Mammary gland carcinoma “in situ” in a male dog: case report
AbstractMammary gland tumors are among the most frequently diagnosed neoplasms in female dogs. However, they are rare in male dogs, with incidence ranging from 0 to 2.7% (less than 1% on average). The triggering factors in males are still obscure, but studies have shown that hormonal abnormalities, especially those associated with testicular neoplasms, predispose towards their occurrence. Among the most common histological types diagnosed in males, adenocarcinoma, papillary cystadenocarcinoma, carcinosarcoma, simple adenoma, and spindle cell sarcoma can be highlighted. However, there are no reports of carcinoma “in s...
Source: Comparative Clinical Pathology - May 7, 2018 Category: Pathology Source Type: research

Baseline health indicators of Eastern Pacific Green Turtles ( Chelonia mydas ) from Baja California Sur, Mexico
The objective of this study was to establish a baseline of health indicators of free-ranging Eastern Pacific green turtles (Chelonia mydas) from neritic foraging and juvenile developmental habitats in Baja California Sur. Here, we contribute baseline and reference interval values of vital signs, complete blood count, and plasma blood chemistry for live specimens from coastal lagoons: Ojo de liebre and San Ignacio in Baja California Sur, Mexico. With the integration of these indicators, 79 turtles were diagnosed as “healthy.” Development of site-specific baseline of health indicators for free-ranging sea turtle ...
Source: Comparative Clinical Pathology - May 5, 2018 Category: Pathology Source Type: research

Effect of high dietary levels of α-tocopherol acetate on immune response of light and heavy weight male broiler breeders
In conclusion, dietary supplementation of α-TOH at higher levels (four times more than the strain nutrients recommendation) had beneficial effects on the immune response of both HW and LW male broiler breeders. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 5, 2018 Category: Pathology Source Type: research

Evaluation of the predictive value of Gensini score on determination of severity of coronary artery disease in cases with left bundle branch block
In this study, we aimed to examine the predictive role of Gensini score as marker of severity of coronary artery disease (CAD) in patients with LBBB and its relationship with other CAD risk factors and complications. We have included patients who referred to our center for selective coronary angiography with transient and or permanent LBBB on their surface electrocardiography (ECG). Cases with pacemaker rhythm and known non-CAD related causes of LBBB pattern on surface ECG were excluded from this study. Demographic variables and clinical features were studied. To determine severity of CAD, Gensini score was measured. The r...
Source: Comparative Clinical Pathology - May 5, 2018 Category: Pathology Source Type: research

Ivermectin resistance in intestinal parasites of camels in a private farm at Assiut, Egypt
AbstractRecently, anthelmintic resistance becomes a growing problem in veterinary medicine in all domestic animals including camels. There was a little documented research on parasitic resistance especially in camels in Egypt. Therefore, this study was designed to investigate resistance to ivermectin in one-humped camels (Camelus dromedarius) in a private farm at Assiut Governorate, Egypt. These camels were regularly receiving injectable ivermectin at 0.2  mg/kg bodyweight once every month and suffered from emaciation and loss of condition. Fecal samples were collected from all camels in the farm (number =&thin...
Source: Comparative Clinical Pathology - May 5, 2018 Category: Pathology Source Type: research

Occult hepatitis B infection: a hidden factor of poor response to intervention treatment of hepatocellular carcinoma in chronic hepatitis C patients
AbstractHepatocellular carcinoma (HCC), a tumor associated with high mortality, has rising worldwide incidence. Occult hepatitis B (OBI) could influence the development of HCC with a strong association with HCV infection. The risk of tumor recurrence or poor response to treatment may be due to the presence of underlying cirrhosis with viral hepatitis. The aim of this study is to assess the prevalence of OBI among patients who are hepatitis B core antibody positive with chronic hepatitis C (CHC) having HCC and assess its impact on treatment response. A total of 240 patients with CHC who were negative for hepatitis B surface...
Source: Comparative Clinical Pathology - May 4, 2018 Category: Pathology Source Type: research

A novel pathology of a mediastinal neoplasm
AbstractPrimary extragonadal malignant germ cell tumors (EMGCTs) are rare and account for only 2 –5% of malignant germ cell tumors. Vascular tumors are uncommon mediastinal masses making up
Source: Comparative Clinical Pathology - May 2, 2018 Category: Pathology Source Type: research

