Experimental evaluation of an antimicrobial protein from Bacillus amyloliquefaciens MBL27 for wound healing potential in rats
This study was aimed at assessing the ability
of the antimicrobial protein (AMP) produced byBacillus amyloliquefaciens MBL27 as a potent wound healant. Rat
models were used to study the efficacy of AMP and AMP-incorporated chitosan
sheet along with control groups. AMP and AMP-incorporated chitosan sheet
significantly improved wound contraction when compared to controls. Rate of
wound contraction (97.23%), decreased period of epithelialization (14 days),
and the levels of biochemical markers such as hydroxyproline (collagen), total
protein, uronic acid, and hexosamine in the granulation tissue on differ...
Source: Comparative Clinical Pathology - May 25, 2022 Category: Pathology Source Type: research
The impacts of dosage and route of administration of Methotrexate on induction intestinal mucositis in rats: a comparative pathological study
AbstractMethotrexate (MTX)-induced intestinal mucositis is one of the most frequent and expeditious animal models of mucositis. However, multiple methods of MTX dosing exist in the literature. Hence, to improve the reliability and utility of this model, the present study aimed to compare multiple schedules of MTX dosing for the development of intestinal mucositis in rats. A total of 56 male Wistar rats (8-week-old) were randomly allocated into seven equal groups. The control group was treated with saline intraperitoneally (IP), and experimental groups received MTX at five different single doses of 15 mg/kg (MTX15), 20 m...
Source: Comparative Clinical Pathology - May 23, 2022 Category: Pathology Source Type: research
Hirudin as an anticoagulant in hematology and plasma biochemistry of dog: comparison with EDTA and serum
AbstractEven though the effect of various anticoagulants on hematological and biochemical parameters of human and different animals ’ blood have been studied in the past but, there is limited information on the effects of hirudin as an anticoagulant substance in dog clinical pathology. In the current study, we evaluated the efficacy of hirudin as an anticoagulant agent compared to EDTA and serum. Twenty adult dogs (ranging fro m 2 to 6 years old and 25 ± 3 kg) were chosen, and blood samples were taken from the jugular vein and then were added to three test tubes containing anticoagulant substances and one test tub...
Source: Comparative Clinical Pathology - May 18, 2022 Category: Pathology Source Type: research
Phytochemical screening and toxicity investigation of hydro-methanolic and aqueous extracts from aerial parts of Sonchus oleraceus L. in Swiss albino mice
AbstractSonchus oleraceus L. is a Mediterranean leafy vegetable. The aerial parts are widely used in Algerian traditional medicine to treat several diseases such as inflammation, hepatitis, and for detoxication. To date, there are no studies corroborating its toxicity profile. This work aimed to investigate the qualitative phytochemical screening (QPS) and to evaluate the toxicity of the hydro-methanolic (HME) and hot aqueous (HAE) extracts of the aerial parts ofS. oleraceus L. in female mice. The QPS of both extracts was achieved using simple tests, as well as the acute and subacute toxicities were examined following Lork...
Source: Comparative Clinical Pathology - May 11, 2022 Category: Pathology Source Type: research
Hyponatraemia and hypokalaemia relationship with alterations of glucose, cholesterol and total protein levels during human infection with Plasmodium falciparum
AbstractElectrolytes, particularly sodium and potassium, are highly paramount for the uptake of nutrients that are required for the proliferation, differentiation and survival ofPlasmodium falciparum in the host. Sequel to this essential role, information on the interplay between electrolytes and nutrients would add to the current understanding of the malarial infection pathophysiology. To this end, we carried out a hospital-based cross-sectional study and estimated the serum levels of sodium, potassium, glucose, cholesterol and total protein in bothP. falciparum-infected patients and apparently healthy patients. Our resul...
Source: Comparative Clinical Pathology - May 7, 2022 Category: Pathology Source Type: research
Diagnostic value of procalcitonin, C-reactive protein, and leukocyte count in canine ehrlichiosis and canine demodicosis
AbstractCanine ehrlichiosis (E. canis) is an obligate intracellular organism that resides as a microcolony within a membrane-lined intracellular vacuole (morulae), primarily within monocytes and macrophages of dogs. Canine demodicosis (D. canis) is an inflammatory parasitic skin disease associated with the presence of excessive numbers ofDemodex mites. We aimed to conduct a comprehensive study on blood procalcitonin (PCT), C-reactive protein (CRP), leukocytes count alterations along with hemato-biochemical, and the clinical observations including clinical history and physical examination in dogs infected with canine ehrlic...
Source: Comparative Clinical Pathology - May 6, 2022 Category: Pathology Source Type: research
Effects of rosiglitazone and vitamin E on testicular tissue and sperm parameters in propylthiouracil-induced hypothyroidism in rats
AbstractThyroid dysfunction has been reported to negatively affect the male reproductive system. A clear link exists between thyroid hormone (TH) and testicular development and function. Here, the effects of one of the peroxisome proliferator-activated receptor-gamma agonists, rosiglitazone (RSG), and vitamin E (Vit E) on testicular histology in propylthiouracil (PTU)-induced hypothyroidism were investigated. Thirty-five juvenile male Wistar rats were included in 5 groups: control group, hypothyroid group which received 0.05% PTU in drinking water, and rats in groups 3 and 4 besides PTU, treated with 4 mg/kg RSG and 20 ...
