Renoprotective effect of Otostegia persica on vancomycin-induced nephrotoxicity in rats
AbstractVancomycin (VCM), as a glycopeptide antibiotic, is commonly used against serious and life-threatening bacterial infections. Nephrotoxicity is the most important complication of VCM in which free radicals play an important role in   its pathophysiology. Thus, the aim of this research was to examine effects of hydro-alcoholic extract ofOtostegia persica on nephrotoxicity of VCM in rats. Thirty male Wistar rats were at random divided into 5 groups. The control group was given normal saline. The other groups were treated every 12 h for 7 consecutive days with VCM (200 mg/kg i.p.) alone, VCM (200 mg/kg i.p.) plusOt...
Source: Comparative Clinical Pathology - April 3, 2021 Category: Pathology Source Type: research

Protective impacts of gallic acid against cadmium-induced oxidative toxicity in the ovary of rats
This study evaluates the probable effects of induced reproductive toxicity by cadmium and its amelioration by gallic acid (GA). Adult female rats were daily administered with cadmium (CdCl2) (5 mg/kg) singly or combined with GA (20 mg/kg) for 14 days to evaluate the effect on antioxidant status and ovary function using biochemical and histological analysis. Cadmium-treated rats alone revealed a considerable increment in total body weight and ovaries ’ weight due to muscle wasting. However, GA + cadmium reversed considerably the tread and offered protection against cadmium-induced muscle wasting. Cadmium treatment alo...
Source: Comparative Clinical Pathology - April 2, 2021 Category: Pathology Source Type: research

Comparative studies on ovarian hormones and hematobiochemical profile of lactating and non-lactating she-camels ( Camelus dromedarius )
AbstractDue to the lack of reports on the lactation stage effect in she-camels, this study aimed to study the physiological changes in the hematological and biochemical profiles of she-camels during the lactation period. Twenty-four she-camels with bodyweights 350 –550 kg were divided into two equal groups lactating and non-lactating she-camels (control she-camels). Blood samples were collected through a jugular vein with and without anticoagulants. The findings revealed a significant decrease (P
Source: Comparative Clinical Pathology - March 12, 2021 Category: Pathology Source Type: research

Biochemical and hormonal studies of metoclopramide-induced hyperprolactinemic female rats administered ethanol Leaf Extract of Kigelia africana
AbstractTo study the biochemical and hormonal parameters in metoclopramide-induced hyperprolactinemic female Wistar rats administered ethanol leaf extract ofKigelia africana. Thirty (30) female Wistar rats were grouped into two major parts: (i) normal control (n = 5) and (ii) experimental (n = 25) to induce hyperprolactinemia. The experimental group was divided into five groups of five animals each, given a total of six in all. Group 1 received the distilled water alone, group 2 was metoclopramide-induced hyperprolactinemic rats only, group 3 comprised metoclopramide-induced hyperprolactinemic rats administered bromocripti...
Source: Comparative Clinical Pathology - March 10, 2021 Category: Pathology Source Type: research

In vitro cytotoxicity and in vivo sub-acute toxicity studies on Vitex doniana (Verbenaceae) leaves extract in MCF-7 breast cancer cells and female Wistar rats
This study examined the effects ofV. doniana extract on the growth of MCF-7 breast cancer cells, B16-F10 melanoma cells and sEnd.2 endothelial cells. As well, the study investigated the effects of the extract on breast cancer cell morphology, cell cycle progression, migration and invasion, and levels of apoptosis regulatory proteins (Bad, PARP and p53). In vivo, the sub-acute toxicity of the extract in female Wistar rats was examined. The effects ofV. doniana extract on MCF-7 cell growth, cell morphology and cell cycle were studied using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, light mi...
Source: Comparative Clinical Pathology - March 8, 2021 Category: Pathology Source Type: research

Monitoring of cardiac biomarkers and vitamin D concentration in response to calcium infusion in Darehshori horses
AbstractCalcium is an essential mineral and plays a direct role on cardiac muscle activities when given intravenously. The aim of the present study was to determine the effects of calcium infusion on serum cardiac biomarkers and vitamin D concentration in Darehshori horses. Calcium infusion was made once a week, and blood samples were taken before and 1 h after to determine serum cardiac troponin I, creatine kinase myocardial band (CK-MB), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), calcium, and vitamin D changes up to four consecutive weeks. Increases in blood calcium along with suppression of serum vitamin D...
Source: Comparative Clinical Pathology - March 4, 2021 Category: Pathology Source Type: research

