Morphometric evaluation of gross pulmonary indices in the Red Sokoto goats for pulmonary consolidation assessment
In conclusion, this study established the morphometric and percentage contribution of each lung lobe in relation to the age and sex of the RSG. These parameters will aid diagnosis of lung disease and assessment of pulmonary consolidation at postmortem in a poor resource setting. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 13, 2019 Category: Pathology Source Type: research

Toxicological evaluation of aqueous extract of different varieties of guava ( Psidium guajava Linn) leaves
AbstractGuava (Psidium guajava Linn) is a common fruit tree in Nigeria with different species; the leaves, fruits, and the bark are often used in folklore for different medicinal purposes. However, there is a dearth of information on safety assessment of the leaves extract of guava from different species. These studies conducted toxicological evaluation of white, red, and pink guava leaves extract in rats for 14  days. Oral administration of 50, 500, and 5000 mg/kg BWT was carried out; they were observed for toxicity signs hourly for the first 6 h after administration, and every day of the experimental perio...
Source: Comparative Clinical Pathology - June 13, 2019 Category: Pathology Source Type: research

Molecular detection of Theileria ovis (Apicomplexa: Theileriidae), Anaplasma ovis (Rickettsiales: Anaplasmataceae), and Mycoplasma sp. (Tenericutes: Mycoplasmataceae) from sheep blood in western Iran
This study is an investigation on tick-borne pathogens of domestic sheep, and aMycoplasma agent is reported for the first time in Iran. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - June 13, 2019 Category: Pathology Source Type: research

DGGR-lipase for effective diagnosis of pancreatitis in horses
AbstractEquine pancreatitis has no reliable ante-mortem test and is considered rare. The 1,2-o-dilauryl-rac-glycero-3-glutarate-(6 ′-methylresorufin) ester (DGGR) lipase assay is specific for pancreatic lipase (PL). It was developed for humans and validated in dogs, cats and rats as specific for pancreatitis diagnosis. We used a novel validation strategy to confirm this tissue specificity extended to horses and determine refe rence ranges and pancreatitis prevalence. One hundred thirty-nine horses were used. Biopsies were collected from 3 healthy cadavers and venous blood from 109 clinical cases and 27 training raceh...
Source: Comparative Clinical Pathology - June 13, 2019 Category: Pathology Source Type: research

Concurrent infection of Leptospira icterohaemorrhagiae , Hepatozoon canis and Anaplasma phagocytophilum in a Labrador Retriever dog and its therapeutic management
In this report, concurrent infection ofLeptospira interrogans serovar icterohaemorrhagiae,Hepatozoon canis andAnaplasma phagocytophilum in a Labrador Retriever dog and its successful therapeutic management have been described. A 3-year-old female dog was presented with a history of anorexia, fever, lethargy, weakness, vomiting, black-coloured faeces and yellowish urine. Clinical and laboratory examination revealed pyrexia, icteric mucous membranes, hepatomegaly, splenomegaly, anaemia, leukocytosis, thrombocytopaenia, hypoalbuminemia and hyperbilirubinemia.Hepatozoon canis andAnaplasma phagocytophilum infections were confir...
Source: Comparative Clinical Pathology - June 11, 2019 Category: Pathology Source Type: research

Biomarkers of oxidative stress in Clarias gariepinus for assessing toxicological effects of heavy metal pollution of Abereke river in southwest Nigeria
AbstractWater pollution indices and oxidative stress biomarkers in African sharp-toothed catfish,Clarias gariepinus were used to evaluate the health status of Abereke River on the Ilaje coastal zone of Ondo ’s Niger Delta region. Muscle and liver tissues from fishes harvested from the river and from a clean fish farm were comparatively subjected to heavy metal status and redox stress biomarker analyses. Water samples from the river and fish farm were also subjected to water quality tests. Our finding s showed significant elevations in bacterial contaminants, heavy metal pollutants, and particulate matter deposits in ...
Source: Comparative Clinical Pathology - June 11, 2019 Category: Pathology Source Type: research

