The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia
Abstract Paravertebral block is a method of regional block used to make pain relief and decrease systemic analgesic requirements for patients undergoing breast cancer surgery. This work aimed to evaluate the effect of paravertebral block on monocyte phenotype and their functions after surgery for cancer breast patients. One hundred patients which the American Society of Anesthesia (ASA) I–III planned for breast cancer surgery were allocated into two groups. Group A (n = 50) received general anesthesia in a standard technique. Group B (n = 50) received paravertebral block T2–...
Source: Comparative Clinical Pathology - May 8, 2015 Category: Pathology Source Type: research

Immunohistochemical expression of manganese-superoxide dismutase (Mn-SOD) and triose-phosphate isomerase (TPI) in canine mammary gland tumor
Abstract Canine tumors, especially canine breast cancer, share common properties, including metastatic pattern, histopathological characteristics, and biological features with human cancers. Therefore, canine mammary gland tumor is deemed as an appropriate model for human breast cancer. We investigated expression of two important tumor antigens, including manganese superoxide dismutase (Mn-SOD) and triose-phosphate isomerase (TPI) in canine mammary gland tumors using immunohistochemistry analysis. Expression of these proteins were evaluated in tumor (29 malignant and 16 benign samples) and normal (n =&thins...
Source: Comparative Clinical Pathology - May 7, 2015 Category: Pathology Source Type: research

Effect of exercise on erythron, heart and respiratory rates in horses vaccinated against equine herpes virus 4/1 and equine influenza virus
Abstract Limited data are available for the influence of physical training in jumping horses at the background of booster vaccination. The aim of the study was to establish the effects of physical exercise applied at the background of booster vaccination against equine herpes virus 4/1 (EHV 4/1) and equine influenza virus (EIV) on changes in the erythron, breathing and pulse rates of horses. The differences were investigated between vaccinated and exercised horses and vaccinated-only controls. Fifteen Hanoverian geldings were distributed into 3 groups: group А, 3 non-vaccinated animals; group В, 6 vaccinated and...
Source: Comparative Clinical Pathology - May 6, 2015 Category: Pathology Source Type: research

Sub-acute toxicity of lantadenes isolated from Lantana camara leaves in guinea pig animal model
Abstract The sub-acute toxicity of lantadenes isolated from Lantana camara leaves was studied in guinea pig laboratory animal model. A total of 20 guinea pigs of either sex were divided into five groups. Group I served as control, while groups II, III, IV, and V were orally administered lantadenes at the dose rate of 100, 50, 25, and 12.5 mg/kg body weight (bw), respectively for 28 days. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, total bilirubin, blood urea nitrogen, and creatinine were estimated in serum. The effect of lantadenes on lipid ...
Source: Comparative Clinical Pathology - May 3, 2015 Category: Pathology Source Type: research

A serological survey on Borrelia burgdorferi infection among companion dogs in Ahvaz district, southwestern Iran
Abstract Canine Lyme borreliosis is one of the most common tick-transmitted diseases in the world. The infection is caused by spirochetal bacteria from the genus Borrelia burgdorferi. The principal vectors are various species of slow-feeding hard ticks of the Ixodes complex. Dogs are the most infected animals among the pets. The status of infection due to Borrelia in dogs remains unknown in many areas of Iran. Few studies have been reported on the distribution of this disease in the Iran dog’s population, so the aim of this survey was to evaluate the seroprevalence of Borrelia infection in companion dogs in ...
Source: Comparative Clinical Pathology - May 3, 2015 Category: Pathology Source Type: research

