Metabolic adaptation after experimental clinical pregnancy toxemia in ewes
This study was performed to evaluate the effect of induction of clinical ketosis or pregnancy toxemia (PT) and subsequent effects on serum biochemical factors. Seven native pregnant ewes aged 3–4 years, 45–50 kg weight with BCS of 3.5–4 on a 0–5 scale were selected. The pregnancy of ewes was confirmed by ultrasound examination. Blood samples were harvested from the jugular vein before and after the induction of PT by food deprivation. Serum β-hydroxybutyrate (BHBA), Nonesterified fatty acids (NEFA), BUN, glucose, calcium, total protein, and cortisol concentrations were measured. Serum...
Source: Comparative Clinical Pathology - March 9, 2016 Category: Pathology Source Type: research

Herbal remedies against gastric cancer in experimental rats
Abstract Gastric cancer is the fourth most prevalent cancer worldwide, which claims more than 700,000 lives every year. However, the current chemical and surgical management of this mortal cancer remains far from successful. A recent safe approach to preventing or treating tumorigenesis has been the re-introduction of traditional herbal medicine, taking advantage of efficient, yet natural healthy products. In this review, we have focused on a number of such popular agents, mostly introduced by the Traditional Chinese medicine (TCM), which have exhibited highly significant preventive/therapeutic effects against var...
Source: Comparative Clinical Pathology - March 4, 2016 Category: Pathology Source Type: research

Erratum to: Postpartum pathology in Yankasa ewes experimentally infected with Trypanosoma evansi during pregnancy
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 3, 2016 Category: Pathology Source Type: research

Serum protein electrophoresis in Galgos
Abstract The Galgo is a member of the sighthound family and is one of the most popular breeds in Spain. Several sighthound breeds have laboratory values that are outside the reference intervals established for the general population of dogs. Serum protein electrophoresis has not been studied in Galgos and they are often interpreted as if they were Greyhounds. However, recent studies suggest that each sighthound breed presents its own specific laboratory features, and the extrapolation of the information described in Greyhounds to other sighthound breeds could lead to misdiagnoses. The aims of this study were as f...
Source: Comparative Clinical Pathology - March 1, 2016 Category: Pathology Source Type: research

Neutrophil gelatinase-associated lipocalin (NGAL) as a potential biomarker for early detection of acute renal failure
The objective of this research was to assess the ability of NGAL as a biomarker of acute renal failure by comparing the levels of serum urea and creatinine in rats with induced acute renal failure. Twelve male Wistar rats (Rattus norvegicus), 2 months of age with a body weight (BW) of 150–200 g, were allocated into two groups. Group I (n = 2), as the control group, were injected with sterile distilled water 10 mL/kg BW intramuscularly and group II (n = 10) were injected with 50 % glycerol 10 mL/kg BW intramuscular, the rats in all group had been fasting 12 h b...
Source: Comparative Clinical Pathology - March 1, 2016 Category: Pathology Source Type: research

Anesthetic effect of xylazine and benzocaine on hematological parameters and stress indicators of juvenile grass carp, Ctenopharyngodon idella (Valenciennes, 1844)
In this study, we investigated the efficacies of two anesthetics agents, xylazine and benzocaine, then compared hematological parameters and biochemical indices under optimum concentrations of two anesthetics with clove oil on juvenile grass carp, Ctenopharyngodon idella. The optimum concentrations were determined as 0.3 mg/l for xylazine and 50 mg/l for benzocaine. Significant increases in hematological parameters including white blood cell, red blood cell, and hematocrit were observed for the two anesthetics agents compared to clove oil. Plasma cortisol, glucose, and lactate concentrations increased significant...
Source: Comparative Clinical Pathology - March 1, 2016 Category: Pathology Source Type: research

