Inhibitory effects of lactic acid bacteria isolated from traditional fermented foods against aflatoxigenic Aspergillus spp.
AbstractIn the present study, after molecular identification of dominant lactic acid bacteria (LAB) isolated from traditional fermented foods, antifungal activity of the isolates was investigated against aflatoxigenicAspergillus spp. Based on screening results, among the isolated LAB,Pediococus lolii had the highest inhibitory effect againstAspergillus flavus (70.32%) andAspergillus niger (98.8%). Furthermore, antifungal activity ofP. lolii stationary phase cell-free supernatant (CFS) was significantly (P 
Source: Comparative Clinical Pathology - May 27, 2017 Category: Pathology Source Type: research

Biochemical evaluation of amniotic and allantoic fluids of the Red Sokoto goat in the second term of pregnancy —a preliminary study
This study evaluated the biochemical profile of the allantoic and amniotic fluids of pregnant Red Sokoto goats in their second term of pregnancy. Aliquots of each of the allantoic and amniotic fluids were aspirated and placed into separate clean sample bottles. These foetal membrane fluids samples were preserved at −20° and used for the biochemical evaluations. The foetuses were exteriorized, and the sexes of the foetuses were noted, while their crown-rump lengths (CRL) were measured. The CRL was used for gestational age determination. Allantoic and amniotic fluids from does of gestational age between 50 an d 100...
Source: Comparative Clinical Pathology - May 27, 2017 Category: Pathology Source Type: research

Protective effects of royal jelly on the histomorphologic, oxidative stress and sperm parameters in Ofloxacin treated rat
AbstractRoyal jelly is produced by worker bees as nutrition for bee larvae and adult queens and has also been shown to have protective effects against antibiotics. The aim of this investigation was to determine protective effect of royal jelly on the reproductive functions of male rats treated with ofloxacin. In this experiment, 32 mature male albino rats were randomly allocated into four groups (n = 8): control, ofloxacin only, royal jelly only, and ofloxacin with royal jelly. The results revealed that ofloxacin alone caused significant decreases (P 
Source: Comparative Clinical Pathology - May 27, 2017 Category: Pathology Source Type: research

The central effect of 3-iodothyronamine on brain neuropeptides in mice
In this study, we attempted to find out whether chronic low dose 3-iodothyronamine treatment could modulate some food intake regulatory neuropeptides such as leptin, ghrelin, and galanin in mice brain. Eighteen male mice were divided randomly into control (n = 8) and treatment (n = 10) groups. The experimental procedure was applied for 7 days during which treatment group received T1AM (i.p) whereas the control group received DMSO and normal saline. The brain was analyzed for leptin, ghrelin, and galanin concentrations. There were significant differences in leptin concent ration (1.75 ±&...
Source: Comparative Clinical Pathology - May 24, 2017 Category: Pathology Source Type: research

Ultrasonographic and histopathologic changes following injection of neutral zinc gluconate in dog ’s ovaries
In this study, four female healthy and fertile dogs were selected randomly. Under general anesthesia and during laparotomy, the neutral sterile zinc gluconate was injected directly into the ovaries of five dogs. Changes in the size and appearance of the ovaries, leukocytes , and vaginal cytology were monitored before and after the start of the study twice a week. After 1 month, the uterus and ovaries were removed surgically and sent to laboratory for histopathologic examination. Except one dog which was in diestrus, the other dogs were in anestrus during the study. B ased on ultrasonographic examination, zinc gluconat...
Source: Comparative Clinical Pathology - May 22, 2017 Category: Pathology Source Type: research

Emphysematous lymphangitis in the colon of a steer
AbstractEmphysematous lymphangitis was found in the colon of a 2-year-old Holstein-Friesian steer which was conventionally slaughtered for food. At postmortem examination, the wall of a circumscribed region of the colon was involved with emphysematous lesion characterized by the presence of many small gas bubbles. Histologically, this emphysematous lesion proved to be multicystic lymphangiectasis, mainly involving the submucosal tissue. Lymphangiectasis was associated occasionally with granulomatous infiltrates comprised of giant cells and macrophages. A small number of macrophages and eosinophils infiltrated into areas su...
Source: Comparative Clinical Pathology - May 22, 2017 Category: Pathology Source Type: research