Synovial fluid analysis of MMP-2, MMP-9, and COX-2 as diagnostic markers for naturally occurring septic and aseptic arthritis in horses
In conclusion, the results confirm that MMP-9 and COX-2 detection offers an important diagnostic potential for septic and aseptic arthritis in horses. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 4, 2021 Category: Pathology Source Type: research

Extracellular matrix interaction and apoptosis of the epididymal epithelium following hypothyroidism in Balb/C mouse
This study was designed to characterize the effects of hypothyroidism on epididymis ’ structure. Twenty Balb/C mice were randomly divided into control, and hypothyroid group which received 0.05% 6-n-propyl-2-thiouracil (PTU) for 35 days. At the end of the experiment, to confirm the hypothyroidism, T4 and thyroid-stimulating hormone (TSH) levels were measured using chemiluminescen t immunoassay kit. Real-time PCR, immunohistochemistry, periodic acid-Schiff (PAS) staining, TUNEL assay, and biochemical measurements were carried out. Based on the results, T4 level was significantly lower in the hypothyroid group compared...
Source: Comparative Clinical Pathology - March 1, 2021 Category: Pathology Source Type: research

Comparative study of thyroid hormones in camels at different physiological stages and pathological conditions and treatment trial for congenital goiter
AbstractHypothyroidism and iodine deficient disorders (IDD) are global problems affecting wide range of species. The camel is sensitive to iodine. This work was conducted to monitor thyroid hormones in group of camels at different physiological and pathological status. One hundred camels randomly selected were allocated into five groups including racing, late pregnant, lactating, aborted, and young calved. Four calves with congenital goiter (CCG) along with their mothers (MCCG) were also included. The affected calves were treated with two I/V doses of sodium iodide 1 week apart. T4 was highest in healthy calves followed by...
Source: Comparative Clinical Pathology - February 26, 2021 Category: Pathology Source Type: research

Evaluation of hematological variables in layer pullets after vaccination and challenge with E. coli
AbstractAvian colibacillosis is the most common bacterial disease in poultry. Vaccination has been extensively used in order to protect birds from colibacillosis infection. The aims of this study were to assess hematological alterations in commercial layer pullets after intratracheal challenge with a pathogenic strain ofEscherichia coli as well as to investigate the differences observed between birds vaccinated with different vaccination schemes against avian colibacillosis. Four groups of conventional pullets were reared in the same house following different vaccination protocols against colibacillosis. Birds in group A r...
Source: Comparative Clinical Pathology - February 22, 2021 Category: Pathology Source Type: research

Thrombocytes of diploid and triploid rainbow trouts assessed by flow cytometry and aggregation assay
In conclusion, triploid thrombocytes have lower aggregation capacity than the diploid ones. This response is possibly related to the deficiency of receptors on the surface of thrombocytes of triploid trouts, which requires further studies for confirmation. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - February 17, 2021 Category: Pathology Source Type: research

Attenuation of diabetic nephropathy in alloxan-induced diabetic rats by Solanum macrocarpon Linn aqueous leaves extract
AbstractSolanum macrocarpon leaf is one of the medicinal plants locally used to manage diabetic nephropathy. This has been established in scientific studies as one of the main factors responsible for kidney damage in diabetes mellitus patients. Hence, our study investigated the attenuation of diabetic nephropathy in alloxan-induced diabetic rats usingSolanum macrocarpon Linn aqueous extract leaves. A total of 48 male Wistar rats of 7 to 8 weeks old were used in this study. A single injection of alloxan (150 mg/kg, i.p.) was used to induce diabetes in rats. Induced diabetic rats were then administered different doses (12.45...
Source: Comparative Clinical Pathology - February 17, 2021 Category: Pathology Source Type: research

Effects of dietary supplementation with glutamine and glutamate on the recovery of bitches after ovariohysterectomy due to pyometra
AbstractPyometra is a uterine infection that triggers intense catabolism, involving glutamine. We aimed to investigate the effects of supplementation with L-glutamine and L-glutamic acid for 21 days in the preoperative and postoperative outcomes of bitches that underwent ovariohysterectomy (OHE) due to pyometra. Fourteen bitches with pyometra were randomly assigned into two groups after OHE: the supplemented group (G-GLN,n = 9) and control group (G-CON,n = 5). Body score data and venous blood samples were collected. Hematological parameters, serum concentrations of glutamine (Gln), glutamate, alanine (Ala), and other energ...
Source: Comparative Clinical Pathology - February 17, 2021 Category: Pathology Source Type: research

