Silymarin and methionine application on treatment of liver chronic diseases by aflatoxicosis in rabbit ( Oryctolagus cuniculi ) —case report
AbstractThis case report describes the treatment of chronic liver disease in rabbit (Oryctolagus cuniculi) caused by consumption of feed containing mycotoxin and later treated with silymarin. A young female rabbit was referred to veterinary care with a history of apathy, appetite loss, and difficulty breathing without a clear medical history. The physical examination revealed an increase in the abdominal volume, an observation confirmed by radiographic analysis showing the presence of ascites. Through the serum biochemistry evaluation, it was an evidenced liver disease. After 1  month of treatment, the animal did not ...
Source: Comparative Clinical Pathology - January 13, 2017 Category: Pathology Source Type: research

Farm-level evaluation of enrofloxacin resistance in Escherichia coli isolated from broiler chickens during a rearing period
In this study, enrofloxacin resistance was investigated in commensalEscherichia coli isolated from broiler chickens using phenotypic and genotypic assays. FiveE. coli isolates were recovered from the cloacal swabs of 15 broiler chickens in the farms at the ages of 1  day (stage 1), 30 days (stage 2), and 1 day before slaughter (stage 3). Minimum inhibitory concentration results for enrofloxacin showed that the prevalence of resistance significantly increased (P 
Source: Comparative Clinical Pathology - January 12, 2017 Category: Pathology Source Type: research

Stability of some clinical biochemistry parameters in equine serum/plasma stored at refrigerator and room temperatures: a preliminary study
AbstractAccurate measurement of clinical biochemistry parameters is an essential tool for the correct interpretation of clinical biochemistry abnormalities. A general problem faced by most clinical laboratories is inability to process blood samples received late or submitted in large numbers. This delay in sample processing is usually unavoidable. This present study evaluated the stability of some biochemistry parameters in equine blood before and after storage at room (25  °C) and refrigerator (4 °C) temperatures. Five milliliters of blood were collected from external jugular veins of 17 apparently healt...
Source: Comparative Clinical Pathology - January 10, 2017 Category: Pathology Source Type: research

Influence of dexamethasone-induced stress on oxidative stress biomarkers in non-pregnant does experimentally infected with Brucella melitensis
This study aimed at evaluating biomarkers of oxidative stress in dexamethasone-treated non-pregnant Boer goats experimentally infected withB. melitensis. Eighteen healthy, non-pregnant goats were divided into three groups: A, B, and C of six animals each. Group A was treated with dexamethasone (2  mg/kg) for 7 days prior to inoculation with 107 CFU ofB. melitensis ocularly. Group B was not treated but inoculated with similar dose ofB. melitensis as in group A above while group C was administered normal saline. Blood samples were collected periodically through day 42  post inoculation (pi). Plasma cortis...
Source: Comparative Clinical Pathology - January 7, 2017 Category: Pathology Source Type: research

Evaluation and comparison of a set of oxidative and antioxidative biomarkers in cystinuric patients with age- and sex-matched healthy subjects
AbstractThe present study evaluates and compares the serum biomarkers of oxidative stress —superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), malondialdehyde (MDA), glutathione reductase (GR), and total antioxidant capacity (T-AOC), in a case-control design study of 30 diagnosed patients with cystine stone and 30 sex- and age-matched healthy control group. The levels of SOD were significantly lower in patients (14.47 ± 15.13 ng/mL) when compared with control group (26.18 ± 15.34 ng/mL),p value 0.003. Similarly, the levels of iNOS were significantly lower in p...
Source: Comparative Clinical Pathology - January 6, 2017 Category: Pathology Source Type: research

Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction)
AbstractEthanolic extract of bitter fraction ofStevia rebaudiana (Srbf) was extracted to investigate its antihyperglycemic and protective effects on renal structural changes in STZ-induced diabetes. Thirty-five male mice were divided into five groups randomly; the first group as non-diabetic control, the second group as untreated diabetic, the third group treated with glibenclamide 0.5  mg/kg, and the fourth and fifth groups treated withSrbf by 200 and 400  μg/kg bw through gavage, respectively, for 15 days. Diabetes was induced in the second to fifth groups by administration of 60 mg/kg bw of strept...
Source: Comparative Clinical Pathology - January 3, 2017 Category: Pathology Source Type: research

