Dose –response evaluation of cannabichromene on hepato-renal enzyme and non-enzyme biomarkers: the pharmacological controlled study
AbstractCannabichromene (CBC) is one of the non-psychoactive cannabinoids found inCannabis sativa and the second most abundant after cannabidiol. CBC has been shown to produce antinociception and anti-inflammatory effects in rodents; it is a major non-psychotropic phytocannabinoid that inhibits endocannabinoid inactivation and activates the transient receptor potential ankyrin-1 (TRPA1). The use of non-psychoactive cannabinoids as protective, ameliorative, and preventive agents in diverse disease pathophysiology is increasingly gaining global significance. Cannabidiol (CBD) has been extensively studied in this regard; howe...
Source: Comparative Clinical Pathology - June 22, 2023 Category: Pathology Source Type: research
The value of kidney biochemical parameters in diagnosis of acute tubular necrosis (ATN) in chickens
This study was designed to evaluate the effectiveness of biochemical factors such as blood urea nitrogen (BUN), creatinine, and serum uric acid in identifying and diagnosing acute tubular necrosis lesion in broilers. Kidney tissue and blood samples from 80 broiler chickens at the slaughter stage were collected. Tissue samples were collected in 10% formalin to detect pathological lesions. The components of BUN, creatinine, and serum uric acid were measured with commercially available kits. After preparing the histopathological sections, based on the percentage of necrosis of the renal tubules in each tissue sample, the path...
Source: Comparative Clinical Pathology - June 21, 2023 Category: Pathology Source Type: research
Neutrophil-to-Lymphocyte ratio and Monocyte-to-HDL ratio as a Biomarker of Urolithiasis
AbstractKidney stones are associated with increased chances of chronic kidney disorders, kidney failure, diabetes, hypertension, and cardiovascular disorders globally. This case –control study is designed to investigate the fluctuations in neutrophil-to-lymphocyte ratio (NLR) and monocyte-to-high-density lipid (HDL) ratio by comparing healthy subjects with cases suffering from kidney stones. Hematological and biochemical assays from 117 blood samples of cases and 117 cont rol, collected between October 2021 to June 2022, were inferred by applying statistical tests. Aftert-test, various hematological parameters of disease...
Source: Comparative Clinical Pathology - June 17, 2023 Category: Pathology Source Type: research
Melatonin mitigates oxidative stress in luniron-induced testicular injury in Wistar rats
This study aims to investigate the potential protective effects of melatonin (MLT) against linuron-induced testicular toxicity and spermatogenesis damage. Rats were divided into four groups: the control group (no treatment), the MLT group that received MLT (10 mg/kg b.w), the LIN group that received LIN (120 mg/kg b.w), and (LIN/MLT) group treated with LIN and MLT. The investigated substances MLT and linuron (LIN) were given orally to the animals for 30 days. The results showed that linuron treatment-induced testicular dysfunctions demonstrated significant inhibition of p ituitary–testicular axis hormone synthesis (di...
Source: Comparative Clinical Pathology - June 17, 2023 Category: Pathology Source Type: research
Inhalation of smoke from burning tire triggers oxidative stress and impairs liver and kidney functions in rats
This study investigated the toxicological effects of smoke from the burning tire in male Wistar rats. The experimental rats were assigned into five (5) groups of 5 rats in each group. Group 1 served as the normal control (not exposed to smokes from the burning tire), while groups 2 –5 were exposed to 10 mg/m3 PM10 smoke from burning tire once daily for 15, 30, 60, and 120 min, respectively, for 21 days. The rats were sacrificed, and the toxicity profile was measured by evaluating the hematological and biochemical parameters along with the liver, heart, kidney, and lung histology. Our results showed that smokes from t...
