The effect of melatonin on expression of p53 and ovarian preantral follicle development isolated from vitrified ovary
AbstractOvarian vitrification is a strategy for conservation of fertility of young female patients, suffering from infertility, due to iatrogenic loss of ovarian function, resulting from chemotherapy and/or radiation therapy. The purpose of this study was to determine the influence of melatonin on expression of p53 and the developmental rate of preantral follicles isolated from vitrified and non-vitrified ovaries. This experimental study was carried out on 40, 14-day-old female mice (NMRI). One ovary from each mouse was used randomly for the vitrification procedure. Preantral follicles with a diameter of 120 –140&nbs...
Source: Comparative Clinical Pathology - October 2, 2017 Category: Pathology Source Type: research

Retrospective study on the etiology and clinical signs of canine otitis
AbstractThe survey comprised 5870 dogs with surgical diagnoses for the period 1 January 2010 to 31 December 2014, out of which 269 (4.58%) were diagnosed with otitis. The total number of isolates was 53: 17 coagulase-positive staphylococci, 2 beta-hemolytic staphylococci, 16Pseudomonas aeruginosa, 7Proteus mirabilis, 9Malassezia pachydermatis, and 2Candida spp. Bacterial and fungal infections were most frequently encountered in the summer (26; 49.06%) then in the autumn (13 isolates, 24.54%), and winter and spring (7 isolates, 13.21%). The highest number of isolates (21.4%) was resistant to chloramphenicol, followed by tho...
Source: Comparative Clinical Pathology - October 2, 2017 Category: Pathology Source Type: research

Preliminary use of Polygonum minus Linn. leaf extract on growth performance, feed utilization, and some hematological indices of Anabas testudineus (Bloch, 1792)
AbstractThe main objectives of the present study were to improve the growth performance, survival rate, and hematological indices of fingerlingAnabas testudineus, by usingPolygonum minus plant leaf extract, sprayed at four different levels (0 as the control group, 50, 70, and 100  ml/kg fish feed), for 60 days. The growth indices and survival rate were investigated at 15-day intervals, while hematological indices were evaluated at the end of experiment (60 days). The results showed that the weight gain (WG), average daily growth rate (ADG), specific growth rate (SGR), fee d efficiency (FE), and protein effic...
Source: Comparative Clinical Pathology - September 30, 2017 Category: Pathology Source Type: research

Comparison of SFRP2 promoter methylation in stool sample and cfDNA regarding patients with colorectal cancer
AbstractColorectal cancer (CRC) is one of the main causes of death in the world. Diagnosis in precancerous lesions stage is an important factor in reducing the mortality rate in CRC. Our study aimed at determining methylation status of secreted frizzled-related protein2 (SFRP2) gene in stool and serum samples of patients compared to healthy individuals. We collected serum and stool samples from 50 individuals including 25 patients with colorectal cancer and 25 healthy individuals. DNA was isolated from the serum and stool and treated by sodium bisulfite. It was then analyzed by methylation-specific polymerase chain reactio...
Source: Comparative Clinical Pathology - September 30, 2017 Category: Pathology Source Type: research

Clinical biochemistry profile of biventricular heart failure in a mixed-breed bitch
AbstractA six-year old mixed-breed bitch was presented for chronic ascites. A diagnostic work-up was crafted to ascertain the cause of fluid accumulation in the peritoneal cavity. Clinical biochemistry tests for status of the liver and kidneys were performed. Elevations in absolute concentrations of the liver enzymes aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were useful in evaluation of hepatocellular damage associated with right-sided heart failure. Normal levels of serum albumin and bilirubin, however, underscored the preserved functional capacity of the liver in spite of hepatocellular damage. Elev...
Source: Comparative Clinical Pathology - September 30, 2017 Category: Pathology Source Type: research

Atrial fibrillation associated with dexamethasone in dairy cattle
AbstractCorticosteroids are commonly administered to dairy cows with ketosis to enhance gluconeogenesis, but their use has also been associated with development of bovine hypokalemia syndrome (BHKS). Four-year-old Holstein dairy cattle presented with somnolence, depression, anorexia, weakness, sunken eye, pallor mucus membrane, and cardiac arrhythmia. Owner described that she suffered from dystocia and metritis that received broad spectrum antibiotic and ketofen for 4  days. Treatment continued with dexamethasone, vitamin B complex, and antibiotic for five consecutive days to resolve the problem but lethargy and disco...
Source: Comparative Clinical Pathology - September 30, 2017 Category: Pathology Source Type: research

