplasma capsulatum. It is reported in cats with non-specific clinical signs. The aim of this case report is to describe the first case of disseminated histoplasmosis in a cat in the city of Santa Maria, Rio Grande do Sul, Brazil. In veterinary consultation, it was reported that the animal had a history of vomiting, hematochezia, and weight loss for 3  months. At physical exam, an abdominal lymph adenomegaly, an intestine diffusely enlarged, and a poor body condition were observed. Ultrasound-guided fine-needle asp...
Source: Comparative Clinical Pathology - April 28, 2019 Category: Pathology Source Type: research

Ameliorative effects of alpha-lipoic acid and imidocarb dipropionate on clinico-haematological changes induced by experimental Babesia canis vogeli infection in dogs
In conclusion, co-administration of imidocarb with ALA acted synergistically and also ameliorated the clinico-haematological changes in experimental canine babesiosis, a nd the agent may be a promising and potent drug in the management of canine babesiosis. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 26, 2019 Category: Pathology Source Type: research

Assessing the possible causes of hemolytic anemia associated with lumpy skin disease naturally infected buffaloes
This study concluded that buffaloes show limited susceptibility to LSD, although the presence of hypophos phatemia could exaggerate hemolytic anemia under an oxidant-anti-oxidant imbalance and increased pro-inflammatory cytokines. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 26, 2019 Category: Pathology Source Type: research

Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles
The objective of this study was to evaluate and quantify the fat storage in the liver of lesser guitarfishZapteryx brevirostris submitted to different periods of restriction and refeeding. In a water recirculation system, 21 animals were divided into three groups: control (n=7), fed every day; W1 (n=7), with one week of restriction and two of refeeding and W2 (n=7), with two weeks of restriction and two of refeeding. After 76 days, the animals were euthanized and liver samples were collected, paraffin embedded, stained with PAS and evaluated with stereological procedures and histopatological changes were recorded. The area...
Source: Comparative Clinical Pathology - April 21, 2019 Category: Pathology Source Type: research

Systemic lipofuscinosis associated with a lesion of autophagic vacuolar myopathy in the diaphragmatic muscle of a cow
AbstractA 7-year-old Holstein-Friesian dairy cow that was culled because of traumatic motor dysfunction due to accidental falling down showed a variety of gross postmortem findings, such as fracture of the caudal vertebra, massive hemorrhage in the pelvic cavity, edematous swelling of the right sciatic nerve, and brown discoloration of the masseter muscle, tongue, cardiac muscle, diaphragmatic muscle, liver, adrenal cortex, and intestinal wall. Histopathologically, the discolored and some other internal organs/tissues exhibited varying degrees of intracellular deposition of lipofuscin granules. A small number of eosinophil...
Source: Comparative Clinical Pathology - April 15, 2019 Category: Pathology Source Type: research

Gastroprotective effects of mumie on aspirin-induced gastric lesions in rats
AbstractThe present research was carried out to investigate the influence of mumie aqueous extract on aspirin-induced gastric lesions in rats. Thirty-eight male animals were grouped as the following: Normal control received 1  ml normal saline; positive control received 1 ml normal saline; standard control received omeprazole (20 mg/kg); treatment received mumie (600 mg/kg). After 1 h all the rats (except the normal control group) were given 500 mg/kg aspirin to induce lesions. Four hours after lesion induction, th e gastric injuries were assessed through the evaluation of the macroscopic appe...
Source: Comparative Clinical Pathology - April 14, 2019 Category: Pathology Source Type: research

Diagnosis and treatment of canine leptospirosis due to serovar Bataviae —a case report
AbstractInfection ofLeptospira interrogans var. Bataviae in a properly vaccinated German shepherd dog and its successful clinical management are described. The dog was presented with a history of anorexia, vomiting, oliguria, melena, lethargy, and weakness. The animal had a proper history of yearly vaccination with the multicomponent vaccine. Hematology revealed leucocytosis, thrombocytopenia, granulocytosis, and anemia. Serum biochemical analysis showed elevated BUN, creatinine, ALP, total bilirubin, hypoalbuminemia, hyperglobulinemia, and lower A:G ratio. Based upon the clinical signs and blood-biochemical changes, the c...
Source: Comparative Clinical Pathology - April 12, 2019 Category: Pathology Source Type: research