ABCB1 gene polymorphism in nephrotic syndrome
This study illustrates distribution of the most common three multidrug resistance gene 1 MDR1 (ABCB1) exonic polymorphismsG3435C,G2677  T/A, andC1236T in children with NS to assess their ability to be used as markers for response of NS to steroids. This study aimed to find association between ABCB1 gene polymorphisms and haplotypes and NS and if they can affect their response to steroids. ABCB1 gene polymorphisms in our cases were analyzed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Our cases were divided into three groups: one group included 50 steroid sensitive nephrotic synd...
Source: Comparative Clinical Pathology - May 2, 2018 Category: Pathology Source Type: research

Ameliorating effect of edible bird ’s nest against lead acetate toxicity on the rat hypothalamic–pituitary–ovarian axis and expressions of epidermal growth factor and vascular endothelial growth factor in ovaries
This study aimed to evaluate the protective effect of EBN supplement on the ovaries and pituitary glands of rats exposed to LA toxicity. Five treatment groups were established as follows: group 1 (C), which was given distilled water; group 2 (T 0), which was administered with LA (10 mg/kg body weight (BW); and groups 3 (T1), 4 (T2) and 5 (T3), which were given LA (10 mg/kg BW) plus graded concentrations of 30, 60 and 120 mg/kg BW of EBN, respectively. Rats were euthanised at day 35 to collect ovary and pituitary gland for histomorpholog ical study and the expression analysis of epidermal growth factor (EGF) ...
Source: Comparative Clinical Pathology - April 20, 2018 Category: Pathology Source Type: research

Gallotannin promotes cellular death through alteration of redox status
AbstractAvailable anti-cancer agents are beset with various challenges including limited efficacy, non-selectivity, and toxic side effect. These factors underpin the search for more effective alternative from plant materials or natural products. Meanwhile, plant polyphenols including gallotannin have been implicated for diverse medicinal potentials. In the present study, we investigated the toxic effect of gallotannin (GT) on human breast cancer cells (MDA-MB-231). GT reduced viability of breast cancer cells appreciably with an EC50 value of
Source: Comparative Clinical Pathology - April 19, 2018 Category: Pathology Source Type: research

Studying embryotoxicity of petroleum products for carp fish family
AbstractTo study the embryotoxicity of the oil fraction Karakuduk locality (Kazakhstan, oil deposit) for zebrafishDanio rerio (family Cyprinidae), in the conditions of the experiment. The method of breeding fishes ofDanio rerio, receiving, and incubating zebrafish in vitro. Experiments have been carried out on the development ofDanio rerio embryos in the oil-bearing fraction (Karakuduk locality) in various in vitro concentrations. The paper presents the results of experiments on exposure zebrafish embryos (Danio rerio) oil fractions in different concentrations and shows data on mortality of embryos and embryonic developmen...
Source: Comparative Clinical Pathology - April 17, 2018 Category: Pathology Source Type: research

Upregulation of two human microRNAs which might be involved in the carcinogenesis of nasopharyngeal cancer and that are promising for biomarker development
In this study, we analyzed the profile of miRNA expression in NPC biopsy tissue compared to normal tissues. The miRNA expression is taken from 246 samples of patients with NPC, compared to 17 sample s from non-NPC subjects as the control. The results of the analysis identified more than 100 miRNAs that underwent an upregulation of expression in NPCs compared to that in the control group. Further analysis was focused on understanding the role of the miRNAs that were upregulated in NPCs. The resu lts of this analysis reveal that there are six miRNAs: hsa-miR-1246, hsa-miR-320a, hsa-miR-1290, hsa-miR-146b-5p, hsa-miR-107, and...
Source: Comparative Clinical Pathology - April 14, 2018 Category: Pathology Source Type: research

Management of stage III benign mammary gland tumor in a 10-year-old female dog
AbstractA 10-year-old female German shepherd dog was presented with a large pendulous mass which was located in the area of the right inguinal mammary gland extending from M5 –M2 with no marked lymphadenomegaly. The mass has progressively increased in size without regression over a period of 1 year. Radiography revealed a radiodense mass of tissue with no apparent metastasis to regional lymph nodes, no pulmonary metastases, and no abdominal viscera metastases. The tum or was staged as T3N1M0 after diagnostic clinical and radiological examinations based on WHO standards for tumor stage. Unilateral radical mastect...
Source: Comparative Clinical Pathology - April 13, 2018 Category: Pathology Source Type: research

