Comparing hematological coagulation and biochemical parameters of healthy dogs living at below and above sea levels
AbstractThere is limited information in the literature about the effect of altitude on the variability of hematological, coagulation, and biochemical parameters in healthy dogs. The main aim of this research is to evaluate and compare hematological, coagulation, and selected biochemical parameters of apparently healthy dogs living below and above sea level. Hematological coagulation and selected biochemical parameters were compared between 21 apparently healthy middle-aged dogs living at 180 to 260  m below sea level and 1012 to 1160 m above sea level. The mean values of WBC, MCV, MCH, granulocytes, AST, BUN, sod...
Source: Comparative Clinical Pathology - November 18, 2016 Category: Pathology Source Type: research

Detection of pancoronavirus using PCR in Camelus dromedarius in Iran (first report)
AbstractCoronaviride is a colossal family of viruses that cause a variety of diseases in humans and other animals. As of late, a novel coronavirus, not anterior-optically discerned in humans, has been identified in a denizen of the Middle East. There is growing evidence that theCamelus dromedarius is host species for the virus and plays an important role of a source of human infection. Along these lines, the authors decided to detect coronaviruses in dromedary camels in two high-risk areas of Iran by employing an reverse transcription polymerase chain reaction (RT-PCR) assay. In the present study, nasal swab specimens were...
Source: Comparative Clinical Pathology - November 18, 2016 Category: Pathology Source Type: research

The prevalence and control of bovine mastitis in Leptospira outbreak
AbstractLeptospirosis is considered as one of the most widespread zoonotic disease among many species of wild and domesticated animals, including dairy cows worldwide. Due to its presence and proliferation capacity in the lactating mammary gland, leptospires-related mastitis and milk drop syndrome occur in lactating dairy cows. The aims of this study were to evaluate 1) the occurrence of mastitis due to leptospirosis and 2) the differences ofLeptospira mastitis occurrence between primiparous and multiparous cows. Leptospirosis occurred in a large industrial dairy cattle herd complex in the northwest of Iran for 2  yea...
Source: Comparative Clinical Pathology - November 12, 2016 Category: Pathology Source Type: research

Cupressus sempervirens extract inhibited human basal cell carcinoma tumorigenesis, local invasion, and angiogenic property
AbstractBasal cell carcinoma, a noninvasive and rarely metastatic tumor, correlates  with clinical and histological involvement of basal epithelial cells. It occurs due to dysregulation of hedgehog-patched1 signaling pathway. The current study was conducted to evaluate the in vitro cytotoxic effects ofCupressus sempervirens methanolic extract against primary basal cell carcinoma cells, over a period of 48  h. We measured protein level of Annexin-V and the leakage rate of lactate dehydrogenase in cells being exposed to 420 μM extract. In addition to transcript levels ofPTCH-1,  a recept...
Source: Comparative Clinical Pathology - November 12, 2016 Category: Pathology Source Type: research

Capillary refill time, bleeding time, clotting time, erythrocyte sedimentation rate and prothrombin time in natural cases of canine Trypanosoma congolense infection
AbstractTrypanosomiasis of man and his domestic animals continues to pose enormous public health and economic problems in many parts of Africa and South America. Thirty-one dogs were used for this study. 23 of the dogs were apparently healthy while eight were confirmed to be suffering fromTrypanosoma congolense. Confirmation of trypanosomiasis was done by wet mount and by the use of Dip Quick ™ stain. Capillary refill time, bleeding time, clotting time, erythrocyte sedimentation rate and prothrombin time were determined. The mean values for the apparently healthy dogs were 1.32 s (seconds), 3.34 min (minute...
Source: Comparative Clinical Pathology - November 12, 2016 Category: Pathology Source Type: research

Bilateral ovarian pedicle ligation as an alternative to ovariectomy and ovarian response to eCG treatment
In conclusion, the results of the present study showed that despite the severe changes in ovarian tissue following ovarian pedicle ligation, minor perfusions still exist and supply the ovaries so that hormone response and follicular development can occur. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 10, 2016 Category: Pathology Source Type: research

Retraction note to: Prevalence of the C282Y and H63D mutations of the HFE hemochromatosis gene in Azerian major β-thalassemia and iron overload
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 4, 2016 Category: Pathology Source Type: research

