Immunohistochemical and molecular detection of avian neoplastic disease viruses in layer chickens from poultry farms in Northwestern and Northcentral Nigeria
AbstractViral-induced neoplasms in poultry include Marek ’s disease (MD), avian leukosis (AL), and reticuloendotheliosis (RE). Many times, a presumptive diagnosis can be made based on clinical history and histopathology. However, cases of avian neoplasms due to mixed infection of the viruses are common occurrences in the field, which can only be diagnos ed using advanced diagnostic methods, hence the need for this study. A 7 and 10 formalin-fixed neoplastic livers from farms in Northwest (Kaduna) and Northcentral (Plateau) states of Nigeria were screened for the presence of Marek’s disease virus (MDV), avian leukosis v...
Source: Comparative Clinical Pathology - June 28, 2022 Category: Pathology Source Type: research
The effects of blood collection tube components on canine routine biochemistry and hormone assays
In conclusion, most analytes were stable in all blood collection tubes. The serum samples with or without gel and clot a ctivators had advantages over heparinized plasma for measurement of some analytes investigated in this study, with the exception of potassium. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 24, 2022 Category: Pathology Source Type: research
Retraction Note to: Determination of histopathological and biomedical parameters in protective effects of Petroselinum crispum on hepatotoxicity induced by dichlorvos in male Wistar rats
This article has been retracted. Please see the Retraction Notice for more detail:https://doi.org/10.1007/s00580-022-03370-0. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 23, 2022 Category: Pathology Source Type: research
Reproductive hormonal profile in Eastern Pacific green turtles captured in-water
This study determined the values of reproductive hormones and total thyroxine of 56 resident foraging green turtles captured between 2012 and 2014 at Golfo Dulce, Costa Rica. All of them underwent a physical examination and were deemed as healthy individuals. The progesterone values were 0.31 –2.723 ng/ml, estradiol 25.54–104.129 pg/ml, testosterone 1.94–228.97 ng/dl, and thyroxine 0.5–0.9 µg/dl. Since our population did not show sexual dimorphism, and the curved carapace length sizes did not match previous reports, turtles’ sex was determined based on high testosterone co ncentrations. Our results showed th...
Source: Comparative Clinical Pathology - June 17, 2022 Category: Pathology Source Type: research
Effects benefits and hazards of honey bee venom on wound healing and serum biochemical changes in alloxan-induced diabetic rats
This study investigated the therapeutic and side effects of systemic and local exposure to BV on cutaneous wound healing and serum biochemical changes in alloxan-induced diabetic rats. This experimental study was performed on 60 male Wistar rats randomly designated into three groups: control, diabetes (induced with alloxan), and diabetic treatment groups with subcutaneous and topically applied BV (0.5 mg/kg body weight [b.wt] and 0.25% concentration once a day for 21 days). The wound surface area was measured on days 4, 7, 14, and 21, and sampling was performed to evaluate wound healing prior to euthanization. At the en...
Source: Comparative Clinical Pathology - June 16, 2022 Category: Pathology Source Type: research
The modulatory effects of ketoconazole on cytokine changes induced by selective surgical stress in male rats
In this study, the levels of serum cytokines including IL-2, TNF-α, and IFN-γ were evaluated following the use of ketoconazole and glucocorticoids (derived from HPA axis) synthesis inhibition. After th e adaptation, thirty-six adult male rats were divided equally into three groups as follows: control, K15, and K30. These groups received normal saline, 15 mg/kg bw ketoconazole, and 30 mg/kg bw ketoconazole orally for 3 days every 24 h, respectively. The animals were anesthetized with propofol ( 100 mg/kg) and midazolam (3 mg/kg); then laparotomy, as a minor surgery, was performed. Four animals from each group were a...
Source: Comparative Clinical Pathology - June 11, 2022 Category: Pathology Source Type: research
Mechanisms underlying the hepatoprotective effect of silymarin on fluoxetine-induced liver injury in rats: the implication of peroxisome proliferator –activated receptor-gamma (PPAR-γ)
AbstractFluoxetine (FLX) is used widely as a first-line treatment in cases of depression and other neuropsychiatric disorders. Although its safety has been approved, the use of FLX was associated with liver injury and chronic liver disease. Silymarin (SIL) possesses antioxidant and anti-inflammatory effects. Hence, this study aimed to evaluate the protective effects of SIL on FLX-induced liver damage, implying the role of peroxisome proliferator-activated receptor-gamma (PPAR- γ). Rats were randomized to four groups: control group, SIL group (100 mg/kg/day; orally), FLX group (10 mg/kg/ day; orally), and SIL + FLX g...
Source: Comparative Clinical Pathology - June 9, 2022 Category: Pathology Source Type: research
Mucocutaneous Bowen ’s disease with a cutaneous horn overgrowth in a German shepherd dog
This report presents a case of canine mucocutaneous Bowen ’s disease developing underneath a cutaneous horn in the anal region. A 7-month-old female German shepherd was diagnosed with two fused cutaneous horns in the anal region. Macroscopically, two conical, exophytic horn-like tissues were seen, fused, and sharing an irregular basal soft tissue, posses sing parallel longitudinal grooves. Histopathologically, it was composed of a deep verrucous hyperkeratotic zone of squamous cell carcinoma in situ (Bowen’s disease) and an overlying cutaneous horn. Marked orthokeratosis, hypergranulosis, cellular basophilia and vacuol...
