Comparison of efficacies of fetal bovine sera from different suppliers in cell culture experiments
AbstractFetal bovine serum (FBS) is widely used as a culture medium supplement. A high degree of variations is observed among FBS samples produced by different suppliers. The purpose of the present study was to compare between the quality and characteristics of FBS produced at Avicenna Research Institute (ARI-FBS) and a commercial FBS. Blood samples were taken from 50 healthy bovine fetuses at 2 –9 months of age. Following clotting of the blood, samples were centrifuged. Their sera were collected under sterile conditions. After irradiation, to investigate the performance and quality assurance of the serum sample...
Source: Comparative Clinical Pathology - December 29, 2017 Category: Pathology Source Type: research

Potential demographic, clinical, and environment risk factors for canine visceral leishmaniasis using IFAT and nested PCR, southeastern Iran
This study was performed between 2014 and 2015 on 210 randomly selected stray dogs which were caught by live traps. A 5  ml blood sample was taken from cephalic vein for indirect immunofluorescence antibody test (IFAT) and nested PCR. The cut-off titer for IFAT was 1:320. For molecular test, two sets of general and specific primers were used to amplify variable mini circle region of kinetoplast DNA (kDNA). Data anal yses were performed byx2 and logistic regression tests atP 
Source: Comparative Clinical Pathology - December 29, 2017 Category: Pathology Source Type: research

Prevalence of Listeria monocytogenes in cerebrospinal fluid obtained from hospitalized patients
AbstractThe aim of the present study was to determine the occurrence ofListeria monocytogenes in cerebrospinal fluid (CSF) samples obtained from patients with meningitis symptoms. A total of 102 CSF samples which were referred for bacterial culture to microbiology laboratory in Mashhad ’s Hospital were included in this study. All of the samples were cultured and positive ones confirmed by multiplex-PCR. Molecular serotyping and virulence characterization of isolates were performed. Random amplified polymorphic DNA-PCR using three set of primers was performed for evaluation of ge netic diversity of these isolates. Fiv...
Source: Comparative Clinical Pathology - December 23, 2017 Category: Pathology Source Type: research

Applications of fluorescence in situ hybridization in detection of disease biomarkers and personalized medicine
AbstractFluorescence in situ hybridization (FISH) method, as a molecular technique, is applicable for studying the gene expression during the cell differentiation, capturing images from the chromosomes/chromatin ’s areas in interphase, and detecting the chromosomal abnormality and rearrangements. This potential and impressive technique with high sensitivity and specificity could detect the biomarkers of various diseases. Therefore, it is very useful for accelerating therapy and enhancing the prognosis of the disease. A glimpse at this molecular technique and focus on its effective applications confirm it as a supreme...
Source: Comparative Clinical Pathology - December 22, 2017 Category: Pathology Source Type: research

Hematological parameters of pure and reciprocal crosses of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822)
AbstractComparative study on the hematological parameters of pure and reciprocal crosses of the African and Asian catfish was done to establish a reference range for fingerlings of the novel fish. Blood was collected fromClarias gariepinus (CG),Pangasianodon hypophthalmus (PH), Pangapinus ( ♀PH × ♂CG), and the two observed morphotypes (Clarias-like and Panga-like) of Clariothalmus (♀CG × ♂PH) obtained from similar breeding history. Result obtained revealed that red blood cell counts and white blood cell counts were significantly lower in Panga-like Clariothalmus (1.08  &...
Source: Comparative Clinical Pathology - December 21, 2017 Category: Pathology Source Type: research

High-intensity interval training-induced inflammation and airway narrowing of the lung parenchyma in male maturing rats
This study examined 6  weeks of HIIT-induced inflammation and airway narrowing of the lung parenchyma in male maturing rats. All animals after 2 weeks of familiarization were divided in 3 groups of base (n = 10), HIIT (n = 10), and control (n = 10). HIIT was performed at speed of 20 m/min and ended at speed of 70 m/min (incrementally increase). Twenty-four hours after, the last training session animal was sacrificed and their lung removed. Lung samples were sectioned in 5 μm, stained with H&E, and studied histologically and histometrically. All these...
Source: Comparative Clinical Pathology - December 21, 2017 Category: Pathology Source Type: research