Histopathological assessment of chronic Corynebacterium pseudotuberculosis infection in the reproductive tract and iliac lymph node of Katjang does
In this study, 18 non-pregnant healthy Katjang does aged 2  years old were randomly divided into two groups of nine goats each. The first group were all experimentally inoculated with 1 ml of 107 cfu of liveC. pseudotuberculosis through intradermal route while the second group was given 1  ml PBS (pH 7) intradermally. Three animals from each group were withdrawn and culled after 30, 60 and 90 days post infection and the reproductive organs (vagina, cervix, uterus, uterine horns, and ovaries) and iliac lymph nodes were collected in 10 % buffered formalin, processed, sectioned, and stained ...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Prevalence and intensity of parasitic infection in domestic ducks ( Anas platyrhynchas ) in Gilan Province, Northern Iran
This study was carried out to assess the levels of parasitic infection in domestic ducks (Anas platyrhynchas) in Gilan Province, Northern Iran. During the summer of 2015, samples were collected randomly from local markets at different villages in Gilan Province. Blood samples were collected in EDTA-treated tubes, blood smear slides created, stained with giemsa and examined for the presence of blood protozoa. Ectoparasites samples were collected and identified according to Wall and Shearer (2001). To assess endoparasitic infection, the ducks ’ gastrointestinal tracts were removed and the various parts identified and o...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Occurrence of gastrointestinal helminths in horses and risk factors for infection
This study aimed to monitor the occurrence of gastrointestinal parasites in horses evaluating different rural properties in Santa Catarina State, Southern Brazil. Fresh samples of feces were collected and processed by the McMaster technique, quantifying the number of eggs per gram of feces (EPG), followed by coproculture for identification of helminths. The population investigated was composed of 111 horses of varying ages (1 –27 years, 63 males and 48 females). Among all the animals evaluated, 76 animals showed EPG ≥50, representing 63.96 % (IC95  % 54.24 –72.70) of samples positive for gastr...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Butyrylcholinesterase activity in dairy cows naturally infected by Dictyocaulus viviparous and treated with eprinomectin
AbstractThe aim of this study was to evaluate the activity of butyrylcholinesterase (BChE) in dairy cows naturally infected byDictyocaulus viviparus and treated with eprinomectin. Among 22 serum samples, 10 were of animals with mild clinical disease and 12 with severe clinical disease. The samples were collect on days 0 (day of diagnosis) and 10 post-treatment (a single dose of eprinomectin [360  μg/kg, topically]). We evaluated the activity of BChE, alanine aminotransferase (ALT), aminotransferase andaspartate (AST), and gamma-glutamyl transferase (GGT) in serum. There was no significant difference between groups ...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Visceral and serum lysozyme activities in some freshwater fish (three catfish and two carps)
AbstractThe lysozyme activities were investigated in three catfish: hybrid catfish [Clarias macrocephalus (G ünther 1864) xClarias gariepinus (Burchell 1822)],Hemibagrus filamentus (Fang and Chaux 1949), andKryptopterus apogon (Bleeker 1851) and two carps:Oreochromis niloticus (Linnaeus 1758) andChanna striata (Bloch 1793). Fish samples used in this study were from two sites of Chiang Mai province;C. macrocephalus xC. gariepinus andO. niloticus were from Ton Payom Market, Amphoe Muang.H. filamentus,K. apogon, andC. striata were from Doi Tao Lake, Amphoe Doi Tao. The serum, brain, liver, and kidney of the catfish and c...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Effects of glutamine and glutamate supplementation in dogs with hemorrhagic enteritis
This study evaluated the effect of glutamine and glutamate supplementation on hematological and biochemical biomarkers in dogs with clinical enteritis. Fifteen young dogs (3 –10 months) with clinical enteritis were divided into two groups: group 1 (G-CON)—five animals subjected to medical treatment without supplementation and group 2 (G-GLN)—ten animals subjected to medical treatment and orally supplemented with 0.5 g/Kg per day of a glutamine and glutamate for 14 days. The following variables were measured: blood count, plasma glutamine and glutamate, total plasma protein (TPP), albumin, g...
Source: Comparative Clinical Pathology - December 31, 2016 Category: Pathology Source Type: research

