Comparative assessment of antibiotic potency loss with time and its impact on antibiotic resistance
AbstractA wide range of diverse antibiotics is available in the current world ’s drug market, which is mostly being used for the treatment of infectious diseases in humans as well as in veterinary. A large number of new antibiotics are continuously being added to the existing list. However, at the same time, most of the pathogens have tended to overcome antibiotic stress du e to their specific mutations and adaptations with time. Hence, several antibiotics turn to be non-effective in the long run. Moreover, antibiotics also lose their effectiveness with time as they approach their expiry period. The goal of this stud...
Source: Comparative Clinical Pathology - October 19, 2016 Category: Pathology Source Type: research

Claudin-17 reduced expression is associated with high histologic grade and shorter overall survival in canine mammary carcinoma
AbstractThe present study investigates Claudin-17 expression in canine mammary carcinomas by means of immunohistochemistry and its relationships with histologic grades, overall survival and tumour recurrence. Our results show that Claudin-17 expression is reduced significantly in canine mammary carcinomas compared with normal canine mammary tissues. High histologic grade and shorter overall survival have been shown to be significantly associated with Claudin-17 reduced expression. Results of this study suggest a possible relationship between reduction of Claudin-17 and canine mammary carcinoma pathogenesis. (Source: Compar...
Source: Comparative Clinical Pathology - October 16, 2016 Category: Pathology Source Type: research

Gastroprotective potential of melatonin versus melatonin loaded niosomes on gastric ulcer healing in rats
AbstractThe present study was conducted to investigate the therapeutic effects of melatonin and melatonin-loaded niosomes on ethanol-induced gastric lesions. Morphological and biochemical changes associated with each drug were investigated. Seventy apparently healthy female albino rats, weighting about 200 –250 g were randomly assigned to 4 experimental groups. The first group (induction group) included 10 rats that were given 1 ml 70 % ethanol and sacrificed after 1 and 1/2 hour. The second group (melatonin-treated group) included 25 rats that were administrated with 2 mg/rat melatonin by gastr i...
Source: Comparative Clinical Pathology - October 13, 2016 Category: Pathology Source Type: research

Effects of dietary supplementation of Lactobacillus Plantarum on survival, hematological profile and histopathology of selected organs of Clarias gariepinus fingerlings
This study evaluates the effects of dietary supplementationof Lactobacillus plantarum (LP) on survival of hematological profile and histopathology of selected organsof Clarias gariepinus fingerlings. TenClarias gariepinus fingerlings were allocated to five treatments, with three replicated subgroups each, fed and reared for 12  weeks.LP isolated from corn slurry was cultured using standard procedures. Five iso-nitrogenous diets were formulated at 35  % crude protein (T0, T1, T2, T3, and T4) withLP at inoculation rate of 0  × 107 (T0), 0.5  × 107 (T1), 1.0  × 107 (T2...
Source: Comparative Clinical Pathology - October 12, 2016 Category: Pathology Source Type: research

Proteomic impacts of electromagnetic fields on the male reproductive system
AbstractThe use of mobile phones and other wireless transmitting devices is increasing dramatically in developing and developed countries, as is the rate of infertility. A number of respected infertility clinics in Australia, India, USA, and Iran are reporting that those who regularly use mobile phones tend to have reduced sperm quantity and quality. Some experimental studies have found that human sperm exposed to electromagnetic fields (EMF), either simulated or from mobile phones, developed biomarkers of impaired structure and function, as well as reduced quantity. These encompass pathological, endocrine, and proteomic c...
Source: Comparative Clinical Pathology - October 12, 2016 Category: Pathology Source Type: research

Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
In this study, 75 Sprague-Dawley rats were divided into 3 groups of 25 rats, which were further subdivided into 5 subgroups each. Group 1 (negative control) was given distilled water, group 2 (positive control (PC)) administered 10  mg/kg of lead acetate (LA) orally/daily, while groups 3 (T1), 4 (T2), and 5 (T3) were given LA 10 mg/kg and graded concentrations (100, 150, and 200 mg/kg) each of NS. One group each, comprising of 25 rats, was euthanized at days 30, 60, and 90 for collection of blood plasma, epididymis, and org an tissues fixed in 10 % buffered formalin at each time interval. The right caud...
Source: Comparative Clinical Pathology - October 11, 2016 Category: Pathology Source Type: research