Protective action of aqueous leaf extract of Gazania krebsiana (Less.) ‘Asteraceae’ antagonizes isoproterenol-triggered myocardial infarction in Rattus norvegicus
AbstractGazania krebsiana (Less.) is employed traditionally as treatment in the Basotho Traditional medicine in the management of numerous ailments. The study evaluated the cardioprotective action of aqueous leaves extracts ofG. krebsiana (ALEGK) against cardiotoxicity induced by isoproterenol hydrochloride (ISP). ISP (1  mL; 60 mg kg−1 BW) dissolved in 0.9% sodium chloride was used to induce myocardial damage intraperitoneally in the experimental animals. The outcomes of pre-treatment with 1  mL of the doses (125, 250, and 500 mg kg−1 BW) of ALEGK on consumed food, water intake, b...
Source: Comparative Clinical Pathology - December 20, 2017 Category: Pathology Source Type: research

Prodorsal liposarcoma in a gyr-saker hybrid falcon ( Falco rusticolus x Falco cherrug )
AbstractAn adult female gyr-saker hybrid falcon (Falco rusticolus x Falco cherrug) was brought to our falcon medical and research hospital for evaluation of a rapidly growing mass. Physical and radiographic examination revealed a large, oval-shaped, soft tissue mass on the left prodorsal region. Hematological findings showed moderate leukocytosis and heterophilia while the results of blood biochemical analyses were within normal ranges apart from a moderate elevation of creatine phosphokinase (CPK) activity. A cursory cytological evaluation of fine-needle aspirate of the mass revealed numerous RBCs while neither inflammato...
Source: Comparative Clinical Pathology - December 20, 2017 Category: Pathology Source Type: research

Rare case of primary thyroid lymphoma of the thyroid gland
We present a case of PTL diagnosed in a 63-year -old man in our hospital. The patient complained from a rapidly enlarging cervical mass associated with dyspnea for 5 months. On physical examination, the thyroid gland size was diffusely increased with a prominence of the right side. Cervical lymphadenopathy was not palpable. The patient underwent total thyroidectomy. Histomorphologic review of the excised thyroid and immunohistochemical studies confirmed diffuse large B cell lymphoma. The patient underwent systemic chemotherapy. The diagnosis of primary thyroid lymphoma should be considered when thyroid gland size show...
Source: Comparative Clinical Pathology - December 20, 2017 Category: Pathology Source Type: research

Association between TSLP gene polymorphism and bronchial asthma in children in Beni Suef Governorate in Egypt
The objectives of this study are to study the polymorphism of the two SNPs Rs2289276 and Rs2289278 in TSLP gene and to analyze any possible relation to pediatric bronchial asthma liability in Egypt using case-control study. DNA was extracted from blood samples withdrawn from 40 asthmatic patients and 20 age- and sex-matched normal controls, then the genotypes of SNPs Rs2289276 and Rs2289278 in TSLP gene were identified with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and genotypes and allele frequencies were calculated and statistically analyzed. Genotypes of Rs2289276 site in the asthmat...
Source: Comparative Clinical Pathology - December 16, 2017 Category: Pathology Source Type: research

Preoxygenation and injectable anesthesia in dog: evaluation of maintenance and recovery periods of anesthesia and hemoglobin desaturation
This study was conducted to evaluate the efficacy of preoxygenation during the injectable general anesthesia in dogs and estimate the maintenance and recovery times besides measuring the time of hemoglobin desaturation following preoxygenation. Ten young healthy mix-breed dogs were selected and divided into two equal groups. They premedicated with IM administration of acepromazine. The first group received pure oxygen via face mask for 5  min just before induction of anesthesia. The other group received room air similarly. The general anesthesia was induced with IV injection of ketamine-xylazine mixture. The SpO2 chan...
Source: Comparative Clinical Pathology - December 16, 2017 Category: Pathology Source Type: research

Molecular detection of Brucella spp in goat and sheep milk samples from apparently healthy and infected animals
This study set to assess the PCR assay for the molecular recognition ofBrucella DNA in goats and sheep unpasteurized milk samples in the southeast region of Iran and to determine the frequency distribution of seasonal outbreaks. Eight hundred samples of raw milk from 722 apparently healthy animals and 78 infected animals with a history of abortion were collected from January 2015 to June 2016. In our PCR, a specific genome region, the IS711 element, was targeted to amplifyBrucella DNA, and the frequency distribution of seasonal outbreaks was determined. PCR assay amplifiedBrucella DNA in 10.85% goats and 5.45% sheep milk s...
Source: Comparative Clinical Pathology - December 16, 2017 Category: Pathology Source Type: research