Chenopodium quinoa ethanolic extract ameliorates cyclophosphamide ®-induced hepatotoxicity in male rats
This study aimed to investigate ameliorative effect ofChenopodium quinoa ethanolic extract (QEE) against cyclophosphamide ®-induced hepatotoxicity. Four groups (10 male Wistar rats each) were used: (1) healthy control group; (2) rats treated orally with QEE (400 mg/kg/day) for 4 weeks; (3) rats injected intraperitoneally with cyclophosphamide® (150 mg/kg/week) for 4 weeks; (4) rats received QEE after cyclophosphamide ® intoxication another 4 weeks. The results revealed that QEE succeeded to decrease the hepatotoxicity-induced by cyclophosphamide®; this was evidenced by the significant reduction in seru...
Source: Comparative Clinical Pathology - February 15, 2021 Category: Pathology Source Type: research

Berberine mitigates diabetes-induced erectile dysfunction in rats through modulation of antioxidant status and critical enzyme activity
AbstractThe study evaluated the effects of berberine on sexual activity and crucial biomolecules linked to penile function in diabetic rats. Sixty-three (63) adult male rats were used and divided into nine groups (n = 7). Group I received 0.1 M citrate buffer at pH 4.5 orally as normal control. Group II received 5.0 mg/kg sildenafil citrate orally. Groups III and IV rats received 50.0 mg/kg and 100.0 mg/kg of berberine orally, respectively. Group V rats were induced with STZ intraperitonially (50 mg/kg b.w.t.) (diabetic control rats). Group VI diabetic erectile dysfunctional (DED) rats received sildenafil citrate orally. G...
Source: Comparative Clinical Pathology - February 15, 2021 Category: Pathology Source Type: research

Evaluation of the difference between feline immunodeficiency virus (FIV)-infected and healthy cats by using clinicopathological parameters, oxidative stress, and total antioxidant power
The objective of this study was to compare physical examination and hematological parameters, oxidative stress, and total antioxidant power between FIV-infected and healthy cats. Cats were evaluated for FIV infection by an immunochromatographic assay. The study was carried out on 20 FIV-infected cats and 27 clinically healthy mixed-breed domestic cats. Body condition score, body weight, mucous membrane color score, vital signs, hematological parameters, oxidative stress, and total antioxidant power were examined. These parameters were compared between FIV-infected and healthy cats by using thet test (normal distribution da...
Source: Comparative Clinical Pathology - February 15, 2021 Category: Pathology Source Type: research

Protein and electrolyte contents and histopathology of longus colli muscle in cachexia of Red Bororo beef cows
This study evaluated the protein and electrolyte contents and histopathology of longus colli (neck chain) muscle in cachectic Red Bororo (RB) beef cows. Body condition score (BCS 1 –9) was the basis for selection of slaughter cows in a case control study. At antemortem inspection, cachectic (n=39) and control (n=15) cows had BCS of 1 –2 and ≥ 5, respectively. Longus colli muscle was collected after slaughter on ice pack. Protein and electrolyte (sodium, potassium, calcium, chloride, bicarbonate, inorganic phosphate ions) contents in aqueous supernatant of the muscle homogenate were estimated. Stained tissue ...
Source: Comparative Clinical Pathology - February 12, 2021 Category: Pathology Source Type: research

Alleviating effects of Lavandula aqueous extract on asthmatic complications in a mouse model
AbstractAsthma is an inflammatory disease of the respiratory system affecting over 300 million people worldwide.Lavandula has been traditionally used to treat inflammatory diseases and has also several medical applications. We aimed to explore the anti-inflammatory effects ofLavandula extract on a murine model of asthma. Twenty-eight Balb/c mice were divided into four groups of seven animals. The negative control group was treated with Phosphate-buffered saline, and the remaining three groups received ovalbumin to induce asthma. Subsequently, two asthmatic groups received budesonide or Lavandula extract. Afterward, the cha...
Source: Comparative Clinical Pathology - February 11, 2021 Category: Pathology Source Type: research

Evaluating the effect of hydro-alcoholic extract of Phoenix dactylifera L . spathe on streptozotocin-induced diabetic rats
AbstractPhoenix dactylifera L. (Arecaceae) spathe has antioxidant and anti-inflammatory effects. Effects of hydro-alcoholic extract ofPhoenix dactylifera spathe were examined in streptozotocin (STZ)-induced diabetic rats. Forty-nine male Wistar rats were randomly divided into seven groups; group 1 received normal saline solution daily, group 2 received STZ (50 mg/kg) on the first day, group 3 received STZ (50 mg/kg, ip) at the beginning of the study and metformin (150 mg/kg), daily for 30 days, groups 4 –6 received STZ (50 mg/kg, ip) at the beginning of the study and were treated withPhoenix dactylifera spathe extrac...
Source: Comparative Clinical Pathology - February 10, 2021 Category: Pathology Source Type: research