Effects of strain, sex and age on immunophenotyping parameters in the rat and mouse
AbstractImmunophenotyping of lymphocyte subsets can be a useful addition to toxicological or investigatory studies where there is the potential for effects on the immune system. We have validated assays for rat or mouse lymphocytes using a FACSVerse flow cytometer and FACSuite software, with commercially available antibodies. Whole blood was stained using the following markers: Rat —CD3, CD45RA, CD161a, CD4 and CD8; mouse—CD3e, CD19, NK1.1, CD4 and CD8. This allows detection and quantitation of T cells, B cells, NK cells, T helper cells and T cytotoxic cells. The stopping criteria for analysis were set at 30,00...
Source: Comparative Clinical Pathology - April 13, 2018 Category: Pathology Source Type: research

Hematological, biochemical, serological, and molecular monitoring of blood donor dogs vaccinated with CaniLeish ® for the prevention of Leishmaniosis
AbstractThe use of systemic preventive measures in dogs living in areas with Leishmania infection is advisable, particularly in blood donors. The aim of this study was to monitor healthy blood-donor dogs immunized with the vaccine CaniLeish ® by means of hematological, biochemical (serum total protein, albumin, protein electrophoresis, and C-reactive protein), serological (Speed Leish K™ and IFAT), and molecular assays (qualitative PCR, qlPCR). Twenty-four dogs were monitored at (T0), second (T1), third (T2) immunizations, and 2 mo nths (T3) after the initial immunization and at the first (T4), second (T5), ...
Source: Comparative Clinical Pathology - April 11, 2018 Category: Pathology Source Type: research

Association between hormonal and structural changes of the reproductive tract in estrus dairy cows
AbstractNormal cyclic changes in hormonal and ultrasonic echotexture along with estrus are critical for successful breeding in dairy cows. We studied relationships among steroid hormone concentrations and ultrasound changes of uterus and ovaries just before insemination in heat dairy cows. Eighty-nine estrus dairy cows were selected for transrectal examination of uterus and ovaries, and blood sampling just before AI. Ultrasound characteristics of the uterus and ovarian structures were recorded. The concentration of estrogen, progesterone, and cortisol was measured in serum of estrus cows. Our results showed that there was ...
Source: Comparative Clinical Pathology - April 11, 2018 Category: Pathology Source Type: research

Blood metabolite profile in Holstein-Friesian cows fed diets varying in dry matter and metabolizable energy density during early lactation
AbstractDietary effects on circulating concentrations of some blood metabolites were assessed in Holstein-Friesian cows in early lactation. Thirty-two lactating cows were randomly assigned to receive four diets differing in dry matter intake (DMI) and metabolizable energy (ME) density, namely low dry matter and metabolizable energy (LL), low dry matter and high metabolizable energy (LH), high dry matter and low metabolizable energy (HL), high dry matter and high metabolizable energy (HH), and concentrations of some blood metabolites determined. At the end of the 5-week study, the level of insulin-like growth factor-I (IGF-...
Source: Comparative Clinical Pathology - April 11, 2018 Category: Pathology Source Type: research

Phylogenetic analysis of Anaplasma marginale and Anaplasma ovis isolated from small ruminant based on MSP4 gene in western regions of Iran
AbstractAnaplasma is a genus of rickettsiales bacteria which is considered as a member of the tick-borne diseases. Different species of this genus are transmitted by blood-sucking insects and arthropods and blood-contaminated instruments. The present study was carried out to determine the prevalence ofAnaplasma ovis andAnaplasma marginale and to perform phylogenetic analysis in goat and sheep in East of Kermanshah provinces, western region of Iran. From April 2015 to May 2016, a total of 360 blood samples of small ruminants were collected. In the first step, DNA extraction and in the following nested PCR and the amplificat...
Source: Comparative Clinical Pathology - April 9, 2018 Category: Pathology Source Type: research