Pathological and histochemical studies of the effects of cystic echinococcosis in sheep
AbstractA total of 2100 sheep, slaughtered or spontaneously dead, from various areas of Kashmir valley were screened for the presence of hydatidosis. Out of 2100 cases, 85 were positive for hydatidosis. Histopathologicallly, the cyst wall consisted of the inner germinal, middle lamellated, and outer fibrous layer. Inflammatory reaction around the cyst was variable and was characterized by an inner zone of loosely arranged fibroblasts infiltrated with mononuclear cells and densely packed fibroblasts mixed with mononuclear cells; and an outer layer of concentrically packed fibrous connective tissue. Mast cell reaction was ob...
Source: Comparative Clinical Pathology - December 11, 2017 Category: Pathology Source Type: research

Evaluation of lipid-lowering effect of Cynara scolymus extract-loaded mesoporous silica nanoparticles on ultra-lipid-fed mice
AbstractIn current investigation, twenty mice were divided into 4 different equal groups as follows: group CS which received 0.1  mlCynara scolymus extract (diluted in distilled water) at dose of 600  mg kg−1 daily for 3  weeks, group A which received 0.1 ml Atorvastatin 40 (diluted in distilled water) at dose of 20 mg kg−1 daily for 21  days, group T which received 0.1 ml ofCynara scolymus extract at dose of 600  mg kg−1 daily in nonporous (diluted in distilled water) during experimental period, and control group (group C) which received the same vol...
Source: Comparative Clinical Pathology - December 8, 2017 Category: Pathology Source Type: research

Serum protein electrophoresis reference values in the gyrfalcon ( Falco rusticolus )
AbstractBlood samples were collected as part of routine clinical examination procedures from 120 clinically normal gyrfalcons (Falco rusticolus) during a 4-year period between the months of September and February. Serum protein electrophoresis (SPE) was carried out to establish reference values for the species and to characterize species-specific electrophoretic patterns. The SPE analyses included measurements of pre-albumin, albumin, alpha-1, alpha-2, beta-1, beta-2, gamma globulins, and the albumin/globulin ratio. Reference intervals were determined using a quantile approach with 90% confidence intervals. Female gyrfalco...
Source: Comparative Clinical Pathology - December 7, 2017 Category: Pathology Source Type: research

Seroprevalence of orf infection based on IgM antibody detection in sheep and goats from selected small ruminant farms in Malaysia
In conclusion, this study showed that a significant number of goat populations in Selangor, Malaysia, harbor active orf infection in comparison to sheep. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - December 7, 2017 Category: Pathology Source Type: research

Biochemical reference values for healthy captive Persian wild goat ( Capra aegagrus )
The objectives of this study were to establish reference values and to evaluate changes associated with gender and age for 22 mature, captured, clinically healthyCapra aegagrus. The reference values were obtained: glucose, 34.10 –204.90 mg/dl; urea, 11.81–49.45 mg/dl; creatinine, 0.075–2.032 mg/dl; triglyceride, 15.71–84.90 mg/dl; cholesterol, 10.02–131.30 mg/dl; total bilirubin, 0.011–0.875 mg/dl; direct bilirubin, 0.0–0.079 mg/dl; indirect bilirubin, 0.011–0.875 mg/dl; total protein, 3.294–7.460  g/dl; albumin, 2.315–...
Source: Comparative Clinical Pathology - December 3, 2017 Category: Pathology Source Type: research

Packed cell volume and bone marrow cytological responses in Trypanosoma vivax -induced acute trypanosomosis in Nigerian Sokoto Red goats
AbstractBone marrow responses have been identified as key determinants of trypanotolerance in cattle as they determine the ability for hemopoietic cell regeneration and control of anemia. However, not much is known about such responses in Nigerian breed of goats known to show different susceptibilities to trypanosome infections. Early bone marrow events associated with pathogenesis of Sokoto Red goats toTrypanosoma vivax were investigated in six goats to assess their role in susceptibility of this goat breed to trypanosomiasis. A total of six Sokoto Red goats of mixed sexes were used. While four of the goats randomly selec...
Source: Comparative Clinical Pathology - December 2, 2017 Category: Pathology Source Type: research

Clinical, morphologic, and demographic characteristics of bipartite patella in patients referred to Iranian academic clinics
AbstractIn a descriptive cross-sectional study, we evaluated clinical, morphologic, and demographic characteristics of bipartite patella (BP) in 20 patients referred to clinics affiliated to Iran University of Medical Sciences from 2011 to 2015. Simple radiography, computerized topography scanning, and magnetic resonance imaging characteristics of BP plus demographic data include age, gender, co-morbidities, and side of involvement were recorded. Male to female ratio was 17:3 (5.6) with total mean age of 39.1  ± 13.4 years. Sixteen patients (80%) had unilateral involvement, and among them right- a...
Source: Comparative Clinical Pathology - December 1, 2017 Category: Pathology Source Type: research