Synergistic erythropoietic mechanisms of Chromolaena odorata and Tithonia diversifolia in the bone marrow of Wistar rats
This study aimed to elucidate the scientific mechanisms that underpin the traditional use ofChromolaena odorata andTithonia diversifolia as remedy for paediatric anaemia. We investigated the impact of these plants on erythropoietin and erythropoietin receptor expression and monitored cyclin-dependent kinase inhibitors, and FasL/FAS signalling mechanism therefrom. Three samples were prepared from spouting leaves ofC. odorata and leaves of floweringT. diversifolia as follows: C.O-group (100gC. odorata was blended in 100 ml distilled/deionized water (dd-water)), T.D-group (100gT. diversifolia was blended in 100 ml dd-water) a...
Source: Comparative Clinical Pathology - February 10, 2021 Category: Pathology Source Type: research

Oxidative biomarkers and lipid alterations in euthyroid and hypothyroid dogs
AbstractIn recent years, few reports suggest that the oxidative process may be present in hypothyroid patients; however, these reports were mostly contradictory and with very few data regarding canine patients. So, this investigation was designed to study oxidative stress alterations in both hypothyroid and euthyroid dogs. The study was conducted on thirteen dogs (six hypothyroid dogs and seven euthyroid dogs) with an age range of 4 –7 years. Establishing hypothyroidism was based on clinical signs and results of thyroid hormones evaluation. Serum samples were taken from each animal for evaluation of free T4 (fT4...
Source: Comparative Clinical Pathology - February 7, 2021 Category: Pathology Source Type: research

Interleukin-1 β, tumor necrosis factor-α, and oxidative stress biomarkers in cows with acute Brucella abortus infection
AbstractThe aim of this study was to investigate the possible alterations in some cytokines and oxidative stress biomarkers in cows with acuteBrucella abortus (B. abortus) infection. The study has been carried out on twenty-two cows ’ sera. All serum samples were examined for brucellosis by indirect enzyme-linked immunosorbant assay (i-ELISA). Cows that were seronegative forB. abortus infection were used as a control group; meanwhile, cows that were diagnosed positive forB. abortus infection were used as an infected group. Cytokine profile and oxidative state markers showed significant increases in interleukin-1 &bet...
Source: Comparative Clinical Pathology - February 3, 2021 Category: Pathology Source Type: research

Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique
The objective of this study is to identify macroscopic and histopathological lung lesions in slaughtered sheep during the period from December 2018 to December 2019 and to determine the presence ofBovine parainfluenza 3 virus (BPI3V) in frozen sheep pneumonic lung using a direct immunofluorescence antibody technique (DFAT). The overall prevalence of lung affection was 11% (1440/13084). The gross lesions were acute bronchopneumonia (58.12%), interstitial pneumonia (07.15%), fibrinous bronchopneumonia (10.70%), suppurative bronchopneumonia (03.47%), verminous pneumonia (13.75%), and ovine pulmonary adenomatosis (06.81%). The...
Source: Comparative Clinical Pathology - February 3, 2021 Category: Pathology Source Type: research

Intranasal diffuse large B-cell lymphoma of immunoblastic type with extensive osteolysis in a dog
This report describes a rare case of nasal lymphoma in a dog. A stray dog was presented with a swollen snout showing ulcerations and sanguinopurulent discharges. Cytological evaluation of fine-needle aspiration specimens revealed discrete round cell tumor presumed as B-cell lymphoma. Histopathological examination and immunohistochemistry staining of biopsy confirmed diffuse large B-cell lymphoma of immunoblastic type. This case is one of the rare reports of nasal lymphoma in dogs and the first immunoblastic type. Our finding emphasizes the significance of considering lymphoma in differential diagnosis of nasal tumors in do...
Source: Comparative Clinical Pathology - February 2, 2021 Category: Pathology Source Type: research

Safety assessment and antimalarial property of methanol extract of Fagara zanthoxyloides root-bark on Plasmodium berghei -infected mice
This study investigated the safety profile and antimalarial activity of methanol extract ofFagara zanthoxyloides root-bark onPlasmodium berghei-infected mice. Phytochemical analysis and acute toxicity (LD50) of MEFZRB were determined using standard methods. Thirty-six Swiss mice (28 –34 g) were distributed into 6 groups (n = 6). Groups 1 and 2 served as normal and negative control respectively. Group 3 mice were parasitized and treated with 5 mg/kg body weight of Coartem® (positive control). Groups 4–6 wereP. berghei infected and orally treated with 200, 400, and 600  mg/kg body...
Source: Comparative Clinical Pathology - February 1, 2021 Category: Pathology Source Type: research