Source: Comparative Clinical Pathology - June 9, 2022 Category: Pathology Source Type: research
Bone healing differences in sexes under ibuprofen treatment
AbstractBone healing is comprised of a complex cascade of events to repair the fractured bone and gain functionality. This process is affected by numerous local and systemic factors such as the degree and extent of the injury, cardiovascular disorders, age, sex, chronic diseases, nutritional status, stimulants, and drugs used. The current study aimed to resolve the relationship of ibuprofen treatment and sex-specific bone healing in a rat model of the tibial fraction. The bone defect was inflicted by a tibial monocortical osteotomy with a 1-mm diameter. Male and female rats were injected with saline or ibuprofen (30 mg/k...
Source: Comparative Clinical Pathology - June 8, 2022 Category: Pathology Source Type: research
Stereological and functional study on ovarian follicles in surri mice following soybean consumption
The objective was to evaluate the morphometric characteristics of the ovaries followed by consumption of soybean through the precise and non-convergent technique of stereology, in immature animals. Thirty adult NMRI female mice at the age of 3 weeks were divided into three groups including the control, the experimental 1, and the experimental 2. The animals were fed a diet prepared by a nutritionist for 3 months. The control group received a soy-free diet and the experimental groups 1 and 2 received a diet that provided 15 and 35% of s oybean as the protein content, respectively. Serum levels of the hormones estrogen, p...
Source: Comparative Clinical Pathology - June 7, 2022 Category: Pathology Source Type: research
Ovarian follicular atresia and uterine toxicity after subchronic oral exposure of postpubertal rats to sodium arsenite
This study was designed to explore the extent of damages of sodium arsenite on female reproductive system of rats. Forty adult Wistar nulliparous rats having 3 months of age were grouped into four where the treated groups (Gr II, III, and IV) were gavaged with sodium arsenite (1, 3, and 5 mg/kg/day, respectively) for 30 consecutive days and a vehicle-fed group (Gr I) served as control. The treatment resulted in decrease of body weight (Gr III and IV) an d weight of ovary and uterus (Gr II, III, and IV). Marked decrease of the pre-antrum, antral, and Graafian follicle pools with concomitant enhancement of follicular atre...
Source: Comparative Clinical Pathology - June 3, 2022 Category: Pathology Source Type: research
Evaluating the hemato-biochemical indices in relation to the different etiologies of chronic diarrhea in dairy cattle and buffalo
AbstractVarious studies show that chronic diarrhea is a severe pathological condition that poses great challenge to veterinarians as well as cattle owners. Keeping in view the recent surge in the cases of chronic diarrhea due to multiple factors, a study was undertaken to understand the hemato-biochemical features vis- à-vis etiology of chronic diarrhea in dairy cattle and buffalo. The study was carried on adult dairy animals (n = 102) with the complaint of diarrhea from more than 1-month duration. The results reveal that 60% of dairy cattle and buffaloes had diarrhea from 1 to 3 months, 20% cases had diarrhea from 4...
Source: Comparative Clinical Pathology - June 3, 2022 Category: Pathology Source Type: research
The effect of high-intensity interval training and remote ischemic preconditioning on hematological parameters in middle-aged male Wistar rats
AbstractThe aim of this study was to examine the effects of high-intensity interval training and remote ischemic preconditioning on hematological blood markers in middle-aged male Wistar rats. Twenty-four male Wistar rats (12-month-old) were randomly assigned to four groups: high-intensity interval training (H), remote ischemic preconditioning + high-intensity interval training (RH), sham-high-intensity interval training (SH), and sham-remote ischemic preconditioning + high-intensity interval training (SRH). The rats in the experimental groups were administered the selected protocols 3 days a week for 21 days. T...
Source: Comparative Clinical Pathology - May 30, 2022 Category: Pathology Source Type: research
Comparative performance of CDC-modified SARS-CoV-2 real-time PCR assay with four different commercial assays: laboratory-based study
AbstractThe coronavirus infectious disease (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses. The pandemic has emerged as a global public health crisis, and the threat of fast-spreading of the latest variants of the coronavirus (such as omicron, delta) is rampant. Therefore, a fast and reliable diagnostic assay is needed to make the clinical decision for further treatment. The study aims to develop a Centers for Disease and Prevention (CDC) –modified qualitative real-time reverse transcriptase PCR (RT-qPCR) assay and parallel assessment of commercially available RT-qPCR assay (A...
Source: Comparative Clinical Pathology - May 26, 2022 Category: Pathology Source Type: research
Evaluation of the effect of short-chain organic acids and probiotics on production performance, egg white quality, and fecal microbiota of laying hens
This study investigated the effect of concomitant use of short-chain organic acids (acetic acid, butyric acid, and propionic acid) and probiotics mixture on the production performance, quality parameters of eggs produced, and fecal microbiota in laying hens. The project was carried out with 320 laying hens at 47 weeks. This experiment was a fully randomized 4 × 2 factorial experiment consisting of 4 levels of organic acid (zero, 0.1% propionic acid, 0.2% butyric acid, and 0.3% acetic acid) and 2 levels of probiotics mixture (zero and 0.01%) with 5 replications and 8 laying hens per replication. The r esults showed t...
Source: Comparative Clinical Pathology - May 25, 2022 Category: Pathology Source Type: research
