Serum concentration of 25 (OH) vitamin D in healthy dogs: factors as age, sex, and diet
This study aimed at determining serum 25(OH)D values in healthy dogs and evaluating its association with factors such as sex, age, and diet. Blood samples were collected from healthy intact male and female dogs divided into two groups: (G1) 30 dogs (Beagles), 15 adults and 15 puppies, receiving standardized diet; (G2) 29 dogs of different breeds, adults, receiving varied diets. The 25(OH)D was analyzed by the chemiluminescence method. Serum levels of 25(OH)D are not associated with sex, but the concentration is lower in puppies (30.6 ng/mL) than in adults (34.9 ng/mL) (G1),P
Source: Comparative Clinical Pathology - May 18, 2020 Category: Pathology Source Type: research

Purple onion in combination with garlic exerts better ameliorative effects on selected biomarkers in high-sucrose diet-fed fruit fly ( Drosophila melanogaster )
AbstractGarlic and purple onion are two vegetables widely used globally for culinary purposes. These vegetables have been previously reported to exhibit several medicinal properties including antidiabetic, antihypertensive, and renoprotective properties owing to their constituent bioactive phytochemicals. However, both vegetables are often combined in popular culinary practices and there is a dearth of information on their combinatorial effects. Therefore, this study evaluated the effect of garlic, onion, and their combination on high-sucrose diet (HSD)-Drosophila melanogaster model. HSD-drosophila is gaining popularity as...
Source: Comparative Clinical Pathology - May 18, 2020 Category: Pathology Source Type: research

Osteosarcoma in a Dachshund: cytological and histopathological aspects
This report alerts clinicians to the occurrence of osteosarcoma in small animals and demonstrates the importance of fine-needle aspiration cytology as a tool in the diagnosis of bone neoplasms. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - May 17, 2020 Category: Pathology Source Type: research

Clinicopathological features, molecular diagnosis and treatment of epizootic lymphangitis in a donkey at a university zoological garden: a case report
AbstractReport on epizootic lymphangitis, a chronic contagious zoonosis ofEquidae caused byHistoplasma capsulatum var.farciminosum characterized clinically by pyogranulomatous multifocal dermatitis with lymphadenitis, is rare in donkeys. This case reports a 6-year-old, 200-kg local male donkey at the University of Ibadan zoological garden with wounds on its dorsum which developed into ulcerative lesions on the left cranial thoracic region. The lesions were also along the lymphatic drainage of the left infrascapular area down to the girth with extensive nodular lesions on the skin, characteristic focal ulcers and fenestrati...
Source: Comparative Clinical Pathology - May 16, 2020 Category: Pathology Source Type: research

Pathological findings in a fatal pet rabbit Pasteurellosis
AbstractA case of fatal Pasteurellosis infection in a 2-month-old rabbit is described. Pasteurellosis is considered one of the most important diseases of rabbits and has been reported as a constant serious and highly contagious disease of domestic rabbits. Before death, the main clinical signs observed were rhinitis with nasal discharge, abdominal breathing and coughing. At necropsy, pathological findings revealed severe pleuropneumonia with the accumulation of a remarkable amount of fibrinopurulent exudate in the thoracic cavity, serous rhinitis and tracheitis. A bacterial isolate from lungs was identified asPasteurella m...
Source: Comparative Clinical Pathology - May 16, 2020 Category: Pathology Source Type: research

Note on the Mesopotamian spiny-tailed lizard, Saara loricata (Blanford, 1874): morphometrics and evidence for gender partitioning of hematological data
The objectives of this study were to document morphometrical measurements and hematological data of clinically healthy free-ranging adult Mesopotamian spiny-tailed lizards (Saara loricata), the Iraqi Mastigure or Iraqi spiny-tailed lizard, from Khuzestan Province, Iran, and to investigate sex differences in evaluated parameters. Although we did not observe any gender differences in morphometrical measurements, the visually larger genital pores of males allowed for differentiation of study animals into females (n = 6) and males (n = 5). We obtained femoral vein blood samples for hematological ana...
Source: Comparative Clinical Pathology - May 7, 2020 Category: Pathology Source Type: research

Assessment of biochemical and immunohistochemical changes in buffalo-cows with reproductive disorders
In this study, 25 buffalo-cows (normal cyclic,n = 9, pluriparous; anestrus,n = 7, nulliparous and repeat breeders,n = 9, pluriparous) were used to assess some biochemical and immunohistochemical changes in those that had reproductive disorders in comparison with normal cyclic animals. Serum calcium (Ca) and vitamin D (25(OH)D) levels were analyzed. Also, serum progesterone (P4) and estradiol (E2) levels were measured to assess the animals’ reproductive statuses. Additionally, immunohistochemical expression of calcium-binding protein-D9k (CaPB-9k) and vitamin D receptor (VDR) ...
Source: Comparative Clinical Pathology - April 30, 2020 Category: Pathology Source Type: research