The evaluation of the serum level of IL-10 in OLP patients
In this study, 44 serum samples from patients with lichen planus and 44 serum samples from healthy participants were collected during 2015 –2016. The patients with clinical or histopathological confirmation for oral lichen planus (OLP) were included in the case group, and the healthy patients were recruited in the control group. The serum level of IL-10 was measured by ELISA kits. In this cross-sectional study between 2015 and 2016, the serum level of IL-10 was measured in 53 females (60.22%) and 35 males (39.77%). In the lichen planus group, 44 subjects and in the control group, 44 healthy participants were enrolled...
Source: Comparative Clinical Pathology - September 30, 2017 Category: Pathology Source Type: research

Histopathologic study on mice infected with Plasmodium berghei after treatment with sulfadoxine-pyrimethamine, pyrimethamine, and hydroxychloroquine sulfate
AbstractMalaria, a disease caused by a parasitic protozoan belonging to the genusPlasmodium spp., is one of the common diseases in the tropical regions. Our aim in this study is to examine the effects of sulfadoxine-pyrimethamine, pyrimethamine, and hydroxychloroquine sulfate on the liver, spleen, and kidneys of treated mice withPlasmodium berghei. Mice were divided into five groups, of which three were infected with the parasite and treated with the aforementioned drugs. The other two groups were considered as negative (non-infected) and positive (infected) controls. The histopathological changes in the spleen among the d...
Source: Comparative Clinical Pathology - September 26, 2017 Category: Pathology Source Type: research

Effect of sodium levothyroxine on histomorphometry, histopathology, histochemistry, and immunohistochemistry of articular cartilage in female mice
AbstractSodium levothyroxine is one of the common medicines used for treatment of hypothyroidism. The aim of the present study was to assess the effect of sodium levothyroxine on the middle part of tibia plateau in knee joint and related hormones in individuals affected by hypothyroidism. Fifty female mice were randomized into five groups. The first group was considered as control; the second and third groups were received different doses of levothyroxine; the forth group was undergone hypothyroidism; and the fifth group, the animals with hypothyroidism, was received levothyroxine orally. After 8  weeks, serum samples...
Source: Comparative Clinical Pathology - September 21, 2017 Category: Pathology Source Type: research

Trypanosoma evansi infection in a cat —a rare case
AbstractA seventeen-month-old tom cat was presented to the hospital with the history of pyrexia, unable to stand with forelimbs and abnormal mentation.Trypanosoma evansi organisms were detected in the stained peripheral blood smears collected from the cat. The cat had anemia, hypoglycemia and hypoalbuminemia. The cat was successfully treated with injection diminazene aceturate along with other supportive medication and uneventfully recovery was noticed by the sixth day in his activities. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 18, 2017 Category: Pathology Source Type: research

Comparative evaluation of the effects of ezetimibe and atorvastatin on serum lipid profile changes in experimental hypercholesterolemia in cat
In conclusion, ezetimibe was more effective than atorvastatin in reducing cholesterol. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 17, 2017 Category: Pathology Source Type: research

Pulmonary tuberculosis: could tumor markers CA 15-3 and CA 125 be useful for diagnosis and evaluation of response to therapy?
AbstractDiagnosis and treatment evaluation of pulmonary tuberculosis (PTB) are time-consuming, so a non-conventional approach should be considered. Our study investigates the utility of CA125 and CA15-3 in differentiation of PTB from other pulmonary infections and prediction of response to antituberculous drugs. A prospective case control study was carried out on 69 patients: group A: 42 PTB and group B (control): 27 non-PTB patients. All patients were subjected to clinical, radiological, microbiological, and quantitative measurement of serum levels of CA125 and CA15-3 by ELIZA at time of diagnosis. Gp-A patients were ZN a...
Source: Comparative Clinical Pathology - September 15, 2017 Category: Pathology Source Type: research

The genetic association of cytokine genes, single nucleotide polymorphisms, and the incidence of liver cirrhosis in chronic hepatitis C Egyptian patients
The objective of this study were to evaluate the effect of proinflammatory cytokines (IL-18 − 607 C/A, rs 1946518; IL-18 − 137 G/C, rs 187283; INF-γ + 874 T/A, rs 240561), SNPs, and the anti-inflammatory cytokine (IL-10 + 1082 G/A, rs 1800896) SNPs on the risk of development of hepatic cirrhosis in Egyptian patients and to evaluate the combined effect of the polymorphic variants t hat show an interrelation with the risk of hepatic cirrhosis. The study included 63 chronic HCV patients. The patients were stratified into two groups. Group 1 consisted of 33 cirrhotic chronic hepatitis C pa...
Source: Comparative Clinical Pathology - September 15, 2017 Category: Pathology Source Type: research