Evaluation of the healing effect of honey and colostrum in treatment of cutaneous wound in rat
In this study, 80 adult female Sprague Dawley rats were randomly divided into four groups as negative control group, groups II, III, and IV were treated by honey, bovine colostrum, and honey + colostrum, respectively. Rats were anesthetized, hair was removed from the back, and thena wound was made on the back. Visual observation, histopathological examination, and biomechanical study were performed on days 3, 7, 14, and 21 after operation. Wound area in rats which taken colostrum and honey was lower than other groups. Promotion of wound contraction and epithelialization in rats that were treated with honey and colostrum wa...
Source: Comparative Clinical Pathology - October 10, 2016 Category: Pathology Source Type: research

Efficacy of ARACNE algorithm for inferring canine B-cell lymphoma gene regulatory network (GRN)
AbstractLymphoma is the most frequent hematopoietic cancer in dogs. Canine B-cell lymphoma has been proposed as an ideal model of human non-Hodgkin ’s lymphoma (NHL). Critical genes playing important roles in the cancer progression can be detected using the reconstruction and analysis of gene regulatory network (GRN). GRNs are inferred using various computational algorithms, whereARACNE is on the most important and efficient algorithms. Here, we evaluated the efficacy ofARACNE to reconstruct canine B-cell lymphoma GRN via different computational analyses. Hence, the gene expression profile of GSE43664 was downloaded ...
Source: Comparative Clinical Pathology - October 10, 2016 Category: Pathology Source Type: research

Pathological and diagnostic investigations of abortions and neonatal mortality associated with natural infection of Brucella abortus in dromedary camels
This study was carried out to diagnose and investigate the pathological lesions associated withBrucella abortus in the fetuses and placenta of naturally infected dromedary camels. For diagnosis, the blood samples from infected dams were subjected to Rose Bengal Plate Test (RBPT) and blood PCR. The placental and fetal lung tissue samples were subjected to pathological examination and PCR. Nine cases of abortions and one case of early neonatal mortality was reported in the infected camels. The hematological parameters in infected dams revealed anemia and leukocytosis. The important pathological lesions in the infected fetuse...
Source: Comparative Clinical Pathology - October 9, 2016 Category: Pathology Source Type: research

The effect of Portulaca oleracea (purslane) seeds on hemoglobin levels in adolescent girls with iron deficiency anemia: a randomized comparative trial
AbstractIron deficiency anemia (IDA) is the most severe and the latest stage of iron deficiency. Consumption of purslane seeds is a traditional medicine approach for the treatment of anemia. We aimed to compare the effect of purslane seeds and ferrous sulfate (FS) on the hemoglobin (Hb) level of adolescent girls suffering from IDA. In this single-blind randomized comparative clinical trial, the effect of purslane seeds was compared with a FS tablet in 53 adolescent girls. Included subjects were within the age range of 15 –18 years; with Hb levels of 10–12 g/dL and serum ferritin levels lower than 12&n...
Source: Comparative Clinical Pathology - October 9, 2016 Category: Pathology Source Type: research

Chemokine receptor ’s expression in human adipose derived mesenchymal stem cells primed with valproic acid
AbstractAmong stem cells, mesenchymal stem cells (MSCs) are ideal for practical regenerative medicine and among MSCs, adipose-derived (Ad)-MSCs are the best sources for this regard. Migration or homing to the injured tissue is the main property of MSCs which is related to chemokine receptors on their cell membrane and could be affected by some materials such as valproic acid (VPA). The present study investigated whether VPA could increase the expression of these receptors. In doing so, human Ad-MSCs were isolated from adipose tissue (AT), and after primary culture and characterization, the cells in passage four were primed...
Source: Comparative Clinical Pathology - October 7, 2016 Category: Pathology Source Type: research

Molecular investigation of Anaplasma and Ehrlichia natural infections in the dromedary camel ( Camelus dromedarius ) in Iran
This study describes the first molecular prevalence of CandidatusA. camelii in Iranian dromedary camels. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - October 7, 2016 Category: Pathology Source Type: research