Clinicopathological study of tumour-like lesions of skin —a study of 238 cases
AbstractTumour-like lesions of the skin are more common than tumours of the skin and this study evaluates their pattern at a tertiary care centre in Maharashtra, India. This aim of this study was to analyse the various tumour-like lesions of the skin and to establish a clinicopathological correlation. All excision specimens received in the histopathology section of the department of pathology from January 2012 to August 2015 that were histopathologically proved to be tumour-like lesions of the skin were included in the study. The data was analysed followed by clinicopathological correlation. A total of 238 cases of tumour-...
Source: Comparative Clinical Pathology - November 28, 2017 Category: Pathology Source Type: research

Evaluation of combination therapy with hydrocortisone, vitamin C, and vitamin E in a rat model of intestine ischemia-reperfusion injury
This study was designed to use different antioxidant as hydrocortisone, vitamin C, vitamin E, and combination of them against experimental intestine ischemia-reperfusion (IR) for decrease free radical damage in soft tissue. Thirty adult male Wistar rats were selected randomly and divided into six equal groups. Group Sham: intact group, group control: IR group with 45  min of ischemia followed by 1 h of reperfusion, group vitamin C: IR plus vitamin C (50 mg/kg, IV) treatment immediately after reperfusion, group vitamin E: IR plus vitamin E (20 mg/kg, IM) treatment 15 min before reperfusion, group hy...
Source: Comparative Clinical Pathology - November 27, 2017 Category: Pathology Source Type: research

Serum biochemical values of some species of apparently healthy free-living wild birds in Zaria, Nigeria
In conclusion, there were interspecies variation s in biochemical parameters of free-living wild birds in Zaria, Nigeria. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 25, 2017 Category: Pathology Source Type: research

Toxicological evaluations of aqueous extracts of two Nigerian ethnobotanicals ( Tetrapleura tetraptera and Quassia undulata ) of neurological importance in rats
AbstractTetrapleura tetraptera (TT) andQuassia undulata (QU) are two predominant ethnobotanicals commonly used in traditional medical practices for the management of neurological disorders in Nigeria. However, there is a dearth of information on the toxicological aspects of these plants. Hence, in this study, a 14-day toxicological evaluation of aqueous extracts of TT and QU were carried out in rats. The extracts were administered orally with 5, 50, 500, 2000, and 5000  mg/kg body weight. Signs of toxicity were observed every hour for the first 6 h and every day for 14 days. Mortality, clinical signs, body w...
Source: Comparative Clinical Pathology - November 25, 2017 Category: Pathology Source Type: research

Comparison of feline serum amyloid A (SAA) measurements assessed by a point-of-care test analyzer to a validated immunoturbidimetric method
This study showed that the SOLO reliably and rapidly measures SAA in feline blood specimens; however, a “de novo” reference interval should be det ermined for proper interpretation of the results. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 25, 2017 Category: Pathology Source Type: research

Spontaneous white spot syndrome virus (WSSV) infection in mud crab ( Scylla serrata Forskal 1775) fattening pens farm of south east coast of India
AbstractThe white spot syndrome virus (WSSV) is one of the most serious diseases in the shrimp and crab farm. This virus disseminates very quickly and causes high mortality in culture pond, accompanied by great economic losses. Recently, high mortality of WSSV-infected mud crabs collected from M.G.R. Thittu, Cuddalore district, Tamil Nadu, south east coast of India during 2015 and 2016. A total of 283 samples collected from pens fattening ponds were tested for the presence of WSSV. Of these, a prevalence of 32.5% WSSV-infected mud crabs showed. Hence, the present study was carried to understand the reason for the mortality...
Source: Comparative Clinical Pathology - November 22, 2017 Category: Pathology Source Type: research

Determination of calcium, magnesium, phosphorus, iron, and copper contents in rooster seminal plasma and their effects on semen quality
AbstractIt has been documented that minerals affect male fertility and subsequent reproduction in human and animals. The aim of this investigation was to assess calcium (Ca), magnesium (Mg), phosphorus (P), iron (Fe), and copper (Cu) contents of rooster seminal plasma following normal feeding of diet and investigate their correlation with spermatozoa characteristics. Fifty-one semen samples were collected from ten roosters. Semen samples were evaluated concerning sperm motility and viability. Ca, P, Mg, Fe, and Cu contents of seminal plasma were evaluated by commercial kits. The mean concentrations of Ca, P, Mg, Fe, and Cu...
Source: Comparative Clinical Pathology - November 22, 2017 Category: Pathology Source Type: research

