Comparative biochemical, parasitological, and histopathological studies on cystic echinococcosis in infected sheep
This study was done to investigate the parasitological studies and biochemical and histopathological changes of hydatid cyst infection in livers of sheep. Our study was carried out prospectively on sheep slaughtered in a southern slaughterhouse, Hail city, conducted from January 2015 to December 2015 to determine the prevalence, cyst viability, and biochemical analysis of fertile and infertile hydatid fluids. Out of 53,981 slaughtered sheep, 3811 were found infected with hydatid cyst; specifically, hydatid cyst was detected in the liver of sheep. The overall infection rate in slaughtered sheep in this period (from 1 Januar...
Source: Comparative Clinical Pathology - March 14, 2017 Category: Pathology Source Type: research

Erythrogram, leukogram, and acute phase protein reference intervals for healthy newborn Murrah buffalo calves ( Bubalus bubalis ) within the first month of life
In conclusion, hematologic and acute phase protein RI have been documented and can be used as a physiologic database to help the i nterpretation of laboratory results of newborn buffaloes during infection and inflammation conditions. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 11, 2017 Category: Pathology Source Type: research

Does ketamine-midazolam combination act as a routine and safe chemical restraint in cats? Clinical and hemato-biochemical evaluation
AbstractIntramuscular administration of ketamine-midazolam was used as a fast-acting chemical restraint, with convenient use of the drug in domestic cats. Furthermore, biochemical and hematological changes were evaluated following administration. A total of 20 clinically healthy cats were selected. During the physical examination, cephalic vein was prepared aseptically. Then, cats were restrained cautiously and intravenous catheter was placed in the cephalic vein and immediately heparinized. After covering and protecting the catheter, the animals were kept in separate cages for half an hour. Subsequently, the first blood s...
Source: Comparative Clinical Pathology - March 9, 2017 Category: Pathology Source Type: research

Expression of β-defensins in bovine oviduct and uterus during follicular and luteal phases
This study was conducted to examine the expression of β-defensins of cow’s reproductive tract and their relationship with estrus cycle using quantitative PCR and ELISA methods. Six kinds of β-defensins and the receptors of estrogen and progesterone were studied in cows’ reproductive tract during the follicular and luteal phases. The results show that besides LAP, TAP, BNBD4, and BNBD5, BNBD1 and BNBD10 were also found in cow oviduct, uterine horn, and uterine body. During the follicular phase, the uterine body had the highest expressions of BNBDI, TAP, LAP1, BNBD4, and BNBD5. However, the highest expr...
Source: Comparative Clinical Pathology - March 9, 2017 Category: Pathology Source Type: research

Ca 2+ sensitivity of skinned ventricular cardiac muscle and expression of cardiac myosin heavy chain isoforms in hibernating versus active frogs
This study was designed to find the changes in myosin heavy chain (MHC) isoforms and their relationship to calcium sensitivity in frog ventricular muscle during hibernation. MHC profile was determined by SDS-4% polyacrylamide gel electrophoresis in active and hibernating frogs for different intervals of time (10, 20, 30, and 60  days). Calcium sensitivity of Triton-skinned ventricular muscle fiber bundles was determined. Compared with active and hibernated frogs for 10 and 20 days, hibernated frogs for 30 and 60 days expressed significant amount (p 
Source: Comparative Clinical Pathology - March 9, 2017 Category: Pathology Source Type: research

Evaluation of immunochromatographic point-of-care testing in diagnosis of pulmonary tuberculosis
This study was conducted on 41 patients with pulmonary tuberculosis (PTB) and 35 healthy blood donors as control group. In addition to TST, sputum stained smear and TB culture, sera from patients and control groups were tested for reactivity against anti-38-16  kDa proteins with immunochromatographic point-of-care test. The number of culture proven, smear positive patients and TST reactors were 32 (78%), 25 (61%), and 32 (78%) respectively. Only 14 (34.1%) patients had positive results with anti-38-16 kDa antibodies detection test, while all control ser a were negative. The sensitivity, specificity, PPV, NPV, and...
Source: Comparative Clinical Pathology - March 7, 2017 Category: Pathology Source Type: research