Pathology and bronchoalveolar lavage fluid cellular and oxidative stress changes in canine pneumonia in Nigeria
This study is on the pathology and bronchoalveolar cellular and oxidative stress changes in naturally occurring canine pneumonia in Nigeria. The dogs used were collected from cases submitted for necropsy in the institutional Department of Veterinary Pathology. Gross examination, bronchoalveolar lavage (BAL) cytology, oxidative biochemical assay of BAL fluid (BALf) supernatant and histology of sections from all the lung lobes were done using standard procedures. Grossly, 22 (31%) were normal while 48 (69%) were congested, haemorrhagic and/or pneumonic out of the 70 canine lungs examined. Microscopically, there were pulmonar...
Source: Comparative Clinical Pathology - June 7, 2019 Category: Pathology Source Type: research

Induction of immune responses by protein vaccines formulated with adjuvants against Leishmania major in vivo
AbstractWe used TSA as a principal antigen ofLeishmania major with adjuvants. The aim of this research was to assess the efficacy of protein vaccine in the presence of Montanide vis- à-vis chitosan nanoparticle. The expression of recombinant protein was confirmed with SDS (sodium dodecyl sulfate) page and Western blotting. A total of 36 BALB/c mice were divided into six groups (TSA/high chitosan, TSA/low chitosan, TSA/Montanide, high chitosan, low chitosan, and PBS groups) and subcutaneously immunized with 20 mg of vaccine at three time intervals on days 0, 14, and 28. The mice were challenged with parasites 21...
Source: Comparative Clinical Pathology - June 7, 2019 Category: Pathology Source Type: research

Impaired epididymal function of albino rats infected and treated with Trypanosoma brucei brucei and Trypanosoma congolense
AbstractThe effect of trypanosomosis (Trypanosoma brucei brucei andTrypanosoma congolense infection) and diminazene aceturate treatment on the epididymal function of male albino rats was investigated. Twenty-five adult male albino rats were used for this study. They were randomly assigned into 5 groups of 5 rats each. Group 1 was the uninfected untreated group while groups 2 and 3 were infected with 5  × 105T. congolense. Groups 4 and 5 were infected with 5  × 105T. brucei brucei. Rats in groups 2 and 4 were humanely sacrificed at the peak of parasitemia, i.e., on days 7 and 14 PI re...
Source: Comparative Clinical Pathology - June 6, 2019 Category: Pathology Source Type: research

Prevalence and pathological features of ovine lungworm infection in slaughtered animals in Nangarhar Province of Afghanistan
AbstractLungworms are considered one of the main causes of respiratory disorder in sheep worldwide that causes huge economic losses for the sheep industries. A cross-sectional study was carried out from March 2016 to April 2017 to characterize the pathological features and estimate the prevalence of lungworm infection in slaughtered sheep in Nangarhar Province of Afghanistan. Multiple emphysematic areas, white and consolidated spots and different sizes of nodules, and white –creamy worms in frothy material within the trachea and bronchi were observed grossly in affected lungs. In histopathological examination, bronch...
Source: Comparative Clinical Pathology - June 5, 2019 Category: Pathology Source Type: research

Toxicity effects of mercury and silver nanoparticles on common carp ( Cyprinus carpio )
In conclusion, the results indicated that the silver nanoparticles enhanced the toxicity and uptake of mercury in tissues of carp. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 29, 2019 Category: Pathology Source Type: research

Grafting with double-layered polypropylene mesh for gap healing of mid-metacarpal superficial digital flexor tendon in Equus asinus : clinical and pathological evaluations
AbstractTendon injury is one of the most common causes of wastage in equine. The superficial digital flexor tendon (SDFT) gets affected the most, especially in the mid-metacarpal region. Several studies have focused on the grafting of severed SDFT, but no ideal grafting material has been approved for clinical application. The present study aimed to evaluate the grafting of the mid-metacarpal SDFT in donkeys with a double-layered polypropylene mesh clinically, ultrasonically, macro/microscopically. Clinically healthy adult donkeys (n = 24) were divided randomly and equally into two groups: control (C) and stud...
Source: Comparative Clinical Pathology - May 29, 2019 Category: Pathology Source Type: research