Hematological and histopathological assessment of pacu ( Piaractus mesopotamicus ) after treatment of pathogens with veterinary medicinal products
This study performed hematological and histopathological evaluations of pacu (Piaractus mesopotamicus) that had been naturally infected withAeromonas sp.,Streptococcus sp.,Ichthyophthirius multifiliis,Trichodina heterodentata, andAnacanthorus penilabiatus and treated with enrofloxacin and toltrazuril or with florfenicol and thiamethoxam. After 7  days of treatment from nine fishes were collected blood, via caudal puncture, and samples of the gills, liver, and kidneys. Following toltrazuril and enrofloxacin treatment, fish exhibited leukocytosis with lymphocytosis. With thiamethoxam and florfenicol treatment, the fish ...
Source: Comparative Clinical Pathology - October 6, 2016 Category: Pathology Source Type: research

The effect of curcumin (Curcuma longa L.) on circulating levels of adiponectin in patients with metabolic syndrome
AbstractIn modern societies, metabolic syndrome (MetS) is a highly common disease, which is closely associated with the increased risk of cardiovascular disease (CVD). Metabolic syndrome is usually accompanied by low levels of adiponectin, which is a key regulator of insulin sensitivity and reduced inflammation of the tissue. Adiponectin also reduces systemic insulin resistance and predicts cardiovascular disease. Curcumin has several beneficial effects on risk factors of metabolic syndrome. This polyphenol can affect almost all components of metabolic syndrome including insulin resistance, hypertension and obesity. Accord...
Source: Comparative Clinical Pathology - October 6, 2016 Category: Pathology Source Type: research

High platelet count and high probability of CALR detection in myeloproliferative neoplasms
In this study, we used PCR-PAGE and DNA sequencing methods for detection of CALR mutations and analysis of laboratory findings in MPN patients. BCR-ABL1, JAK2V617F, andMPL515 were assessed using RT-PCR and ARMS-PCR methods in 103 suspected MPN patients. CALR was assessed in patients negative for BCR-ABL1 and JAK2V617Fpatients as well as six cell lines (Jurkat, U937, NB4, KCL22, THP1, and K562) using PCR-PAGE and DNA sequencing. Finally, laboratory data of patients were obtained. BCR-ABL1 and JAK2V617F were detected in 39 (37.86  %) and 34 (33 %) MPN patients, respectively. Two patients had combined BCR-ABL1/JAK2V...
Source: Comparative Clinical Pathology - October 3, 2016 Category: Pathology Source Type: research

Erratum to: The effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 27, 2016 Category: Pathology Source Type: research

Fungal flora in the mouth of venomous and non-venomous snakes
This study was conducted to identify potentially pathogenic fungal flora in the oral cavities of venomous and non-venomous snakes. Sterile swabs were used to retrieve samples from the oral cavities of different snakes followed by medium-specific culture. Slide culture technology was also used in the identification of fungal flora.Rhizopus andMucor represented the most common fungal flora each making up 29  % of the total.Alternaria, Cladosporium, Aspergillus andCandida represented a significantly smaller portion of fungal pathogen load at around 3  % each. Fungi, Yeast (19.4 %) and Penicillium (9.7 %), ...
Source: Comparative Clinical Pathology - September 26, 2016 Category: Pathology Source Type: research

CD63-Alix-Rab27a exosome axis is identically influenced in Chediak-Higashi syndrome
AbstractChediak-Higashi syndrome is characterized by congenital abnormality in exocytosis activity, and accumulation of intracellular lysosome-like structures different types of cells within the body. Accordingly, the lack of appropriate exocytosis and deficiency in lysosomal trafficking accounts for immune-related hypoactivity. Exosomes are other types of cell-derived vesicles that play key role in cell-cell cross talk. However, the possible effect ofLYST gene mutation in exosome trafficking and biogenesis remains to be clarified. Herein, we investigated the expression of CD63-Alix-Rab27a exosome axis in Chediak-Higashi s...
Source: Comparative Clinical Pathology - September 26, 2016 Category: Pathology Source Type: research

The Semelil (ANGIPARS ™) ameliorates cardiac functional disturbances after experimental chronic myocardial infarction model in rabbit
AbstractMyocardial infarction (MI) is one of the important causes of mortality in all populations. Some herbal drugs have been utilized for the treatment of MI. This research was designed to study the cardioprotective effects ofSemelil (ANGIPARS ™), a novel herbal drug, in male rabbits. Twenty New Zealand white rabbits were randomly allocated into four groups: control vehicle, sham vehicle, ischemia vehicle, andSemelil 10  mg/kg. Ischemia was created by complete occlusion of the left anterior descending coronary artery (LADC).Semelil was intraperitoneally injected for 2  weeks in the treatment group. Functi...
Source: Comparative Clinical Pathology - September 22, 2016 Category: Pathology Source Type: research

