Gene expression of hemostasis biomarkers following HIIT and RIPC
The objective of this study was to examine how these strategies might affect gene expression of hemostasis biomarkers in middle-aged male Wistar rats. Twenty-four male Wistar rats (12-month-old) were randomly assigned to four groups: high-intensity interval training (HIIT), remote ischemic preconditioning +HIIT (RIPC+HIIT), sham-HIIT (S-HIIT), sham-RIPC+HIIT (S-RIPC+HIIT). The experimental groups followed specific exercise protocols three times per week. The HIIT involved a 5-min warm-up period at 40–50% vVO2max, followed by 6 sets of 2-min high-intensity exercise at 85 –90% vVO2max, interspersed by 5-min inactive r...
Source: Comparative Clinical Pathology - February 6, 2024 Category: Pathology Source Type: research
Modulation of PON-1 by acetate reverses hepatic dysmetabolism in experimentally induced PCOS
AbstractPolycystic ovarian syndrome (PCOS) is well documented as the leading cause of infertility globally, and its long-term complications include non-alcoholic fatty liver disease (NAFLD) which results in a cascade of events leading to liver cirrhosis as well as hepatic carcinoma. Paraoxonase-1 (PON-1) is an antiatherosclerotic component, which protects against hepatic injury. Short-chain fatty acids (SCFAs), particularly acetate, are synthesized by the gut microbiota and play a central role in metabolic regulation. The present study investigated the impact of acetate on PCOS-associated hepatic dysregulation and probable...
Source: Comparative Clinical Pathology - February 6, 2024 Category: Pathology Source Type: research
Inclusion of essential oils in a calf milk replacer and their effects on growth performance and the immune and oxidative systems
This study determined whether a reformulated milk replacer containing a blend of essential oils composed of cinnamon, oregano, and eucalyptus improves suckling calves ’ immune and oxidative systems, consequently improving productive performance. Sixteen Holstein calves (ten days old) suckled for 60 days. The animals were divided into a control group (n = 8), which received the commercial milk replacer, and a treatment group (n = 8), which received a milk replacer containing oils. The animals were fed twice daily with 0.25 kg of milk replacer diluted in two liters of water at each feeding. The milk replacer in t...
Source: Comparative Clinical Pathology - February 5, 2024 Category: Pathology Source Type: research
Effect of Pentaclethra macrophyla Benth. Leaf on expression of P53, IRS, HsD17 β2, FTO, and CYP11a genes in letrozole-induced polycystic ovarian syndrome rats
AbstractHyperandrogenism and menstruation difficulties are the hallmarks of polycystic ovarian syndrome (PCOS), a disorder affecting women in their reproductive years. The purpose of this study is to determine ifPentaclethra macrophylla leaf extract is beneficial in treating letrozole-induced PCOS rats. To ascertain the antioxidant characteristics of the plant extract, in vitro tests including the hydroxyl radical scavenging assay, FRAP, DPPH radical scavenging assay, and iron chelating assay were performed. A toxicology study was also conducted. Following PCOS induction, the standard and plant extract groups received trea...
Source: Comparative Clinical Pathology - February 5, 2024 Category: Pathology Source Type: research
Spermicidal and antioxidant potency of Solanum macrocarpon L. (African eggplant) leaf ethanol extract in albino rats
AbstractOxidative processes give rise to reactive oxygen species/oxidants which are detrimental to living cells and underlie many pathologic conditions. On the other hand, many plant species possess antioxidant properties and phytochemicals which are closely linked to their therapeutic potentials.Solanum macrocarpon is a tropical plant which is traditionally claimed to possess therapeutic properties. Although, antioxidant potentials have been reported for differentSolanum species and antioxidants have been reported to improve sperm morphology and motility; this has not been fully elucidated inS. macrocarpon. Following a ...
Source: Comparative Clinical Pathology - February 3, 2024 Category: Pathology Source Type: research
The influence of season and sex on rumen fluid and hematobiochemical constituents of Alpacas (Vicugna pacos) in Egypt
AbstractAlpaca (Vicugna pacos) is a South American camelid that was introduced recently to Egypt and considered a zoo animal in different country zoos. There are no available data regarding the normal values of rumen fluid and hematobiochemical constituents of alpacas bred in Egypt. The aim of this study was to estimate normal values for rumen fluid and blood constituents of alpaca and determine the influence of season and sex on these parameters under Egyptian circumstances. The study was conducted on seventeen (8 female and 9 male); 2–6 years apparently healthy alpacas. Rumen fluid and blood samples were taken from ...
Source: Comparative Clinical Pathology - January 25, 2024 Category: Pathology Source Type: research
Ultrastructure and laboratory effect of mesenchymal stem cells in streptozotocin-induced type I diabetic rats
AbstractDiabetes is a worldwide leading cause of mortality and morbidity, and type 1 diabetes (T1D) is the most common chronic autoimmune disorder in children and young adults. There is currently no definitive treatment for T1D. Stem cell administration appeared as an experimental treatment showing an improvement in the clinical outcome and restoration of tissue architecture in many disorders. Mesenchymal stem cells (MSCs) showed their capacity to generate insulin-producing cells as a promising potential treatment for diabetic patients. The present work aimed to study the effect of MSCs on the ultrastructure and laboratory...
Source: Comparative Clinical Pathology - January 17, 2024 Category: Pathology Source Type: research
Exploring Echinacea purpurea ’s effect on wound healing in rats: FOXO1/MIP2 pathway modulation
AbstractThe extracellular matrix (ECM) is a crucial component in all human tissues. During injury, the ECM is damaged; wound healing phases as hemostasis, inflammation, proliferation, and remodeling result in ECM synthesized and return to its native state.Echinacea purpurea is one of the most important medicinal herbs and is well-known for its antioxidant, anti-inflammatory, and immunomodulatory activities. The main aim of this research was to investigate the anti-inflammatory and wound-healing properties ofEchinacea treatment in rats due to the need for more scientific evidence regarding the efficacy of this medicinal pla...