Second-degree atrioventricular block in a diarrheic calf-camel ( Camelus dromedarius )
AbstractAtrioventricular block (AVB) is a type of heart block in which the conduction between the atria and ventricles of the heart is impaired. The purpose of the present paper is to report the occurrence of second-degree AVB in a diarrheic calf-camel. A 7-day-old female calf-camel (Camelus dromedarius) weighting approximately 50  kg was referred to the veterinary hospital with a 5-day history of diarrhea, prostration, tremors, sternal recumbency, and muscular weakness. Clinical examination revealed increases in rectal temperature (RT 39.9 °C), heart rate (HR 124 beats min−1), and respiratory rate (RR ...
Source: Comparative Clinical Pathology - November 4, 2016 Category: Pathology Source Type: research

Clinicopathological and microbiological findings associated with wounds in Nigerian horses
This study was a 6-month survey of wound infections of horses at the Obollo-Afor Horse Lairage, Udenu Local Government area, Enugu State, Nigeria. Two hundred seven horses were sampled. They were physically examined, and those that had wounds were purposively selected for further study. Blood samples were collected from these horses that had wounds for haematological and serum biochemistry evaluations. Sterile swab sticks were used to collect samples from the wound edges after disinfection for microbiological examination. This showed that out of the 207 horses sampled, 21 had wounds. The horses with wounds had significantly (p 
Source: Comparative Clinical Pathology - November 4, 2016 Category: Pathology Source Type: research

Hematological and biochemical values in Brazilian four-beat gaited horses
AbstractHorse selection is still associated with gait type, and this aspect is important for four-beat gaited horses, a large group that is distributed around the globe with more than 140 breeds. However, there is little information about their hematological and biochemical profiles, and such information will contribute to improving their exercise and nutritional evaluation programs. The aim of this study was to determine the hematological and biochemical values of athletic Campolina (CAMP) and Mangalarga Marchador (MM) horses performing two different types of gait. Overall, 100 adult horses (53 males and 47 females) with ...
Source: Comparative Clinical Pathology - November 3, 2016 Category: Pathology Source Type: research

Evaluation of the correlation of automated and manual results of complete blood count in oncologic patients
AbstractThe aim of this study is to evaluate the performance of Sysmex XS-800i analyzer and compare its correlation with standard manual method in oncologic patients. The complete blood count (CBC) with differential leukocyte count is one of the most frequently requested hematologic tests. In oncologic patients, it is more important because primary diagnosis and treatment follow-up are depending on CBC results. Automated hematology analyzers are designed to perform CBC with acceptable precision and accuracy, but manual slide examination still is needed to complement the analyzer results. One thousand CBC samples of oncolog...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Effect of human amniotic membrane on anastomosis leakage in dog model
AbstractOne of the major concerns in rectal cancer surgery and complicated anastomosis is anastomosis breakdown and leakage. For decades, radiation (as neoadjuvant or adjuv ant therapy) was known as a risk factor for anastomosis leakage. Diverting loop ileostomy has been usually employed to divert the fecal flow and protect complicated anastomosis. However, there is a high rate of complications related to diverting loop ileostomy and its closure. This experimental study was carried out to investigate the effect of human amniotic membrane on colonic anastomosis in dogs that underwent preoperative radiation therapy. In this ...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Antimicrobial effects of new designed 2-amino-tetrahydro-4 H -chromene-3-carbonitrile derivatives against some pathogenic microbial strains
AbstractChromenes, also called benzopyrane derivatives and chromene-4-one, are natural compounds which have several biological effects. The aims of the present study were to synthesize 2-amino-tetrahydro-4H-chromene-3-carbonitrile derivatives and to evaluate their antibacterial effects against selected bacterial strains. Nine 2-amino-tetrahydro-4H-chromene-3-carbonitrile derivatives were designed and synthesized. Each synthesized derivative was dissolved in dimethyl sulfoxide and diluted using distilled water. Then, serial dilutions of 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, and 0.125  μg/ml were prepared and added to t...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