Complete blood count evaluation of dogs treated with four different antineoplastic chemotherapy protocols
AbstractThis retrospective study aimed to evaluate potential hematological alterations in dogs treated with four different antineoplastic protocols. We selected 69 dogs that received at least four chemotherapy sessions and divided them into four groups: vinblastine (VBL) (n = 30), doxorubicin (DOX) (n = 15), combined CHOP protocol (cyclophosphamide, doxorubicin, vincristine, and prednisone) (n = 11), and vincristine group (VCR) (n = 13). Hematological parameters were recorded from blood samples collected in five periods: T0 (before the start of treatment), T1, T2, T3, and T4 ...
Source: Comparative Clinical Pathology - April 24, 2020 Category: Pathology Source Type: research

Effects of pirfenidone, vitamin E, and pirfenidone –vitamin E combination in paraquat-induced pulmonary fibrosis
AbstractParaquat-induced pulmonary fibrosis is a progressive and fatal interstitial lung disease, a condition for that, there is no effective treatment and its prognosis is appalling. Since multiple coactivated pathways are involved, targeting these pathways using combination regimens is plausible for successful therapy. So, the aim of the present study was to evaluate the therapeutic efficacy of pirfenidone –vitamin E combination therapy in (paraquat) PQ-induced lung fibrosis model. After the development of PQ-induced lung fibrosis, pirfenidone, vitamin E, pirfenidone plus vitamin E, and water (as a vehicle) were or...
Source: Comparative Clinical Pathology - April 24, 2020 Category: Pathology Source Type: research

Comparative effect of quail and chicken egg consumption on lipid profile, total proteins and blood parameters of male albino rats ( Rattus norvegicus )
This study evaluated the comparative effect of feeding quail egg or chicken egg on the lipid profile, total proteins and blood parameters of male albino rats. Male albino rats (36) weighing between 100 and 120  g were used for this study. They were randomly assigned into 7 groups of 5 rats each. Groups A1, A2 and A3 were dosed with quail egg at 0.25 mg/kg, 0.5 mg/kg and 0.75 mg/kg, respectively. Groups B1, B2 and B3 received 0.25 mg/kg, 0.5 mg/kg and 0.75 mg/kg, respectively, of chicken egg. The co ntrol group received distilled water. Blood for haematology, lipid profile and total protei...
Source: Comparative Clinical Pathology - April 19, 2020 Category: Pathology Source Type: research

Parkinsonia aculeata L. aqueous extract alleviated the hepatotoxicity induced by acetaminophen in albino rats
AbstractHepatotoxicity is considered a serious condition which can be progressed to liver damage. The hepatoprotective effect ofParkinsonia aculeata (PA) aqueous extract against acetaminophen (APAP)-induced hepatotoxicity was investigated. Wister albino rats were orally pre-treated with PA aqueous extract (250  mg/kg BW) for 14 days. A single toxic dose of APAP (800 mg/kg BW) was orally administered at the 13th day. Collection of blood samples was done for the biochemical analysis, and the hepatic tissue was collected for histopathological examinations. Our results indicate that PA aqueous extract exhi bit a...
Source: Comparative Clinical Pathology - April 16, 2020 Category: Pathology Source Type: research

Pathological and molecular detection of a rare case of cutaneous cryptococcosis in a 9-year-old horse
This report describes a rare case of primary cutaneous cryptococcosis in equines. A young gelding horse was presented with facial masses showing ulcerations and yellowish discharge. Histopathological examination of biopsy specimens showed a dermal granulomatous reaction containing neutrophils, macrophages, and giant cells. In addition, numerous spherical yeast-like organisms were clearly observed within the granulomatous reaction. We suspected that the observed organisms belong to theCryptococcus spp., which was indeed by its shape on Sabouraud dextrose agar, Gram-stained smear suggestiveC. neoformans, and confirmed on PCR...
Source: Comparative Clinical Pathology - April 14, 2020 Category: Pathology Source Type: research

