Combination of diminazene aceturate and resveratrol reduces the toxic effects of chemotherapy in treating Trypanosoma evansi infection
Abstract The aim of this study was to evaluate the effects of resveratrol (RSV) in its free or complexed (cyclodextrin) forms in association with diminazene aceturate (DA), as an attempt to reduce the toxic effects of this standard chemotherapy on renal and hepatic samples of mice experimentally infected with T. evansi. In this sense, this experiment was designed in order to test the ability of RSV, in its free or complexed forms, on reducing the damage on lipid membrane and protein oxidation, effects usually observed by using DA. Both forms of RSV were used at 100 mg kg−1, during seven consecutive...
Source: Comparative Clinical Pathology - July 11, 2015 Category: Pathology Source Type: research

The effects of aqueous extracts of Lippia citriodora , Trachyspermum copticum , Dracocepalum polychaetum on repair of ethanol induced gastric ulcer in rats
Abstract To evaluate the effects of Lippia citriodora, Trachyspermum copticum, and Dracocepalum polychaetum on healing of gastric ulcer induced by ethanol, 25 male Sprague–Dawley rats were divided into five groups (five rats in each group). After 24-h starvation, all animals received oral ethanol (1 mL/200 g body weight). Three groups received 2 mL (100 mg/kg/day) of L. citriodora (LC group), D. polychaetum (DP group), and T. copticum (TC group) aqueous extracts; one group (RA) received ranitidine (200 mg/kg/day); and one group (control) received 2 mL distilled water saline by g...
Source: Comparative Clinical Pathology - July 8, 2015 Category: Pathology Source Type: research

Protection by intraperitoneal administration of bone marrow-derived stem cells of lipopolysaccharide-induced brain and liver damage in mice
Abstract Stem cells induce functional improvement in experimental and clinical studies, but its mechanism is still not known. This paper studies the effect of a single systemic injection of bone marrow-derived stem cell (BM-SC) suspension on brain oxidative stress and on pathological changes induced in the brain and liver of mice by lipopolysaccharide (LPS) endotoxin. Moreover, the possible cholinergic modulation of stem cell effects was also studied. Mice were treated with intraperitoneal (i.p.) injection of stem cell suspension or saline with or without subcutaneous atropine (1 mg/kg) at time of LPS (200&nb...
Source: Comparative Clinical Pathology - July 7, 2015 Category: Pathology Source Type: research

Comparative assessment of erythrocyte osmotic fragility in two breeds of dog: Nigerian indigenous breed and the German shepherd
Abstract Haematological analyses are important in early detection of diseases, and breed differences have been suggested (Schalm 1962). This work is a comparative study to establish the influence of breed on erythrocyte osmotic fragility of Nigerian indigenous breed of dog and the German shepherd dog. In the study, erythrocyte values and the erythrocyte osmotic fragility of Nigerian indigenous breed of dog were compared with those of the German shepherd dog. The influence of sex on the erythrocyte values of the German shepherd dog was also investigated. There were no significant differences (P 
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

The antipsychotic drug sulpiride induces insulin intolerance but does not affect body weight in adult male mice
Abstract Research studies have shown that chronic administration of some antipsychotic drugs induces obesity in female but not in male. To explore the mechanisms involved in this effect, we administrated the dopamine antagonist sulpiride (40 mg/kg) or vehicle intraperitoneally to adult male mice during 45 days and then the glucose tolerance and serum levels of insulin, serum, and testicular testosterone and prolactin, and blood estrogen levels were evaluated. Chronic sulpiride administration did not affect body weight. However, prolactin levels and the area under the glucose and insulin curves were signi...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

Aelurostrongylus abstrusus in a stray cat’s blood stream
Abstract Aelurostrongylus abstrusus (Raillet), the cat lungworm, is a parasitic round worm which lives in the terminal bronchioles and the alveolar ducts inside the lungs of cats as final hosts. Snails or slugs can be intermediate hosts while rodents, frogs, toads, lizards, birds, and snakes can play the role of transporter from the intermediate host into the final host. The ingested third-stage larvae migrate towards the lungs through the blood vessels. In this research, first, the observation of A. abstrusus infection in a stray cat in Iran is described. Later, the larval detection of this worm ...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