A case of feline panleukopenia in Felis silvestris in Iran; confirmed by PCR
In conclusion, current case report presents FPL inFelis silvestris for the first time in Iran. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Effect of Citrullus vulgaris on some functional indices of selected tissues of Wistar rats
This study aimed at evaluating the implications of such consumption. Study involved the use of 35 Wistar rats grouped into seven with a control group administered distilled water, and the other groups administered varying concentrations of the pulp and whole fruit for 28  days. Alanine and aspartate aminotransferase (ALT, AST) were assayed in the serum and liver. Total protein of the serum and selected tissues was determined and malondialdehyde (MDA) level measured. Phytochemical screening revealed the presence of steroids, saponin, alkaloids, flavonoids, and terpe noids in both whole fruit and pulp. Significant increases (p 
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Slaughterhouse report of intrahepatic cholangiocarcinoma in a Holstein cow
AbstractCholangiocarcinoma (CCA) is a primary tumor of the bile duct epithelium. Cholangiocarcinoma is relatively uncommon in domestic animals and the previous reports are documented more in dogs and cats, and less in other species. The current study described intrahepatic CCA in a 6-year-old female Holstein cow. Grossly, multiple parenchyma were scattered throughout the liver parenchyma. Histopathologically, the tumoral masses were composed of gland-like, tubular, acinar structures or solitary islands that embedded in the abundant connective tissue stroma with a cuboidal to columnar epithelial cells. Polymorphism and mito...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Effect of ionophore salinomycin on the antibiotic resistance in Clostridium difficile detected in mice
AbstractIn the present study, an in vivo model was established to pursue the possible influence of ionophore, salinomycin, on the mobile resistance genes inClostridium difficile. Fecal samples of 48 healthy adult mice were initially checked for the presence ofC. difficile using a species-specific pair of primers. The animals were subsequently divided into the following experimental groups as follows salinomycin + tetracycline, tetracycline, salinomycin, and control (no treatment), for 10  days. Fecal sample was then collected from each group at days 0, 3, and 5 posttreatment, anaerobically enriched in the thioglycolat...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Effect of methanolic leaf extract of Telfairia occidentalis (Hook f.) on vaginal cytology, serum hormonal levels and ovarian histomorphology of female (Sprague —Dawley) albino rats
This study evaluated the effect of the oral administration of methanolic leaf extract ofT. occidentalis on vaginal cytology, serum hormone (progesterone and estrogen) levels, and histomorphology of the ovaries of female albino rat. Fifteen female albino rats were used for the study. They were randomly assigned to three groups of five rats each. The first group (A) served as the untreated control and received distilled water while the second group (B) and third (C) received 200 and 800  mg/kg body weight of the extract respectively for 21 days. Evaluation of vaginal cytology did not show any effect of the extract ...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Platelet variables in healthy dogs: reference intervals and influence of age, breed and sex
AbstractPlatelet parameters are routinely available and might be valuable in veterinary diagnostics. The aim of this study was to assess influences of age, breed and sex on platelet parameters as well as to define reference ranges for dogs. Data of 166 healthy dogs were reviewed retrospectively. One hundred fifty-six dogs aged>1  month to 8.5 years were categorised into five age groups. Reference intervals were determined for 119 adult dogs and for this subpopulation the influence of sex and neuter status was examined. Breeds withn ≥ 5 individuals were statistically compared; these included 11 Hav...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Measurements of cardiac troponin I and creatine kinase myocardium isoform in dogs with diabetic ketoacidosis
AbstractDiabetic ketoacidosis (DKA) is a complication of diabetes mellitus (DM) which may cause cardiac injury. The study aimed to compare concentrations of cardiac troponin I (cTnI) and creatine kinase myocardium isoform (CK-MB) in DKA and normal dogs and determine the relationship between cTnI and CK-MB concentrations and blood profile values, complications, and the risk of death. Plasma samples from 20 normal and 24 DKA dogs were collected. The cTnI concentration of DKA dogs (0.053 [0.034 –0.200] ng/ml) was higher than those of normal dogs (0.020 [0.015–0.043] ng/ml) (p 
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Study of green muscle disease in some condemned broiler chicken from Iran
AbstractThe study was descriptively conducted to determine incidence of green muscle disease (Oregon disease) in slaughtered broiler chickens from west of Iran. During the study, five million broiler chickens were slaughtered out of which 29,255 (0.53  %) carcasses were condemned. Almost 1850 (0.033 %) carcasses were diagnosed with green muscle disease. The lowest and highest rate of the disease were seen in August (6.6 %) and February (14.2 %), respectively. There were significant relationships between chicken’s age, weight, and occurrence of the disease (P 
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Comparative assessment of antibiotic potency loss with time and its impact on antibiotic resistance
AbstractA wide range of diverse antibiotics is available in the current world ’s drug market, which is mostly being used for the treatment of infectious diseases in humans as well as in veterinary. A large number of new antibiotics are continuously being added to the existing list. However, at the same time, most of the pathogens have tended to overcome antibiotic stress du e to their specific mutations and adaptations with time. Hence, several antibiotics turn to be non-effective in the long run. Moreover, antibiotics also lose their effectiveness with time as they approach their expiry period. The goal of this stud...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Claudin-17 reduced expression is associated with high histologic grade and shorter overall survival in canine mammary carcinoma
AbstractThe present study investigates Claudin-17 expression in canine mammary carcinomas by means of immunohistochemistry and its relationships with histologic grades, overall survival and tumour recurrence. Our results show that Claudin-17 expression is reduced significantly in canine mammary carcinomas compared with normal canine mammary tissues. High histologic grade and shorter overall survival have been shown to be significantly associated with Claudin-17 reduced expression. Results of this study suggest a possible relationship between reduction of Claudin-17 and canine mammary carcinoma pathogenesis. (Source: Compar...
Source: Comparative Clinical Pathology - October 17, 2016 Category: Pathology Source Type: research