Bioactive Moringa olifera seed extracts attenuates cholesterol gall stones in hyperglycaemic Swiss mice
In this study, we report the gall bladder cytoprotective ability ofMoringa oleifera seed extracts and the hepatoprotective potential of the seed cake in hyperglycemic and cholesterol gall stone-induced Swiss mice. We show that the seed cake contains 37% protein and caused a 40% increase in body weight over 5  weeks, but the seed oil caused only a 15% increase in body weight over the same period, which supports the possible use of the seed oil as a functional food for weight loss. Seed cake treatment presented the potential for glycemic control, improved hematological and lipid profile and enhanced live r antioxidant a...
Source: Comparative Clinical Pathology - January 30, 2021 Category: Pathology Source Type: research

Critical roles of TLRs on the polarization of mesenchymal stem cells for cell therapy of viral infections: a notice for COVID-19 treatment
AbstractMesenchymal stem cells (MSCs), as one of the leading cell-based therapy, have provided a strong link between clinical investigation and basic research. MSCs have been successfully employed in treating graft versus host disease (GvHD), autoimmune disease, and several other diseases, particularly with high immune activity. Recently, MSCs have attracted attention to treating untreatable viral infections such as severe coronavirus disease 2019 (COVID-19). Given that the Toll-like receptors (TLRs) are directly able to detect internal and external hazard signals, and their stimulation has an intense effect on the ability...
Source: Comparative Clinical Pathology - January 29, 2021 Category: Pathology Source Type: research

Haematological profiles of indigenous Boschveld chickens on probiotic-yeast ( Saccharomyces cerevisiae ) supplementation
AbstractHaematological characteristics of Boschveld chickens fed baker ’s yeast (Saccharomyces cerevisiae) –supplemented diets were investigated. Six hundred, day-old unsexed Boschveld chicks were divided into six equal groups and each group replicated 5 times. Birds aged 1 to 49 days were offered starter diet, whereas those aged 50–91days were offered grower diet. Birds in the control group (T1) rec eived basal diet without yeast, while those in groups T2, T2, T3, T4, T5 and T6 were offered basal diet supplemented with yeast at 2.5, 5.0, 7.5, 10.0 and 12.5 g/kg feed, respectively, in a completely randomi...
Source: Comparative Clinical Pathology - January 28, 2021 Category: Pathology Source Type: research

The diagnostic value of circulating microRNA-499 versus high-sensitivity cardiac troponin T in early diagnosis of ST segment elevation myocardial infarction
This study was conducted on 100 Egyptian patients who presented during the first 3 h of AMI in the emergency department and 98 healthy volunteers as a control group. Serum samples for both groups were analyzed using reverse transcription and real-time PCR by TaqMan ® MicroRNA assays.ResultsThe concentration of miRNA-499 was significantly elevated in STEMI patients compared to healthy controls (p
Source: Comparative Clinical Pathology - January 26, 2021 Category: Pathology Source Type: research

Effects of Nauclea latifolia and Uvaria afzelii stem bark extracts on nitric oxide and biomolecules in platelets of complete Freund adjuvant-induced arthritic female rats
This study investigated the effect of African peach (AP) andUvaria afzelii (UA) extracts on arginase, nitric oxide (NO), angiotensin-1 converting enzyme (ACE), and cholinergic and purinergic enzymes in platelets of complete Freund adjuvant (CFA)-induced arthritic rat model to explain a possible anti-arthritic mechanism of extracts. Sixty adult Wistar female rats (140 –280 g) were divided into twelve groups (n = 5). Group I received oral administration of 0.9% NaCl; group II received 10 mg/kg of indomethacin; group III and IV rats received oral administration of 150 and 300 mg/kg of AP extract, respectively; and group...
Source: Comparative Clinical Pathology - January 25, 2021 Category: Pathology Source Type: research

Evaluation of hemato-biochemical indices and oxidative stress in goats: a potential risk towards arsenic toxicity in contaminated zone of West Bengal, India
AbstractArsenic is one of the widely dispersed elements in the Earth ’s crust and exists in the environment due to natural and anthropogenic sources. The toxin is greatly expanded through intensive application of groundwater in agriculture in the region within the Green Revolution framework. It is an environmental chemical element of high concern for human and anim al health. Agricultural land is constantly contaminated with arsenic due to application of groundwater for irrigation purposes. Animals, being exposed to arsenic through contaminated drinking water, exhibit major abnormalities in the blood and tissues of a...
Source: Comparative Clinical Pathology - January 25, 2021 Category: Pathology Source Type: research