Effects of two doses of tramadol on pain and some biochemical parameters in rabbits post-gastrotomy
This study was conducted to evaluate the effect of two doses of tramadol on pain and some biochemical parameters in rabbits post-gastrotomy. Fifteen 6-month-old male rabbits were used for the study. The animals were randomly assigned into three groups of five rabbits each. Physiological (heart rate [HR], respiratory rate [RR], body weight [bw], temperature, blood glucose and serum cortisol) and biochemical (alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], blood urea nitrogen [BUN] and creatinine) parameters of all the animals were determined and recorded as the baseline values. ...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

Effect of local administration of fibroblastic growth factor with chitosan conduit on peripheral nerve regeneration: a rat sciatic nerve transection model
Abstract Technological advances in diagnostic imaging, neurosurgical instrumentation, and the use of a surgical microscope have resulted in pronounced improvements in the diagnosis and repair of transected peripheral nerves. Effect of local fibroblast growth factor (FGF) on nerve regeneration was assessed. Eighty male Wistar rats were divided into four experimental groups (n = 20), randomly: In transected group, left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group, defect was bridged using a chitosan conduit filled with 10 μL FGF. In chitosan conduit...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

Changes in serum levels of purines in an experimental infection by Leptospira interrogans serovar Icterohaemorrhagiae
Abstract The aim of this study was to measure the levels of purines in serum of rats experimentally infected with Leptospira interrogans serovar Icterohaemorrhagiae. For this purpose, 24 adult male Wistar rats were divided into two groups: A, not infected (subgroups A1 and A2), and B, infected (subgroups B1 and B2), which were intraperitoneally inoculated (day 0) with 2 × 108 leptospires/animal. Blood samples were collected on days 15 (A1 and B1) and 30 PI (A2 and B2) for analysis of purine levels in serum (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

The Arabian camel ( Camelus dromedarius ) as a major reservoir of Q fever in Saudi Arabia
Abstract Serum samples from 489 male and female camels were tested for antibodies against Coxiella burnetii using indirect enzyme-linked immunosorbent assay (ELISA). Antibodies to C. burnetii were recorded in sera of 252 (51.64 %) camels. Significant differences in prevalence were found between male and female camels, juvenile and adult camels, and different ecotypes and different sampling locations. Three hundred seven camels were simultaneously tested for C. burnetii antibodies by ELISA and indirect immunofluorescence (IFA) tests. Close agreement was found between the results of the two tests. A high preva...
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

Benign multicystic peritoneal mesothelioma associated with partial spontaneous regression in an aged cow
This article describes a benign multicystic peritoneal mesothelioma presenting partial spontaneous regression in a 16-year-old Japanese Shorthorn cow, which exhibited innumerable fluid-filled cysts (
Source: Comparative Clinical Pathology - July 1, 2015 Category: Pathology Source Type: research

First report of Ophidascaris filaria (Nematode: Ascarididae) from the Indian python in Sistan, Iran
This study summarizes the results of the necropsy of a python found dead in a zoo in Sistan, Iran. Two nematodes, ultimately identified as Ophidascaris sp., were extracted from the python’s body. Nematode of Ascarididae family, Ophidascaris sp., is principally found in pythonid and colubrid snakes. Nematodes often cause gastrointestinal, hepatic, and pulmonary lesions. Ascarid nematoda is an important pathogen for snakes, and infestation can be fatal. In this study, the Ophidascaris filaria species was isolated from an Indian python (Python molurus), in Sistan. To the author’s knowledge, O. filaria is recorded ...
Source: Comparative Clinical Pathology - June 30, 2015 Category: Pathology Source Type: research