Comparing the Vitex-castus extract and cabergoline effects on estrous induction in female dogs
AbstractCabergoline is used to induce estrus, stop lactation, and induce abortion in small animals. The specific doses of Vitagnus extract have almost identical properties of cabergoline that reduce prolactin and increase the concentration of FSH hormone. The aim of this study was to compare the effect ofVitex agnus-castus extract and cabergoline on prolactin concentration and hormonal changes associated with the onset of estrous cycle in dogs. Twelve healthy adult cyclic female-mix breed dogs were selected and randomly aligned in treatment (6 dogs, Vitagnus extract 1.5  ml daily for 5 weeks, 80–90 mg/...
Source: Comparative Clinical Pathology - May 21, 2017 Category: Pathology Source Type: research

Reference values for some clinical, hematobiochemical and electrocardiographic parameters in miniature donkeys: the effect of sex, age and season
AbstractIn the current study, we aimed to establish reference values for the most important clinical, hematobiochemical and electrocardiographic (ECG) parameters and evaluate the effect of sex, age and season in miniature donkey. We hypothesized that these parameters in this valuable breed of donkey would be different from those of other species of equidae. Twenty apparently healthy miniature donkeys aged 1 –8 years and weighing averagely 115 ± 10 kg, with an average height of 0.83 ± 0.3 m, were used in this study. Based on sex, age and season of sampling, the animals ...
Source: Comparative Clinical Pathology - May 19, 2017 Category: Pathology Source Type: research

Monepantel in the control of Haemonchus spp. and Trichostrongylus spp. and possible side effects of treatment in naturally infected sheep
AbstractSheep breeding represents an important economic source in many countries; however, anthelmintic resistance is an important limiting factor for sheep breeding development, due to its effects in animal performance. Monepantel is an active principle derived from an amino-acetonitrile class, with proven efficacy in the control of gastrointestinal parasites. However, the side effects associated to this treatment remain unknown. Thus, the aim of this study was to evaluate the effect of anthelmintic treatment with monepantel as well as evaluate the hepatic function, proteic and lipid metabolism, and antioxidant/oxidant le...
Source: Comparative Clinical Pathology - May 17, 2017 Category: Pathology Source Type: research

Topical co-administration of Pistacia atlantica hull and Quercus infectoria gall hydroethanolic extract improves wound-healing process
AbstractPistacia atlantica hulls andQuercus infectoria galls have been used in traditional medicine for treatment of many disorders. In the current study, excision wound model was used for the assessment of wound-healing activity of topical co-administration of hydroethanolic extracts ofPistacia atlantica hulls (P. atlantica) andQuercus infectoria galls (Q. infectoria) in streptozotocin-induced diabetic mice. Four groups of diabetic mouse model were used; control group (received soft yellow paraffin), treated groupsP. atlantica 5%;Q. infectoria 5% andQ. infectoria 5%  + P. atlantica 5% mixed soft yellow paraffin....
Source: Comparative Clinical Pathology - May 13, 2017 Category: Pathology Source Type: research

Isolation and identification Candida vulvovaginitis in women referred to health centers of Arak City and antifungal activity of Equisetum arvense and Quercus on Candida albicans
This study investigated 152 patients suspicious of vulvovaginal candidiasis. Seventy-five isolates were identified from 152 isolates ofCandida albicans, and 55 yeast isolates were identified as non-albicans (Candida krusei,Candida glabrata, andCandida tropicalis), and 22 yeast isolates were unidentified. In broth microdilution method,MIC50 was reported as 12.5  μl/ml inCandida albicans forEquisetum arvense andQuercus, and MFC was reported as 25  μl/ml for both plants. In this research, isolation ofCandida albicans species is more than that of non-albicans species. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 13, 2017 Category: Pathology Source Type: research

Impact of iron deficiency anemia on CD4 and CD8-T lymphocytes among preschool-school children
AbstractIron is a basic element at cellular and molecular levels for proper development and effective function of the immune system. Children with iron deficiency are more susceptible to infections and have many alterations to their immune profile. The current study investigates the quantitative changes for CD4 and CD8 T-lymphocytes among symptomatic and asymptomatic iron deficient children. Flow cytometry using conjugated monoclonal antibodies was used for assessment of the CD4 and CD8 T-lymphocyte subsets among 30 asymptomatic iron deficient children, 28 iron deficiency anemia (IDA) symptomatic children, and 30 healthy c...
Source: Comparative Clinical Pathology - May 11, 2017 Category: Pathology Source Type: research

