Fish oil protects rat erythrocytes against oxidative stress caused by Trypanosoma evansi infection
AbstractThe effect of omega-3 polyunsaturated fatty acids has been the subject of intense study in many diseases; meanwhile, our understanding regarding the underlying mechanisms of omega-3 fatty acids againstTrypanosoma evansi infection is still limited. A common feature ofT. evansi infection is anemia. Erythrophagocytosis occurs due to direct traumatic actions of protozoa on the red blood cells, but the exact mechanisms of anemia are not yet fully understood. During infection, there is an increase in reactive oxygen species; some are highly toxic and rapidly detoxified by various cellular enzymatic and nonenzymatic mecha...
Source: Comparative Clinical Pathology - February 18, 2017 Category: Pathology Source Type: research

Blood chemistry and haematology of West African Dwarf goats fed Moringa oleifera leaf meal (MOLM) in their diet
AbstractWest African Dwarf (WAD) goat is one of the most prolific goat species in Nigeria, and its production fulfils important socio-economic functions. In Nigeria, high cost of feed and inadequate nutrient intake undermines this goat breed in expressing their full potential. Therefore, the effect of 90  days of feeding MOLM diets on blood chemistry and haematology of WAD goats were investigated. Thirty six, 10–11-months old WAD goats were divided into four groups of nine , and each group was replicated thrice. Each group was assigned to one of the experimental diets in a completely randomized d esign tagged A ...
Source: Comparative Clinical Pathology - February 17, 2017 Category: Pathology Source Type: research

The effect of castration on cortisol changes and blood glucose in horses
In this study, cortisol concentration was measured after a surgery in order to determine the relationship between horse ’s castration, adrenal gland excretion, and level of stress. Twelve horses were selected for surgical castration. Scrotal incision and closed approach technique were performed for both testicles. Blood was taken before surgery, 10 and 30 days after surgery (castration). Blood glucose was measured by glucose oxidase and then serum and plasma hormones cortisol were measured. Finally, Paired samplet test was performed for statistical analysis using SPSS 16 software (SPSS Inc., Chicago, IL, USA). N...
Source: Comparative Clinical Pathology - February 16, 2017 Category: Pathology Source Type: research

Evaluation of wound healing activity of St. John ’s Wort ( Hypericum perfoliatum ) in horses
This study has certainly given a scientific point of view to a century of empiricism about the use of SJW in veterinary practice. The findings of this study seem to suggest that SJW may be considered an “all-in-one” remedy for wound healing, in order to make the management of skin injuries easier even for severe and complicated lesions. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 15, 2017 Category: Pathology Source Type: research

Blood chemistry and haematology of finisher broilers fed with plantain ( Musa paradisiaca L) peel in their diets
AbstractA 4-week study was carried out to determine the effects of partial replacement of maize in broiler finisher diets with plantain peel on their blood chemistry and haematology. One hundred and twenty-six (126), 4-week-old Marshall Broilers weighing 1.0  kg were divided into 3 groups of 42 birds. Each group was replicated 3 times with 14 broiler chicks per replicate and each replicate housed in a pen measuring 1.5 m × 2.0 m. Each group was assigned to one experimental diet in a completely randomized design designated T1 (0%), T2 (10%), and T3 (20%). The condition of housing and managemen...
Source: Comparative Clinical Pathology - February 13, 2017 Category: Pathology Source Type: research

A comparative study on selected APP, alkaline phosphatase and lactate dehydrogenase activities in buffalo and cow with subclinical mastitis
AbstractThe aim of this study was to evaluate the comparative changes of selected acute-phase proteins (APPs), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activities in buffalos and cows with subclinical mastitis. For this purpose, a total of 139 buffalos (Bubalus bubalis) and 146 cows (Holstein or Friesian) from Dakahlia governorate in Egypt were studied. Seventy-six cows and 49 buffalos exhibited subclinical mastitis with positive California mastitis test (CMT) and no clinical signs of mastitis. Results revealed that the most dominant isolated bacteria wereStaphylococcus spp. in both buffalo and cow fello...
Source: Comparative Clinical Pathology - February 13, 2017 Category: Pathology Source Type: research

Effect of salbutamol against the experimental acute colitis in rat model
In conclusion, salbutamol can ameliorate acute ulcerative colitis, possibly via the stimulation of beta2 receptors. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 11, 2017 Category: Pathology Source Type: research