Antioxidant enzymes gene polymorphisms and hepatocellular carcinoma in hepatitis C virus-infected Egyptian patients
Abstract Oxidative stress is an imbalance between production and elimination of reactive metabolites of oxygen and nitrogen, in favor of their production leading to potential damage. During oxidative stress, biologically important molecules and cells can be damaged, and this can be significant in the pathogenesis of many diseases. Reactive oxygen species (ROS) and ROS-induced cytokines are known to trigger the apoptosis of some hepatocytes and therefore contribute to inflammation, regeneration, fibrogenesis, and carcinogenesis. The enzymes generally considered to be the frontline defense against ROS are catalase ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Baseline blood values for healthy free-ranging green sea turtles ( Chelonia mydas ) in Puerto Rico
We report packed cell volume, plasma biochemistry, and protein electrophoresis reference interval values for juvenile and subadult green sea turtles. Development of site-specific baseline blood health parameters for wild, healthy sea turtle populations is an important factor in creating effective management protocols and enhances our ability to understand the effects of anthropogenic and environmental changes on sea turtle health. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Prostatic carcinoma in a dog: a new approach by using the Gleason system
We describe the pathological features of prostatic carcinoma in a dog using the Gleason system where the related score was estimated as 7 (3 + 4). Perineural invasion was also noted, and the neoplastic cells showed no reaction with PSA antibody but were positive for Ki-67 and CK-7 and IHC staining. The expression of Ki-67-SI was 3.28 %. Traditional canine prostate cancer classification could not cover the obscured characteristics of our case; therefore, a more descriptive histological pattern system using the Gleason grading is recommended. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Serological study of toxoplasmosis in women with previous history of abortion at Hamedan’s medical centers during 2012–2013
Abstract Toxoplasma gondii is one of the major agents of infectious abortions and, due to its worldwide distribution, can threat healthy pregnant women who had no previous exposure to this parasite. The present study was designed to determine the titer of anti-toxoplasma antibody in women with history of abortion visiting Hamedan’s medical centers during 2012–2013. Blood samples were collected from 300 mothers with previous history of abortion coming to the provincial medical centers during the period spanning October 2012 to June 2013. The sera were isolated and subjected to evaluate the ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis
Abstract The aim of this study was to assess the pathophysiological importance of lipid profile, acute phase proteins, proinflammatory cytokines and oxidative stress markers in sheep with pneumonic pasteurellosis. Blood samples were collected from 36 Pasteurella multocida-infected sheep, together with 20 healthy controls. Samples for bacteriological examination (nasal swabs, bronchoalveolar lavage) were collected from all animals and subjected to bacteriological examinations. Moreover, heart blood and lung samples were collected from the dead pneumonic sheep and subjected also to bacteriological examinations. A l...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Comparative study of therapeutic protocols used in the treatment of infections caused by rickettsiales in dogs
Abstract Tick-borne diseases are caused by rickettsiales microorganisms of the genera Anaplasma and Ehrlichia, and often are diagnosed in dogs (Canis familiaris). Therapeutic protocols include antibiotics of different classes, mainly tetracyclines and derivatives. This comparative study contrasts two treatment protocols routinely used in veterinary medicine to treat Rickettsiosis and assesses the respective hematological changes. Dogs used for this research were divided in two groups: group 1, treated with long-acting oxytetracycline 15 mg kg−1 and group 2, treated with doxycycline 10 mg ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