The morphological characteristics of the Meckel ’s diverticulum of pre-hatch and post-hatch broiler chicken
In conclusion, the present study has provided clear structural evidence which shows that intense cellular proliferation occurs in the MD of broiler chicken between embryonic days 14 and 19. This organ may play signif icant roles in immunological surveillance of the gut, and such functions may peak on post-hatch day 35. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 28, 2019 Category: Pathology Source Type: research

Some hematological and biochemical reference values of the thoroughbred Appaloosa horse breeds reared in Kyrgyzstan
AbstractThe aim of this study was to determine the normal hematological and biochemical parameters of the Thoroughbred Appaloosa horse breeds which reared in Tong and Iss ık-Kul regions of Kyrgyzstan. Fifty-six (n = 56) healthy horse breeds in different ages (0–12 months and 1–5 years) and gender (n = 29 male andn = 27 female) were clinically examined and blood samples were taken. Red blood cell (RBC) count, white blood cell (WBC) count, the count (#); the percentage (%) of the lymphocytes (lymph), monocytes (mid), granulocytes (gran); and the values of hemog...
Source: Comparative Clinical Pathology - May 28, 2019 Category: Pathology Source Type: research

Comparative study on the effects of heated brass bar and scald methods in experimental skin burn in rat
This study highlights the benefits of scald method in comparison with the heated brass bar. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 26, 2019 Category: Pathology Source Type: research

Effects of asiatic acid, an active constituent in Centella asiatica (L.): restorative perspectives of streptozotocin-nicotinamide induced changes on lipid profile and lipid metabolic enzymes in diabetic rats
AbstractThe present study was aimed to evaluate the restorative efficacy of asiatic acid (AA), an active constituent inCentella asiatica (L.) in streptozotocin (STZ)-nicotinamide (NAD)-induced diabetic rats. Male Wistar albino rats were made diabetic with STZ-NAD administration and assessed the effect of AA (20, 30, and 40  mg/kg body weight per day, for 45 days) on body weight, glucose, insulin, insulin resistance, lipid metabolic parameters in plasma and tissue (liver and kidney), and histopathology of liver in control and experimental diabetic rats. A significant (p 
Source: Comparative Clinical Pathology - May 24, 2019 Category: Pathology Source Type: research

Publishing quandaries for scientists from developing countries
AbstractResearchers ought to publish their research articles in a scientific journal to get their work recognized. Publication of scientific research output is crucial for the dissemination of scientific information. But, they are confronted with variety of challenges in publishing their findings, even more when they are researchers from developing countries. I therefore in this paper wish to draw attention to the challenges faced by scientist from developing countries to document their work keeping my focus on one of the major criteria set by journals, i.e., inclusion of molecular tests and/or novelty which are in particu...
Source: Comparative Clinical Pathology - May 23, 2019 Category: Pathology Source Type: research

Effect of radio frequency electromagnetic field from mobile phones ’ base station antennas on maturation of rat erythrocytes
AbstractThe biological effects of radiation emitted by mobile telephone base station antennas are still controversial. The present study was designed to evaluate the effects of radio frequency electromagnetic field frequency range of 1200 –1400 MHz, from a mobile phone base station on erythropoietic cells in the rat bone marrow. Out of a total of 48 rats, 24 were exposed for 2 h each day for 7, 14, 30, and 60 days to electromagnetic radiation at specific absorption rate (SAR) levels 1.165 W/kg. Electromagnetic field–exposed r ats were compared with sham-exposed control rats. The animals of t...
Source: Comparative Clinical Pathology - May 21, 2019 Category: Pathology Source Type: research

Clinical and laboratory investigations of poor performing anemic Egyptian horses infested with Theileria equi
This study aimed to evaluate hematology, biochemical constituents, acid-base balance, and blood gases in poor-performing anemic working horses. Thirty Egyptian Balady horses divided into two groups (15 healthy horses as a control group and 15 poor-performing anemic workin g horses). Blood samples were taken from each animal and divided into three portions, the first portion collected on EDTA tubes for hematology examination, the second portion collected on Heparin and sent immediately for blood gases and acid-base estimation, and the last portion collected on plain t ubes for serum separation. Giemsa-stained blood films we...
Source: Comparative Clinical Pathology - May 20, 2019 Category: Pathology Source Type: research