The beneficial effect of equine chorionic gonadotropin hormone (eCG) on the in vitro co-culture of bovine spermatogonial stem cell with Sertoli cells
AbstractSpermatogenesis is a complex developmental process that originates from spermatogonia stem cell. This process consists of sequential, highly organized steps of cell proliferation and differentiation resulting in the generation of functional spermatozoa. Many growth factors, hormones, and cell interactions of germ cells with Sertoli cells regulate these processes, and the failure of any of the processes can lead to male infertility The aim of the present study was to determine the effects of equine chorionic gonadotropin on spermatogonial stem cell colony formation and differentiation after in vitro co-culture with ...
Source: Comparative Clinical Pathology - April 9, 2019 Category: Pathology Source Type: research

Oxidative stress and stroke: a review of upstream and downstream antioxidant therapeutic options
AbstractThe stroke is a cerebrovascular disorder and is the cause of a second mortality and long-lasting harm worldwide. It is classified into two types: hemorrhagic and ischemic stroke. Globally, 13% of cases of stroke are hemorrhagic, while the remaining 87% are ischemic. In this paper, we discuss the consideration of factors which possibly affect the effectiveness of antioxidant protection under oxygen deprivation, as well as oxidative stress. Such aspects as compartmentalization of reactive oxygen species (ROS)/reactive nitrogen species (RNS) formation and antioxidant localization, synthesis and transport of antioxidan...
Source: Comparative Clinical Pathology - April 8, 2019 Category: Pathology Source Type: research

Biochemical changes in Curimbat á subjected to transport stress and exposed to an agricultural fair
AbstractFish known as Curimbat á (Prochilodus lineatus) suffered a reduction in natural stocks due to predatory fishing and environmental deterioration; these are among the reasons for increased interest in the inclusion of this species in agricultural fairs. The aim of this study was to evaluate whether transport and exposure to an agricultural fair alters plasma biochemical parameters in curimbat ás. Blood samples were collected at 0 h (before transport), 10 h (end of transport), and 72 h (end of exposure to agricultural fair). Plasma cortisol levels increased after 72-h exposure to agricultu...
Source: Comparative Clinical Pathology - April 4, 2019 Category: Pathology Source Type: research

Investigations on the antimicrobial and wound healing activity of Ficus thonningii Blume extracts
In conclusion, 7.5% ointment prepar ation of ethyl acetateF. thonningii leaf extract could be considered as a potent herbal drug for treating wound infections. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 1, 2019 Category: Pathology Source Type: research

Metabolic status and ultrasound traits of reproductive tract of estrus dairy cows in early postpartum period
This study indicated that the correlation between ultrasound traits of uterus and metabolite hormones was not significant at the clean test in estrus dairy cows. Moreover, there was no relationship between serum leve l of IGF-1, NEFA, and BHBA with either dominant follicle diameter or existence of corpus luteum (CL). Significant association between ultrasound properties of the uterus and ovaries and metabolic hormone (IGF-1, NEFA, and BHBA) concentrations did not reveal in estrus dairy cows at day 28 ± 3 po stpartum. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 1, 2019 Category: Pathology Source Type: research

Antiulcer activity of aqueous extract of leaves of Mentha piperita in Wistar rats
In conclusion, it seems that the aqueous extract ofM. piperita can prevent gastroduodenal ulcers in rats without any side effect. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 16, 2019 Category: Pathology Source Type: research

Effect of two different habitat on haematological parameters of fishes of Odisha
AbstractThe aim of this study was to assess the influence of different water quality of Kausalyaganga pond of Bhubaneswar and Kalupada ghat, the northern sector of Chilika lagoon of Khordha district, Odisha on the haematological and morphometry of erythrocytes ofHeteropneustes fossilis,Clarias batrachus,Channa punctatus,Channa striatus andAnabas testudineus. In both sampling sites, water physical-chemical-parameters of the water were measured. Our results showed statistical difference in pH, alkalinity, salinity and dissolved oxygen between the two sites. Haematological parameters like haemoglobin (Hb), red blood cell coun...
Source: Comparative Clinical Pathology - March 16, 2019 Category: Pathology Source Type: research

Investigation of eucalyptus essence 1% and its mixture with licorice gel 10% on the infected third-degree burn wound in rat model
AbstractMany people have irreversible defects and to be death due to burn wounds every year. The current study was conducted to assess the effects of eucalyptus essence (EE) and its mixture with licorice in burn wound healing in rats. One hundred fifty rats were divided to five equal groups. Twenty-four hours after induction of third-degree burn wound, inoculation of 108 colony-forming units ofPseudomonas aeruginosa (PA 103) into the burnt area was performed in form of subcutaneous. Group 1 was given no treatment, and group 2 received gel base. Group 3 (positive control) was treated with silver sulfadiazine (SSD). Groups 4...
Source: Comparative Clinical Pathology - March 16, 2019 Category: Pathology Source Type: research