A spontaneous case of spinal cord degeneration in an adult dairy goat
Abstract A 2-year–old Saanen female goat, which showed a 10-day history of neurological deficit symptoms encompassing posterior weakness, difficulty in rising, and eventually inability to rise, was euthanatized because of poor prognosis. Histopathology revealed the presence of spinal cord degeneration characterized by Wallerian-type degeneration of myelinated nerve fibers in the white matter in association with myelin sheath degeneration, reactive astrocytosis, fibrillary astrogliosis, and capillary proliferation while sparing neurons in the gray matter horns. Lesions were bilateral and more marked in the l...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Collagen metabolism and antioxidant status in the paw tissue of type II collagen-induced arthritic rats—protective role of alginic acid isolated from Sargassum wightii
Abstract The aim of the present study was to evaluate the effects of alginic acid isolated from the brown algae, Sargassum wightii, on collagen metabolism and antioxidant status in the paw tissue of rats in experimental arthritis. To induce arthritis, rats were immunized with type II collagen (2 mg/ml) intradermally. Alginic acid (100 mg/kg) and indomethacin (3 mg/kg) were administered orally for 21 days. The severity of arthritis was assessed by measuring paw edema volume and histology of hind paw tissue. Markers of oxidative stress, protein-bound carbohydrates in plasma, and urine and degrad...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Resistance to different generations of quinolones in Streptococcus pneumoniae strains isolated from hospitals in Shiraz
Abstract Streptococcus pneumoniae is a leading cause of respiratory tract infections, including pneumonia. In recent years, due to the incidence of resistance to commonly used antibiotics, quinolones have been taken into consideration in treatment of S. pneumoniae. The present study aimed to investigate the quinolone resistance rate among pneumococcus isolates from Shiraz. This cross-sectional-descriptive study was performed on 45 strains of S. pneumoniae isolated from clinical samples. Initially, using biochemical tests and PCR technique with specific primers of lytA gene, S. pneumoniae strains were ...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Isolation of Theileria lestoquardi infected cells and using for immunization of sheep without attenuation
In this study sheep inoculated with low passage (without attenuation) of this isolate showed no clinical signs of theileriosis, which shows the significance of this cell line to develop a tissue-culture vaccine against malignant ovine theileriosis. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Effects of pentoxifylline on skeletal muscle injury induced by acute hindlimb ischaemia–reperfusion: a histopathological study in a rat model
This study evaluated the effects of pentoxifylline, a methylxanthine derivative, on skeletal muscle injury after acute hindlimb ischaemia–reperfusion. Twenty male Wistar rats were allocated randomly into two groups: ischaemia–reperfusion and ischaemia–reperfusion + pentoxifylline. All animals underwent 2 h of ischaemia by occlusion of the femoral artery followed by 24 h of reperfusion. Rats in ischaemia–reperfusion + pentoxifylline group were given a dose of intraperitoneal pentoxifylline injection 40 mg/kg immediately before reperfusion. After the reperfus...
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Outbreaks of thrush in pigeons in Punjab State of India
Abstract Due to high monetary turnover in business, white pigeon keeping for game purposes is gaining more popularity in Punjab. Overcrowding and poor management by undertrained naive farmers make these birds more susceptible to diseases not known so far in this region. A farmer reported that about a hundred pigeons were unable to feed properly and regurgitate feed. Birds lost body condition gradually, and three among these died. Both alive and dead pigeons were presented to the Veterinary Clinical Complex (VCC) for detailed examination. All these pigeons were found to be cachectic with wasting of breast muscles....
Source: Comparative Clinical Pathology - May 1, 2015 Category: Pathology Source Type: research

Clinical aspects, morphometry, and morphology of blood cells of the emu ( Dromaius novaehollandiae ) reared in captivity
This study evaluates the clinical aspects, the morphometry, and the morphology of emu blood cells reared in captivity, in the city of Campos dos Goytacazes, state of Rio de Janeiro, Brazil. Fifteen animals of either sex reared in the scientific nursery of Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) were clinically examined before egg hatching. This group included three males, which also started the egg incubation period, that were analyzed during two periods (during incubation and off incubation). Blood samples were collected, and smears were prepared and stained for morphometric and morphological analys...
Source: Comparative Clinical Pathology - April 30, 2015 Category: Pathology Source Type: research

Evaluation of Bacillus licheniformis and Bacillus subtilis as probiotic and Fe ion effects on haematological parameters of rainbow trout, Oncorhynchus mykiss frys
In this study, the effects of a commercial probiotic containing Bacillus licheniformis and Bacillus subtilis along with feriron solution were investigated on the haematological parameters of rainbow trout frys. The experiment was carried out in eight treatments and one control group for a period of 60 days after beginning of active feeding. In the experimental treatments, the frys were fed with dry diets containing various ratios of probiotic and feriron solution. The haematological parameters including number of white blood cells (WBCs) and red blood cells (RBCs), haemoglobin concentration (Hb), haematocrit percent, ...
Source: Comparative Clinical Pathology - April 24, 2015 Category: Pathology Source Type: research

SDF-1(CXCL12) polymorphisms in Egyptian patients with systemic lupus erythematosus (SLE): a pilot study
Abstract SDF-1(CXCL12) is a chemokine that plays an important role in the regulation of migration, proliferation, and differentiation of hematopoietic cells, as well as being involved in the homeostatic and inflammatory traffic of leukocytes. It was suggested that CXCL12 is a key molecule in the development of autoimmunity in the murine model of lupus. It has been demonstrated that SDF-1 has a G801A transition at position 801 in the 3′-untranslated region of the transcript, known as SDF-1-3′G801A. This polymorphism may have an important regulatory function via an increase in the biosynthesis of SDF-1 p...
Source: Comparative Clinical Pathology - April 24, 2015 Category: Pathology Source Type: research