Povidone iodine versus silver nitrate in induction of sclerosing cholangitis in hepatic hydatid cysts surgery
AbstractThere are several scolicidal agents for inactivation of hydatid cyst protoscolices during surgery, but most of them are associated with adverse side effects such as sclerosing cholangitis and liver necrosis. The present study aims to evaluate the adverse effects of povidone iodine versus silver nitrate in producing sclerosing cholangitis in animal model. Twenty guinea pigs weighing 250 –350 g were used in this study. In one group, 0.3 ml 10% povidone iodine was used for 5 min in the biliary tree while in the second group, 0.3 ml 0.5% silver nitrate was used for the same amount of time. Aft...
Source: Comparative Clinical Pathology - November 21, 2017 Category: Pathology Source Type: research

Effect of zinc oxide nanoparticles and Bacillus coagulans as probiotic on growth, histomorphology of intestine, and immune parameters in broiler chickens
AbstractTwo hundred eighty-eight 1-day-old-male broilers were assigned to six dietary treatments to assess the influences of nanoparticles Zinc oxide (Zn-nan) and probiotic (Bacillus coagulans) supplements and their interaction on growing, histomorphology of intestine and immune parameters in chicks. Three amounts of dietary supplemental Zn source (100  mg/kg diet Zinc oxide, 25, and 50 mg/kg of diet Zn-nan) and two amounts of probiotic (0, 1010 CFU/kg of diet) were mixed as a randomized basic design with a 3 × 2 factorial experiment. The interaction effect of Zn source and probiotic wa...
Source: Comparative Clinical Pathology - November 20, 2017 Category: Pathology Source Type: research

Erythrocyte characteristics of the progenies of pure and reciprocal crosses of Pangasianodon hypophthalmus (Sauvage, 1878) and Clarias gariepinus (Burchell, 1822)
In this study, erythrocyte characteristic of pure and reciprocal crosses of African catfishClarias gariepinus (Burchell, 1822) and Asian catfishPangasianodon hypophthalmus (Sauvage, 1878) were evaluated. Blood smear was made from the progenies ofC. gariepinus (CG),P. hypophthalmus (PH), Pangapinus ( ♀PH × ♂CG) and in two morphotypes (Clarias-like and Panga-like) of Clariothalmus (♀CG × ♂PH) obtained from similar breeding history. Erythrocyte measurement of the cell and nuclear axis was done under the microscope, while many other parameters were calculated. Result obtained suggests a ...
Source: Comparative Clinical Pathology - November 20, 2017 Category: Pathology Source Type: research

Estimation of economic impacts due to hydatidosis among livestok  in Kermanshah Province, western Iran
AbstractInformation about inspection of slaughtered animals is a basic approach to review the epidemiological pattern of infectious diseases. Therefore, this study was aimed to determine the prevalence of hydatidosis as well as estimate of relevant economic losses in livestock slaughtered in Kermanshah Province, western Iran, throughout the years 2010 –2015. A total of 539,861 livestock examined; 12,413 (2.29%) were infected with hydatidosis. Overall, 4713 (3.21%) of cattle, 6720 (1.97%) of sheep, and 980 (1.86%) of goats were found to be infected with hydatidosis. The total economic loss was USD 457,582. The direct ...
Source: Comparative Clinical Pathology - November 19, 2017 Category: Pathology Source Type: research

Comparative effect of Melissa officinalis aqueous extract, sulfadimidine, and vitamin E –selenium on antioxidant parameters in rabbit experimental coccidiosis
This study was undertaken to assess the comparative effect ofMelissa officinalis aqueous extract, sulfadimidine, and vitamin E –selenium on some antioxidant parameters in experimental subclinical coccidiosis in rabbit. Forty adult Albino rabbits were alienated into five groups containing eight rabbits in each group. The control animals did not receive any infection or treatment. The remaining groups were inoculated with 1  × 104 infective oocysts ofEimeria perforans. Blood samples were collected before infection and 4, 8, 12, and 16  days post-infection (dpi) to measure antioxidant parameters...
Source: Comparative Clinical Pathology - November 18, 2017 Category: Pathology Source Type: research

