In vitro radical scavenging potential and toxicopathological implications of administration of aqueous leaf extract of Eucalyptus obliqua L.Her. (Myrtaceae) in Wistar rats
This study evaluated the in vitro antioxidant potential and toxicological effects of aqueous leaf extract ofEucalyptus obliqua via acute and 28-day once daily treatments in Wistar rats. In the acute toxicity test, rats were orally administered the extract up to 5000  mg/kg. The sub-chronic toxicity study was performed by once daily oral administration with the extract at 200, 400, 500, and 1000 mg/kg body weight for 28 days. The control rats received sterile distilled water. Biochemical and hematological indices were determined and a histopathological examin ation was carried out. The extract showed marked a...
Source: Comparative Clinical Pathology - July 17, 2017 Category: Pathology Source Type: research

FGFR3, a marker suggestive of favorable prognosis in urothelial carcinoma
In conclusion, high expression of FGFR3 in urothelial carcinomas with statistically significant relationships regarding its expression levels with parameters of favorable prognosis like low grade and pTa stage, presence of papillary architecture, and lower-stage urothelial carcinomas rai sed the probability that FGFR3 expression may have a role in early development of urothelial neoplasia. Further studies are required to investigate the applicability of FGFR3-targeted agents as an additional treatment regimen in patients with FGFR3 overexpression. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 9, 2017 Category: Pathology Source Type: research

Enterobius vermicularis infection among appendectomy specimens in Qom Province, Central Iran: a retrospective study
AbstractThe role of parasites in the pathogenesis of appendicitis has long been discussed.Enterobius vermicularis (E. vermicularis) is the most common parasitic worm found in the removed appendices. The main objective of this study was to determine the prevalence of parasitic infections among appendectomy specimens in Qom Province, Central Iran. This retrospective study was conducted at Nekoii Hospital of Qom City, from 21 March 2005 to 19 March 2016 (11-year period). The histopathology reports and recorded demographic data concerning 13,744 performed appendectomies were reviewed and then extracted using a structured templ...
Source: Comparative Clinical Pathology - July 9, 2017 Category: Pathology Source Type: research

Blue-black grassquit ( Volatina jacarina ) leucocyte and average weight reference values
AbstractThe bird speciesVolatina jacarina is popularly known as blue-black grassquit. They are widely distributed geographically and are not considered threatened for extinction. They are popularly domesticated because of their pleasant singing they can be found in many households. That means Veterinarians are sometimes confronted with those animals. This requires a certain knowledge about these birds, including in terms of reference values. Due to the lack of hematological data for this species, the present study aimed to calculate reference values for leukocytes and mean weight for this bird. To this end, 13 blue-black g...
Source: Comparative Clinical Pathology - July 9, 2017 Category: Pathology Source Type: research

Ultrastructure of bioscaffolds derived from bovine articular cartilage as an experimental model to support blastema cells
AbstractDecellularized cartilage extracellular matrix (ECM)-derived bioscaffolds have been designed as they provide a unique opportunity to carry out research on the mechanisms of chondrogenesis and its regulation. These scaffolds are used as a model in order to emulate different aspects relating to the formation, degeneration, and regeneration of the cartilage. This project has studied the interaction between the decellularized bovine articular cartilage and the blastema cells derived from the punched rabbit ’s ear. Articular cartilage was dissected in fragments with 10 mm length and 2 mm thickness. Then, ...
Source: Comparative Clinical Pathology - June 24, 2017 Category: Pathology Source Type: research

Report and Abstracts for 17 th Spring ACCP Conference with Training Workshops, and Technology Exhibits
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 20, 2017 Category: Pathology Source Type: research

Effects of age on the reproductive performance of different males and females in bighead carp Hypophthalmichthys nobilis (Richardson, 1845)
AbstractThe age of fish can affect reproductive performance in relation to the gamete quality and productivity which might be expected to be changed in older individuals. The consideration can be important in artificial propagation in relation to the selection of broodstock. We determined the age-related changes in male and female reproductive characteristics of the bighead carpHypophthalmichthys nobilis and then we examined the effects of age on the male and female reproductive fitness in terms of fertilization, hatching, and larvae survival rate. A total of eight 3 –4-year-old males and eight 4–5-year-old fem...
Source: Comparative Clinical Pathology - June 19, 2017 Category: Pathology Source Type: research