Gastroprotective potential of melatonin versus melatonin loaded niosomes on gastric ulcer healing in rats
AbstractThe present study was conducted to investigate the therapeutic effects of melatonin and melatonin-loaded niosomes on ethanol-induced gastric lesions. Morphological and biochemical changes associated with each drug were investigated. Seventy apparently healthy female albino rats, weighting about 200 –250 g were randomly assigned to 4 experimental groups. The first group (induction group) included 10 rats that were given 1 ml 70 % ethanol and sacrificed after 1 and 1/2 hour. The second group (melatonin-treated group) included 25 rats that were administrated with 2 mg/rat melatonin by gastr i...
Source: Comparative Clinical Pathology - October 14, 2016 Category: Pathology Source Type: research

Effects of dietary supplementation of Lactobacillus Plantarum on survival, hematological profile and histopathology of selected organs of Clarias gariepinus fingerlings
This study evaluates the effects of dietary supplementationof Lactobacillus plantarum (LP) on survival of hematological profile and histopathology of selected organsof Clarias gariepinus fingerlings. TenClarias gariepinus fingerlings were allocated to five treatments, with three replicated subgroups each, fed and reared for 12  weeks.LP isolated from corn slurry was cultured using standard procedures. Five iso-nitrogenous diets were formulated at 35  % crude protein (T0, T1, T2, T3, and T4) withLP at inoculation rate of 0  × 107 (T0), 0.5  × 107 (T1), 1.0  × 107 (T2...
Source: Comparative Clinical Pathology - October 13, 2016 Category: Pathology Source Type: research

Proteomic impacts of electromagnetic fields on the male reproductive system
AbstractThe use of mobile phones and other wireless transmitting devices is increasing dramatically in developing and developed countries, as is the rate of infertility. A number of respected infertility clinics in Australia, India, USA, and Iran are reporting that those who regularly use mobile phones tend to have reduced sperm quantity and quality. Some experimental studies have found that human sperm exposed to electromagnetic fields (EMF), either simulated or from mobile phones, developed biomarkers of impaired structure and function, as well as reduced quantity. These encompass pathological, endocrine, and proteomic c...
Source: Comparative Clinical Pathology - October 13, 2016 Category: Pathology Source Type: research

Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
In this study, 75 Sprague-Dawley rats were divided into 3 groups of 25 rats, which were further subdivided into 5 subgroups each. Group 1 (negative control) was given distilled water, group 2 (positive control (PC)) administered 10  mg/kg of lead acetate (LA) orally/daily, while groups 3 (T1), 4 (T2), and 5 (T3) were given LA 10 mg/kg and graded concentrations (100, 150, and 200 mg/kg) each of NS. One group each, comprising of 25 rats, was euthanized at days 30, 60, and 90 for collection of blood plasma, epididymis, and org an tissues fixed in 10 % buffered formalin at each time interval. The right caud...
Source: Comparative Clinical Pathology - October 12, 2016 Category: Pathology Source Type: research

Evaluation of the healing effect of honey and colostrum in treatment of cutaneous wound in rat
In this study, 80 adult female Sprague Dawley rats were randomly divided into four groups as negative control group, groups II, III, and IV were treated by honey, bovine colostrum, and honey + colostrum, respectively. Rats were anesthetized, hair was removed from the back, and thena wound was made on the back. Visual observation, histopathological examination, and biomechanical study were performed on days 3, 7, 14, and 21 after operation. Wound area in rats which taken colostrum and honey was lower than other groups. Promotion of wound contraction and epithelialization in rats that were treated with honey and colostrum wa...
Source: Comparative Clinical Pathology - October 11, 2016 Category: Pathology Source Type: research

Efficacy of ARACNE algorithm for inferring canine B-cell lymphoma gene regulatory network (GRN)
AbstractLymphoma is the most frequent hematopoietic cancer in dogs. Canine B-cell lymphoma has been proposed as an ideal model of human non-Hodgkin ’s lymphoma (NHL). Critical genes playing important roles in the cancer progression can be detected using the reconstruction and analysis of gene regulatory network (GRN). GRNs are inferred using various computational algorithms, whereARACNE is on the most important and efficient algorithms. Here, we evaluated the efficacy ofARACNE to reconstruct canine B-cell lymphoma GRN via different computational analyses. Hence, the gene expression profile of GSE43664 was downloaded ...
Source: Comparative Clinical Pathology - October 11, 2016 Category: Pathology Source Type: research

Pathological and diagnostic investigations of abortions and neonatal mortality associated with natural infection of Brucella abortus in dromedary camels
This study was carried out to diagnose and investigate the pathological lesions associated withBrucella abortus in the fetuses and placenta of naturally infected dromedary camels. For diagnosis, the blood samples from infected dams were subjected to Rose Bengal Plate Test (RBPT) and blood PCR. The placental and fetal lung tissue samples were subjected to pathological examination and PCR. Nine cases of abortions and one case of early neonatal mortality was reported in the infected camels. The hematological parameters in infected dams revealed anemia and leukocytosis. The important pathological lesions in the infected fetuse...
Source: Comparative Clinical Pathology - October 10, 2016 Category: Pathology Source Type: research

The effect of Portulaca oleracea (purslane) seeds on hemoglobin levels in adolescent girls with iron deficiency anemia: a randomized comparative trial
AbstractIron deficiency anemia (IDA) is the most severe and the latest stage of iron deficiency. Consumption of purslane seeds is a traditional medicine approach for the treatment of anemia. We aimed to compare the effect of purslane seeds and ferrous sulfate (FS) on the hemoglobin (Hb) level of adolescent girls suffering from IDA. In this single-blind randomized comparative clinical trial, the effect of purslane seeds was compared with a FS tablet in 53 adolescent girls. Included subjects were within the age range of 15 –18 years; with Hb levels of 10–12 g/dL and serum ferritin levels lower than 12&n...
Source: Comparative Clinical Pathology - October 10, 2016 Category: Pathology Source Type: research

Chemokine receptor ’s expression in human adipose derived mesenchymal stem cells primed with valproic acid
AbstractAmong stem cells, mesenchymal stem cells (MSCs) are ideal for practical regenerative medicine and among MSCs, adipose-derived (Ad)-MSCs are the best sources for this regard. Migration or homing to the injured tissue is the main property of MSCs which is related to chemokine receptors on their cell membrane and could be affected by some materials such as valproic acid (VPA). The present study investigated whether VPA could increase the expression of these receptors. In doing so, human Ad-MSCs were isolated from adipose tissue (AT), and after primary culture and characterization, the cells in passage four were primed...
Source: Comparative Clinical Pathology - October 8, 2016 Category: Pathology Source Type: research