Prevalence and economic importance of Coenurus Cerebralis in sheep and goats in and around “Legahida” district of bale zone, south eastern Ethiopia
AbstractCoenurosis is a disease caused byCoenurus cerebralis, the larval stage ofTaenia multiceps, particularly affecting sheep and goats.T. serialis andT. brauni could also cause coenurosis. The final hosts are domestic and wild canidae. Cross sectional study was conducted from November 2014 to March 2015 to estimate the prevalence and associated risk factors ofCoenurus cerebralis in both sheep and goats in Legahida backyard slabs. The estimation of its economic burden and assessment of knowledge, attitude, and practices (KAP) of the pastoral and agro-pastoral communities were also performed for which questionnaire survey...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Cholinesterase ’s activities in cows supplemented with selenium, copper, phosphorus, potassium, and magnesium intramuscularly during the transition period
AbstractThe cholinesterases are enzymes involved in severe biological functions in mammals; however, it was not yet study in dairy cows during the transition period. In this period, the animals have high energetic demands for milk production, and the supplementation with mineral can be considered an important approach to fulfill this demand. Thus, the aim of this study was to evaluate the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in dairy cows supplemented with minerals based on selenium, copper, phosphorus, potassium, and magnesium intramuscularly in the transition period. The mineral supp...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Morphological features of glands in the gastrointestinal tract of the African pied crow ( Corvus albus )
In conclusion, the data presented in this study represent morphofunctional modifications of the gut of the crow that may be related to their feeding habit, exposure to microorganisms, and species adaptations which could guide the management of this wild species. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Serum biochemical changes following constant rate infusion of Ketoprofen and Ketoprofen + Tramadol combination in laparotomized dogs
AbstractThere is no data on safety of ketoprofen and ketoprofen  + tramadol combination by constant rate infusion for the management of laparotomy-induced pain. The study investigated the serum biochemical changes associated with the use of ketoprofen and ketoprofen + tramadol combination by constant rate infusion in laparotomized dogs. Fifteen adult female Nigerian mongrels were randomly assigned into three groups (n = 5). Group 1 was treated with normal saline; group 2 received 1.0 mg/kg/h K while group 3 received KT at 0.5 mg/kg/h each. Ventral midline was performed on all do...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Toxicity and nutritional studies on Mucuna pruriens leaves from Nsukka, South-Eastern Nigeria
AbstractNutrient, proximate and phytochemical analyses ofMucuna pruriens leaves were done. Acute and sub-acute toxicity studies on the methanol extract were done. Eighteen rats dosed with the extract at 10, 100, 1000, 1600, 2900 and 5000  mg/kg body weights were used for acute toxicity test. For the sub-acute toxicity study, 24 rats randomly allotted into four groups (A–D) of six animals each were used. Graded doses (100, 200, 400 mg/kg) of the extract were administered orally to groups A, B and C, respectively, for 28 days. Gr oup D was the untreated control. Body weight changes, gross and histopathol...
Source: Comparative Clinical Pathology - February 6, 2017 Category: Pathology Source Type: research

Cytotoxic activity of fumonisin B 1 in Vero cells: comparison between 2D and 3D structural microplates
AbstractThe present study aimed to estimate cytotoxic activity of fumonisin B1 (FB1) in Vero cells using crystal violet staining assay (CVA) and lactate dehydrogenase assay (LDH) performed in flattened (2D) and three dimensional (3D) bottom (V, U, and M) microplates. Vero cells were exposed to different concentrations of FB1 ranging from 0 to 10  μg/ml for 24 and 48 h. Tween 20 was used as a positive control and acetonitrile 50% in water was used as a negative control. Results revealed that CVA could be performed in the F type microplate for 24 and 48 h but it was useless in the 3D microplates. LDH assay ...
Source: Comparative Clinical Pathology - February 4, 2017 Category: Pathology Source Type: research

Subchronic toxicity studies of aqueous leaf and stem bark extract of Irvingia gabonensis in male albino wistar rats
This study investigated the effect of aqueous leaf and stem bark extracts ofI. gabonensis on the haematology, pathomorphology and some clinical chemistry profiles of male albino wistar rats following subchronic exposure. The extract was administered using the oral route. Group A served as control and was given only distilled water. Groups B, C and D were treated with the extract at 100, 1000 and 2000  mg/kg, respectively. The parameters assessed to determine the effect of subchronic administration of the extract were: packed cell volume (PCV), haemoglobin concentration (Hb), total red blood cell count (TRBC), total wh...
Source: Comparative Clinical Pathology - February 3, 2017 Category: Pathology Source Type: research