Vitrification of bovine ovarian tissue: effect of perforated antral follicles on the structural preservation of follicles
The objective was to evaluate whether follicular perforation could improve the survival of antral follicles after vitrification. The present study was conducted on 20 bovine ovaries collected at slaughterhouse and a total of 344 antral follicles (less than 4  mm in diameter). For each ovary, three slices (approximately 1 × 1 cm) were separated from the cortex of each ovary and randomly were assigned in three groups, control (without freezing, fixed in 10% buffered formalin), non­perforated vitrified and perforated vitrified (after exposure to equi libration solution, antral follicles were imme...
Source: Comparative Clinical Pathology - June 17, 2017 Category: Pathology Source Type: research

Comparative and analytical study on active toxoplasmosis to assess the IgG avidity in correlation to serological profile in a cohort of Egyptian patients
AbstractProper diagnosis of toxoplasmosis is very important for proper treatment in manifested cases and to prevent congenital toxoplasmosis in case of pregnancy. Screening of 180 pregnant females by automated immune assay forToxoplasma IgG and IgM Abs was done first then positive cases and some of negative cases were evaluated for theToxoplasma IgG avidity test versusToxoplasma IgM and IgA Abs using ELISA technique for the detection of active toxoplasmosis during gestation using IHA rising titer as reference test. Assessment ofToxoplasma IgM by ELISA compared with rising titer of IHA as a reference test revealed that the ...
Source: Comparative Clinical Pathology - June 17, 2017 Category: Pathology Source Type: research

Are surface markers of cancer stem cells reliable factors for cancer treatment follow-up?
AbstractRecent studies have explained that cancer stem cells (CSCs) can both instigate and maintain tumours. As a result, they are a good choice for targeted therapy in the treatment of aggressive cancers, and their long-term assessment is essential for the follow-up of therapeutic agent efficacy. However, several studies have revealed that their detection on the basis of surface markers is challenging. In this area, there are two main questions. First, why are surface markers of the CSCs so difficult to detect in in vitro studies? Second, are CSCs ’ surface markers reliable factors when assessing cancer treatment ef...
Source: Comparative Clinical Pathology - June 16, 2017 Category: Pathology Source Type: research

Level of cystatin C in functional thyroid disorders and its relation to GFR
AbstractThyroid hormones affect the development and physiology of the kidneys. Hypothyroidism and hyperthyroidism affect glomerular filtration rate (GFR), tubular function, renal blood flow, and structure of the kidney. Cystatin C is a non-glycosylated neuroendocrine protein. It is freely filtered at the glomerulus and completely reabsorbed and catabolized by cells of tubules. Cystatin C levels, affected by the thyroid state, is being increased in hyperthyroidism and decreased in hypothyroidism. The current study was conducted on 66 patients of thyroid disorder and 21 healthy control subjects. Total T3, total T4, and TSH w...
Source: Comparative Clinical Pathology - June 6, 2017 Category: Pathology Source Type: research

Evaluation of haematological and serum biochemical changes associated with constant rate infusion tramadol hydrochloride as an adjunct to ketoprofen in laparotomized and ovariohysterectomized dogs
This study evaluated changes in haematology and serum biochemistry associated with constant rate infusion (CRI) tramadol hydrochloride as an adjunct to ketoprofen for analgesia in adult female dogs following laparotomy (LP) and ovariohysterectomy (OVH). Thirty (30) adult female dogs randomly assigned into six groups of five dogs each were used in studies 1 and 2. In both studies, group 3 served as the control which received normal saline while groups 1 and 2 were given 1.0 and 2.0  mg/kg/h of tramadol Hcl, respectively. Dogs in study 1 were laparotomized while OVH was performed on dogs in study 2. Ketoprofen (4 m...
Source: Comparative Clinical Pathology - June 3, 2017 Category: Pathology Source Type: research

Association between beta globin haplotypes, HBBP1 and HMOX1 polymorphisms in relation to HbF among sickle cell anemia patients: a study in Southwest Iran
In this study, rs2071348 and rs2071746 polymorphisms in HBBP1 and heme oxygenase 1 (HMOX1) genes associated with different haplotypes and HbF levels in patients with sickle cell anemia were evaluated in comparison with healthy subjects. In this case-control study, 150 patients with sickle cell anemi a (90 homozygous and 60 sickle-cell trait) were evaluated and compared with 50 healthy individuals referred to Shafa tertiary hospital in Ahvaz city of Khuzestan Province, Iran. HbF level was measured by alkali and acid electrophoresis. PCR-RFLP and ARMS-PCR methods were used to determine β-globin gene haplotypes and HBBP1...
Source: Comparative Clinical Pathology - May 31, 2017 Category: Pathology Source Type: research