Comparison of anthropometric measures in people with and without short- and long-term complications after laparoscopic sleeve gastrectomy
AbstractLaparoscopic sleeve gastrectomy (LSG) is a popular surgical procedure for obesity treatment. Prediction of post-operative complication occurrence can result in better outcomes. It is reported that anthropometric measures such as waist circumference (WC), hip circumference (HC), arm circumference (AC), waist-to-height ratio (WHtR), waist-to-hip ratio (WHR), and conicity index are more sensitive to predict the risk of obesity-related diseases than body mass index (BMI) and body weight (BW) are. No study has investigated the relationship between these anthropometric measures and complications after LSG in people with ...
Source: Comparative Clinical Pathology - September 14, 2017 Category: Pathology Source Type: research

Phenotypic variation of thymic epithelial cells and partial spontaneous regression in thymoma of a cow
AbstractThymoma was recognized in a 12-year-old Holstein dairy cow presenting with no significant clinical signs. The animal was conventionally slaughtered at an abattoir and, on postmortem examination, a spherical tumor mass was found in the anterior region of the mediastinum. Histologically, the tumor was fibrously encapsulated and exhibiting varying-sized cystoid cavities containing serous fluid and necrotic and hemorrhagic foci. No architectural features showing distinct characteristics to a sufficient degree to allow identification of the thymus or other organs could be seen. The tumor showed diffuse solid sheet-like ...
Source: Comparative Clinical Pathology - September 14, 2017 Category: Pathology Source Type: research

Serum 8-hydroxydeoxyguanosine and aldose reductase C-106T polymorphism in type 2 diabetes mellitus and its relation to complications in Egyptian patients
AbstractDiabetes mellitus (DM) is an inherited disease, which can affect every organ in the body. 8-hydroxydeoxyguanosine (8-OHdG) is a biomarker for oxidative DNA damage. Occurrence of C-106T polymorphism of the aldose reductase (ALR) gene in type II diabetic patients suffering from diabetic microvascular complications has been reported. The aim of the present work was to assess the association between oxidative stress and C(-106)T ALR gene polymorphism and the incident of type 2 diabetes (T2D) and its complications. The current study was conducted on 200 subjects classified into 150 T2D patients and 50 healthy control su...
Source: Comparative Clinical Pathology - September 14, 2017 Category: Pathology Source Type: research

Betulinic acid, natural pentacyclic triterpenoid prevents arsenic-induced nephrotoxicity in male Wistar rats
AbstractThe aim of this study was to investigate the possible renoprotective role of betulinic acid, a natural pentacyclic triterpenoid on arsenic-induced nephrotoxicity. Male Wistar rats were divided into four groups and experiment was done as follows: normal control group, arsenic trioxide (4  mg/kg b.wt) group, betulinic acid control (100 mg/kg b.wt) group, and a combination therapy group with both arsenic trioxide (4 mg/kg b.wt) and betulinic acid (100 mg/kg b.wt).The mode of administration of drugs was oral, and duration of study was 30 days. The results showed that the arsenic t reatment infl...
Source: Comparative Clinical Pathology - September 13, 2017 Category: Pathology Source Type: research

Ulcerative colitis: a phytomedical technical note
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 11, 2017 Category: Pathology Source Type: research

Comparative gene set enrichment analysis (GSEA) of the embryonic stem cell (ES) gene signatures in canine and human osteosarcoma
In this study, we sought to compare the expression profiles of embryonic stem cell (ES) gene signatures in canine and human OSA tissues and cell lines. The expression patterns of three major ES gene signatures (modules or gene sets)—namely Myc, Core, and PRC modules—were primarily analyzed through the gene set enrichment analysis method in three gene expression datasets. For verification of the primary results, an additional 13 ES gene sets which were categorized into four groups—nam ely NOS targets, ES expressed, Myc targets, and Polycomb targets—were evaluated in expression datasets. Additionally,...
Source: Comparative Clinical Pathology - September 10, 2017 Category: Pathology Source Type: research