Methanolic extract of Celosia argentea var. crista leaves modulates glucose homeostasis and abates oxidative hepatic injury in diabetic rats
AbstractCelosia argentea commonly known as cockscomb plant is widely used in folkloric medicine in the treatment and management of diabetes mellitus. The effect of methanolic extract ofCelosia argentea var.cristata L. (CAVCL) leaves on blood glucose level, superoxide dismutase (SOD), catalase (CAT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities, and malondialdehyde (MDA) level were evaluated in diabetic rats. Five groups of male albino rats consisting of 5 animals each were used for the present study. They were grouped as normal control, diabetic control, diabetic a...
Source: Comparative Clinical Pathology - April 9, 2018 Category: Pathology Source Type: research

Comparison of anticancer effects of everolimus-gemcitabine combination with gemcitabine on pancreatic cancer using Rgs16 biomarker evaluation
This study showed that a combination of everolimus and gemcitabine is more potent than everolimus, which is more effective than gemcitabine, in killing pancreatic cancer cells. However,Rgs16 as a marker of invasion was very low in Panc-1 cells and the drugs were not effective in alteringRgs16 gene expression or protein level. Although this may suggest that Panc-1 is a very invasive cell line and these drugs are not effective in highly metastatic cases, the cell line might be resistant toRgs16 expression due to its specific mutations. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 8, 2018 Category: Pathology Source Type: research

Estimation of telomerase, AFP, and AFP-L3 levels in Sudanese patients with hepatocellular carcinoma and chronic liver diseases
AbstractTelomerase enzyme activity is considered a widespread and relatively selective tumor cell marker feature that can be used for detecting the presence of various neoplastic cells. The positive activity of telomerase enzyme was also recorded in chronic liver disease and is evaluated as a more appropriate biomarker for differentiating hepatocellular carcinoma (HCC) from chronic liver diseases. The current study aimed to evaluate telomerase, AFP, and AFP-L3 levels in Sudanese patients with hepatocellular carcinoma and chronic liver diseases. The study enrolled 113 patients: HCC (n = 33), cirrhosis (n =&...
Source: Comparative Clinical Pathology - April 6, 2018 Category: Pathology Source Type: research

Seroprevalence of brucellosis in different kinds of feline population in north-east of Iran
The objective of this survey was to assess the prevalence of brucellosis with focus onBrucella abortus andBrucella melletensis in different kinds of cats in north-east of Iran. Different kinds of cats included stray, pet, and cats which were located in dairy cattle herds tested. Blood sera of 48 (34.28%) stray, 42 (30%) pet, and 50 (35.71%) cats collected from 15 industrial dairy cattle herds. They were used for rapid and tube agglutination tests such as Rose Bengal, Wright, and 2-mercaptoethanol (2-ME). The comparison among age, sex, weight, and lifestyle of cats with serological tests of brucellosis were examined statist...
Source: Comparative Clinical Pathology - April 4, 2018 Category: Pathology Source Type: research

Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice
In conclusion, AM has nephroprotective property, thereby reducing the causation of diabetes in experimental mice. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 29, 2018 Category: Pathology Source Type: research

Cultural and immunohistochemical evaluation of bacterial agents in caprine pneumonia in Nigeria
This study evaluates the prevalence of important bacterial pathogens associated with caprine pneumonia in Nigeria using cultural and immunohistochemical techniques. One hundred and fifty goat lungs were randomly examined from macroscopic, cultural isolation and microscopic changes using standard techniques. The common bacteria identified were formalin inactivated for bacterine and polyclonal antibody production. Immunohistochemical staining was performed against the bacteria antigens using the avidin-biotin peroxidase complex technique. The data were presented in frequencies and percentages, and compared using non-parametr...
Source: Comparative Clinical Pathology - March 29, 2018 Category: Pathology Source Type: research

Retrospective evaluation of canine fractures in southern Nigeria
AbstractA retrospective analysis of clinical and radiographic records of dogs presented with bone fracture during the period from January 2006 to December 2016 was undertaken to analyze prevalence and causes of fractures in dogs presented to veterinary clinics in Delta State, in southern Nigeria. A total of 3212 case files were reviewed. 2.77% (n = 89) fracture cases affecting the pelvic and thoracic limbs were presented during the period under study. The incidence of fractures was highest in adult dogs (66.3%). The breeds mostly affected were mongrels (43.8%). Fractures were more in male dogs (71.9%) as comp...
Source: Comparative Clinical Pathology - March 28, 2018 Category: Pathology Source Type: research