The identification of bacterial flora in oral cavity of snakes
This study was carried out to determine bacterial agents existing in the oral cavity of venomous and non-venomous snakes in Kashan, Iran. Using sterile swabs, the samples were obtained in two stages from the oral cavity of 11 venomous and non-venomous snakes before feeding and 3 weeks later. Then, they were cultured on Mac Conkey blood agar mediums. Gram staining of all samples was performed. Appropriate mediums and tests were fulfilled to determine gram-positive and gram-negative bacteria. The highest rate of infection belonged to coagulase-negative Staphylococcus (34.5 %) and the lowest rate was for Pseudomonas...
Source: Comparative Clinical Pathology - March 1, 2016 Category: Pathology Source Type: research

Ontogenetic study of enteric xanthine oxidase in the chick embryos: focus on the late embryogenesis
Abstract Xanthine oxidase (XO) is a member of xanthine oxidoreductase (XOR) enzyme system that catalyses the terminal reactions of purine degradation to uric acid (UA). The synthesis of both antioxidant (i.e. UA) and numerous free radicals makes XO an important regulator of the cellular redox potential involved in normal foetal development. The aim of this study was to investigate ontogenetic changes of enteric XO activity and UA content during the last week of chick embryonic development. Fertilized eggs (n = 180) of layer-breeder (Bovans White) and broiler-breeder hens (Ross 308) were incubated at ...
Source: Comparative Clinical Pathology - February 29, 2016 Category: Pathology Source Type: research

Effect of lactation induction on milk production and composition, oxidative and antioxidant status, and biochemical variables
This study aimed to induce lactation in Lacaune sheep and to verify its influence on milk production and composition, oxidative/antioxidant profile, and biochemical variables in serum and milk. A group of ewes (group A, n = 7) was induced with estradiol (0.5 mg/kg body weight (BW)) and progesterone (1.25 mg/kg BW) on days 1 to 7, bovine somatotropin (BST; 250 mg/animal) on days 11 and 40, as well as dexamethasone (16 mg/day) on days 19, 20, and 21. For comparison, another group of pregnant ewes was used as control (group B, n = 5). Blood and milk samples were collected for bi...
Source: Comparative Clinical Pathology - February 27, 2016 Category: Pathology Source Type: research

Biochemical profiles of Indian donkey population located in six different agro-climatic zones
This study indicated that normal biochemical values of different indices for a particular population should not be used as such for disease prognosis, diagnosis and therapeutic monitoring of other donkey population belonging to other agro-climatic zone having different nutritional and managemental practices. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 27, 2016 Category: Pathology Source Type: research

Hematological and biochemical reference intervals for Bothrops asper and Crotalus simus (Serpentes: Viperidae), maintained in captivity for venom extraction
This study presents hematological and biochemical reference intervals (RIs) for two species of viper snakes (Bothrops asper and Crotalus simus), which are the first of their type particularly for these two species maintained in captivity and for extraction of venom purposes. A total of 77 snakes were used in developing the study; specifically 39 B. asper and 38 C. simus snakes. Blood samples were obtained by puncturing the caudal vein, and hematological tests were performed manually by two independent technicians. Plasma biochemistry parameters were determined by an automatized analyzer. There are hematological differences...
Source: Comparative Clinical Pathology - February 25, 2016 Category: Pathology Source Type: research

Haematological and acute-phase response of dogs with experimental skin Pseudomonas aeruginosa infection to treatment with antibiotic and parthenolide
Abstract Changes in white blood cells, leukogram patterns, the positive acute-phase protein (APP) fibrinogen and negative APPs (albumin and arylesterase) were monitored to evaluate their potential as sensitive indicators throughout the course of therapy in canine skin Pseudomonas aeruginosa infection. The study was performed on 15 male mixed-breed dogs, divided in three groups of 5 dogs each. Dogs from group A were injected subcutaneously with P. aeruginosa bacterial culture (1 × 108 CFU/mL) at a dose of 0.3 mL/kg and treated with enrofloxacin (5 mg/kg, s.c.) on post infection ho...
Source: Comparative Clinical Pathology - February 22, 2016 Category: Pathology Source Type: research

