Validation of an immunophenotyping method to characterise of lymphocyte subpopulations in Crl:WI(Han) rat spleen by FACSVerse flow cytometer
AbstractOECD guideline 443 describes the requirements for an Extended One-Generation Reproductive Toxicity Study in the rat. One cohort assesses reproductive/developmental endpoints, including investigation of pre- and postnatally induced immunotoxic effects. The aim of this study was to develop and validate flow cytometry assays to characterise rat spleen lymphocyte subpopulations, and to ensure that accurate and relevant data was obtained that satisfied the guideline requirements. Homogenised spleen samples from Crl:WI(Han) rats were processed for lymphocyte subpopulation evaluation using the FACSVerse flow cytometer. Sa...
Source: Comparative Clinical Pathology - September 29, 2022 Category: Pathology Source Type: research

Effects of exercise preconditioning on NLRP3 and mitochondrial fission in isoproterenol-induced myocardial infarcted rats
AbstractMyocardial infarction is a common disease that causes morbidity and mortality in human. Exercise training is an effective strategy to improve cardioprotection. The signaling pathways of exercise preconditioning on the reduction of MI-induced cardiac injuries is one of the topics that has attracted a lot of attention. The purpose of this study was to investigate the effects of exercise preconditioning on cardiac damage and dynamin-related protein 1 (Drp1) as an effective factor for activating the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) following myocardial infarction (MI). Twenty male Wistar rats w...
Source: Comparative Clinical Pathology - September 23, 2022 Category: Pathology Source Type: research

Cross-sectional study of 100 cases of newly diagnosed plasma cell neoplasms in a tertiary care setup
AbstractThe aim of the study was to evaluate the clinical and laboratory profile of newly diagnosed cases of Multiple myeloma (MM) in a tertiary care hospital in western India. Records of all the patients who were diagnosed as MM was taken out from the archives from September 2013 to February 2021 and analyzed for various parameters. Of the 100 patients studied, 3% were below the age of 40  years with most of the cases in the 6th and 7th decade. The mean age of the study was 61.98  years. The most common symptoms were low backache (LBA), bone pains and easy fatigability. Anemia was present at the outset in 67% of cases, ...
Source: Comparative Clinical Pathology - September 23, 2022 Category: Pathology Source Type: research

Oleuropein reduces the oxidative effects of tobacco smoke in rats ’ liver and kidney
In this study, potential protective effects of OLE on tobacco, smoke-exposed rats were evaluated. Eighteen male Sprague Dawley rats were divided into 3 groups: Control, Tobacco smoke, Tobacco smoke  + OLE. The rats in tobacco smoke and tobacco smoke + OLE were exposed to tobacco smoke in a glass chamber for 1 h every other day for 12 weeks. Tobacco smoke + OLE were received 10 mg/kg OLE for the last 4 weeks by oral gavage. After 12 weeks, rats were decapitated; kidney and live r tissues were taken. Malondialdehyde (MDA), glutathione (GSH), NO levels together with catalase (CAT) and arginase activity were m...
Source: Comparative Clinical Pathology - September 20, 2022 Category: Pathology Source Type: research

Effect of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis paniculata (king of bitters) leaves in high sucrose diet-fed fruit flies (Drosophila melanogaster)
This study evaluated the effects of cooking duration on the antioxidant and antihyperglycemic properties ofAndrographis paniculata on high sucrose diet fed fruit flies (Drosophila melanogaster).A. paniculata leaves were pulverized and divided into three portion; one was subjected to water infusion and the other two portions were cooked for 5 and 10  min respectively. Wild-typeD. melanogaster were raised for 14  days on high sucrose diet (HSD) supplemented withA. paniculata at different cooking intervals. Thereafter, the mortality rate, negative geotaxis, body weight, antioxidant indices, thiobarbituric reactive substance...
Source: Comparative Clinical Pathology - September 17, 2022 Category: Pathology Source Type: research

Changes and relations of 25(OH) vitamin D and some inflammatory and oxidative stress indicators in healthy dairy cows during transition period
AbstractDairy cows undergo dramatic metabolic changes and impaired immune system function in the transition period; thus, they are very susceptible to peripartum disease. Vitamin D as an immune-modulatory agent may have protective effects against oxidative stress and numerous diseases. Our objective in this study was to investigate the changes in 25(OH)D concentrations in dairy cattle during the transition period and its association with some inflammatory and oxidative stress markers. Twenty-three healthy cows were selected from two commercial farms and were sampled 1  week prepartum and 1 week and 3 weeks postpartum. 2...
Source: Comparative Clinical Pathology - September 5, 2022 Category: Pathology Source Type: research

Megakaryocyte cytoplasmic fragments and ragocytes in the peripheral blood of a domestic cat infected with Anaplasma
AbstractIn this case report, the author reports three strong and highly unusual changes in the peripheral blood of a domestic cat infected withAnaplasma. A freshly made blood smear from the catAnaplasma had a shower of about 100 variably large to huge cytoplasmic fragments of megakaryocyte cytoplasm along the feathered edge. These had budding of platelets at their margins. Neutrophils with red cytoplasmic inclusions resembling ragocytes and neutrophils with ingested round inclusions of nuclear material were seen in a 1-day-old EDTA blood sample but not the freshly made blood smear. The cells with nuclear material resembled...
Source: Comparative Clinical Pathology - September 3, 2022 Category: Pathology Source Type: research