The effect of the endotoxemia on the abomasal emptying rate in suckling lambs as shown by acetaminophen absorption test
This study was done on five suckling lambs in two phases. In phase 1 (control group), the first blood sample was obtained in 7  a.m. (time zero). Then a mixture of milk, barium sulfate 70%, and acetaminophen was administered orally to each lamb. Afterwards, blood samples were obtained each 15 min up to 4 h later. Phase 2 (treatment group) was similar to phase 1, but after the first blood sampling in time zero in 7 a.m., endotoxemia was induced by administration of commercial lipopolysaccharide at dose rate of 2 μg/kg. The rest of the processes of phase 2 were similar to phase 1. Cmax, Tmax, and ...
Source: Comparative Clinical Pathology - November 17, 2017 Category: Pathology Source Type: research

The first report of blood parasites in the birds in Fasa district, Southern Iran
AbstractTheHaemoproteus,Plasmodium,Aegyptianella, andLeucocytozoon parasites can lead to a reduction in the productivity and mortality of wild and domestic birds. The prevalence of these parasites was studied by random selection of 209 birds from different areas of Fasa district, Fars province, Southern Iran, during May to July 2015. The birds including the partridge, ostrich, quail, turkey, hen, rooster, and pigeon considered as native domestic birds and a group including of geese and ducks as migratory birds were studied. The overall prevalence of infection was 23.9%.Haemoproteus spp. (17.7%),Plasmodium spp. (1.5%), andA...
Source: Comparative Clinical Pathology - November 17, 2017 Category: Pathology Source Type: research

Tumor necrosis factor- α (TNF-α) −308 G/A and lymphotoxin-α (LT-α) +252 A/G genetic polymorphisms in Egyptian acute lymphoblastic leukemia
AbstractAcute lymphoblastic leukemia (ALL) is one of the most hematological malignancies of lymphoid origin. It has been proposed that deregulation of cytokines could be linked with pathogenesis, progression, and survival in many diseases. Genetic polymorphisms in two important cytokines like tumor necrosis factor- α (TNF-α) −308 and lymphotoxin-α (LT-α) +252 can disturb both their transcription and expression and lead to their high plasma levels. A difference in the occurrence of the polymorphisms in TNF-α −308 G/A and LT-α +252 A/G in ALL cases among several populations wit...
Source: Comparative Clinical Pathology - November 16, 2017 Category: Pathology Source Type: research

The effect of the anabolic steroid sustanon on the expression levels of neurotrophins in adult chicken gastrointestinal tract
AbstractNeurotrophins are well-known growth factors which can play integral roles in the control and regulation of the gastrointestinal tract (GIT). The anabolic steroid testosterone is involved in altering the contractile function of the stomach and intestine. The interactions between neurotrophin factors and testosterone are strong in many systems such as the nervous and respiratory systems. However, these interactions are not clearly determined in the gastrointestinal tract. The aim of this study is to investigate the effect of the anabolic steroid sustanon on the expression levels of neurotrophins in the chicken GIT. N...
Source: Comparative Clinical Pathology - November 15, 2017 Category: Pathology Source Type: research

Histological exhibition of the gastroprotective effect of Moringa oleifera leaf extract
AbstractThe gastroprotective activity ofMoringa oleifera leaf extract against aspirin-induced ulcers was investigated in rats. Thirty (30) rats under starvation but with access to drinking water for 48  h were divided into 6 groups of 5 animals each. Animals in groups 1 and 2 were pretreated with 0.2 ml normal saline via the oral route. Group 3 received 32 mg/kg cimetidine while those in groups 4, 5 and 6 received oral Moringa leaf extract treatments at doses 200, 400 and 800 mg/kg body weight respectively. Thirty minutes after treatment, all animals in groups 2 to 6 were given 800 mg/kg Aspirin to...
Source: Comparative Clinical Pathology - November 14, 2017 Category: Pathology Source Type: research

Virulent gene profile and antimicrobial susceptibility pattern of Shiga toxin-producing Escherichia coli (STEC) from humans in Maiduguri, Borno State, North-Eastern Nigeria
AbstractPrevalence and distribution of Shiga toxin-producingEscherichia coli (STEC) serogroups majorly linked with public health hazards in Maiduguri and the antimicrobial resistance pattern profile of the isolates were determined. Our results show that the prevalence of STEC isolated from human patients with gastroenteritis attending hospitals in Maiduguri was 1.2% of the 600 stool specimens examined. Two patients out of the seven STEC positive isolates had diarrhoea, and no significant statistical association (P 
Source: Comparative Clinical Pathology - November 14, 2017 Category: Pathology Source Type: research