Antimicrobial activity of peptides derived from enzymatic hydrolysis of goat milk caseins
In this study, peptide fragments were produced from goat milk casein by proteolytic enzymes trypsin and ficin and combination of both enzymes, and their antimicrobial activity was investigated. After enzymatic treatments, antimicrobial activity against both Gram-positive and Gram-negative bacteria was enhanced. The hydrolysates were passed through ultrafiltration. The obtained hydrolysate by ficin (molecular weight (MW)
Source: Comparative Clinical Pathology - February 20, 2016 Category: Pathology Source Type: research

Case report: a rare right abomasal displacement in a feedlot bull calf
Abstract A Holstein male calf, less than 1 year old, was referred to the Veterinary Research and Teaching Hospital of University of Tehran (VRTH) with inappetence during the past 5 days and constipation. The first examination revealed depression and dehydration. Heart rate, respiratory rate, and rectal temperature were 107 bpm, 24 rpm, and 38.5 °C, respectively. Dehydration and hyperemia were observed in the mucus membranes. The feces were soft and scant with a foul odor. The contraction of rumen was weak, twice per minute. A clear tympanic and splashing sound was heard on the middle third of the right side in...
Source: Comparative Clinical Pathology - February 19, 2016 Category: Pathology Source Type: research

Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone
Abstract Glyphosate-based herbicides are extensively used for weed control all over the world. Thus, it is important to investigate the putative toxic effects of these commercial formulations which contain not only glyphosate but also different adjuvants. 3T3-L1 fibroblasts are a useful tool in the study of adipocyte differentiation. We have previously reported that a commercial glyphosate formulation inhibits proliferation and differentiation in these cells. In the present investigation, we further evaluated the effect of different commercial glyphosate-based herbicides on 3T3-L1 fibroblast proliferation and diff...
Source: Comparative Clinical Pathology - February 19, 2016 Category: Pathology Source Type: research

Postpartum pathology in Yankasa ewes experimentally infected with Trypanosoma evansi during pregnancy
Abstract The study was designed to determine the pathological changes in postpartum Yankasa ewes experimentally infected with Trypanosoma evansi (T. evansi) during pregnancy. Thirteen pregnant Yankasa ewes were divided into two groups comprising six ewes in group A and seven ewes in group B. Ewes in group A served as the uninfected control while ewes in group B were infected with approximately 1.0 × 106 of T. evansi per ewe through the jugular vein at the second trimester of pregnancy. They were closely monitored for trypanosomosis from the time of infection up till parturition. One ewe from ea...
Source: Comparative Clinical Pathology - February 17, 2016 Category: Pathology Source Type: research

Increased in cyclooxygenase—2 immunoreactivity and DNA damage in hippocampus of rats infected by Trypanosoma evansi
Abstract Cyclooxygenase-2 (COX-2) is one of the two isoforms of COX producing prostaglandins derived from arachidonic acid. COX-2 is the inducible isoform expressed in many cell types in response to cytokines and pro-inflammatory molecules. In brain, COX-2 is frequently associated with pro-inflammatory activities, and its increase is related to neurodegenerative processes in acute and chronic diseases. Thus, the aim of this study was to evaluate the COX-2 immunoreactivity and the frequency damage caused by T. evansi infection on days 5 and 15 post-infection (PI) in hippocampus cells, as well as the relationship wi...
Source: Comparative Clinical Pathology - February 17, 2016 Category: Pathology Source Type: research

Sensitivity and specificity of a latex agglutination test for detection of calf enterotoxigenic Escherichia coli isolates in comparison with PCR
Abstract Latex agglutination test is a very fast, accurate, and specific method that is used to diagnose antigens and antibodies which can be carried out in any place without need for any specific devices. In the present study, a single chain (scFv) recombinant monoclonal anti-K99 antibody was used in designing and producing a latex agglutination kit for the detection of enterotoxigenic Escherichia coli of calves colibacillosis. The purpose of this research was to primarily evaluate the sensitivity and specificity of the mentioned latex agglutination kit in the detection of the considered bacteria and to compare i...
Source: Comparative Clinical Pathology - February 15, 2016 Category: Pathology Source Type: research