The effects of selenium nanoparticles (SeNPs) on oxidant and antioxidant activities and neonatal lamb weight gain pattern
AbstractSelenium (Se) is an antioxidant element that prevents the oxidative stress. Se incorporates into proteins such as selenocysteine and prevents oxidative damage to body tissues. In neonatal period, the different types of stress such as hypoglycemia and hypothermia may predispose them to many types of infectious diseases and also reduce growth rate at postnatal period. For these reasons, the present study was conducted on 12 newborn lambs to evaluate the effects of oral selenium nanoparticle (SeNP) supplementation on serum levels of selenium, copper, zinc, thiobarbituric acid reactive substances (TBARS), superoxide di...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Glucose levels are stable in stored blood and plasma samples of cultured female African catfish ( Clarias gariepinus )
AbstractGlucose is utilized by fish as one of the sources of energy. It is a physiological parameter useful in the determination of the general health condition of cultured fish. The aim of this study was to determine the stability of plasma and blood glucose concentration, stored at different temperatures (4 °C and 30 °C) for 72 h. Two (2) milliliters of blood was collected via the caudal abdominal venipuncture of 10 female catfishes purchased. The fish were 8 months of age and were kept in plastic ponds at the Faculty of Veterinary Medicine, University of Nigeria. Blood was collected in blood tubes c ontaining K2...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Short-term effects of feeding powdered Aloe vera ( Aloe barbadensis ) and nettle ( Urtica dioica ) on growth performance and stimulation of innate immune responses in rainbow trout ( Oncorhynchus mykiss )
AbstractTo evaluate the nutritional impacts of powdered Aloe vera and nettle plants on growth performance and some parameters of immune response in rainbow trout, a total of 462 fish with an average weight of 10.80  ± 0.59 g were obtained from authentic fish farms. The fish were fed in seven groups with diets containing 0.5, 1, and 1.5% of Aloe vera powder and 0.5, 1, and 1.5% of nettle powder, and also a control with a basal diet without herbal supplementation for 4 weeks. The results of fish biometry showed that dietary supplementation with the powdered plants led to significant differences...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Effect of high-intensity interval training on expression of microRNA-149 and genes regulating mitochondrial biogenesis in doxorubicin-cardiotoxicity in rats
AbstractWe have investigated the effect of high-intensity interval exercise (HIIT) on DOX-induced cardiotoxicity and the expression of mitochondrial biogenesis genes SIRT-1, PGC-1 α, and NRF-2 as well as microRNA-149-5p in a rat model. Twenty-four male Wistar rats (250–270 g) were randomly divided in four groups (n = 6/each): control, HIIT, DOX, and HIIT+DOX. HIIT was performed on a treadmill as seven alternative intervals of high and low intensity trainings for 1 h a day for 6 weeks. After the last session of HIIT, DOX was injected i.p. to trained and time-matched control rats. Aft...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Biochemical and total hemocyte count profiles of long-clawed crayfish ( Astacus leptodactylus ) as a reference interval in southern Iran
This study was aimed to determine the normal interval of the biochemical parameters in these species in Iran, which appears to be necessary for future plannin g and studies, especially for ecotoxicological studies. Biochemical parameters analyzed in this study were glucose (11.37 ± 1.53 mg/dl), urea (5.58 ± 0.62 mg/dl), creatinine (0.21 ± 0.02 mg/dl), AST (7.54 ± 0.82 U/L), ALT (7.04 ± 0.97 U/L), ALP (380.44 ± 33.46 U/L), total bilirubin (0.45 ± 0. 32 mg/dl), calcium (8.39 ± 1.15 mg/dl), phosphorus (2.35 ± 0.24 mg/dl), total protein (1.72 ± 0.16 g/dl), a...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Murine leukocytes stimulation by Toxoplasma gondii antigens induces high level of IFN- γ production
AbstractToxoplasma gondii (T.gondii) is an obligate intracellular parasitic protozoan, infecting a wide range of warm-blooded vertebrates. It is important forT. gondii to balance the response between cellular and humoral immune system for various aspects of its own survival. The aim of this study was to determine the level of IFN- γ and IL-4 production by murine peritoneal leukocytes stimulated withT. gondii lysate antigens (TLAs), excretory/secretory antigens (ESAs), and a combination of them (TLAs:ESAs). TLAs, ESAs, and TLA  + ESA, considered as the experimental groups, were prepared and used to stimu...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Neurobehavioural and repeated-dose toxicity studies on the extractive from the decoction of a mixture of Alstonia boonei and Picralima nitida in mice
This study evaluated the central nervous system effects and the toxicity of the remedy on acute and repeated doses with a view to providing information on its safety. The powder mixture (ratio 1:2) ofA. boonei stem-bark andP. nitida seed was extracted with water by the decoction method, concentratedin vacuo and freeze-dried. The acute toxicity of the extractive was determined by Lorke ’s method. For the novelty-induced behaviour tests, 24 mice (18–22 g) of four groups (six mice per group) were orally given the aqueous solution of the extractive at 12.5, 25 and 50 mg/kg while the control group was given distille...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Circulatory levels of pro-inflammatory cytokines (IL-6 and IL-1 β) and neutrophil-lymphocyte ratio (NLR) in diabetic patients in Nigerian population
This study was designed to evaluate pro-inflammatory cytokine levels (IL-6 and IL-1 β) and NLR in T2DM subjects in Nigerian population. This was a case-control study involving 75 physician diagnosed T2DM subjects attending the Endocrine Clinic of a Tertiary Health Institution in Nigeria and 75 age- and sex-matched healthy individuals (used as control) who were recruited from the B lood Donor Center of the same institution. Based on physician assessment, the diabetic subjects were grouped differently into those with complications (48) and those without complications (27). Serum levels of IL-6 and IL-1β were measur...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Anti-inflammatory activities of crude ethanol extract of Combretum zenkeri Engl. & amp; Diels leaves
ConclusionThis study shows that EECZ possess anti-inflammatory activities that could be through the inhibition of PLA2 activity and platelet aggregation, and membrane stabilization. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