Flow cytometry analysis of lymphocyte subsets in bronchoalveolar lavage: comparison between lung non-Hodgkin lymphomas and reactive diseases
AbstractDeepened flow cytometry (FCM) analysis of bronchoalveolar lavage fluid (BALF) cells can disclose clonal B and abnormal T lymphocytes in case of lung involvement in non Hodgkin lymphoma (NHL). The possible role of routine FCM BALF analysis in diagnosing NHL involving the lungs is largely undetermined. To evaluate whether differences exist, within FCM screening of lymphocyte subsets, between BALFs from lung NHL and BALFs from reactive diseases. We compared alveolar leukocyte and lymphocyte data obtained using flow cytometry in 17 lung NHL cases with the median corresponding data detected in 208 controls, matched with...
Source: Comparative Clinical Pathology - December 28, 2016 Category: Pathology Source Type: research

Differentiation of populations with different fluorescence intensities with a machine-learning based classifier
AbstractAs proven fast and reproducible, cellular diagnostics based on machine learning has gained importance lately by its capability to predict different classes of cells based on cell and nuclear morphology, replacing specific markers. The present study aimed to verify if it is possible to differentiate objects with different fluorescence intensities using a machine learning based classifier. Samples were prepared with one drop of fluorescent mounting medium and bead solution (cytocal). Images were generated in .tif format using iCys image cytometer and saved for all relevant channels of fluorescence. Two softwares were...
Source: Comparative Clinical Pathology - December 27, 2016 Category: Pathology Source Type: research

Effects of aqueous and dimethyl sulfoxide extracts of Peganum harmala on juveniles of Dictyocaulus filaria
AbstractThe aim of the present study was to investigate the larvicidal effect of aqueous and dimethyl sulfoxide extracts ofPeganum harmala on larvae ofDictyocaulus filaria. Seed extract was prepared, and serial dilutions of 200, 100, 50, 25, 12.5, 6.25, and 3.12  mg/ml were prepared using distilled water and dimethyl sulfoxide (DMSO). Two genders ofD.filaria were collected from sheep lung and cultured withEscherichia coli bacteria as parasites nutrition source. Ten juveniles were placed in deep slide to assess the larvicidal effects based on disability in the movement, degranulation, and appearance of the wrinkles on ...
Source: Comparative Clinical Pathology - December 26, 2016 Category: Pathology Source Type: research

Assessment of phage cocktails with extended host range activity against antibiotic resistant strains of Pseudomonas aeruginosa
AbstractThe appearance of antibiotic resistant strains ofPseudomonas aeruginosa especially pan-drug resistant (PDR) strains has caused efforts to identify alternative antibacterial methods such as phage therapy. The purposes of this study were the isolation and preparing of effective phage cocktails against antibiotic resistant strains ofP.aeruginosa. Determination of acquired resistance profiles of these isolates was performed according to new definition of CDC and European Centre for Disease Prevention and Control as multidrug-resistant (MDR), extended-drug resistant (XDR) and PDR, and data were analyzed using WHONET5.6 ...
Source: Comparative Clinical Pathology - December 21, 2016 Category: Pathology Source Type: research

Environmental and host factors affecting seroprevalence of bluetongue virus infections of sheep
In this study, the result of testing of 928 samples from a serologic survey of sheep with BTV infections in the Chaharmahal-Va-Bakhtiari province of Iran were analyzed for association between environmental (climate) and host (age, sex, abortion history) factors. Bivariate analysis indicated that environmental and two host factors were associated with the test result. After the chi-square test, a higher seropositive was observed in the plain region (59.94%), compared with the mountain region (24.24%) (df  = 2, χ2 = 104.59,P 
Source: Comparative Clinical Pathology - December 20, 2016 Category: Pathology Source Type: research