Which quantitative method in determination of the thyroid hormone levels is more consistent with the clinical symptoms of the thyroid disorders?
Abstract Various methods have been used to determine thyroid-stimulating hormone (TSH) and thyroid hormone concentrations in medical diagnostic laboratories. Selection of a suitable method for diagnosis and monitoring thyroid disorders is necessary. The study aimed to compare chemiluminescent assay (CLA), radioimmunoassay (RIA), and enzyme-linked immunosorbent assay (ELISA) methods for the determination of thyroid hormone levels in human serum. Blood samples were taken from 137 patients with thyroid disease and 58 healthy subjects. The sera were analyzed simultaneously to determine the concentration of TSH, thyrox...
Source: Comparative Clinical Pathology - June 30, 2015 Category: Pathology Source Type: research

Effects of methanolic fruit extract of Solanum macrocarpum L. (Solanaceae) on biochemical profile of albino rats ( Rattus norvegicus )
This study suggests that methanolic fruit extract of S. macrocarpum may be useful in reducing serum cholesterol levels. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 30, 2015 Category: Pathology Source Type: research

Letter to the editor: Theileria in loggerhead sea turtles Caretta caretta
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 30, 2015 Category: Pathology Source Type: research

Infectious bursal disease vaccine ameliorates velogenic Newcastle disease virus infection in immunopotentiated chickens
In conclusion, the immunopotentiation of the chickens as well as NDV and IBD vaccination improved the bird immunity and diminished the immunosuppressive effect of the vvNDV. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 24, 2015 Category: Pathology Source Type: research

Silver nanoparticles affects the expression of biomarker genes mRNA in rainbow trout ( Oncorhynchus mykiss )
Abstract Due to their strong antiseptic properties, silver nanoparticles (AgNPs) are widely used in consumer products, and therefore there is a high risk of their release to the aquatic environment. In the present study, we investigated chronic effect of waterborne AgNPs on the mRNA expression of four important biomarker genes including metallothionein (MT), glutathione S-transferase (GST), superoxide dismutase (SOD), and glutathione peroxidase (GPX) in the gill tissue of rainbow trout. Twenty-one-day exposure to AgNPs significantly increased MT and GST mRNA levels in the gills in a dose-dependent manner. Although...
Source: Comparative Clinical Pathology - June 24, 2015 Category: Pathology Source Type: research

Assessment of the antimicrobial activity of Melissa officinalis and Lawsonia inermis extracts against some bacterial pathogens
Abstract The postburn infections especially antibiotic-resistant bacterial infections have been recognized as a significant and major public health problem. Staphylococcus aureus, Escherichia coli, Acinetobacter, Klebsiella pneumoniae, and Pseudomonas aeruginosa are among very important bacterial pathogens isolated from postburn infections. The present study has evaluated the antimicrobial activity of some plant extracts (Melissa officinalis and Lawsonia inermis) against some bacterial pathogens causing burn wound infection. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC...
Source: Comparative Clinical Pathology - June 24, 2015 Category: Pathology Source Type: research

Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis -infected mice and its lipopolysaccharides (LPSs)
Abstract Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis-infected mice and its lipopolysaccharide (LPS) were investigated. Forty-eight mice (24 males and 24 females) were used as infection models in this study, and they were divided into three equal separate groups of eight mice based on their sex: M1, M2, and M3 and F1, F2, and F3 (M = Male, F = Female). Groups M1 and F1 were orally inoculated with sterile phosphate-buffered saline (PBS) and served as the negative control. Groups M2 and F2 were orally inoculated with 0.4 ml of 109 colony-forming...
Source: Comparative Clinical Pathology - June 19, 2015 Category: Pathology Source Type: research

Serum bile acids concentration in captive black-tailed prairie dogs ( Cynomys ludovicianus )
This study was conducted in order to determine serum bile acids concentration in captive black-tailed prairie dogs (Cynomys ludovicianus). Sixteen healthy intact prairie dogs of both sexes were studied as part of an overall clinical health evaluation performed under general isoflurane anesthesia. Each animal underwent a complete physical examination, complete blood count, serum biochemistry, urinalysis, whole body radiology, and cardiographic examination. Blood samples were collected from the Jugular vein and submitted for a serum bile acids analysis. The mean and median serum bile acids concentration for the black-tailed ...
Source: Comparative Clinical Pathology - June 17, 2015 Category: Pathology Source Type: research