Effect of cannabinoid-serotonin interactions in the regulation of neuropeptide Y1 receptors expression in rats: the role of CB1 and 5-HT 2C receptor
AbstractNeuropeptide Y (NPY) is involved in a diversity of critical functions such as circadian rhythms, energy homeostasis, and appetite regulation in the hypothalamus. It has identified as a crucial participant in adjusting energy intake and energy storage as fat via central neuropeptide Y1 receptor (NPY1R), leading to obesity and metabolic disorders. The present study was expected to investigate the interaction between 2-AG (CB1R agonist), m-CPP (5HT2CR agonist), SB-242084 (5HT2CR antagonist), and SR-141716A (CB1R antagonist) by mediating through the NPY1R for treating or preventing obesity, metabolic disorders, and oth...
Source: Comparative Clinical Pathology - March 16, 2020 Category: Pathology Source Type: research

First report of pedunculate barnacle ( Octolasmis neptuni ), as potential asymptomatic carrier of white spot syndrome virus (WSSV)
AbstractWhite spot syndrome virus (WSSV) is a notorious virus infecting cultured shrimps affecting the aquaculture industry for more than two decades over the world. WSSV has been isolated from asymptomatic carriers including crabs, artemia, rotifers, polychaetes and insects. Asymptomatic carriers are hosts that do not exhibit infection symptoms but carries infection to other hosts. The potential virulence of asymptomatic carrier of WSSV in multiple hosts were screened in the current study. The symbioticOctolasmis neptuni isolated from the gill lamellae of moribund mud crab (Scylla olivacea) is suspected to cause WSSV infe...
Source: Comparative Clinical Pathology - March 6, 2020 Category: Pathology Source Type: research

Neuroprotective effects of gallic acid against neurotoxicity induced by sodium arsenite in rats
AbstractArsenic causes a wide range of neurological complications including cognitive impairment, short-term memory and concentration problems. The present study investigated effects of gallic acid (GA) against sodium arsenite (SA)-induced neurotoxicity in rats. Thirty-five adult male rats were randomly divided into five groups. Group 1 received normal saline (2  ml/kg, P.O.) for 21 days. Group 2 received SA (10 mg/kg, P.O., for 14 days). Groups 3 and 4 received GA (10 and 30 mg/kg, P.O., respectively) for 7 consecutive days prior to SA treatment and continued up to 21 days, parallel to SA adm...
Source: Comparative Clinical Pathology - March 2, 2020 Category: Pathology Source Type: research

Methanol extract of Napoleona vogelii demonstrates anti-nociceptive and anti-inflammatory activities through dopaminergic mechanism and inhibition of inflammatory mediators in rodents
This study was conducted to investigate the anti-nociceptive and anti-inflammatory activity of the methanol stem bark extract ofN. vogelii in rodents. The extract (100 –400 mg/kg) was administered 1 h before intraperitoneal and intraplantar injection of 0.2 mL of 0.6% v/v acetic acid and 0.05 mL of 2% v/v formalin, respectively. Anti-inflammatory activity was investigated using the carrageenan-induced paw edema and formalin-induced arthritis models. Mechanis tic studies were conducted by exploring opioidergic, dopaminergic pathways, and inflammatory mediators. There was 62.26% inhibition of inflam...
Source: Comparative Clinical Pathology - February 28, 2020 Category: Pathology Source Type: research