Short- and long-term exposure to imidacloprid disturbs the elemental composition and free amino acid profile in muscles of Labeo rohita
AbstractImidacloprid [1-(6-chloro-3-pyridylmethyl)-N-nitro-imidazolidin-2-ylideneamine] is among the most extensively used pesticide in Pakistan. The aim of this investigation was to report the changes in concentration of different elements and amino acid in the muscle ofLabeo rohita upon imidacloprid exposure. JuvenileLabeo rohita (N = 480) were treated with 120mgL−1 imidacloprid under short and long-term experimental conditions. At the end of each experiment, fish muscle was used for elemental concentration (determination by an atomic absorption spectrophotometer) and amino acid estimation (by high-perfor...
Source: Comparative Clinical Pathology - September 9, 2017 Category: Pathology Source Type: research

Endobronchial lipoma, the initial evaluation and appropriate management
We present a 42-year-old woman with endobronchial lipoma and the initial evaluation and appropriate management. A 42-year-old woman presented with history of dry cough and wheeze for eight months was candidate for bronchoscopy study. Histopathology examination of exc ised lesion confirmed endobronchial lipoma of the left lower lobe bronchus. The post-bronchoscopy period was uneventful. The patient was disease free in the following three years. The physicians, especially at major referral lung centers, are much more likely to be familiar with endobronchial lipoma and its clinical awareness, because misdiagnosis can delay th...
Source: Comparative Clinical Pathology - September 9, 2017 Category: Pathology Source Type: research

Hemato-immunological and plasma biochemical responses of silvery-black porgy ( Sparidentex hasta ) fed protein and essential amino acid deficient diets
AbstractA 6-week feeding trial was conducted to evaluate the effects of protein free (PF) and essential amino acid deficient (EAAD) diets on the physiological responses of silvery-black porgy (Sparidentex hasta) juveniles. Three experimental diets were formulated: a control diet in which 60% of dietary nitrogen was provided by intact protein (fish meal) and 40% by crystalline AA [(blends of essential amino acids (EAAs) and non-essential amino acids (NEAAs)]; an essential amino acid deficient diet in which 60% of dietary N was provided by intact protein, whereas the rest was provided by NEAAs; and a protein free (PF) diet, ...
Source: Comparative Clinical Pathology - September 9, 2017 Category: Pathology Source Type: research

Influence of a device intravaginal to synchronization/induction of estrus and its reuse in sheep vaginal flora
The objective of this study was to characterize the vaginal bacterial flora and their antibiotic susceptibility in vitro after, application, and the reuse of an intravaginal progesterone device (CIDR) in controlling the estrous cycle in sheep. Thirty Texel ewe lambs were used, after carefully assessed on their health and body condition. Experimental groups were formed by new CIDR (CIDRn,n = 15) and CIDR reused (CIDRu,n = 15). Treatment with progesterone lasted 6 days. Sterile swabs were used for vaginal sample collection, a procedure performed previously of CIDR insertion and on the moment of CIDR ...
Source: Comparative Clinical Pathology - September 4, 2017 Category: Pathology Source Type: research

Effects of chronic exposure of X-ray on hematological parameters in human blood
AbstractThe aim of the study was to show how many X-ray technicians who work in radiology department at public-sector hospitals in Sulaimani city are affected by doses of X-ray from X-ray machines, fluoroscopy, and CT scan through examining 12 hematological parameters. The study group included 24 seemingly healthy female and male X-ray technicians. The group was subdivided into 12 female X-ray technicians having average ages of 39 and 14  years of 4 hourly per day of services. As well as, 12 male X-ray technicians having average ages of 41 and 15 years of 5 hourly per day of services. A group of 24 healthy contro...
Source: Comparative Clinical Pathology - September 2, 2017 Category: Pathology Source Type: research

Apoptosis induced by Moringa oleifera Lam. pod in mouse colon carcinoma model
AbstractThe aim of this study was to investigate the effect of boiledMoringa oleifera Lam. pod (bMO) in colon carcinogenesis via apoptotic pathway. Four groups of mice (eight mice/group) were induced by azoxymethane (AOM, 10  mg/kg BW i.p. at the third week) followed by dextran sodium sulfate (DSS) for 7 days (2% in drinking water at the fourth week), while one negative group (n = 8) received AIN-76A basal diet without AOM/DSS induction. Positive control group received basal diet with AOM/DSS induction, whereas treatment groups received basal diet containing 1.5%, 3.0%, and 6.0% of bMO at the 5th week t...
Source: Comparative Clinical Pathology - September 1, 2017 Category: Pathology Source Type: research