Effects of Nigerian red propolis in rats infected with Trypanosoma brucei brucei
AbstractPropolis has a long history of use in folk medicine and possesses various biological activities. Effects of the methanol extract of Nigerian red propolis (NRP) in trypanosome-infected rats were studied. Mature albino Wistar rats (30) were randomly grouped into six (A –F). Groups A–E were infected with 1.0 × 106Trypanosoma brucei brucei organisms intraperitoneally. Group F was uninfected. On establishment of infection, groups A –C were treated orally with NRP at 600, 400 and 200 mg/kg body weight for five consecutive days while group D received diminazene aceturate at 7 mg...
Source: Comparative Clinical Pathology - May 31, 2017 Category: Pathology Source Type: research

Modulation of immunity and inflammatory gene expression in buffalo ( Bubalus bubalis ) with some digestive disorders
AbstractSo far, there has been scarce information about the status of immunoglobulins (Ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. The purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. For this reason, 50 native breed water buffaloes were studied. Forty of these buffaloes showed various symptoms of digestive disorders and were allocated into 4 groups of equal sizes (group 1: uncategorized stomatitis; group 2: acute traumatic reticuloperitonitis [TRR]; gro...
Source: Comparative Clinical Pathology - May 31, 2017 Category: Pathology Source Type: research

Inhibitory effects of lactic acid bacteria isolated from traditional fermented foods against aflatoxigenic Aspergillus spp.
AbstractIn the present study, after molecular identification of dominant lactic acid bacteria (LAB) isolated from traditional fermented foods, antifungal activity of the isolates was investigated against aflatoxigenicAspergillus spp. Based on screening results, among the isolated LAB,Pediococus lolii had the highest inhibitory effect againstAspergillus flavus (70.32%) andAspergillus niger (98.8%). Furthermore, antifungal activity ofP. lolii stationary phase cell-free supernatant (CFS) was significantly (P 
Source: Comparative Clinical Pathology - May 27, 2017 Category: Pathology Source Type: research

Biochemical evaluation of amniotic and allantoic fluids of the Red Sokoto goat in the second term of pregnancy —a preliminary study
This study evaluated the biochemical profile of the allantoic and amniotic fluids of pregnant Red Sokoto goats in their second term of pregnancy. Aliquots of each of the allantoic and amniotic fluids were aspirated and placed into separate clean sample bottles. These foetal membrane fluids samples were preserved at −20° and used for the biochemical evaluations. The foetuses were exteriorized, and the sexes of the foetuses were noted, while their crown-rump lengths (CRL) were measured. The CRL was used for gestational age determination. Allantoic and amniotic fluids from does of gestational age between 50 an d 100...
Source: Comparative Clinical Pathology - May 27, 2017 Category: Pathology Source Type: research

Protective effects of royal jelly on the histomorphologic, oxidative stress and sperm parameters in Ofloxacin treated rat
AbstractRoyal jelly is produced by worker bees as nutrition for bee larvae and adult queens and has also been shown to have protective effects against antibiotics. The aim of this investigation was to determine protective effect of royal jelly on the reproductive functions of male rats treated with ofloxacin. In this experiment, 32 mature male albino rats were randomly allocated into four groups (n = 8): control, ofloxacin only, royal jelly only, and ofloxacin with royal jelly. The results revealed that ofloxacin alone caused significant decreases (P 
Source: Comparative Clinical Pathology - May 27, 2017 Category: Pathology Source Type: research

The central effect of 3-iodothyronamine on brain neuropeptides in mice
In this study, we attempted to find out whether chronic low dose 3-iodothyronamine treatment could modulate some food intake regulatory neuropeptides such as leptin, ghrelin, and galanin in mice brain. Eighteen male mice were divided randomly into control (n = 8) and treatment (n = 10) groups. The experimental procedure was applied for 7 days during which treatment group received T1AM (i.p) whereas the control group received DMSO and normal saline. The brain was analyzed for leptin, ghrelin, and galanin concentrations. There were significant differences in leptin concent ration (1.75 ±&...
Source: Comparative Clinical Pathology - May 24, 2017 Category: Pathology Source Type: research