Mixed tumor of apocrine sweat gland in a terrier dog
Abstract A 4-year-old, female terrier dog was presented with complaint of a solid mass found incidentally on the left groin area. Touch of mass was painless. The skin overlying the mass was normal in appearance, with no ulceration. Complete physical examination, hematological tests, and radiology were done. No other abnormality was detected in clinical examination, and laboratory findings were within normal ranges. Radiographic surveys showed no local and distal metastasis. With excisional biopsy, the whole abnormal area was removed. It was loosely attached to the adjacent tissues. Grossly, the lesion was a 2.5&th...
Source: Comparative Clinical Pathology - June 17, 2015 Category: Pathology Source Type: research

Alterations in atherogenic indices and hypolipidemic effect of soybean oil in normocholesteremic rats
Abstract The cardiovascular protective and hypolipidemia effects of soybean oil were investigated in normocholesteremic rats. Diets were prepared and designed to contain 50 % of energy as carbohydrate, 35 % as fat, and 15 % as protein. Groups of six rats were each fed one of these diets, while a group was fed pelletized mouse chow which served as the control. A significant reduction (p 
Source: Comparative Clinical Pathology - June 17, 2015 Category: Pathology Source Type: research

Radiological and histological comparison between nano - bioglass and commercial bioglass in bone healing in a rabbit model
In this study, we evaluated and compared the effects of nano-bioglass and commercial bioglass on healing of experimental tibial defects in ten white New Zealand rabbits. Three round holes were created in tibial bone with orthopedic drill. One of the hole was left empty as control while the other two holes were filled by experimental grafts. Radiografts were taken after 2 and 6 weeks whereas pathological slides were prepared only after 6 weeks. Radiological and histological evaluation was performed blindly, and the results score were analyzed statistically. The data showed that nano-bioglass is superior to commercial biogla...
Source: Comparative Clinical Pathology - June 13, 2015 Category: Pathology Source Type: research

Post challenge variability in time gap pertaining to appearance of Toxoplasma gondii DNA in blood and appearance of visible clinical signs in murine model
In conclusion, ample time gap was found regarding the initiation of clinical infection and actual appearance of visible clinical signs that could prove detrimental in final outcome of condition. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 12, 2015 Category: Pathology Source Type: research

Immunophenotype, microRNA expression and cytogenetic characterization of acute leukemias of ambiguous lineage
Abstract Acute leukemias of ambiguous lineage (ALAL) form a group of leukemias presenting the features of both acute lymphoid leukemia (ALL) and acute myeloid leukemia (AML). Based on Immunologic Classification of Leukemia (EGIL), these leukemias are recognized as biphenotype acute leukemia (BAL). According to WHO classification and based on immunophenotypic characteristics, ALAL is further categorized to acute undetermined leukemia (AUL), mixed phenotype acute leukemia (MPAL), and other ambiguous lineage leukemias. Immunophenotype and cytogenetic aspects are important in recognition of these leukemias; however, t...
Source: Comparative Clinical Pathology - June 8, 2015 Category: Pathology Source Type: research

Diagnostic accuracy of dual-source CT angiography for evaluation of coronary artery and comparative analysis of the DSCT angiography of the internal carotid artery plaque with the histopathological specimens
Abstract The purpose of this study was to investigate the diagnostic accuracy of a dual-source CT (DSCT) coronary angiography in parallel with a CT angiography. On the other hand, we compared the DSCT angiography of the internal carotid artery plaque with the histopathological specimens. Ninety patients underwent DSCT and an invasive coronary angiography (ICA). All segments were analyzed at 60 and 70 % of R-R interval initially. After finding the reconstruction interval, image quality was divided for each coronary segment on the four-point Likert scale. Also, of these patients, 30 cases that had neurological ...
Source: Comparative Clinical Pathology - May 23, 2015 Category: Pathology Source Type: research