Immunohistochemistry reveals an increased number of ganglion cells in the normal-size plexus, as a pathological feature of immaturity of ganglia
AbstractImmaturity of ganglia (IG), is a rare entity of an allied disorder of Hirschsprung ’s disease. We reviewed our IG cases to provide further pathological insight into IG. The clinical data and pathological findings of IG cases in our department from 2011 to 2020 were examined. Hematoxylin and eosin (HE) staining and immunostaining for HuC/D, BCL-2, SOX10, and CD56 were performed o n full-thickness specimens. Sufficient clinical data and pathological specimens were available in five cases during the study period. The patient profiles were as follows: four term infants and one preterm infant with initial symptoms of ...
Source: Comparative Clinical Pathology - September 1, 2022 Category: Pathology Source Type: research

Effect of a multi-strains yeast fractions product plus anticoccidial drug on performance, anticoccidial index, intestinal health, and bone mineralization of broiler chickens infected with Eimeria spp
In conclusion, MSYF can be used in combination with salinomycin (COMB) to reduce the negative effects of anEimeria challenge, as a consequence, improve growth performance in the challenged birds. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - August 19, 2022 Category: Pathology Source Type: research

Identification of Anaplasma phagocytophilum infection among camels (Camelus dromedarius) in Yazd, Iran
In this study,A. phagocytophilum was detected by microscopy and molecular examination among 125 male camels (C. dromedarius) in Yazd, central Iran. TheA. phagocytophilum morula was observed in one sample out of 125 (0.8%) in microscopic examination and was detected in 4 samples out of 125 (3.2%) in the molecular examination. According to the results,A. phagocytophilum infects camels (C. dromedarius) consequently, camels can play a role in the infection of ticks, other animals, or humans. Many aspects of this disease continue to be set up by way of further studies in Iran. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - August 19, 2022 Category: Pathology Source Type: research

Phytochemistry of Prunus africana and its therapeutic effect against prostate cancer
AbstractProstate cancer is still one of the world ’s top triggers of mortality. In light of the restricted therapy choices for people with prostate cancer, natural substance-based prevention and therapeutic techniques can contribute significantly to combating this illness.P. africana, a Rosaceae-family evergreen shrub native to Sub-Saharan Africa, has traditionally been used to cure prostate cancer. The focus of this research was therefore to review the phytochemistry ofP. africana against prostate cancer. P. africana extracts produced from various plant sections have been reported to constitute a number of phytochemical...
Source: Comparative Clinical Pathology - August 12, 2022 Category: Pathology Source Type: research

Comparison of the effects of two methods of euthanasia on post mortem changes in rats: histopathological and molecular findings
AbstractPostmortem changes (PMCs) not only affect the results of experimental studies, but also determine postmortem intervals in forensic sciences. The present study aimed to assess the effects of two methods of laboratory animals ’ euthanasia on PMCs in rats. In this experimental study, 10 female rats were randomly assigned to two equal groups and were euthanized using the inhalation of CO2 (gas-treated group) or over-dose intramuscular injection of ketamine/xylazine (drug-treated group). Kidney and liver tissue samples were collected at baseline and 2, 4, 6, 8, 10, 12, 24, and 48  h after euthanasia and were subjecte...
Source: Comparative Clinical Pathology - August 10, 2022 Category: Pathology Source Type: research

Acute toxicity study of Guiera senegalensis J.F. Gmel methanolic leaf extract in Wistar albino rats through oral administration
AbstractThe abundance of medicinal plants does not guaranteed that they are  safe, as there is dearth of data on their toxicity and safety. The present study evaluated the oral acute toxicity of methanolic leaf extract ofGuiera senegalensis J.F. Gmel (GS), a plant used traditionally for the treatment of epilepsy. In phase one, 12 rats were divided in to 4 groups (n = 3) and orally administered with water, 10 mg/kg, 100 mg/kg, and 1000 mg/kg of GS respectively. In phase two, four rats were divided into four groups (n = 1) and orally administered with water, 1600 mg/kg, 2900 mg/kg, and 5000 mg/kg of GS extract...
Source: Comparative Clinical Pathology - August 10, 2022 Category: Pathology Source Type: research

Significance of LINC00641 and miR-378 as a potential biomarker for colorectal cancer
AbstractIn colorectal cancer (CRC), dysregulation of noncoding RNA expression is a distinguishing factor. Owing to the conflicting results and the insufficient studies on serum microRNA-378 (miR-378) and long intragenic noncoding RNA00641 (LINC00641) expression patterns, we aimed to explore their expression profiles and diagnostic ability in colorectal cancer.  Blood samples were collected from 30 healthy controls and 70 CRC patients. miR-378 and LINC00641 expression levels were determined using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), carcinoembryonic antigen (CEA) levels assessed...
Source: Comparative Clinical Pathology - August 9, 2022 Category: Pathology Source Type: research

TEG assay to assess the altered lipid profile in patients with type 2 diabetes mellitus
This study aimed to investigate the effect of lipid profile in patients with type 2 DM toward hemostasis profile using TEG assay. A cross-sectional study was conducted on patients with type 2 DM in the Endocrinology Outpatient Clinic of Saiful Anwar Hospital from November 2020 to April 2021. We measured the serum level of total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides (TG) using an automatic analyzer. TEG is a diagnostic method to identify hemostatic profiles, including primary, secondary, and tertiary hemostasis. This study used at-test and correlation-regression t...
Source: Comparative Clinical Pathology - August 8, 2022 Category: Pathology Source Type: research