Ultrasonographic and histopathologic changes following injection of neutral zinc gluconate in dog ’s ovaries
In this study, four female healthy and fertile dogs were selected randomly. Under general anesthesia and during laparotomy, the neutral sterile zinc gluconate was injected directly into the ovaries of five dogs. Changes in the size and appearance of the ovaries, leukocytes , and vaginal cytology were monitored before and after the start of the study twice a week. After 1 month, the uterus and ovaries were removed surgically and sent to laboratory for histopathologic examination. Except one dog which was in diestrus, the other dogs were in anestrus during the study. B ased on ultrasonographic examination, zinc gluconat...
Source: Comparative Clinical Pathology - May 22, 2017 Category: Pathology Source Type: research

Emphysematous lymphangitis in the colon of a steer
AbstractEmphysematous lymphangitis was found in the colon of a 2-year-old Holstein-Friesian steer which was conventionally slaughtered for food. At postmortem examination, the wall of a circumscribed region of the colon was involved with emphysematous lesion characterized by the presence of many small gas bubbles. Histologically, this emphysematous lesion proved to be multicystic lymphangiectasis, mainly involving the submucosal tissue. Lymphangiectasis was associated occasionally with granulomatous infiltrates comprised of giant cells and macrophages. A small number of macrophages and eosinophils infiltrated into areas su...
Source: Comparative Clinical Pathology - May 22, 2017 Category: Pathology Source Type: research

Comparing the Vitex-castus extract and cabergoline effects on estrous induction in female dogs
AbstractCabergoline is used to induce estrus, stop lactation, and induce abortion in small animals. The specific doses of Vitagnus extract have almost identical properties of cabergoline that reduce prolactin and increase the concentration of FSH hormone. The aim of this study was to compare the effect ofVitex agnus-castus extract and cabergoline on prolactin concentration and hormonal changes associated with the onset of estrous cycle in dogs. Twelve healthy adult cyclic female-mix breed dogs were selected and randomly aligned in treatment (6 dogs, Vitagnus extract 1.5  ml daily for 5 weeks, 80–90 mg/...
Source: Comparative Clinical Pathology - May 21, 2017 Category: Pathology Source Type: research

Reference values for some clinical, hematobiochemical and electrocardiographic parameters in miniature donkeys: the effect of sex, age and season
AbstractIn the current study, we aimed to establish reference values for the most important clinical, hematobiochemical and electrocardiographic (ECG) parameters and evaluate the effect of sex, age and season in miniature donkey. We hypothesized that these parameters in this valuable breed of donkey would be different from those of other species of equidae. Twenty apparently healthy miniature donkeys aged 1 –8 years and weighing averagely 115 ± 10 kg, with an average height of 0.83 ± 0.3 m, were used in this study. Based on sex, age and season of sampling, the animals ...
Source: Comparative Clinical Pathology - May 19, 2017 Category: Pathology Source Type: research

Monepantel in the control of Haemonchus spp. and Trichostrongylus spp. and possible side effects of treatment in naturally infected sheep
AbstractSheep breeding represents an important economic source in many countries; however, anthelmintic resistance is an important limiting factor for sheep breeding development, due to its effects in animal performance. Monepantel is an active principle derived from an amino-acetonitrile class, with proven efficacy in the control of gastrointestinal parasites. However, the side effects associated to this treatment remain unknown. Thus, the aim of this study was to evaluate the effect of anthelmintic treatment with monepantel as well as evaluate the hepatic function, proteic and lipid metabolism, and antioxidant/oxidant le...
Source: Comparative Clinical Pathology - May 17, 2017 Category: Pathology Source Type: research

Topical co-administration of Pistacia atlantica hull and Quercus infectoria gall hydroethanolic extract improves wound-healing process
AbstractPistacia atlantica hulls andQuercus infectoria galls have been used in traditional medicine for treatment of many disorders. In the current study, excision wound model was used for the assessment of wound-healing activity of topical co-administration of hydroethanolic extracts ofPistacia atlantica hulls (P. atlantica) andQuercus infectoria galls (Q. infectoria) in streptozotocin-induced diabetic mice. Four groups of diabetic mouse model were used; control group (received soft yellow paraffin), treated groupsP. atlantica 5%;Q. infectoria 5% andQ. infectoria 5%  + P. atlantica 5% mixed soft yellow paraffin....
Source: Comparative Clinical Pathology - May 13, 2017 Category: Pathology Source Type: research