Simultaneous application of human amniotic membrane and Tachosil ® in the repair of recto-vaginal fistula in an animal model
AbstractPatients with recto-vaginal fistula (RVF) suffer from distressing symptoms like the passage of stool and gas through the vagina. Although surgeons employed various techniques to address this disorder, however, current techniques are invasive and their success rate is variable. Considering the notable features of human amniotic membrane (HAM) as a bio-prosthesis and Tachosil ® as a type of biologic mesh, the present study was designed to assess the effect of HAM in the presence of Tachosil® on the repair of RVF in a rabbit model. Twelve New Zealand rabbits weighing 2–2.5 kg with the age of 6&ndash...
Source: Comparative Clinical Pathology - December 20, 2016 Category: Pathology Source Type: research

Antioxidant and antileukemic activity of chemical components from bark of Mangifera casturi
In this study, antioxidant and antileukemic activity of chemical components from bark ofMangifera casturi was investigated. Research findings have shown that several extracts ofM. casturi have potent bioactivity. The methanol extract ofM. casturi bark was partitioned successively to yieldn-hexane fraction (6.7%), ethyl acetate (EtOAc) fraction (24.1%), andn-butanol (n-BuOH) fraction (28.1%). Five compounds were isolated from EtOAc fraction and one compound was isolated fromn-hexane fraction. These compounds were identified as methyl gallate (1), taxifolin (2), pyrocatechuic acid (3), gallic acid (4), glucogallin (5), and &...
Source: Comparative Clinical Pathology - December 20, 2016 Category: Pathology Source Type: research

Enrofloxacin stimulates cell death in several tissues of vannamei shrimp ( Litopenaeus vannamei )
AbstractThe aim of this research was to investigate the effect of enrofloxacin on vannamei shrimp (Litopenaeus vanammei) by measuring its accumulation and withdrawal time, in addition to its histopathological effects. The effect of enrofloxacin on the cells of vannamei shrimp has not yet been reported. The enrofloxacin levels were analysed by enzyme-linked immunosorbent assay (ELISA), and several tissues and organs, including the hepatopancreas, gills and muscles, were histopathologically analysed. Regarding the withdrawal time from shrimp tissue, the optimum enrofloxacin dose is 26.06  ± 13.67 μg/...
Source: Comparative Clinical Pathology - December 19, 2016 Category: Pathology Source Type: research

Detection of Clostridium perfringens bacterium in the clinical specimens from blackleg calves: a report of two cases
AbstractThis paper describes the first report involving two cases of blackleg caused byClostridium perfringens Type D as a main causative agent with an emphasis on clinical symptoms, the bacteriological and pathological analyses, and molecular detection of the pathogens by PCR method. Two calves of a flock were presented to the veterinary hospital of Shahid Bahonar University of Kerman, Iran. Both calves died suddenly and showed considerable muscle crepitation in the posterior limb. Severe necrosis of thigh muscles in both hind legs, which had caused dark discoloration of tissue with metallic sheen, large amount of thin sa...
Source: Comparative Clinical Pathology - December 16, 2016 Category: Pathology Source Type: research

Safety assessment of aqueous acetone extract of Clerodendrum infortunatum L. in Wistar rats
AbstractClerodendrum infortunatum L. is a traditional medicinal plant used for the treatment of bronchitis, malarial fever, benign tumour etc. Antioxidant screening revealed that the aqueous acetone extract exhibited the greatest antioxidant activity. In the present study, toxicity analysis of the aqueous acetone extract was carried out in male Wistar rats to evaluate its impact on the liver and serum biochemical parameters. Male Wistar rats were divided into six groups and groups II to VI were treated with a single dose of 250, 500, 1000 and 2000  mg/kg body weight of aqueous acetone extract and silymarin, respective...
Source: Comparative Clinical Pathology - December 16, 2016 Category: Pathology Source Type: research

The diagnostic accuracy of oxidative stress biomarkers in sheep with pneumonic pasteurellosis
AbstractThe goal of this study was to decide the diagnostic accuracy of oxidative stress biomarkers in infected sheep withPasteurella multocida. Blood samples were collected from 56 sheep (36 naturally infected withPasteurella multocida and 20 healthy controls) belonging to one farm in Al-Ahsa region, Saudi Arabia. Serum samples were evaluated for oxidative stress markers (malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT)). Additionally, nasal swabs and bronchoalveolar lavages were gathered from all sheep for bacteriological investigations. Receiver operating characteristic curve was u...
Source: Comparative Clinical Pathology - December 15, 2016 Category: Pathology Source Type: research