Seroprevalence of peste des petits ruminants among unvaccinated small ruminants in Sokoto State, northwestern Nigeria
AbstractIn order to determine the current status of peste des petits ruminants (PPR) in Sokoto State, Nigeria, a competitive ELISA kit was used to detect the presence of antibodies to PPR virus among the unvaccinated sheep and goats from some randomly selected local government areas (LGAs) in the study area. An overall seroprevalence rate of 45.50% (197/433) was recorded for the disease in this study. Based on the LGAs sampled, the seroprevalence rates in decreasing order were 68.75% (Sokoto South), 59.10% (Tambuwal), 56.72% (Goronyo), 53.24% (Kware), 33.24% (Bodinga) and 18.60% (Wurno). Our results further revealed that s...
Source: Comparative Clinical Pathology - March 28, 2018 Category: Pathology Source Type: research

Bluetongue virus seropositivity and some risk factors affecting bluetongue virus infection in sheep flocks
AbstractBluetongue (BT) is an insect-transmitted, viral, and non-contagious disease in several species of domestic and wild ruminants. Sheep is the most susceptible host for bluetongue virus (BTV) in ruminants. In current study, 556 serum samples of ewes were assessed by competitive ELISA to investigate the serum status of BTV antibodies and risk factors affecting its seroprevalence in sheep flocks of Hamedan province, west part of Iran. In total, 256 out of 556 (46%) samples were found seropositive against BTV in all examined counties with different prevalence (ranging from 23.9 to 85%). The mean of age in positive and ne...
Source: Comparative Clinical Pathology - March 27, 2018 Category: Pathology Source Type: research

Effects of the hydroalcoholic extract of Artemisia khorassanica on wound healing potential in mice
In this study, the mean time to complete healing in groups control and placebo was 21  days, in group positive placebo 19 days, and in groups 10, 20 and 40% ointment 19, 18 and 16 days, respectively. All treatment groups were statistically significantly different when compared with the control groups (P 
Source: Comparative Clinical Pathology - March 26, 2018 Category: Pathology Source Type: research

Cytomorphometric analysis of exfoliated cells in patients with oral lichen planus
AbstractLichen planus is a mucocutaneous chronic inflammatory lesion with a potential for malignant transformation. Exfoliative cytology is a cheaper and less aggressive method for early diagnosis. The study was conducted to evaluate cytomorphometric changes of exfoliated cells of oral lichen planus lesions. This case-control study was accomplished on 33 patients with oral lichen planus and 50 patients who did not have any lesions as the control group. Buccal mucosa cells which were provided by five to ten reciprocating motion of hard toothbrush with constant tolerable pressure were spread on a slide; then, the samples wer...
Source: Comparative Clinical Pathology - March 25, 2018 Category: Pathology Source Type: research

Effects of bilateral whole vessel ovarian ligation on dogs ’ ovarian function and histopathology
In this study, all of the ovarian vessels were bilaterally ligated; then, ovaries were stimulated by gonadotropins. In a clinical trial and sequential control experiment, five healthy mixed-breed mature female dogs were randomly selected and kept under the new situation for 3  weeks. All ovarian vessels of the dogs were double ligated, bilaterally. For 13 weeks, blood samples, vaginal cytology, and ultrasound examinations continued weekly. All dogs received eCG (200 IU/dog, IM, 5 days) and hCG (500 IU/case IM, 5th day) 1 month after ovarian ligation. After 4 weeks , all dogs were ovariohy...
Source: Comparative Clinical Pathology - March 25, 2018 Category: Pathology Source Type: research

Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl 4 -induced nephrotoxicity in mice
In conclusion, GG has nephroprotective effect, thereby reducing the causation of toxicity in experimental mice. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 25, 2018 Category: Pathology Source Type: research

Comparative study of oxidative status in blood of asthmatic patients
AbstractBronchial asthma (BA) is a chronic inflammatory lungs disease, resulting in an airflow restriction, hyperactivity, and airway remodeling. The aim of present study is to investigate and compare the oxidative stress levels in blood of asthmatic patients differ in the disease control degree. In the current study were included 30 patients with bronchial asthma and 24 healthy volunteers. Patients were diagnosed with BA with allergic component longer than 1  year. For this purpose were explored the reactive oxygen species (ROS) and reactive nitrogen species (RNS), final products of lipids and proteins. Also, were st...
Source: Comparative Clinical Pathology - March 20, 2018 Category: Pathology Source Type: research