Biochemical changes in selected tissues of normal albino rats following treatment with 1,3-bis [(furan-2-yl) methylene] urea
AbstractThis research investigated the effects of 1,3-bis(furan-2-yl)methylene) urea (BFMU), a synthesized imine compound with potential biological activities, on selected biochemical parameters of albino rats. The compound at varied concentrations (12.5, 25, and 50  mg/kg body weight) was administered intraperitoneally to albino rats for 28 days. At the end of the experiment, activities of enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) were assayed. Also, activities o f antioxidants including catalase (CAT), superoxide di...
Source: Comparative Clinical Pathology - May 20, 2019 Category: Pathology Source Type: research

Activity of Adansonia digitata on biochemical and hematological changes in established Plasmodium berghei -infected mice
This study evaluated the efficacy ofAdansonia digitata stem bark extract on hematological and renal changes in establishedPlasmodium berghei infection in mice. Albino wistar mice were used in the study. The mice were transfected intraperitonially with an inoculum size of l  × 107 of chloroquine susceptible strain ofPlasmodium berghei-infected erythrocytes. Percentage chemosupression and packed cell volume (PCV) were determined. The antiplasmodium effects of the extracts were investigated by measuring the hemoglobin (Hb) content, white blood cell (WBC) count, and cell differentials. Severity of malaria w...
Source: Comparative Clinical Pathology - May 18, 2019 Category: Pathology Source Type: research

Serum biochemical profiling in different varieties of Japanese quail, Coturnix coturnix japonica (Temminck and Schlegel, 1849)
AbstractA good quantum of reports is in record on serum biochemical analysis of Japanese quail. But the serum biochemical parameter of different varieties of healthy Japanese quail is poorly understood, and reference values are sparsely available. The investigation focuses to assess serum biochemical profiles of three different varieties of Japanese quail, i.e. brown-feathered, grey-feathered, and white-feathered at different age groups with sexual dimorphism. The five parameters taken for the analyses include glucose, cholesterol, total protein, albumin, and globulin. Two millilitre of blood was collected from the ulnar v...
Source: Comparative Clinical Pathology - May 18, 2019 Category: Pathology Source Type: research

Brilliant blue G protects against brain and liver tissue damage during systemic endotoxemia in rats treated with lipopolysaccharide
In this study, the effect of brilliant blue G on serum oxidative stress and brain and liver tissue damage was studied in rats with lipopolysaccharide (LPS)-induced systemic inflammation. Rats were treated with intraperitoneal (i.p.) injection ofEscherichia coli LPS (300  μg/kg) alone or together with brilliant blue G at 5 or 10 mg/kg and euthanized 4 h thereafter. Serum malondialdehyde, nitric oxide, paraoxonase 1 (PON-1) activity, cholinesterase activity, and glucose were determined. In addition, brain and liver histopathology, and caspase-3 and glial fibrilla ry acidic protein (GFAP) immunostaining were...
Source: Comparative Clinical Pathology - May 18, 2019 Category: Pathology Source Type: research

Effects of aqueous leaf extracts of Loranthus micranthus Linn. on hematological profile of albino rats infected with Trypanosoma brucei brucei
AbstractsAfrican trypanosomiasis has continued to threaten human health and economical development (Kuzoe Acta Trop 54:153 –162,1993; WHO J Tsetse Tryp Info Quart 3:4 –9,2000). It is caused by parasitic protozoa of the genusTrypanosoma (Adeiza et al. J Med Plant Res 4(17):1770 –1777,2010), and the distribution corresponds with that of tsetse flies. Effects of aqueous leaf extracts ofLoranthus micranthus on hematological parameters of albino rats infected withTrypanosoma brucei brucei were investigated for 28  days using standard methods. The HB, PCV, RBC, and its indices (MCH, MCV, and MCHC) of the i...
Source: Comparative Clinical Pathology - May 14, 2019 Category: Pathology Source Type: research

Editorial
(Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 14, 2019 Category: Pathology Source Type: research