Chemical and electrical approaches to anesthesia of Ship sturgeon, Acipenser nudiventris : induction and recovery, physiological response to anesthesia
In this study, we investigated the induction and recovery times and physiological response of juvenile Ship sturgeon, Acipenser nudiventris anesthetized by CO2 (bar), clove powder (mg/L), and available electric shock (V). The shortest and longest induction time was for electric shock and clove powder, respectively. By contrast, the shortest and longest recovery time took place for electric shock and clove powder, respectively. Cortisol and glucose levels increased in 1 and 6 h after anesthesia. Changes in plasma osmolality and hematological indices were less changed among the anesthesia treatments. Results demonstrate...
Source: Comparative Clinical Pathology - February 10, 2016 Category: Pathology Source Type: research

Congenital naso-orbital meningocele in a lamb: a case report
Abstract The subject of this report is a rare case of naso-orbital meningocele concurrent with right cerebral hemisphere hydranencephaly and agenesis of corpus callosum in a newborn lamb. Our necropsy findings revealed that the bulging sac containing CSF extends into the skull bony and dural defects, through which the cyst protrudes into the orbital and nasal cavities. Also, histological and immunohistochemical demonstrations of the cyst wall showed that the inner layer is composed of meningeal components. These findings support the diagnosis of a naso-orbital meningocele. This case is an unusual presentation of a...
Source: Comparative Clinical Pathology - February 2, 2016 Category: Pathology Source Type: research

Idiopathic pulmonary fibrosis in a captive lion ( Panthera leo ): case report
This report would further improve the screening of wildlife health. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 2, 2016 Category: Pathology Source Type: research

The association between G-197A gene polymorphism of IL-17A with changes in protein interaction of IL-17A, levels of urinary IL-17, and degree of lupus nephritis abnormality
In conclusion, the levels of uIL-17 in LN patients were significantly higher than those in the control group, but G-197A gene polymorphism of IL-17A did not cause varying levels of IL-17 and did not influence the degree of renal abnormalities in LN. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 2, 2016 Category: Pathology Source Type: research

Evaluation of the changes of P1NP and CTX in synovial fluid and blood serum of dogs with experimental osteoarthritis
Abstract Osteoarthritis is a degenerative and reparative process occurring in synovial joints. In the present study, collagen type I C-telopeptide (CTX) and procollagen type I N-terminal propeptide (P1NP) were measured in blood serum and synovial fluid of four cross-breed dogs with experimental CCL rupture. These biomarkers were selected as bone turnover biomarkers. The markers were measured on days 0, 14, 28, 90, and 180 post surgery. Statistical analysis showed significant increase in P1NP in both serum and synovial fluid. The CTX showed a significant increase in serum and a non significant increase in synovial ...
Source: Comparative Clinical Pathology - January 28, 2016 Category: Pathology Source Type: research

Safety evaluation of Acalypha wilkesiana in albino rats and BHK-21 cell line
We examined the phytochemical profile, cytotoxic effects on baby hamster kidney cell line (BHK-21), and oral subacute toxicity of A. wilkesiana leaf decoction in rats. Rats were given 0, 300, 600, and 1200 mg/kg body weight of A. wilkesiana leaf extract, daily, orally for 14 days. The phytochemical profile showed the presence of flavonoids, saponin, cardiac glycosides, and tannins. It caused apoptosis in BHK-21 cell line at concentrations of 0, 25, 50, 100, and 200 μg/ml. There was significant increase in the levels of serum AST, ALT, creatinine, urea, Na+, K+, and Cl− levels in all the test groups ...
Source: Comparative Clinical Pathology - January 26, 2016 Category: Pathology Source Type: research

Expression of oestrogen receptor α in the endometrium of porcine reproductive and respiratory syndrome virus-infected gilts
Abstract Porcine reproductive and respiratory syndrome (PRRS) virus infection often lead to infertility in gilts and sows. Nevertheless, the impact of PRRS virus on the endometrial function has not been fully elucidated. The present study aimed to determine the effect of PRRS virus infection on the expression of oestrogen receptor (ER) α in the endometrium of gilts. Uterine tissues from 54 gilts were classified into two groups according to PRRS virus detection using immunohistochemistry (26 positive and 28 negative samples). The reproductive status was classified as prepubertal, luteal and follicular phases....
Source: Comparative Clinical Pathology - January 25, 2016 Category: Pathology Source Type: research