Genotypic analysis of Giardia duodenalis in children at Egypt
AbstractGiardia duodenalis is among the most common and frequent intestinal protozoan infecting Egyptian children. The present study aims to identify the genotypic features ofG. duodenalis among children fecal samples complaining of diarrhea at Beni-Suef Governorate, Egypt, and to study the association betweenGiardia assemblages and clinical presentation of the disease among this category. One hundred thirty diarrheic stool samples were subjected to direct stool examination, and positive samples forGiardia were subjected to copro-DNA extraction and amplification targeting the triose phosphate isomerase (tpi) gene using nes...
Source: Comparative Clinical Pathology - September 22, 2016 Category: Pathology Source Type: research

Correlation between γ-globulins and Encephalitozoon cuniculi immunoglobulins in suspected infected rabbits
In this study, 76 symptomatic rabbits were divided in three groups based on their Ig results: IgM+ IgG+, IgM-IgG+ and IgM-IgG-. By statistical analysis total serum protein was proved not to be effective in predicting an antibody response, in contrast the γ-globulin ranges from serum electrophoresis can predict whether the patient is positive for IgM and/or IgG. In fact, a significant quantitative correlation between percentage of γ-globulins and positive IgM and IgG serum forE. cuniculi was established in symptomatic rabbit. These values can be useful to perform a screening both for symptomatic and asymptomatic...
Source: Comparative Clinical Pathology - September 21, 2016 Category: Pathology Source Type: research

Neem ( Azadirachta indica ) leaf and kernel and their effects on production and physiology of poultry: a review
AbstractThe heavy demand for cereal grains and legumes by industries, humans and animals has given rise to increase in their prices and consequently in the costs of livestock feeds. There is a need therefore for the exploitation of other alternative noncompetitive feed resources in other to replace cereal grains and legumes in poultry diets. Many tropical plants produce leaves and seeds that are little known in poultry nutrition. The identification, processing and use of such leaves and seeds in poultry diets will go a long way in reducing the dependence on conventional feedstuffs. Neem is a tropical tree plant that yields...
Source: Comparative Clinical Pathology - September 16, 2016 Category: Pathology Source Type: research

Alkaloid extracts from shea butter and breadfruit as potential inhibitors of monoamine oxidase, cholinesterases, and lipid peroxidation in rats ’ brain homogenates: a comparative study
AbstractShea butter (Vitellaria paradoxa) and breadfruit (Treculia africana) are wild edible plants commonly consumed across the world. Unfortunately, these plants are currently going into extinction despite their potential biological properties. The actions of alkaloid extract from shea butter nut and breadfruit on some central nervous system biomarkers (monoamine oxidase (MAO), acetylcholinesterase (AChE), butyrylcholinesterase (BChE)) linked to some neurodegenerative diseases were investigated in this study. The radical scavenging and metal chelating abilities of the extract were also studied. Alkaloid extracts were obt...
Source: Comparative Clinical Pathology - September 8, 2016 Category: Pathology Source Type: research

Coexisting of titanium dioxide nanoparticles and diazinon on histopathology of common carp ( Cyprinus carpio )
In conclusion, this research suggested that the coexistence toxicity of diazinon and TiO2 NPs on the gill and intestine histopathology of common carp was higher than the diazinon and TiO2 NPs alone. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - September 6, 2016 Category: Pathology Source Type: research

The phylogenetic similarity of hydatid cyst isolated from humans and sheep in Ilam Province southwest of Iran
AbstractHydatid cyst is a chronic zoonotic disease caused by the larval stage of the dog tapeworm,Echinococcus granulosus. To identify genotype of hydatid cysts of human and sheep jackal in Ilam Province (South West of Iran), the PCR-RFLP and DNA sequencing were used. A total of 10 human and 20 sheep protoscoleces hydatid cyst samples were collected from different hospitals and slaughterhouses. Then, the gene of cox1 of mitDNA of the parasite was amplified and PCR products were cut usingAluI and HpaII restriction enzymes. Finally, a number of PCR products were bi-directionally sequenced. Based on the DNA sequencing and PCR...
Source: Comparative Clinical Pathology - September 5, 2016 Category: Pathology Source Type: research