Griffonia simplicifolia (DC.) Baill. attenuates gentamicin and cisplatin-induced nephrotoxicty in rats
This study was designed to evaluate the nephroprotective activity of 50% hydroethanolic leaf extract ofGriffonia simplicifolia (DC.) Benth in drug-induced nephrotoxicity in Sprague –Dawley rats. Nephrotoxicity was induced in experimental animals by administering gentamicin and cisplatin after pre-treatment with hydroethanolic extract ofG. simplicifolia (GSE). GSE at 100 and 250  mg/kg were administered for 7 and 10 days by oral gavage in the gentamicin and cisplatin models, respectively. Silymarin (120 mg/kg) was given as the standard nephroprotective drug. Nephroprotective effect was studied by assayi...
Source: Comparative Clinical Pathology - March 13, 2019 Category: Pathology Source Type: research

Surgical treatment of large intestinal intussusception in a Rottweiler puppy
AbstractA 3-month Rottweiler female dog approximately weighing 5  kg was referred to veterinary clinic with history of defecation ceasing symptoms for about 48 h. In the clinical examinations, anorexia, lethargy, and frequent vomiting were observed. The blood work showed an increase in neutrophils. The mucosa was dark color and the animal CRT was increased. Acc ording to the clinical symptoms and blood work findings, a simple radiograph was considered to be taken from the abdominal cavity and the layering of gas-filled intestine was seen, which decided to take the radiograph with the contrast media. In the radiol...
Source: Comparative Clinical Pathology - March 12, 2019 Category: Pathology Source Type: research

Serum protein electrophoretic profile in diarrheic neonatal calves
AbstractThe electrophoretic profile of clinically healthy newborn calves and calves with neonatal diarrhea was compared. Blood samples were collected at 3 and 10  days of age from 11 calves that remained clinically healthy throughout the study period and 14 calves with diarrhea. A significant reduction in the gamma globulins was observed in the diarrheic calves that can be attributed to the transfer of serum immunoglobulins, to the intestinal lumen to count eract the infection. Moreover, a significant increase in the albumin was observed in this group between the sampling days that is mainly attributed to the relative...
Source: Comparative Clinical Pathology - March 11, 2019 Category: Pathology Source Type: research

Biochemical reference intervals for the Italian Heavy Draft horse
AbstractEstablished breed-specific reference intervals are important for monitoring the health status of horses. Serum biochemical breed-specific reference intervals have been evaluated in horses and other animal breeds, but there are no studies for the Italian Heavy Draft horse. The aim of this study was to determine biochemical reference intervals for the Italian Heavy Draft horse living in central Italy. Moreover, the comparison between different physiological status (pregnancy and no pregnancy) and age (foals and adults) has been executed. Inclusion criteria were established before blood collection. Blood samples were ...
Source: Comparative Clinical Pathology - March 8, 2019 Category: Pathology Source Type: research

Comparison of transduction efficiency among various cell types by a lentivector containing CMV promoter
In this study, the third-passage isolated MSCs from four different species, including ovine and goat cardiac fibroblasts, HEK293T, and L929 cell line were transduced by concentrated virus particles. Then, transduction percentage was detected by flow cytometry. The findings showed different transduction efficiencies for various cells of one species or other species, indicating that differentiated and undifferentiated cells had different transduction percentages. Different degrees of transduction observed in differentiated and undifferentiated cells might be the result of various lentiviral receptors on the plasma membrane, ...
Source: Comparative Clinical Pathology - March 3, 2019 Category: Pathology Source Type: research

The association between metabolic profile indices, clinical parameters, and ultrasound measurement of backfat thickness during the periparturient period of dairy cows
This study aimed to evaluate clinical and biochemical changes in dairy cows during periparturient period. It was carried out on 38 multiparous Holstein/Fersian crossbreed dairy cows 3 –11 years old. Using ultrasonography for measurement backfat thickness (BFT) for detection of the energy deficit. BFT on dairy cattle is used to monitor changes in subcutaneous fat thickness which represent changes in energy stores. On the basis of clinical examination and assessment of BHBA conc entration in blood, cows were classified to four groups, control healthy cows (C = 16), subclinical ketosis (SCK =&t...
Source: Comparative Clinical Pathology - February 28, 2019 Category: Pathology Source Type: research