Source: Comparative Clinical Pathology - June 16, 2023 Category: Pathology Source Type: research
Taurine mitigates sodium arsenite –induced cardiorenal dysfunction in cockerel chicks: from toxicological, biochemical, and immunohistochemical stand-points
AbstractThe Heart and kidney are adversely affected by arsenic toxicity, a highly toxic environmental pollutant.The aim of this study was to evaluate cardio-renal protective effects of taurine against sodium arsenite –induced toxicity. Taurine (2-amino ethane sulphonic acid; TAU) is a major free intracellular amino acid in many mammalian tissues including the liver. Taurine supplementation has been documented to mitigate steatosis and hepatotoxicity in several animal models.Sixty cockerel chicks (1-day old) of uniform weight were randomly assigned into six groups of ten cockerel chicks: Group A, Control; Group B, Sodium ...
Source: Comparative Clinical Pathology - June 14, 2023 Category: Pathology Source Type: research
Protection of probiotic Bacillus coagulans and Lactobacillus plantarum against cadmium toxicity in rats
AbstractCadmium (Cd) is an environmental pollutant that can cause harmful effects on human and animal health due to its transfer to the food chain. Our previous work showed that probiotic candidates ofBacillus coagulans andLactobacillus plantarum can relieve mercury oral harmful effects in rats. Hence, this follow-up study investigated the effect of these two bacteria on chronic cadmium toxicity. Forty-eight Wistar rats have been studied for 48 days, so that cadmium receiving groups received 100 μg/mL of cadmium chloride per day through gavage. Daily administration of 1 × 109 CFU/spore/mL ofB. coagulans andL. pla...
Source: Comparative Clinical Pathology - June 14, 2023 Category: Pathology Source Type: research
Donepezil-based combination therapy for Alzheimer ’s disease and related neuropathies
AbstractCombination therapy has birthed improved treatment possibilities for a number of complex and degenerative diseases in the world, which include cancer, diabetes and cardiovascular disease, and has been encouraged for use in the treatment of Alzheimer ’s disease (AD), as a result of its complex pathological pathways. The combination of memantine and donepezil was approved for use to treat moderate-to-severe AD cases in 2014. Studies have however continued to investigate many other possible combination therapies for the treatment of AD. This art icle highlights some of the recent combination therapies involving the ...
Source: Comparative Clinical Pathology - June 9, 2023 Category: Pathology Source Type: research
Riboflavin potentially attenuates arsenic hepatotoxicity: a focus on oxidative changes, apoptosis, and PINK1 pathway
This study investigates riboflavin's hepatoprotection (a flavoproteins component) on toxicity induced by As2O3. Twenty-four male Wistar rats were randomly assigned into four equal groups, and the experimental group orally received As2O3 (3 mg/L/day) alone or in combination with riboflavin (40 mg/L/day) for 30 consecutive days. To detect liver abnormalities the major oxidative stress indices (reactive oxygen species and total antioxidative status), lipid peroxidation (malondialdehyde (MDA)), inflammatory markers (interleukin 6 and C -reactive protein), liver enzymes, and mRNA expression levels of apoptosis and PINK1 path...
Source: Comparative Clinical Pathology - June 6, 2023 Category: Pathology Source Type: research
Rare case of Thelazia infection in a Jersey cow —a report from Andhra Pradesh, India
AbstractThelazia spp. parasites are nematodes which are transmitted by arthropods causing ocular diseases in various domestic animals including humans. Adult worms are responsible for the development of clinical signs in mammals. A 6-year-old Jersey cross-bred cattle was presented to the Veterinary Hospital with bilateral lacrimation, and frequent rubbing of eyes on hard surfaces. Clinical examination revealed hyperemia of the conjunctiva and blepharospasm. The right eye showed the motile parasites at the inner canthus and was collected into normal saline. Confirmation of theThelazia was done by the morphology of the worm ...