Butyrylcholinesterase blood plasma concentrations of Common Swifts ( Apus apus )
AbstractButyrylcholinesterase (BChE), known as pseudocholinesterase or plasma cholinesterase, is a non-specific cholinesterase enzyme synthesised in the liver. The function of this enzyme is unknown in detail and is primarily discussed as a safeguarding system for Acetylcholinesterase (AChE). In a retrospective analysis of results of blood chemistry investigations of wild bird patients of the Clinic for Small Mammals, Reptiles and Birds, University of Veterinary Medicine Hannover, Foundation, Germany, BChE plasma concentrations of the Common Swift (Apus apus) and a possible influence of the body condition and age were dete...
Source: Comparative Clinical Pathology - March 2, 2017 Category: Pathology Source Type: research

The effect of chronic noise stress on serum levels of cortisol, gonadotropins, and sexual hormones at implantation time of mice
This study was designed to examine the effects of noise stress on serum levels of follicular stimulating hormone (FSH), luteinizing hormone (LH), corticosterone, estrogen, and progesterone of mature female NMARI mice at implantation time. In this experimental study, 30 mature female mice were divided into three equal groups. Pseudopregnancy was induced in all groups. The experimental groups 1 and 2 were exposed to noise. After 30  days that noise stress was induced, pseudopregnancy was performed. The experimental groups were exposed to noise stress for 4.5 days. At the end of the last exposure, the animals were a...
Source: Comparative Clinical Pathology - March 2, 2017 Category: Pathology Source Type: research

Diagnostic value of neutrophil-lymphocyte ratio versus C-reactive protein in discrimination between different pathogens causing community-acquired pneumonia
AbstractEarly discrimination of the causative organisms of community-acquired pneumonia (CAP) is important. The aim of the present study is to evaluate neutrophil –lymphocyte ratio (NLR) and derived NLR (dNLR) as adjunct tests to differentiate different types of CAP. NLR, dNLR, and C-reactive protein (CRP) were measured in 187 patients with CAP. Identification of causative organisms was done by direct smears, microbiological cultures, VITEK 2 compact system , and PCR of patient’s sputa together with detection of specific IgM antibodies againstChlamydia pneumoniae. There was a significant increase of NLR and CRP...
Source: Comparative Clinical Pathology - February 28, 2017 Category: Pathology Source Type: research

Clinical and paraclinical effects of transportation on miniature and domestic donkeys
AbstractTransportation of live animals is an inevitable husbandry practice and has been identified as one of the main causes of stress. Miniature donkeys, because of their rarity and entertainment value, occupy a unique place among all domesticated animals in some countries. We hypothesized that transportation stress would produce changes in clinical and biochemical parameters of domestic and miniature donkeys (Equus africanus asinus). Ten clinically healthy donkeys were selected for this study. The overall study population was almost equally subdivided into six subgroups according to the breed (domestics and miniatures), ...
Source: Comparative Clinical Pathology - February 28, 2017 Category: Pathology Source Type: research

The effect of detomidine on clinical signs, serum electrolytes, electrocardiographic indices, and cardiac arrhythmias in Camelus dromedarius
AbstractThe aim of this study was to document sedation and analgesic effects, electrocardiographic (ECG) parameters, and some physiologic variable and serum concentrations of electrolytes after intravenous (IV) administration of detomidine in camels. Five healthy female dromedary camels (Camelus dromedarius) aged 2  years and average weight of 200 ± 20 kg were used. Data were recorded before and 3, 5, 10, 15, 30, 45, 60, and 90 min after IV administration of detomidine (50 μg kg−1). Sedation and antinociception response were recorded. The ECG was attached as a bipolar base...
Source: Comparative Clinical Pathology - February 27, 2017 Category: Pathology Source Type: research

Plasma amylase and lipase concentrations in captive black-tailed prairie dogs ( Cynomys ludovicianus )
This study was conducted in order to determine plasma amylase and lipase concentrations in captive black-tailed prairie dogs (Cynomys ludovicianus). Twenty-six healthy intact captive prairie dogs of both sexes were studied as part of an overall clinical health evaluation performed under general isoflurane anesthesia. Each animal underwent a complete physical examination, complete blood count, plasma biochemistry, and venous blood gas analysis. Venous blood samples were placed in heparinized blood-collection tubes and submitted for plasma amylase and lipase concentration analyses. The reference interval for amylase was 111....
Source: Comparative Clinical Pathology - February 27, 2017 Category: Pathology Source Type: research