Cannabis sativa L. Mitigates Oxidative Stress and Cholinergic Dysfunction; and Modulates Carbohydrate Metabolic Perturbation in Oxidative Testicular Injury
AbstractThe therapeutic potentials ofCannabis sativa L. on oxidative-induced testicular dysfunction were investigated in isolated testes of male albino rats.C. sativa leaves were subjected to sequential extraction to yield the hexane, dichloromethane (DCM), and ethanol extracts. Oxidative injury was induced by incubating testicular tissue homogenate with 0.1 mM FeSO4 for 30 min at 37 °C. Treatment was done by incubating the tissues withC. sativa extracts and 0.1 mM FeSO4 under same conditions. Induction of oxidative injury led to significant (p
Source: Comparative Clinical Pathology - January 25, 2021 Category: Pathology Source Type: research

Serum protein capillary electrophoretic pattern during the neonatal period in dairy calves
The objective of this study was to characterize serum protein capillary electrophoretic pattern in 20 clinically healthy neonate calves. The neonates were monitored from birth up to 28  days of age. Venous blood samples were collected from the jugular vein and used for the determination of serum protein fractions using capillary electrophoresis technique. The younger calves 4–11 days old showed electrophoretic pattern composed of albumin and α1-, α2-, β1-, β2- and γ-globulins, whereas the young calves 15–28 days old showed a pattern composed of albumin and α1-, &...
Source: Comparative Clinical Pathology - January 23, 2021 Category: Pathology Source Type: research

Serum calcium determination with atomic absorption spectrophotometry and colorimetric method: association and predictive equations between them in cattle and sheep samples
The objectives of the present study were to compare the serum calcium (Ca) concentrations in cattle and sheep measured by the flame atomic absorption spectrophotometry (AAS) and those obtained with a colorimetric method (CM) and to generate equations that allow their prediction. Serum Ca concentration was determined in 50 dairy cattle and 50 dairy sheep non-hemolyzed blood samples with AAS and CM. The data were analyzed with paired samplet test and Bland –Altman plots were generated. Using a random number generator, 25 samples from each species were selected to create predictive equations between the two methods usin...
Source: Comparative Clinical Pathology - January 23, 2021 Category: Pathology Source Type: research

Correction to: Biochemical applications of Kigelia africana (Lam.) Benth. fruit extracts in diabetes mellitus
The Correction to this paper has been published:https://doi.org/10.1007/s00580-020-03185-x (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 23, 2021 Category: Pathology Source Type: research

Effects of citral on serum inflammatory factors and liver gene expression of IL-6 and TNF-alpha in experimental diabetes
In this study, the effects of citral on reducing inflammation in experimental diabetes in rats were investigated. Forty rats were randomly divided into four groups. There were two control groups (healthy controls (H) and citral alone-treated control (HC)) and two diabetic groups (diabetes (D) and diabetes+citral treatment (DC)). After diabetes confirmation on day 7, treatment with citral (300 mg/kg) was started for 2 weeks by gavage in the DC and HC groups. On days 0, 7, and 21 of the study, inflammatory elements of blood serum, IL-6, TNF- α, haptoglobin, and α2-macroglobulin were compared between the four grou...
Source: Comparative Clinical Pathology - January 22, 2021 Category: Pathology Source Type: research

Correlation between some haematological parameters, leukocyte ratios, parasitaemia and their prognostic value in canine trypanosomosis
AbstractAfrican trypanosomosis is a disease of high economic importance in animals and humans caused by an apicomplexan haemoparasite of the genusTrypanosoma. Canine trypanosomosis is relatively common in Nigeria because of the high prevalence of the insect vector. The haematology, erythrocyte, and serum sialic acid levels of dogs experimentally infected with Federe strain ofTrypanosoma brucei brucei was studied in a total of five (5) 4-month old dogs. Three of the dogs were randomly selected and inoculated subcutaneously with 1 × 106 ofT. brucei, while the remaining two dogs served as the uninfected control. The par...
Source: Comparative Clinical Pathology - January 21, 2021 Category: Pathology Source Type: research