Study of illness anorexia in birds by concentrating on roles of central and peripheral cyclooxygenases-1 and cyclooxygenases-2
AbstractProstaglandin E2 (PGE2) is one of the major mediators involved in illness anorexia in rodents. Cyclooxygenases (COX), the important enzymes in prostaglandin synthesizing pathway, have two main isoforms named COX-1 and COX-2. Lipopolysaccharides (LPS) are components of the cell wall of gram-negative bacteria which are used as a standard model of infection. Here, we evaluated the interaction between central and peripheral COX-1 and COX-2 with LPS in neonatal broilers. In the central experiments, the following drugs were injected intracerebroventricularly (ICV): LPS (100  ng), piroxicam, a selective inhibitor of ...
Source: Comparative Clinical Pathology - December 13, 2016 Category: Pathology Source Type: research

Hemostatic and hematological profiles of horses with natural occurrence large colon impaction and medically treated
This study aimed to evaluate the hemostatic and hematological profiles of horses with large colon impaction of natural occurrence and medically treated. For this, ten horses with large colon impaction formed the sick group (SG), and these animals were evaluated at the presentation (T0) and every 24  h for 3 days (T24, T48, and T72). The control group (CG) was composed of ten healthy horses evaluated at the same moments of SG horses (T0–T72). Erythrocyte, leukocyte and platelet parameters, total plasma protein (TPP), fibrinogen, prothrombin time (PT), and activated partial thromboplastin ti me (APTT) were an...
Source: Comparative Clinical Pathology - December 6, 2016 Category: Pathology Source Type: research

Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets
AbstractThe present study was undertaken to examine the physiological effects of early weaning of crossbred piglets and identify a panel of plasma-based biomarkers. Eighteen crossbred (Landrace × indigenous) suckling piglets were assigned to one of the two treatments on the day of weaning: (1) control (n = 9), piglets were loose-housed with their dam and (2) weaned (n = 9), piglets were abruptly selected from their dam and kept in a separate pen. Ad libitum water was supplied to all pens for drinking and all the piglets received same concentrate diet with free access. The blood was collected from ...
Source: Comparative Clinical Pathology - December 2, 2016 Category: Pathology Source Type: research

The effects of preovulatory administration of oral letrozole on ovulatory follicle and the subsequent corpus luteum in mares
AbstractProgesterone acts as an important factor in maintenance of pregnancy and development of equine embryo. Treatment with letrozole, a nonsteroidal inhibitor of aromatase enzyme regulating the synthesis of estrogens, during the preovulatory period has been reported to result in larger corpus lutea (CLs) with greater progesterone production in human and bovine. The present study was conducted to evaluate the effect of preovulatory administration of letrozole on the size of CL and progesterone concentration. In the present study, mares (n = 7) received PGF2α (250 μg) 14 days after the observe...
Source: Comparative Clinical Pathology - November 29, 2016 Category: Pathology Source Type: research

The effect of subclinical ketosis on indices of insulin sensitivity and selected metabolic variables in transition dairy cattle
AbstractUp to now, there have been several endeavors describing the extent of insulin resistance (IR) in dairy cows with particular emphasis on transition period, but it is not fully explicit what IR plays in the etiology of subclinical ketosis (SCK) in transition cows. The present study was therefore conducted on a commercial dairy herd with a stock population of 730 Holstein-Friesian cows to evaluate the extent of IR in transition cows with SCK. Blood samples were collected initially from each of the transition cows (n = 24) to estimate serum levels of β-hydroxybutyrate (BHBA). Cows with no clinical signs ...
Source: Comparative Clinical Pathology - November 25, 2016 Category: Pathology Source Type: research

Injectable mineral supplementation to transition period dairy cows and its effects on animal health
AbstractThe dairy cattle production rises worldwide, with genetically selected animals. Consequently, they present high nutritional requirements, especially during the transition period. The aim of this study was to evaluate the effects of parenteral supplementation of a mineral blend composed by selenium, copper, magnesium, phosphorus, and potassium on hematological, biochemical, immunological, and antioxidant status in transition period dairy cows and newborn calves. The Holstein cows were divided into the control group (A,n = 3) and treated group (B,n = 4), which were supplemented twice with an intra...
Source: Comparative Clinical Pathology - November 24, 2016 Category: Pathology Source Type: research