Glutathione is a novel treatment for cystine stones
AbstractThe present study evaluates and compares firstly the serum biomarkers of oxidative stress, superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), malondialdehyde (MDA), glutathione oxidized and reduced, homocysteine (Hcy), and total antioxidant capacity (TAOC), and secondly computed tomography (CT) scans for 30 diagnosed cystinuric patients before and after taking glutathione or placebo. The levels of SOD were significantly higher in patients who took glutathione (0.25  ± 0.10 ng/ml) when compared with patients who took placebo (0.17 ± 0.07 ng/ml,p value 0.047)...
Source: Comparative Clinical Pathology - February 25, 2017 Category: Pathology Source Type: research

A glutamine and glutamate mixture and its effects on the hematological and biochemical biomarkers in dogs
In conclusion, supplementation with a mixture of glutamine and glutamate was able to raise the concentration of these amino acids in the blood of healthy dogs, without changes in blood protein biomarkers and any adverse reaction. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 24, 2017 Category: Pathology Source Type: research

Haematopoietic induction and hepatic protective roles of Hepacare ® in CCl 4 -induced hepatic damaged rats
AbstractHerbal formulations are plant parts used as raw materials for self-administered pharmaceutical remedies, and many of them are being sold without any scientific validation for their potency and efficacy. This research work was aimed at evaluating the haematopoietic, biochemical, and histological effects of Hepacare ®, a popularly sold herbal formulation in Nigeria against carbon tetrachloride (CCl4)-mediated liver damage in rats. Haematological analysis showed significant reduction (p 
Source: Comparative Clinical Pathology - February 24, 2017 Category: Pathology Source Type: research

Suppression of phagocytic oxidative burst, cytotoxic effect, and computational prediction of oral toxicity of dietary fatty acids of Clerodendrum volubile stem
AbstractOil and fatty acids from the flowers and stems ofClerodendrum volubile were investigated for their antioxidative burst activities. The oils were methylated to obtain fatty acid methyl esters (FAMEs). The oils and fatty acids were then subjected to GC-MS analysis to identify the compounds present. Their inhibitory potentials on oxidative burst were investigated on blood phagocytes using the luminol-amplified chemiluminescence technique after activation with serum opsonized zymosan. Succinate derivatives were observed to be the most predominant compounds.C. volubile stem fatty acids showed potent inhibitory activity ...
Source: Comparative Clinical Pathology - February 23, 2017 Category: Pathology Source Type: research

Effects of preanesthetic administration of metamizole on renal function, blood parameters and bone marrow cells in healthy dogs
This study evaluated the systemic effects of metamizole with or without general anesthesia in healthy dogs. Twelve mixed healthy dogs of either sex were divided randomly into two groups (control and anesthesia groups). Following a 12-h period of fasting (food and water), both groups received the first dose of metamizole (35  mg/kg) intravenously and then 4 doses intramuscularly every 12 h after receiving the first dose of metamizole. In the anesthesia group, the animals underwent halothane inhalation anesthesia for 2 h after receiving the first dose of metamizole. The vital signs were monitored but animals d...
Source: Comparative Clinical Pathology - February 23, 2017 Category: Pathology Source Type: research

Study on the renal development (morphological and stereological) in H. huso ( Beluga sturgeon ) larvae
AbstractThe aim of this study was to obtain the information about kidney evolution inHuso huso. According to the results on the 1st (day of post hatching (dph)), the pronephros with collecting canal and hemato-poietic tissue was distinguished. On the 3rd (dph), the primordial mesonephric tubules, rounded cell clusters, and a primary coelom cavity were observed. On the 6th (dph), the pronephros was gradually degenerated and changed to mesonephros. On the 15th (dph), the collecting canal was resembled as a small canal, and three parts, head, body, and caudal, could be distinguished in the kidney. The tubules were differentia...
Source: Comparative Clinical Pathology - February 23, 2017 Category: Pathology Source Type: research