Risk factors associated with the seroprevalence of caseous lymphadenitis in sheep
This study aimed to determine the prevalence of caseous lymphadenitis (CLA) caused byCorynebacterium pseudotuberculosis (C. Pseudotuberculosis) and its risk factors of sheep reared in four districts (Sherbin, Mansoura, Belkas, and Dekernes) in Dakahlia Governorate. A total of 346 sheep serum samples collected from the study area were examined against antibodies forC. Pseudotuberculosis infection using enzyme-linked immunosorbent assay (ELISA). The prevalence ofC. Pseudotuberculosis infection was estimated at 73.98%. The highest prevalence was found among flocks reared in Sherbin (83.65%) followed by those in Mansoura (74.4...
Source: Comparative Clinical Pathology - January 21, 2021 Category: Pathology Source Type: research

Ameliorative effects of Garcinia hydroxybiflavanonol 1 (GB1) isolated from Garcinia kola seeds on cadmium chloride (CdCl 2 ) induced reproductive toxicity in the testis of the male Wistar rats
This study is aimed at investigating the ameliorative effects of administration of (GB1) on the reproductive health of cadmium chloride (CdCl2) –intoxicated male Wistar rats. Extraction, fractionation and isolation of Garcinia hydroxybiflavanonol (GB 1) were done from seeds ofGarcinia kola. Thirty-six male adult Wistar rats weighing 170 –190 g were acclimatized for 2 weeks and randomly divided into three groups. Group A were given distilled water as control, group B 2.5 mg/kg b.w. of CdCl2 and group C 2.5  mg/kg b.w. of CdCl2 and 2  mg/kg b.w. of GB1 dissolved in Tween20. The rats were orall...
Source: Comparative Clinical Pathology - January 20, 2021 Category: Pathology Source Type: research

Some clinical and laboratory aspects following oral administration of Citrullus colocynthis in Baluchi sheep
In this study, the effects of different parts ofCitrullus colocynthis including fruit, leaf, and fruit and leaf mixture on clinical, hematological, and biochemical parameters of sheep blood serum were investigated. Twenty male Baluchi sheep of about 1 year old were admitted to the study. The animals were divided into three treatment groups and one control group and received fruit, leaf, and fruit and leaf mixture ofCitrullus colocynthis with a dose of 0.25  g/kg for 1 month. Repeated measures ANOVA statistical test by using SPSS statistical software was used to compare the results between groups. The results showed th...
Source: Comparative Clinical Pathology - January 18, 2021 Category: Pathology Source Type: research

Nephroprotective role and antioxidant capacity of Atriplex halimus on carbon tetrachloride-induced kidney damage in rats
AbstractThe aim of this work was to study the nephroprotective role and antioxidant capacity ofAtriplex halimus aqueous leaves extract (AHAE) against carbon tetrachloride-induced kidney injury in rats. The experimental rats were randomized into four experimental groups of eight each: Group (C) served as a control received a normal diet, Group (CCl4) received a normal diet for 4  weeks and then was injected with one dose of 1.0 ml/kg body weight of carbon tetrachloride dissolved in olive oil (v/v) intraperitoneally, Group (AHAE + CCl4) received 200  mg/kg body weight of AHAE for 4 weeks and then was inje...
Source: Comparative Clinical Pathology - January 16, 2021 Category: Pathology Source Type: research

Chemoprotective effects of Allium cepa and Allium sativum on lead acetate –mediated oxidative stress and dyslipidemia in Wistar rats
In conclusion,Allium cepa andAllium sativum mitigated oxidative stress and dyslipidemia in lead acetate –exposed Wistar rats, and thus, suggests protective effects on cardiovascular diseases. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 12, 2021 Category: Pathology Source Type: research

Immunohistochemical expression of stem cell markers ALDH1 and CD44 in urothelial carcinoma of the urinary bladder
This study aimed to study ALDH1A1 and CD44 expression among urothelial cancer patients. Sixty cases of urothelial carcinoma were studied using ALDH1A1 and CD44 immunohistochemistry, and their expression was correlated with different clinicopathologic parameters. There was only a statistically significant relationship between presence of bilharziasis and expression of ALDH1. No statistically significant relationship is found between ALDH1 or CD44 expression and age, gender, tumor grade, histological variants of urothelial carcinoma, depth of invasion (T), presence of carcinoma in situ in adjacent mucosa, lymphovascular inva...
Source: Comparative Clinical Pathology - January 9, 2021 Category: Pathology Source Type: research