Reference intervals for haematological and plasma biochemical parameters in sobaity sea bream juveniles ( Sparidentex hasta , Valenciennes 1830)
Abstract Sobaity sea bream (Sparidentex hasta) is distributed in the Western Indian Ocean, Persian Gulf and Oman Sea. For monitoring health status in this species in an aquaculture condition, standard reference values for haematological and plasma biochemical parameters are indispensable. Heparinized blood samples were collected from 143 healthy sobaity sea bream juveniles raised in cylindrical polyethylene tanks with filtered running seawater. Haematologic reference intervals were as follows: red blood cell (RBC) count 1.67–2.2 × 106 μL−1, total white blood cell (WBC) count...
Source: Comparative Clinical Pathology - April 24, 2015 Category: Pathology Source Type: research

Effect of Kalpaamruthaa on immunosuppression of both humoral and cell-mediated immunity in DMBA-induced mammary tumours of rats
Abstract The present study was carried out to elucidate the protective effect of Kalpaamruthaa on improving 7,12-dimethylbenz(a)anthracene (DMBA)-induced immunosuppression of both humoral and cell-mediated immunity in mammary carcinoma-induced rats. Breast cancer was induced in rats by administering DMBA orally (25 mg/rat) as a single dose. After 90 days of induction, SA (200 mg/kg body weight) and KA (300 mg/kg body weight) were administered for 14 days, by gastric intubation. Several immunotoxicological assays such as T cell rosette delayed type hypersensitivity (DTH) response, migration...
Source: Comparative Clinical Pathology - April 24, 2015 Category: Pathology Source Type: research

Hematological and bone marrow parameters in busulfan-treated rats: effects of silymarin extract
In this study, busulfan and bone marrow suppression were used to assess whether the effect of silymarin can suppress cell death, or at least reduce it. Forty-two Wistar male rats were fed with a standard diet, divided into six groups of eight rats and treated as follows. Group 1—control group (received nothing). Group 2—control silymarin, 175 mg/kg/day silymarin was gavage for 14 days. Group 3—anemic control group, 20 mg/kg/day busulfan was injected intraperitoneally (i.p.) for 14 days. Group 4—experimental group 1 received 20 mg/kg/day busulfan (i.p.) for 14 days, and ...
Source: Comparative Clinical Pathology - April 24, 2015 Category: Pathology Source Type: research

Detection of an active complement system in red-tailed phascogales ( Phascogale calura )
Abstract Very few assays that are used to assess the status of mammalian immunity have proved useful for assessment of marsupial health and/or diagnosis of disease. This is largely due to the lack of species cross-reactive reagents that underpin such experiments. To begin to address this deficit, we describe the activation of classical and alternative complement pathways of red-tailed phascogales (RTP; Phascogale calura). Using standard haemolytic assays, the existence of both complement pathways were established in RTP serum based on its ability to lyse unsensitised rabbit erythrocytes (RbE) and sensitised sheep ...
Source: Comparative Clinical Pathology - April 23, 2015 Category: Pathology Source Type: research

Hepatoprotective effect of ethanolic extract of whole plant of Andrographis paniculata against CCl 4 -induced hepatotoxicity in rats
Abstract Liver diseases pose a major challenge to international public health. Hepatic damage is associated with distortion of metabolic functions of the liver. The present study was carried out to evaluate the hepatoprotective effect of the ethanolic extract of whole plant of Andrographis paniculata in CCl4-induced hepatotoxicity in albino rats. Hepatotoxicity was induced in rats by single intraperitoneal injection of 30 % CCl4 suspended in olive oil (1 ml /kg b.w.) after every 72 h for 10 days (group II). Hepatotoxicity-induced rats were treated with 200 mg/kg b.w. of ethanolic extract o...
Source: Comparative Clinical Pathology - April 23, 2015 Category: Pathology Source Type: research

