Phytochemical screening and toxicity investigation of hydro-methanolic and aqueous extracts from aerial parts of Sonchus oleraceus L. in Swiss albino mice

AbstractSonchus oleraceus L. is a Mediterranean leafy vegetable. The aerial parts are widely used in Algerian traditional medicine to treat several diseases such as inflammation, hepatitis, and for detoxication. To date, there are no studies corroborating its toxicity profile. This work aimed to investigate the qualitative phytochemical screening (QPS) and to evaluate the toxicity of the hydro-methanolic (HME) and hot aqueous (HAE) extracts of the aerial parts ofS. oleraceus L. in female mice. The QPS of both extracts was achieved using simple tests, as well as the acute and subacute toxicities were examined following Lorke ’s method and OECD guidelines 407, respectively. The qualitative phytochemical analysis revealed the presence of polyphenols, flavonoids, sterols and triterpenes, anthraquinone glycoside, and reducing compounds in both extracts. The administered doses of the two extracts disclosed no mortality and no visible sign of toxicity. The LD50 was estimated above 5  g/kg body weight. In the subacute test, the extracts did not induce neither mortality nor significant changes in body weight and relative organ weights. The data also revealed that HME leads to a disturbance in LDL, indirect bilirubin, while HAE caused a disorder in cholesterol, ALT, urea, creatin ine, and total proteins levels. They caused as well a strong disturbance of hematological parameters. Histopathological examination showed an important hepatic, renal, and lung lesions up to the dose of 200...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research