Phlogacanthus thyrsiflorus Nees. modulates hepatic and renal apoptosis via attenuation of oxidative stress in alloxan-administered mice

AbstractAn endeavor has been made in this study to assess the effect ofPhlogacanthus thyrsiflorus on hyperglycemia-induced oxidative stress along with apoptosis in liver  and kidney of alloxan-administered mice. To analyze the effect ofPhlogacanthus thyrsiflorus on oxidative stress in kidney  and liver of diabetic mice. Furthermore, we have also examined the effect ofPhlogacanthus thyrsiflorus on apoptosis in diabetic mice. Following preparation of methanolic flower extract (MFE), preliminary phytochemical screenings and acute toxicity test were carried out for MFE. Lipid peroxidation and protein carbonyl assays were determined to check the MDA level and oxidative damage in tissues of groups normal control mice (NCM), diabetic control mice (DCM), ascorbic acid –treated diabetic mice (D + AA), and MFE-treated diabetic mice (D + MFE). Histological and ultrastructural studies were conducted to evaluate any changes in tissues as well as sub-cellular organelles. The effects of MFE on caspase 3 and Bcl-2 expression in alloxan-induced diabetic mice wer e studied and compared against the diabetic control group of mice. Upon treatment with MFE, the diabetic mice manifested a notable depletion of malondialdehyde (MDA) and protein carbonyl levels. The ultrastructural studies divulged the capability of MFE to reinstate morphological and cellular altera tions as contemplated in alloxan-induced diabetic mice. On apoptosis, the effect of MFE showed the downregulation of cystei...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research