Application of bovine bone versus bovine DBM graft on bone healing of radial defect in rat
AbstractReconstruction of the fractured bone and bone healing biology is compound processes, and the effectiveness of osteosynthesis is determined by their success. Currently, autografts or allografts and mineral bone substitutes are used commonly for skeletal restoration. The aim of this study is to evaluate the effects of bovine demineralized bone matrix (DBM) and bovine bone (bovine xenografts) on the bone healing process in rat. Sixteen female Sprague-Dawley rats were used in separate four groups: autograft (positive control,n = 4), untreated defect (negative control,n = 4), bovine DBM (n =&nbs...
Source: Comparative Clinical Pathology - August 8, 2017 Category: Pathology Source Type: research

Antiechinococcal assessment of atovaquone —an in silico and in vitro analysis
AbstractCystic echinococcosis (CE), a hydatid disease, caused by larval form of dog tapewormEchiconoccus granulosus, is responsible for considerable human morbidity and mortality. The currently available drugs have to be applied in high doses for prolonged periods for treatment of CE, and have several side effects. Atovaquone, a naphthoquinone, has previously been shown to possess a very promising antiparasitic potential against malaria, toxoplasmosis, babesiasis, etc. by inhibiting parasite ’s electron transport chain, cytochrome bc1 complex. Thus, the present study was aimed to investigate the antiechinococcal pote...
Source: Comparative Clinical Pathology - August 7, 2017 Category: Pathology Source Type: research

Modulation of cholinergic, monoaminergic, and purinergic enzymes of the brain functions by bitter ( Vernonia amygdalina ) and water bitter ( Struchium sparganophora ) leaves extracts: comparison of phenolic constituents versus nootropic potentials
This study investigated the possible neuroprotective properties of two commonly consumed tropical vegetables. The modulatory effects ofVernonia amygdalina (VA) andStruchium sparganophora (SS) on the activities of Na+/K+ ATPase, ecto-5 ′-nucleotidase, monoamine oxidase (MAO), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and FeSO4-induced oxidative stress in rat brain homogenate were assessed. The result revealed that both vegetables inhibited AChE, BChE, MAO, and ecto-5 ’nucleotidase activities, but stimulated Na+/K+ ATPase activity in a concentration-dependent manner. The SS had a significantly hi...
Source: Comparative Clinical Pathology - August 5, 2017 Category: Pathology Source Type: research

Blood chemistry, haematology and ileal bacterial counts of broilers fed fermented mixture of cassava root meal and palm kernel cake
AbstractA study on blood chemistry, haematology and ileal bacterial count of broilers fed fermented mixture of grated cassava tubers and palm kernel cake (FCP-mix) as a replacement for maize at 0, 25, 50, 75 and 100% levels of inclusion was undertaken. One hundred and eighty (180) 7-day-old broiler chickens were divided into six groups of 30 birds, and each group replicated thrice. Six experimental diets were formulated with diet 1 containing maize as the major energy source, while diet 6 containing fermented cassava root meal (CRM) and palm kernel cake (PKC) at the ratio of 1:1 as the main energy source. Diets 2, 3, 4 and...
Source: Comparative Clinical Pathology - July 31, 2017 Category: Pathology Source Type: research

Simultaneous occurrence of fibroblastic osteosarcoma and hypertrophic osteopathy (Marie ’s disease) in a German shepherd dog
AbstractOsteosarcoma, as a progressive tumor, accounts for approximately 80% of malignant bone tumors in dogs. Since it is a highly metastatic tumor, it develops pulmonary metastasis in the majority of cases. The considerable site preference of the tumor in dogs and the possibility of hypertrophic osteopathy in a number of cases must be considered. Clinical examination of a 12-year female German shepherd dog with lameness, weakness, and cachexia revealed an abnormal thickening in the left forelimb and a severe swelling in the left proximal humerus. Radiographic examinations showed severe swelling in the arm ’s soft t...
Source: Comparative Clinical Pathology - July 27, 2017 Category: Pathology Source Type: research

Prevalence and histopathological characteristics of Linguatula serrata infection among slaughtered ruminants in Mazandaran Province, northern Iran
AbstractThere is no data concerning the status ofLinguatula serrata (L. serrata) infection in ruminants in Mazandaran Province, northern Iran. Therefore, the present study was aimed to investigate the prevalence and pathologic features of linguatulosis, among slaughtered ruminants in several abattoirs in Mazandaran Province. The infection rate ofL. serrata in mesenteric lymph nodes of sheep, goats, and cattle were grossly and microscopically examined. Overall, of 6249 goats, sheep, and cattle examined, 16.8% of goats, 6.8% of sheep, and 6.9% of cattle were found to be infected withL. serrata nymphs. In general, the prevale...
Source: Comparative Clinical Pathology - July 24, 2017 Category: Pathology Source Type: research