Isolation and identification Candida vulvovaginitis in women referred to health centers of Arak City and antifungal activity of Equisetum arvense and Quercus on Candida albicans
This study investigated 152 patients suspicious of vulvovaginal candidiasis. Seventy-five isolates were identified from 152 isolates ofCandida albicans, and 55 yeast isolates were identified as non-albicans (Candida krusei,Candida glabrata, andCandida tropicalis), and 22 yeast isolates were unidentified. In broth microdilution method,MIC50 was reported as 12.5  μl/ml inCandida albicans forEquisetum arvense andQuercus, and MFC was reported as 25  μl/ml for both plants. In this research, isolation ofCandida albicans species is more than that of non-albicans species. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 13, 2017 Category: Pathology Source Type: research

Impact of iron deficiency anemia on CD4 and CD8-T lymphocytes among preschool-school children
AbstractIron is a basic element at cellular and molecular levels for proper development and effective function of the immune system. Children with iron deficiency are more susceptible to infections and have many alterations to their immune profile. The current study investigates the quantitative changes for CD4 and CD8 T-lymphocytes among symptomatic and asymptomatic iron deficient children. Flow cytometry using conjugated monoclonal antibodies was used for assessment of the CD4 and CD8 T-lymphocyte subsets among 30 asymptomatic iron deficient children, 28 iron deficiency anemia (IDA) symptomatic children, and 30 healthy c...
Source: Comparative Clinical Pathology - May 11, 2017 Category: Pathology Source Type: research

Effects of Salvia officinalis L. on 7, 12-dimethylbenz[a]anthracene-induced breast cancer in rats and mouse mammary carcinoma cells (4T1)
AbstractThe toxicity ofSalvia officinalis L. on different cancer cell lines has been reported extensively, but an in vivo evaluation on breast cancer has not been conducted. Therefore, in vivo and in vitro studies of are evaluated in this study. The hydro-alcoholic extract ofSalvia officinalis L. was studied in female Wistar rats in three groups: one control (Con) group (n = 7) and two 7, 12-dimethylbenz[a]anthracene cancer-induced groups (CI) that were gavaged either with distilled water (Con-CI) or 30 mg/kg sage hydro-alcoholic extract (SHE-CI) for 30 days (n = 34). A mammary gland whole mou...
Source: Comparative Clinical Pathology - May 10, 2017 Category: Pathology Source Type: research

Hematological and serum biochemical alterations in buffalo with some digestive disorders
AbstractHematological and serum biochemical reference intervals are essential laboratory variables for optimal diagnostic purposes. The present study was delineated to provide an insight into the hemato-biochemical alterations as well as the measurement of selected acute phase proteins in buffaloes showing some digestive disorders. The present study included 40 native breed water buffalo showing various symptoms of digestive troubles. Based on competent case history, clinical and laboratory findings, the diseased buffaloes were allocated into four equal sized groups, namely stomatitis, acute traumatic reticuloperitonitis (...
Source: Comparative Clinical Pathology - May 10, 2017 Category: Pathology Source Type: research

Cryptosporidium genotypes and associated risk factors in a cohort of Egyptian children
AbstractCryptosporidium is one of the most common, worldwide diarrheal diseases caused by parasites. Due to absence of an effective treatment, determining the prevailing species ofCryptosporidium is a key in identifying its transmission dynamics and a necessary precursor required for the planning and implementation of effective preventive and control strategies. This PCR-RFLP study was done to determine the prevalence ofCryptosporidium species in the stool of a cohort of Egyptian children and evaluate/assess associated risk factors for susceptibility to cryptosporidiosis, due to the lack of existent studies addressingCrypt...
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

Generalized lower motor neuron paralysis associated with Toxocara canis in a husky puppy
AbstractToxocara canis is a parasitic, ascarid nematode considered to be one of the most prevalent gastrointestinal helminths of domestic dogs and other canids worldwide. The larvae of this parasite in dogs migrate to the lungs and liver to complete the life cycle, thus respiratory symptoms such as cough and nasal discharge and vomiting may be observed. Neurological symptoms in human beings have been reported due to migration of the larvae of the parasite to the central nervous system. Beside, neurotoxocariasis associated with lower motor neuron paralysis has been reported in a male individual but such neurological symptom...
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