Source: Comparative Clinical Pathology - June 5, 2023 Category: Pathology Source Type: research
Antidiabetic properties of Dioscoreophyllum cumminsii (Stapf) Diels stem bark on streptozotocin-induced diabetic rats
AbstractTraditionally,Dioscoreophyllum cumminsii has been reported for its ability to treat diabetes; however, the stem bark of the plant has not been scientifically exploited for its antidiabetic activity. The study aims to investigate the effect of the antidiabetic properties ofDioscoreophyllum cumminsii stem extract on streptozotocin-induced diabetic rats. Thirty rats were made diabetic by a single intraperitoneal administration of streptozocin (STZ), at a dose of 45 mg/kg bw. After confirming they are diabetic, 21 days of administration was conducted on the rats which are divided into 6 groups; Control (not diabetic...
Source: Comparative Clinical Pathology - June 1, 2023 Category: Pathology Source Type: research
Citrullus lanatus methanol seed extract exhibits antioxidant and anti-inflammatory potential against ethanol-induced kidney damage in Wistar rats
AbstractEthanol consumption can generate free radicals and cause oxidative stress, tissue inflammation, and kidney impairment. It has long been known that saponins from natural sources have numerous therapeutic benefits in African herbal and traditional medicine. However, the study aimed to the study aimed to evaluate the ameliorative effect of saponin-rich extract ofCitrullus lanatus seed against ethanol-induced kidney injury. Rats were divided into six groups: a normal control, an ethanol control (10 ml/kg of 50% ethanol), a saponin control (100 mg/kg/day), an ethanol plus low and high doses of saponin (ET + 50 and 100 m...
Source: Comparative Clinical Pathology - May 29, 2023 Category: Pathology Source Type: research
Echocardiographic and histopathological study on vegetative growth in the left ventricle of a buffalo
AbstractEndocarditis is inflammation of the endocardium of the heart, and it might be of infectious origin or vegetative origin. In buffaloes, most of the time, it will be a vegetative form mixed with bacteria, and its diagnosis in live animals is a challenge to the physician. An adult buffalo was presented to the clinic with a history of chronic loss of body weight, intermittent fever, dullness, lameness and loss of milk yield which was not responsive even after medications. Upon examination, it had fever, tachycardia, tachypnea, painful stance and a loud pounding heartbeat with cardiac murmurs. Blood analysis displayed a...
Source: Comparative Clinical Pathology - May 29, 2023 Category: Pathology Source Type: research
Profiles of plasma interleukin-6 and interleukin-10 in relation to some haematologic alterations following colo-peritoneo-cystoplasty in dogs
AbstractSystemic inflammatory response to surgical interventions has been characterized by an orchestrated interplay of endogenous mediatory cytokines and haematologic alterations. The study was carried out to determine the changes in plasma levels of pro-inflammatory interleukin-6 (IL-6) and anti-inflammatory interleukin-10 (IL-10) in relation to haematological alterations in response to colo-peritoneo-cystoplasty in dogs. Haematological and plasma concentrations of IL-6 and IL-10 were profiled in four apparently healthy dogs, following the colo-peritoneo-cystoplastic procedure. Mean packed cell volume (PCV) significantly...
Source: Comparative Clinical Pathology - May 19, 2023 Category: Pathology Source Type: research
Impact of Red Bull consumption on gastric acid and mucus secretion in Albino rats: Study of the biochemical and histopathological effects
AbstractNowadays energy drinks (EDs) are widely used among teenagers. One of the most popular EDs in Egypt is Red BullR (RB). The aim of this study was to evaluate the chronic effects of RB on gastric acidity, oxidative stress, and inflammation in pylorus-ligated rats. Thirty-two adult male albino rats were divided into 4 groups; Control animals were given distilled water instead of a beverage. RB was given in doses of 5, 7.5, and 10 ml/kg, p.o., twice daily for 28 days in pylorus-ligated rats. The effects of different doses of RB were investigated on body weight, gastric acidity, and volume and pH of gastric secretions...
Source: Comparative Clinical Pathology - May 16, 2023 Category: Pathology Source Type: research