Prevalence of Brucella spp. in equines of Iran
In this study, blood samples were obtained by venous puncture and transferred to the laboratory under chilled conditions as quickly as possible. Sera from three horses were positive by Rose Bengal test (RBT) and tube agglutination tests (TAT), resulting in a prevalence rate of 2.5 %. The samples from two stallions showing positive reaction by RBT also showed positive results by TAT (positive reaction (PR) 2.6 %). The stallions testing positive displayed no clinical signs related to brucellosis. One mare had positive reactions in both tests, with no clinical signs (PR 2.3 %). Close contact between animals tha...
Source: Comparative Clinical Pathology - April 23, 2015 Category: Pathology Source Type: research

Apoptotic and cytotoxic effect of Semecarpus anacardium Linn nut milk extract on MCF-7 breast cancer cells
Abstract Semecarpus anacardium Linn nut milk extract (SA) has been used in Siddha system of medicine against various ailments. The present study was designed to evaluate the apoptotic and cytoprotective effect of the drug SA on MCF-7 breast cancer cell line. The effect of the drug SA on growth rates of MCF-7 cells were determined by trypan blue exclusion method whereas cytotoxicity was determined by lactate dehydrogenase (LDH) assay. In order to determine whether the drug-mediated cell death of cancer cells was either through apoptosis or necrosis, PI staining was done. We observed that the drug SA exhi...
Source: Comparative Clinical Pathology - April 23, 2015 Category: Pathology Source Type: research

The influence of carob powder on lipid profile and histopathology of some organs in rats
Abstract The present study aimed to investigate the effect of two levels of carob powder 10 and 20 % on lipid profile. Forty male Sprague–Dawley rats were divided into two groups. The first group was fed on a basal diet and acts as a control group. The second group was fed on a hyperlipidemic diet (a basal diet plus 10 % animal fat and 1 % cholesterol). After 6 weeks, the second group was divided into three even subgroups: subgroup 1 was fed on a hyperlipidemic diet, subgroup 2 was fed on a hyperlipidemic diet plus 10 % carob powder, and subgroup 3 was fed on a hyperlipidemic diet pl...
Source: Comparative Clinical Pathology - April 22, 2015 Category: Pathology Source Type: research

Damage and regeneration of small intestinal enterocytes under the influence of carrageenan induces chronic enteritis
This study examines morphological features of small intestine and evaluates apoptotic biochemical indices in homogenate of small intestine of rats after prolonged oral administration of carrageenan. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 15, 2015 Category: Pathology Source Type: research

Gastrointestinal parasites of vervet monkeys around Lake Hawassa recreational sites, southern Ethiopia
The objective of the present study was to assess the occurrence of gastrointestinal parasites in free-ranging vervet monkeys. A total of 140 free-ranging vervet monkeys found in the recreational areas of Hawassa city, Ethiopia, were examined for gastrointestinal parasitic infection from November 2013 to April 2014. For the identification of eggs and larvae of nematodes and cysts, oocysts and trophozoites of protozoa, faecal samples were subjected to direct smear and floatation techniques. Overall, out of 140 faecal samples examined, 75 % were found to harbour one or more gastrointestinal nematode parasites. The most f...
Source: Comparative Clinical Pathology - April 14, 2015 Category: Pathology Source Type: research

Rapid serodiagnosis of Trypanosoma evansi in dogs by colloidal dye immunobinding assay
Abstract Trypanosoma evansi causes significant mortality in dogs. Diagnosis of T. evansi infection can be done by different diagnostic methods including wet blood film examination, stained blood smear examination, advanced serological and molecular tests. In the present study, colloidal dye immunobinding technique was developed for diagnosis of T. evansi in dogs. In this technique, the whole cell lysate antigen was coated on the nitrocellulose membrane of the flow-through device. Protein A colloidal gold was used as detector. The evaluation of the technique was performed by comparing the known positive ...
Source: Comparative Clinical Pathology - April 14, 2015 Category: Pathology Source Type: research

Serum protein profile of hookworm infection in dogs
Abstract Hookworms are parasitic nematodes that cause anemia and intestinal infections in dogs, especially with large worm burdens. However, the serum protein profile of this parasitological disease is still poorly understood. The present study was design to evaluate 80 asymptomatic dogs (age; 8 months–2 years) to detect the presence of the hookworm thin-shelled, morulated eggs in faeces using faecal flotation and to evaluate the serum protein fractions determined by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Forty dogs had an elevated quantity of eggs in faeces ...
Source: Comparative Clinical Pathology - April 10, 2015 Category: Pathology Source Type: research