Effects of Salvia officinalis L. on 7, 12-dimethylbenz[a]anthracene-induced breast cancer in rats and mouse mammary carcinoma cells (4T1)
AbstractThe toxicity ofSalvia officinalis L. on different cancer cell lines has been reported extensively, but an in vivo evaluation on breast cancer has not been conducted. Therefore, in vivo and in vitro studies of are evaluated in this study. The hydro-alcoholic extract ofSalvia officinalis L. was studied in female Wistar rats in three groups: one control (Con) group (n = 7) and two 7, 12-dimethylbenz[a]anthracene cancer-induced groups (CI) that were gavaged either with distilled water (Con-CI) or 30 mg/kg sage hydro-alcoholic extract (SHE-CI) for 30 days (n = 34). A mammary gland whole mou...
Source: Comparative Clinical Pathology - May 10, 2017 Category: Pathology Source Type: research

Hematological and serum biochemical alterations in buffalo with some digestive disorders
AbstractHematological and serum biochemical reference intervals are essential laboratory variables for optimal diagnostic purposes. The present study was delineated to provide an insight into the hemato-biochemical alterations as well as the measurement of selected acute phase proteins in buffaloes showing some digestive disorders. The present study included 40 native breed water buffalo showing various symptoms of digestive troubles. Based on competent case history, clinical and laboratory findings, the diseased buffaloes were allocated into four equal sized groups, namely stomatitis, acute traumatic reticuloperitonitis (...
Source: Comparative Clinical Pathology - May 10, 2017 Category: Pathology Source Type: research

Cryptosporidium genotypes and associated risk factors in a cohort of Egyptian children
AbstractCryptosporidium is one of the most common, worldwide diarrheal diseases caused by parasites. Due to absence of an effective treatment, determining the prevailing species ofCryptosporidium is a key in identifying its transmission dynamics and a necessary precursor required for the planning and implementation of effective preventive and control strategies. This PCR-RFLP study was done to determine the prevalence ofCryptosporidium species in the stool of a cohort of Egyptian children and evaluate/assess associated risk factors for susceptibility to cryptosporidiosis, due to the lack of existent studies addressingCrypt...
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

Generalized lower motor neuron paralysis associated with Toxocara canis in a husky puppy
AbstractToxocara canis is a parasitic, ascarid nematode considered to be one of the most prevalent gastrointestinal helminths of domestic dogs and other canids worldwide. The larvae of this parasite in dogs migrate to the lungs and liver to complete the life cycle, thus respiratory symptoms such as cough and nasal discharge and vomiting may be observed. Neurological symptoms in human beings have been reported due to migration of the larvae of the parasite to the central nervous system. Beside, neurotoxocariasis associated with lower motor neuron paralysis has been reported in a male individual but such neurological symptom...
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

Prevalence of Sarcocystis infection in processed meat products by using digestion and impression smear methods in Hamedan, Iran
This study aimed to detectSarcocystis parasite in processed meat products in Hamedan, Iran. A total of 20 samples of hamburger, sausage, and cocktail were collected from markets from three factories in Hamedan, Iran. The samples were examined by digestion and impression smear methods for detectingSarcocystis parasite. The results showed that 80% of all tested samples were infected withSarcocystis. The infection rate in hamburger, sausage, and cocktail were 87.5, 83.33, and 66.66%, respectively. The highest infestation rate was observed in hamburger. The present study shows that the rate ofSarcocystis contamination in meat ...
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

Preliminary report on the age-related variations in the serum and seminal plasma testosterone, seminal plasma biochemistry and seminal vesicle histochemistry of male broodstock African catfish ( Clarias gariepinus )
This study investigated the age-related changes in the concentrations of serum testosterone and seminal plasma testosterone, ions, total protein, glucose concentrations and alkaline phosphatase (AP), amino aspartate transferase (AST) activities and the periodic acid Schiff-Alcian blue histochemistry of the seminal vesicle of cultured male broodstock African catfish of 6, 9 and 12  months of age. Each of these months constituted an age group and had five fish. The mean seminal plasma testosterone concentrations of the 6 months-age group were significantly higher (P 
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

TRPV1 gene polymorphisms in patients with diabetes compared with healthy individuals
In this study, no significant association was found betweenTRPV1 gene polymorphisms and the presence of type 1 diabetes. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 8, 2017 Category: Pathology Source Type: research