Cytotoxicity, antioxidant, anti-inflammatory activity, and GC-MS analysis of Egyptian propolis
AbstractNatural products have a great contribution to folk medicine because it is rich in various and unique compounds. Propolis is considered one of the most important natural products due to their different medical applications. In this work, the antioxidant, anti-inflammatory, and cytotoxicity of propolis to cancer cell lines were investigated which was obtained from Bee Research Department, Plant Protection Research Institute and Agricultural Research Center, Egypt. The antioxidant activity was evaluated by DPPH assay. The propolis concentration of 1280  μg/ml achieved the highest value (91.42%) of the antioxid...
Source: Comparative Clinical Pathology - May 11, 2019 Category: Pathology Source Type: research

Clinico-pathological responses in reproductive system and its associated lymph nodes of bucks challenged with Corynebacterium pseudotuberculosis and its mycolic acid extract
In conclusion, this research revealed thatC. pseudotuberculosis has a negative effect on reproductive performances of bucks and affects the clinical responses and abscess formation. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 10, 2019 Category: Pathology Source Type: research

Haematological, serum biochemical and histopathological changes in hepatitis E virus seropositive pigs in Ibadan, Nigeria
This study evaluated the haematological, serum biochemical and histopathological changes in HEV seropositive pigs. A total of 120 pigs were sourced from the Bodija Municipal abattoir, Ibadan, Nigeria, for this study. The haematology and serum biochemistry of the sampled pigs seropositive to HEV antibodies using the ID Screen ® Hepatitis E Indirect Multi-species ELISA biwell kit were analysed. Liver and spleen samples were also taken for histopathological examination and lesion scoring. HEV seropositivity was reported in 57.5% (69/120) of the pigs and associated with a significant (p 
Source: Comparative Clinical Pathology - May 10, 2019 Category: Pathology Source Type: research

A study of clinical and pathological findings in an ovine experimental theileriosis
AbstractThe malignant ovine theileriosis due toTheileria lestoquardi is highly pathogenic in sheep. During an experimental study, clinical and pathological findings were investigated in sheep infected withT. lestoquardi. The common clinical signs were high fever, emaciation, enlarged lymph nodes, anemia, icterus, nasal discharge, cough, severe pneumonia, circling, opisthotonus, recumbency, and death. Schizonts ofTheileria were detected in impression smears of the lymph nodes, spleen, and liver. Gross lesions include pale mucous membranes, enlarged lymph nodes, splenomegaly, and hepatomegaly with gall bladder distention. Pe...
Source: Comparative Clinical Pathology - May 10, 2019 Category: Pathology Source Type: research

Cutaneous horn ( cornu cutaneum ) in a Pakistani goat
In this report, this lesion was observed on the apex of the ear in a female Pakistani goat. The size of the cutaneous horn was 12  cm length. It was removed under local anesthesia and fixed in 10% neutral buffered formalin. Histopathologic examination showed well-differentiated keratinocytes without atypical keratinocytes. Thick dermal collagenous bundles were observed in the horn base. Mononuclear cell inflammation was obser ved around the vessels of the dermis in the base of lesion. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 10, 2019 Category: Pathology Source Type: research

Correction to: Evaluation of leukocyte esterase test strips for rapid diagnosis of subclinical mastitis in dairy cows
The affiliation of Abolfazl Hajibemani in the original published version of this article was incorrect. Instead of Tabriz University, it should have been University of Tabriz. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 10, 2019 Category: Pathology Source Type: research

Effects of medicinal plant powder as feed additives on growth performance, carcass characteristics, and immune response of Coturnix japonica against avian influenza and Newcastle disease vaccine virus
AbstractThe purpose of this study was to evaluate the potential effect of different levels ofGracilaria corticata (Gracilaria),Sargassum cristaefolium (Sargassum),Rhus coriaria (sumac), andPunica granatum (pomegranate) peel powdered on growth performance, carcass characteristics, and immune responses of Japanese quails against avian influenza and Newcastle disease killed vaccine virus. A total of 360 one-day-old Japanese quails were randomly distributed into nine dietary treatments with 4 replicates (10 quail/rep). Control group (C) received the diet free of medicinal plants supplementation and the other eight treatments r...
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