Topical co-administration of flaxseed and pistachio ointment promoted wound healing; evidence for histopathological features
This study was conducted to compare the traditional prescriptions of flaxseed and Pistacia atlantica oil in wound healing in rabbit. Eighty white New Zealand rabbits randomly allocated to five experimental groups (n = 16). Two full-thickness skin excisional wounds were created on the thoracolumbar region on each side of the dorsal midline in each rabbit. Group 1 was considered as control. Group 2 rabbits received nitrofurazone ointment (0.2 %, w/w). Group 3 animals were treated with P. atlantica hull (2 %) ointment. In group 4, rabbits received flaxseed ointment (2 %), and group 5 was treated w...
Source: Comparative Clinical Pathology - April 8, 2015 Category: Pathology Source Type: research

Mammary fibromatosis (desmoid tumor) in a dog: cytological, pathological, and immunohistochemistry studies
Abstract In the present study, we describe an ill-defined firm mass in the mammary tissue in an 8-year-old terrier dog. Fine-needle aspiration (FNA) cytology showed a moderate cellularity with numerous bland bare nuclei and proliferating fibroblasts in a bloody background. Occasional clusters of spindle cells also were present. Histopathologically, a proliferation of spindle cells without atypia forming or interlacing fascicles was observed. A variable amount of collagen was seen in the background. There was no evidence of mitotic figures. Immunohistochemical analysis presented that the neoplastic cells expressed ...
Source: Comparative Clinical Pathology - April 7, 2015 Category: Pathology Source Type: research

Mutation screening of exon 9 of DR5 receptor in patients with breast cancer using PCR-SSCP
In conclusion, these results suggest that the commonly found mutations in exon 9 of the DR5 gene may be one of the sensitive positions for tumorigenesis of the cancer. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 7, 2015 Category: Pathology Source Type: research

Canine nasal neuroendocrine carcinoma: case report with a brief review of the literature
Abstract A mass of approximately 5 × 5 × 5 cm was located on the bridge of the nose, nasal septum, turbinates, and soft and hard palates of a 1-year-old male terrier dog. The skin over the mass was alopecic and ulcerated. Histopathologically, the tissue sections consisted of sheets of neoplastic cells separated by narrow bands of fibrovascular stroma. The neoplastic cells were round to polyhedral with moderate, eosinophilic cytoplasm, and large clear nuclei. Mitotic figures were frequently observed. Immunohistochemically, the neoplastic cells were diffusely positive for...
Source: Comparative Clinical Pathology - April 2, 2015 Category: Pathology Source Type: research

Intraneural cavernous haemangioma of ulnar nerve and cubital tunnel syndrome
Abstract Cubital tunnel syndrome due to cavernous haemangioma of ulnar nerve is a rare condition. In the present study, we report a case of intraneural haemangioma of the ulnar nerve at the elbow in an Iranian woman. A 40-year-old woman with a left elbow pain, ulnar nerve thickness, intrinsic muscle atrophy of the hand and local tenderness was admitted in our hospital. Ultrasound of elbow soft tissue showed a hypoechoic tubular mass (15 × 10 mm) that seen along the ulnar nerve. With the aid of a microscope, removal of tumour with minimal damage to the nerve fascicles was accomplished. Hist...
Source: Comparative Clinical Pathology - March 31, 2015 Category: Pathology Source Type: research

The proposed mechanisms of radio frequency waves (RFWs) on nervous system functions impairment
Abstract There is a general concern regarding the hazardous health effects of exposure to radiofrequency waves (RFWs) emitted from wireless communication devices. RFWs generated by base transceiver station (BTS) and/or mobile phone have been reported to generate deleterious effects on nervous system, possibly through the non-thermal effects and thermal effects. However, most studies have focused on the non-thermal effects of RFW. In this review, we will consider effects of radiofrequency waves on impairment of nervous system functions. Available data indicate that RFW of relatively low intensity (specific absorption rate (SAR)
Source: Comparative Clinical Pathology - March 31, 2015 Category: Pathology Source Type: research