Prevalence of Sarcocystis infection in processed meat products by using digestion and impression smear methods in Hamedan, Iran
This study aimed to detectSarcocystis parasite in processed meat products in Hamedan, Iran. A total of 20 samples of hamburger, sausage, and cocktail were collected from markets from three factories in Hamedan, Iran. The samples were examined by digestion and impression smear methods for detectingSarcocystis parasite. The results showed that 80% of all tested samples were infected withSarcocystis. The infection rate in hamburger, sausage, and cocktail were 87.5, 83.33, and 66.66%, respectively. The highest infestation rate was observed in hamburger. The present study shows that the rate ofSarcocystis contamination in meat ...
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

Preliminary report on the age-related variations in the serum and seminal plasma testosterone, seminal plasma biochemistry and seminal vesicle histochemistry of male broodstock African catfish ( Clarias gariepinus )
This study investigated the age-related changes in the concentrations of serum testosterone and seminal plasma testosterone, ions, total protein, glucose concentrations and alkaline phosphatase (AP), amino aspartate transferase (AST) activities and the periodic acid Schiff-Alcian blue histochemistry of the seminal vesicle of cultured male broodstock African catfish of 6, 9 and 12  months of age. Each of these months constituted an age group and had five fish. The mean seminal plasma testosterone concentrations of the 6 months-age group were significantly higher (P 
Source: Comparative Clinical Pathology - May 9, 2017 Category: Pathology Source Type: research

TRPV1 gene polymorphisms in patients with diabetes compared with healthy individuals
In this study, no significant association was found betweenTRPV1 gene polymorphisms and the presence of type 1 diabetes. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 8, 2017 Category: Pathology Source Type: research

The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats
AbstractDiabetic retinopathy is a metabolic disease and oxidative stress-associated diabetic disorder. The antioxidant plants are important in the treatment of diabetic mellitus. The main purpose of this study was to determine the effect ofAmygdalus scoparia on anti-diabetic and antioxidant activity in the photoreceptor layer of diabetic retina. Thirty rats were housed and divided into three groups of 10 rats each. Group I: control group; group II: Diabetic group (induced by IP injection of 50  mg/kg of stereptozotocin); group III: diabetic receiving 200 mg/kgA. scoparia extract 1  month before and 2 mo...
Source: Comparative Clinical Pathology - May 8, 2017 Category: Pathology Source Type: research

Association between oestrogen receptor β immunoexpression and cause of culling, ovarian appearance and the existence of PRRS virus in the porcine ovary
In conclusion, the gilts culled due to anoestrus had relatively poor ovarian ERβ immunoexpression. Neither the existence of PRRS virus nor ovarian appearance was associated with ERβ immunoexpression in the gilt ovary. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 5, 2017 Category: Pathology Source Type: research

Cure rate of postpartum endometritis after different treatments in high produce dairy cows
AbstractThe main objective of this study was to compare the common treatment methods of clinical endometritis (CE) including injection of PGF2a and intrauterine infusion of cephapirin benzathine (CB) with intrauterine infusion of 50% dextrose solution (DEX) and intrauterine infusion of liquid paraffin (LP) in dairy cows. To this end, 188 lactating Holstein cows with clinical signs of endometritis were randomly assigned into five treatment groups and cytological smear was prepared. In group 1 (n = 37), cows were treated with intrauterine infusion of CB. In the second group (n = 53), the treatment protoco...
Source: Comparative Clinical Pathology - May 4, 2017 Category: Pathology Source Type: research

Respiratory tract cadmium-induced injuries —poisoning via intake and water pH could influence their genesis? An experimental study in rats
AbstractCadmium exposure may result in a variety of pulmonary diseases. No studies have evaluated tracheal damage or even whether cadmium intake can damage the respiratory tract. We evaluated the possible injuries caused by cadmium poisoning via intake into the respiratory tract and the possible effects of water pH in their genesis. Ninety male Wistar rats were divided into six groups (n = 15): GC5—received CdCl2 (400 mg/l) in the drinking water at an acidic pH of 5; GC7 received CdCl2 (400 mg/l) in the drinking water at an acidic pH of 7.0; GC8—received CdCl2 (400 mg/l) in the drinking...
Source: Comparative Clinical Pathology - May 3, 2017 Category: Pathology Source Type: research