Haematologic profile and histomorphologic assessment of the pancreas and liver of alloxan-induced diabetic rats treated with graded concentration of quail egg
This study was designed to investigate the effect of quail egg on the haematological parameters and the histopathology changes of the pancreas and liver of alloxan-induced diabetic Wistar albino male rats. Thirty albino rats with a mean weight of 160 ± 15 g were randomly selected into five groups, each with six rats. Groups 2, 3, 4 and 5 were induced with diabetes by intraperitoneal injection of alloxan at a dose of 160 mg/kg. Upon confirmation of diabetes (fasting blood sugar (FBS)>126 mg/dl), rats in groups 2, 3 and 4 were orally administered with 30, 15 and 7.5 mg/ml of quail ...
Source: Comparative Clinical Pathology - May 21, 2015 Category: Pathology Source Type: research

Stomach impaction and ascaridiasis in Himalayan Griffon vulture ( Gyps himalayensis ): a case study
This study involved an adult male Himalayan Griffon vulture, Gyps himalayensis, housed in Dhauladhar Nature Park (Zoo), Gopalpur, Himachal Pradesh, India. The bird manifested anorexia, emaciation, scant defecation, and reluctance to move. Despite supportive medication, it died. On post mortem examination, the proventriculus and gizzard were found to be firmly distended due to a solid mass made up of long grass material (phytobezoar). The accumulated grass material had led to occlusion of the gastric passage. A heavy infestation of roundworms of Ascaris spp. was also noted in the small intestine. The histopathological exami...
Source: Comparative Clinical Pathology - May 21, 2015 Category: Pathology Source Type: research

Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium
Abstract The aim of the present study was to determine the prevalence of porcine circovirus type 2 (PCV2) DNA-positive uterine tissues in gilts in relation to endometritis and the number of leukocyte subsets in the endometrium. Uterine tissues from 32 Landrace × Yorkshire gilts were investigated. The gilts were classified according to both macroscopic and microscopic examination of the endometrium into three groups: normal (n = 13), acute endometritis (n = 11) and chronic endometritis (n = 8). Reproductive cycle was defined according to the ovarian appearance as foll...
Source: Comparative Clinical Pathology - May 21, 2015 Category: Pathology Source Type: research

Nigella sativa supplementation attenuates exercise-induced bronchoconstriction in the maturing rat: a histometric and histologic study
Abstract Recent evidence suggests that Nigella sativa (NS) has an antiasthmatic (bronchodilatory) effect in the airways of asthmatic patients. Our objective was investigation of the hypothesis that NS supplementation reduces exercise-induced bronchoconstriction (EIB) in the maturing rat after high-intensity interval training (HIIT). Forty male Wistar rats (aged 3 weeks) participated in this study. The animals after 2-week adaptation were divided into three groups: Base, control HIIT, and HIIT (HIIT and NS group). HIIT group was performed on a treadmill (20 to 70 m/min, 30 min/day, 6 days/w...
Source: Comparative Clinical Pathology - May 21, 2015 Category: Pathology Source Type: research

Effects of sex and age on the osmotic stability of Sahel goat erythrocytes
Abstract Adaptation of goats to the arid and semi-arid environments may involve adjustments in erythrocyte osmoregulation. Sexual dimorphic and age-related effects on osmotic stability of the goat erythrocyte were investigated to determine physiological variations. Apparently healthy male (n = 23) and non-pregnant dry female (n = 24) Sahel goats, aged 1½–2,>2–2½, and>2½ years with mean body weights of 10.35–27.21 kg, were selected for the study from a semi-intensively managed flock. Heparinised venous blood of each goat was used t...
Source: Comparative Clinical Pathology - May 19, 2015 Category: Pathology Source Type: research

Gastroprotective effect of aqueous extracts of Lippia citriodora , ajowan ( Trachyspermum copticum ), and Dracocepalum polychaetum on induced gastric ulcer in rats
Abstract In the present study, the gastroprotective effects of Lippia citriodora, Trachyspermum copticum, and Dracocepalum polychaetum on gastric ulcer induced by ethanol were investigated. Twenty-five male Sprague–Dawley rats were divided into five groups (five rats in each group). Three groups received 100 mg/kg/day (2 ml) of L. citriodora (LC group), D. polychaetum (DP group), and T. copticum (TC group) aqueous extracts, one group (RA) received ranitidine (200 mg/kg/day), and one group (control) received 2 ml distilled water saline by gastric gavage for four consecutive days. On the 4t...
Source: Comparative Clinical Pathology - May 15, 2015 Category: Pathology Source Type: research