Protective effect of garlic ( Allium sativum ) against zinc poisoning in the testicular tissue of goldfish ( Carassius auratus )
In this study, the protective effect of garlic (Allium sativum) against the toxic effect of zinc on the testicular tissues of goldfish (Carassius auratus) was assessed. Seventy-five healthy fish were allocated into 5 equal groups: control group, untreated; group 1, exposed to zinc sulfate (Merck, Germany) at dose of 3  mg/L; groups 2, 3, and 4 were also exposed to zinc sulfate in the same concentration as group 1 but their diet was supplemented with garlic (A.sativum) at 0.5, 1, and 2.5% of daily diet, respectively. After 45 days, 8 fish from each group were randomly selected, testicular tissue taken, and assessed his...
Source: Comparative Clinical Pathology - November 14, 2017 Category: Pathology Source Type: research

The coexistence of ovarian dysgerminoma and normal intra-uterine pregnancy presented with acute abdominal pain
We present here a rare and interesting case of dysgerminoma during pregnancy with literature review. A 29-year-old healthy pregnant woman presented with an acute progressive abdominal pain. The patient underwent laparotomy and left salpingo oophorectomy. A diagnosis of dysgerminoma (FIGO stage IA) was made. She had uneventful postoperative period. She referred for subsequent chemotherapy. The surgeons and radiologists, particularly at major referral emergency centers, are much more likely to be acquaint with ovarian dysgerminoma, as a solid nonhomogenous pelvic mass discovered by ultra-sonography in pregnant woman and its ...
Source: Comparative Clinical Pathology - November 13, 2017 Category: Pathology Source Type: research

BCR/ABL analysis in myelodysplastic syndromes
In this study, we dealt with evaluating the incidence of BCR/ABL fusion as a diagnostic and prognostic marker for MDS patients. Fifty patients were diagnosed as MDS by morphology and clinical examinations. Cytogenetic and molecular genetic analysis of BCR/ABL fusion was done on bone marrow (BM) biopsy and aspirate using fluorescence in situ hybridization (FISH) and nested-polymerase chain reaction (PCR) techniques, respectively. Descriptive data analysis was done using SPSS software, and Mann-Whitney and independent-samplet test were used to assess the correlation between variables. P210 BCR/ABL fusion was detected in two ...
Source: Comparative Clinical Pathology - November 12, 2017 Category: Pathology Source Type: research

Pure red cell aplasia in a Holstein calf
AbstractA 3-month-old Holstein calf presented with lethargy. Hematological examination showed severe non-regenerative anemia without reduction in white blood cell and platelet counts. Serum biochemical analysis revealed both high erythropoietin activity and high serum iron concentration. Bone marrow examination revealed selectively decreased erythroid precursors and an increased myeloid:erythroid ratio of 7:1. Lesions that could have caused anemia were found only in bone marrow by necropsy. The calf was diagnosed with pure red cell aplasia (PRCA) based on these clinical and pathological findings. Although the cause of PRCA...
Source: Comparative Clinical Pathology - November 12, 2017 Category: Pathology Source Type: research

Amblyomma ovale parasitizing a human
AbstractThe present study aims to report a case study regarding the parasitism in humans due toAmblyomma ovale in the west side of Santa Catarina, southern Brazil. The parasitized individual noticed the ectoparasite after visiting an area of forest and riverbank in that region, site also visited by cattle. The tick was then removed and placed in a 70% alcohol tube. In laboratory, it was visualized in stereomicroscope and identified according to the appropriate dichotomous key of the genus. The collected mite was an adult female, identified asA. ovale. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 10, 2017 Category: Pathology Source Type: research

Antidiabetic activity and free radicals modulatory potentials of saponin-rich extract of Cochlospermum planchonii (Hook Fx. Planch) root in vitro
This study probed the antidiabetic and free radical scavenging potentials of saponin extract fromCochlospermum planchonii root (CPS) in vitro. The antidiabetic activity was determined by the CPS inhibition of α-glucosidase and α-amylase activities while the free radical scavenging capacity was demonstrated against two, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radicals. The results illustrated that CPS robustly inhibited α-glucosidase and α-amylase (IC50 values  = 2.89 and 0.40 mg/mL), respectively as compared with acarbose. Both enzymes were uncompetitively inhibited as in...
Source: Comparative Clinical Pathology - November 9, 2017 Category: Pathology Source Type: research