Source: Comparative Clinical Pathology - April 30, 2022 Category: Pathology Source Type: research
Serosurvey for Middle East respiratory syndrome coronavirus antibody in dromedary camels and human patients at a secondary care hospital, Illela, Northwest Nigeria
This study serves as a baseline for similar researches and due to the high seroprevalence obtained in this study for both camels and humans, there is need for trained personnel, surveillance and diagnostic tools at our border posts and animal markets. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 28, 2022 Category: Pathology Source Type: research
Possible hepatoprotective potentials of Dialium guineense pulp on acetaminophen-provoked hepatotoxicity in female albino rats
In conclusion,Dialium guineense extract exhibited possible hepatoprotective and in vivo antioxidant activities especially at the inclusion rate of 6.25%. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 20, 2022 Category: Pathology Source Type: research
Morphofunctional state of the salivary glands of Wistar rats under prolonged exposure to alcohol
In this study, sexually mature male albino Wistar rats consumed 20% ethanol (6.9 g/kg/day) for 180 consecutive days. PGs and SMGs were collected for morphometric and luminescence-histochemical analyses (nonparametric Mann–WhitneyU-test,p < 0.05). PGs exposed to ethanol for 180 days showed a change in the shape of the acini and secretory cells that formed them, uneven expansion of the interlobular excretory ducts, and moderate fatty infiltration of the stroma. SMGs exposed to ethanol for 180 days showed changes in acinar cells, in tercalated and striated ducts, fatty infiltration, mild atrophy, and stromal ede...
Source: Comparative Clinical Pathology - April 19, 2022 Category: Pathology Source Type: research
Oxidative stress induced by Roundup in rat liver and brain: attenuation by zinc sulfate
In conclusion, this study indicates that Zn protects against the toxicity of Roundup by providing hepatoprotective and neuroprotective effects. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 19, 2022 Category: Pathology Source Type: research
Effects of different extracts of Azanza garckeana fruit pulp on haematological and biochemical parameters of New Zealand White (NZW) rabbit bucks
AbstractHaematological and biochemical parameters are used to determine the extent of deleterious effects of xenobiotics, including plant extracts, on animals. The aim of this study was to investigate the effects of different extracts ofAzanza garckeana (AG) fruit pulp on haematological and serum biochemical parameters in rabbit bucks. Eighteen (18) New Zealand White rabbit bucks with average live weight of 1.2 kg and aged 8–16 months were fed ad libitum on a commercial isocaloric and isonitrogeneous diet. They were administered different extracts of AG at a dose of 500 mg/kg weight via oral gavage. The extracts comp...
Source: Comparative Clinical Pathology - April 18, 2022 Category: Pathology Source Type: research
Protocatechuic acid through modulation of signaling pathways and oxidative stress exerts protective effects in rat model of carbon tetrachloride-induced renal and reproductive toxicities
This study examines modulatory effect of PCA against carbon tetrachloride (CCl4)-induced toxicities on the kidney and testes of rats. Rats were treated as follows: group 1 (olive oil), group 2 was given CCl4 only intraperitoneally at 3 ml/kg in a suspension of olive oil, groups 3 and 4 were pretreated with PCA orally at 10 and 20 mg/kg respectively for 7 days before CCl4 challenge. Biochemical investigations were carried out by assessing serum biochemical markers, testicular hormones levels and sperm parameters, oxidative stress markers, expression levels of NF-kB, COX2 and TNF, antioxidant enzymes activities, and hist...
Source: Comparative Clinical Pathology - April 18, 2022 Category: Pathology Source Type: research
Phlogacanthus thyrsiflorus Nees. modulates hepatic and renal apoptosis via attenuation of oxidative stress in alloxan-administered mice
AbstractAn endeavor has been made in this study to assess the effect ofPhlogacanthus thyrsiflorus on hyperglycemia-induced oxidative stress along with apoptosis in liver and kidney of alloxan-administered mice. To analyze the effect ofPhlogacanthus thyrsiflorus on oxidative stress in kidney and liver of diabetic mice. Furthermore, we have also examined the effect ofPhlogacanthus thyrsiflorus on apoptosis in diabetic mice. Following preparation of methanolic flower extract (MFE), preliminary phytochemical screenings and acute toxicity test were carried out for MFE. Lipid peroxidation and protein carbonyl assays were d...
Source: Comparative Clinical Pathology - April 18, 2022 Category: Pathology Source Type: research
Insights into the mechanism underlying reproductive toxicity of gasoline fumes in male albino rat
AbstractFew studies have reported reproductive toxicity of gasoline fumes in animals, but none of the findings has been fully ascertained and the mechanism underlying the observed toxicity still remains unknown. Here we present insights into the potential mechanism underlying the reproductive toxicity of gasoline fumes in 72 adult male albino rats randomly assigned to six groups of 12 rats per group. Rats in group 1 (control) were exposed to distilled water while those in groups 2, 3, 4, 5, and 6 were daily exposed to gasoline fumes for 1, 3, 5, 7, and 9 h respectively for 10 weeks. Serum level of reproductive hormones ...
Source: Comparative Clinical Pathology - April 18, 2022 Category: Pathology Source Type: research