Evaluation of changes in wound assessment parameters associated with contra-lateral spermatic cord as ligature in open and closed pre-scrotal and scrotal castrations
This study evaluated changes in wound assessment parameters associated with contra-lateral spermatic cord as ligature in open and closed pre-scrotal and scrotal castrations in growing Nigerian male mongrels. Sixty client-owned dogs were randomly assigned into 6 groups (n=10). The spermatic cord was used as ligature in 4 groups (1, 2, 5, 6) while chromic catgut ligature was used in the other 2 groups (3 and 4). Groups 1, 3 and 5 were pre-scrotally castrated while groups 2, 4 and 6 were scrotally castrated using standard methods. Incisions were closed in groups 1, 2, 3 and 4 while in groups 5 and 6, incisions remained open. ...
Source: Comparative Clinical Pathology - February 22, 2017 Category: Pathology Source Type: research

Altered blood oxidative stress biomarkers in association with canine pyoderma and allergic contact dermatitis
This study was carried out on 25 dogs, in which ten dogs were used as controls, nine dogs were affected with pyoderma, and six dogs had ACD. Analysis of oxidative stress markers showed a significant decrease of the mean values of GPx and CAT enzymes in pyoderma-infected dogs while showing a significant increase of the mean value of SOD enzymes along with a decrease of the plasma zinc level in ACD-affected dogs. Pyoderma and ACD are associated with significant changes in plasma biomarkers of oxidative stress. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 21, 2017 Category: Pathology Source Type: research

Detection of equine leptospiral antibodies by latex agglutination test in Iran
AbstractLeptospirosis is a global zoonotic disease caused by someLeptospira species but relatively mild in the horse except for blindness due to recurrent uvitis. The aim of the current investigation was to detect equine leptospiral antibodies using the latex agglutination test in Golestan, Kerman, and Yazd provinces, Iran. A total of 180 serum samples were collected from clinically healthy horses at nine race clubs in Golestan, Yazd, and Kerman provinces, Iran, and evaluated by the latex agglutination test commercial kit. Based on the results, antibodies were detected in 44 sera (24.44%) among 180 samples. It is recommend...
Source: Comparative Clinical Pathology - February 21, 2017 Category: Pathology Source Type: research

Molecular evidence of Anaplasma phagocytophilum : an emerging tick-borne pathogen in domesticated small ruminant of Iran; first report
AbstractRickettsia from the genusAnaplasma is obligated intracellular microorganism of animal host and human. The aim of this study was to investigate the prevalence and distribution ofAnaplasma phagocytophilum in domesticated small ruminant of Iran. Three hundred seventy blood samples of sheep and goat were collected from different regions in Hamedan province, Iran. The DNA was extracted from blood samples and subsequently, 16S rRNA and MSP4 genes were analyzed by Nested-PCR method and overall 1.08% (4/370) of domesticated small ruminants were positive forA.phagocytophilum infection. The data recorded were analyzed by the...
Source: Comparative Clinical Pathology - February 20, 2017 Category: Pathology Source Type: research

Risk factors for Toxoplasma gondii in sheep of southern Brazil
AbstractToxoplasma gondii is responsible for reproductive failure in sheep, and it is considered a zoonotic parasite. Therefore, the aim of this study was to evaluate the prevalence of toxoplasmosis and its risk factors at the farm level. For that, 314 sheep from 18 farms of 7 municipalities of the West part of Santa Catarina State, Southern Brazil were used. To identify possible risk factors associated to the disease, a survey was applied to farmers with questions regarding farm characteristics, type of management, and history of animal reproductive problems. Out of 314 sera samples, 118 (37%, CI 95% 32.3 –43.3) wer...
Source: Comparative Clinical Pathology - February 19, 2017 Category: Pathology Source Type: research

Fish oil protects rat erythrocytes against oxidative stress caused by Trypanosoma evansi infection
AbstractThe effect of omega-3 polyunsaturated fatty acids has been the subject of intense study in many diseases; meanwhile, our understanding regarding the underlying mechanisms of omega-3 fatty acids againstTrypanosoma evansi infection is still limited. A common feature ofT. evansi infection is anemia. Erythrophagocytosis occurs due to direct traumatic actions of protozoa on the red blood cells, but the exact mechanisms of anemia are not yet fully understood. During infection, there is an increase in reactive oxygen species; some are highly toxic and rapidly detoxified by various cellular enzymatic and nonenzymatic mecha...
Source: Comparative Clinical Pathology - February 18, 2017 Category: Pathology Source Type: research