Physiological variations and the influence of age and sex on serum biochemical profile of hinnies
AbstractThere is little information about serum biochemistry in hinnies, animals that are usually confused with mules, so this is why specific diagnosis and treatment for these animals are difficult to obtain. Due to this fact, this study had the aim of offering elements to understand and determine references in serum biochemistry parameters specific for hinnies. To achieve this objective, biochemistry parameters from blood samples collected from 33 healthy animals (15 male hinnies with age between 7 and 84  months, and 18 female hinnies with age between 17 days and 84 months), from different farms in Brazil...
Source: Comparative Clinical Pathology - March 20, 2018 Category: Pathology Source Type: research

BIO (6-bromoindirubin-3 ′-oxime) GSK3 inhibitor induces dopaminergic differentiation of human immortalized RenVm cells
AbstractParkinson ’s disease (PD) is one of the most neurodegenerative disorders which can lead to severe neural disability and neurological defects. Cell-based therapy using fully differentiated cells is a new method for the treatment of this abnormal condition. In the present study, we investigated the effects of 6-bromoindirubin-3′-oxime (BIO) on dopaminergic differentiation of human immortalized RenVm cells in order to obtain a set of fully differentiated cells for transplantation in Parkinson’s disease. To this end, the immortalized RenVm cells were induced to dopaminergic differentiation using a neu...
Source: Comparative Clinical Pathology - March 19, 2018 Category: Pathology Source Type: research

Prognostic value of zymographic gelatinase activity of MMP-2 and MMP-9 in tumor recurrence of canine intrascrotal hemangiomas
This study was planned to identify the serum gelatinases in male dogs affected by intrascrotal hemangioma and determine their prognostic value in tumor recurrence. Ten dogs with intrascrotal hemangioma were diagnosed among a total of 65 testicular excision samples. According to the incidence of recurrence, the patients divided into two groups: tumors with recurrence and tumors with no-recurrence. Serum gelatinase activity was assayed by semi-quantitative zymography to determinate their prognostic value in canine intrascrotal hemangioma. Both latent and active MMP-9 and only latent MMP-2 appeared in the gels. Gelatinases sh...
Source: Comparative Clinical Pathology - March 19, 2018 Category: Pathology Source Type: research

Calcium metabolism-related hormonal changes in layers experimentally infected with Salmonella Gallinarum
In conclusion, the decrease in plasma calcium and phosphorus concentrations observed in theSalmonella Gallinarum experimentally infected layers was a consequence of hepatic, renal, and gastrointestinal lesions and not due to endocrine dysfunction as plasma levels of the hormones did not suggest so.Graphical abstractPhotomicrograph of the liver of layer experimentally infected withSalmonella enterica serovar Gallinarum. Areas of hepatic necrosis (black arrow). H&E. ×400. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 17, 2018 Category: Pathology Source Type: research

Sero-epidemiological study of peste des petits ruminants in small ruminants in Amahara region, Ethiopia
AbstractPeste des petits ruminants (PPR) is an acute, highly contagious, and economically important transboundary viral disease of sheep and goats. It is among the major infectious diseases that affect the production and productivity of small ruminants in Ethiopia. Cross-sectional and retrospective studies were undertaken to estimate the sero-prevalence, risk factors, and distribution of PPR in Amhara region. A total of 672 (329 sheep and 343 goats) sera were collected from 30 kebeles in 10 districts. Of the 672 sheep and goat sera samples tested using c-ELISA, 123 were positive for PPR antibodies, with the overall prevale...
Source: Comparative Clinical Pathology - March 16, 2018 Category: Pathology Source Type: research

Rodent-borne and rodent-related diseases in Iran
This study focuses on the distribution of rodent-related diseases in Iran. Rodent-borne and rodent-related diseases and diseases with suspected relationship with rodents have been reviewed in this study. Iran, due to the circumstances in which different species of rodents are able to live, has a high prevalence of certain diseases associated with rodents in urban and rural areas. Awareness about the distribution of rodent-related diseases can be a great help to rodent ’s control and prevention against the spread of the diseases. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 13, 2018 Category: Pathology Source Type: research