Primary form of malignant melanoma in an ovarian mature cystic teratoma: case report and literature review
AbstractGerm cell tumours are a common form of ovarian neoplasm; the most common of which is mature cystic teratoma. However, transformation into malignant melanoma is rare. Here, we present a case of a primary malignant melanoma in an ovarian mature cystic teratoma with literature review. A 63-year-old woman presented at our hospital with abdominal distension and pain. Physical examination showed a mobile, non-tender, abdominopelvic mass. Radiological examination revealed a cystic mass with mixed echogenicity. Histopathological review disclosed a primary malignant melanoma arising in an ovarian mature cystic teratoma. The...
Source: Comparative Clinical Pathology - May 2, 2017 Category: Pathology Source Type: research

Clinicopathological impact of CD20 expression in childhood B cell precursor acute lymphoblastic leukemia (BCP-ALL)
This study aimed to evaluate the efficacy of CD20 expression as a biological marker in BCP-ALL. For 104 children with newly diagnosed BCP-ALL included in this study, CD20 expression was evaluated by flow cytometry. CD20 was positively expressed in 40 (38.5%) of cases. The CD20positive subgroup had significantly shorter overall survival and disease free survival as compared to the CD20negative one (P = 0.018;
Source: Comparative Clinical Pathology - May 1, 2017 Category: Pathology Source Type: research

RAPD-PCR analysis of Staphylococcus aureus strains isolated from different sources
AbstractDetermination of genetic relationship amongStaphylococcus aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine the molecular diversity ofS. aureus strains isolated from human, bovine, and food samples using random amplified polymorphic DNA (RAPD)-PCR method. A total of 208S. aureus strains including 60 human (patients and healthy carriers), 83 bovine milk, and 65 food (pastry and cheese) isolates were assessed. The isolates were confirmed to be methicillin-resistance/susceptibleS. aureus (MRSA or MSSA) using co...
Source: Comparative Clinical Pathology - April 27, 2017 Category: Pathology Source Type: research

Significance of cardiac and iron profile alteration in diabetic patients
This study was conducted on 120 subjects. Subjects were divided into two main groups (80 diabetic patients and 40 healthy controls); diabetic subjects were subdivided into two subgroups (G-IIa and G-IIb) according to fasting blood glucose and glycosylated hemoglobin (HbA1c). Serum iron profile (iron, total iron binding capacity, and percentage transferrin saturation) is calculated as the index of iron. Lactate dehydrogenase (LDH), total creatine kinase (CK), and creatine kinase-MB (CK-MB) activities are measured as an index of cardiac functions. Also, fasting blood glucose and HbA1c were assayed. Diabetic patients were cha...
Source: Comparative Clinical Pathology - April 26, 2017 Category: Pathology Source Type: research

Phyllodes tumors are an uncommon differential diagnosis of axillary mass
AbstractPhyllodes tumors are rare fibroepithelial neoplasm that account for less than 0.5% of all breast tumors and presentation in ectopic breast tissue is even rarer. In this paper, we describe a case of a phyllodes tumor in the ectopic breast tissue of the axilla. A 40-year-old woman presented with a well-circumscribed, firm, and freely mobile mass in the left axilla from 1  year ago. Ultrasound revealed a 5.5 × 4.5 × 4.5 cm homogenous and hypoecho lesion. The histopathologic analysis revealed that it was a benign phyllodes tumor. Phyllodes tumors in ectopic breast tissue may be...
Source: Comparative Clinical Pathology - April 25, 2017 Category: Pathology Source Type: research

Kinetics of inhibitory action of Myrothamnus flabellifolius Welw. on the activities of α-amylase and α-glucosidase
AbstractRecently, efforts have been geared towards the development of alternative medicines to manage diabetes mellitus by searching for pharmacological agents with the ability to retard glucose absorption through inhibition of α-amylase and α-glucosidase. The present study investigated the in vitro antioxidant capability and inhibitory potential ofMyrothamnus flabellifolius leaf extracts on the activities of α-amylase and α-glucosidase. The hydroethanol extract elicited significant α-glucosidase inhibitory effect (IC50 = 0.50 ± 0.04 mg/mL) and a mild α-am...
Source: Comparative Clinical Pathology - April 20, 2017 Category: Pathology Source Type: research