Blood chemistry and haematology of West African Dwarf goats fed Moringa oleifera leaf meal (MOLM) in their diet
AbstractWest African Dwarf (WAD) goat is one of the most prolific goat species in Nigeria, and its production fulfils important socio-economic functions. In Nigeria, high cost of feed and inadequate nutrient intake undermines this goat breed in expressing their full potential. Therefore, the effect of 90  days of feeding MOLM diets on blood chemistry and haematology of WAD goats were investigated. Thirty six, 10–11-months old WAD goats were divided into four groups of nine , and each group was replicated thrice. Each group was assigned to one of the experimental diets in a completely randomized d esign tagged A ...
Source: Comparative Clinical Pathology - February 17, 2017 Category: Pathology Source Type: research

The effect of castration on cortisol changes and blood glucose in horses
In this study, cortisol concentration was measured after a surgery in order to determine the relationship between horse ’s castration, adrenal gland excretion, and level of stress. Twelve horses were selected for surgical castration. Scrotal incision and closed approach technique were performed for both testicles. Blood was taken before surgery, 10 and 30 days after surgery (castration). Blood glucose was measured by glucose oxidase and then serum and plasma hormones cortisol were measured. Finally, Paired samplet test was performed for statistical analysis using SPSS 16 software (SPSS Inc., Chicago, IL, USA). N...
Source: Comparative Clinical Pathology - February 16, 2017 Category: Pathology Source Type: research

Evaluation of wound healing activity of St. John ’s Wort ( Hypericum perfoliatum ) in horses
This study has certainly given a scientific point of view to a century of empiricism about the use of SJW in veterinary practice. The findings of this study seem to suggest that SJW may be considered an “all-in-one” remedy for wound healing, in order to make the management of skin injuries easier even for severe and complicated lesions. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 15, 2017 Category: Pathology Source Type: research

Blood chemistry and haematology of finisher broilers fed with plantain ( Musa paradisiaca L) peel in their diets
AbstractA 4-week study was carried out to determine the effects of partial replacement of maize in broiler finisher diets with plantain peel on their blood chemistry and haematology. One hundred and twenty-six (126), 4-week-old Marshall Broilers weighing 1.0  kg were divided into 3 groups of 42 birds. Each group was replicated 3 times with 14 broiler chicks per replicate and each replicate housed in a pen measuring 1.5 m × 2.0 m. Each group was assigned to one experimental diet in a completely randomized design designated T1 (0%), T2 (10%), and T3 (20%). The condition of housing and managemen...
Source: Comparative Clinical Pathology - February 13, 2017 Category: Pathology Source Type: research

A comparative study on selected APP, alkaline phosphatase and lactate dehydrogenase activities in buffalo and cow with subclinical mastitis
AbstractThe aim of this study was to evaluate the comparative changes of selected acute-phase proteins (APPs), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activities in buffalos and cows with subclinical mastitis. For this purpose, a total of 139 buffalos (Bubalus bubalis) and 146 cows (Holstein or Friesian) from Dakahlia governorate in Egypt were studied. Seventy-six cows and 49 buffalos exhibited subclinical mastitis with positive California mastitis test (CMT) and no clinical signs of mastitis. Results revealed that the most dominant isolated bacteria wereStaphylococcus spp. in both buffalo and cow fello...
Source: Comparative Clinical Pathology - February 13, 2017 Category: Pathology Source Type: research

Effect of salbutamol against the experimental acute colitis in rat model
In conclusion, salbutamol can ameliorate acute ulcerative colitis, possibly via the stimulation of beta2 receptors. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 11, 2017 Category: Pathology Source Type: research