Detection of paratuberculosis using histopathology, immunohistochemistry, and ELISA in West Algeria
Abstract Paratuberculosis (Ptb), caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic enteritis that affects many ruminants and other wild animals worldwide. Although the prevalence of infection is unknown in nearly all countries, many studies report that paratuberculosis is the greatest economic problem in animal health. Here, we report the occurrence of paratuberculosis in herds of cattle in the Tiaret region of Algeria, using different diagnostic procedures. Tiaret and Sougueur abattoirs were being selected representing the main parts of the province. Four hundred intestines were examined f...
Source: Comparative Clinical Pathology - May 15, 2015 Category: Pathology Source Type: research

Aspartate aminotransferase to platelet ratio index and severity of hepatic fibrosis in children
This study aims to investigate the association between APRI value and severity of liver fibrosis. Blood samples were taken from 52 patients with the age of 2 months to 18 years who were referred to the Pediatric Gastroenterology Ward of Nemazee Teaching Hospital with diagnosis of chronic liver disease. The aspartate aminotransferase (AST) level and platelet counts 1 week before the taking of liver biopsy were determined. Closed biopsy of the liver through the skin was performed, and the severity of hepatic fibrosis was interpreted according to the Meta-analysis of Histological Data in Viral Hepatitis (METAVI...
Source: Comparative Clinical Pathology - May 12, 2015 Category: Pathology Source Type: research

Surgical management of concurrent histiocytoma and dermatophytosis in an 8-month-old male Boarboel dog
Abstract An 8-month-old male Boarboel dog was presented with a mass on the rostral aspect of the face which bled consistently. The patient also had areas of alopecia on the left hind limb, right forelimb, head, and abdominal regions. The mass progressively increased in size with time without regression. Radiographic examination of the chest showed no evidence of metastasis. Cultures of the skin swab as well as that of the bleeding facial swelling yielded heavy growth of Staphylococcus sp. (+++) while the skin scraping yielded heavy growth of Microsporum canis. Microscopically, the growth was a hypercellular mass, ...
Source: Comparative Clinical Pathology - May 12, 2015 Category: Pathology Source Type: research

Glycine transporter of canine lens epithelial cells: functional analysis and molecular structure
Abstract Glycine transport activity in a canine lens epithelial cell line was investigated. The glycine transport activity was 2.70 ± 0.257 nmol/mg protein/min at 100 μM extracellular glycine concentration, which was reduced to 9 % in a sodium-free condition. ALX5407 (analogue of sarcosine), the specific inhibitor of the glycine transporter 1 (GlyT1), reduced 30 % of the transport activity at 100 nM; on the other hand, ALX1393, the glycine transporter 2 (GlyT2) inhibitor, did not affect glycine transport activity of lens epithelial cells. A full-length cDNA sequence...
Source: Comparative Clinical Pathology - May 10, 2015 Category: Pathology Source Type: research

Antioxidant and antidiabetic effects of gallic and protocatechuic acids: a structure–function perspective
This study sought to investigate antioxidant and antidiabetic effects of gallic acid (GA) and protocatechuic acids (PCAs) based on their structure–function relationship. Twenty micromolar of phenolic acid (GA and PCA) solutions was prepared and their antioxidant properties determined. Then, interaction of the phenolic acids with key enzymes linked to type 2 diabetes (α-amylase, α-glucosidase) was subsequently assessed. The results showed that both phenolic acids significantly (P 
Source: Comparative Clinical Pathology - May 10, 2015 Category: Pathology Source Type: research