Clinical observation, acute phase protein levels, and histopathological changes of mammary gland in experimentally infected goats with Staphylococcus aureus
AbstractStaphylococcus aureus (S. aureus) is a major cause of mastitis in small ruminants, and studies on the potential application of acute phase proteins (APPs) in disease biomarkers and understanding the disease mechanism in mastitis affecting small ruminants are limited. Thus, the objective of this study was to determine clinical, histopathological changes and serum concentrations of serum amyloid A (SAA) and haptoglobin (Hp) associated with experimentally induced mastitis usingS. aureus. Left mammary quarter in the does was challenged with sterile Ringers buffered salt solution as group 1 (control animals;n =&t...
Source: Comparative Clinical Pathology - February 27, 2019 Category: Pathology Source Type: research

Impact of environmental changes on plasma biochemistry and hematological parameters of Himalayan snow trout, Schizothorax plagiostomus
AbstractThe hematological and plasma biochemical parameter study is recognized as a valuable tool for evaluating health status, physiological and pathological conditions of fish. In the present study, blood sampling was carried out with the aim to evaluate plasma biochemistry and hematological parameters of Himalayan snow trout,Schizothorax plagiostomus, that are altered due to variation in environmental conditions. The fluctuations in hematological parameters such as hemoglobin (Hb), red blood cell (RBC) count, packed cell volume (PCV), white blood cell (WBC) count, mean corpuscular hemoglobin (MCH), mean corpuscular hemo...
Source: Comparative Clinical Pathology - February 26, 2019 Category: Pathology Source Type: research

In vitro and in vivo studies on the alpha-glucosidase inhibitory effects of the leaf extract of Carica papaya Linn
AbstractThe search for novel alpha-glucosidase inhibitory agents is currently an important scientific endeavour to the biopharmaceutical companies due to their potentials to reduce postprandial hyperglycaemia in type 2 diabetic patients. The present research attempted to investigate the alpha-glucosidase inhibitory effects of the leaf extract ofCarica papaya. Aqueous, methanol, ethyl acetate and n-hexane extracts ofC. papaya leaf were subjected to in vitro alpha-glucosidase inhibitory studies, and methanol extract was the most potent (IC50 = 171.83 ± 26.89 μg/mL). Consequently, th...
Source: Comparative Clinical Pathology - February 26, 2019 Category: Pathology Source Type: research

The effect of tramadol and meloxicam, alone and in combination on oxidative stress status in dogs
AbstractManagement of pain by different therapeutic agents has always been one of the most important components in clinical veterinary care. Concerns about various side effects of analgesics drugs have lead the pain relief to combination drug therapy. The present study was performed to evaluate the effect of combined tramadol and meloxicam on oxidative stress status in dogs. Twenty clinically healthy dogs were randomly divided into four groups and administered with placebo, tramadol, meloxicam, and combined meloxicam with tramadol for 10  days. The antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidas...
Source: Comparative Clinical Pathology - February 23, 2019 Category: Pathology Source Type: research

Ultrastructural changes on fertile and infertile hydatid cysts induced by conventional and solid lipid nanoparticles of albendazole and albendazole sulfoxide
AbstractCystic echinococcosis (CE), a serious public health problem worldwide, is caused by the larval stage ofEchinococcus granulosus (E. granulosus). Despite recent progress in the CE medical treatment, this subject still remains as a priority of research. Albendazole (ABZ) is a drug of choice for the treatment of hydatidosis. Its use as an antiparasitic agent with the most efficacy is limited by the lack of water solubility. Therefore, the aim of the present study was to compare in vitro efficacy of free and solid lipid nanoparticles (SLNs) of ABZ and albendazole sulfoxide (ABZSO) against fertile and infertile hydatid c...
Source: Comparative Clinical Pathology - February 21, 2019 Category: Pathology Source Type: research

Hematological and serum biochemical parameters of five freshwater snow trout fish species from river Jhelum of Kashmir Himalaya, India
AbstractFish blood is a pathophysiological indicator of the whole body function and is thus considered as an important tool in diagnosing the structural and functional status of fish. However, the blood parameters vary between species to species and it mainly depends upon the favorable environmental conditions where the species live. The purpose of the current study is to evaluate the hematological and serum biochemical indices of fiveSchizothorax species:Schizothorax labiatus,S. plagiostomus, S. esocinus,S. curvifrons andS. niger in order to establish the resemblances and variations between theseSchizothorax species which...
Source: Comparative Clinical Pathology - February 19, 2019 Category: Pathology Source Type: research