Prevalence and economic importance of Coenurus Cerebralis in sheep and goats in and around “Legahida” district of bale zone, south eastern Ethiopia
AbstractCoenurosis is a disease caused byCoenurus cerebralis, the larval stage ofTaenia multiceps, particularly affecting sheep and goats.T. serialis andT. brauni could also cause coenurosis. The final hosts are domestic and wild canidae. Cross sectional study was conducted from November 2014 to March 2015 to estimate the prevalence and associated risk factors ofCoenurus cerebralis in both sheep and goats in Legahida backyard slabs. The estimation of its economic burden and assessment of knowledge, attitude, and practices (KAP) of the pastoral and agro-pastoral communities were also performed for which questionnaire survey...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Cholinesterase ’s activities in cows supplemented with selenium, copper, phosphorus, potassium, and magnesium intramuscularly during the transition period
AbstractThe cholinesterases are enzymes involved in severe biological functions in mammals; however, it was not yet study in dairy cows during the transition period. In this period, the animals have high energetic demands for milk production, and the supplementation with mineral can be considered an important approach to fulfill this demand. Thus, the aim of this study was to evaluate the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in dairy cows supplemented with minerals based on selenium, copper, phosphorus, potassium, and magnesium intramuscularly in the transition period. The mineral supp...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Morphological features of glands in the gastrointestinal tract of the African pied crow ( Corvus albus )
In conclusion, the data presented in this study represent morphofunctional modifications of the gut of the crow that may be related to their feeding habit, exposure to microorganisms, and species adaptations which could guide the management of this wild species. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Serum biochemical changes following constant rate infusion of Ketoprofen and Ketoprofen + Tramadol combination in laparotomized dogs
AbstractThere is no data on safety of ketoprofen and ketoprofen  + tramadol combination by constant rate infusion for the management of laparotomy-induced pain. The study investigated the serum biochemical changes associated with the use of ketoprofen and ketoprofen + tramadol combination by constant rate infusion in laparotomized dogs. Fifteen adult female Nigerian mongrels were randomly assigned into three groups (n = 5). Group 1 was treated with normal saline; group 2 received 1.0 mg/kg/h K while group 3 received KT at 0.5 mg/kg/h each. Ventral midline was performed on all do...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Toxicity and nutritional studies on Mucuna pruriens leaves from Nsukka, South-Eastern Nigeria
AbstractNutrient, proximate and phytochemical analyses ofMucuna pruriens leaves were done. Acute and sub-acute toxicity studies on the methanol extract were done. Eighteen rats dosed with the extract at 10, 100, 1000, 1600, 2900 and 5000  mg/kg body weights were used for acute toxicity test. For the sub-acute toxicity study, 24 rats randomly allotted into four groups (A–D) of six animals each were used. Graded doses (100, 200, 400 mg/kg) of the extract were administered orally to groups A, B and C, respectively, for 28 days. Gr oup D was the untreated control. Body weight changes, gross and histopathol...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Cytotoxic activity of fumonisin B 1 in Vero cells: comparison between 2D and 3D structural microplates
AbstractThe present study aimed to estimate cytotoxic activity of fumonisin B1 (FB1) in Vero cells using crystal violet staining assay (CVA) and lactate dehydrogenase assay (LDH) performed in flattened (2D) and three dimensional (3D) bottom (V, U, and M) microplates. Vero cells were exposed to different concentrations of FB1 ranging from 0 to 10  μg/ml for 24 and 48 h. Tween 20 was used as a positive control and acetonitrile 50% in water was used as a negative control. Results revealed that CVA could be performed in the F type microplate for 24 and 48 h but it was useless in the 3D microplates. LDH assay ...
Source: Comparative Clinical Pathology - February 4, 2017 Category: Pathology Source Type: research

Subchronic toxicity studies of aqueous leaf and stem bark extract of Irvingia gabonensis in male albino wistar rats
This study investigated the effect of aqueous leaf and stem bark extracts ofI. gabonensis on the haematology, pathomorphology and some clinical chemistry profiles of male albino wistar rats following subchronic exposure. The extract was administered using the oral route. Group A served as control and was given only distilled water. Groups B, C and D were treated with the extract at 100, 1000 and 2000  mg/kg, respectively. The parameters assessed to determine the effect of subchronic administration of the extract were: packed cell volume (PCV), haemoglobin concentration (Hb), total red blood cell count (TRBC), total wh...
Source: Comparative Clinical Pathology - February 3, 2017 Category: Pathology Source Type: research