Haematology and some serum biochemistry of apparently healthy Muturu and Bunaji breeds of cattle in Benue State, Nigeria
This study reports for the first time the haematological and some biochemical parameters for Muturu cattle in Benue State. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 24, 2016 Category: Pathology Source Type: research

Letter to editor: Incorrect identification of study species
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 24, 2016 Category: Pathology Source Type: research

Pathology of muscular steatosis in the bovine species: report of two spontaneously arising cases and comparative overview of the condition
AbstractMuscular steatosis, a condition characterized by prominent fatty replacement of skeletal muscles, has been known from a long time ago to mainly involve meat animals, such as pig, sheep, and cattle. Although a variety of hypotheses are proposed for explaining the condition, its etiology and pathogenesis are still poorly understood. Spontaneously occurring lesions of muscular steatosis were recognized in two 2-year-old steers that were slaughtered for food. Significant clinical abnormalities indicative of motor dysfunction were not observed in these animals. Postmortem examination revealed a remarkable and bilaterall...
Source: Comparative Clinical Pathology - November 22, 2016 Category: Pathology Source Type: research

Assessment of impact of hemodialysis on hematological parameters among patients with chronic kidney disease
The objectives of this study was to find the changes in the complete blood counts, red blood cell indices, and peripheral blood smear in post hemodialysis patients and to compare the changes with respect to pre-dialysis status. We conducted a hospital-based cross-sectional study for a period of 3  years and a total of 120 patients undergoing dialysis. Blood samples were collected before and within 2 h post dialysis; complete hemogram and peripheral smear examination results were recorded and compared by Studentt test. There was moderate degree of anemia in both pre- and post dialysis patients, and also, there was...
Source: Comparative Clinical Pathology - November 22, 2016 Category: Pathology Source Type: research

Cytotoxic and genotoxic effects of the trypanocidal drug diminazene aceturate
AbstractDiminazene aceturate (D.A.) is an anti-parasitic drug that selectively targets adenine and thymine bases of deoxyribonucleic acid (DNA) of trypanosomes; however, there are some concerns regarding as its ability to induce DNA damage. Taking all of these into account, the aim of this study was to verify any possible toxic effects on the DNA of rats 7 and 21 post-injection (PI). All animals have received the therapeutic dose (single dose of 3.5  mg kg−1) intramuscularly. Liver, kidney, and total blood samples were collected to perform the analyses. The results showed increased (p 
Source: Comparative Clinical Pathology - November 18, 2016 Category: Pathology Source Type: research

Comparing hematological coagulation and biochemical parameters of healthy dogs living at below and above sea levels
AbstractThere is limited information in the literature about the effect of altitude on the variability of hematological, coagulation, and biochemical parameters in healthy dogs. The main aim of this research is to evaluate and compare hematological, coagulation, and selected biochemical parameters of apparently healthy dogs living below and above sea level. Hematological coagulation and selected biochemical parameters were compared between 21 apparently healthy middle-aged dogs living at 180 to 260  m below sea level and 1012 to 1160 m above sea level. The mean values of WBC, MCV, MCH, granulocytes, AST, BUN, sod...
Source: Comparative Clinical Pathology - November 18, 2016 Category: Pathology Source Type: research

Detection of pancoronavirus using PCR in Camelus dromedarius in Iran (first report)
AbstractCoronaviride is a colossal family of viruses that cause a variety of diseases in humans and other animals. As of late, a novel coronavirus, not anterior-optically discerned in humans, has been identified in a denizen of the Middle East. There is growing evidence that theCamelus dromedarius is host species for the virus and plays an important role of a source of human infection. Along these lines, the authors decided to detect coronaviruses in dromedary camels in two high-risk areas of Iran by employing an reverse transcription polymerase chain reaction (RT-PCR) assay. In the present study, nasal swab specimens were...
Source: Comparative Clinical Pathology - November 18, 2016 Category: Pathology Source Type: research