Evaluation of selected biochemical parameters and hepatic enzymes activity in serum of cattle naturally infected with Dicrocoelium dendriticum in Semnan Province, Iran
Abstract The present study was conducted to investigate the effect of Dicrocoelium dendriticum on some biochemical parameters and hepatic enzymes activity in serum of cattle during the subclinical phase (asymptomatic) of the disease. Blood samples and liver of 33 indigenous cattle of Mazandarani breed, slaughtered in the period from September to November 2013, were collected. Based on parasitological findings in the liver, all animals were divided into two groups: control (n = 16) and infected group (n = 17). Biochemical analysis of tested parameters showed a significant elevation (P&th...
Source: Comparative Clinical Pathology - January 21, 2016 Category: Pathology Source Type: research

Impact of cellular CD71 (transferrin receptor 1) expression in Egyptian acute leukemia: correlation with clinicopathologic features
In conclusion, CD71 is overexpressed in acute leukemia; it predicts adverse clinical outcome in ALL. In addition, CD71 antagonism could be a possible therapeutic target in acute leukemia. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 20, 2016 Category: Pathology Source Type: research

Therapeutic management of epistaxis due to ehrlichiosis in a dog
Abstract A Labrador bitch was diagnosed that it was suffering with clinical ehrlichiosis. Dog exhibited epistaxis, tachycardia and hyperpnoea. Dog showed signs of anaemia, increased alanine transaminase and aspartate transaminase levels. Present case was successfully treated with oxytetracycline and doxycycline along with supportive therapy. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 20, 2016 Category: Pathology Source Type: research

Antibodies against Toxoplasma gondii in bats ( Desmodus rotundus ) captured near caves in cities from western region of Santa Catarina State, Brazil
This study aimed to investigate the presence of antibodies against T. gondii in the serum of 50 vampire bats (Desmodus rotundus) from the western region of Santa Catarina State, Brazil, using the modified agglutination test (MAT), in a dilution of 1:25. The results showed 10 % (5/50) are seropositive for T. gondii, showing that bats from that region had contact with T. gondii and are probably infected. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 15, 2016 Category: Pathology Source Type: research

The seasonal occurrence of Well’s syndrome
Abstract Well’s syndrome, in many circumstances, is determined by intensive eosinophilic infiltration into dermal layer with the clinical presentation of erythematous and edematous plaques which can easily be remitted by current corticosteroid therapies. Up to now, different etiologies have been proposed governing Well’s pathophysiological procedure. Of note, no evidence of seasonal association has been already reported. A 70-years-old afebrile male presented with a chief complaint of 30-years history of cutaneous manifestations along with non-ulcerated edematous, erythematous plaques and patches, and ...
Source: Comparative Clinical Pathology - January 6, 2016 Category: Pathology Source Type: research

Recurrent Malassezia dermatitis due to hypothyroidism in a dog and its management
Abstract Malassezia pachydermatis is the most common causative organism for development of Malassezia dermatitis in dogs. An adult pug was presented to the clinic with chronic skin abnormalities, rancid odour from the body and pruritus. Lesion distribution was noticed on the ventral chin, neck, abdomen and inner surface of the legs. Skin scrapings, hair plucks and tape impression smears were examined, and footprint-shaped Malassezia organisms were identified in impression smears. Blood was collected to diagnose underlying factor for development of chronic recurrent Malassezia dermatitis. Up on blood...
Source: Comparative Clinical Pathology - December 28, 2015 Category: Pathology Source Type: research

Vacuolar myopathic lesion in a Holstein-Friesian dairy cow affected with bovine dilated cardiomyopathy: a preliminary report
Abstract Occurrence of vacuolar myopathy caused by factors other than inherited glycogen storage condition is uncommon in domestic animals. This preliminary report describes the detection of a vacuolar myopathic lesion in a 13-year-old Holstein- Friesian dairy cow which showed an 8-day history of cardiac disease. Gross inspection after slaughter revealed edema in a variety of tissues, dilatation and hypertrophy of the cardiac ventricles, and hepatic congestion. Histopathology revealed degenerative myocardial lesions and passive hepatic congestion, which were identical to those previously documented in cases of bov...
Source: Comparative Clinical Pathology - December 22, 2015 Category: Pathology Source Type: research