Examining responses of chicken embryonic neural stem cell to infectious laryngotracheitis virus infections
AbstractInfectious laryngotracheitis virus (ILTV) infection causes economic losses in the poultry industry. Outbreaks of the infection in commercial poultry are controlled by using attenuated live vaccines. The genomic and antigenic characteristics between virulent and vaccine strains of ILTV are very similar, thus the differential diagnosis of ILTV strains is targeted in controlling programs. Here, the chicken embryonic neural stem (cENS) cell serves as a host model to study virulence characteristics of ILTVs. The susceptibility of cENS cells to different ILTV strains infection was investigated by analyzing the impact of ...
Source: Comparative Clinical Pathology - January 27, 2017 Category: Pathology Source Type: research

Genetic influence of DNAM-1 (DNAX accessory molecule-1) gene polymorphism (rs 763361) on susceptibility and disease activity of systemic lupus erythematosus and rheumatoid arthritis in Egyptian patients
This study aimed to determine the possible genetic influence of SNP on disease activity in SLE (represented by SLEDAI score) and in RA patients (represented by DAS28). Fifty SLE patients compared to 43 healthy controls and 35 RA patients compared to 30 healthy controls were included in the study. The PCR-RFLP technique was used to detect DNAM-1(C/T rs 763361) gene polymorphism. Analysis of the frequency distribution of both allele and genotype frequency distribution of SLE and RA patients compared to healthy control subjects did not reveal a significant association between SLE and RA patients and the rs763361. Analysis of ...
Source: Comparative Clinical Pathology - January 26, 2017 Category: Pathology Source Type: research

Use of homeopathic product to prevent ketosis in the dairy sheep during the transition period
AbstractPregnancy toxemia is a severe problem in sheep in the transition period, which is most likely related to feeding. There is a need to seek alternatives to this problem, and the homeopathic industry has invested in products for this purpose. The product (Figotonus ®) tested in this work was added to the diet of dairy sheep at different dosages (1, 5, and 10 g/day/animal) to verify the effects on animal health indicators, focusing mainly on the reduction of cases of ketosis. In all, 32 Lacaune ewes were used, with confirmed pregnancy presenting two to six bi rths during their lifetime. The following blood bio...
Source: Comparative Clinical Pathology - January 25, 2017 Category: Pathology Source Type: research

Evaluation of salivary level of IL-10 in patients with oral lichen planus, a preliminary investigation
AbstractOral lichen planus is an inflammatory disorder with T-cell-mediated immunological etiology. The aim of this study was to evaluate the IL-10 in saliva of OLP patients and compare it with healthy people. In this cross-sectional study, 44 patients with oral lichen planus and 44 healthy people were enrolled to the study. Five to 10  ml un-stimulated whole saliva samples were collected in the morning, and concentration of IL-10 was measured using enzyme-linked immunosorbent assay (ELISA). The mean of salivary IL-10 was more in the patient group (0.2 ± 0.92) than the healthy group (0.1 ±...
Source: Comparative Clinical Pathology - January 25, 2017 Category: Pathology Source Type: research

Histopathological study in wild freshwater stingrays Potamotrygon motoro in the Peruvian Amazon
The objective of the present study was to investigate the occurrence of histopathological alterations in some tissues of the Amazonian freshwater stingrayPotamotrygon motoro. Thirty-one specimens were collected from the Amazon basin and transported to a commercial aquarium in district of Juan Bautista, province of Maynas, department of Loreto, Peru. The histopathological study showed monogeneans in gills (2/31),Piscinoodinium pillulare in gills and skin (2/31), digenean metacercariae in liver (3/31), and coccidia in the lumen and mucosa of the spiral valve (2/31). In addition, there were found non-infectious lesions in the...
Source: Comparative Clinical Pathology - January 24, 2017 Category: Pathology Source Type: research

Immunohistochemical expression of transforming growth factor Beta-1 in canine mammary carcinomas: its relationships with histologic grading, survival rate, and recurrence
In this study we aimed to investigate Transforming Growth Factor Beta-1 (TGF- β1) expression in canine mammary carcinomas (CMCs) by immunohistochemistry and its relationships with histologic grading, survival rate, and recurrence. Tissues were selected from 35 cases of CMCs and were included in a 2-year follow-up study. Immunohistochemistry for detection of TGF-β1 protein w as carried out using Avidin-Biotin Complex (ABC) method, and the immunostained slides were scored according to the distribution and intensity of positive reactions. Results indicated that TGF β-1 expression increased significantly in CMCs...
Source: Comparative Clinical Pathology - January 21, 2017 Category: Pathology Source Type: research