The prevalence and control of bovine mastitis in Leptospira outbreak
AbstractLeptospirosis is considered as one of the most widespread zoonotic disease among many species of wild and domesticated animals, including dairy cows worldwide. Due to its presence and proliferation capacity in the lactating mammary gland, leptospires-related mastitis and milk drop syndrome occur in lactating dairy cows. The aims of this study were to evaluate 1) the occurrence of mastitis due to leptospirosis and 2) the differences ofLeptospira mastitis occurrence between primiparous and multiparous cows. Leptospirosis occurred in a large industrial dairy cattle herd complex in the northwest of Iran for 2  yea...
Source: Comparative Clinical Pathology - November 12, 2016 Category: Pathology Source Type: research

Cupressus sempervirens extract inhibited human basal cell carcinoma tumorigenesis, local invasion, and angiogenic property
AbstractBasal cell carcinoma, a noninvasive and rarely metastatic tumor, correlates  with clinical and histological involvement of basal epithelial cells. It occurs due to dysregulation of hedgehog-patched1 signaling pathway. The current study was conducted to evaluate the in vitro cytotoxic effects ofCupressus sempervirens methanolic extract against primary basal cell carcinoma cells, over a period of 48  h. We measured protein level of Annexin-V and the leakage rate of lactate dehydrogenase in cells being exposed to 420 μM extract. In addition to transcript levels ofPTCH-1,  a recept...
Source: Comparative Clinical Pathology - November 12, 2016 Category: Pathology Source Type: research

Capillary refill time, bleeding time, clotting time, erythrocyte sedimentation rate and prothrombin time in natural cases of canine Trypanosoma congolense infection
AbstractTrypanosomiasis of man and his domestic animals continues to pose enormous public health and economic problems in many parts of Africa and South America. Thirty-one dogs were used for this study. 23 of the dogs were apparently healthy while eight were confirmed to be suffering fromTrypanosoma congolense. Confirmation of trypanosomiasis was done by wet mount and by the use of Dip Quick ™ stain. Capillary refill time, bleeding time, clotting time, erythrocyte sedimentation rate and prothrombin time were determined. The mean values for the apparently healthy dogs were 1.32 s (seconds), 3.34 min (minute...
Source: Comparative Clinical Pathology - November 12, 2016 Category: Pathology Source Type: research

Bilateral ovarian pedicle ligation as an alternative to ovariectomy and ovarian response to eCG treatment
In conclusion, the results of the present study showed that despite the severe changes in ovarian tissue following ovarian pedicle ligation, minor perfusions still exist and supply the ovaries so that hormone response and follicular development can occur. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 10, 2016 Category: Pathology Source Type: research

Retraction note to: Prevalence of the C282Y and H63D mutations of the HFE hemochromatosis gene in Azerian major β-thalassemia and iron overload
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 4, 2016 Category: Pathology Source Type: research

Second-degree atrioventricular block in a diarrheic calf-camel ( Camelus dromedarius )
AbstractAtrioventricular block (AVB) is a type of heart block in which the conduction between the atria and ventricles of the heart is impaired. The purpose of the present paper is to report the occurrence of second-degree AVB in a diarrheic calf-camel. A 7-day-old female calf-camel (Camelus dromedarius) weighting approximately 50  kg was referred to the veterinary hospital with a 5-day history of diarrhea, prostration, tremors, sternal recumbency, and muscular weakness. Clinical examination revealed increases in rectal temperature (RT 39.9 °C), heart rate (HR 124 beats min−1), and respiratory rate (RR ...
Source: Comparative Clinical Pathology - November 4, 2016 Category: Pathology Source Type: research

Clinicopathological and microbiological findings associated with wounds in Nigerian horses
This study was a 6-month survey of wound infections of horses at the Obollo-Afor Horse Lairage, Udenu Local Government area, Enugu State, Nigeria. Two hundred seven horses were sampled. They were physically examined, and those that had wounds were purposively selected for further study. Blood samples were collected from these horses that had wounds for haematological and serum biochemistry evaluations. Sterile swab sticks were used to collect samples from the wound edges after disinfection for microbiological examination. This showed that out of the 207 horses sampled, 21 had wounds. The horses with wounds had significantly (p 
Source: Comparative Clinical Pathology - November 4, 2016 Category: Pathology Source Type: research