Trypanosoma vivax infection in goat in west of Santa Catarina state, Brazil
Abstract The aim of this study was to report the Trypanosoma vivax in goats from a property located in the west of the state of Santa Catarina, Brazil. The identification of the parasite occurred at random, that is, it was displayed a similar morphological trypomastigote form in the blood smear of an animal in the property. Subsequently, in blood samples collected from five goats on the same day, it was confirmed by polymerase chain reaction (PCR) the presence of the parasite in three of these animals. Being that by chance, consistent clinical signs related to trypanosomosis in these animals were not observed. The...
Source: Comparative Clinical Pathology - December 21, 2015 Category: Pathology Source Type: research

Clinical evaluation of the effects of supplementation with curcuminoids on serum fetuin-B concentrations in obese subjects
Abstract Fetuin-B is an emerging negative acute phase protein, and its expression is inversely associated with inflammation. The present study aimed to assess the impact of supplementation with curcuminoids, as naturally occurring polyphenols with anti-inflammatory properties, on circulating concentrations of fetuin-B. In this randomized double-blind trial, 30 obese dyslipidemic subjects received curcuminoids (1 g/day) and matching placebo. The trial had a 2 × 2 crossover design, in which treatment duration with either of the allocations was 4 weeks, intermitted by a 2-week washout pe...
Source: Comparative Clinical Pathology - December 21, 2015 Category: Pathology Source Type: research

Haematological and serum biochemical reference values for captive blue-fronted amazon parrot
This study aimed to determine haematology and biochemistry reference values for A. aestiva to assist veterinarian and conservation programmes in the care of this species. Sampling was conducted in April 2014; a total of 32 birds were manually restrained, and their blood samples were collected and divided into two aliquots. Haematological parameters evaluated included red blood cell (RBC) count, white blood cell (WBC) count, differential leukocyte count, haematocrit, thrombocyte count, haemoglobin concentration and RBC indexes. The biochemical parameters measured were alkaline phosphatase, alanine aminotransferase, gamma gl...
Source: Comparative Clinical Pathology - December 18, 2015 Category: Pathology Source Type: research

A mini review of histological and genetic oral lesions in adult and elderly patients referred to Shiraz Dentistry School
This article is a mini review of some common oral lesions especially in adults and elderly patients who attended Shiraz Dentistry School, Iran, and is designed to help all dental practitioners to be more ready to deal with these lesions. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - December 15, 2015 Category: Pathology Source Type: research

Novel data for free-living saffron finch ( Sicalis flaveola ): reference hematological value and body mass
Abstract The Sicalis flaveola specie is also known as saffron finch. It is known to be in the illegally traded and mostly to be used as pets. Although the saffron finch is not listed as threatened species, the hunting, destruction of habit, and introduction of hybrid and exotic specimens to the natural environment can put the specie in risk, and they have been already cited in some regions to be found rarely. Due to the lack of hematological data for the specie S. flaveola, this study aimed to describe the hematological reference values and biometric data (weight) for the specie. Thirty-eight saffron finches where...
Source: Comparative Clinical Pathology - December 14, 2015 Category: Pathology Source Type: research

Hematological reference values of healthy adult Sangsari sheep (Iranian fat-tailed sheep) estimated by Reference Value Advisor
In this study, we used a total 63 healthy Sangsari sheep at the same physiological status for both sex with two ages (2–4 years) from June to July 2014. Hematological parameters were measured by routine methods, and finally, reference interval (RI) was determined by the robust method and Box-Cox transformation (Reference Value Advisor). The RI of hematological parameters obtained in this study includes the following: hematocrit (Hct) 14.3–35.3 %; Hb 5.4–12.52 gr/dl; mean corpuscular volume (MCV) 26.2–36.3 fl; mean corpuscular hemoglobin (MCH) 8.6–12.3 pg; mean corpuscu...
Source: Comparative Clinical Pathology - December 11, 2015 Category: Pathology Source Type: research