Correction to: Anticipation of estrus by the use of bromocriptine (Parlodel 2.5 mg) in Atlas Shepherd bitch: cytological and echographic follow-up
The author of this article would like to add another affiliation, which is reflected here. This correction does not, in any way modify or change the scientific validity of the results presented in this manuscript. The author sincerely apologizes for this error. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 27, 2020 Category: Pathology Source Type: research

Vitamin D dietary supplementation ameliorates the complications associated with type 2 diabetes induced by streptozotocin
AbstractType 2 diabetes is a major public health problem, accounting for significant premature mortality and morbidity. It has been reported to be associated with several chronic complications which involve coronary artery disease and renal and liver diseases that are considered the primary cause of disability and mortality in diabetic patients. Different studies stated that vitamin D have functional role in glucose tolerance through its effects on insulin secretion and sensitivity and ameliorate type 2 diabetes complication. For that this study was carried out to investigate the effect of dietary vitamin D on type 2 diabe...
Source: Comparative Clinical Pathology - February 13, 2020 Category: Pathology Source Type: research

Jeopardy of Lyssavirus infection in relation to hemato-biochemical parameters and diagnostic markers of cerebrospinal fluid in rabid calves
AbstractRabies is caused by a single-stranded virus with ribonucleic acid belonging to the genusLyssavirus in theRhabdoviridae family. This disease causes 100% fatality of exposed non-vaccinated humans and animals. Many aspects of rabies still ambiguous; therefore,  the hemato-biochemical effects ofLyssavirus infection and  the potential diagnostic markers of cerebrospinal fluid (CSF) in rabid calves were investigated. Six female Frisian cross-bred calves (6–7 months of age) were accidentally exposed to bites from stray dogs. Twenty days later, the calves showed anorexia, severe ptyalism...
Source: Comparative Clinical Pathology - February 6, 2020 Category: Pathology Source Type: research

Haematological protective effect of raw extract and nano- Eclipta alba –treated rats in experimentally induced hepatocarcinogenesis
The objective of the present study was to evaluate the effect ofEclipta alba (L.) andE. alba nanoparticles on haematological and hepatoprotective parameters in diethyl nitrosamine (DEN) –induced toxicity and hepatocarcinogenesis model in rats. Eighty-one Wistar albino rats were divided into four groups. Group I control (n = 19), group II DEN .01% in water (n = 19), group III (DEN+ rawE. alba 50  mg/kg BW/day) and group IV (DEN+ nano-E. alba 50  mg/kg BW/day). DEN group (II) showed a significant (P 
Source: Comparative Clinical Pathology - February 6, 2020 Category: Pathology Source Type: research

Fasting hyperglycaemia, glucose intolerance and pancreatic islet necrosis in albino rats associated with subchronic oral aluminium chloride exposure
AbstractExposure to aluminium is associated with altered glycaemia, but this effect in relation to glucose tolerance and pancreatic islet pathology requires validation. The study was aimed at assessing the glycaemic changes, glucose tolerance and islet morphology of albino rats during oral aluminium chloride exposure. Twenty male albino rats weighing 189 –270 g were randomly divided into two equal groups. The control group was sham-treated with normal saline and the treated group was administered aluminium chloride solution (100 g/L) by gavage at 12.5% of LD50 (50  mg/kg) daily for 28 days. Fastin...
Source: Comparative Clinical Pathology - January 9, 2020 Category: Pathology Source Type: research

Clinical blood gas indices and histopathological effects of intrathecal injection of tolfenamic acid and lidocaine Hcl in donkeys
AbstractThe present study aimed to investigate the clinical blood gas indices and histopathological consequences after intrathecal injection of tolfenamic acid and lidocaine Hcl and moreover, to elucidate the spinal safety of tolfenamic acid as a cyclooxygenase inhibitor in donkeys. Ten clinically healthy donkeys were divided into two groups, 5 animals each. The first group received lidocaine Hcl 2% and the second one received tolfenamic acid 4% intrathecally. The physical parameters and ataxia, analgesia, and motor blockade scores were recorded. Blood gases and acid base balance indices and histopathological examination w...
Source: Comparative Clinical Pathology - January 9, 2020 Category: Pathology Source Type: research

Haematology, plasma biochemistry and serum of table size African catfish, Clarias gariepinus , naturally infected with Listeria species in Oyo State
This study evaluated the haematology, plasma biochemistry and blood enzymes of farm-raised African catfish,Clarias gariepinus, naturally infected with listeriosis in Oyo State. Fish were collected from 36 farms using stratified method. A total of 108 fish were collected where 37 were positive for listeriosis. The blood of the affected fish was used for this study. Haematology, plasma biochemistry and blood enzyme analysis were determined. Fish from Oyo zone had the highest PCV, Hb, RBC and MCHC with 29.33  ± 2.96%, 10.04 ± 1.04 dL, 3.55 ± 0.09 &time...
Source: Comparative Clinical Pathology - January 9, 2020 Category: Pathology Source Type: research