Examining responses of chicken embryonic neural stem cell to infectious laryngotracheitis virus infections
AbstractInfectious laryngotracheitis virus (ILTV) infection causes economic losses in the poultry industry. Outbreaks of the infection in commercial poultry are controlled by using attenuated live vaccines. The genomic and antigenic characteristics between virulent and vaccine strains of ILTV are very similar, thus the differential diagnosis of ILTV strains is targeted in controlling programs. Here, the chicken embryonic neural stem (cENS) cell serves as a host model to study virulence characteristics of ILTVs. The susceptibility of cENS cells to different ILTV strains infection was investigated by analyzing the impact of ...
Source: Comparative Clinical Pathology - January 27, 2017 Category: Pathology Source Type: research

Genetic influence of DNAM-1 (DNAX accessory molecule-1) gene polymorphism (rs 763361) on susceptibility and disease activity of systemic lupus erythematosus and rheumatoid arthritis in Egyptian patients
This study aimed to determine the possible genetic influence of SNP on disease activity in SLE (represented by SLEDAI score) and in RA patients (represented by DAS28). Fifty SLE patients compared to 43 healthy controls and 35 RA patients compared to 30 healthy controls were included in the study. The PCR-RFLP technique was used to detect DNAM-1(C/T rs 763361) gene polymorphism. Analysis of the frequency distribution of both allele and genotype frequency distribution of SLE and RA patients compared to healthy control subjects did not reveal a significant association between SLE and RA patients and the rs763361. Analysis of ...
Source: Comparative Clinical Pathology - January 26, 2017 Category: Pathology Source Type: research

Use of homeopathic product to prevent ketosis in the dairy sheep during the transition period
AbstractPregnancy toxemia is a severe problem in sheep in the transition period, which is most likely related to feeding. There is a need to seek alternatives to this problem, and the homeopathic industry has invested in products for this purpose. The product (Figotonus ®) tested in this work was added to the diet of dairy sheep at different dosages (1, 5, and 10 g/day/animal) to verify the effects on animal health indicators, focusing mainly on the reduction of cases of ketosis. In all, 32 Lacaune ewes were used, with confirmed pregnancy presenting two to six bi rths during their lifetime. The following blood bio...
Source: Comparative Clinical Pathology - January 25, 2017 Category: Pathology Source Type: research

Evaluation of salivary level of IL-10 in patients with oral lichen planus, a preliminary investigation
AbstractOral lichen planus is an inflammatory disorder with T-cell-mediated immunological etiology. The aim of this study was to evaluate the IL-10 in saliva of OLP patients and compare it with healthy people. In this cross-sectional study, 44 patients with oral lichen planus and 44 healthy people were enrolled to the study. Five to 10  ml un-stimulated whole saliva samples were collected in the morning, and concentration of IL-10 was measured using enzyme-linked immunosorbent assay (ELISA). The mean of salivary IL-10 was more in the patient group (0.2 ± 0.92) than the healthy group (0.1 ±...
Source: Comparative Clinical Pathology - January 25, 2017 Category: Pathology Source Type: research

Histopathological study in wild freshwater stingrays Potamotrygon motoro in the Peruvian Amazon
The objective of the present study was to investigate the occurrence of histopathological alterations in some tissues of the Amazonian freshwater stingrayPotamotrygon motoro. Thirty-one specimens were collected from the Amazon basin and transported to a commercial aquarium in district of Juan Bautista, province of Maynas, department of Loreto, Peru. The histopathological study showed monogeneans in gills (2/31),Piscinoodinium pillulare in gills and skin (2/31), digenean metacercariae in liver (3/31), and coccidia in the lumen and mucosa of the spiral valve (2/31). In addition, there were found non-infectious lesions in the...
Source: Comparative Clinical Pathology - January 24, 2017 Category: Pathology Source Type: research