Alternative feedstuffs and their effects on blood chemistry and haematology of rabbits and chickens: a review
Abstract The demand for meat in developing countries is on the increase, and this trend is expected to continue over the years. The ability of poultry and rabbit to meet the animal protein demand in developing countries cannot be overemphasized. However, the main constraint limiting effective and efficient use of these choice animals is the high cost of traditional feed ingredients such as maize, soybean meal, fish meal and groundnut cake which are in strict competition with man’s dietary need. However, it is also becoming clear that the requirements for the traditional feed ingredients cannot be met, even a...
Source: Comparative Clinical Pathology - December 8, 2015 Category: Pathology Source Type: research

Horner’s syndrome and unilateral facial paresis in a goat: a case report
Abstract A 4-year-old, female Pakistani goat was referred to the veterinary teaching hospital for first pregnancy check up. At the time of presentation, the case was apparently healthy. Incidentally, the clinicians noticed the abnormality in the left eye. A precise ophthalmic examination revealed ptosis, entropion, nictitating membrane prolapse, enophthalmos, and miosis in the left eye, whereas the eyelids and pupil were normal on the right side. The left eye lacked palpebral reflex, and the menace response seemed to be decreased. Pupillary light reflex was present in both eyes. The eyes did not show any evidence ...
Source: Comparative Clinical Pathology - December 7, 2015 Category: Pathology Source Type: research

Duration of blood plasma cortisol suppression after a low-dose dexamethasone suppression test in dogs
Abstract The aim of this study was to determine the duration of the suppressive effect on the hypothalamus–pituitary–adrenocortical axis as measured by plasma cortisol concentration after injection of a low dosage of dexamethasone (0.01 mg/kg BW) used within the low-dose dexamethasone suppression test (LDDST). A LDDST was performed on ten clinically healthy dogs with unremarkable haematological and plasma biochemical profiles, abdominal X-ray, abdominal ultrasonography and fine needle aspiration cytology of the liver. Two of them were excluded from the analysis. Blood samples were collected at bas...
Source: Comparative Clinical Pathology - November 27, 2015 Category: Pathology Source Type: research

Expression of metallothionein protein relating to proliferative cell index in malignant feline mammary tumors using high throughput tissue microarray technique
Abstract The present study aimed to examine the relationship between proliferation markers by detecting Ki-67 and proliferative cell nuclear antigen (PCNA) indices, and metallothionein expression, in different histological groups and nuclear grades of malignant feline mammary tumors by using immunohistochemistry and tissue microarray technique. Seventy feline mammary carcinoma (FMC) biopsy specimens were collected to perform tissue microarray (TMA) slide. The immunohistochemical staining was performed on the TMA slides against pancytokeratin (CK19), PCNA, Ki-67 clone MIB, and metallothionein 1 (MT-1) antibodies. T...
Source: Comparative Clinical Pathology - November 27, 2015 Category: Pathology Source Type: research

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients
In this study, we have discussed T cell receptor (TCR) gene rearrangements in ALL. As leukemia cell proliferation originates from a unique clone, clone-specific rearrangement can be very helpful in diagnosis and detection of remaining malignant cells from among normal cells for minimal residual disease. On the other hand, each of the TCR genes is associated with translocation in ALL types, and the result of gene rearrangements can be used as prognosis markers. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 23, 2015 Category: Pathology Source Type: research

Renal structure and its concentrating ability in the Persian squirrel ( Sciurus anomalus )
Abstract The present study was conducted to investigate the morphological characteristics of the kidney and its urine concentrating ability in the Persian squirrel. The kidneys of five male Persian squirrel which suffered from diseases unrelated to the urogenital system were used for morphometric measurements and biochemical analysis of blood and urine under their owner’s permission. There was no statistical significance found between the linear measurements (length, width, and thickness) between the right and left kidneys (P > 0.05). Also, no statistically significant difference existed bet...
Source: Comparative Clinical Pathology - November 23, 2015 Category: Pathology Source Type: research