The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats
AbstractDiabetic retinopathy is a metabolic disease and oxidative stress-associated diabetic disorder. The antioxidant plants are important in the treatment of diabetic mellitus. The main purpose of this study was to determine the effect ofAmygdalus scoparia on anti-diabetic and antioxidant activity in the photoreceptor layer of diabetic retina. Thirty rats were housed and divided into three groups of 10 rats each. Group I: control group; group II: Diabetic group (induced by IP injection of 50  mg/kg of stereptozotocin); group III: diabetic receiving 200 mg/kgA. scoparia extract 1  month before and 2 mo...
Source: Comparative Clinical Pathology - May 8, 2017 Category: Pathology Source Type: research

Association between oestrogen receptor β immunoexpression and cause of culling, ovarian appearance and the existence of PRRS virus in the porcine ovary
In conclusion, the gilts culled due to anoestrus had relatively poor ovarian ERβ immunoexpression. Neither the existence of PRRS virus nor ovarian appearance was associated with ERβ immunoexpression in the gilt ovary. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 5, 2017 Category: Pathology Source Type: research

Cure rate of postpartum endometritis after different treatments in high produce dairy cows
AbstractThe main objective of this study was to compare the common treatment methods of clinical endometritis (CE) including injection of PGF2a and intrauterine infusion of cephapirin benzathine (CB) with intrauterine infusion of 50% dextrose solution (DEX) and intrauterine infusion of liquid paraffin (LP) in dairy cows. To this end, 188 lactating Holstein cows with clinical signs of endometritis were randomly assigned into five treatment groups and cytological smear was prepared. In group 1 (n = 37), cows were treated with intrauterine infusion of CB. In the second group (n = 53), the treatment protoco...
Source: Comparative Clinical Pathology - May 4, 2017 Category: Pathology Source Type: research

Respiratory tract cadmium-induced injuries —poisoning via intake and water pH could influence their genesis? An experimental study in rats
AbstractCadmium exposure may result in a variety of pulmonary diseases. No studies have evaluated tracheal damage or even whether cadmium intake can damage the respiratory tract. We evaluated the possible injuries caused by cadmium poisoning via intake into the respiratory tract and the possible effects of water pH in their genesis. Ninety male Wistar rats were divided into six groups (n = 15): GC5—received CdCl2 (400 mg/l) in the drinking water at an acidic pH of 5; GC7 received CdCl2 (400 mg/l) in the drinking water at an acidic pH of 7.0; GC8—received CdCl2 (400 mg/l) in the drinking...
Source: Comparative Clinical Pathology - May 3, 2017 Category: Pathology Source Type: research

Primary form of malignant melanoma in an ovarian mature cystic teratoma: case report and literature review
AbstractGerm cell tumours are a common form of ovarian neoplasm; the most common of which is mature cystic teratoma. However, transformation into malignant melanoma is rare. Here, we present a case of a primary malignant melanoma in an ovarian mature cystic teratoma with literature review. A 63-year-old woman presented at our hospital with abdominal distension and pain. Physical examination showed a mobile, non-tender, abdominopelvic mass. Radiological examination revealed a cystic mass with mixed echogenicity. Histopathological review disclosed a primary malignant melanoma arising in an ovarian mature cystic teratoma. The...
Source: Comparative Clinical Pathology - May 2, 2017 Category: Pathology Source Type: research

Clinicopathological impact of CD20 expression in childhood B cell precursor acute lymphoblastic leukemia (BCP-ALL)
This study aimed to evaluate the efficacy of CD20 expression as a biological marker in BCP-ALL. For 104 children with newly diagnosed BCP-ALL included in this study, CD20 expression was evaluated by flow cytometry. CD20 was positively expressed in 40 (38.5%) of cases. The CD20positive subgroup had significantly shorter overall survival and disease free survival as compared to the CD20negative one (P = 0.018;
Source: Comparative Clinical Pathology - May 1, 2017 Category: Pathology Source Type: research