Nephro-protective efficacy of Blighia sapida stem bark ether fractions on experimentally induced diabetes nephropathy
AbstractStudies suggest thatBlighia sapida (B. sapida) possesses hypoglycemic potential. The potentials ofB. sapida to ameliorate diabetic complications including diabetic nephropathy are yet to be established. This present research therefore investigated the role ofB. sapida in renal functions of STZ-induced diabetes rats. Petroleum and diethyl ether fractions ofB. sapida ’s (PEFBS and DEFBS) stem bark were used in this study. Diabetes was induced by intraperitoneal injection of 50 mg/kg b. w. of STZ singly. Short-term effects of PEFBS and DEFBS at 100 mg/kg and 200 mg/kg b. w. and metformin (5 mg/kg b.w) were studi...
Source: Comparative Clinical Pathology - January 7, 2021 Category: Pathology Source Type: research

Nutritional secondary hyperparathyroidism –induced facial osteodystrophy in a Labrador puppy
This report describes unusual presentation of osteodystrophy causing facial hyperostosis due to nutritional secondary hyperparathyroidism in a puppy. A female Labrador Retriever dog aged 5  months was presented with progressive bilaterally symmetrical swelling on facial region, hypersalivation, and reduced growth rate. Excessive meat intake in daily ration since weaning was reported. Clinical examination revealed dyspnoea, open mouth breathing, epiphora, excessive nasal secretions, t achycardia, pale mucous membranes, debility, loosely attached teeth, and pigmented oral mucosa of the upper jaw. Microcytic anemia, lymp...
Source: Comparative Clinical Pathology - January 7, 2021 Category: Pathology Source Type: research

Clinical and laboratory findings in barren Arabian mares
In conclusion, endometritis was the common clinical findings in barren Arabian mares in Saudi Arabia, whileE. coli was the frequent isolated bacteria. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - January 6, 2021 Category: Pathology Source Type: research

Pathological changes in the mucous membrane of the small intestine due to prolonged consumption of palm oil
AbstractAlthough palm oil is a very widely consumed commodity, health concerns have arisen regarding its safety over the past few decades, due to its high saturated palmitic acid content. The aim of this experimental study was to evaluate the effect of prolonged palm oil consumption on the morphology and function of the small intestine (SI) as well as features of proliferation in these cells. Ten WAG male rats, separated randomly into two equal groups, were given 10 g/kg of sunflower oil (control group) and palm oil (main group), respectively, in their food rations for 5 months. Microscopic investigation and morphometric s...
Source: Comparative Clinical Pathology - January 5, 2021 Category: Pathology Source Type: research

Modification of erythrogram, testosterone and prolactin profiles in jacks following experimental infection with Trypanosoma congolense
AbstractEquine trypanosomosis, a haemoparasitic disease leads to anaemia, low testosterone and prolactin levels. The aim of this study was to examine the effect of experimentalTrypanosoma congolense (T. congolense) infection in jacks on erythrogram, testosterone and prolactin profiles. Four apparently healthy jacks aged 6 –7 years, housed in fly-proof stables, were used for this study. To establish infection, they were, each, intravenously inoculated with 2 ml of blood from infected donor jack containing 2.0 × 106 trypanosomes. Daily rectal temperatures were measured, for 2 weeks pre-infection and 4 weeks post-...
Source: Comparative Clinical Pathology - January 3, 2021 Category: Pathology Source Type: research

Effects of n-hexane and ethylacetate fractions of Terminalia catappa leaf extract in experimental Trypanosoma brucei brucei infection in Wistar rats
AbstractDue to the toxic effects from using conventional trypanocidal drugs, there is need for development of safer alternatives to combat trypanosomosis. The aim of this study was to evaluate the effects of n-hexane and ethylacetate fractions ofTerminalia catappa leaf extract inWistar rats experimentally infected withTrypanosoma brucei brucei. Forty-five (45) adultWistar rats were divided into 9 groups (A –I) comprising of 5 rats each after acclimatisation for 7 days. Groups A-H were infected intraperitoneally withT. brucei brucei (day 0), treatment given to groups A –G at day 3 post-infection while group...
Source: Comparative Clinical Pathology - January 3, 2021 Category: Pathology Source Type: research

Evaluation of lead-induced cardiac toxicity in mice by measurement of selected biochemical as well as oxidative indices
AbstractRegarding the potential interrelation between lead (Pb) toxicity and cardiovascular diseases, the current investigation aims to study the alterations of some metabolic enzyme activities as well as oxidative stress-related parameters in the myocardium of lead-exposed mice. Twelve male mice are distributed arbitrarily into two equal groups. First group served as control. Mice in group 2 received water containing 1000 mg L−1 lead acetate. The results revealed that Pb-treated mice had significantly higher values of cardiac troponin I and creatine kinase MB as compared with the control group. Moreover, Pb-treated ...
Source: Comparative Clinical Pathology - November 11, 2020 Category: Pathology Source Type: research