Bovine dilated cardiomyopathy with overlapping myocarditis in an adult Holstein-Friesian dairy cow
AbstractIn humans, it is acknowledged that dilated cardiomyopathy is also caused by infection-induced myocarditis. To evaluate whether the occurrence of lesions of dilated cardiomyopathy in cattle may be triggered by myocarditis, an adult Holstein-Friesian dairy cow which showed dilated cardiomyopathy associated with myocarditis was examined by histopathology. Traumatic pericarditis or idiopathic congestive heart failure was clinically suspected, and the animal was culled because of unfavorable prognosis. Histopathological examination revealed an intrinsic cardiomyopathic lesion that overlapped with myocarditis. Cardiomyoc...
Source: Comparative Clinical Pathology - January 9, 2020 Category: Pathology Source Type: research

Oxidative stress in liver of grass carp Ctenopharyngodon idella naturally infected with Saprolegnia parasitica and its influence on disease pathogenesis
AbstractFungal diseases are one of the major problems in freshwater fish culture and have been linked to significant losses and high mortality rate, and saprolegniosis caused bySaprolegnia parasitica is the most important oomycete affecting freshwater fish worldwide. The hepatic pathophysiology of infection remains poorly understood and is limited only to histopathological alterations. Thus, the aim of this study was to evaluate whether tissue oxidative damage can be considered a pathway linked to liver damage duringS.parasitica infection in grass carp (Ctenopharyngodon idella). Grass carp was divided into two groups (n&th...
Source: Comparative Clinical Pathology - January 6, 2020 Category: Pathology Source Type: research

Alterations in hematological and biochemical parameters and DNA status in mice bearing Ehrlich ascites carcinoma cells and treated with cisplatin and cyclophosphamide
AbstractChemotherapy medicates cancer via killing cells. It may destroy both cancer and normal cells. This research was performed to investigate the  influences of cisplatin and cyclophosphamide on Ehrlich ascites carcinoma (EAC) bearing mice through some hematological, biochemical, molecular, and pathological studies. A total of 80 healthy female Swiss mice were equally divided into four groups. Group 1 was kept as the negative control. Grou ps 2 to 4 were injected intraperitoneally (IP) with 2.5 × 106 EAC where mice in group 2 were kept as the positive control without treatment. Groups ...
Source: Comparative Clinical Pathology - January 3, 2020 Category: Pathology Source Type: research

Pathogenicity, haematology and pathology of Listeria monocytogenes in rat
AbstractListeria monocytogenes is a gram positive bacterium of public health concern with low morbidity but high mortality rate. Listeriosis in human is usually caused by the consumption of infected animal and food. It is noteworthy that an infected animal such as rat could be potential carrier and vector. Therefore, this study examined the clinic pathological changes associated withListeria monocytogenes infection in rat. Six (6) Winstar laboratory rats divided into 2 groups. Group 1 was challenged with 0.1-mL saline water as control while the second group was challenged intraperitoneally with 0.1  mL molecularly cha...
Source: Comparative Clinical Pathology - January 2, 2020 Category: Pathology Source Type: research

Determination of histopathological and biomedical parameters in protective effects of Petroselinum crispum on hepatotoxicity induced by dichlorvos in male Wistar rats
This study examines the detoxification feature of PC on Dich-induced hepatotoxicity in male Wistar rats. Sixty-four male Wistar rats were grouped into eight including sham (normal saline), Dich (4  mg/kg/day), PC 1, 2, 3 (100, 150, 200 mg/kg/day, respectively) and Dich+PC 1, 2, 3 (100, 150, 200 mg/kg/day, respectively). All treatments were applied intraperitoneally (i.p) at 10 A.M. daily for 4 weeks. A day after the last treatment, the animals were sacrificed by cervical dislocation. The histopathological changes and apoptosis processes were assessed by light and fluorescent microscopy methods. The...
Source: Comparative Clinical Pathology - January 2, 2020 Category: Pathology Source Type: research

Health performance and blood profile changes in commercial broilers supplemented with dietary monocalcium phosphate
AbstractFast-growing broilers faced with various leg health problems are in crucial need of an adequate nutritional supply of both calcium and phosphorus. The aim of the current work was to study the effect of different level of monocalcium phosphate supplementation on the health performance, haematology, and biochemistry changes of commercial broiler chickens. A total of 108 broiler birds (Cobb 500) were randomly divided into three treatment groups with six replicates consisting of six birds per replicates. Treatment 1 (control) was fed with available commercial starter and finisher diets containing dicalcium phosphate. T...
Source: Comparative Clinical Pathology - December 24, 2019 Category: Pathology Source Type: research