Immunohistochemical expression of transforming growth factor Beta-1 in canine mammary carcinomas: its relationships with histologic grading, survival rate, and recurrence
In this study we aimed to investigate Transforming Growth Factor Beta-1 (TGF- β1) expression in canine mammary carcinomas (CMCs) by immunohistochemistry and its relationships with histologic grading, survival rate, and recurrence. Tissues were selected from 35 cases of CMCs and were included in a 2-year follow-up study. Immunohistochemistry for detection of TGF-β1 protein w as carried out using Avidin-Biotin Complex (ABC) method, and the immunostained slides were scored according to the distribution and intensity of positive reactions. Results indicated that TGF β-1 expression increased significantly in CMCs...
Source: Comparative Clinical Pathology - January 21, 2017 Category: Pathology Source Type: research

Two foals with ectrodactyly in hindlimb: first report
AbstractEctrodactyly refers to the congenital absence of phalanges as total or partial. In the veterinary literature, ectrodactyly has been described in dogs and domestic cats as a rare congenital heterogeneous disorder. The current report has described unilateral ectrodactyly in the left hindlimb of two born foals in an area with presence of Iranit and zinc manufactures. One of the foals was lack of the third phalanx and the other one had not the third and second phalanxes. No other abnormality was found in these cases. Various teratogens may be responsible for occurring skeletal anomalies. To the best of our knowledge, t...
Source: Comparative Clinical Pathology - January 21, 2017 Category: Pathology Source Type: research

A comparative analysis of the molecular basis of fibrosis between tissues
This article discusses key molecular mechanisms of fibrosis within tissues and highlights the similarities and differences amongst key pathways. There is compelling evidence for the involvement of toll-like receptor-4 and transforming growth factor β within fibrosis throughoutall tissue types. With regards to other molecules, although there are similarities between tissues, evidence is inconsistent. In order to gain therapeutic advances in the prevention or treatment of fibrosis, research should strive to understand specific molecular mechanisms in models that closely replicate human disease. Further, there should be ...
Source: Comparative Clinical Pathology - January 20, 2017 Category: Pathology Source Type: research

Erratum to: Evaluation of the healing effect of honey and colostrum in treatment of cutaneous wound in rat
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 20, 2017 Category: Pathology Source Type: research

Pathological and biochemical studies on enterotoxemia in sheep
In conclusion, enterotoxemia as a fatal enteric disease is associated with some characteristic clinical, biochemical, bacteriological, and histopathological alterations. These alterations can be a useful aid for rapid diagnosis of enterotoxemia in sheep farms. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 19, 2017 Category: Pathology Source Type: research

Overexpression of the giant FAT1 cadherin gene and its prognostic significance in de novo acute leukaemia patients
AbstractFAT1 is a type 1 transmembrane protein belonging to the cadherin superfamily. Many reports showed either overexpression of FAT1 gene or its loss in solid cancers. Limited studies evaluated its importance in leukemogenesis. Real-time quantitative reverse transcriptase polymerase chain reaction (RTQ-PCR) was used to examine the levels of FAT1 mRNA expression in 50 paediatric acute lymphoblastic leukaemia (ALL) and 50 adult cytogenetically normal acute myeloid leukaemia (CN-AML). Each group was compared with 20 age- and gender-matched healthy controls. Peripheral blood (PB) samples were collected at time of diagnosis ...
Source: Comparative Clinical Pathology - January 17, 2017 Category: Pathology Source Type: research

Anti-diabetic activity of Garcinia xanthochymus seeds
AbstractIn the present study, extracts ofGarcinia xanthochymus seeds were used to determine anti-diabetic, anti-oxidant, and biochemical activities in alloxan-induced diabetic mice. Of the petroleum ether (G. xanthochymus petroleum ether extract (GXSPE)) and methanol (G. xanthochymus methanol extract (GXSME)) seed extracts, 100 and 200  mg/kg were used and compared with normal, diabetic, and glibenclamide groups for various activities. The parameters like blood glucose, body weight, serum biochemicals, serum enzymes, and anti-oxidative enzymes in different groups were monitored at regular intervals. The extracts produ...
Source: Comparative Clinical Pathology - January 16, 2017 Category: Pathology Source Type: research