The case report on cutaneous myxoma or invasive myxomatosis in Blackfin sea catfish ( Arius jella Day, 1877)
Abstract The presence of cutaneous myxoma is reported in a Blackfin sea catfish (Arius jella) from Vellar estuary, Parangipettai, southeast coast of India. The gross morphology revealed fleshy with gelatinous distended tumourous growths on both sides of the fish attached to the caudal peduncle region. Tumour overlying the epidermis was ulcerated, hyperemic and hyperchromatic. Histologically, the tumourous growth was characterized by bland spindloid cells embedded in an abundant myxoid extracellular matrix. The tumour masses were present within the dermis and covered with a thin attenuated layer of epidermis. Neopl...
Source: Comparative Clinical Pathology - November 19, 2015 Category: Pathology Source Type: research

Ectopic cross ureter with uterus didelphys in a lamb
Abstract The development and incorporation of the ureteric bud into the developing urinary bladder is a complex process, which is simultaneously influenced by events occurring in the development of other systems in the perineum. Knowledge of the embryology of the ureteric bud and associated Wolffian duct is the key to understand the development of the entire genitourinary system. In this article, we have reported and discussed a 1-day dead lamb with congenital ectopic ureter, open urachal sinus and uterus didelphys. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - November 17, 2015 Category: Pathology Source Type: research

Hepatocellular carcinoma in a cow: detection of tumor-infiltrating T lymphocytes implicated in anti-tumor immune response and partial spontaneous tumor regression
This report describes the morphological finding in a 12-year-old Holstein-Friesian dairy cow with spontaneous hepatocellular carcinoma. The cow that showed anorexia, emaciation, depression, and sluggish movement was culled and exhibited multiple tumor nodules (
Source: Comparative Clinical Pathology - November 17, 2015 Category: Pathology Source Type: research

Survivin and SIRT1: can be two prognostic factors in chronic myeloid leukemia?
In this study, we measured the expression of SVV and SIRT1 in 18 patients with typical Ph-positive chronic myeloid leukemia in chronic phases of the disease as well as in 18 matched healthy donors. SVV, SIRT1, and BCR/ABL expression were determined by quantitative-real-time polymerase chain reaction (Q-RT-PCR). HPRT was used as a reference gene to calculate SVV/HPRT and SIRT1/HPRT ratio, and ABL was used as an internal control for BCR/ABL expression. SVV was between 0.00 and 2.39-fold (median 0.204) and SIRT1 was between 0.00 and 33.32-fold (median 0.186). In our study, both SVV and SIRT1 were lower in patients than in the...
Source: Comparative Clinical Pathology - November 16, 2015 Category: Pathology Source Type: research

Culture-dependent assessment of bacterial diversity from human milk with lactational mastitis
The objective of this study was to explore culturable bacterial diversity present in the breast milk of mastitis-suffering women by 16S rDNA sequencing and evaluate its antibiotic susceptibility. The culturable bacteria present in the breast milk of 32 women with lactational mastitis were studied. Thirty-seven bacterial isolates comprised of distinct colony characteristics were checked for antibiotic susceptibility and identified by 16S rDNA sequencing. Sequencing of 16S rDNA and BLAST analysis revealed the presence of 17 genera and 30 different species indicating highly diverse community in mastitis milk. The Proteobacter...
Source: Comparative Clinical Pathology - November 16, 2015 Category: Pathology Source Type: research

Evaluation of clinical, hematological, and biochemical changes following autologous blood transfusion in goats
Abstract Blood transfusion is an important remedy to manage animals with severe anemia. The risk of pathogen transmission can be reduced using autologous blood transfusion. Due to the lack of studies related to the use of autologous blood transfusions in goats, this study was conducted to evaluate the clinical, hematological, and biochemical changes following blood collection and reinfusion in goats. Fifteen milliliters per kilogram of whole blood was collected and reinfused after 7 days. The heart rate and respiratory rate were increased following blood collection and decreased to normal values within 48 and...
Source: Comparative Clinical Pathology - November 16, 2015 Category: Pathology Source Type: research