Histomorphological and stereological study on the adrenal glands of adult female guinea fowl ( Numida meleagris )
AbstractAdrenal gland is one of the most important glands of the endocrine system, located in the anterior pole of kidney and secretes important hormones that play roles in carbohydrate, water and electrolyte metabolism and maintain the blood pressure in birds. Since the available information regarding the histology and stereology of adrenal gland are limited, this study was conducted to determine the histomorphology and stereology of this gland. For this purpose, 8 adult helmeted guinea fowls were killed humanely and used. Their adrenals were removed and immersed in buffer formalin 10%. Then, tissue sections were prepared...
Source: Comparative Clinical Pathology - July 24, 2017 Category: Pathology Source Type: research

Biomaterials, substitutes, and tissue engineering in bone repair: current and future concepts
AbstractBone is a complex, constantly changing organ comprised of mineralized hard tissue. This important structural component of vertebrate ’s body serves a variety of functions. Healthy bone system is essential for lifelong execution of these functions. Millions of people worldwide suffer from bone defects due to various reasons, including trauma, tumor, bone diseases, congenital defects, and aging. These defects are increasingly bec oming the majority of the clinical cases in orthopedics. For all the aforementioned cases in which the normal process of bone regeneration is either impaired or simply insufficient, th...
Source: Comparative Clinical Pathology - July 24, 2017 Category: Pathology Source Type: research

The effect of tocilizumab on clinical signs and laboratory findings in rheumatoid arthritis patients resistant to methotrexate
AbstractRheumatoid arthritis (RA) is a chronic systemic autoimmune inflammatory disease that mainly affects joint damage followed by increased morbidity and mortality rates. The progressive increase of inflammation factors, especially IL-6, is seen during the course of this condition. The aim of this study was to evaluate the effect of tocilizumab (TCZ) in patients with severe to moderate resistant RA with an inadequate response to treatment with disease-modifying antirheumatic drugs (DMARDs). We also reviewed the safety of the drug and related complications. Twelve selected RA patients received 8  mg/kg of TCZ every ...
Source: Comparative Clinical Pathology - July 19, 2017 Category: Pathology Source Type: research

Histologically proved cytomegalovirus as a terrible and neglect disease: a 13-year report of gastrointestinal and hepatobiliary manifestations from single referral center
AbstractThe aim of the present study was to evaluate the gastrointestinal and hepatobiliary manifestation of cytomegalovirus. In a retrospective study, demographic and clinical information of patients with gastrointestinal or hepatobiliary manifestations and histologically confirmed cytomegalovirus infection was investigated among pathological records. The association between serum cytomegalovirus pp65 and blood positivity of cytomegalovirus polymerase chain reaction with histological findings was also evaluated. Of the 74 included patients, 27 cases (36.5%) were female. The most common symptoms were diarrhea (24.3%). Unus...
Source: Comparative Clinical Pathology - July 18, 2017 Category: Pathology Source Type: research

In vitro radical scavenging potential and toxicopathological implications of administration of aqueous leaf extract of Eucalyptus obliqua L.Her. (Myrtaceae) in Wistar rats
This study evaluated the in vitro antioxidant potential and toxicological effects of aqueous leaf extract ofEucalyptus obliqua via acute and 28-day once daily treatments in Wistar rats. In the acute toxicity test, rats were orally administered the extract up to 5000  mg/kg. The sub-chronic toxicity study was performed by once daily oral administration with the extract at 200, 400, 500, and 1000 mg/kg body weight for 28 days. The control rats received sterile distilled water. Biochemical and hematological indices were determined and a histopathological examin ation was carried out. The extract showed marked a...
Source: Comparative Clinical Pathology - July 17, 2017 Category: Pathology Source Type: research

FGFR3, a marker suggestive of favorable prognosis in urothelial carcinoma
In conclusion, high expression of FGFR3 in urothelial carcinomas with statistically significant relationships regarding its expression levels with parameters of favorable prognosis like low grade and pTa stage, presence of papillary architecture, and lower-stage urothelial carcinomas rai sed the probability that FGFR3 expression may have a role in early development of urothelial neoplasia. Further studies are required to investigate the applicability of FGFR3-targeted agents as an additional treatment regimen in patients with FGFR3 overexpression. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 9, 2017 Category: Pathology Source Type: research