Strong expression of interleukin-17 is associated with higher histologic grades in canine mammary carcinoma
This study investigates interleukin-17 expression in canine mammary tumours by immunohistochemistry and its relationships with histologic grades, tumour recurrence and survival after 2  years. Results showed that 23 samples (63.88%) showed strong immunoreactivity for interleukin-17 and 13 samples (36.11%) showed moderate immunoreactivity. Interleukin-17 expression is significantly increased in canine mammary tumours compared to normal canine mammary tissues (P 
Source: Comparative Clinical Pathology - January 16, 2017 Category: Pathology Source Type: research

Silymarin and methionine application on treatment of liver chronic diseases by aflatoxicosis in rabbit ( Oryctolagus cuniculi ) —case report
AbstractThis case report describes the treatment of chronic liver disease in rabbit (Oryctolagus cuniculi) caused by consumption of feed containing mycotoxin and later treated with silymarin. A young female rabbit was referred to veterinary care with a history of apathy, appetite loss, and difficulty breathing without a clear medical history. The physical examination revealed an increase in the abdominal volume, an observation confirmed by radiographic analysis showing the presence of ascites. Through the serum biochemistry evaluation, it was an evidenced liver disease. After 1  month of treatment, the animal did not ...
Source: Comparative Clinical Pathology - January 13, 2017 Category: Pathology Source Type: research

Farm-level evaluation of enrofloxacin resistance in Escherichia coli isolated from broiler chickens during a rearing period
In this study, enrofloxacin resistance was investigated in commensalEscherichia coli isolated from broiler chickens using phenotypic and genotypic assays. FiveE. coli isolates were recovered from the cloacal swabs of 15 broiler chickens in the farms at the ages of 1  day (stage 1), 30 days (stage 2), and 1 day before slaughter (stage 3). Minimum inhibitory concentration results for enrofloxacin showed that the prevalence of resistance significantly increased (P 
Source: Comparative Clinical Pathology - January 12, 2017 Category: Pathology Source Type: research

Stability of some clinical biochemistry parameters in equine serum/plasma stored at refrigerator and room temperatures: a preliminary study
AbstractAccurate measurement of clinical biochemistry parameters is an essential tool for the correct interpretation of clinical biochemistry abnormalities. A general problem faced by most clinical laboratories is inability to process blood samples received late or submitted in large numbers. This delay in sample processing is usually unavoidable. This present study evaluated the stability of some biochemistry parameters in equine blood before and after storage at room (25  °C) and refrigerator (4 °C) temperatures. Five milliliters of blood were collected from external jugular veins of 17 apparently healt...
Source: Comparative Clinical Pathology - January 10, 2017 Category: Pathology Source Type: research

Influence of dexamethasone-induced stress on oxidative stress biomarkers in non-pregnant does experimentally infected with Brucella melitensis
This study aimed at evaluating biomarkers of oxidative stress in dexamethasone-treated non-pregnant Boer goats experimentally infected withB. melitensis. Eighteen healthy, non-pregnant goats were divided into three groups: A, B, and C of six animals each. Group A was treated with dexamethasone (2  mg/kg) for 7 days prior to inoculation with 107 CFU ofB. melitensis ocularly. Group B was not treated but inoculated with similar dose ofB. melitensis as in group A above while group C was administered normal saline. Blood samples were collected periodically through day 42  post inoculation (pi). Plasma cortis...
Source: Comparative Clinical Pathology - January 7, 2017 Category: Pathology Source Type: research

Evaluation and comparison of a set of oxidative and antioxidative biomarkers in cystinuric patients with age- and sex-matched healthy subjects
AbstractThe present study evaluates and compares the serum biomarkers of oxidative stress —superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), malondialdehyde (MDA), glutathione reductase (GR), and total antioxidant capacity (T-AOC), in a case-control design study of 30 diagnosed patients with cystine stone and 30 sex- and age-matched healthy control group. The levels of SOD were significantly lower in patients (14.47 ± 15.13 ng/mL) when compared with control group (26.18 ± 15.34 ng/mL),p value 0.003. Similarly, the levels of iNOS were significantly lower in p...
Source: Comparative Clinical Pathology - January 6, 2017 Category: Pathology Source Type: research

Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction)
AbstractEthanolic extract of bitter fraction ofStevia rebaudiana (Srbf) was extracted to investigate its antihyperglycemic and protective effects on renal structural changes in STZ-induced diabetes. Thirty-five male mice were divided into five groups randomly; the first group as non-diabetic control, the second group as untreated diabetic, the third group treated with glibenclamide 0.5  mg/kg, and the fourth and fifth groups treated withSrbf by 200 and 400  μg/kg bw through gavage, respectively, for 15 days. Diabetes was induced in the second to fifth groups by administration of 60 mg/kg bw of strept...
Source: Comparative Clinical Pathology - January 3, 2017 Category: Pathology Source Type: research