Changes in the cardiac phosphotransfer network associated with oxidative stress in experimentally infected Brucella ovis mice
AbstractThe aim of this study was to evaluate whetherBrucella ovis infection altered the activities of cardiac enzymes belonging to the phosphotransfer network and cause an oxidative imbalance in mice, used as an experimental model. We used hearts from 48 mice in two groups (n = 24 each): uninfected (control group) and infected byB. ovis, collected on days 7, 15, 30, and 60 post-infection (PI). Cardiac cytosolic creatine kinase (CK) activity was higher in infected animals than in the control group on days 30 and 60 PI, while cardiac mitochondrial CK activity was lower at the same moments of infection. Cardiac...
Source: Comparative Clinical Pathology - May 9, 2019 Category: Pathology Source Type: research

Prevalence of Dirofilaria immitis and Dirofilaria repens in outdoor dogs in Tehran Province, Iran
AbstractDirofilaria immitis andDirofilaria repens are the most common species of filarial nematodes described in dogs, which are endemic in Iran. The diagnosis of canine dirofilariosis is usually based upon the microscopical detection and identification of circulating microfilariae together with ELISA detection of serum circulating heartworm antigens or antibodies. The identification of the parasite species using the traditional approaches sometimes can lead to misdiagnosis. As a zoonotic disease, it is important to know the prevalence ofDirofilaria in Tehran that is the most crowded province with many outdoor and stray do...
Source: Comparative Clinical Pathology - May 8, 2019 Category: Pathology Source Type: research

Comparative study on hemato-biochemical alterations and selected acute phase protein response in native sheep experimentally infected with bluetongue virus serotypes 10 and 24
AbstractBluetongue (BT) is an infectious, non-contagious, insect-transmitted viral disease of sheep, other domestic, and wild ruminants caused by bluetongue virus (BTV), a prototype species of the genusOrbivirus of the familyReoviridae. The study was designed to determine the hematological and biochemical alterations and acute phase protein response in BTV sero-negative sheep experimentally infected with bluetongue virus serotype-10 and 24. The BTV-infected group comprising of 18 animals, 6 each inoculated with 6  ml of clarified virus containing 1 × 106/ml TCID50 of BTV-10 or 24 by intradermal rou...
Source: Comparative Clinical Pathology - May 8, 2019 Category: Pathology Source Type: research

The effect of Descurainia sophia oil on methamphetamine-induced cell cytotoxicity and cell death in PC12
In this study, protective effects ofDescurainia sophia oil were followed up in methamphetamine-induced cell cytotoxicity in a neuron-like PC12 cell line. The viability, proliferation, and cytotoxicity of the cells were assessed by Trypan blue, MTT test, and lactate dehydrogenase (LDH) assay, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was performed to detect DNA fragmentation and apoptosis. Nitric oxide (NO) level was detected by Griese method. Interleukin-1 β (IL-1β), IL-6, interferon gamma (INF-γ), and tumor necrosis factor alpha (TNF-α) pro-inflammatory c...
Source: Comparative Clinical Pathology - May 8, 2019 Category: Pathology Source Type: research

Aging of bovine spinal cord: an alteration of myelinated nerve fibers in the white matter
AbstractTo gain more knowledge of age-related changes in the spinal cord of the bovine species, the structure of 15 clinically normal aged cattle (Holstein-Friesian cows 8 –13 years of age) was histopathologically examined. In 12 (80%) of the 15 animals, the common histopathological features identified were alteration of a small number of myelinated nerve fibers in the white matter, with less than 4 nerve fibers affected per cross-sectional area of the spinal cord. Alteration of nerve fibers was characterized by dilation of myelin sheaths with loss of axons or macrophage infiltration, resembling features of Wall...
Source: Comparative Clinical Pathology - May 6, 2019 Category: Pathology Source Type: research