Two foals with ectrodactyly in hindlimb: first report
AbstractEctrodactyly refers to the congenital absence of phalanges as total or partial. In the veterinary literature, ectrodactyly has been described in dogs and domestic cats as a rare congenital heterogeneous disorder. The current report has described unilateral ectrodactyly in the left hindlimb of two born foals in an area with presence of Iranit and zinc manufactures. One of the foals was lack of the third phalanx and the other one had not the third and second phalanxes. No other abnormality was found in these cases. Various teratogens may be responsible for occurring skeletal anomalies. To the best of our knowledge, t...
Source: Comparative Clinical Pathology - January 21, 2017 Category: Pathology Source Type: research

A comparative analysis of the molecular basis of fibrosis between tissues
This article discusses key molecular mechanisms of fibrosis within tissues and highlights the similarities and differences amongst key pathways. There is compelling evidence for the involvement of toll-like receptor-4 and transforming growth factor β within fibrosis throughoutall tissue types. With regards to other molecules, although there are similarities between tissues, evidence is inconsistent. In order to gain therapeutic advances in the prevention or treatment of fibrosis, research should strive to understand specific molecular mechanisms in models that closely replicate human disease. Further, there should be ...
Source: Comparative Clinical Pathology - January 20, 2017 Category: Pathology Source Type: research

Erratum to: Evaluation of the healing effect of honey and colostrum in treatment of cutaneous wound in rat
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 20, 2017 Category: Pathology Source Type: research

Pathological and biochemical studies on enterotoxemia in sheep
In conclusion, enterotoxemia as a fatal enteric disease is associated with some characteristic clinical, biochemical, bacteriological, and histopathological alterations. These alterations can be a useful aid for rapid diagnosis of enterotoxemia in sheep farms. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 19, 2017 Category: Pathology Source Type: research

Overexpression of the giant FAT1 cadherin gene and its prognostic significance in de novo acute leukaemia patients
AbstractFAT1 is a type 1 transmembrane protein belonging to the cadherin superfamily. Many reports showed either overexpression of FAT1 gene or its loss in solid cancers. Limited studies evaluated its importance in leukemogenesis. Real-time quantitative reverse transcriptase polymerase chain reaction (RTQ-PCR) was used to examine the levels of FAT1 mRNA expression in 50 paediatric acute lymphoblastic leukaemia (ALL) and 50 adult cytogenetically normal acute myeloid leukaemia (CN-AML). Each group was compared with 20 age- and gender-matched healthy controls. Peripheral blood (PB) samples were collected at time of diagnosis ...
Source: Comparative Clinical Pathology - January 17, 2017 Category: Pathology Source Type: research

Anti-diabetic activity of Garcinia xanthochymus seeds
AbstractIn the present study, extracts ofGarcinia xanthochymus seeds were used to determine anti-diabetic, anti-oxidant, and biochemical activities in alloxan-induced diabetic mice. Of the petroleum ether (G. xanthochymus petroleum ether extract (GXSPE)) and methanol (G. xanthochymus methanol extract (GXSME)) seed extracts, 100 and 200  mg/kg were used and compared with normal, diabetic, and glibenclamide groups for various activities. The parameters like blood glucose, body weight, serum biochemicals, serum enzymes, and anti-oxidative enzymes in different groups were monitored at regular intervals. The extracts produ...
Source: Comparative Clinical Pathology - January 16, 2017 Category: Pathology Source Type: research

Strong expression of interleukin-17 is associated with higher histologic grades in canine mammary carcinoma
This study investigates interleukin-17 expression in canine mammary tumours by immunohistochemistry and its relationships with histologic grades, tumour recurrence and survival after 2  years. Results showed that 23 samples (63.88%) showed strong immunoreactivity for interleukin-17 and 13 samples (36.11%) showed moderate immunoreactivity. Interleukin-17 expression is significantly increased in canine mammary tumours compared to normal canine mammary tissues (P 
Source: Comparative Clinical Pathology - January 16, 2017 Category: Pathology Source Type: research