Concentrations of l -ergothioneine in follicular fluids of farm animals
Abstract The aim of this study was to evaluate the concentration of l-ergothioneine in follicular fluid of some domestic farm animals, such as mare, sheep, goat, sow, and unweaned lamb. The group of unweaned lambs was the cluster with the lowest mean concentration (0.12 ± 0.06 μmol/L), while the mares showed the highest mean values (3.16 ± 1.27 μmol/L). The mean levels in the sheep and goats were similar (0.72 ± 0.21 vs. 0.73 ± 0.50 μmol/L), while the mean values in the sows were slightly higher (1.04&thinsp...
Source: Comparative Clinical Pathology - March 31, 2015 Category: Pathology Source Type: research

Effect of Cannabis sativa extract on gastric acid secretion, oxidative stress and gastric mucosal integrity in rats
Abstract Studies were conducted in pylorus-ligated rats to investigate the effect of Cannabis sativa extract on gastric acid secretion, experimental gastric ulcer and on oxidative stress and inflammatory markers in the gastric mucosa. C. sativa (5, 10 and 20 mg/kg, expressed as Δ9-tetrahydrocannabinol) was administered subcutaneously daily for 4 weeks prior to pylorus ligation and different treatments. Under basal conditions, pretreatment with cannabis extract at doses of 5 and 10 mg/kg increased gastric acid secretion and induced minimally visible gastric mucosal lesions in the 4 h pylor...
Source: Comparative Clinical Pathology - March 27, 2015 Category: Pathology Source Type: research

Artifactual changes in the haematological values of cultured Hetroclarias hybrid blood stored at room and refrigerator temperatures
This study investigated the artifactual changes in the haematological values of Hetroclarias hybrid blood stored at room (30 °C) and refrigerator (5 °C) temperatures. Twelve apparently healthy fish weighing between 700 and 900 g were used for the study. Samples were immediately upon collection divided into two parts and haematological determinations carried out at six hourly intervals from 0 to the 36th hour and then at the 48th hour. Results showed significant reduction (p  0.05) at the refrigerator temperature. There were significant increases (p  0.05) were observed at ...
Source: Comparative Clinical Pathology - March 27, 2015 Category: Pathology Source Type: research

Normal haematological and blood biochemistry values of cultured Clarias gariepinus in Southeast, Nigeria
This study investigated the normal haematological and blood biochemistry profile of apparently healthy sharp-tooth catfish (Clarias gariepinus) raised in concrete ponds in Southeast, Nigeria. A total of 28 fish of two age ranges and equal sexes were used, and determination of haematological and blood biochemistry profiles followed standard procedures. Results obtained for the parameters assessed for the young adults and adults are summarized as follows: mean ± standard error: packed cell volume—31.22 ± 0.96 and 35.89 ± 1.44 %, haemoglobin concentrati...
Source: Comparative Clinical Pathology - March 26, 2015 Category: Pathology Source Type: research

On the occurrence of parasite, Lernaea cyprinacea from common carp, Cyprinus carpio farmed in ponds and rice fields
Abstract Lernaea cyprinacea and occurrence of Lernaeasis in farmed and wild warm water fishes is a major problem of aquaculture industry in Iran. The present study was conducted to compare the rate of L. cyprinacea infection between two separate populations of common carp, i.e., fish cultured in ponds and sampled from rice fields. To this end, fish sampling was done during 3 months in summer. After fish sampling, fish were transferred to lab for parasite study. Parasite incidence percent and density were calculated for two population carps. According to obtained results, the parasite incidence perc...
Source: Comparative Clinical Pathology - March 21, 2015 Category: Pathology Source Type: research

Immunoexpression of metalloproteinase 14 and tissue inhibitor of metalloproteinase 2 in colorectal carcinomas and lymph node metastases
Abstract Changes in metalloproteinase (MMP) and tissue inhibitor of metalloproteinases (TIMP) have been associated with tumor progression in colorectal cancer. However, the role of MMP-14 and TIMP-2 has yet to be determined. We investigated the differential expression of MMP-14 and TIMP-2 in colorectal carcinomas of the left and the right colon, as well as in mononuclear cells in primary tumors and their lymph node metastases. We performed an immunohistochemistry analysis of tumor samples obtained from 50 cases of colorectal cancer. We found that MMP-14 staining was positive in 100 % of cases, in contrast to ...
Source: Comparative Clinical Pathology - March 12, 2015 Category: Pathology Source Type: research