Hematological and biochemical values in Brazilian four-beat gaited horses
AbstractHorse selection is still associated with gait type, and this aspect is important for four-beat gaited horses, a large group that is distributed around the globe with more than 140 breeds. However, there is little information about their hematological and biochemical profiles, and such information will contribute to improving their exercise and nutritional evaluation programs. The aim of this study was to determine the hematological and biochemical values of athletic Campolina (CAMP) and Mangalarga Marchador (MM) horses performing two different types of gait. Overall, 100 adult horses (53 males and 47 females) with ...
Source: Comparative Clinical Pathology - November 3, 2016 Category: Pathology Source Type: research

Evaluation of the correlation of automated and manual results of complete blood count in oncologic patients
AbstractThe aim of this study is to evaluate the performance of Sysmex XS-800i analyzer and compare its correlation with standard manual method in oncologic patients. The complete blood count (CBC) with differential leukocyte count is one of the most frequently requested hematologic tests. In oncologic patients, it is more important because primary diagnosis and treatment follow-up are depending on CBC results. Automated hematology analyzers are designed to perform CBC with acceptable precision and accuracy, but manual slide examination still is needed to complement the analyzer results. One thousand CBC samples of oncolog...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Effect of human amniotic membrane on anastomosis leakage in dog model
AbstractOne of the major concerns in rectal cancer surgery and complicated anastomosis is anastomosis breakdown and leakage. For decades, radiation (as neoadjuvant or adjuv ant therapy) was known as a risk factor for anastomosis leakage. Diverting loop ileostomy has been usually employed to divert the fecal flow and protect complicated anastomosis. However, there is a high rate of complications related to diverting loop ileostomy and its closure. This experimental study was carried out to investigate the effect of human amniotic membrane on colonic anastomosis in dogs that underwent preoperative radiation therapy. In this ...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Antimicrobial effects of new designed 2-amino-tetrahydro-4 H -chromene-3-carbonitrile derivatives against some pathogenic microbial strains
AbstractChromenes, also called benzopyrane derivatives and chromene-4-one, are natural compounds which have several biological effects. The aims of the present study were to synthesize 2-amino-tetrahydro-4H-chromene-3-carbonitrile derivatives and to evaluate their antibacterial effects against selected bacterial strains. Nine 2-amino-tetrahydro-4H-chromene-3-carbonitrile derivatives were designed and synthesized. Each synthesized derivative was dissolved in dimethyl sulfoxide and diluted using distilled water. Then, serial dilutions of 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, and 0.125  μg/ml were prepared and added to t...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

A case of feline panleukopenia in Felis silvestris in Iran; confirmed by PCR
In conclusion, current case report presents FPL inFelis silvestris for the first time in Iran. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Effect of Citrullus vulgaris on some functional indices of selected tissues of Wistar rats
This study aimed at evaluating the implications of such consumption. Study involved the use of 35 Wistar rats grouped into seven with a control group administered distilled water, and the other groups administered varying concentrations of the pulp and whole fruit for 28  days. Alanine and aspartate aminotransferase (ALT, AST) were assayed in the serum and liver. Total protein of the serum and selected tissues was determined and malondialdehyde (MDA) level measured. Phytochemical screening revealed the presence of steroids, saponin, alkaloids, flavonoids, and terpe noids in both whole fruit and pulp. Significant increases (p 
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Slaughterhouse report of intrahepatic cholangiocarcinoma in a Holstein cow
AbstractCholangiocarcinoma (CCA) is a primary tumor of the bile duct epithelium. Cholangiocarcinoma is relatively uncommon in domestic animals and the previous reports are documented more in dogs and cats, and less in other species. The current study described intrahepatic CCA in a 6-year-old female Holstein cow. Grossly, multiple parenchyma were scattered throughout the liver parenchyma. Histopathologically, the tumoral masses were composed of gland-like, tubular, acinar structures or solitary islands that embedded in the abundant connective tissue stroma with a cuboidal to columnar epithelial cells. Polymorphism and mito...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research