Anti-plasmodial, Anti-inflammatory, anti-nociceptive and safety profile of Maytenus senegalensis root bark extract on hepato-renal integrity in experimental animals
AbstractMaytenus senegalensis is a plant with several medicinal claims in folk medicine. Methanol root extract of the plant was evaluated for antiplasmodial, anti-inflammatory, anti-nociceptive, and safety profile. Antiplasmodial, anti-inflammatory (egg albumin induced paw edema), and analgesic (hot plate test) experiments were set up, each consisting of four (4) groups (A-D) of five mice each. Groups A –C of each experiment were treated with 2.0 ml/kg bw normal saline, 400 and 800 mg/kg bw of the extract according to the procedures. Chloroquine, acetyl salicylic, and aspirin respectively were used as the r...
Source: Comparative Clinical Pathology - May 2, 2019 Category: Pathology Source Type: research

Disseminated histoplasmosis in a Brazilian domestic cat: early diagnosis and successful treatment
AbstractHistoplasmosis is a worldwide distributed opportunistic mycosis caused by the dimorphic fungusHistoplasma capsulatum. It is reported in cats with non-specific clinical signs. The aim of this case report is to describe the first case of disseminated histoplasmosis in a cat in the city of Santa Maria, Rio Grande do Sul, Brazil. In veterinary consultation, it was reported that the animal had a history of vomiting, hematochezia, and weight loss for 3  months. At physical exam, an abdominal lymph adenomegaly, an intestine diffusely enlarged, and a poor body condition were observed. Ultrasound-guided fine-needle asp...
Source: Comparative Clinical Pathology - April 29, 2019 Category: Pathology Source Type: research

Ameliorative effects of alpha-lipoic acid and imidocarb dipropionate on clinico-haematological changes induced by experimental Babesia canis vogeli infection in dogs
In conclusion, co-administration of imidocarb with ALA acted synergistically and also ameliorated the clinico-haematological changes in experimental canine babesiosis, a nd the agent may be a promising and potent drug in the management of canine babesiosis. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 27, 2019 Category: Pathology Source Type: research

Assessing the possible causes of hemolytic anemia associated with lumpy skin disease naturally infected buffaloes
This study concluded that buffaloes show limited susceptibility to LSD, although the presence of hypophos phatemia could exaggerate hemolytic anemia under an oxidant-anti-oxidant imbalance and increased pro-inflammatory cytokines. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 27, 2019 Category: Pathology Source Type: research

Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles
The objective of this study was to evaluate and quantify the fat storage in the liver of lesser guitarfishZapteryx brevirostris submitted to different periods of restriction and refeeding. In a water recirculation system, 21 animals were divided into three groups: control (n=7), fed every day; W1 (n=7), with one week of restriction and two of refeeding and W2 (n=7), with two weeks of restriction and two of refeeding. After 76 days, the animals were euthanized and liver samples were collected, paraffin embedded, stained with PAS and evaluated with stereological procedures and histopatological changes were recorded. The area...
Source: Comparative Clinical Pathology - April 22, 2019 Category: Pathology Source Type: research

Systemic lipofuscinosis associated with a lesion of autophagic vacuolar myopathy in the diaphragmatic muscle of a cow
AbstractA 7-year-old Holstein-Friesian dairy cow that was culled because of traumatic motor dysfunction due to accidental falling down showed a variety of gross postmortem findings, such as fracture of the caudal vertebra, massive hemorrhage in the pelvic cavity, edematous swelling of the right sciatic nerve, and brown discoloration of the masseter muscle, tongue, cardiac muscle, diaphragmatic muscle, liver, adrenal cortex, and intestinal wall. Histopathologically, the discolored and some other internal organs/tissues exhibited varying degrees of intracellular deposition of lipofuscin granules. A small number of eosinophil...
Source: Comparative Clinical Pathology - April 16, 2019 Category: Pathology Source Type: research

Gastroprotective effects of mumie on aspirin-induced gastric lesions in rats
AbstractThe present research was carried out to investigate the influence of mumie aqueous extract on aspirin-induced gastric lesions in rats. Thirty-eight male animals were grouped as the following: Normal control received 1  ml normal saline; positive control received 1 ml normal saline; standard control received omeprazole (20 mg/kg); treatment received mumie (600 mg/kg). After 1 h all the rats (except the normal control group) were given 500 mg/kg aspirin to induce lesions. Four hours after lesion induction, th e gastric injuries were assessed through the evaluation of the macroscopic appe...
Source: Comparative Clinical Pathology - April 15, 2019 Category: Pathology Source Type: research