Histopathological assessment of chronic Corynebacterium pseudotuberculosis infection in the reproductive tract and iliac lymph node of Katjang does
In this study, 18 non-pregnant healthy Katjang does aged 2  years old were randomly divided into two groups of nine goats each. The first group were all experimentally inoculated with 1 ml of 107 cfu of liveC. pseudotuberculosis through intradermal route while the second group was given 1  ml PBS (pH 7) intradermally. Three animals from each group were withdrawn and culled after 30, 60 and 90 days post infection and the reproductive organs (vagina, cervix, uterus, uterine horns, and ovaries) and iliac lymph nodes were collected in 10 % buffered formalin, processed, sectioned, and stained ...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Prevalence and intensity of parasitic infection in domestic ducks ( Anas platyrhynchas ) in Gilan Province, Northern Iran
This study was carried out to assess the levels of parasitic infection in domestic ducks (Anas platyrhynchas) in Gilan Province, Northern Iran. During the summer of 2015, samples were collected randomly from local markets at different villages in Gilan Province. Blood samples were collected in EDTA-treated tubes, blood smear slides created, stained with giemsa and examined for the presence of blood protozoa. Ectoparasites samples were collected and identified according to Wall and Shearer (2001). To assess endoparasitic infection, the ducks ’ gastrointestinal tracts were removed and the various parts identified and o...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Occurrence of gastrointestinal helminths in horses and risk factors for infection
This study aimed to monitor the occurrence of gastrointestinal parasites in horses evaluating different rural properties in Santa Catarina State, Southern Brazil. Fresh samples of feces were collected and processed by the McMaster technique, quantifying the number of eggs per gram of feces (EPG), followed by coproculture for identification of helminths. The population investigated was composed of 111 horses of varying ages (1 –27 years, 63 males and 48 females). Among all the animals evaluated, 76 animals showed EPG ≥50, representing 63.96 % (IC95  % 54.24 –72.70) of samples positive for gastr...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Butyrylcholinesterase activity in dairy cows naturally infected by Dictyocaulus viviparous and treated with eprinomectin
AbstractThe aim of this study was to evaluate the activity of butyrylcholinesterase (BChE) in dairy cows naturally infected byDictyocaulus viviparus and treated with eprinomectin. Among 22 serum samples, 10 were of animals with mild clinical disease and 12 with severe clinical disease. The samples were collect on days 0 (day of diagnosis) and 10 post-treatment (a single dose of eprinomectin [360  μg/kg, topically]). We evaluated the activity of BChE, alanine aminotransferase (ALT), aminotransferase andaspartate (AST), and gamma-glutamyl transferase (GGT) in serum. There was no significant difference between groups ...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Visceral and serum lysozyme activities in some freshwater fish (three catfish and two carps)
AbstractThe lysozyme activities were investigated in three catfish: hybrid catfish [Clarias macrocephalus (G ünther 1864) xClarias gariepinus (Burchell 1822)],Hemibagrus filamentus (Fang and Chaux 1949), andKryptopterus apogon (Bleeker 1851) and two carps:Oreochromis niloticus (Linnaeus 1758) andChanna striata (Bloch 1793). Fish samples used in this study were from two sites of Chiang Mai province;C. macrocephalus xC. gariepinus andO. niloticus were from Ton Payom Market, Amphoe Muang.H. filamentus,K. apogon, andC. striata were from Doi Tao Lake, Amphoe Doi Tao. The serum, brain, liver, and kidney of the catfish and c...
Source: Comparative Clinical Pathology - January 1, 2017 Category: Pathology Source Type: research

Effects of glutamine and glutamate supplementation in dogs with hemorrhagic enteritis
This study evaluated the effect of glutamine and glutamate supplementation on hematological and biochemical biomarkers in dogs with clinical enteritis. Fifteen young dogs (3 –10 months) with clinical enteritis were divided into two groups: group 1 (G-CON)—five animals subjected to medical treatment without supplementation and group 2 (G-GLN)—ten animals subjected to medical treatment and orally supplemented with 0.5 g/Kg per day of a glutamine and glutamate for 14 days. The following variables were measured: blood count, plasma glutamine and glutamate, total plasma protein (TPP), albumin, g...
Source: Comparative Clinical Pathology - December 31, 2016 Category: Pathology Source Type: research