RAPD-PCR analysis of Staphylococcus aureus strains isolated from different sources
AbstractDetermination of genetic relationship amongStaphylococcus aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine the molecular diversity ofS. aureus strains isolated from human, bovine, and food samples using random amplified polymorphic DNA (RAPD)-PCR method. A total of 208S. aureus strains including 60 human (patients and healthy carriers), 83 bovine milk, and 65 food (pastry and cheese) isolates were assessed. The isolates were confirmed to be methicillin-resistance/susceptibleS. aureus (MRSA or MSSA) using co...
Source: Comparative Clinical Pathology - April 27, 2017 Category: Pathology Source Type: research

Significance of cardiac and iron profile alteration in diabetic patients
This study was conducted on 120 subjects. Subjects were divided into two main groups (80 diabetic patients and 40 healthy controls); diabetic subjects were subdivided into two subgroups (G-IIa and G-IIb) according to fasting blood glucose and glycosylated hemoglobin (HbA1c). Serum iron profile (iron, total iron binding capacity, and percentage transferrin saturation) is calculated as the index of iron. Lactate dehydrogenase (LDH), total creatine kinase (CK), and creatine kinase-MB (CK-MB) activities are measured as an index of cardiac functions. Also, fasting blood glucose and HbA1c were assayed. Diabetic patients were cha...
Source: Comparative Clinical Pathology - April 26, 2017 Category: Pathology Source Type: research

Phyllodes tumors are an uncommon differential diagnosis of axillary mass
AbstractPhyllodes tumors are rare fibroepithelial neoplasm that account for less than 0.5% of all breast tumors and presentation in ectopic breast tissue is even rarer. In this paper, we describe a case of a phyllodes tumor in the ectopic breast tissue of the axilla. A 40-year-old woman presented with a well-circumscribed, firm, and freely mobile mass in the left axilla from 1  year ago. Ultrasound revealed a 5.5 × 4.5 × 4.5 cm homogenous and hypoecho lesion. The histopathologic analysis revealed that it was a benign phyllodes tumor. Phyllodes tumors in ectopic breast tissue may be...
Source: Comparative Clinical Pathology - April 25, 2017 Category: Pathology Source Type: research

Kinetics of inhibitory action of Myrothamnus flabellifolius Welw. on the activities of α-amylase and α-glucosidase
AbstractRecently, efforts have been geared towards the development of alternative medicines to manage diabetes mellitus by searching for pharmacological agents with the ability to retard glucose absorption through inhibition of α-amylase and α-glucosidase. The present study investigated the in vitro antioxidant capability and inhibitory potential ofMyrothamnus flabellifolius leaf extracts on the activities of α-amylase and α-glucosidase. The hydroethanol extract elicited significant α-glucosidase inhibitory effect (IC50 = 0.50 ± 0.04 mg/mL) and a mild α-am...
Source: Comparative Clinical Pathology - April 20, 2017 Category: Pathology Source Type: research

Nicorandil reverses the behavioral changes and brain oxidative stress in a model of schizophrenia induced by ketamine in mice
We examined the effect of the KATP channel opener nicorandil and the KATP channel blocker glibenclamide on the behavioral and biochemical responses to ketamine in mice. Ketamine was injected at 30  mg/kg intraperitoneally (i.p.) daily for 2 weeks, and mice were then treated with either saline, glibenclamide (5 mg/kg), nicorandil (1 or 5 mg/kg), sucrose 1 g/kg, or glibenclamide + sucrose 1 g/kg, i.p. daily for another 8 days. The control group received only saline. Data indicated that ( i) ketamine increased locomotion, rearing, and grooming behaviors. Locomotor activity increased in the c...
Source: Comparative Clinical Pathology - April 20, 2017 Category: Pathology Source Type: research

Potential pathogens in infertile male dromedary camels and their association with the spermiogram and clinical findings
AbstractThe role of five pathogens potentially implicated in the reproductive failure of 141 male dromedary camels was investigated. Breeding soundness examinations, which included semen analysis and testicular histopathology of the infertile male camels, were compared with seven camels of normal fertility. Antibodies againstChlamydia abortus (C. abortus) andToxoplasma gondii (T. gondii) were detected using the ELISA assay. Antibodies againstTrypanosoma evansi (T. evansi) were identified using the card agglutination test. The Rose Bengal plate agglutination test was used to detectBrucella species. Preputial swabs were cult...
Source: Comparative Clinical Pathology - April 18, 2017 Category: Pathology Source Type: research