Impact of dietary bee honey during first weeks of life in dairy lambs on growth and metabolism
The objective of this study was to determine whether a daily dose of honey would improve productive performance as well as energy, protein, and lipid metabolisms in lactating lambs. Bee honey (fromApis mellifera) was offered orally for the first 7  days of the animals’ lives. Twenty Lacaune lambs were used, allocated to two groups: group C, lambs not supplemented with honey, and group T, consisting of lambs fed daily doses of 10 mL of honey. The experimental period lasted 45 days. Body weight measurements and blood sample collections wer e performed at five time points (days 1, 7, 15, 30, and 45 of lif...
Source: Comparative Clinical Pathology - December 21, 2019 Category: Pathology Source Type: research

Rare case presentation: DLBCL and plasma cell myeloma coexisting together
AbstractThe synchronous presence of dual hematological malignancy is an uncommon finding. In the present case, an 82-year-old male, presented with lymph nodal mass which was diagnosed as diffuse large B cell lymphoma (DLBCL) on histopathology and plasma cell myeloma (PCM), was diagnosed with identification of clonal plasma cells in the bone marrow. Interestingly, both clonal plasma cells and lymphoma cells showed lambda light chain restriction. The patient also developed spontaneous tumor lysis syndrome which can be seen in aggressive hematopoietic malignancies. The present case is a rare presentation of synchronous dual h...
Source: Comparative Clinical Pathology - December 21, 2019 Category: Pathology Source Type: research

Experimental hypothyroidism in adult male rats: the effects of Artemisia dracunculus aqueous extract on serum thyroid hormones, lipid profile, leptin, adiponectin, and antioxidant factors
This study lasted for 90 days. The animals were divided into six experimental groups (eight rats per each group): (1) normal control; (2) hypothyroid rats + levothyroxine, as positive control (4 μg/100 g/day); (3) hypothyroid rats, as negative control; (4) hypothyroid rats + tarragon aqu eous extract (100 mg/kg); (5) hypothyroid rats + tarragon aqueous extract (200 mg/kg); and (6) hypothyroid rats + tarragon aqueous extract (300 mg/kg). Blood samples were obtained using cardiac puncture. In blood samples, thyroid hormones (triiodothyronine (T3), free triiodothyronine (fT3), thyro xine (T4),...
Source: Comparative Clinical Pathology - December 14, 2019 Category: Pathology Source Type: research

Apoptosis and regeneration of hepatocytes in rats orally exposed to caffeinated energy drinks
AbstractCaffeinated energy drinks (CEDs) have become extremely popular over the last decade. In recent years, many researchers have raised a concern about the safety of CEDs. The aim of our study was to evaluate the morphology of the liver, as well as the features of hepatocyte apoptosis/regeneration, in rats orally exposed to CEDs for 2  weeks. Sixteen WAG rats formed two equal groups. Rats from the experimental group were orally exposed to a CED at a dose of 12 ml/kg of body weight for 2 weeks. The control group consisted of intact animals with the provided access to drinking water instead. The content of ...
Source: Comparative Clinical Pathology - December 14, 2019 Category: Pathology Source Type: research

The effect of methadone, buprenorphine, and shift of methadone to buprenorphine on sperm parameters and antioxidant activity in a male rat model
In conclusion, methadone and buprenorphine enhanced testicular oxidative stress, suppressed blood levels of sexual hormone, including LH, FSH, TT, and FT, and resulted in suppressed sperm parameters. There wer e no significant differences between methadone and buprenorphine treatments in terms of their adverse effects on sperm parameters and antioxidant activity. However, shift of methadone to buprenorphine resulted in the worst adverse effect in the male reproductive system in the rat model. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - December 12, 2019 Category: Pathology Source Type: research

FSH and estradiol benzoate administration recover spermatogenesis and sexual hormone levels in a busulfan-injured rat model
AbstractBusulfan induces azoospermia and testicular atrophy, while follicle stimulating hormone (FSH) and estradiol benzoate (EB) may improve spermatogenesis. Our objective was to determine the effects of FSH and EB on the recovery of spermatogenesis, histology, sexual hormones, and testicular gene expression in the testis of a busulfan-injured rat model. Forty adult rats were assigned into eight groups. Half received a single intraperitoneal saline or 30  mg/kg busulfan injection, and 42 days after, the animals received intramuscular injections for 10 days: 1 ml saline, 7.5 IU FSH, and 12 &mu...
Source: Comparative Clinical Pathology - December 3, 2019 Category: Pathology Source Type: research