Diagnosis and treatment of canine leptospirosis due to serovar Bataviae —a case report
AbstractInfection ofLeptospira interrogans var. Bataviae in a properly vaccinated German shepherd dog and its successful clinical management are described. The dog was presented with a history of anorexia, vomiting, oliguria, melena, lethargy, and weakness. The animal had a proper history of yearly vaccination with the multicomponent vaccine. Hematology revealed leucocytosis, thrombocytopenia, granulocytosis, and anemia. Serum biochemical analysis showed elevated BUN, creatinine, ALP, total bilirubin, hypoalbuminemia, hyperglobulinemia, and lower A:G ratio. Based upon the clinical signs and blood-biochemical changes, the c...
Source: Comparative Clinical Pathology - April 13, 2019 Category: Pathology Source Type: research

The beneficial effect of equine chorionic gonadotropin hormone (eCG) on the in vitro co-culture of bovine spermatogonial stem cell with Sertoli cells
AbstractSpermatogenesis is a complex developmental process that originates from spermatogonia stem cell. This process consists of sequential, highly organized steps of cell proliferation and differentiation resulting in the generation of functional spermatozoa. Many growth factors, hormones, and cell interactions of germ cells with Sertoli cells regulate these processes, and the failure of any of the processes can lead to male infertility The aim of the present study was to determine the effects of equine chorionic gonadotropin on spermatogonial stem cell colony formation and differentiation after in vitro co-culture with ...
Source: Comparative Clinical Pathology - April 10, 2019 Category: Pathology Source Type: research

Oxidative stress and stroke: a review of upstream and downstream antioxidant therapeutic options
AbstractThe stroke is a cerebrovascular disorder and is the cause of a second mortality and long-lasting harm worldwide. It is classified into two types: hemorrhagic and ischemic stroke. Globally, 13% of cases of stroke are hemorrhagic, while the remaining 87% are ischemic. In this paper, we discuss the consideration of factors which possibly affect the effectiveness of antioxidant protection under oxygen deprivation, as well as oxidative stress. Such aspects as compartmentalization of reactive oxygen species (ROS)/reactive nitrogen species (RNS) formation and antioxidant localization, synthesis and transport of antioxidan...
Source: Comparative Clinical Pathology - April 9, 2019 Category: Pathology Source Type: research

Biochemical changes in Curimbat á subjected to transport stress and exposed to an agricultural fair
AbstractFish known as Curimbat á (Prochilodus lineatus) suffered a reduction in natural stocks due to predatory fishing and environmental deterioration; these are among the reasons for increased interest in the inclusion of this species in agricultural fairs. The aim of this study was to evaluate whether transport and exposure to an agricultural fair alters plasma biochemical parameters in curimbat ás. Blood samples were collected at 0 h (before transport), 10 h (end of transport), and 72 h (end of exposure to agricultural fair). Plasma cortisol levels increased after 72-h exposure to agricultu...
Source: Comparative Clinical Pathology - April 5, 2019 Category: Pathology Source Type: research

Investigations on the antimicrobial and wound healing activity of Ficus thonningii Blume extracts
In conclusion, 7.5% ointment prepar ation of ethyl acetateF. thonningii leaf extract could be considered as a potent herbal drug for treating wound infections. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 2, 2019 Category: Pathology Source Type: research

Metabolic status and ultrasound traits of reproductive tract of estrus dairy cows in early postpartum period
This study indicated that the correlation between ultrasound traits of uterus and metabolite hormones was not significant at the clean test in estrus dairy cows. Moreover, there was no relationship between serum leve l of IGF-1, NEFA, and BHBA with either dominant follicle diameter or existence of corpus luteum (CL). Significant association between ultrasound properties of the uterus and ovaries and metabolic hormone (IGF-1, NEFA, and BHBA) concentrations did not reveal in estrus dairy cows at day 28 ± 3 po stpartum. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - April 2, 2019 Category: Pathology Source Type: research