Glyphosate-based herbicide induces toxic effects on human adipose-derived mesenchymal stem cells grown in human plasma
AbstractGlyphosate (N-phosphonometil-glicine)-based formulations are herbicides widely used in world agriculture as well as in Brazilian territory. Therefore, it is essential to investigate the possible consequences of glyphosate exposure in human health. The effects of a glyphosate formulation (GLY-R) were assessed using human adipose-derived mesenchymal stem cells (hASCs), which possess multilineage differentiation potential, in an undifferentiated state and during differentiation of the cells. hASCs were exposed to a mixture containing basal or differentiation medium and GLY-R at a concentration of 36  μg m...
Source: Comparative Clinical Pathology - March 10, 2018 Category: Pathology Source Type: research

Investigation of NDM metallo-beta-lactamase and CMY-2 AmpC β-lactamase production in Escherichia coli and Enterobacter spp. isolated from human
AbstractNew Delhi metallo-beta-lactamase (NDM) and CMY-2-AmpC β-lactamase are among the most important resistance factors against carbapenem and beta-lactam antibiotics. Considering the few studies in Iran, here NDM and CMY-2 production inEscherichia coli (E.coli) andEnterobacter isolates were evaluated. Acquired resistance profiles of isolates were determined based on the new definitions as multi-drug resistant (MDR), extensively drug-resistant (XDR) and pan-drug resistant (PDR). In carbapenem-resistant strains, minimum inhibitory concentration (MIC) of different antibiotics by Etest method and the clonal relationshi...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Haematological changes in dogs with prolonged administration of high doses of sulphamethoxazole/trimethoprim
AbstractSulphamethoxazole/trimethoprim (SMX/T) is an antimicrobial agent that has been used for decades against both Gram-positive and Gram-negative bacteria as well as protozoa. It is particularly used as prophylaxis againstPneumocystis carinii pneumonia (PCP) in patients with acquired immune-deficiency syndrome (AIDS). Its use in dogs with associated adverse reactions has necessitated an experimental study that may enhance clinical management of the toxicities. The study aimed to determine the effects of prolonged treatment with high doses of sulphamethoxazole/trimethoprim on the haematological profile of dogs. Dogs were...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Human MTHFR -C677T gene transition increased risk of Alzheimer disease: a case-control study which performed by optimized PCR-CTPP method
AbstractMethylenetetrahydrofolate reductase (MTHFR) is a gene involved in folate metabolism which may contribute to the risk of Alzheimer disease (AD). In this paper, an association ofMTHFR-C677T single nucleotide polymorphism (SNP) with the AD was evaluated by optimized PCR-confronting two-pair primers (PCR-CTPP) genotyping method. For this aim at first, PCR-CTPP method was optimized for detection of this SNP by additive agents and touchdown PCR. Then, an association of the mentioned SNP with the AD was assessed in a case-control study comprising 113 Alzheimer ’s patients and 123 controls. Genotyping results showed ...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Investigation of NDM metallo-beta-lactamase and CMY-2 Amp. β-lactamase production in Escherichia coli and Enterobacter spp. isolated from human
AbstractNew Delhi metallo-beta-lactamase (NDM) and CMY-2-AmpC β-lactamase are among the most important resistance factors against carbapenem and beta-lactam antibiotics. Considering the few studies in Iran, here NDM and CMY-2 production inEscherichia coli (E.coli) andEnterobacter isolates were evaluated. Acquired resistance profiles of isolates were determined based on the new definitions as multi-drug resistant (MDR), extensively drug-resistant (XDR) and pan-drug resistant (PDR). In carbapenem-resistant strains, minimum inhibitory concentration (MIC) of different antibiotics by Etest method and the clonal relationshi...
Source: Comparative Clinical Pathology - March 8, 2018 Category: Pathology Source Type: research

Seasonal modulation of reproductive hormones and related biomarkers in coldwater cyprinid Barilius bendelisis (Hamilton, 1807)
AbstractBarilius bendelisis is a valuable ornamental and food fish from India in which the endocrine control of its reproduction activity has not been widely reported. The present study was aimed to elucidate changes in serum endocrine hormones: 17 β-estradiol (E2), testosterone (T), progesterone (P4), 17 α, 20β-dihydroxyprogesterone (17α, 20β-P), cortisol (C), triiodothyronine (T3), and thyroxine (T4); aromatase activity (ARO), vitellogenin (VTG), and total antioxidant capacity (TAC) during different seasons of the year 2014. All studied parameters showed marked seasonal variations. The highest ...
Source: Comparative Clinical Pathology - March 6, 2018 Category: Pathology Source Type: research