Report and abstracts for 20th ACCP conference with training workshops, and technology exhibits
AbstractA report is made on the 2-day, 20th annual conference held by the Association for Comparative Clinical Pathology (ACCP). This included two workshops that were given by veterans of the toxicologic clinical pathology community including on haematotoxicity and on the interpretation of clinical pathology data of pre-clinical toxicology studies in support of pharmaceuticals in development. Also, included was a series of scientific and technology presentations on the latest developments in toxicologic and veterinary clinical pathology. Key developments presented on were the invention of a rapid and simple diagnostic test...
Source: Comparative Clinical Pathology - December 3, 2019 Category: Pathology Source Type: research

Clinical pathology and health reference values for loggerhead sea turtles ( Caretta caretta ) and olive ridley turtles ( Lepidochelys olivacea ) in the Gulf of Ulloa, Baja California Sur, Mexico
AbstractAnthropogenic impact and environmental threats can cause diseases to marine turtles and in severe cases death, contributing to population decline worldwide. The Gulf of Ulloa (GU) represents an important foraging habitat for loggerhead sea turtles and olive ridley turtles; it is also considered a highly productive area for fisheries and is known for the mortality of marine turtles, which has been associated to bycatch. However, there is little information in the area regarding the health status of marine turtles; thus, the aims of this study were to (1) assess the health of marine turtles via physical examination, ...
Source: Comparative Clinical Pathology - October 29, 2019 Category: Pathology Source Type: research

Antifungal activity of ethanolic extracts of Ocimum gratissimum and Vernonia amygdalina leaves against dermatomycotic agents isolated from domestic animals in South Eastern Nigeria
This study aims to isolate dermatomycotic agents from domestic animals and evaluate, using ethanolic leaf extracts ofOcimum gratissimum andVernonia amygdalina, the in vitro antifungal profile of these extracts against these isolates. A total of 698 domestic animals (dogs, sheep, goats, and pigs) were examined for skin lesions suggestive of dermatomycosis and skin scrapings collected. The agar well diffusion assay was used to evaluate the in vitro activity of both leaf extracts using voriconazole and their solvent, dimethyl sulfoxide (DMSO), as control positive and negative respectively. Results showed that among the fungal...
Source: Comparative Clinical Pathology - October 29, 2019 Category: Pathology Source Type: research

Haematological reference values of dromedary camels in northern Nigeria
AbstractThe aim of this study was to determine the haematological reference values of a population of dromedary camels (Camelus dromedarius) in northern Nigeria to provide data required during laboratory investigation of health. The haematological variables were determined with EDTA anti-coagulated blood samples collected from the jugular veins of 121 camels during antemortem sessions at the abattoir in Maiduguri (Borno State). Serum total protein, albumin and globulin concentrations were estimated with serum samples harvested from clotted blood samples collected from the same animals. The haematological data were normally...
Source: Comparative Clinical Pathology - October 29, 2019 Category: Pathology Source Type: research

Evaluation of anti-inflammatory and analgesic activities of selected antibiotics in Wistar Albino rats
AbstractAnti-inflammatory, analgesic, and leukocyte mobilization activities of ciprofloxacin, lincomycin, and erythromycin were evaluated in this study. The study involved two sets of thirty-three adult female Wistar rats assigned into five treatments, grouped into eleven of three rats per group. For agar-induced paw edema, the first three groups were treated 10  mg/kg, 20 mg/kg, and 40 mg/kg of ciprofloxacin, next three groups same doses of lincomycin, next three groups same doses of erythromycin, the tenth group received the reference drug, indomethacin 5 mg/kg while the last group 5 mg/kg normal...
Source: Comparative Clinical Pathology - October 29, 2019 Category: Pathology Source Type: research

A survey of leukocyte profiles of red-backed salamanders from Mountain Lake, Virginia, and associations with host parasite types
AbstractIn this project, red-backed salamanders,Plethodon cinereus, were collected from the heart of their range (Mountain Lake, Virginia, USA), in an effort to document the typical, or baseline, leukocyte profile of this species in its natural state. From microscopic examination of thin blood films, we determined relative proportions of all leukocyte types and determined the ratio of neutrophils to lymphocytes, which is a useful index of stress levels. In addition, individuals were examined for three naturally occurring endoparasites: two gastro-intestinal (trematodes and a ciliated protozoan in the genusCepedietta) and o...
Source: Comparative Clinical Pathology - October 29, 2019 Category: Pathology Source Type: research