Histopathological and immunohistochemical study of rat brain tissue after exposure to mobile phone radiation
Abstract The health effects of cell phone radiation exposure are a growing public health concern. The aim of this study was to investigate the effects of mobile phone exposure at 940-MHz frequency magnetic field on temporal cortex. The study was carried out on 30 Wistar adult male rats (15–20 weeks, weighing 290 g). Thirty male rats were divided into five groups. Rats in the first group were exposed to 940-MHz frequency magnetic field for 30 min every day for a period of 30 days. The second, third, and fourth groups were exposed to 940-MHz frequency for 1, 2, and 3 h every day for a ...
Source: Comparative Clinical Pathology - May 10, 2015 Category: Pathology Source Type: research

Histopathological evaluation of the osteogenic activity of autologous platelet-rich plasma in experimentally induced ulna defect in dogs
This study was designed to evaluate the histopathologic effects of autologous platelet-rich plasma and cancellous bone grafts on caudolateral ulna ostectomy of Nigerian local dogs. Twelve healthy Nigerian indigenous dogs (males) were used for the study. They were randomly grouped into four viz: platelet-rich plasma-treated group (PRPG) (n = 3), cancellous bone graft-treated group (CBG) (n = 3), platelet-rich plasma/cancellous bone graft-treated group (PRP/CBG) (n = 3), and normal saline-treated control group (CG) (n = 3). Caudolateral ulna defects were created on the ...
Source: Comparative Clinical Pathology - May 8, 2015 Category: Pathology Source Type: research

The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia
Abstract Paravertebral block is a method of regional block used to make pain relief and decrease systemic analgesic requirements for patients undergoing breast cancer surgery. This work aimed to evaluate the effect of paravertebral block on monocyte phenotype and their functions after surgery for cancer breast patients. One hundred patients which the American Society of Anesthesia (ASA) I–III planned for breast cancer surgery were allocated into two groups. Group A (n = 50) received general anesthesia in a standard technique. Group B (n = 50) received paravertebral block T2–...
Source: Comparative Clinical Pathology - May 8, 2015 Category: Pathology Source Type: research

Immunohistochemical expression of manganese-superoxide dismutase (Mn-SOD) and triose-phosphate isomerase (TPI) in canine mammary gland tumor
Abstract Canine tumors, especially canine breast cancer, share common properties, including metastatic pattern, histopathological characteristics, and biological features with human cancers. Therefore, canine mammary gland tumor is deemed as an appropriate model for human breast cancer. We investigated expression of two important tumor antigens, including manganese superoxide dismutase (Mn-SOD) and triose-phosphate isomerase (TPI) in canine mammary gland tumors using immunohistochemistry analysis. Expression of these proteins were evaluated in tumor (29 malignant and 16 benign samples) and normal (n =&thins...
Source: Comparative Clinical Pathology - May 7, 2015 Category: Pathology Source Type: research

Effect of exercise on erythron, heart and respiratory rates in horses vaccinated against equine herpes virus 4/1 and equine influenza virus
Abstract Limited data are available for the influence of physical training in jumping horses at the background of booster vaccination. The aim of the study was to establish the effects of physical exercise applied at the background of booster vaccination against equine herpes virus 4/1 (EHV 4/1) and equine influenza virus (EIV) on changes in the erythron, breathing and pulse rates of horses. The differences were investigated between vaccinated and exercised horses and vaccinated-only controls. Fifteen Hanoverian geldings were distributed into 3 groups: group А, 3 non-vaccinated animals; group В, 6 vaccinated and...
Source: Comparative Clinical Pathology - May 6, 2015 Category: Pathology Source Type: research

Sub-acute toxicity of lantadenes isolated from Lantana camara leaves in guinea pig animal model
Abstract The sub-acute toxicity of lantadenes isolated from Lantana camara leaves was studied in guinea pig laboratory animal model. A total of 20 guinea pigs of either sex were divided into five groups. Group I served as control, while groups II, III, IV, and V were orally administered lantadenes at the dose rate of 100, 50, 25, and 12.5 mg/kg body weight (bw), respectively for 28 days. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, total bilirubin, blood urea nitrogen, and creatinine were estimated in serum. The effect of lantadenes on lipid ...
Source: Comparative Clinical Pathology - May 3, 2015 Category: Pathology Source Type: research