Molecular investigation of Anaplasma and Ehrlichia natural infections in the dromedary camel ( Camelus dromedarius ) in Iran
This study describes the first molecular prevalence of CandidatusA. camelii in Iranian dromedary camels. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - October 8, 2016 Category: Pathology Source Type: research

Hematological and histopathological assessment of pacu ( Piaractus mesopotamicus ) after treatment of pathogens with veterinary medicinal products
This study performed hematological and histopathological evaluations of pacu (Piaractus mesopotamicus) that had been naturally infected withAeromonas sp.,Streptococcus sp.,Ichthyophthirius multifiliis,Trichodina heterodentata, andAnacanthorus penilabiatus and treated with enrofloxacin and toltrazuril or with florfenicol and thiamethoxam. After 7  days of treatment from nine fishes were collected blood, via caudal puncture, and samples of the gills, liver, and kidneys. Following toltrazuril and enrofloxacin treatment, fish exhibited leukocytosis with lymphocytosis. With thiamethoxam and florfenicol treatment, the fish ...
Source: Comparative Clinical Pathology - October 7, 2016 Category: Pathology Source Type: research

The effect of curcumin (Curcuma longa L.) on circulating levels of adiponectin in patients with metabolic syndrome
AbstractIn modern societies, metabolic syndrome (MetS) is a highly common disease, which is closely associated with the increased risk of cardiovascular disease (CVD). Metabolic syndrome is usually accompanied by low levels of adiponectin, which is a key regulator of insulin sensitivity and reduced inflammation of the tissue. Adiponectin also reduces systemic insulin resistance and predicts cardiovascular disease. Curcumin has several beneficial effects on risk factors of metabolic syndrome. This polyphenol can affect almost all components of metabolic syndrome including insulin resistance, hypertension and obesity. Accord...
Source: Comparative Clinical Pathology - October 7, 2016 Category: Pathology Source Type: research

High platelet count and high probability of CALR detection in myeloproliferative neoplasms
In this study, we used PCR-PAGE and DNA sequencing methods for detection of CALR mutations and analysis of laboratory findings in MPN patients. BCR-ABL1, JAK2V617F, andMPL515 were assessed using RT-PCR and ARMS-PCR methods in 103 suspected MPN patients. CALR was assessed in patients negative for BCR-ABL1 and JAK2V617Fpatients as well as six cell lines (Jurkat, U937, NB4, KCL22, THP1, and K562) using PCR-PAGE and DNA sequencing. Finally, laboratory data of patients were obtained. BCR-ABL1 and JAK2V617F were detected in 39 (37.86  %) and 34 (33 %) MPN patients, respectively. Two patients had combined BCR-ABL1/JAK2V...
Source: Comparative Clinical Pathology - October 4, 2016 Category: Pathology Source Type: research

Erratum to: The effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 28, 2016 Category: Pathology Source Type: research

Fungal flora in the mouth of venomous and non-venomous snakes
This study was conducted to identify potentially pathogenic fungal flora in the oral cavities of venomous and non-venomous snakes. Sterile swabs were used to retrieve samples from the oral cavities of different snakes followed by medium-specific culture. Slide culture technology was also used in the identification of fungal flora.Rhizopus andMucor represented the most common fungal flora each making up 29  % of the total.Alternaria, Cladosporium, Aspergillus andCandida represented a significantly smaller portion of fungal pathogen load at around 3  % each. Fungi, Yeast (19.4 %) and Penicillium (9.7 %), ...
Source: Comparative Clinical Pathology - September 27, 2016 Category: Pathology Source Type: research

CD63-Alix-Rab27a exosome axis is identically influenced in Chediak-Higashi syndrome
AbstractChediak-Higashi syndrome is characterized by congenital abnormality in exocytosis activity, and accumulation of intracellular lysosome-like structures different types of cells within the body. Accordingly, the lack of appropriate exocytosis and deficiency in lysosomal trafficking accounts for immune-related hypoactivity. Exosomes are other types of cell-derived vesicles that play key role in cell-cell cross talk. However, the possible effect ofLYST gene mutation in exosome trafficking and biogenesis remains to be clarified. Herein, we investigated the expression of CD63-Alix-Rab27a exosome axis in Chediak-Higashi s...
Source: Comparative Clinical Pathology - September 27, 2016 Category: Pathology Source Type: research