Enterobius vermicularis infection among appendectomy specimens in Qom Province, Central Iran: a retrospective study
AbstractThe role of parasites in the pathogenesis of appendicitis has long been discussed.Enterobius vermicularis (E. vermicularis) is the most common parasitic worm found in the removed appendices. The main objective of this study was to determine the prevalence of parasitic infections among appendectomy specimens in Qom Province, Central Iran. This retrospective study was conducted at Nekoii Hospital of Qom City, from 21 March 2005 to 19 March 2016 (11-year period). The histopathology reports and recorded demographic data concerning 13,744 performed appendectomies were reviewed and then extracted using a structured templ...
Source: Comparative Clinical Pathology - July 9, 2017 Category: Pathology Source Type: research

Blue-black grassquit ( Volatina jacarina ) leucocyte and average weight reference values
AbstractThe bird speciesVolatina jacarina is popularly known as blue-black grassquit. They are widely distributed geographically and are not considered threatened for extinction. They are popularly domesticated because of their pleasant singing they can be found in many households. That means Veterinarians are sometimes confronted with those animals. This requires a certain knowledge about these birds, including in terms of reference values. Due to the lack of hematological data for this species, the present study aimed to calculate reference values for leukocytes and mean weight for this bird. To this end, 13 blue-black g...
Source: Comparative Clinical Pathology - July 9, 2017 Category: Pathology Source Type: research

Ultrastructure of bioscaffolds derived from bovine articular cartilage as an experimental model to support blastema cells
AbstractDecellularized cartilage extracellular matrix (ECM)-derived bioscaffolds have been designed as they provide a unique opportunity to carry out research on the mechanisms of chondrogenesis and its regulation. These scaffolds are used as a model in order to emulate different aspects relating to the formation, degeneration, and regeneration of the cartilage. This project has studied the interaction between the decellularized bovine articular cartilage and the blastema cells derived from the punched rabbit ’s ear. Articular cartilage was dissected in fragments with 10 mm length and 2 mm thickness. Then, ...
Source: Comparative Clinical Pathology - June 24, 2017 Category: Pathology Source Type: research

Report and Abstracts for 17 th Spring ACCP Conference with Training Workshops, and Technology Exhibits
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 20, 2017 Category: Pathology Source Type: research

Effects of age on the reproductive performance of different males and females in bighead carp Hypophthalmichthys nobilis (Richardson, 1845)
AbstractThe age of fish can affect reproductive performance in relation to the gamete quality and productivity which might be expected to be changed in older individuals. The consideration can be important in artificial propagation in relation to the selection of broodstock. We determined the age-related changes in male and female reproductive characteristics of the bighead carpHypophthalmichthys nobilis and then we examined the effects of age on the male and female reproductive fitness in terms of fertilization, hatching, and larvae survival rate. A total of eight 3 –4-year-old males and eight 4–5-year-old fem...
Source: Comparative Clinical Pathology - June 19, 2017 Category: Pathology Source Type: research

Vitrification of bovine ovarian tissue: effect of perforated antral follicles on the structural preservation of follicles
The objective was to evaluate whether follicular perforation could improve the survival of antral follicles after vitrification. The present study was conducted on 20 bovine ovaries collected at slaughterhouse and a total of 344 antral follicles (less than 4  mm in diameter). For each ovary, three slices (approximately 1 × 1 cm) were separated from the cortex of each ovary and randomly were assigned in three groups, control (without freezing, fixed in 10% buffered formalin), non­perforated vitrified and perforated vitrified (after exposure to equi libration solution, antral follicles were imme...
Source: Comparative Clinical Pathology - June 17, 2017 Category: Pathology Source Type: research

Comparative and analytical study on active toxoplasmosis to assess the IgG avidity in correlation to serological profile in a cohort of Egyptian patients
AbstractProper diagnosis of toxoplasmosis is very important for proper treatment in manifested cases and to prevent congenital toxoplasmosis in case of pregnancy. Screening of 180 pregnant females by automated immune assay forToxoplasma IgG and IgM Abs was done first then positive cases and some of negative cases were evaluated for theToxoplasma IgG avidity test versusToxoplasma IgM and IgA Abs using ELISA technique for the detection of active toxoplasmosis during gestation using IHA rising titer as reference test. Assessment ofToxoplasma IgM by ELISA compared with rising titer of IHA as a reference test revealed that the ...
Source: Comparative Clinical Pathology - June 17, 2017 Category: Pathology Source Type: research