Source: Comparative Clinical Pathology - January 6, 2024 Category: Pathology Source Type: research
Multiple cartilaginous exostoses of rib in dog: a case report
AbstractOsteochondroma is a proliferative condition that affects cartilage and bone in rare cases. Osteochondroma of the ribs is uncommon in dogs, and its manifestation has the potential to induce compression and harm to the vasculature and internal organs of the thoracic cavity. The discoverable evidence of this condition was encountered during the dissection of the chest of a 5-year-old male dog of mixed breed, who had no previous record of illness. The salient features noted during the dissection process were the identification of four dome-shaped masses which evinced a solid consistency and were found to be located in ...
Source: Comparative Clinical Pathology - December 30, 2023 Category: Pathology Source Type: research
Antioxidant activities of aqueous leaf extract of Parquetina nigrescens in high-fat-diet-streptozotocin-induced diabetic male albino rats
This study carried out the structural elucidation of aqueous leaf extract ofP. nigrescens using HPLC. The study also investigated the antioxidant potentials of this extract in serum, liver, and kidney of diabetic male albino rats. The HPLC analysis revealed the presence of beta sitosterol, veraquensin, kaempferol, among others. Furthermore, aqueous leaf extract ofP. nigrescens significantly (P < 0.05) improved the activities of antioxidant enzymes (catalase, glutathione peroxidase, superoxide dismutase, and reduced glutathione). Aqueous leaf extract ofP. nigrescens may serve as an antioxidant to ameliorate activiti...
Source: Comparative Clinical Pathology - December 29, 2023 Category: Pathology Source Type: research
Circulating biomarkers of upstream kynurenine pathway and brain-derived neurotropic factor in the diagnosis of type 2 and gestational diabetes mellitus with depression
AbstractThe kynurenine pathway (KP) is the main catabolic pathway for the essential amino acid tryptophan (TRP). The imbalance between beneficial and harmful kynurenines was concerned with the pathogenesis and biological mechanisms of various disorders such as diabetes mellitus (DM), obesity, dyslipidemia, hypertension, depression, and schizophrenia. The present study aimed to determine the levels of biomarkers —TRP, indoleamine-2,3-dioxygenase (IDO), kynurenine (KYN) and brain-derived neurotrophic factor (BDNF) in type 2 diabetes mellitus (T2DM) and gestational diabetes mellitus (GDM) to find their association with rele...
Source: Comparative Clinical Pathology - December 26, 2023 Category: Pathology Source Type: research
Altered body weight gain and selected blood constituents of Baluchi lambs fed dried pomegranate peels supplemented ration
This study aimed to investigate the effects of feeding dried PoP supplemented ration on body weight gain and selected blood parameters of male Baluchi lambs. Eighteen lambs were randomly assigned to control and two treatment groups, receiving rations with 5% and 10% PoP, respectively, for 60 days. Lambs ’ weight and blood samples were collected to assess blood parameters and liver enzymes. Results showed a significant reduction in weight gain in treatment groups (18% and 13%) compared to the control group (26%). Although there were no significant differences in blood biochemistry values except fo r an increase in GGT lev...
Source: Comparative Clinical Pathology - December 12, 2023 Category: Pathology Source Type: research
Pathogenic effects of Hepatozoon canis (Apicomplexa: Hepatozoidae) on pet dogs (Canis familiaris) with amplification of immunogenetic biomarkers
AbstractHepatozoon species (phylum Apicomplexa: Adeleorina, Hepatozooidae) are the cause of canine hepatozoonosis, a vector-borne disease (VBD) spread by ticks (Ixodidae). The aim of the present study was to determine the prevalence ofHepatozoon canis (H. canis) infections in different pet dogs admitted to different clinics in Egypt as well as determine the effect ofH. canis on dogs through analysis of the immunogenic genes and the oxidative stress markers. One hundred dogs with different clinical signs, fever, fatigue, tick infestation around the ears and neck region, were examined. Thin blood films were performed and sta...
Source: Comparative Clinical Pathology - December 10, 2023 Category: Pathology Source Type: research
Erythrocyte morphology variations in late pregnant ewes and their influence on erythrocyte indices
AbstractThe periparturient period in small ruminants is considered crucial due to major endocrine, metabolic, and behavioral changes. These physiological variations can affect the normal hematological values of the animal, as well as the number and morphology of erythrocytes. The aim of this study was to determine the different morphological changes of erythrocytes that can occur in ewes during late pregnancy and their influence on erythrocytic index values. Our experimental study included 100Rembi ewes during late pregnancy that were apparently healthy. Blood samples were collected in an EDTA tube for each studied subject...
Source: Comparative Clinical Pathology - December 1, 2023 Category: Pathology Source Type: research
Assessing evening primrose oil effects on the serum levels of omentin-1 and biochemical parameters in the diabetic rat model
In this study, the influence of evening primrose oil on the serum levels of omentin-1 and some of biochemical parameters in the diabetic rat model were investigated. In total, 24 healthy Wistar Albino male rats with a weight range of 200 –250 g were classified into 4 groups of 6: First group: control normal, second group: EPO (evening primrose oil), third group: diabetic, fourth group: diabetic+EPO. After 20 days, serum samples were taken, and the required parameters were measured and analyzed. ELISA was utilized to determining serum omentin-1 levels. Also, the levels of biochemical factors TG, CHOL, HDL, LDL, FBS, and...
Source: Comparative Clinical Pathology - November 29, 2023 Category: Pathology Source Type: research