Endocrine disruption induced by bisphenol A in young and adult female Sprague Dawley rats
In conclusion, BPA exposure resulted in endocrine disruption indicated by different responses in hormonal and histopathological changes in both young and adult femal e rats. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 5, 2018 Category: Pathology Source Type: research

Acute toxicity and histopathological changes in livers of frog tadpoles ( Hoplobatrachus rugulosus ) exposed to bioinsecticides derived from Azadirachta indica A. Juss., Stemona curtisii Hook.F., and Mammea siamensis
AbstractAzadirachta indica A. Juss.,Stemona curtisii Hook.F., andMammea siamensis are popularly used as bioinsecticides by Thai farmers. To evaluate their safety on frog (Hoplobatrachus rugulosus) tadpoles, the ecologically and economically important species of Thailand, an acute static toxicity test was performed. The tadpoles (n = 10 per treatment group) were exposed to the bioinsecticides fromA. indica (NSAI 5, 10, 15, 20, and 25  g/l) and the mixture ofS. curtisii andM. siamensis (SCMS 0.8, 1.2, 1.6, 2.0, and 2.4  mg/l). It was found that the median lethal concentration (LC50) values at 96  ...
Source: Comparative Clinical Pathology - March 3, 2018 Category: Pathology Source Type: research

CD58; leucocyte function adhesion-3 (LFA-3) could be used as a differentiating marker between immune and non-immune thyroid disorders
AbstractThe link between Graves ’ disease (GD) and Hashimoto’s thyroiditis (HT) has been debated for decades due to the shared pathological and immunological components. Immune intolerance and inappropriate immune reaction against self-thyroid cells are distinctive features of both diseases, but definitive data for the clinical presentation of autoimmune thyroid disease remains unclear. To analyse the expression of T-regulatory cells, CD58, the CD4/CD8 ratio and the neutrophil/lymphocyte ratio and to determine if these parameters could be used as differentiating markers between auto- and non-immune thyroid dise...
Source: Comparative Clinical Pathology - March 3, 2018 Category: Pathology Source Type: research

Assessment of antioxidant and neuroprotective activities of methanol extract of Moringa oleifera Lam. leaves in subchronic chlorpyrifos-intoxicated rats
In conclusion, MO pretreatment reduced brain oxidative damages in rats sub-chronically-exposed to CPF. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 2, 2018 Category: Pathology Source Type: research

The impacts of yoghurt butter oil on rat testicular morphology and sexual hormones in a 150-day study
AbstractThe oil derived from traditional produced yoghurt has received much attention over the last two decades. While a high-fat diet (HFD) is assumed to be related to reproductive impairments, fermented dairy products are believed to have positive effects on the regulation of HFD-induced male reproductive damage. The reproductive effects of sheep ’s yoghurt butter oil (SYBO) have never been studied yet. The current study was to seek for the scientific evidence to determine SYBO effects on male rat reproductive system. In the present study, male Wistar rats were treated by standard diet or standard diet supplemented...
Source: Comparative Clinical Pathology - March 1, 2018 Category: Pathology Source Type: research

Evaluation of Houttuynia cordata Thunb. leaf extract on growth performance, feed utilization, and hematological indices of hybrid catfish ( Clarias macrocephalus × Clarias gariepinus )
AbstractThis research evaluates the effects ofHouttuynia cordata thunb. leaf extract on growth performance, feed utilization and the hematological indices of Hybrid catfish (Clarias macrocephalus×Clarias gariepinus). Four groups of fish were divided and given a basal diet (control group) as well as the extract solution, which was sprayed into the commercial fish feed (30% crude protein) in the amounts of the extract, 50, 70 and 100  ml/kg feed, then fed to the experimental fish for 90 days. Dominant results for the growth indices were found on days 15 and 30 of the experimental period. A significantly incre...
Source: Comparative Clinical Pathology - February 27, 2018 Category: Pathology Source Type: research