Flow cytometry analysis of lymphocyte subsets in bronchoalveolar lavage: comparison between lung non-Hodgkin lymphomas and reactive diseases
AbstractDeepened flow cytometry (FCM) analysis of bronchoalveolar lavage fluid (BALF) cells can disclose clonal B and abnormal T lymphocytes in case of lung involvement in non Hodgkin lymphoma (NHL). The possible role of routine FCM BALF analysis in diagnosing NHL involving the lungs is largely undetermined. To evaluate whether differences exist, within FCM screening of lymphocyte subsets, between BALFs from lung NHL and BALFs from reactive diseases. We compared alveolar leukocyte and lymphocyte data obtained using flow cytometry in 17 lung NHL cases with the median corresponding data detected in 208 controls, matched with...
Source: Comparative Clinical Pathology - December 28, 2016 Category: Pathology Source Type: research

Differentiation of populations with different fluorescence intensities with a machine-learning based classifier
AbstractAs proven fast and reproducible, cellular diagnostics based on machine learning has gained importance lately by its capability to predict different classes of cells based on cell and nuclear morphology, replacing specific markers. The present study aimed to verify if it is possible to differentiate objects with different fluorescence intensities using a machine learning based classifier. Samples were prepared with one drop of fluorescent mounting medium and bead solution (cytocal). Images were generated in .tif format using iCys image cytometer and saved for all relevant channels of fluorescence. Two softwares were...
Source: Comparative Clinical Pathology - December 27, 2016 Category: Pathology Source Type: research

Effects of aqueous and dimethyl sulfoxide extracts of Peganum harmala on juveniles of Dictyocaulus filaria
AbstractThe aim of the present study was to investigate the larvicidal effect of aqueous and dimethyl sulfoxide extracts ofPeganum harmala on larvae ofDictyocaulus filaria. Seed extract was prepared, and serial dilutions of 200, 100, 50, 25, 12.5, 6.25, and 3.12  mg/ml were prepared using distilled water and dimethyl sulfoxide (DMSO). Two genders ofD.filaria were collected from sheep lung and cultured withEscherichia coli bacteria as parasites nutrition source. Ten juveniles were placed in deep slide to assess the larvicidal effects based on disability in the movement, degranulation, and appearance of the wrinkles on ...
Source: Comparative Clinical Pathology - December 26, 2016 Category: Pathology Source Type: research

Assessment of phage cocktails with extended host range activity against antibiotic resistant strains of Pseudomonas aeruginosa
AbstractThe appearance of antibiotic resistant strains ofPseudomonas aeruginosa especially pan-drug resistant (PDR) strains has caused efforts to identify alternative antibacterial methods such as phage therapy. The purposes of this study were the isolation and preparing of effective phage cocktails against antibiotic resistant strains ofP.aeruginosa. Determination of acquired resistance profiles of these isolates was performed according to new definition of CDC and European Centre for Disease Prevention and Control as multidrug-resistant (MDR), extended-drug resistant (XDR) and PDR, and data were analyzed using WHONET5.6 ...
Source: Comparative Clinical Pathology - December 21, 2016 Category: Pathology Source Type: research

Environmental and host factors affecting seroprevalence of bluetongue virus infections of sheep
In this study, the result of testing of 928 samples from a serologic survey of sheep with BTV infections in the Chaharmahal-Va-Bakhtiari province of Iran were analyzed for association between environmental (climate) and host (age, sex, abortion history) factors. Bivariate analysis indicated that environmental and two host factors were associated with the test result. After the chi-square test, a higher seropositive was observed in the plain region (59.94%), compared with the mountain region (24.24%) (df  = 2, χ2 = 104.59,P 
Source: Comparative Clinical Pathology - December 20, 2016 Category: Pathology Source Type: research