Relation between diarrhea and infection by protozoans in dairy calves
In this study, newborn calves were selected from farms with history of protozoa infections and diarrhea. A total of 26 calves were used in the study, 18 were positives forGiardia spp., 17 forEmeria spp., and 21 forCryptosporidium spp., considering single and mixed infections. Furthermore, the survival rate of calves due to the presence of diarrhea was significantly different compared to the group without diarrhea, for all three protozoa. In addition, according to this study, it is possible to indicate that the occurrence of diarrhea is highly correlated to the presence ofGiardia sp., leading to economic losses due to a low...
Source: Comparative Clinical Pathology - April 18, 2017 Category: Pathology Source Type: research

Some haematological parameters of albino mice experimentally inoculated with Trypanosoma brucei brucei after administration of Halofantrine hydrochloride, chloroquine and diaminazine aceturate
AbstractTrypanosomosis has been a menace. When animals become infected with trypanosomosis, their physiology is altered leading to haematological aberrations. The comparative effects of Halofantrine chloride (Halfan), chloroquine and diaminazine aceturate (DA) were experimentally investigated in the treatment ofTrypanosoma brucei brucei infected albino mice. This is to know whether Halfan and chloroquine can provide alternatives to diaminazine aceturate. White albino mice experimentally infected withT. brucei brucei which were separately treated 7  days post infection with Halfan at 100 mg/kg body weight as a sin...
Source: Comparative Clinical Pathology - April 14, 2017 Category: Pathology Source Type: research

The evolving hemostatic profile of patients with myeloma receiving treatment
AbstractThrombosis is a well-described complication of myeloma and the drugs used to treat it. The causes are multifactorial. Traditional screening tests of coagulation, such as the prothrombin time and the activated partial thromboplastin time, are known to be poor at assessment of thrombosis risk. It has been suggested that global measures of haemostasis, such as thromboelastography, may have a role to play in the assessment of this risk. The aim of this study was to look at traditional measures of haemostasis, that measure certain parts of the coagulation cascade, as well as thromboelastography, to see if a coagulation ...
Source: Comparative Clinical Pathology - April 11, 2017 Category: Pathology Source Type: research

Effects of short-term repeated fasting on anesthesia: an experimental animal study
The objective of this study was to examine the effect of short-term repeated fasting on some anesthetic parameters of ketamine and xylazine, two anesthetics routinely used in rodents. We divided 50 male mice equally into a fasting and a control group. The fasting group was fasted for 4 continuous days every 2  weeks, and this cycle was repeated four times. During the fasting periods, the fasting mice had access to water only, whereas the control group had free access to both water and food. The mice were allowed a week to recover, then each mouse was anesthetized with a ketamine and xylazine (100 mg/kg and 5 ...
Source: Comparative Clinical Pathology - April 10, 2017 Category: Pathology Source Type: research

The prevalence of pigeon fly ( Pseudolynchia canariensis ) and its phoretic association with mites in domestic pigeons kept in west of Iran
The objectives of this study were to determine the prevalence of pigeon fly, identify its possible phoretic association with mites, and determine the prevalence of hemoproteid parasites in domestic pigeons (Columba livia domestica) in Kermanshah province, west of Iran. From May 2015 to May 2016, 16 colonies of domestic pigeons were investigated. In case of infestation, the flies were fixed in ethanol containing glycerin and then placed in potassium hydroxide. The flies were identified based on morphological characteristics. The masses suspected to contain mites were removed from the body surfaces of flies by centrifugation...
Source: Comparative Clinical Pathology - April 10, 2017 Category: Pathology Source Type: research

An association study between CHEK2 gene mutations and susceptibility to breast cancer
AbstractCHEK2 gene is known as a tumor suppressor gene in breast cancer (BC), which plays a role in DNA repair. The germ line mutations in CEHK2 have been associated with different types of cancer. The present study was aimed at studying the association between CHEK2 mutations and BC. Peripheral blood was collected from patients into a test tube containing EDTA, and DNA was extracted from blood samples. Then, we analyzed mutations including 1100delc, IVS2+1>A, del5395bp, and I157T within CHEK2 gene in patients with BC and 100 normal healthy controls according to PCR-RFLP, allelic specific PCR, and multiplex-PCR. Althoug...
Source: Comparative Clinical Pathology - April 8, 2017 Category: Pathology Source Type: research