Are surface markers of cancer stem cells reliable factors for cancer treatment follow-up?
AbstractRecent studies have explained that cancer stem cells (CSCs) can both instigate and maintain tumours. As a result, they are a good choice for targeted therapy in the treatment of aggressive cancers, and their long-term assessment is essential for the follow-up of therapeutic agent efficacy. However, several studies have revealed that their detection on the basis of surface markers is challenging. In this area, there are two main questions. First, why are surface markers of the CSCs so difficult to detect in in vitro studies? Second, are CSCs ’ surface markers reliable factors when assessing cancer treatment ef...
Source: Comparative Clinical Pathology - June 16, 2017 Category: Pathology Source Type: research

Level of cystatin C in functional thyroid disorders and its relation to GFR
AbstractThyroid hormones affect the development and physiology of the kidneys. Hypothyroidism and hyperthyroidism affect glomerular filtration rate (GFR), tubular function, renal blood flow, and structure of the kidney. Cystatin C is a non-glycosylated neuroendocrine protein. It is freely filtered at the glomerulus and completely reabsorbed and catabolized by cells of tubules. Cystatin C levels, affected by the thyroid state, is being increased in hyperthyroidism and decreased in hypothyroidism. The current study was conducted on 66 patients of thyroid disorder and 21 healthy control subjects. Total T3, total T4, and TSH w...
Source: Comparative Clinical Pathology - June 6, 2017 Category: Pathology Source Type: research

Evaluation of haematological and serum biochemical changes associated with constant rate infusion tramadol hydrochloride as an adjunct to ketoprofen in laparotomized and ovariohysterectomized dogs
This study evaluated changes in haematology and serum biochemistry associated with constant rate infusion (CRI) tramadol hydrochloride as an adjunct to ketoprofen for analgesia in adult female dogs following laparotomy (LP) and ovariohysterectomy (OVH). Thirty (30) adult female dogs randomly assigned into six groups of five dogs each were used in studies 1 and 2. In both studies, group 3 served as the control which received normal saline while groups 1 and 2 were given 1.0 and 2.0  mg/kg/h of tramadol Hcl, respectively. Dogs in study 1 were laparotomized while OVH was performed on dogs in study 2. Ketoprofen (4 m...
Source: Comparative Clinical Pathology - June 3, 2017 Category: Pathology Source Type: research

Association between beta globin haplotypes, HBBP1 and HMOX1 polymorphisms in relation to HbF among sickle cell anemia patients: a study in Southwest Iran
In this study, rs2071348 and rs2071746 polymorphisms in HBBP1 and heme oxygenase 1 (HMOX1) genes associated with different haplotypes and HbF levels in patients with sickle cell anemia were evaluated in comparison with healthy subjects. In this case-control study, 150 patients with sickle cell anemi a (90 homozygous and 60 sickle-cell trait) were evaluated and compared with 50 healthy individuals referred to Shafa tertiary hospital in Ahvaz city of Khuzestan Province, Iran. HbF level was measured by alkali and acid electrophoresis. PCR-RFLP and ARMS-PCR methods were used to determine β-globin gene haplotypes and HBBP1...
Source: Comparative Clinical Pathology - May 31, 2017 Category: Pathology Source Type: research

Effects of Nigerian red propolis in rats infected with Trypanosoma brucei brucei
AbstractPropolis has a long history of use in folk medicine and possesses various biological activities. Effects of the methanol extract of Nigerian red propolis (NRP) in trypanosome-infected rats were studied. Mature albino Wistar rats (30) were randomly grouped into six (A –F). Groups A–E were infected with 1.0 × 106Trypanosoma brucei brucei organisms intraperitoneally. Group F was uninfected. On establishment of infection, groups A –C were treated orally with NRP at 600, 400 and 200 mg/kg body weight for five consecutive days while group D received diminazene aceturate at 7 mg...
Source: Comparative Clinical Pathology - May 31, 2017 Category: Pathology Source Type: research

Modulation of immunity and inflammatory gene expression in buffalo ( Bubalus bubalis ) with some digestive disorders
AbstractSo far, there has been scarce information about the status of immunoglobulins (Ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. The purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. For this reason, 50 native breed water buffaloes were studied. Forty of these buffaloes showed various symptoms of digestive disorders and were allocated into 4 groups of equal sizes (group 1: uncategorized stomatitis; group 2: acute traumatic reticuloperitonitis [TRR]; gro...
Source: Comparative Clinical Pathology - May 31, 2017 Category: Pathology Source Type: research