Physicochemical and structure-activity properties of piroxicam —a mini review
AbstractThe neutral bidentate ligand coordinated to the metal ion through pyridyl-N and carbonyl of the amide moiety causes protonation/deprotonation equilibria (tautomerism) of piroxicam. The penetration of piroxicam through blood –brain barrier (BBB) may be by redox chemical delivery system linking it to the lipophilic dihydropyridine carrier creating a complex with carboxylic acid that transverses the BBB. The complex is enzymatically oxidized to the conic pyridinium salt. Subsequent cleavage of the drug from the pyridini um carrier leads to sustained drug delivery in the brain parenchyma. Brain uptake of piroxica...
Source: Comparative Clinical Pathology - August 12, 2016 Category: Pathology Source Type: research

Dietary docosahexaenoic acid to eicosapentaenoic acid ratios effects on hemato-immunological and plasma biochemical parameters in silvery-black porgy ( Sparidentex hasta ) juveniles
AbstractAn 8-week feeding experiment was conducted in juvenile silvery-black porgy (Sparidentex hasta) (initial mean weight 20.1  ± 0.1 g, mean ± SD) to estimate the optimum dietary docosahexaenoic to eicosapentaenoic acid (DHA/EPA) ratio. Triplicate groups of fish (12 fish in each replicate) were kept into 15 cylindrical polyethylene tanks (250 L) at 18.7 °C and handfed by one of the five diets which formulated wit h graded ratios of DHA/EPA (0.2, 0.4, 0.9, 2.0, 3.3) two times daily. In the fillet and liver, DHA and DHA/EPA ratios significantly increased with increasing...
Source: Comparative Clinical Pathology - August 9, 2016 Category: Pathology Source Type: research

Diffuse type testicular seminoma in a stallion
AbstractA 7-year-old stallion with progressive left testicular enlargement was presented. Grossly, the excised testicle measured 25  × 15 × 12 cm and weighed 3.7 kg. It was multinodular with a gray-white surface; however, the right testis was normal. Histologically, the neoplastic cells were disseminated diffusely in the tumoral stroma with a minimal fibrovascular stroma. Neoplastic cells were round to polygonal with abu ndant eosinophilic cytoplasm and large round to oval vesicular or hyperchromatic nucleous with a single prominent nucleolus. Immunohistochemically, the neoplastic ...
Source: Comparative Clinical Pathology - August 8, 2016 Category: Pathology Source Type: research

Some hematological reference values estimated by the reference values advisor in the Iraqi Awassi sheep
AbstractThe hematological parameters in domestic animal can be affected by several factors such as the sex, age, breed, and nutrition. There is a few published data on hematological reference values of the Iraqi Awassi sheep. Hence, the study aimed to determine hematological reference interval (RI) for the Awassi sheep to establish a basis for clinical interpretation. In the present study, a total 130 healthy Awassi sheep for both sex with two ages (2 –5 years) were selected randomly from five regions (Shula, Abu-Ghraib, Mahmoudia, Yousifia, and Alameel) West and South of Baghdad during the period from December ...
Source: Comparative Clinical Pathology - August 8, 2016 Category: Pathology Source Type: research

Effect of supplementation of newborn piglets with spray dry blood plasma on weight gain and serum biochemical variables
AbstractThe aim of this study was to evaluate the effect of ​spray dry blood plasma (SDBP) supplementation for newborn piglets on performance, seric biochemical variables, total antioxidant levels, and nitric oxide. Thus, 20 newborn piglets, with lower body wheith (1.179 ± 0.276 kg) were distributed in an experimental design with five randomized blo cks (females were considered block), with two treatments and ten repetitions. The treatment consisted to provide 10 g of SDBP (group T), previously diluted at 1:4 (blood plasma/water) administered for the first hour post-birth of the piglet...
Source: Comparative Clinical Pathology - August 4, 2016 Category: Pathology Source Type: research

Xenograft of Mus musculus ovary in the uterus of Rattus norvegicus after induction of pseudopregnancy
AbstractXenograft of gonads such as ovarian tissue can preserve fertility in patients at risk of infertility after radiotherapy or chemotherapy and also is valuable in preserving fertility in mammalian species. The purpose of this investigation was to preserve mouse (Mus musculus) ovarian tissue after xenografting in uterine lumen of rat (Rattus norvegicus) immunoprivileged by pseudopregnancy. Ten adult female Balb/c mice and seven female and four male Sprague-Dawley rats were enrolled. Seven mice were selected for transplantation, and three mice were euthanized and their ovaries were removed as controls. Female Sprague-Da...
Source: Comparative Clinical Pathology - August 3, 2016 Category: Pathology Source Type: research