Effect of short-term administration of methionine on the ovary and uterus in a rat
AbstractMethionine is necessary for development but overuse of it can not only cause growth retardation but also damage organs such as the liver and cardiovascular system. The purpose of this research was to determine the effect of short-term administration of methionine on the ovaries and uterus in a rat. Forty adult female rats were randomly allocated into four equal groups: each received 50, 100 or 200  mg/kg BWl-methionine in 0.5  mL normal saline or just 0.5 mL normal saline (control) intraperitoneally for 20 days. On day 21, all rats were euthanized and tissues were taken for histological evaluati...
Source: Comparative Clinical Pathology - March 30, 2017 Category: Pathology Source Type: research

Immunohematological status under acute ammonia stress of juvenile rainbow trout ( Oncorhynchus mykiss Walbaum, 1792) fed garlic ( Allium sativum ) powder-supplemented meat and bone meal-based feeds
AbstractA feeding trial was conducted for 60  days to determine the effects of fish meal (FM) replacement with meat and bone meal (MBM) supplemented with garlic powder on the physiological and immunohematological status of juvenile rainbow trout (Oncorhynchus mykiss; 8.26  ± 1.10 g). Eight isonitrogenous (46% crude protein) and isoenergetic (22 mJ kg−1) diets were formulated containing increasing levels of MBM 0 (control), 25, 45, and 65% with or without garlic powder (3%). Our findings indicated that garlic supplementation increased red blood cell (RBC), hemoglobin (Hb), hematocr...
Source: Comparative Clinical Pathology - March 25, 2017 Category: Pathology Source Type: research

Helicobacter pylori cytotoxin-associated gene A genotype in Egyptian patients with Parkinson ’s disease: could eradication benefit?
This study was performed on 87 idiopathic PD patients and 45 age- and sex-matched controls. HP infection was diagnosed with HP stool antigen test and HPcagA genotype was detected by PCR. PD patients were subjected to UCLA parkinsonism scale, Hoehn and Yahr staging, andl-dopa plasma level detection by high-performance liquid chromatography. HP PD patients were divided into eradicated group (received standard anti-HP therapy) and placebo group (received antioxidant therapy). Four weeks after therapy, patients were reevaluated. Among PD patients, frequency of HP infection was significantly higher than controls (55.2 vs 33.3%,...
Source: Comparative Clinical Pathology - March 23, 2017 Category: Pathology Source Type: research

Lead (Pb), a threat to protein metabolic efficacy of liver, kidney and muscle in mice
AbstractLead, being the most dangerous natural heavy metal imposes metabolic dysfunction in organ system. However, the effect of lead on protein metabolic efficacy in the liver, kidney and skeletal muscle in mice has not been well studied. The aim of the present study is to establish the metabolic orientation between the liver, kidney and muscle upon sub-acute lead exposure to assess how protein and amino acid metabolisms are affected. Lead acetate was administered by gavage at a dose of 5  mg/kg b.w./once daily for 30 days. After the treatment, the liver, kidney and muscle were evaluated for biochemical par...
Source: Comparative Clinical Pathology - March 22, 2017 Category: Pathology Source Type: research

Microbiologic and histopathological findings of osteomyelitis in diabetic patients
AbstractOsteomyelitis can present as a severe complication in diabetic patients. The present study was structured to evaluate the histopathological characteristics of bone infection in the feet of diabetic patients and to associate any clinical variables seen with each type of bone infection. Eighty-four cases of osteomyelitis were evaluated microbiologically and histopathologically after samples were collected under sterile conditions in an operating room. The data generated were analysed using SPSS software. Polymorphonuclear neutrophil (PMN) infiltrates were associated with acute osteomyelitis (AO) (22 cases (26.2%)) an...
Source: Comparative Clinical Pathology - March 17, 2017 Category: Pathology Source Type: research

Estimation of hematological reference intervals for clinically healthy Iraqi local goats using reference value advisor
AbstractA cross section study was conducted to investigate the effect of sex and to establish reference values for hematological parameters in the Iraqi local goats in order to form a basis for clinical interpretation. A total of 120 goats (59 male and 61 female) were enrolled in the current study during the period from November to December 2016. The animal ’s age ranged from 1 to 5 years. Statistical analysis showed that Hct, MCV, and WBC means were significantly (P 
Source: Comparative Clinical Pathology - March 16, 2017 Category: Pathology Source Type: research