The Semelil (ANGIPARS ™) ameliorates cardiac functional disturbances after experimental chronic myocardial infarction model in rabbit
AbstractMyocardial infarction (MI) is one of the important causes of mortality in all populations. Some herbal drugs have been utilized for the treatment of MI. This research was designed to study the cardioprotective effects ofSemelil (ANGIPARS ™), a novel herbal drug, in male rabbits. Twenty New Zealand white rabbits were randomly allocated into four groups: control vehicle, sham vehicle, ischemia vehicle, andSemelil 10  mg/kg. Ischemia was created by complete occlusion of the left anterior descending coronary artery (LADC).Semelil was intraperitoneally injected for 2  weeks in the treatment group. Functi...
Source: Comparative Clinical Pathology - September 23, 2016 Category: Pathology Source Type: research

Genotypic analysis of Giardia duodenalis in children at Egypt
AbstractGiardia duodenalis is among the most common and frequent intestinal protozoan infecting Egyptian children. The present study aims to identify the genotypic features ofG. duodenalis among children fecal samples complaining of diarrhea at Beni-Suef Governorate, Egypt, and to study the association betweenGiardia assemblages and clinical presentation of the disease among this category. One hundred thirty diarrheic stool samples were subjected to direct stool examination, and positive samples forGiardia were subjected to copro-DNA extraction and amplification targeting the triose phosphate isomerase (tpi) gene using nes...
Source: Comparative Clinical Pathology - September 23, 2016 Category: Pathology Source Type: research

Correlation between γ-globulins and Encephalitozoon cuniculi immunoglobulins in suspected infected rabbits
In this study, 76 symptomatic rabbits were divided in three groups based on their Ig results: IgM+ IgG+, IgM-IgG+ and IgM-IgG-. By statistical analysis total serum protein was proved not to be effective in predicting an antibody response, in contrast the γ-globulin ranges from serum electrophoresis can predict whether the patient is positive for IgM and/or IgG. In fact, a significant quantitative correlation between percentage of γ-globulins and positive IgM and IgG serum forE. cuniculi was established in symptomatic rabbit. These values can be useful to perform a screening both for symptomatic and asymptomatic...
Source: Comparative Clinical Pathology - September 22, 2016 Category: Pathology Source Type: research

Neem ( Azadirachta indica ) leaf and kernel and their effects on production and physiology of poultry: a review
AbstractThe heavy demand for cereal grains and legumes by industries, humans and animals has given rise to increase in their prices and consequently in the costs of livestock feeds. There is a need therefore for the exploitation of other alternative noncompetitive feed resources in other to replace cereal grains and legumes in poultry diets. Many tropical plants produce leaves and seeds that are little known in poultry nutrition. The identification, processing and use of such leaves and seeds in poultry diets will go a long way in reducing the dependence on conventional feedstuffs. Neem is a tropical tree plant that yields...
Source: Comparative Clinical Pathology - September 17, 2016 Category: Pathology Source Type: research

Alkaloid extracts from shea butter and breadfruit as potential inhibitors of monoamine oxidase, cholinesterases, and lipid peroxidation in rats ’ brain homogenates: a comparative study
AbstractShea butter (Vitellaria paradoxa) and breadfruit (Treculia africana) are wild edible plants commonly consumed across the world. Unfortunately, these plants are currently going into extinction despite their potential biological properties. The actions of alkaloid extract from shea butter nut and breadfruit on some central nervous system biomarkers (monoamine oxidase (MAO), acetylcholinesterase (AChE), butyrylcholinesterase (BChE)) linked to some neurodegenerative diseases were investigated in this study. The radical scavenging and metal chelating abilities of the extract were also studied. Alkaloid extracts were obt...
Source: Comparative Clinical Pathology - September 9, 2016 Category: Pathology Source Type: research