Investigating temporal changes and effects of elevation on the prevalence of a rickettsial blood parasite in red-backed salamanders ( Plethodon cinereus ) in Virginia, USA
Abstract Amphibians are host to a number of vector-transmitted blood parasites. One parasite for which there is little information is a recently described intracellular bacteria in the order Rickettsiales , found in red-backed salamanders ( Plethodon cinereus ) in the eastern USA. This parasite was observed in 16.7  % of individuals across three states in the summer of 2008. Here, we resampled one of the locations from the 2008 study (Mountain Lake, Virginia, where site-level prevalence was 22 %) to determine if the incidence of infection has changed in 5 years. We also examined red-backed salamanders at se...
Source: Comparative Clinical Pathology - July 28, 2016 Category: Pathology Source Type: research

Assessment of gastrointestinal helminths among house mice ( Mus musculus) caught in the north-west of Iran, with a special view on zoonotic aspects
The objective of this study was to assess parasitic infection and associated risk factors among house mice caught in West Azarbaijan, north-west of Iran, with a special view on zoonotic aspects. One hundred and fifty six captured rodents were individually anesthetized with ether and dissected. The whole gastrointestinal tract was excised and examined for the presence of parasites. Of 156 examined mice, 43 (27.6  %) were found positive for gastrointestinal parasite. The overall, three Nematoda species; Syphacia obvelata (11.5  %), Syphacia muris (1.9  %), and Aspicularis tetraptera (1.3  %) and two Ce...
Source: Comparative Clinical Pathology - July 27, 2016 Category: Pathology Source Type: research

Biebersteinia multifida DC a potential savior against testicular torsion-induced reperfusion injury
Abstract The study aimed to investigate the potential therapeutic effect of the methanolic extract of Biebersteinia multifida DC on ischemia –reperfusion injury induced by testicular torsion/detorsion in rats. Forty adult male Sprague-Dawley rats were randomly divided into four groups. Sham operation group, torsion/detorsion group plus saline, and torsion/detorsion groups plus 75 and 150 mg/kg doses of DC extract (administered intrape ritoneally 15 min before detorsion). Testicular ischemia was induced via keeping the left testis under 720° counterclockwise torsion for 2 h; afterwards, detorsion ...
Source: Comparative Clinical Pathology - July 26, 2016 Category: Pathology Source Type: research

Phylotyping and ColV plasmid-associated virulence genotyping of E. coli isolated from broiler chickens with colibacillosis in Iran
Abstract Avian pathogenic Escherichia coli (APEC) strains are responsible for significant losses to the poultry industry in many parts of the world. Although, many serotypes of E. coli are non pathogen, a relatively few number of serotypes carry virulence genes which make them damaging for chicken and other poultry. Previously, high prevalence of virulence genes carried on plasmid and/or chromosomal DNA has demonstrated in APECs obtained from different countries. The aim of the present study is characterization of E. coli isolated from broiler chickens with pericarditis and femoral head necrosis (FHN) associated with c...
Source: Comparative Clinical Pathology - July 26, 2016 Category: Pathology Source Type: research

PCR and PCR-RFLP genotyping of Staphylococcus aureus coagulase gene: convenience compared to pulse-field gel electrophoresis
Abstract Staphylococcus aureus belongs to the group of major contagious mastitis pathogens. We aimed to evaluate the usefulness and the convenience of the coagulase ( coa ) gene polymorphism to type S. aureus from bovine mastitis milk samples. A total of 22 S. aureus strains were collected from bovine cases of mastitis in four veterinary practices in the Rhone-Alpes region of France and were assessed using coagulase gene ’s polymerase chain reaction (PCR) followed by Alu I PCR-restriction fragment length polymorphism (PCR-RFLP). Coa gene PCR produced six profiles. The Alu I PCR-RFLP displayed nine profiles, givi...
Source: Comparative Clinical Pathology - July 25, 2016 Category: Pathology Source Type: research

A prospective comparison of brush cytology before and after biopsy in diagnosis of gastric malignancies
Abstract Gastric malignancies are the third most common causes of cancer-related death in the world. Here, we aimed to compare brush cytology before and after biopsy in the diagnosis of gastric malignancies. This prospective study included 74 cases referred to endoscopy clinic for suspected gastric lesions during 1  year. In 38 cases, brush cytology was done before and in 36 cases it was done after biopsy. All the cytology and biopsy slides were randomly coded and reviewed by a pathologist blindly without any knowledge of the surgical pathology result or clinical data of the patients. Diagnostic accuracy of b rush cy...
Source: Comparative Clinical Pathology - July 25, 2016 Category: Pathology Source Type: research