A survey on the status of equine skin tumors and associated epidemiological factors in Iran
This study demonstrated that there is a variation on the frequencies and type of equine skin tumors. Fibroma and sarcoid represented 38.4 and 34.8 %, respectively, as frequent tumors in the study. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 12, 2015 Category: Pathology Source Type: research

Antioxidant, hypolipidemic, and anti-angiotensin-1-converting enzyme properties of lemon ( Citrus limon ) and lime ( Citrus aurantifolia ) juices
Abstract Lemon (Citrus limon) and lime (Citrus aurantifolia) juices are used in folk medicine for the management of hypertension and other cardiovascular diseases, but the mechanism of action by which they exert their therapeutic action is unclear. The aim of this study is to investigate the effect of lemon and lime juices on angiotensin-1-converting enzyme (ACE) activity in vitro and investigate the hypocholesterolemic properties of the juices in a high-cholesterol diet rat model. The phenolic content and antioxidant properties of the manually squeezed juices were also determined. Lemon juice had higher total phe...
Source: Comparative Clinical Pathology - March 11, 2015 Category: Pathology Source Type: research

Granulosa cell proliferation in the gilt ovary associated with ovarian status and porcine reproductive and respiratory syndrome virus detection
Abstract The aims of the present study were to quantify the number of follicles and determine granulosa cell proliferation in the ovaries of gilts in relation to ovarian status and porcine reproductive and respiratory syndrome (PRRS) virus infection. Ovarian tissues were obtained from 37 Landrace × Yorkshire crossbred gilts aged 270.7 ± 24.5 days and weighing 144.5 ± 15.0 kg (27 cycling and 10 prepubertal gilts). PRRS virus was detected using immunohistochemistry, and the ovaries were classified as negative (n = 17) or positive (n =&thi...
Source: Comparative Clinical Pathology - March 11, 2015 Category: Pathology Source Type: research

Topical Rosemary officinalis essential oil improves wound healing against disseminated Candida albicans infection in rat model
This study evaluated the effect of a topical application of Rosemary officinalis essential oil ointment on a wound infected with Candida albicans, in rats. Sixty-three mature Wistar rats were allocated into three equal groups (n = 21 each): control, 2 % rosemary cream-treated and 4 % rosemary cream-treated. The rats underwent general anaesthesia, and a wound area with dimensions of 1.5 × 1.5 cm was created between the shoulders. Then, 0.1 ml of a solution containing C. albicans yeast (1.5 × 107 colony forming unit (CFU)) was applied to the wound. The...
Source: Comparative Clinical Pathology - March 11, 2015 Category: Pathology Source Type: research

Seborrheic keratosis with Bowenoid transformation
Abstract Seborrheic keratosis is a benign epidermal tumor but may transform to malignant lesion in sun-exposed areas of the skin. In current study, we report here a patient who presented with multiple cutaneous horns originating from seborrheic keratosis with Bowenoid transformation on the back of the shoulder as an uncommon site. An 88-year-old Iranian woman presented with a multi-conical keratotic projection lesion (7 × 8 cm) on the back of the left shoulder. A biopsy of the lesion revealed intra-epidermal neoplasm that is composed of acanthosis, hyperkeratosis, and parakeratosis. Total ...
Source: Comparative Clinical Pathology - March 4, 2015 Category: Pathology Source Type: research

Subclinical hypercorticism in patients with metabolic syndrome
Abstract Patients with metabolic syndrome who present with vascular, metabolic, or bone disorders of recent onset, are difficult to control, or are progressively deteriorating are potentially linked to cortisol excess. The aim of the study is to evaluate the prevalence of occult Cushing's syndrome (CS) in metabolic syndrome patients. Thirty women with metabolic syndrome were chosen from Endocrinology clinic El Kasr El Ani without any phonotypical features of Cushing’s syndrome. All patients were subjected to the following: full history taking with full clinical evaluation and laboratory tests including fast...
Source: Comparative Clinical Pathology - March 1, 2015 Category: Pathology Source Type: research