Coexisting of titanium dioxide nanoparticles and diazinon on histopathology of common carp ( Cyprinus carpio )
In conclusion, this research suggested that the coexistence toxicity of diazinon and TiO2 NPs on the gill and intestine histopathology of common carp was higher than the diazinon and TiO2 NPs alone. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 7, 2016 Category: Pathology Source Type: research

The phylogenetic similarity of hydatid cyst isolated from humans and sheep in Ilam Province southwest of Iran
AbstractHydatid cyst is a chronic zoonotic disease caused by the larval stage of the dog tapeworm,Echinococcus granulosus. To identify genotype of hydatid cysts of human and sheep jackal in Ilam Province (South West of Iran), the PCR-RFLP and DNA sequencing were used. A total of 10 human and 20 sheep protoscoleces hydatid cyst samples were collected from different hospitals and slaughterhouses. Then, the gene of cox1 of mitDNA of the parasite was amplified and PCR products were cut usingAluI and HpaII restriction enzymes. Finally, a number of PCR products were bi-directionally sequenced. Based on the DNA sequencing and PCR...
Source: Comparative Clinical Pathology - September 6, 2016 Category: Pathology Source Type: research

Physicochemical and structure-activity properties of piroxicam —a mini review
AbstractThe neutral bidentate ligand coordinated to the metal ion through pyridyl-N and carbonyl of the amide moiety causes protonation/deprotonation equilibria (tautomerism) of piroxicam. The penetration of piroxicam through blood –brain barrier (BBB) may be by redox chemical delivery system linking it to the lipophilic dihydropyridine carrier creating a complex with carboxylic acid that transverses the BBB. The complex is enzymatically oxidized to the conic pyridinium salt. Subsequent cleavage of the drug from the pyridini um carrier leads to sustained drug delivery in the brain parenchyma. Brain uptake of piroxica...
Source: Comparative Clinical Pathology - August 12, 2016 Category: Pathology Source Type: research

Dietary docosahexaenoic acid to eicosapentaenoic acid ratios effects on hemato-immunological and plasma biochemical parameters in silvery-black porgy ( Sparidentex hasta ) juveniles
AbstractAn 8-week feeding experiment was conducted in juvenile silvery-black porgy (Sparidentex hasta) (initial mean weight 20.1  ± 0.1 g, mean ± SD) to estimate the optimum dietary docosahexaenoic to eicosapentaenoic acid (DHA/EPA) ratio. Triplicate groups of fish (12 fish in each replicate) were kept into 15 cylindrical polyethylene tanks (250 L) at 18.7 °C and handfed by one of the five diets which formulated wit h graded ratios of DHA/EPA (0.2, 0.4, 0.9, 2.0, 3.3) two times daily. In the fillet and liver, DHA and DHA/EPA ratios significantly increased with increasing...
Source: Comparative Clinical Pathology - August 10, 2016 Category: Pathology Source Type: research

Diffuse type testicular seminoma in a stallion
AbstractA 7-year-old stallion with progressive left testicular enlargement was presented. Grossly, the excised testicle measured 25  × 15 × 12 cm and weighed 3.7 kg. It was multinodular with a gray-white surface; however, the right testis was normal. Histologically, the neoplastic cells were disseminated diffusely in the tumoral stroma with a minimal fibrovascular stroma. Neoplastic cells were round to polygonal with abu ndant eosinophilic cytoplasm and large round to oval vesicular or hyperchromatic nucleous with a single prominent nucleolus. Immunohistochemically, the neoplastic ...
Source: Comparative Clinical Pathology - August 9, 2016 Category: Pathology Source Type: research

Some hematological reference values estimated by the reference values advisor in the Iraqi Awassi sheep
AbstractThe hematological parameters in domestic animal can be affected by several factors such as the sex, age, breed, and nutrition. There is a few published data on hematological reference values of the Iraqi Awassi sheep. Hence, the study aimed to determine hematological reference interval (RI) for the Awassi sheep to establish a basis for clinical interpretation. In the present study, a total 130 healthy Awassi sheep for both sex with two ages (2 –5 years) were selected randomly from five regions (Shula, Abu-Ghraib, Mahmoudia, Yousifia, and Alameel) West and South of Baghdad during the period from December ...
Source: Comparative Clinical Pathology - August 9, 2016 Category: Pathology Source Type: research