Evaluation of the hepato-protective potential of Sarcocephalus latifolius leaf methanol extracts in paracetamol-induced liver damage of mice
Abstract Sarcocephalus latifolius , the African peach, is one greatly cherished, eaten and widely distributed shrubs mainly in the closed woodland savannah, and has been used to treat diverse diseases in some African and Asia populations. In Southern Nigeria, ‘herbal doctors’ include its leaves in decoctions given to patients of varying degrees of suspected liver damage. However, there is no scientific substantiation of their claim and use in literature. To evaluate the possible hepato-protective activity of S. latifolius leaves extract in paracetamol-induced hepato-compromised animal model as information is ...
Source: Comparative Clinical Pathology - July 25, 2016 Category: Pathology Source Type: research

Breed and seasonal variations in erythrocyte osmotic fragility of goat kids raised in semi-arid savannah
In conclusion, breed and season dramatically influence the susceptibility of erythrocytes of kids to hypotonic haemolysis with heat stress during the HDS causing decrease in membrane integrity in both breeds of goats. However, sexual immaturity may b e responsible for lack of sexual dimorphism in EOF in both breeds. The use of mitigation techniques and prevention of additional stress factors such as weaning is recommended during the peak of the HDS. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 24, 2016 Category: Pathology Source Type: research

Immune-mediated hemolytic anemia in a hypereosinophilic dog with intestinal T-cell lymphoma
Abstract A 13-year-old, 21-kg, female, mixed breed dog was presented with multifocal skin rashes, abdominal distension, and vomiting for several days. Radiographic images displayed the small intestine thickening. On exploratory laparotomy, a wall thickness of the jejunum and ileum about 15  cm long, with an adhesive 1-cm-diameter nodular mass was found. Histopathologically, the mass was diagnosed as lymphoma. The lymphoid cells were positive for CD 3, but were negative for CD 79α on immunohistochemical staining. Hematological profiles revealed severe hypochromia, autoagglutination, and eosinophilic left shift. ...
Source: Comparative Clinical Pathology - July 22, 2016 Category: Pathology Source Type: research

Acute renal failure by use of dipyrone in Cavia porcellus
Abstract Renal failure is a condition that occurs when one or more functions of the kidneys are changed and in guinea pig (Cavia porcellus) its occurrence is uncommon but always lethal. This paper reports the occurrence of acute renal failure in a patient mistakenly treated with dipyrone for 5 days in a veterinary hospital of UNOESC/Xanxerê-SC, presenting apathy, anorexia and hematuria diagnosed through laboratory tests. The use of dipyrone generated an eminent intoxication condition and the specific knowledge of therapy and familiarity with exotic animal species is extremely important to avoid cases of...
Source: Comparative Clinical Pathology - July 17, 2016 Category: Pathology Source Type: research

Role of fibronectin in the process of human intestinal cell disruption during E. histolytica infection
We examined the mechanism using topology networks and functional analysis and the result showed links to 765 proteins, of which fibronectin was predominant in the network. Fibronectin interacts with ubiquitin conjugate, insulin-like factor binding protein-3, and integrin alpha chain-2 which play pivotal roles in maintaining cell cycle, survival, and apoptosis. Therefore, inhibition of the protein results in intestinal cells losing the ability to maintain homeostasis mechanism by which the disruption of intestinal cell occurs. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 17, 2016 Category: Pathology Source Type: research

Electron microscopic evaluation of milk thistle, chicory, and vitamin E effects on gentamicin-induced nephrotoxicity in rat
In conclusion, milk thistle, chicory, and vitamin E, individually, can reduce kidney damage caused by gentamicin, but they have a greater effect in reducing damage if used together. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 13, 2016 Category: Pathology Source Type: research

C hanges in serum biochemical parameters and oxidative stress biomarkers in grazing cattle
In this study, blood was collected from healthy cows kept in a pen (non-grazing) for 1 week after which they were subjected to grazing for another week, to determine the effect of grazing on serum biochemical parameters and oxidative stress biomarkers. Grazing significantly increased (p 
Source: Comparative Clinical Pathology - July 11, 2016 Category: Pathology Source Type: research