Effect of phytobiotic (turmeric) supplementation on semen and blood characteristics of rabbits
AbstractThe study aimed to examine the effect of turmeric (Curcuma longa) powder supplemented diets on semen and blood characteristics of rabbits. A total of 27, 9-month-old New Zealand white male rabbits were assigned randomly into 3 groups with 3 replicates of 3 rabbits. Each group was assigned to one of the experimental diet containing turmeric powder at 0 (control), 2 and 4  g/kg feed for 84 days in a completely randomized design. Blood and semen samples for analysis were obtained from each replicate and data obtained analysed statistically. Results revealed that there are significant (p 
Source: Comparative Clinical Pathology - March 16, 2017 Category: Pathology Source Type: research

Assessment of 25-hydroxy vitamin D in stroke patients based on severity and type: a cross-sectional study
This study showed that low 25(OH) vit D serum level is common in male but there was not any relation between serum level of 25(OH) vit D, type, and severity of stroke. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 16, 2017 Category: Pathology Source Type: research

Comparative biochemical, parasitological, and histopathological studies on cystic echinococcosis in infected sheep
This study was done to investigate the parasitological studies and biochemical and histopathological changes of hydatid cyst infection in livers of sheep. Our study was carried out prospectively on sheep slaughtered in a southern slaughterhouse, Hail city, conducted from January 2015 to December 2015 to determine the prevalence, cyst viability, and biochemical analysis of fertile and infertile hydatid fluids. Out of 53,981 slaughtered sheep, 3811 were found infected with hydatid cyst; specifically, hydatid cyst was detected in the liver of sheep. The overall infection rate in slaughtered sheep in this period (from 1 Januar...
Source: Comparative Clinical Pathology - March 13, 2017 Category: Pathology Source Type: research

Erythrogram, leukogram, and acute phase protein reference intervals for healthy newborn Murrah buffalo calves ( Bubalus bubalis ) within the first month of life
In conclusion, hematologic and acute phase protein RI have been documented and can be used as a physiologic database to help the i nterpretation of laboratory results of newborn buffaloes during infection and inflammation conditions. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 10, 2017 Category: Pathology Source Type: research

Does ketamine-midazolam combination act as a routine and safe chemical restraint in cats? Clinical and hemato-biochemical evaluation
AbstractIntramuscular administration of ketamine-midazolam was used as a fast-acting chemical restraint, with convenient use of the drug in domestic cats. Furthermore, biochemical and hematological changes were evaluated following administration. A total of 20 clinically healthy cats were selected. During the physical examination, cephalic vein was prepared aseptically. Then, cats were restrained cautiously and intravenous catheter was placed in the cephalic vein and immediately heparinized. After covering and protecting the catheter, the animals were kept in separate cages for half an hour. Subsequently, the first blood s...
Source: Comparative Clinical Pathology - March 8, 2017 Category: Pathology Source Type: research

Expression of β-defensins in bovine oviduct and uterus during follicular and luteal phases
This study was conducted to examine the expression of β-defensins of cow’s reproductive tract and their relationship with estrus cycle using quantitative PCR and ELISA methods. Six kinds of β-defensins and the receptors of estrogen and progesterone were studied in cows’ reproductive tract during the follicular and luteal phases. The results show that besides LAP, TAP, BNBD4, and BNBD5, BNBD1 and BNBD10 were also found in cow oviduct, uterine horn, and uterine body. During the follicular phase, the uterine body had the highest expressions of BNBDI, TAP, LAP1, BNBD4, and BNBD5. However, the highest expr...
Source: Comparative Clinical Pathology - March 8, 2017 Category: Pathology Source Type: research

Ca 2+ sensitivity of skinned ventricular cardiac muscle and expression of cardiac myosin heavy chain isoforms in hibernating versus active frogs
This study was designed to find the changes in myosin heavy chain (MHC) isoforms and their relationship to calcium sensitivity in frog ventricular muscle during hibernation. MHC profile was determined by SDS-4% polyacrylamide gel electrophoresis in active and hibernating frogs for different intervals of time (10, 20, 30, and 60  days). Calcium sensitivity of Triton-skinned ventricular muscle fiber bundles was determined. Compared with active and hibernated frogs for 10 and 20 days, hibernated frogs for 30 and 60 days expressed significant amount (p 
Source: Comparative Clinical Pathology - March 8, 2017 Category: Pathology Source Type: research