Nicorandil reverses the behavioral changes and brain oxidative stress in a model of schizophrenia induced by ketamine in mice
We examined the effect of the KATP channel opener nicorandil and the KATP channel blocker glibenclamide on the behavioral and biochemical responses to ketamine in mice. Ketamine was injected at 30  mg/kg intraperitoneally (i.p.) daily for 2 weeks, and mice were then treated with either saline, glibenclamide (5 mg/kg), nicorandil (1 or 5 mg/kg), sucrose 1 g/kg, or glibenclamide + sucrose 1 g/kg, i.p. daily for another 8 days. The control group received only saline. Data indicated that ( i) ketamine increased locomotion, rearing, and grooming behaviors. Locomotor activity increased in the c...
Source: Comparative Clinical Pathology - April 20, 2017 Category: Pathology Source Type: research

Potential pathogens in infertile male dromedary camels and their association with the spermiogram and clinical findings
AbstractThe role of five pathogens potentially implicated in the reproductive failure of 141 male dromedary camels was investigated. Breeding soundness examinations, which included semen analysis and testicular histopathology of the infertile male camels, were compared with seven camels of normal fertility. Antibodies againstChlamydia abortus (C. abortus) andToxoplasma gondii (T. gondii) were detected using the ELISA assay. Antibodies againstTrypanosoma evansi (T. evansi) were identified using the card agglutination test. The Rose Bengal plate agglutination test was used to detectBrucella species. Preputial swabs were cult...
Source: Comparative Clinical Pathology - April 18, 2017 Category: Pathology Source Type: research

Relation between diarrhea and infection by protozoans in dairy calves
In this study, newborn calves were selected from farms with history of protozoa infections and diarrhea. A total of 26 calves were used in the study, 18 were positives forGiardia spp., 17 forEmeria spp., and 21 forCryptosporidium spp., considering single and mixed infections. Furthermore, the survival rate of calves due to the presence of diarrhea was significantly different compared to the group without diarrhea, for all three protozoa. In addition, according to this study, it is possible to indicate that the occurrence of diarrhea is highly correlated to the presence ofGiardia sp., leading to economic losses due to a low...
Source: Comparative Clinical Pathology - April 18, 2017 Category: Pathology Source Type: research

Some haematological parameters of albino mice experimentally inoculated with Trypanosoma brucei brucei after administration of Halofantrine hydrochloride, chloroquine and diaminazine aceturate
AbstractTrypanosomosis has been a menace. When animals become infected with trypanosomosis, their physiology is altered leading to haematological aberrations. The comparative effects of Halofantrine chloride (Halfan), chloroquine and diaminazine aceturate (DA) were experimentally investigated in the treatment ofTrypanosoma brucei brucei infected albino mice. This is to know whether Halfan and chloroquine can provide alternatives to diaminazine aceturate. White albino mice experimentally infected withT. brucei brucei which were separately treated 7  days post infection with Halfan at 100 mg/kg body weight as a sin...
Source: Comparative Clinical Pathology - April 14, 2017 Category: Pathology Source Type: research

The evolving hemostatic profile of patients with myeloma receiving treatment
AbstractThrombosis is a well-described complication of myeloma and the drugs used to treat it. The causes are multifactorial. Traditional screening tests of coagulation, such as the prothrombin time and the activated partial thromboplastin time, are known to be poor at assessment of thrombosis risk. It has been suggested that global measures of haemostasis, such as thromboelastography, may have a role to play in the assessment of this risk. The aim of this study was to look at traditional measures of haemostasis, that measure certain parts of the coagulation cascade, as well as thromboelastography, to see if a coagulation ...
Source: Comparative Clinical Pathology - April 11, 2017 Category: Pathology Source Type: research

Effects of short-term repeated fasting on anesthesia: an experimental animal study
The objective of this study was to examine the effect of short-term repeated fasting on some anesthetic parameters of ketamine and xylazine, two anesthetics routinely used in rodents. We divided 50 male mice equally into a fasting and a control group. The fasting group was fasted for 4 continuous days every 2  weeks, and this cycle was repeated four times. During the fasting periods, the fasting mice had access to water only, whereas the control group had free access to both water and food. The mice were allowed a week to recover, then each mouse was anesthetized with a ketamine and xylazine (100 mg/kg and 5 ...
Source: Comparative Clinical Pathology - April 10, 2017 Category: Pathology Source Type: research