The growth kinetic, differentiation properties, karyotyping, and characterization of adipose tissue-derived stem cells in hamster
Abstract Multilineage properties of mesenchymal stem cells (MSCs) cause their contribution in repair of different tissue. Adipose tissue is considered a good source of MSCs because of its abundance and easy access. The aims of this study were to characterize and determine the differentiation properties and growth kinetics of adipose tissue-derived stem cells (AT-SCs) from Syrian hamster. Fat tissue was isolated from the abdominal and pelvic regions of the hamster and by using type I collagenase, and cells were isolated and cultured. AT-SCs were characterized by morphology, cell surface markers, and adipogenic and ...
Source: Comparative Clinical Pathology - July 11, 2016 Category: Pathology Source Type: research

The effect of hydroalcoholic extract of Mangifera indica on induced osteoarthritis of knee in male guinea pigs
Abstract Osteoarthritis (OA) is one of the top six diseases for which complementary and alternative medicine is used. Among the herbal products, mango could be regarded as a potential natural remedy for OA due to its anti-inflammatory characteristics. The aim of the present study was to evaluate the healing effect of mango on OA of the knee. Eighteen male guinea pigs were chosen, and anterior cruciate ligament transection was performed for them to induce OA for the animal models. Then, they were recruited in four treatment groups (n = 5): (a) oral treatment, (b) intra-articular injection treatment, (c) p...
Source: Comparative Clinical Pathology - July 10, 2016 Category: Pathology Source Type: research

D -dimer concentrations in dogs with kidney disease with or without protein-losing nephropathy
In conclusion, the detection of increased D-dimers in the context of impaired kidney function should be cautiously interpreted in the dog and should not be invariably assumed to imply the presence of a state of hypercoagulability. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - July 10, 2016 Category: Pathology Source Type: research

Point-of-care blood gases and critical care blood chemistry and haematology reference values in captive gyr falcons ( Falco rusticolus )
Abstract Blood samples were obtained from a total of 94 clinically normal captive gyr falcons (Falco rusticolus) divided into two groups. The first group comprised 44 gyr falcons (35 females and 9 males). These falcons were 5 to 6 months old and were destined for the sport of falconry. The second group comprised 50 gyr falcons, (26 females and 24 males). These falcons were 2 to 9 years old and were part of the captive breeding programme at the Falcon Department, Wildlife Division, Wrsan, Abu Dhabi, United Arab Emirates (UAE). The samples were obtained in order to determine blood gases and critical care b...
Source: Comparative Clinical Pathology - July 5, 2016 Category: Pathology Source Type: research

Neoplasia in flat head grey mullet, Mugil cephalus Linnaeus, 1758 (Perciforms: Mugilidae): two case reports of myxoinflammatory fibroblastic sarcoma and leiomyoma
Abstract The presence of cutaneous neoplasms is reported in two cases of wild female grey mullet (Mugil cephalus) from Pazhayar and Parangipettai fish landing centre, southeast coast of India. A gross morphological examination case 1 revealed obvious reddish white, solitary and fleshy appearance of tumour located on behind the right-side of the anal fin, and the overlying epidermis was ulcerated, hyperemic and hyperchromatic. The tumour had a gelatinous consistency and released jelly-like matters. The tumour-affected case 2 revealed well-circumscribed, solitary and reddish fleshy appearance of tumour located on th...
Source: Comparative Clinical Pathology - July 5, 2016 Category: Pathology Source Type: research

Sorghum stem extract modulates Na + /K + -ATPase, ecto-5 ′-nucleotidase, and acetylcholinesterase activities
This study sought to assess the antioxidant activity and effects of phenolic extract from sorghum stem ( Sorghum bicolor ) on some cholinergic (acetylcholinesterase (AChE)) and purinergic (Na + /K + -ATPase and ecto-5 ′-nucleotidase) enzymes associated with neurological conditions. Phenolic-rich extract was prepared using methanol: 1 N HCl (1:1, v / v ) mixture and characterized using high-performance liquid chromatography-diode array detector (HPLC-DAD). In vitro tests were used to investigate the effects of the phenolic extract on AChE, Na + /K + -ATPase, and ecto-5 ′-nucleotidase activities. Furthermore...
Source: Comparative Clinical Pathology - June 30, 2016 Category: Pathology Source Type: research