A serological survey on Borrelia burgdorferi infection among companion dogs in Ahvaz district, southwestern Iran
Abstract Canine Lyme borreliosis is one of the most common tick-transmitted diseases in the world. The infection is caused by spirochetal bacteria from the genus Borrelia burgdorferi. The principal vectors are various species of slow-feeding hard ticks of the Ixodes complex. Dogs are the most infected animals among the pets. The status of infection due to Borrelia in dogs remains unknown in many areas of Iran. Few studies have been reported on the distribution of this disease in the Iran dog’s population, so the aim of this survey was to evaluate the seroprevalence of Borrelia infection in companion dogs in ...
Source: Comparative Clinical Pathology - May 3, 2015 Category: Pathology Source Type: research

Antioxidant enzymes gene polymorphisms and hepatocellular carcinoma in hepatitis C virus-infected Egyptian patients
Abstract Oxidative stress is an imbalance between production and elimination of reactive metabolites of oxygen and nitrogen, in favor of their production leading to potential damage. During oxidative stress, biologically important molecules and cells can be damaged, and this can be significant in the pathogenesis of many diseases. Reactive oxygen species (ROS) and ROS-induced cytokines are known to trigger the apoptosis of some hepatocytes and therefore contribute to inflammation, regeneration, fibrogenesis, and carcinogenesis. The enzymes generally considered to be the frontline defense against ROS are catalase ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Baseline blood values for healthy free-ranging green sea turtles ( Chelonia mydas ) in Puerto Rico
We report packed cell volume, plasma biochemistry, and protein electrophoresis reference interval values for juvenile and subadult green sea turtles. Development of site-specific baseline blood health parameters for wild, healthy sea turtle populations is an important factor in creating effective management protocols and enhances our ability to understand the effects of anthropogenic and environmental changes on sea turtle health. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Prostatic carcinoma in a dog: a new approach by using the Gleason system
We describe the pathological features of prostatic carcinoma in a dog using the Gleason system where the related score was estimated as 7 (3 + 4). Perineural invasion was also noted, and the neoplastic cells showed no reaction with PSA antibody but were positive for Ki-67 and CK-7 and IHC staining. The expression of Ki-67-SI was 3.28 %. Traditional canine prostate cancer classification could not cover the obscured characteristics of our case; therefore, a more descriptive histological pattern system using the Gleason grading is recommended. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Serological study of toxoplasmosis in women with previous history of abortion at Hamedan’s medical centers during 2012–2013
Abstract Toxoplasma gondii is one of the major agents of infectious abortions and, due to its worldwide distribution, can threat healthy pregnant women who had no previous exposure to this parasite. The present study was designed to determine the titer of anti-toxoplasma antibody in women with history of abortion visiting Hamedan’s medical centers during 2012–2013. Blood samples were collected from 300 mothers with previous history of abortion coming to the provincial medical centers during the period spanning October 2012 to June 2013. The sera were isolated and subjected to evaluate the ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis
Abstract The aim of this study was to assess the pathophysiological importance of lipid profile, acute phase proteins, proinflammatory cytokines and oxidative stress markers in sheep with pneumonic pasteurellosis. Blood samples were collected from 36 Pasteurella multocida-infected sheep, together with 20 healthy controls. Samples for bacteriological examination (nasal swabs, bronchoalveolar lavage) were collected from all animals and subjected to bacteriological examinations. Moreover, heart blood and lung samples were collected from the dead pneumonic sheep and subjected also to bacteriological examinations. A l...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Comparative study of therapeutic protocols used in the treatment of infections caused by rickettsiales in dogs
Abstract Tick-borne diseases are caused by rickettsiales microorganisms of the genera Anaplasma and Ehrlichia, and often are diagnosed in dogs (Canis familiaris). Therapeutic protocols include antibiotics of different classes, mainly tetracyclines and derivatives. This comparative study contrasts two treatment protocols routinely used in veterinary medicine to treat Rickettsiosis and assesses the respective hematological changes. Dogs used for this research were divided in two groups: group 1, treated with long-acting oxytetracycline 15 mg kg−1 and group 2, treated with doxycycline 10 mg ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research