Simultaneous application of human amniotic membrane and Tachosil ® in the repair of recto-vaginal fistula in an animal model
AbstractPatients with recto-vaginal fistula (RVF) suffer from distressing symptoms like the passage of stool and gas through the vagina. Although surgeons employed various techniques to address this disorder, however, current techniques are invasive and their success rate is variable. Considering the notable features of human amniotic membrane (HAM) as a bio-prosthesis and Tachosil ® as a type of biologic mesh, the present study was designed to assess the effect of HAM in the presence of Tachosil® on the repair of RVF in a rabbit model. Twelve New Zealand rabbits weighing 2–2.5 kg with the age of 6&ndash...
Source: Comparative Clinical Pathology - December 20, 2016 Category: Pathology Source Type: research

Antioxidant and antileukemic activity of chemical components from bark of Mangifera casturi
In this study, antioxidant and antileukemic activity of chemical components from bark ofMangifera casturi was investigated. Research findings have shown that several extracts ofM. casturi have potent bioactivity. The methanol extract ofM. casturi bark was partitioned successively to yieldn-hexane fraction (6.7%), ethyl acetate (EtOAc) fraction (24.1%), andn-butanol (n-BuOH) fraction (28.1%). Five compounds were isolated from EtOAc fraction and one compound was isolated fromn-hexane fraction. These compounds were identified as methyl gallate (1), taxifolin (2), pyrocatechuic acid (3), gallic acid (4), glucogallin (5), and &...
Source: Comparative Clinical Pathology - December 20, 2016 Category: Pathology Source Type: research

Enrofloxacin stimulates cell death in several tissues of vannamei shrimp ( Litopenaeus vannamei )
AbstractThe aim of this research was to investigate the effect of enrofloxacin on vannamei shrimp (Litopenaeus vanammei) by measuring its accumulation and withdrawal time, in addition to its histopathological effects. The effect of enrofloxacin on the cells of vannamei shrimp has not yet been reported. The enrofloxacin levels were analysed by enzyme-linked immunosorbent assay (ELISA), and several tissues and organs, including the hepatopancreas, gills and muscles, were histopathologically analysed. Regarding the withdrawal time from shrimp tissue, the optimum enrofloxacin dose is 26.06  ± 13.67 μg/...
Source: Comparative Clinical Pathology - December 19, 2016 Category: Pathology Source Type: research

Detection of Clostridium perfringens bacterium in the clinical specimens from blackleg calves: a report of two cases
AbstractThis paper describes the first report involving two cases of blackleg caused byClostridium perfringens Type D as a main causative agent with an emphasis on clinical symptoms, the bacteriological and pathological analyses, and molecular detection of the pathogens by PCR method. Two calves of a flock were presented to the veterinary hospital of Shahid Bahonar University of Kerman, Iran. Both calves died suddenly and showed considerable muscle crepitation in the posterior limb. Severe necrosis of thigh muscles in both hind legs, which had caused dark discoloration of tissue with metallic sheen, large amount of thin sa...
Source: Comparative Clinical Pathology - December 16, 2016 Category: Pathology Source Type: research

Safety assessment of aqueous acetone extract of Clerodendrum infortunatum L. in Wistar rats
AbstractClerodendrum infortunatum L. is a traditional medicinal plant used for the treatment of bronchitis, malarial fever, benign tumour etc. Antioxidant screening revealed that the aqueous acetone extract exhibited the greatest antioxidant activity. In the present study, toxicity analysis of the aqueous acetone extract was carried out in male Wistar rats to evaluate its impact on the liver and serum biochemical parameters. Male Wistar rats were divided into six groups and groups II to VI were treated with a single dose of 250, 500, 1000 and 2000  mg/kg body weight of aqueous acetone extract and silymarin, respective...
Source: Comparative Clinical Pathology - December 16, 2016 Category: Pathology Source Type: research

The diagnostic accuracy of oxidative stress biomarkers in sheep with pneumonic pasteurellosis
AbstractThe goal of this study was to decide the diagnostic accuracy of oxidative stress biomarkers in infected sheep withPasteurella multocida. Blood samples were collected from 56 sheep (36 naturally infected withPasteurella multocida and 20 healthy controls) belonging to one farm in Al-Ahsa region, Saudi Arabia. Serum samples were evaluated for oxidative stress markers (malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT)). Additionally, nasal swabs and bronchoalveolar lavages were gathered from all sheep for bacteriological investigations. Receiver operating characteristic curve was u...
Source: Comparative Clinical Pathology - December 15, 2016 Category: Pathology Source Type: research