In vitro analysis of antispasmodic activity of ethanolic stem extracts of Uvaria rufa Blume and Anomianthus dulcis (Dunal) J. Sinclair on excised rat ’s ileum
AbstractIrritable bowel syndrome (IBS) is a symptom of functional gastrointestinal disorders. It is commonly experienced as abdominal cramp, diarrhea, and constipation. The diagnosis and treatment of IBS were mostly conducted by synthetic medicines including antispasmodic drugs that have many side effects and are so expensive. The aim of the present study was to explore the antispasmodic activity ofUvaria rufa Blume andAnomianthus dulcis (Dunal) J. Sinclair extracts on isolated rats ’ ilia which later compared to atropine as reference drug. The ethanolic extracts from the stem ofA. dulcis andU. rufa were investigated...
Source: Comparative Clinical Pathology - November 9, 2017 Category: Pathology Source Type: research

Primary typical pulmonary carcinoid tumor: an incidental finding
We report a rare, asymptomatic case of a primary bronchopulmonary carcinoid in a 62-year-old man. A 62-year-old man presented with history of intermittent right upper quadrant pain, especially after a high fat meal, for 6  months. Systemic examination was unremarkable. Ultrasonography of gall bladder showed cholelithiasis, and he was scheduled for cholecystectomy. On preoperative cardiology consult, the chest X-ray showed an incidental homogenous opacity in the right middle zone without any respiratory symptoms. Pat ient underwent fiber optic bronchoscopy and biopsy. Histopathologic examination showed monomorphic tumo...
Source: Comparative Clinical Pathology - November 8, 2017 Category: Pathology Source Type: research

Ectoparasitism on freshwater fish in West Azerbaijan, northwest of Iran
AbstractEctoparasites are considered as one of the most important pathogens threatening fish health and production. Due to importance of freshwater fish as a main source of food and income and also little accessible information regarding the serious infectious agents, especially ectoparasites, in fish, the present study was carried out to determine and identify ectoparasite infestations in fish species in water resources of West Azerbaijan province, the northwest of Iran. Between June 2015 and January 2017, 280 freshwater fish belonging to families Cyprinidae and Salmonidae were collected and transferred to the laboratory ...
Source: Comparative Clinical Pathology - November 8, 2017 Category: Pathology Source Type: research

Biochemical profiles of hydatid cystic fluid from infected dromedary camels, cattle and goats in Oman
This study was performed to evaluate the electrolytes and biochemical profiles in hydatid cyst fluid (HCF) collected from 60 slaughtered animals (dromedary camels, goats and cattle) in Oman. The Beckman Coulter Chemistry Analyser was used to analyse the HCF aspirated from 30 fertile and 30 sterile cysts collected from livers and lungs. The results revealed that the means of sodium, calcium, glucose, magnesium, creatinine and total protein in dromedary camels, cattle and goats varied significantly (p 
Source: Comparative Clinical Pathology - November 8, 2017 Category: Pathology Source Type: research

Correction to: Preliminary use of Polygonum minus Linn. leaf extract on growth performance, feed utilization, and some hematological indices of Anabas testudineus (Bloch, 1792)
AbstractThe original version of this paper unfortunately captured the equation incorrectly and is corrected in this article. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 6, 2017 Category: Pathology Source Type: research

Generalized lymphoma with a displacing mass in the orbital cavity of a Pacific parrotlet ( Forpus coelestis )
AbstractIn this case report, a 4.5-year-old Pacific parrotlet (Forpus coelestis) with a generalized hematopoietic neoplasia is described. The patient was presented with severe unilateral exophthalmos of the right eye and a poor general health condition. Neither in the general nor the ophthalmologic, nor the subsequent microbiological and mycological examination signs of infection were detected, and molecular tests forChlamydia psittaci were negative. As the bird ’s condition decreased despite intensive care treatment, euthanasia was performed. Postmortem examination revealed a large, space-occupying, bloody mass in t...
Source: Comparative Clinical Pathology - October 12, 2017 Category: Pathology Source Type: research

Development of a rapid skin test for the diagnosis of brucellosis
This study has developed a rapid skin test for screening of humoral and allergic immune response toBrucella abortus. Two groups of mice were included in the research; a group immunized intraperitoneally withBrucella abortus bacterin twice at 2  weeks apart and a group kept as control which received PBS by the same route as case groups. Skin tests were performed 15 days after the second immunization byinjection of the detector solution (a composition of LPS-free sonicatedBrucella antigens, complement andpolyelectrolytes) into the abdominal skins of the sensitized animals.After that, the mice were checked for thege...
Source: Comparative Clinical Pathology - October 12, 2017 Category: Pathology Source Type: research